BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 305/552 (55.25%), Postives = 404/552 (73.19%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPEN--YFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKA 550
            +  +++  ++ + +PLTLSEK+LY+HL D   ++  +RG+ Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLIQA+     DL  A   +AEV++FL +   KYG+GFWKPG+GIIHQ++LENYAFPG +M+GTDSHT N GGLG + IGVGGADAVDVMA + WELK PK+IGV+LTG +SGWT+PKDVILKVA ILTVKGGTGAIVEYFG G  ++SCTG GTICNMGAEIGATTS F Y+  M+ YL +T+R+D+A+ A++ +E       V  +P+    +DQV++IDL+ L P +NGPFTPDL+T +G ++  KA +N WP++V+ GLIGSCTNSSYED++R ++I +QAL KG+K K+   I PGSEQ+R T ERDG+     + G  +  NACGPCIGQW R +  K   KN+IV S+NRNF  R D NP TH FV SPE+T A+++AG LDF+P +D++   +G E++ + P G ELP +GF+  +  +  P ADGS V V V  DS RLQLL PF      + K +++LIKA
Sbjct:  631 LVQKLEIVKDRLNRPLTLSEKVLYSHL-DDPKNQDIVRGESYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIQAQIEGKKDLARAKDLNAEVYNFLSTSGAKYGVGFWKPGSGIIHQIILENYAFPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRSDIANLADKYRE-------VLLSPDGGCKYDQVVEIDLNTLEPHVNGPFTPDLATPIG-ELGEKAKKNGWPLEVKDGLIGSCTNSSYEDMARXTAIIKQALAKGMKSKSNFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWDRQNITK-GEKNTIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAIKAPDGSELKLNAPFGDELPSQGFDAGEDTYQHPPADGSSVSVTVDSDSNRLQLLTPFDKWDGKDHKEMRVLIKA 1172          

HSP 2 Score: 73.1738 bits (178), Expect = 2.706e-13
Identity = 38/89 (42.70%), Postives = 54/89 (60.67%), Query Frame = 0

Query:  641 SFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKA 729
            S A  LDF+P +D++   +G E++ + P G ELP +GF+  +  +  P ADGS V V V  DS RLQLL PF     +  K +++LIKA
Sbjct: 1084 SLAGSLDFDPRSDAIKAPDGSELKLNAPFGDELPSQGFDAGEDTYQHPPADGSSVSVTVDSDSNRLQLLTPFDKWDGKDHKEMRVLIKA 1172          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 146.747 bits (369), Expect = 3.003e-36
Identity = 160/517 (30.95%), Postives = 228/517 (44.10%), Query Frame = 0

Query:   28 WDGTPSKAFLRGKDY-VDFAPDRIACQDATAQMALLQF--MQAGKKKV---------AVPTTVHCDHLIQ---AKNGAA----ADLKFANSTSAEVFDFLE-SVSNKYGIGFWKPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPEN--YFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMN----------------------------VKATENEWPI----QVEWGLIGSCTNSSYEDLSR-ASSIAQQALDKGLKMKAEL--GINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKN------SIVHSFNRNFAKRADGNPNTHA-FVASPEITAAIAIAGRLDFN 470
            W+G       RGK+  + F P R+  QD T   A++ F  M+   KK+           PT +  DH IQ   A+   A     DL+F  +   E F FL+   S+   +    PG+GI+HQV LE  A          FP  + +GTDSHT    GLG+V  GVGG +A  VM G    +  P+++G R++G+        D++L + + L   G  G  VE++GPG   LS   + TI NM  E GAT   F  D     YLR T+R        +   HLT     Y +      F Q++ +DL  +    +GP  P    AV TDM                             + A +N+  I     V    I SCTN+S   +   A  +A++A+DKGL +   +   ++PGS  V +  +  G++   + LG  I       CIG      D      N      + V S NRNF  R   NP+T A ++ASP +  A AIAGR+D +
Sbjct:   65 WEGN------RGKNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRN--KERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSV-VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAV-TDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRI--NPHTRANYLASPPLVIAYAIAGRVDID 569          

HSP 2 Score: 56.6102 bits (135), Expect = 2.749e-8
Identity = 38/120 (31.67%), Postives = 61/120 (50.83%), Query Frame = 0

Query:  766 LTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF-DNESDYDL-IQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            ++G+   V D    YK      IV+   +YG GSSR+ AA  P  LGV AV+ +S+ RIH +NL   G++ L + + ES   L     + F+     +  P + L +++   DG+  + K
Sbjct:  746 ISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFK 865          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 131.339 bits (329), Expect = 1.841e-31
Identity = 138/446 (30.94%), Postives = 199/446 (44.62%), Query Frame = 0

