BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 867.07 bits (2239), Expect = 0.000e+0
Identity = 446/869 (51.32%), Postives = 598/869 (68.81%), Query Frame = 0

Query:  112 LPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRT-EDQP-EARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYV------RAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLEN--GSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRL 970
            LP+S++ILLE+ +R  +   V + D++ I+NW+     N EI F P RV++QDFTGVPAIVD AAMRDAV KLGGD  KINP  P +LVIDHS+ VD+    +AL KN +LEF+RN ER+ FL+WG S+F N  ++PP +GIVHQVN+EYLAR  FT  +      +PD++VGTDSHTTM+NGLGV+GWGVGGIEAEA MLGQ I+M++P+VVG+++ G       +TD+VLT+T+ LR +GVVG+FVEFYG G+ HL +ADRATI+NM PEYGAT G FP+D  ++ YLRLT RE  +++ ++EY     + R  ED+  +  ++  + LD+GTVV S +GPKRPQDR+A+TDM     + +      + +A   + K   V       I  D   + L  G+V IAAITSCTNTSNP V++ +GLLA+ AV KGLTV P++KTSL+PGS VVT YL+ + ++  LD LGF +VGYG  TCIGNSGPL + +      GDL  + VLSGNRNFEGRI+   + N+LASPPLV+AYA+AG + +D   + LG D     ++L+DIWP++ EIQ V    I  +MF+  YS V  G+ERW ++   +   Y W+ +STYI++PP+F  MS++  + K IKNA  L  LG S T DHISPAG    + PA +YL  + ++ ++FNSYGSRRGN  +M RGTFAN+RL N+   G  G  T +      M ++DA+M+Y++    LIV+AGK+YG GSSRDWAAKG  LLGV+AV+ ESYERIHRSNLVGMG++PLQ+ EGES  S G +GKE +S+E   N    + +D+ V    G  IK  +  VR DT  E  YY+HGGIL+Y++R++
Sbjct:   34 LPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRGKNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTD----LVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPI-----IAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDG-SIKSFKATVRFDTELELTYYRHGGILNYMIRKV 892          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 710.294 bits (1832), Expect = 0.000e+0
Identity = 392/898 (43.65%), Postives = 574/898 (63.92%), Query Frame = 0

Query:  112 LPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQ-------------------------------ALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPE-ARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSE--KTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGP-LPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLG-QDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITES-DRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSH-GLTGKEIYSIEGLENGSRH--VDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRR 969
            LP+SL++LLE  +R        +++ Q  + W       D       RV++QDFTGVPA++DLAA+R++V K GG    ++ L PA+LV+DH V VDY    Q                               AL  N   EFERN ER+ FL+W  SAF N  V+PP TG++HQVN+EYLAR V  +   G V  +PD++VGTD+HTTM+NGLGVLGWGVG ++A+A M G  +T+  P+VVG ++KG + + +T+TDLVL +T+ LR  GV G FVEF+G  L  L +ADRATI+NM PEYGA  G FPIDE T+ YL  +GR+   IE V++Y     MFR+ D+ E  R+ + +E+D+  ++P ++GPKR +D++++ +++   K+A +++   K      G      A  +  DG  FT+  G V +AAI+SCTNTSNP V++ +GLLA+ AV  GL+V   VKTSLAPGS VVT YL+++ +M  L  LGF++VGYGC+TC  NSGP LP++I  A++ G+L    +LSGNRNFEGR+  DI+ N+LASP +V+AYA+AG I +++ ++PLG     G  +FLK+IWPS++EIQE+    +   ++++ YS V  G++ W ++ +  S D++ W+SDSTYI+ P Y   +    S+ +  KN R L  LGD+IT+DHISPAGS    SPA +YL+  G+A +++NSYG+RRGN+ VM+RGTF+N+RL+N L     G +T + P +   +I++AS +Y+ +GV L  I GK +G G+SRDWA +G  LLGV+A++  S++  +RSNLV  G+LPLQ     + +S+  L G E  SI+  +N +    +D+VV    GK  +  + +VR+D   E D +  GG++  ++ +
Sbjct:   40 LPYSLRVLLECAVR-------KRSETQGQV-W------KDVYERILRRVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQ---GGVL-FPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQ--GHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYIKEIG---SNARNWKNMRCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLL-NKVGPYTLHLPSEVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQI----TSISYESLKGTECLSIDIRDNITPKCLIDVVV----GKAERSFKAVVRVDNDYETDVFHAGGVIAQIVSK 905          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 129.028 bits (323), Expect = 2.035e-30
Identity = 147/526 (27.95%), Postives = 225/526 (42.78%), Query Frame = 0

Query:  153 IAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTL-VGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMN-FLASPPLVVAYALAGNIQVDMHKD----PLGQDQQGNDVFLKDIWPSQ 672
            +   P RV MQD T   A+  L  +   + K+           P+ +  DH +     G     +K+     + NAE Y FL    + +       P +GI+HQ+ +E  A              +P  L VGTDSHT    GLG L  GVGG +A   M      +  P+V+G KL GK+    +  D++L V  +L   G  G  VE++GEG+  +      TI NM  E GAT  +FP +     YL  T R  D   L  +Y   + +  + D    +Y   +E+D+ T+ P + GP  P     + ++ EK                            ++G    ++DG      I SCTN+S   +   + ++ + A+AKG+  K      + PGS+ +   +E+  L   + + G  V+   C  CIG      + I    +N     + V S NRNF GR  A+   + F+ SP + VA +LAG++  D   D    P G + + N  F  D  PSQ
Sbjct:  672 LRLRPDRVAMQDATAQMAM--LQFISSGLPKVA---------VPSTIHCDHLIQAQIEG-----KKDLARAKDLNAEVYNFLSTSGAKY-GVGFWKPGSGIIHQIILENYA--------------FPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRS-DIANLADKY---REVLLSPDG-GCKYDQVVEIDLNTLEPHVNGPFTPDLATPIGELGEKAK--------------------------KNGWPLEVKDG-----LIGSCTNSSYEDMARXTAII-KQALAKGMKSKS--NFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWD--RQNITKGEKN-----TIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAIKAPDGSELKLNAPF-GDELPSQ 1119          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000009029 (supercontig:LSalAtl2s:LSalAtl2s566:257091:257615:-1 gene:EMLSAG00000009029 transcript:EMLSAT00000009029 description:"augustus_masked-LSalAtl2s566-processed-gene-2.7")

HSP 1 Score: 76.6406 bits (187), Expect = 1.083e-16
Identity = 35/76 (46.05%), Postives = 48/76 (63.16%), Query Frame = 0

Query:   26 LRFGVKASGCSGFSYFIELGKQVNEDDQVFDSHGVKVVIDKQSLELVDGTEIDYVQDDLSALFKFNNPKVTASCGC 101
            LR GVK  GC+G SY ++   Q  + D+     GVK+ IDK++   + GTE+DYV  +LSA F FNNP +  +CGC
Sbjct:   54 LRIGVKQRGCNGMSYVLDYAIQKEKHDEEVIQDGVKIFIDKKAQLSILGTEMDYVDSNLSAEFVFNNPNIKGTCGC 129          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|543763|sp|P37032.1|ACON_LEGPH (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=IP210; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Major iron-containing protein; Short=MICP; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1084.32 bits (2803), Expect = 0.000e+0
Identity = 544/867 (62.75%), Postives = 663/867 (76.47%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIE---GLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLAN 972
            I  LP+SLK+LLENLLR E+G  V+  DI+AI +W    T   EIAF P RV+MQDFTGVPA+VDLAAMR A+VK+GG+  KI+PL+P +LVIDHSVMVD F +  ALE N ++E ERN ERYEFLRWGQ AF NF+VVPP TGI HQVN+EYL +TV+  E +G++ AYPDTLVGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ++ML+P+V+GFKL GK+ EG TATDLVLTVTQMLR  GVVGKFVEFYG GL  LPLADRATI+NMAPEYGATCG FP+D+ET+ YL LTGR+   I LV+ YA AQGM+  +D  E  +T +L LD+G+V PSLAGPKRPQD++ L+ +  +   ++         + G    K      ++ + F ++ G V IAAITSCTNTSNP V++A+GL+A+ A+ KGL  KPWVK+SLAPGSKVVTDYL  A L   LD+LGF++VGYGCTTCIGNSGPLP+ I + V   DL VSSVLSGNRNFEGR+H  ++ N+LASPPLVVAYAL G    D+ ++P+GQD++GNDV+LKDIWPS +EI   V A ++  MF+K Y+ VFKGD  W AI  +    Y WN DSTYIQ+PP+F  +S  P  +KPIK A VLA  GDSITTDHISPAGS  A SPAG YL  +GV  KDFNSYGSRRGNH+VMMRGTFAN+R+RNE+ PG EGG TRY P    MSIYDA+M+YQEN   L++IAGKEYGTGSSRDWAAKGT LLGVKAV+TES+ERIHRSNL+GMG+LPLQFKEG ++ +  L G E  SIE    L  G+  V + +  + G +I++IE L RIDT  E +YY++GGIL YVLR++++
Sbjct:   37 INRLPYSLKVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDLAAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNKERYEFLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENDGQLYAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQMLRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGRDKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSLPVEFNNFL--------IEVGKEKEKEKTFAVKNKD-FQMKHGHVVIAAITSCTNTSNPSVLMAAGLVAKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNSGPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCSDLSREPIGQDKEGNDVYLKDIWPSNEEIAAEV-AKVSGTMFRKEYAEVFKGDAHWQAIQTSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGSIKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTRYVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESFERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAM-VPVTIERQDG-DIEKIETLCRIDTADELEYYKNGGILQYVLRKISS 891          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|88909584|sp|Q8FTA8.2|ACNA_COREF (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1072.38 bits (2772), Expect = 0.000e+0
Identity = 528/919 (57.45%), Postives = 669/919 (72.80%), Query Frame = 0