Query:   92 ADLKFANSTS-----AEVFDFLESVSNKY-GIGFWKPGAGIIHQVVLENYA----FPGGMM-----IGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDR-----ADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGP-FTPDLSTA--VGTDMNVKATENEWP-----------------IQVEWGLIG----------SCTNSSYEDLSR-ASSIAQQALDKGLKMK--AELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSF-------------NRNFAKRADGNPNTHA-FVASPEITAAIAIAGRLDFN 470
            AD   +N TS      E F FL+ V++ +  I    PG G++HQV LE  A      GG++     +GTD+HT    GLG++  GVG  DA  VM G    L FP+++G+R+ G IS ++   D++L + + L   G  GA VE+FGP    LS   + TI NM  E GA    F  D    +YL  + R       V      VK   ++D     N    F  +I+IDLS + P ++GP  T D  +   +  D        E P                 I  E+  +G          SCTN+S   +   A  +A++A++ GL +    +  + PGS  V    +  G++     LG +I    C  C      Y+   N P +SIV +              NRNF  R D  P+  A ++ASP +  A AIAGR+D N
Sbjct:  158 ADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDE----NENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTC-----SYNSGPNLP-DSIVQAIKQGNLVCCGLLSGNRNFEGRLD--PDIRANYLASPLMVIAYAIAGRIDIN 591          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74856524|sp|Q54XS2.1|ACON_DICDI (RecName: Full=Probable aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 772.311 bits (1993), Expect = 0.000e+0
Identity = 377/647 (58.27%), Postives = 468/647 (72.33%), Query Frame = 0

Query:   14 KPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            KPLTL+EKI+Y HL D  PS    RG  Y+   PDR+A QDATAQMA+LQFM AG  + AVPTT+HCDHLI+A  G   DL+ A   + EV+DFL + + K+G+GFWKPG+GIIHQ+VLENYAFPGG+MIGTDSHT NAGGLGMVA+GVGGADAVDVMAG+ WELK PK+IGV+LTG++ GW++PKDVIL+VA+ILTVKGGTGAIVEYFG G  +LSCTG  TICNMGAEIGATTS F ++  M  YL +T R+++A+AAN  K +L AD      P  ++DQ+I+++L  L P +NGPFTPDL   + +        N WP +++ GLIGSCTNSSYED+SR++S+AQQALDKG+  KA+  I PGSEQ+R T ERDG + V EK+G  +  NACGPCIGQW R   PK   KNSI+ S+NRNF  R D N NTHAFVASPEI  A+ IAG + FNP+TD L +++G + +   PTG ELP +GF+  +  +  P  +G  + V V  +S RLQLL+PF P    +L  +++LIK             GPWL++RGHLDNI+NN LIGA+N+ N K N V NQ TGE   VP   R YK  G+  IVVGD NYGEGSSREHAA++PRHLG  A+LVKSFAR  + N
Sbjct:   49 KPLTLAEKIIYGHLED--PSTKVERGITYLKLHPDRVAMQDATAQMAVLQFMSAGLPETAVPTTIHCDHLIEAYKGGEKDLEVAKDINKEVYDFLSTSAKKFGMGFWKPGSGIIHQIVLENYAFPGGLMIGTDSHTPNAGGLGMVAVGVGGADAVDVMAGIPWELKAPKIIGVKLTGSLKGWSSPKDVILRVADILTVKGGTGAIVEYFGSGVESLSCTGMATICNMGAEIGATTSLFPFNKRMVDYLNSTGRSNIANAANSFKHNLVAD------PNAHYDQLIELNLDTLEPYINGPFTPDLGHPL-SKFAESVKTNNWPAELKVGLIGSCTNSSYEDMSRSASVAQQALDKGITAKAKFTITPGSEQIRATIERDGQMKVLEKVGGVVLANACGPCIGQWKREDVPK-GEKNSIITSYNRNFTGRNDSNVNTHAFVASPEIVTALTIAGDITFNPMTDFLTDKDGNKFKLTPPTGDELPSRGFDAGENTYQPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAGPWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPTVARDYKKRGVNWIVVGDENYGEGSSREHAALEPRHLGGKAILVKSFARIHETN 685          