Query:  102 ALPDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKD-----------RDAKAGDVLTKSAIE-----------------------------ITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTR--YQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLE-----NGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            A+P  +K   LP+SLK+L ENLLRTE+G ++++  I+AI NWDA A P+ EI FTPARV+MQDFTGVP +VDLA MR+AV  LGGD  K+NPL PAE+VIDHSV+V+ FG P ALEKN E+E+ERN ERY+FLRWG  AF NF+VVPP TGIVHQVNIEYLAR VF    + +  AYPDT +GTDSHTTM NGLG+LGWGVGGIEAEAAMLGQ ++ML+P+VVGFKL G++P G TATD+VLT+T+MLR+ GVV KFVEFYG G+  +PLA+RATI NM+PE+G+TC +FPIDEET+ YLRLTGR ++QI LV+ YA AQGM+  +D PEA Y+  LELD+ TVVPS+AGPKRPQDRI L++  E+  E ++AY  D           R A  G     S  +                             ++  G  +T++ G V IA+ITSCTNTSNP V++ +GL+AR A  KGL  KPWVKT  APGS+VV  Y ++A+L  DL+ LGF + G+GCTTCIGNSGPLPE I  A+   DL  ++VLSGNRNFEGRI  D+KMN+LASP +V+AYA+AG +  D   + LGQDQ GNDVFLKDIWPS +EI+E + A I++ +++  Y++VFKGD++W  + I     + W+ +STYI+  PYF GM+ +P  +  I+NARVLA LGDS+TTDHISPA S    +PA +YL   GV R+D+NS GSRRGNH+VMMRGTFAN+RL+N+L     GG+TR   Q    +  IYDA + Y+E G+PL+V+AGKEYGTGSSRDWAAKGT LLGV+AV+TES+ERIHRSNL+GMGV+PLQF EGES  S GL G E + I GL         + V +    E+G++++  + +VRIDTP E DY++HGGIL YVLR++A S
Sbjct:   28 AVPGMEK---LPYSLKVLGENLLRTEDGANITEEHIEAIANWDASADPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVKTLGGDPDKVNPLNPAEMVIDHSVIVEAFGRPDALEKNVEIEYERNEERYQFLRWGSEAFSNFRVVPPGTGIVHQVNIEYLARVVF----DNDGLAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPVGVTATDVVLTITEMLRDHGVVQKFVEFYGNGVKSVPLANRATIGNMSPEFGSTCAMFPIDEETIKYLRLTGRPEEQIALVEAYAKAQGMWLEQDAPEAEYSEYLELDLSTVVPSIAGPKRPQDRILLSEAKEQFREDLKAYTNDPVQVDQSIPAKRMANEGGFQPGSTSDLDNYNASWPGEGESAAANAEGRPSNPVTVVSPQGGEYTIDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAEKGLKSKPWVKTICAPGSQVVDGYYQRADLWKDLEALGFYLSGFGCTTCIGNSGPLPEEISEAINEHDLAATAVLSGNRNFEGRISPDVKMNYLASPIMVIAYAIAGTMDFDFENEALGQDQDGNDVFLKDIWPSTEEIEETIQAAISRELYEADYADVFKGDKQWQELDIPSGKTFEWDENSTYIRKAPYFDGMTAEPQPVTDIENARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDAHGVERQDYNSLGSRRGNHEVMMRGTFANIRLQNQLV-DIAGGYTRDFTQEGGPQAFIYDACVNYKEAGIPLVVLAGKEYGTGSSRDWAAKGTNLLGVRAVITESFERIHRSNLIGMGVVPLQFPEGESHESLGLDGTETFDITGLTALNEGTTPKTVKVTATKENGEKVE-FDAVVRIDTPGEADYFRHGGILQYVLRQMAAS 937          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81622450|sp|Q9I3F5.1|ACNA_PSEAE (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1070.46 bits (2767), Expect = 0.000e+0
Identity = 546/878 (62.19%), Postives = 674/878 (76.77%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSA-------------IEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSR-HVDLV--VNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRL 970
            +  LP SLK+LLENLLR E+G  V+  D++A+  W  +   + EI + PARV+MQDFTGVPA+VDLAAMR A+ K GGD QKINPL+P +LVIDHSVMVD F +  A E+N E+E +RN ERY FLRWGQ+AFDNF VVPP TGI HQVN+EYL RTV+TK+E+G   A+PDTLVGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ++ML+P+V+GFKL GK+ EG TATDLVLTVTQMLR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCG FP+DE TL YLRL+GR +  ++LV+ Y+  QG++R E   E  +T TL LDMG V  SLAGPKRPQDR+AL +++    E++         + G +L++               I+   DG T  L++GAV IAAITSCTNTSNP V++A+GLLA+ AV KGL  KPWVK+SLAPGSKVVTDY + A L   LD+LGFD+VGYGCTTCIGNSGPL E I  A++  DLTV+SVLSGNRNFEGR+H  +K N+LASPPLVVAYALAG++++++ ++PLG  + G  V+LKDIWPSQKEI E +   ++  MF K Y+ VF GDE+W AI + +SD Y W +DSTYIQ+PP+F  +++ P  I  ++ ARVLA LGDS+TTDHISPAG+  ADSPAG+YL E GV  KDFNSYGSRRGNH+VMMRGTFAN+R++NE+  G EGG T Y P   +++IYDA+M+YQE+G PL+++AGKEYGTGSSRDWAAKGT LLGVKAV+ ES+ERIHRSNLVGMGVLPLQF+ G+ + S  LTGKE+ +I GL    + H+ L   V  E G +    ++L RIDT  E +Y++ GGILHYVLR +
Sbjct:   35 LGKLPMSLKVLLENLLRWEDGSTVTGDDLKALAGWLRERRSDREIQYRPARVLMQDFTGVPAVVDLAAMRAAMAKAGGDPQKINPLSPVDLVIDHSVMVDKFASESAFEQNVEIEMQRNGERYAFLRWGQNAFDNFSVVPPGTGICHQVNLEYLGRTVWTKDEDGRTYAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGKLREGITATDLVLTVTQMLRKKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGFFPVDEITLGYLRLSGRPESTVKLVEAYSKEQGLWR-EKGHEPVFTDTLHLDMGEVEASLAGPKRPQDRVALQNVASAFNEFLGLQLHPSSTEEGRLLSEGGGGTAVGANAAFGEIDYQHDGQTHRLKNGAVVIAAITSCTNTSNPSVMMAAGLLAKKAVEKGLQRKPWVKSSLAPGSKVVTDYFKAAGLTRYLDELGFDLVGYGCTTCIGNSGPLLEPIEKAIQQADLTVASVLSGNRNFEGRVHPLVKTNWLASPPLVVAYALAGSVRINLSEEPLGTGKDGQPVYLKDIWPSQKEIAEAIQ-KVDTEMFHKEYAEVFAGDEKWQAIQVPQSDTYEWQADSTYIQHPPFFEHIAEAPPAIADVEQARVLAVLGDSVTTDHISPAGNIKADSPAGRYLREHGVEPKDFNSYGSRRGNHEVMMRGTFANIRIKNEMLGGEEGGNTLYVPSGEKLAIYDAAMRYQEDGTPLVIVAGKEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFENGQDRKSLKLTGKEVLNIRGLGGELKPHMPLSVEVTREDGSQ-DSFKVLCRIDTLNEVEYFKAGGILHYVLRSM 909          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|88909586|sp|Q4JVM4.1|ACNA_CORJK (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1068.91 bits (2763), Expect = 0.000e+0
Identity = 533/914 (58.32%), Postives = 669/914 (73.19%), Query Frame = 0

Query:  102 ALPDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTD----MSEKTAEYVRAYAK----DRDAKA---------------------GDVLTKSA---------IEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTR--YQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLE---NGS--RHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRL 970
            A+P  +K   LP+SLK+L ENLLR E+G ++++  I+AI NWD  A PN EI FTPARV+MQDFTGV  IVDLA +RDAVV LGGD   +NPL PAE+VIDHSV+++ FG   ALEKN E+E++RN ERY+FLRWG  AF+NF+VVPP TGIVHQVNIEYLAR+VF  + NG   AYPDT VGTDSHTTM NGLG+LGWGVGGIEAEAAMLGQ I+ML+P+VVGFKL G++P G TATD+VLT+T MLR  GVVGKFVEFYG+G+  LPLA+RATI NM+PE+G+T  +FPIDEET+ YL LTGR+ + +E V+ YA AQGM+  + + E  Y+  LELD+ TVVPS+AGPKRPQDRI L D      +    YV A A     D DA+                      G++ + +A         + +  +G    L+ G V IA+ITSCTNTSNP V+V +GLLARNA AKGL   PWVKTS+APGS+VV  Y EKA L  DL+ +GF +VGYGCTTCIGNSGPLPE I   +  GDL  ++VLSGNRNFEGRI+ D+KMN+LASP LV+AYA+AG +  D    PLGQDQ GNDVFLKDIWPS ++I+EV+ ++I + ++ + Y+NVF+GDERW ++ +     ++W+  STYI+  PYF GMSK+P  +  +K ARVLA LGDS+TTDHISPA +    +PA +YL   GV RKD+NS G+RRGNH+VM+RGTFAN+RL+N+L  G  GG+TR   Q    +  IYDA+M YQ+   PL+V+ GKEYGTGSSRDWAAKGTLLLGVKAV+ ES+ERIHRSNL+GMGV+PLQF EGES  S G+ G E + IEG+E   NGS  + V +    E+G++I+  + + RIDTP E DYY++GGIL +VLR +
Sbjct:   28 AVPGMEK---LPYSLKVLGENLLRNEDGKNITREHIEAIANWDPSAEPNFEIQFTPARVIMQDFTGVACIVDLATIRDAVVALGGDADDVNPLNPAEMVIDHSVIIEAFGDSDALEKNVEIEYQRNDERYKFLRWGTGAFENFRVVPPGTGIVHQVNIEYLARSVF--DNNG--LAYPDTCVGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPISMLIPRVVGFKLTGEIPTGVTATDVVLTITDMLRQHGVVGKFVEFYGKGVGELPLANRATIGNMSPEFGSTAAMFPIDEETVKYLELTGRDQETLERVEAYAKAQGMW-LDPEKEVEYSEYLELDLSTVVPSIAGPKRPQDRIELNDSKAQFRKDLHNYVEADASAVTPDFDAEGPATENTSAQTAGTPASAADAKGNIPSAAAGAEGRPSNPVTVNYNGEDIELDHGMVAIASITSCTNTSNPSVMVGAGLLARNAAAKGLKSAPWVKTSMAPGSQVVNGYYEKAGLWKDLEAMGFYLVGYGCTTCIGNSGPLPEEISAGINEGDLAATAVLSGNRNFEGRINPDVKMNYLASPILVIAYAIAGTMDFDFETQPLGQDQDGNDVFLKDIWPSTEDIEEVIASSITKDLYAEDYANVFEGDERWRSLDVPSGKTFDWDPKSTYIRKAPYFDGMSKEPEAVNDVKGARVLALLGDSVTTDHISPASTIKPGTPAAQYLDANGVERKDYNSLGARRGNHEVMVRGTFANIRLQNQLLDGVSGGYTRDFTQEGGPQSFIYDAAMNYQKENTPLVVLGGKEYGTGSSRDWAAKGTLLLGVKAVIAESFERIHRSNLIGMGVVPLQFPEGESWKSLGIEGTETFDIEGIEELNNGSTPKTVKVTATKENGEKIE-FDAVTRIDTPGEADYYRNGGILQFVLRNM 932          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|302595583|sp|A0QX20.1|ACNA_MYCS2 (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1064.29 bits (2751), Expect = 0.000e+0
Identity = 539/917 (58.78%), Postives = 670/917 (73.06%), Query Frame = 0

Query:  102 ALPDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDR----DAKAGDVLTKS-------AIEITQDG---------------------------NTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTR--YQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLE-----NGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            A+P  +K   LP+SLK+L ENLLRTE+G +++K  I+AI NWD  A P+ EI FTPARV+MQDFTGVP IVDLA MR+AV  LGGD  K+NPLAPAELVIDHSV++D FG   A E+N ELE+ERNAERY+FLRWGQ AFD+FKVVPP TGIVHQVNIEYLARTV  ++      AYPDT VGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQ ++ML+P+VVGFKL G++  G TATD+VLTVT MLR  GVVGKFVEFYG+G+A +PLA+RAT+ NM+PE+G+T  IFPIDEET+ YLRLTGR D+Q+ LV+ YA AQGM+   ++ E  ++  LELD+ TVVPS++GPKRPQDRI LTD      + +  Y +      + K  + + +S       ++    DG                             F L+ GAV +A ITSCTNTSNP V++ + LLA+ AV KGLT KPWVKT++APGS+VVTDY  KA L   L+KLG+ + GYGCTTCIGN+GPLPE I  A+ + DL V++VLSGNRNFEGRI  D+KMN+LASPPLV+AY +AG +  D   DPLGQD +GNDVFL+DIWPS  EI+E + ++IN+ MF +SY++VFKGD+RW ++P  E D + W+  STY++  PYF GM  +P  +  IK ARVLA LGDS+TTDHISPAG+    +PA +YL   GV RKD+NS GSRRGNH+VM+RGTFAN+RLRN+L     GG+TR   QP   +  IYDAS  Y++ G+PL+V+ GKEYG+GSSRDWAAKGT+LLGVKAV+TES+ERIHRSNL+GMGV+PLQF  GES  S  L G E Y IEG+E        + V +    E G +++  + +VRIDTP E DYY++GGIL YVLR +  S
Sbjct:   34 AVPGTEK---LPYSLKVLAENLLRTEDGANITKDHIEAIANWDPNAEPSIEIQFTPARVIMQDFTGVPCIVDLATMREAVAALGGDPNKVNPLAPAELVIDHSVILDVFGNASAFERNVELEYERNAERYQFLRWGQGAFDDFKVVPPGTGIVHQVNIEYLARTVMVRDG----VAYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLSGEIKPGVTATDVVLTVTDMLRRHGVVGKFVEFYGKGVAEVPLANRATLGNMSPEFGSTAAIFPIDEETINYLRLTGRTDEQLALVEAYAKAQGMWHDPER-EPVFSEYLELDLSTVVPSISGPKRPQDRIELTDAKNAFRKDIHNYVEQNHPTPETKLDEAVEESFPASDPVSLSFADDGAPDMRPSAANGATGRPTNPVLVHSEERGDFVLDHGAVVVAGITSCTNTSNPSVMLGAALLAKKAVEKGLTTKPWVKTNMAPGSQVVTDYYNKAGLWPYLEKLGYYLGGYGCTTCIGNTGPLPEEISKAINDNDLAVTAVLSGNRNFEGRISPDVKMNYLASPPLVIAYGIAGTMDFDFESDPLGQDSEGNDVFLRDIWPSAAEIEETIASSINREMFTESYADVFKGDDRWRSLPTPEGDTFEWDPASTYVRKAPYFDGMPAEPEPVSDIKGARVLALLGDSVTTDHISPAGAIKPGTPAAQYLDANGVERKDYNSLGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTQPGGPQAFIYDASENYKKAGIPLVVLGGKEYGSGSSRDWAAKGTVLLGVKAVITESFERIHRSNLIGMGVIPLQFPAGESAASLKLDGTETYDIEGIEELNSGKTPKTVHVTATKEDGSKVE-FDAVVRIDTPGEADYYRNGGILQYVLRNMLKS 941          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|30173505|sp|P70920.2|ACNA_BRADU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1063.52 bits (2749), Expect = 0.000e+0
Identity = 523/894 (58.50%), Postives = 649/894 (72.60%), Query Frame = 0

Query:  102 ALPDQDK-----IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEV--------TAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEK-TAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGT-----EGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSR-----HVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            +LP  +K     I+ LP+S+K+LLENLLR E+G  V KADI A+  W  K +   EIAF PARV+MQDFTGVPA+VDLAAMR+A+ KLGGD +KINPL P +LVIDHSV+V++FG  +A  KN   E+++N ERYEFL+WGQ+AF NF VVPP TGI HQVN+EYL++TV+TK+E   V         AYPD+LVGTDSHTTMVNGL VLGWGVGGIEAEA MLGQ ++ML+P VVGFKLKG M EG TATDLVLTVTQMLR LGVVGKFVEF+G GL HL +AD+ATIANMAPEYGATCG FP+D   + YL+ +GR   ++ LV+ YA AQG+FRT    +  +T TL LD+  VVPS+AGPKRP+ RIAL  ++E  +      Y K  +          A     +G  + +  G V IAAITSCTNTSNP V++ +GLLARNA AKGL  KPWVKTSLAPGS+VV  YL  + L   LDK+GF++VG+GCTTCIGNSGPLPE I  ++ +  +  ++VLSGNRNFEGR+  D++ N+LASPPLVVA+ALAG++  ++  +PLG+ + G  V+LKDIWP+ KEI   +   +  ++FKK Y++VFKGD  W  I   ES+ Y WN  STY+QNPPYF GM K+P  +  I  AR+LA  GD ITTDHISPAGS    SPAGKYL+E  V   DFN YG+RRGNH+VMMRGTFAN+R++N +  G      EGG T++ P+  +MSIYDA+MKYQ+  VPL+V AG EYG GSSRDWAAKGT LLGV+AV+ +S+ERIHRSNLVGMGVLPL F+EG S  S GL G E  ++ GL    +       ++V  D S   ++R+ LL RIDT  E DYY++GGILHYVLR+LA
Sbjct:   24 SLPTAEKNGLKGISKLPYSMKVLLENLLRNEDGRSVKKADIVAVSKWLRKKSLEHEIAFRPARVLMQDFTGVPAVVDLAAMRNAMQKLGGDAEKINPLVPVDLVIDHSVIVNFFGDNKAFAKNVTEEYKQNQERYEFLKWGQAAFSNFSVVPPGTGICHQVNLEYLSQTVWTKKEKMTVGKKTGTFEVAYPDSLVGTDSHTTMVNGLAVLGWGVGGIEAEACMLGQPLSMLLPNVVGFKLKGAMKEGVTATDLVLTVTQMLRKLGVVGKFVEFFGPGLDHLSVADKATIANMAPEYGATCGFFPVDAAAIDYLKTSGRAAPRVALVQAYAKAQGLFRTAKSADPVFTETLTLDLADVVPSMAGPKRPEGRIALPSVAEGFSVALANEYKKTEE---------PAKRFAVEGKKYEIGHGDVVIAAITSCTNTSNPSVLIGAGLLARNAAAKGLKAKPWVKTSLAPGSQVVAAYLADSGLQAHLDKVGFNLVGFGCTTCIGNSGPLPEEISKSINDNGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAHALAGSVTKNLAVEPLGEGKDGKPVYLKDIWPTSKEINAFMKKFVTASIFKKKYADVFKGDTNWRKIKTVESETYRWNMSSTYVQNPPYFEGMKKEPEPVTDIVEARILAMFGDKITTDHISPAGSIKLTSPAGKYLSEHQVRPADFNQYGTRRGNHEVMMRGTFANIRIKNFMLKGADGNIPEGGLTKHWPDGEQMSIYDAAMKYQQEQVPLVVFAGAEYGNGSSRDWAAKGTRLLGVRAVICQSFERIHRSNLVGMGVLPLTFEEGTSWSSLGLKGDEKVTLRGLVGDLKPRQKLTAEIVSGDGS---LQRVSLLCRIDTLDELDYYRNGGILHYVLRKLA 905          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81522761|sp|Q8ZP52.1|ACNA_SALTY (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=2-methyl-cis-aconitate hydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=RNA-binding protein)

HSP 1 Score: 1063.14 bits (2748), Expect = 0.000e+0
Identity = 540/871 (62.00%), Postives = 657/871 (75.43%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTE-DQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEY--VRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLEN----GSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLAN 972
            IA LP SLK+LLENLLR ++G  V+  DIQA+  W   A  + EIA+ PARV+MQDFTGVPA+VDLAAMR+AV +LGGD  K+NPL+P +LVIDHSV VD+FG   A E+N  LE ERN ERY FL+WG+ AF  F VVPP TGI HQVN+EYL + V+++ ++GE  AYPD+LVGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ++ML+P VVGFKL GK+ EG TATDLVLTVTQMLR  GVVGKFVEFYG+GL  LPLADRATIANM+PEYGATCG FPID  TL Y+RL+GR DD +ELV+ YA AQGM+R   D+P   +T+TLELDMG V  SLAGPKRPQDR+AL D+ +  A    +      RD        +  ++ T +G  + L DGAV IAAITSCTNTSNP V++A+GLLA+ AV  GL  +PWVK SLAPGSKVV+DYL +A L   LD+LGF++VGYGCTTCIGNSGPLPE I  A++ GDLTV +VLSGNRNFEGRIH  +K N+LASPPLVVAYALAGN+ +++  DPLG D++G+ V+LKDIWPS +EI   V   ++  MF+K Y+ VF+G E W +I +  SD Y W SDSTYI+  P+F  M   P+ +K I  AR+LA LGDS+TTDHISPAGS   DSPAG+YL   GV RKDFNSYGSRRGNH+VMMRGTFAN+R+RNE+ PG EGG TR+ P    MSIYDA+M YQ+   PL VIAGKEYG+GSSRDWAAKG  LLG++ V+ ES+ERIHRSNL+GMG+LPL+F +G ++ + GLTG+E+  I  L+N     +  V L  +D S + +       RIDT  E  YYQ+ GILHYV+R + N
Sbjct:   35 IARLPKSLKVLLENLLRWQDGESVTDEDIQALAGWLKNAHADREIAWRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTSKVNPLSPVDLVIDHSVTVDHFGDDDAFEENVRLEMERNHERYMFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDSLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAITLEYMRLSGRSDDLVELVETYAKAQGMWRNPGDEPV--FTSTLELDMGDVEASLAGPKRPQDRVALGDVPKAFAASAELELNTAQRD--------RQPVDYTMNGQPYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAQAKLTPYLDELGFNLVGYGCTTCIGNSGPLPEPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLATDPLGYDRKGDPVYLKDIWPSAQEIARAVEL-VSSDMFRKEYAEVFEGTEEWKSIQVESSDTYGWQSDSTYIRLSPFFDEMQAQPAPVKDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQNHGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMLPGVEGGMTRHLPGTEAMSIYDAAMLYQQEKTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEVIDIADLQNLRPGATIPVTLTRSDGSKETVP---CRCRIDTATELTYYQNDGILHYVIRNMLN 891          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81624827|sp|Q9RTN7.1|ACNA_DEIRA (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein; Flags: Precursor)