HSP 2 Score: 246.128 bits (627), Expect = 1.451e-68
Identity = 126/250 (50.40%), Postives = 159/250 (63.60%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHA 875
            V   + A  + FNP+TD L +++G + +   PTG ELP +GF+  +  +  P  +G  + V V  +S RLQLL+PF P   + L  +++LIK  G           PWL++RGHLDNI+NN LIGA+N+ N K N V NQ TGE   VP   R YK  G+  IVVGD NYGEGSSREHAA++PRHLG  A+LVKSFARIHETNLKKQG+L LTF N SDYD I  DD  +   +   AP K LTL +  A
Sbjct:  483 VTALTIAGDITFNPMTDFLTDKDGNKFKLTPPTGDELPSRGFDAGENTYQPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAGPWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPTVARDYKKRGVNWIVVGDENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGILPLTFANPSDYDKISGDDRISIIGLKDLAPGKQLTLIVKSA 732          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74670431|sp|Q4WLN1.1|ACON_ASPFU (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; AltName: Full=Homocitrate dehydratase; Flags: Precursor)

HSP 1 Score: 766.918 bits (1979), Expect = 0.000e+0
Identity = 383/655 (58.47%), Postives = 464/655 (70.84%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M+  +D  R  + +PLT +EK+LY+HL D    +   RGK Y+   PDR+ACQDATAQMA+LQFM AG   VA PTTVHCDHLI+A+ G   DL  AN  + EV+DFL S + KY IGFWKPG+GIIHQ+VLENYAFPGG+MIGTDSHT NAGGL M AIGVGGADAVDVMAG+ WELK PK+IGV+LTG +SGWT PKDVILKVA +LTVKGGTGAI+EY GPG  +LSCTG GTICNMGAEIGATTS F ++  M  YL+AT R  + D A E  + L  D          +DQ+I+I+LS+L P +NGPFTPDL+T + +        N+WP +++ GLIGSCTNSSYED+SRA+SIA+ AL+ GLK K+   + PGSEQ+R T ERDG L   E+ G  I  NACGPCIGQW R  D K    NSI+ S+NRNF  R D NP THAFVASP++  A+ IAG L FNPLTD L +++G E     PTG  LP KG++     + AP AD S V VAV+  S+RLQLL  F P    +  G+ +LIK             GPWL++RGHLDNI+NN LIGAVNA N K N VKN+ TGE+  VP T R YKA G+K +V+GD NYGEGSSREHAA++PRHLG  A++ +SFAR
Sbjct:   53 MSENLDIVRRRLQRPLTYAEKVLYSHL-DDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPTTVHCDHLIEAQVGGDKDLARANEINKEVYDFLASATAKYNIGFWKPGSGIIHQIVLENYAFPGGLMIGTDSHTPNAGGLAMAAIGVGGADAVDVMAGLPWELKAPKVIGVKLTGEMSGWTTPKDVILKVAGLLTVKGGTGAIIEYHGPGVTSLSCTGMGTICNMGAEIGATTSMFPFNDRMYDYLKATKRQHIGDFAREYAKELREDEGA------EYDQLIEINLSELEPHINGPFTPDLATPI-SKFKEAVETNKWPEELKVGLIGSCTNSSYEDMSRAASIARDALNHGLKAKSLFTVTPGSEQIRATIERDGQLQTLEEFGGVILANACGPCIGQWDR-RDVKKGEPNSIISSYNRNFTGRNDANPATHAFVASPDLVVAMTIAGTLKFNPLTDKLKDKDGNEFLLQPPTGEGLPAKGYDPGRDTYQAPPADRSSVNVAVSPTSDRLQLLAGFEPWDGKDANGIPILIKCQGKTTTDHISMAGPWLKYRGHLDNISNNMLIGAVNAENGKANSVKNKFTGEYDAVPATARDYKARGVKWVVIGDWNYGEGSSREHAALEPRHLGGLAIITRSFAR 698          

HSP 2 Score: 248.44 bits (633), Expect = 3.337e-69
Identity = 130/253 (51.38%), Postives = 163/253 (64.43%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGT 878
            V+  + A  L FNPLTD L +++G E     PTG  LP KG++     + AP AD S V VAV+  S+RLQLL  F P   +   G+ +LIK  G           PWL++RGHLDNI+NN LIGAVNA N K N VKN+ TGE+  VP T R YKA G+K +V+GD NYGEGSSREHAA++PRHLG  A++ +SFARIHETNLKKQGML LTF + +DYD I  +DT +          KP+TL +   DG+
Sbjct:  501 VVAMTIAGTLKFNPLTDKLKDKDGNEFLLQPPTGEGLPAKGYDPGRDTYQAPPADRSSVNVAVSPTSDRLQLLAGFEPWDGKDANGIPILIKCQGKTTTDHISMAGPWLKYRGHLDNISNNMLIGAVNAENGKANSVKNKFTGEYDAVPATARDYKARGVKWVVIGDWNYGEGSSREHAALEPRHLGGLAIITRSFARIHETNLKKQGMLPLTFADPADYDKINPEDTVDLL-CTQLEVGKPMTLRVHPKDGS 752          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|586946283|sp|C8VG90.1|ACON_EMENI (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; AltName: Full=Homocitrate dehydratase; Flags: Precursor)