HSP 1 Score: 1052.35 bits (2720), Expect = 0.000e+0
Identity = 535/863 (61.99%), Postives = 654/863 (75.78%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTK-EENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYS--IEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLR 968
            ++ LP S+K+LLE++LR  N  DV + D++ +  W A   P  EI F PARV++QDFTGVPA+VDLAAMR A+VKLGGD  KINPL P +LVIDHSV VD FGT  AL  N  LEFERN ERYEFLRWGQ AFDNF VVPP +GIVHQVN+EYLA+ V ++ E++GEV  YPD+LVGTDSHTTM+NGLG++GWGVGGIEAEA MLGQ I ML+P+V+GFK+ G MPEGATATDL L VTQMLR  GVVGKFVEFYG GL+++ L DRATIANMAPEYGAT G FP+D+E L YLR TGR +D+I LV+ Y  AQGMFRT++ P+  +T T+ELD+ T+VPSLAGPKRPQDR+ L+DM     E + A  K+R  + G    K   + T  G    +  GAVT+A+ITSCTNTSNP V++A+GL+A+ AV KGL  KPWVKTSLAPGS+VVT+YLE A L   LD++GF+ VGYGC TCIGNSGPLPE +  A++ GDL V+SVLSGNRNFEGR++  IK N+LASPPLVVAYALAG +  D+  D +GQD  G DVFLKDIWP+  EIQE +  +IN  MFKK Y  + K +  W AIP+ E   ++W  DSTYIQNPP+F  ++    +I+ IK AR L  +GDS+TTDHISPAGSF AD+PAG+YLTE+G+A KDFNSYGSRRGN ++M RGTFAN+RL+N+LAPGTEGG+T         SI+DAS  Y+E GVPL+V+AGK+YG GSSRDWAAKGT LLGVKAV+ ES+ERIHRSNLVGMGVLPLQ+K GE+  S G+ G E +   + G     + V + V  + G   + I ++ RIDTP E DYY++GGIL  VLR
Sbjct:   37 VSRLPVSIKVLLESVLREANDYDVRREDVETVAGWSA-TNPEVEIPFKPARVILQDFTGVPAVVDLAAMRSAMVKLGGDPSKINPLIPVDLVIDHSVQVDEFGTEFALANNMALEFERNRERYEFLRWGQQAFDNFGVVPPASGIVHQVNLEYLAKGVQSRAEDDGEVV-YPDSLVGTDSHTTMINGLGIVGWGVGGIEAEAVMLGQPIYMLMPEVIGFKITGAMPEGATATDLALRVTQMLREKGVVGKFVEFYGAGLSNMTLPDRATIANMAPEYGATMGFFPVDDEALRYLRRTGRLEDEIGLVEAYYKAQGMFRTDETPDPVFTDTIELDLATIVPSLAGPKRPQDRVNLSDMHSVFNEALTAPVKNRGFELGS--DKLDAQGTIGGTDIKIGHGAVTLASITSCTNTSNPSVLIAAGLVAKKAVEKGLKTKPWVKTSLAPGSRVVTEYLETAGLQQYLDQIGFNTVGYGCMTCIGNSGPLPEPVVEAIQEGDLVVASVLSGNRNFEGRVNPHIKANYLASPPLVVAYALAGTVVNDIVNDAIGQDSNGQDVFLKDIWPTNAEIQEAMDRSINAEMFKKVYDGIEKSNADWNAIPVAEGALFDWKEDSTYIQNPPFFDTLAGGAHEIESIKGARALVKVGDSVTTDHISPAGSFKADTPAGRYLTERGIAPKDFNSYGSRRGNDRIMTRGTFANIRLKNQLAPGTEGGFTTNFLNGEVTSIFDASTAYKEAGVPLVVLAGKDYGMGSSRDWAAKGTFLLGVKAVIAESFERIHRSNLVGMGVLPLQYKNGETADSLGINGDETFEFVLPGDLKPRQDVTVKVTGKDGN-TRDITVMCRIDTPVEIDYYKNGGILQTVLR 894          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|166215073|sp|P25516.3|ACNA_ECOLI (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=RNA-binding protein; AltName: Full=Stationary phase enzyme)

HSP 1 Score: 1049.27 bits (2712), Expect = 0.000e+0
Identity = 529/870 (60.80%), Postives = 657/870 (75.52%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTE-DQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEY----VRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKE---IYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRL 970
            I  LP SLK+LLENLLR ++G  V++ DI A+  W   A  + EIA+ PARV+MQDFTGVPA+VDLAAMR+AV +LGGD  K+NPL+P +LVIDHSV VD FG  +A E+N  LE ERN ERY FL+WG+ AF  F VVPP TGI HQVN+EYL + V+++ ++GE  AYPDTLVGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ++ML+P VVGFKL GK+ EG TATDLVLTVTQMLR  GVVGKFVEFYG+GL  LPLADRATIANM+PEYGATCG FPID  TL Y+RL+GR +DQ+ELV++YA AQGM+R   D+P   +T+TLELDM  V  SLAGPKRPQDR+AL D+ +  A      V A  KDR            ++   +G+ + L DGAV IAAITSCTNTSNP V++A+GLLA+ AV  GL  +PWVK SLAPGSKVV+DYL KA L   LD+LGF++VGYGCTTCIGNSGPLP+ I  A++  DLTV +VLSGNRNFEGRIH  +K N+LASPPLVVAYALAGN+ +++  +P+G D++G+ V+LKDIWPS +EI   V   ++  MF+K Y+ VF+G   W  I +T SD Y W  DSTYI+  P+F  M   P+ ++ I  AR+LA LGDS+TTDHISPAGS   DSPAG+YL  +GV RKDFNSYGSRRGNH+VMMRGTFAN+R+RNE+ PG EGG TR+ P+   +SIYDA+M+Y++   PL VIAGKEYG+GSSRDWAAKG  LLG++ V+ ES+ERIHRSNL+GMG+LPL+F +G ++ + GLTG+E   I  ++ L+ G+     +   +  +E+  +    RIDT  E  YYQ+ GILHYV+R +
Sbjct:   35 ITRLPKSLKVLLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEP--IFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDR----------QPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRKGDPVYLKDIWPSAQEIARAV-EQVSTEMFRKEYAEVFEGTAEWKGINVTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV--VPCRCRIDTATELTYYQNDGILHYVIRNM 889          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81564851|sp|Q6NH63.1|ACNA_CORDI (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1047.73 bits (2708), Expect = 0.000e+0
Identity = 525/906 (57.95%), Postives = 660/906 (72.85%), Query Frame = 0

Query:  112 LPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEK----TAEYVRAYAKDRDAKAGDVLTKSAIE----------------------------------ITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMS-IYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLE---NG--SRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            LP+SLK+L ENLLRTE+G +++   I AI NWD  A P+ EI FTPARV+MQDFTGVP +VDLA MR+AV  LGGD  K+NPL PAE+VIDHSV+++ FG+  AL KN E+E+ERN ERY+FLRWG  AF NF+VVPP TGIVHQVNIE LAR VF  + NG   AYPDT +GTDSHTTM NGLG+LGWGVGGIEAEAAMLGQ ++ML+P+VVGFKL G++P G TATD+VLT+T+MLR  GVV KFVEFYG G+  +PLA+RATI NM+PE+G+TC IFPIDEET+ Y+ LTGR ++Q+ LV+ YA AQGM+  +D PEA Y+  LELD+ TVVPS+AGPKRPQDRI LT+  E+      +Y  A   D    A  + ++ A++                                   + +G  +TL+ G V IA+ITSCTNTSNP V++ +GL+AR A AKGL  KPWVKT  APGS+VV  Y ++A+L  DL+ LGF + G+GCTTCIGNSGPLPE I  A+   DLT ++VLSGNRNFEGRI  D+KMN+L SP +V+AYA+AG +  D    PLGQD  GNDVFLKDIWPS +EI+E +   I++ +++  Y++VFKGDE+W  +P  E   ++W+  STYI+  PYF GM+ +P+ +  IK ARVLA LGDS+TTDHISPA S    +PA +YL E GVAR D+NS GSRRGNH+VMMRGTFAN+RL+N+L     GG+TR   ++ E + I+DA   Y+  G+PL+VIAGKEYGTGSSRDWAAKGT LLGVKAV+TES+ERIHRSNL+GMGV+PLQF  GES  S GL G E + IEG+E   NG   + V +    ESG +++  + +VRIDTP E DYY++GGIL YVLR + N+
Sbjct:   35 LPYSLKVLGENLLRTEDGANITADHINAIANWDPSAEPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVKTLGGDPDKVNPLNPAEMVIDHSVIIEAFGSTLALAKNVEIEYERNEERYQFLRWGSKAFSNFRVVPPGTGIVHQVNIENLARVVF--DNNG--LAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPAGVTATDVVLTITEMLREHGVVQKFVEFYGNGVKSIPLANRATIGNMSPEFGSTCAIFPIDEETVKYMHLTGRSEEQVALVEAYAKAQGMWLEQDAPEAEYSEYLELDLSTVVPSIAGPKRPQDRILLTEAKEQFRKDLPDYCSAEPVDESLPAKRMDSEGAVQKEGEDVAGYNSSRAGHGESAAEGAAGRQSNPVVVSSPNGGEYTLDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAAKGLKAKPWVKTICAPGSQVVDGYYKRADLWKDLEALGFYLSGFGCTTCIGNSGPLPEEISAAINENDLTATAVLSGNRNFEGRISPDVKMNYLVSPIMVIAYAIAGTMDFDFETQPLGQDIDGNDVFLKDIWPSTEEIEETIAGAISRELYEADYADVFKGDEQWQNLPTPEGKTFDWDEKSTYIRKAPYFDGMTMEPAPVSDIKGARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDENGVARNDYNSLGSRRGNHEVMMRGTFANIRLQNQLV-DIAGGYTRDFTKNGEQAFIFDACQNYKAAGIPLVVIAGKEYGTGSSRDWAAKGTNLLGVKAVITESFERIHRSNLIGMGVIPLQFPAGESHASLGLDGTETFDIEGIEELNNGVTPKTVHVTATKESGDQVE-FDAVVRIDTPGEADYYRNGGILQYVLRNMINA 934          

BLAST of aconitate hydratase vs. nr
Match: gi|1085192357|gb|OGT79362.1| (aconitate hydratase 1 [Gammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_61_13])

HSP 1 Score: 1231.47 bits (3185), Expect = 0.000e+0
Identity = 594/863 (68.83%), Postives = 709/863 (82.16%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            +ATLP+SLKILLENLLR E+G+++   DI+A+  WD KA P+ EIAFTPARV+MQDFTGVPA+VDLAAMRDA+ +LGGD  +INPL+PAELVIDHSV VD FG+  A E+NAE+EFERN ERYEFLRWGQ AF NFKVVPP TGIVHQVN+E+LART+F +E+NG + A+PDT+VGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ITML+PQV+GF+L G++PEGATATDLVLTVTQMLR  GVVGKFVEF+G GLA LPLADRATIANMAPEYGATCGIFPIDEETL YLRL+GRED QI LV+ YA  QGM+R    P ARYT T+ LDMG++ P LAGPKRPQDR+ L D+ + + + +      R   AGD   +SA  +   G +F ++DGAV IAAITSCTNTSNP V+V +GLLA+ A  +GLT KPWVKTSLAPGS VV DYL+KA L   LD++GF++VGYGCTTCIGNSGPLPEAI   +R  DL VS+VLSGNRNFEGRIHA++KMN+LASPPLVVAYA+AGN+ VD+  D LG D  G  V+L+DIWP+QKEI + + A IN  MF+KSY NV++GD RW+ + +  ++RY W  DSTY+QNPPYFVGMSK P  IK IK ARVLA LGDSITTDHISPAG+   DSPAG+YL E GVA  DFNSYGSRRGNH+VMMRGTFAN+RLRN L  G EGG T++ P   ++SIYDASM+YQ++ VPL+++AGKEYGTGSSRDWAAKGTLLLGV+AV+ ES+ERIHRSNLVGMGVLPL +++GE+  S GL G E+Y+IEGL+ G+  V +V  D+SGK  KR++  VR+DTP+EW+YY +GGILHYVLR+LA
Sbjct:   32 VATLPYSLKILLENLLRFEDGINIEAGDIEALAKWDPKAEPDKEIAFTPARVLMQDFTGVPAVVDLAAMRDAMARLGGDPARINPLSPAELVIDHSVQVDVFGSANAAERNAEIEFERNQERYEFLRWGQQAFSNFKVVPPDTGIVHQVNLEFLARTIFAQEKNGVLEAFPDTVVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPITMLIPQVIGFELTGRLPEGATATDLVLTVTQMLRKKGVVGKFVEFFGSGLATLPLADRATIANMAPEYGATCGIFPIDEETLRYLRLSGREDQQIALVEAYAKEQGMWRDPAAPVARYTDTMTLDMGSIEPCLAGPKRPQDRVTLRDVRKVSQDRMGEMQAARKGGAGD---RSA-SVNHGGKSFEVKDGAVVIAAITSCTNTSNPSVMVGAGLLAKKARERGLTAKPWVKTSLAPGSLVVRDYLDKAGLTPYLDQMGFNIVGYGCTTCIGNSGPLPEAISEGIRKEDLVVSAVLSGNRNFEGRIHAEVKMNYLASPPLVVAYAIAGNMNVDLQNDSLGNDAAGKPVYLRDIWPTQKEINDAMAAAINSGMFRKSYGNVYQGDARWSGLKVPSTNRYEWRGDSTYVQNPPYFVGMSKAPPGIKQIKGARVLAKLGDSITTDHISPAGNIKKDSPAGRYLVEHGVAPADFNSYGSRRGNHEVMMRGTFANIRLRNLLLTGVEGGMTKHMPSGEQLSIYDASMRYQKDNVPLVILAGKEYGTGSSRDWAAKGTLLLGVRAVLAESFERIHRSNLVGMGVLPLTYRDGENAESLGLAGTEVYTIEGLKEGATEVTVVATDDSGKS-KRLQARVRVDTPKEWEYYTNGGILHYVLRQLA 889          

BLAST of aconitate hydratase vs. nr
Match: gi|739473592|ref|WP_037333493.1| (aconitate hydratase AcnA [Salinisphaera hydrothermalis] >gi|666936519|gb|KEZ78951.1| aconitate hydratase [Salinisphaera hydrothermalis C41B8])

HSP 1 Score: 1230.7 bits (3183), Expect = 0.000e+0
Identity = 602/880 (68.41%), Postives = 708/880 (80.45%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIE---------------ITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            +A LP++ KILLENLLR E+G +V+  DI+A+ NWDAKA P+ EIAFTPARVV+QDFTGVPA+VDLAAMRDA+  LGG   KINP +PAELVIDHSVMVD +G+ +A + NA+LEF RN ERY FLRWGQ AFDNFKVVPP TGIVHQVN+EYLAR VF  E+ G   AYPDTLVGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQ ITML+PQV+GFKL GK+ EG TATDLVLTVT+MLR  GVVGKFVEFYG+GLA L LADRATIANMAPEYGATCGIFP+DEET+ Y+ LTGR+ +Q+EL +EYA AQG++R   QP+A YT +LELD+ TV PSLAGPKRPQDR+ L+DM       V+ + + R+ KAG  + ++  E               +      F L DG+V IAAITSCTNTSNP V++ +GLLARNA+ KG+TVKPWVKTSLAPGSKVVT+YLEKA L  DLD+LGF++VGYGCTTCIGNSGPLP+ IG AVRN +L V+SVLSGNRNFEGR+H D++MNFLASPPLVVAYALAG I  D+  DPLGQD  GNDVFL D+WPSQKEI + +  ++N  MFK+SY +VF GD RW  + + E + Y W+ DSTY+QNPPYF GM+ D  DI  IK AR LA LGDSITTDHISPAG+   DSPAG+YL EQGV   DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGGWTRYQPE  EM IYDA+MKY++ G PL+++AGKEYGTGSSRDWAAKGTLLLGVKAV+ ES+ERIHRSNLVGMGVLPLQF EG+S  S GL G E + ++GL  G++ V +    ESG+ ++  +  VRIDTP+EWDYYQ+GGILHYVLR+LA +
Sbjct:   33 VAKLPYAQKILLENLLRHEDGANVTADDIKALANWDAKAEPDTEIAFTPARVVLQDFTGVPAVVDLAAMRDAMKNLGGSPAKINPQSPAELVIDHSVMVDEYGSDKAFDLNAKLEFNRNKERYAFLRWGQGAFDNFKVVPPDTGIVHQVNLEYLARVVFGNEDTG--LAYPDTLVGTDSHTTMINGVGVLGWGVGGIEAEAAMLGQPITMLIPQVIGFKLTGKLAEGTTATDLVLTVTEMLRAKGVVGKFVEFYGDGLADLALADRATIANMAPEYGATCGIFPVDEETIRYMELTGRDAEQLELTREYAKAQGLWREAGQPDANYTDSLELDLSTVQPSLAGPKRPQDRVLLSDMQATYKREVKPFIEAREEKAGKSMAEAKEESEGGIPSKSVGGTARVNYKDAEFDLHDGSVVIAAITSCTNTSNPAVMIGAGLLARNAINKGMTVKPWVKTSLAPGSKVVTEYLEKAGLDDDLDQLGFNLVGYGCTTCIGNSGPLPDPIGEAVRNHNLNVASVLSGNRNFEGRVHGDVRMNFLASPPLVVAYALAGTIDTDLTNDPLGQDADGNDVFLADLWPSQKEIYDTIGTSLNSEMFKQSYGDVFAGDSRWQNLDVPEGEIYAWDEDSTYVQNPPYFEGMTVDVDDIPTIKGARCLAKLGDSITTDHISPAGAITQDSPAGQYLQEQGVKPADFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGWTRYQPEGREMFIYDAAMKYKDEGTPLVILAGKEYGTGSSRDWAAKGTLLLGVKAVIAESFERIHRSNLVGMGVLPLQFNEGDSHESLGLDGSETFDLDGLAAGAKSVHITATKESGETVE-FDARVRIDTPKEWDYYQNGGILHYVLRQLAKA 909          

BLAST of aconitate hydratase vs. nr
Match: gi|1180041561|ref|WP_083562433.1| (aconitate hydratase AcnA [Oceanococcus atlanticus] >gi|1178157080|gb|ORE86237.1| aconitate hydratase 1 [Oceanococcus atlanticus])

HSP 1 Score: 1229.54 bits (3180), Expect = 0.000e+0
Identity = 583/880 (66.25%), Postives = 698/880 (79.32%), Query Frame = 0