HSP 1 Score: 757.288 bits (1954), Expect = 0.000e+0
Identity = 384/687 (55.90%), Postives = 477/687 (69.43%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWE-LPP 675
            M+  +D  R  + +PLT +EKILY+HL D   ++   RGK Y+   PDR+ACQDATAQMA+LQFM AG   VA PTTVHCDHLI+A+ G   DL  AN  + EV+DFL S + KY IGFWKPG+GIIHQ++LENYAFPGG+MIGTDSHT NAGGL + AIGVGGADAVDVMAG+ WELK PK+IGVRLTG +SGWTAPKD+ILKVA +LTVKGGTGAI+EY GPG  +LS TG  TICNMGAEIGATTS F ++  M  YL+AT R  + D A    + L  D          +DQ+I+I+LS+L P +NGPFTPDL+T + +        N WP +++ GLIGSCTNSSYED+SRA+SIAQ ALD GLK K+   + PGSEQ+R T ERDG L   E+ G  I  NACGPCIGQW R  D K    NSIV S+NRNF  R D NP THAFV SP++  A++IAG L+FNPLTD+L +++G+E +   PTG  LP +G++     + AP  D S V VAV+  S+RLQLL  F P    +  G+ +LIK             GPWL++RGHLDNI+NN LIGAVNA N + N +KN  TGE+  VP T R YKA G+K +V+GD NYGEGSSREHAA++PRHLG  A++ +SFAR  + N     L  +    + F +P  ++ +PP
Sbjct:   51 MSENLDVVRRRLTRPLTYAEKILYSHL-DDPQNQDIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPTTVHCDHLIEAQLGGEKDLARANEINKEVYDFLASSTAKYNIGFWKPGSGIIHQIILENYAFPGGLMIGTDSHTPNAGGLAIAAIGVGGADAVDVMAGLPWELKAPKVIGVRLTGEMSGWTAPKDIILKVAGLLTVKGGTGAIIEYHGPGVNSLSATGMATICNMGAEIGATTSLFPFNDRMYDYLKATKRQQIGDFARSYAKDLREDEGA------EYDQLIEINLSELEPHINGPFTPDLATPI-SQFKEAVKANGWPEELKVGLIGSCTNSSYEDMSRAASIAQDALDHGLKAKSIFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDR-KDVKKGTPNSIVSSYNRNFTGRNDANPATHAFVTSPDLVVALSIAGTLNFNPLTDTLKDKDGKEFKLKAPTGDGLPSRGYDPGRDTYQAPPTDRSSVDVAVSPSSDRLQLLAGFQPWDGKDATGIPILIKCQGKTTTDHISMAGPWLKYRGHLDNISNNMLIGAVNAENGEANKIKNVFTGEYGAVPATARDYKARGVKWVVIGDWNYGEGSSREHAALEPRHLGGLAIITRSFARIHETN-----LKKQGMLPLTFSDPADYDRIPP 723          

HSP 2 Score: 249.595 bits (636), Expect = 1.275e-69
Identity = 130/258 (50.39%), Postives = 167/258 (64.73%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            V+  S A  L+FNPLTD+L +++G+E +   PTG  LP +G++     + AP  D S V VAV+  S+RLQLL  F P   +   G+ +LIK  G           PWL++RGHLDNI+NN LIGAVNA N + N +KN  TGE+  VP T R YKA G+K +V+GD NYGEGSSREHAA++PRHLG  A++ +SFARIHETNLKKQGML LTF + +DYD I  D T +       A DKP+TL +   DG    +K
Sbjct:  499 VVALSIAGTLNFNPLTDTLKDKDGKEFKLKAPTGDGLPSRGYDPGRDTYQAPPTDRSSVDVAVSPSSDRLQLLAGFQPWDGKDATGIPILIKCQGKTTTDHISMAGPWLKYRGHLDNISNNMLIGAVNAENGEANKIKNVFTGEYGAVPATARDYKARGVKWVVIGDWNYGEGSSREHAALEPRHLGGLAIITRSFARIHETNLKKQGMLPLTFSDPADYDRIPPDATVDLL-CTELAVDKPMTLRVHPKDGASFDVK 755          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|384872308|sp|O13966.2|ACON_SCHPO (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 756.518 bits (1952), Expect = 0.000e+0
Identity = 378/656 (57.62%), Postives = 474/656 (72.26%), Query Frame = 0

Query:    4 RVDKAREIVGK----PLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            R+    EIV K    PLT SEKILY HL D   ++   RG  Y+   PDR+ACQDATAQMA+LQFM AG  +VAVP TVHCDHLI+A  G   DL+ AN T+ EV+DFL++   KY IGFW+PG+GIIHQ+VLENYAFPGG++IGTDSHT NAGGLGMVAIGVGGADAVDVMA + WELK PK+IGV+LTG + GWT+PKDVILKVA ILTVKGGTGAIVEYFGPG  +LSCTG GTICNMGAEIGATTS F ++  M  YLRAT+R+ +AD A E    + AD +       ++DQ+I+IDL+ L P LNGPFTPDL+T + +       +N+WP +++ GLIGSCTNSSYED+SRA+SI QQA+DKG+K K+   I PGSEQVR T  RDG L    K G  +  NACGPCIGQW R +D K   KNSIV S+NRNF  R D NP THAFV SP+I  A+  +G ++FNPLTD+L +++G + +F+ PTG  LP KG++     ++AP +      VA+   S+RLQ L PF      ++KG+K+LIKA            GPWL++RGHL NI+NN +IGA+NA N + N +K+QLTGE++ VP+    Y+  GI+ + +G+ N+GEGSSREHAA++PR+LG AAV+ KSFAR
Sbjct:   54 RIKDNLEIVKKRLNRPLTYSEKILYGHL-DDPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDHLIEAYEGGPIDLERANVTNKEVYDFLQTACAKYNIGFWRPGSGIIHQIVLENYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVKLTGQLKGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFPFNPRMSEYLRATNRSAIADYAEEFAPIIAADENA------HYDQIIEIDLNTLEPHLNGPFTPDLATPI-SKFKEAVKKNDWPQELKVGLIGSCTNSSYEDMSRAASICQQAIDKGIKTKSLFTITPGSEQVRATLTRDGQLDTMRKAGGIVLANACGPCIGQWKR-TDVKKGEKNSIVTSYNRNFTGRNDANPATHAFVTSPDIVTAMVFSGDMNFNPLTDTLKDKDGNDFKFEPPTGAGLPSKGYDPGSNTYVAPSSVNVK-DVAIDPHSKRLQRLTPFKKWDGKDMKGLKILIKAKGKCTTDHISAAGPWLKYRGHLQNISNNYMIGAINAENGEANKLKDQLTGEYKTVPNVAIDYRDHGIRWVTLGEQNFGEGSSREHAALEPRYLGGAAVITKSFAR 699          

HSP 2 Score: 234.958 bits (598), Expect = 2.161e-64
Identity = 119/250 (47.60%), Postives = 170/250 (68.00%), Query Frame = 0

Query:  642 FARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKD 880
            F+  ++FNPLTD+L +++G + +F+ PTG  LP KG++     ++AP +      VA+   S+RLQ L PF     + +KG+K+LIKA G           PWL++RGHL NI+NN +IGA+NA N + N +K+QLTGE++ VP+    Y+  GI+ + +G+ N+GEGSSREHAA++PR+LG AAV+ KSFARIHETNLKKQG+L LTF + + YD I   DT +   + +FAP KPLTL +  ADG+ +
Sbjct:  508 FSGDMNFNPLTDTLKDKDGNDFKFEPPTGAGLPSKGYDPGSNTYVAPSSVNVK-DVAIDPHSKRLQRLTPFKKWDGKDMKGLKILIKAKGKCTTDHISAAGPWLKYRGHLQNISNNYMIGAINAENGEANKLKDQLTGEYKTVPNVAIDYRDHGIRWVTLGEQNFGEGSSREHAALEPRYLGGAAVITKSFARIHETNLKKQGLLPLTFADPAAYDKISPFDTVDIDGLTTFAPGKPLTLVVHPADGSAE 756          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|1351858|sp|P49609.1|ACON_GRAGA (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 375/658 (56.99%), Postives = 469/658 (71.28%), Query Frame = 0