Query:  102 ALPDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKA----------GDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            A+  Q ++  LP+SLK+LLENL+R E+GV+++K DI A+INWD KA P+ EIAFTPARV++QDFTGVP +VDLAAMRDAV KLGGD  KINPL P ELVIDHSVMVD  G+  AL+ NA++EF+RN ERY FLRWGQ+AFDNFKVVPP TGIVHQVNIEYLAR VF KE      AYPDT+VGTDSHTTMVNG+GVLGWGVGGIEAEAAMLGQ +TML+PQVVG++L GK+ EG TATDLVLT+ + LR LGVVGKFVEF+GEGLA LPLADRATIANMAPEYGATCGIFP+DE T+ YL+LTGR++D++ LV+ YA A GM+R +D P+A Y+  LELD+ TV PSLAGPKRPQDRI L+DM      +++    +R  K+          G V+     ++  DG +F L DGAV IAAITSCTNTSNP V++ +GL+A+ A A+GL VKPWVKTSLAPGS+VVTDYL KA LM DL+ LGF VVGYGCTTCIGNSGPL  AI  A+++ DL  +SVLSGNRNFEGR+H+D++MNFLASPPLVVAYALAG + +D+  +PLGQDQ GNDV+LKDIWPS  EIQE + A++N  MFK  Y++V+ GD RW  + I     Y W++ STY+QNPPYF GMS D  DI  IK AR LA LGDSITTDHISPAG    D  AG+YL  +GV + DFNS GSRRGNH+VMMRGTFANVRLRN++APGTEGGWTR+ P+  +MSIY+A+MKY+    PL+V+ GKEYGTGSSRDWAAKGT+LLGVKAV+ ES+ERIHRSNLVGMGVLPLQF +G+S  S GLTG E Y IEGL+ G++ V +    + GK +   +  VRIDTP+EWDYY+HGGIL YV+R+LA
Sbjct:   25 AVASQAQLDRLPYSLKVLLENLVRHEDGVNITKDDINALINWDPKAEPSTEIAFTPARVILQDFTGVPCVVDLAAMRDAVAKLGGDATKINPLVPVELVIDHSVMVDKSGSNDALDLNAKIEFDRNKERYAFLRWGQTAFDNFKVVPPDTGIVHQVNIEYLARVVFGKETG---VAYPDTVVGTDSHTTMVNGIGVLGWGVGGIEAEAAMLGQPVTMLIPQVVGYRLTGKLSEGVTATDLVLTIVERLRELGVVGKFVEFFGEGLADLPLADRATIANMAPEYGATCGIFPVDEATINYLKLTGRDEDELALVETYAKANGMWRHDDDPDAEYSHVLELDLSTVQPSLAGPKRPQDRILLSDMKSSYQSHLKPMVAERSEKSQAEARLESEGGPVVKSGVAQLAIDGESFELSDGAVVIAAITSCTNTSNPVVLLGAGLVAQKAAARGLKVKPWVKTSLAPGSQVVTDYLNKAGLMDDLEALGFHVVGYGCTTCIGNSGPLKAAISQAIQDNDLVATSVLSGNRNFEGRVHSDVRMNFLASPPLVVAYALAGTVDIDLVNEPLGQDQDGNDVYLKDIWPSNAEIQETLAASLNSDMFKTRYADVYAGDARWQGLEIPTGKIYEWDNTSTYVQNPPYFEGMSLDTDDIGEIKGARCLAKLGDSITTDHISPAGVIKPDQDAGEYLMSKGVQQADFNSLGSRRGNHEVMMRGTFANVRLRNQIAPGTEGGWTRHLPDGEQMSIYEAAMKYKAENTPLVVLGGKEYGTGSSRDWAAKGTILLGVKAVICESFERIHRSNLVGMGVLPLQFADGDSHESLGLTGLETYDIEGLQRGAKSVSVTATTDDGK-VTTFQAKVRIDTPKEWDYYEHGGILQYVIRQLA 900          

BLAST of aconitate hydratase vs. nr
Match: gi|1085188777|gb|OGT76241.1| (aconitate hydratase 1 [Gammaproteobacteria bacterium RIFCSPLOWO2_12_47_11])

HSP 1 Score: 1220.3 bits (3156), Expect = 0.000e+0
Identity = 603/867 (69.55%), Postives = 699/867 (80.62%), Query Frame = 0

Query:  106 QDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNT-FTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            Q  I+ LP+SLKILLENLLR E+G +V KADI A+  WD KA P+ EIAFTPARV+MQDFTGVPA+VDLAAMRDA+ +LGGD +KINPL+PAELVIDHSVMVDYF  P A EKNAE+EFERN ERY FLRWGQ AF NFKVVPP TGI HQVN+EYLARTVFT+  NG   AYPDT+VGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ITML+PQVVGFKL GK+PEGATATDLVLTVT MLR  GVVGKFVE++G GL +LPLADRAT+ANMAPEYGATCGIFPID ETL YLRL+GR ++QI LV+ YA AQGM+R + + EA Y+  LELDMG VVPSLAGPKRPQDR+ALTDM +     +    + R        TK       DG T + L DGAV IAAITSCTNTSNP V++ +GLLA+ A   GLT KPWVKTSLAPGS VV+DYL+KA L+  L  LGFD VGYGCTTCIGNSGPLPEA+   +  GDL V+SVLSGNRNFEGR+HA+IKMNFLASPPLVVAY++AG +  D++KDPLG  + G  V+LKDIWPS +EI + + + I+  MF+ SY++VFKGD RW +I +  SDRY+W   STYIQNPPYFVGMSK       I+ ARVLA LGDSITTDHISPAG+   D PAGKYL E GV  KDFNSYG+RRGNH+VMMRGTFAN+RLRN+LAPGTEGGWT +QP   +M IYDASMKYQ  G PLI++AGKEYGTGSSRDWAAKGT+LLG +AV+ ES+ERIHRSNLVGMGVLPL FK+GE+  S GLTGKE+Y I+GL+NG++ V ++   +  KEI R +  VR+DTP+EW+YY +GGILHYVLR+LA
Sbjct:   29 QHDISRLPYSLKILLENLLRHEDGENVRKADIAALAEWDPKAEPDQEIAFTPARVLMQDFTGVPAVVDLAAMRDAMSQLGGDPKKINPLSPAELVIDHSVMVDYFAQPDAAEKNAEIEFERNHERYVFLRWGQEAFSNFKVVPPDTGICHQVNLEYLARTVFTRTVNGVEQAYPDTVVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPITMLIPQVVGFKLIGKLPEGATATDLVLTVTNMLRKKGVVGKFVEYFGPGLDNLPLADRATLANMAPEYGATCGIFPIDVETLNYLRLSGRSEEQITLVEAYAKAQGMWR-DSKLEANYSDVLELDMGRVVPSLAGPKRPQDRVALTDMQQTFKTSLPPMVEKR--------TKPGKATVTDGKTSYELTDGAVVIAAITSCTNTSNPSVMIGAGLLAKKAHEMGLTRKPWVKTSLAPGSLVVSDYLKKAGLVPHLAALGFDTVGYGCTTCIGNSGPLPEAVSKGIAEGDLVVASVLSGNRNFEGRVHAEIKMNFLASPPLVVAYSIAGTVNTDLNKDPLGTGKDGKPVYLKDIWPSSQEISKAIASTIDSKMFQGSYAHVFKGDTRWGSIKVPASDRYHWEDSSTYIQNPPYFVGMSKQAQGFDQIQGARVLALLGDSITTDHISPAGAIKKDGPAGKYLIEHGVLHKDFNSYGARRGNHEVMMRGTFANIRLRNQLAPGTEGGWTIHQPSGEQMFIYDASMKYQAEGTPLIILAGKEYGTGSSRDWAAKGTMLLGCRAVIAESFERIHRSNLVGMGVLPLTFKQGENAESLGLTGKEVYDIDGLKNGAKEVTVIAKGD--KEI-RFKATVRVDTPKEWEYYNNGGILHYVLRQLA 883          

BLAST of aconitate hydratase vs. nr
Match: gi|1268755610|ref|WP_099019639.1| (aconitate hydratase AcnA [Marinicella litoralis])

HSP 1 Score: 1218.76 bits (3152), Expect = 0.000e+0
Identity = 576/865 (66.59%), Postives = 689/865 (79.65%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENG-SRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLAN 972
            I+ LPFSLKILLENLLR ENG+DV+ +DI+A+ NWD  A P  EIAFTP+RVV+QDFTGVPA+VDLAAMRDA+  LGGDVQKINPL+PAELVIDHS+ VD +GT  + E N ++EF+RN ERY FL+WGQ+AFD FKVVPP  GIVHQVN+EYLAR +F  E+NGE+ AYPDT+VGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQAI+ML+PQVVGFKL GKM EG TATDLVLTV  MLR  GVVGKFVEF+G GL HLPLADRATIANMAPEYGATCGIFPID E+L YL L+GR ++Q+ LV+ YA AQGMF  E+  +A YTA LELDM TVVPS++GPKRPQDRI LT   E    + + +   R  K+        + ++ D   +   DG + +AAITSCTNTSNP V++ +GLLARNA AKGL VKPWVKTSLAPGSKVV+ YL  A +M DL+ LGF+VVG+GCTTCIGNSGPL  A+  A+ + DL  +SVLSGNRNFEGRIHADI+MNFLASPPLVVAYA+AG +  D+  D LG+DQ+GN V+L+DIWPS  EI + +  N+++  F + Y  +F+GD  W A+  TES+ ++W  DSTYI+NPPYF GM+ +   I  I  ARVLA LGDS+TTDHISPAG+   DSPAG YL E+GV + +FNSYGSRRGNH+VMMRGTFAN+RLRN+LAPGTEGGWTR+QP + EMSI+DASMKYQ  G PL+VI G EYGTGSSRDWAAKGT LLGVKAV+ +SYERIHRSNLVGMGV+PLQFK GES  ++ LTGKE+Y + GL+NG S+   + V+ E G  +   E+ VRIDTP+E +Y +HGGILHYVLR LA+
Sbjct:   32 ISRLPFSLKILLENLLRNENGIDVTVSDIEALCNWDPNAAPATEIAFTPSRVVLQDFTGVPAVVDLAAMRDAMKALGGDVQKINPLSPAELVIDHSIQVDKYGTMDSAEINTQIEFQRNMERYGFLKWGQTAFDTFKVVPPGNGIVHQVNLEYLARVIFGAEKNGELMAYPDTVVGTDSHTTMINGIGVLGWGVGGIEAEAAMLGQAISMLIPQVVGFKLTGKMQEGVTATDLVLTVVDMLREHGVVGKFVEFFGSGLQHLPLADRATIANMAPEYGATCGIFPIDNESLRYLTLSGRSEEQVNLVEAYAKAQGMFHDENSIDAEYTAVLELDMSTVVPSISGPKRPQDRIELTVAKETYNRHFKEFENGRAVKS--------VAVSSDRGNYDFTDGNIAVAAITSCTNTSNPAVMLGAGLLARNAAAKGLKVKPWVKTSLAPGSKVVSHYLNAAGVMDDLETLGFNVVGFGCTTCIGNSGPLDPALSKAINDNDLIATSVLSGNRNFEGRIHADIQMNFLASPPLVVAYAIAGTMDFDLQNDSLGEDQEGNPVYLRDIWPSNHEIADTIQNNVSKTSFSEGYEGIFEGDANWQAVKTTESELFDWQGDSTYIKNPPYFDGMTLEVGTIDNISGARVLAKLGDSVTTDHISPAGAIAEDSPAGSYLKEKGVPKVNFNSYGSRRGNHEVMMRGTFANIRLRNQLAPGTEGGWTRHQPSNEEMSIFDASMKYQAEGTPLLVIGGTEYGTGSSRDWAAKGTNLLGVKAVLVQSYERIHRSNLVGMGVIPLQFKAGESADTYDLTGKEVYDVSGLKNGESKTAQITVHREDGSTVS-FEVDVRIDTPKEVEYCRHGGILHYVLRNLAS 887          