Query:    3 NRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +R++  R  + +PLTL+EK+LY HL D  P +  +RG+ ++   P+R+A QDATAQMAL+QFM + + +VAVP+T+HCDHLI A+ GA  D+  A S + EV+DFL S   KYG+GFWKPG+GIIHQ+VLENYAFPG +MIGTDSHT NAGGLG  A+GVGGADAVDVM G+ WELK PK+IGV+LTG +  W +PKDVILKVA ILTVKGGTGAIVEYFG G  +LSCTG GTICNMGAEIGATTS F Y++ M  YL+AT R  +A  A+   E L AD +        +DQ+I+I+LS+L P +NGPFTPDL+  + +    +  +N WP ++  GLIGSCTNSSYED++R++S+ +QAL  G+K K+   I PGSEQVR T  RDGIL  F + G  +  NACGPCIGQW R   PK  P NSI+ SFNRNF++R DGNP THAFVASPEI  A+++AG L FNP TDSL   +G E +   P+G ELP  GF+  +  F  P  D + + V +  DS+RL  LEPF      +   + +LIKA            GPWL+FRGHLDNI+NN LIGAVN  N + N VKN +TGE+  VPDT RAYKA G+K +V+GD NYGEGSSREHAA++PRHLG  AV+VKSFAR  + N
Sbjct:   49 DRLNTVRSKLNRPLTLAEKVLYGHLDD--PERVPVRGETFLKLRPERVALQDATAQMALIQFMASARPQVAVPSTIHCDHLIAAEVGAEEDMAKAKSQNKEVYDFLASAGAKYGLGFWKPGSGIIHQIVLENYAFPGLLMIGTDSHTPNAGGLGACAVGVGGADAVDVMVGLPWELKAPKVIGVKLTGKLQEWASPKDVILKVAGILTVKGGTGAIVEYFGEGVDSLSCTGMGTICNMGAEIGATTSMFPYNSRMGDYLKATGRDGIASLADSFSEQLRADENA------VYDQLIEINLSELEPHINGPFTPDLAHPL-SKFKEEVEKNGWPAELTVGLIGSCTNSSYEDMARSASVVKQALSHGVKSKSIFNITPGSEQVRATISRDGILDTFTEAGGTVLANACGPCIGQWNRSDVPKGTP-NSIITSFNRNFSQRNDGNPQTHAFVASPEIVTAMSLAGSLKFNPATDSLQGADGAEFKLAAPSGDELPVMGFDPGEDTFQPPSDDSTSILVQIDPDSQRLSFLEPFPAWDGKDYTDMPVLIKARGKCTTDHISMAGPWLKFRGHLDNISNNMLIGAVNDENGEINNVKNAVTGEYGTVPDTARAYKAEGVKWVVIGDENYGEGSSREHAALEPRHLGGVAVIVKSFARIHETN 696          

HSP 2 Score: 256.529 bits (654), Expect = 4.006e-72
Identity = 131/253 (51.78%), Postives = 163/253 (64.43%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGT 878
            V   S A  L FNP TDSL   +G E +   P+G ELP  GF+  +  F  P  D + + V +  DS+RL  LEPF     +    + +LIKA G           PWL+FRGHLDNI+NN LIGAVN  N + N VKN +TGE+  VPDT RAYKA G+K +V+GD NYGEGSSREHAA++PRHLG  AV+VKSFARIHETNLKKQGML LTF+N +DYD I   D  +   + +  P +P+T+ +  ADGT
Sbjct:  494 VTAMSLAGSLKFNPATDSLQGADGAEFKLAAPSGDELPVMGFDPGEDTFQPPSDDSTSILVQIDPDSQRLSFLEPFPAWDGKDYTDMPVLIKARGKCTTDHISMAGPWLKFRGHLDNISNNMLIGAVNDENGEINNVKNAVTGEYGTVPDTARAYKAEGVKWVVIGDENYGEGSSREHAALEPRHLGGVAVIVKSFARIHETNLKKQGMLPLTFNNPADYDKIDSSDKVSLVGLKNLTPGEPVTMTVTKADGT 746          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|961512531|sp|D4AT77.1|ACON_ARTBC (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 383/687 (55.75%), Postives = 473/687 (68.85%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKA-----------YGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWE-LPP 675
            MA  +D  R  + +PLT +EKILY+HL D    +   RGK Y+   PDR+ACQDATAQMA+LQFM AG   VA P TVHCDHLI+A+ G   DL  AN  + EV+DFL +   KY IGFWKPG+GIIHQ++LENY FPGG+MIGTDSHT N GGLGM AIGVGGADAVDVMAG+ WELK P +IGV+LTG +SGWTAPKD+ILKVA ILTVKGGTGAI+EY G G  +LSCTG GTICNMGAEIGATTS F ++  M  YL+AT R  + D +    E L  D +        +DQ+I+I+LS+L P +NGPFTPDL+T + +       EN WP +++ GLIGSCTNSSYED+SRA+SIA+ AL+ G+K K+   + PGSEQ+R T ERDG L   E+ G  I  NACGPCIGQW R  D K    NSI+ S+NRNF  R D NP THAFV SP++  A+ IAG L+FNPLTD L +++G E     PTG  LP  G++     + AP AD + + VAV+  S+RLQ+LEPF      + KG+ +LIK             GPWL++RGHLDNI+NN LIGAVNA N + N VKN  TGE+  VP T RAYKA GI  +V+GD NYGEGSSREHAA+QPRHLG  A++ +SFAR  + N     L  +    + F +P  ++ +PP
Sbjct:   44 MAENLDIVRARLNRPLTFAEKILYSHL-DDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPATVHCDHLIEAQLGGDKDLARANEINKEVYDFLSTSCAKYNIGFWKPGSGIIHQILLENYCFPGGLMIGTDSHTPNGGGLGMAAIGVGGADAVDVMAGLPWELKAPNVIGVKLTGQMSGWTAPKDIILKVAGILTVKGGTGAIIEYHGDGVNSLSCTGMGTICNMGAEIGATTSVFPFNDRMYDYLKATKRQSIGDFSRVYAEGLRPDENA------QYDQLIEINLSELEPHINGPFTPDLATPI-SKFKEAVKENNWPSELKVGLIGSCTNSSYEDMSRAASIARDALNHGIKAKSLFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDR-KDVKKNEANSIISSYNRNFTGRNDANPATHAFVTSPDLVVALTIAGTLNFNPLTDKLKDKDGNEFMLAPPTGEGLPANGYDPGRDTYQAPPADRASISVAVSPSSDRLQILEPFKAWDGKDAKGIPILIKCEGKTTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAENGERNSVKNFETGEYDSVPATARAYKARGIPWVVIGDWNYGEGSSREHAALQPRHLGGMAIITRSFARIHETN-----LKKQGMLPLTFADPADYDRIPP 716          

HSP 2 Score: 242.276 bits (617), Expect = 4.185e-67
Identity = 129/258 (50.00%), Postives = 163/258 (63.18%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKA-----------YGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            V+  + A  L+FNPLTD L +++G E     PTG  LP  G++     + AP AD + + VAV+  S+RLQ+LEPF     +  KG+ +LIK             GPWL++RGHLDNI+NN LIGAVNA N + N VKN  TGE+  VP T RAYKA GI  +V+GD NYGEGSSREHAA+QPRHLG  A++ +SFARIHETNLKKQGML LTF + +DYD I      +       A  KP+TL +   DG    +K
Sbjct:  492 VVALTIAGTLNFNPLTDKLKDKDGNEFMLAPPTGEGLPANGYDPGRDTYQAPPADRASISVAVSPSSDRLQILEPFKAWDGKDAKGIPILIKCEGKTTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAENGERNSVKNFETGEYDSVPATARAYKARGIPWVVIGDWNYGEGSSREHAALQPRHLGGMAIITRSFARIHETNLKKQGMLPLTFADPADYDRIPPTAMVDLM-CTELAVGKPMTLRVHPKDGASFDVK 748          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|1168308|sp|P19414.2|ACON_YEAST (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 745.732 bits (1924), Expect = 0.000e+0
Identity = 376/652 (57.67%), Postives = 461/652 (70.71%), Query Frame = 0