BLAST of aconitate hydratase vs. nr
Match: gi|1148670134|ref|WP_077410279.1| (aconitate hydratase AcnA [Marinicella sp. F2])

HSP 1 Score: 1215.68 bits (3144), Expect = 0.000e+0
Identity = 581/865 (67.17%), Postives = 689/865 (79.65%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENG-SRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLAN 972
            I+ LPFSLKILLENLLR E+G+DV+  DI+A+ NWD KA P+ EIAFTP+RVV+QDFTGVPA+VDLAAMRDA+  LGGDVQKINPL+PAELVIDHS+ VD +GT  A + N ++EF+RN ERY FL+WGQ+AFD+FKVVPP  GIVHQVN+EYLAR +F  E+NGE+ AYPDT+VGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQAI+ML+PQVVGFKL GK+ EG TATDLVLTV  MLR  GVVGKFVEF+G GL HLPLADRATIANMAPEYGATCGIFP+D E+L YLRLTGR ++Q+ LV+ YA AQGMF  E   +A YTA LELDM TVVPS++GPKRPQDRI LT       ++   +   R  K+        I +      +   DG + +AAITSCTNTSNP V++ +GLLARNA AKGL VKPWVKTSLAPGSKVV+ YL  A LM DL+ LGF+VVG+GCTTCIGNSGPL  A+  A+ + DL  +SVLSGNRNFEGRIHADI+MNFLASPPLVVAYA+AG +  D++ D LG+DQ G+ V+LKDIWPS  EI + +  N+NQ  F + Y  +F+GD  W A+  TES+ ++W  DSTYI+NPPYF GM+ D   I  IK ARVLA LGDS+TTDHISPAG+  ADSPAG YL E+GV + +FNSYGSRRGNH+VMMRGTFAN+RLRN+LAPGTEGGWTR+QP D EMSI+DASMKYQ    PL+VI G EYGTGSSRDWAAKGT LLGVKAV+ +SYERIHRSNLVGMGV+PLQFK GES  ++ LTGKE Y I+GL+NG S+   + V+ + G  +   E+ VRIDTP+E +Y +HGGILHYVLR LA+
Sbjct:   32 ISRLPFSLKILLENLLRHEDGIDVTVDDIEALCNWDPKAEPSTEIAFTPSRVVLQDFTGVPAVVDLAAMRDAMKALGGDVQKINPLSPAELVIDHSIQVDKYGTNDAADLNTQIEFQRNMERYGFLKWGQTAFDSFKVVPPGNGIVHQVNLEYLARVIFGAEQNGELMAYPDTVVGTDSHTTMINGIGVLGWGVGGIEAEAAMLGQAISMLIPQVVGFKLTGKLQEGVTATDLVLTVVDMLREHGVVGKFVEFFGAGLQHLPLADRATIANMAPEYGATCGIFPVDNESLRYLRLTGRSEEQVNLVEAYAKAQGMFHDEHSLDAEYTAVLELDMSTVVPSISGPKRPQDRIELTVAKATYNQHFTEFENGRPVKS--------IPVKSAKGDYEFTDGNIAVAAITSCTNTSNPAVMLGAGLLARNAAAKGLKVKPWVKTSLAPGSKVVSHYLNSAGLMDDLESLGFNVVGFGCTTCIGNSGPLDPALSKAINDNDLIATSVLSGNRNFEGRIHADIQMNFLASPPLVVAYAIAGTMDFDLYNDALGKDQDGHPVYLKDIWPSDHEIADTIGNNVNQNSFAQGYEGIFEGDANWEAVKTTESELFDWQDDSTYIKNPPYFAGMTLDVGTIDNIKGARVLAKLGDSVTTDHISPAGAIAADSPAGLYLQEKGVEKANFNSYGSRRGNHEVMMRGTFANIRLRNQLAPGTEGGWTRHQPSDEEMSIFDASMKYQAEDTPLLVIGGTEYGTGSSRDWAAKGTNLLGVKAVLVQSYERIHRSNLVGMGVIPLQFKAGESADTYDLTGKETYDIDGLKNGASKTAQVTVHRDDGSTVT-FEVDVRIDTPKEVEYCRHGGILHYVLRDLAS 887          

BLAST of aconitate hydratase vs. nr
Match: gi|493969552|ref|WP_006912778.1| (aconitate hydratase AcnA [Salinisphaera shabanensis] >gi|543109851|gb|ERJ18122.1| Aconitase protein [Salinisphaera shabanensis E1L3A])

HSP 1 Score: 1214.13 bits (3140), Expect = 0.000e+0
Identity = 606/886 (68.40%), Postives = 700/886 (79.01%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNT---------------------FTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            IA LP++ KILLENLLR E+G +V   DI+A+ NWDAKA P+ EIAFTPARVV+QDFTGVPA+VDLAAMRDA+  LGG   KINPL+PAELVIDHSVMVD +GT +A + NA+LEF RN ERY FLRWGQ AFDNFKVVPP TGIVHQVN+EYLAR VF  E+     AYPDTLVGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQ ITML+PQVVGFKL GK+ EG TATDLVLTVT+MLR  GVVGKFVEF+G+GLA LPLADRATIANMAPEYGATCGIFP+D ET+ Y+ LTGR  +Q+ELV+ YA AQG++R E +P+A YT  LELDM TV PSLAGPKRPQDR+ L DM +     V  + K R  KA D   KS  E  Q                         F L DG+V IAAITSCTNTSNP V++ +GLLARNA+ +GL VKPWVKTSLAPGSKVVT+YLEKA L  DLDKLGF +VGYGCTTCIGNSGPLPE IG AVR  +L V+SVLSGNRNFEGR+H D++MNFLASPPLVVAYAL+G+I +DM+ DPLGQD  GNDV+L+DIWPSQKEI + +  ++N  MFK SY +VF GD RW  + + E + ++W+  STY+QNPPYF GMS D +DI  I+ AR LA LGDSITTDHISPAG+   DSPAG+YL E+GV+  DFNSYGSRRGNH+VMMRGTFANVRLRN LAPGTEGGWTR+QP   EM IYDA+MKY ++  PL+V+AGKEYGTGSSRDWAAKGTLLLGVK V+ +S+ERIHRSNLVGMGVLPLQFKEGE+  S GL G E + IEGLE+G+  V +    ESG +       VRIDTP+EWDYYQ+GGILHYVLR+LA +
Sbjct:   33 IAKLPYAQKILLENLLRHEDGSNVDADDIKALANWDAKAEPDTEIAFTPARVVLQDFTGVPAVVDLAAMRDAMANLGGSPDKINPLSPAELVIDHSVMVDEYGTDKAFDLNAKLEFNRNKERYAFLRWGQGAFDNFKVVPPDTGIVHQVNLEYLARVVFGNEDTN--LAYPDTLVGTDSHTTMINGVGVLGWGVGGIEAEAAMLGQPITMLIPQVVGFKLTGKLAEGCTATDLVLTVTEMLRAKGVVGKFVEFFGDGLADLPLADRATIANMAPEYGATCGIFPVDGETIRYMELTGRPAEQLELVEAYAKAQGLWREEGEPDADYTDVLELDMSTVQPSLAGPKRPQDRVLLADMQKTYRREVEPFIKARAEKA-DPEDKSMAEAKQQSEAGLTSDDIGGPVHAPVSYKETEFDLHDGSVVIAAITSCTNTSNPAVMIGAGLLARNAIQRGLQVKPWVKTSLAPGSKVVTEYLEKAGLNVDLDKLGFQLVGYGCTTCIGNSGPLPEPIGEAVREHNLNVASVLSGNRNFEGRVHGDVRMNFLASPPLVVAYALSGSIDIDMNNDPLGQDADGNDVYLRDIWPSQKEIYDTIGTSLNSEMFKDSYGDVFAGDSRWKGLDVPEGEIFDWDETSTYVQNPPYFEGMSVDVADIPTIQGARCLALLGDSITTDHISPAGAITKDSPAGQYLQEKGVSPSDFNSYGSRRGNHEVMMRGTFANVRLRNLLAPGTEGGWTRHQPSGDEMFIYDAAMKYADDKTPLVVLAGKEYGTGSSRDWAAKGTLLLGVKTVIAQSFERIHRSNLVGMGVLPLQFKEGENAESLGLDGTETFDIEGLESGATEVTVKATKESG-DTSEFTAKVRIDTPKEWDYYQNGGILHYVLRQLAKA 914          

BLAST of aconitate hydratase vs. nr
Match: gi|1356006784|ref|WP_105189370.1| (aconitate hydratase AcnA [Pseudoalteromonas sp. T1lg48])

HSP 1 Score: 1209.51 bits (3128), Expect = 0.000e+0
Identity = 579/892 (64.91%), Postives = 703/892 (78.81%), Query Frame = 0

Query:  104 PDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSA---------------------IEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIK-RIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            P  DK   LPFSLKILLENLLR E+G  ++K DIQA+++W  +A P+ E+AFTPARVVMQDFTGVPAIVDLAAMRDA+ +LGGD  KINPL+PAELVIDHSV VD +G   A + NA+LE++RN ERYEFLRWGQ+AFDN  VVPP TGIVHQVN+EYLAR VF K+E G   AYPD+LVGTDSHTTM+NGLG+LGWGVGGIEAEAAMLGQ I++L+PQVVGFKLKG +PEG TATDLVLTVTQ+LR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCGIFPID+ET+ YL+LT R D+Q+ L++ YA  QGM+R E   E  Y+  +ELD+GTVVPSLAGPKRPQDRI L    +   E++RA+  +R  K    + + A                      ++  +G  F L DGA  IAAITSCTNTSNP V++A+GL+A+ A   GL VKPWVKTSLAPGS+VVTDYL+KA LM DL+ LGF++VGYGCTTCIGNSGPLP+ I  A+    L VSS+LSGNRNFEGRIH D+KMNFLASPPLVVAYALAG   +D++ +PL QD QG DV+LKD+WPS  E+ E+V + + + MF KSY++V+KGDERW  I + ++ RY W+  STYI+  P+F GMSK+P  +  I  AR LA LGDS+TTDHISPAG+  AD+PAG+YL + GV++ DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGG TR QPED   SIYDA+M+YQ+N  PL+V+AG EYGTGSSRDWAAKG+LLLG+KAV+ +SYERIHRSNL+GMGVLPLQFK+G+     GLTGKE + I GL + ++ +++V  +E G++ K R++  VRIDTP+EW+YY+HGGIL YVLR++  S
Sbjct:   25 PLGDKAKRLPFSLKILLENLLRHEDGESINKDDIQALLDWQPQAKPSKEVAFTPARVVMQDFTGVPAIVDLAAMRDAMEQLGGDPSKINPLSPAELVIDHSVQVDSYGNNGAFDLNAKLEYQRNKERYEFLRWGQTAFDNLTVVPPATGIVHQVNLEYLARVVFEKDEQGHTFAYPDSLVGTDSHTTMINGLGILGWGVGGIEAEAAMLGQPISLLIPQVVGFKLKGALPEGTTATDLVLTVTQILRQKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGIFPIDQETIEYLKLTNRSDEQVALIEAYAKHQGMWR-EAGDEPLYSDIVELDLGTVVPSLAGPKRPQDRIPLDTAGDTITEHLRAFQDERAEKKALEMEEEARIVSEGGQPKQLQTEPEVTGIAKVKYNGEEFELSDGACVIAAITSCTNTSNPSVILAAGLVAKKAKELGLNVKPWVKTSLAPGSQVVTDYLKKAELMDDLEALGFNLVGYGCTTCIGNSGPLPDEIQQAITEHKLVVSSILSGNRNFEGRIHQDVKMNFLASPPLVVAYALAGRTDIDVYNEPLTQDVQGRDVYLKDLWPSVSEVAELVKSTVTKEMFAKSYADVYKGDERWRNIAVPQAKRYEWDEASTYIRKAPFFDGMSKEPPGLPSITGARCLAKLGDSVTTDHISPAGAIKADAPAGQYLIKHGVSQGDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGITRTQPEDDLASIYDAAMQYQKNNTPLVVLAGTEYGTGSSRDWAAKGSLLLGIKAVIAKSYERIHRSNLIGMGVLPLQFKDGQGHEELGLTGKETFDINGLCDKAKELEIVATNEDGQQTKFRVD--VRIDTPKEWEYYKHGGILQYVLRQMLRS 913          

BLAST of aconitate hydratase vs. nr
Match: gi|1356563504|ref|WP_105265156.1| (aconitate hydratase AcnA [Pseudoalteromonas sp. T1lg76])

HSP 1 Score: 1205.28 bits (3117), Expect = 0.000e+0
Identity = 579/889 (65.13%), Postives = 701/889 (78.85%), Query Frame = 0

Query:  107 DKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQ---------------------DGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIK-RIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            DK   LPFSLKILLENLLR E+G ++ K DIQA+++W  +A P+ E+AFTPARVVMQDFTGVPAIVDLAAMRDA+ +LGGD  KINPL+PAELVIDHSV VD +G   A + NA+LE++RN ERYEFLRWGQ+AFDN  VVPP TGIVHQVN+EYLAR VF  EE G   AYPD+LVGTDSHTTM+NGLG+LGWGVGGIEAEAAMLGQ I++L+PQVVGFKLKG +PEG TATDLVLTVTQMLR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCGIFPID+ET+ YL+LT R D+QI L++ YA  QGM+R E   E  Y+ T+ELD+GTVVPSLAGPKRPQDRI L +  +  + ++R +  +R  K    +   A  +++                     +G  F L DGA  IAAITSCTNTSNP V++A+GL+A+ A   GL VKPWVKTSLAPGS+VVTDYL+KA LM DL+ LGF++VGYGCTTCIGNSGPL + I  A+ +  L VSS+LSGNRNFEGRIH D+KMNFLASPPLVVAYA+AG   +D++ +PL QD+QG DV+LKD+WPS  E+ E+V + + + MF KSY++V+KGDERW  I + E+ RY W+  STYI+  P+F GMSK+P  +  I  AR LA LGDS+TTDHISPAG+  AD+PAG+YL E GV++ DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGG TR QPED   SIYDA++ YQ+N  PL+V+AG EYGTGSSRDWAAKG+LLLG+KAV+ +SYERIHRSNL+GMGVLPLQFK GE     GLTGKE + I GL + ++ +++V  +E G++ K R++  VRIDTP+EW+YY+HGGIL YVLR++  S
Sbjct:   28 DKAKRLPFSLKILLENLLRHEDGENIHKDDIQALLDWQPQAEPSKEVAFTPARVVMQDFTGVPAIVDLAAMRDAMEQLGGDPSKINPLSPAELVIDHSVQVDSYGNNGAFDINAKLEYQRNKERYEFLRWGQTAFDNLTVVPPATGIVHQVNLEYLARVVFENEEQGHTFAYPDSLVGTDSHTTMINGLGILGWGVGGIEAEAAMLGQPISLLIPQVVGFKLKGALPEGTTATDLVLTVTQMLREKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGIFPIDQETIEYLKLTNRSDEQIALIEAYAKHQGMWR-EAGDEFLYSDTVELDLGTVVPSLAGPKRPQDRIPLDEAGKIISAHLRHFEDERAEKTALGMEDEARIVSEGGQPRQSKTAANTCGIAKVKFNGEEFELSDGACVIAAITSCTNTSNPSVILAAGLVAKKAKELGLNVKPWVKTSLAPGSQVVTDYLKKAGLMDDLEALGFNLVGYGCTTCIGNSGPLADEIHQAIIDHKLIVSSILSGNRNFEGRIHQDVKMNFLASPPLVVAYAIAGRTDIDVYNEPLTQDEQGRDVYLKDLWPSVSEVAELVKSTVTKEMFAKSYADVYKGDERWRTIAVPEAKRYQWDEASTYIRKAPFFDGMSKEPPGLPSIAGARCLAKLGDSVTTDHISPAGAIKADAPAGEYLLEHGVSQGDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGITRTQPEDKLTSIYDAAIHYQKNDTPLVVLAGAEYGTGSSRDWAAKGSLLLGIKAVIAKSYERIHRSNLIGMGVLPLQFKAGEGHEELGLTGKETFDINGLCDKAKELEIVATNEDGQQTKFRVD--VRIDTPKEWEYYKHGGILQYVLRQMLRS 913          

BLAST of aconitate hydratase vs. nr
Match: gi|1356021809|ref|WP_105199946.1| (aconitate hydratase AcnA [Pseudoalteromonas sp. T1lg10])

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 580/891 (65.10%), Postives = 702/891 (78.79%), Query Frame = 0

Query:  107 DKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVL-----------------------TKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIK-RIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            DK   LPFSLKILLENLLR E+G ++ K DIQA+++W  +A P+ E+AFTPARVVMQDFTGVPAIVDLAAMRDA+ +LGGD  KINPL+PAELVIDHSV VD +G   A + NA+LE++RN ERYEFLRWGQ+AFDN  VVPP TGIVHQVN+EYLAR VF  EE G   AYPD+LVGTDSHTTM+NGLG+LGWGVGGIEAEAAMLGQ I++L+PQVVGFKLKG +PEG TATDLVLTVTQMLR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCGIFPID+ET+ YL+LT R D+QI L++ YA  QGM+R E   E  Y+ T+ELD+GTVVPSLAGPKRPQDRI L +  +  + ++R +  +R  +  +VL                       T    ++  +G  F L DGA  IAAITSCTNTSNP V++A+GL+A+ A   GL VKPWVKTSLAPGS+VVTDYL+KA LM DL+ LGF++VGYGCTTCIGNSGPL + I  A+ +  L VSS+LSGNRNFEGRIH D+KMNFLASPPLVVAYA+AG   +D++ +PL QD+QG DV+LKD+WPS  E+ E+V + + + MF KSY++V+KGDERW  I + E+ RY W+  STYI+  P+F GMSK+P  +  I  AR LA LGDS+TTDHISPAG+  AD+PAG+YL E GV++ DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGG TR QPED   SIYDA+M+YQ+   PL+V+AG EYGTGSSRDWAAKG+LLLG+KAV+ +SYERIHRSNL+GMGVLPLQFK GE     GLTGKE + I GL + ++ +++V  +E G++ K R++  VRIDTP+EW+YY+HGGIL YVLR++  S
Sbjct:   28 DKAKRLPFSLKILLENLLRHEDGENIHKDDIQALLDWQPQAEPSKEVAFTPARVVMQDFTGVPAIVDLAAMRDAMEQLGGDPSKINPLSPAELVIDHSVQVDSYGNNGAFDINAKLEYQRNKERYEFLRWGQTAFDNLTVVPPATGIVHQVNLEYLARVVFENEEQGHTFAYPDSLVGTDSHTTMINGLGILGWGVGGIEAEAAMLGQPISLLIPQVVGFKLKGALPEGTTATDLVLTVTQMLREKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGIFPIDQETIEYLKLTNRSDEQIALIEAYAKHQGMWR-EAGDEFLYSDTVELDLGTVVPSLAGPKRPQDRIPLDEAGKIISAHLRHFEDERAER--NVLGMEDEARIVSEGGQPRQSKTAANTCGIAKVKFNGEEFELSDGACVIAAITSCTNTSNPSVILAAGLVAKKAKELGLNVKPWVKTSLAPGSQVVTDYLKKAGLMDDLEALGFNLVGYGCTTCIGNSGPLADEIHQAIIDHKLIVSSILSGNRNFEGRIHQDVKMNFLASPPLVVAYAIAGRTDIDVYNEPLTQDEQGRDVYLKDLWPSVSEVAELVKSTVTKEMFAKSYADVYKGDERWRTIAVPEAKRYQWDEASTYIRKAPFFDGMSKEPPGLPSIVGARCLAKLGDSVTTDHISPAGAIKADAPAGEYLLEHGVSQGDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGITRTQPEDKLASIYDAAMQYQQTDTPLVVLAGAEYGTGSSRDWAAKGSLLLGIKAVIAKSYERIHRSNLIGMGVLPLQFKAGEGHEELGLTGKETFDINGLCDKAKELEIVATNEDGQQTKFRVD--VRIDTPKEWEYYKHGGILQYVLRQMLRS 913