Query:    5 VDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEV-KEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            +D  R+ + +P T +EKILY HL D    +   RG  Y+   PDR+ACQDATAQMA+LQFM AG  +VA P TVHCDHLIQA+ G   DLK A   + EV+DFL S + KY +GFWKPG+GIIHQ+VLENYAFPG ++IGTDSHT NAGGLG +AIGVGGADAVDVMAG  WELK PK++GV+LTG ++GWT+PKD+ILK+A I TVKGGTG IVEYFG G    S TG GTICNMGAEIGATTS F ++ SM  YL AT R  +AD A    K+ L+AD D        +D+V++IDL+ L P +NGPFTPDL+T V + M   A  N WP+ V  GLIGSCTNSSYED+SR++SI + A   GLK K    + PGSEQ+R T ERDG L  F++ G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R DGNP THAFVASPE+  A AIAG L FNPLTD L +++G E     P G  LP +G++  +  + AP AD S V+V V+  S+RLQLL+PF P    + K + +LIKA            GPWL++RGHL+NI+NN +IGA+NA NKK N VKN  TGE++GVPDT R Y+  GIK +V+GD N+GEGSSREHAA++PR LG  A++ KSFAR
Sbjct:   48 LDIVRKRLNRPFTYAEKILYGHL-DDPHGQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQVAKPVTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDFLASATAKYNMGFWKPGSGIIHQIVLENYAFPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMAGRPWELKAPKILGVKLTGKMNGWTSPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSATGMGTICNMGAEIGATTSVFPFNKSMIEYLEATGRGKIADFAKLYHKDLLSADKDA------EYDEVVEIDLNTLEPYINGPFTPDLATPV-SKMKEVAVANNWPLDVRVGLIGSCTNSSYEDMSRSASIVKDAAAHGLKSKTIFTVTPGSEQIRATIERDGQLETFKEFGGIVLANACGPCIGQWDR-RDIKKGDKNTIVSSYNRNFTSRNDGNPQTHAFVASPELVTAFAIAGDLRFNPLTDKLKDKDGNEFMLKPPHGDGLPQRGYDAGENTYQAPPADRSTVEVKVSPTSDRLQLLKPFKPWDGKDAKDMPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENKKANCVKNVYTGEYKGVPDTARDYRDQGIKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFAR 690          

HSP 2 Score: 254.218 bits (648), Expect = 2.195e-71
Identity = 136/286 (47.55%), Postives = 181/286 (63.29%), Query Frame = 0

Query:  605 GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFA--RRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADG 877
            G K+ +V  +N    +SR     Q      +  LV +FA    L FNPLTD L +++G E     P G  LP +G++  +  + AP AD S V+V V+  S+RLQLL+PF P   +  K + +LIKA G           PWL++RGHL+NI+NN +IGA+NA NKK N VKN  TGE++GVPDT R Y+  GIK +V+GD N+GEGSSREHAA++PR LG  A++ KSFARIHETNLKKQG+L L F N +DYD I  DD  +   +A  AP KP+T+ +   +G
Sbjct:  460 GDKNTIVSSYNR-NFTSRNDGNPQTHAFVASPELVTAFAIAGDLRFNPLTDKLKDKDGNEFMLKPPHGDGLPQRGYDAGENTYQAPPADRSTVEVKVSPTSDRLQLLKPFKPWDGKDAKDMPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENKKANCVKNVYTGEYKGVPDTARDYRDQGIKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFKNPADYDKINPDDRIDILGLAELAPGKPVTMRVHPKNG 744          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|21264394|sp|P34455.2|ACON_CAEEL (RecName: Full=Probable aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 736.102 bits (1899), Expect = 0.000e+0
Identity = 376/655 (57.40%), Postives = 475/655 (72.52%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            ++  V   ++ + +PLTLSEKILY HL D   ++   RG  Y+   PDR+A QDATAQMA+LQF+ +G  K AVP+T+HCDHLI+A+ G A DL  A   + EVF+FL +  +KYG+GFWKPG+GIIHQ++LENYAFPG ++IGTDSHT N GGLG + IGVGGADAVDVMA + WELK PK+IG++LTG ++GWT+ KDVILKVA+ILTVKGGTGAIVEYFGPG  ++S TG GTICNMGAEIGATTS F Y+ SM +YL AT R ++A+ A + K+ LTAD     +  NY DQ+I+I+L  L P +NGPFTPDL++++   +   A +N WP+ V+  LIGSCTNSSYED++RA+SIA+QALDKGLK K    I PGSEQVR T ERDG+  +F   G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP TH FV SP+IT A+AI+GRLDFNPLTD L   +G + +   PTG +LPPKG++  +  F AP   G   QV V+  S+RLQLL PF      +L+ +K+LIK           A GPWL++RGHLDNI+NN  + A+NA N + N VKNQ+TGE+  VP T R YKA G++ + +GD NYGEGSSREHAA++PRHLG  A++VKSFAR
Sbjct:   45 LSQTVKIVKDRLKRPLTLSEKILYGHL-DQPKTQDIERGVSYLRLRPDRVAMQDATAQMAMLQFISSGLPKTAVPSTIHCDHLIEAQKGGAQDLARAKDLNKEVFNFLATAGSKYGVGFWKPGSGIIHQIILENYAFPGLLLIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGIKLTGKLNGWTSAKDVILKVADILTVKGGTGAIVEYFGPGVDSISATGMGTICNMGAEIGATTSVFPYNESMYKYLEATGRKEIAEEARKYKDLLTAD-----DGANY-DQIIEINLDTLTPHVNGPFTPDLASSI-DKLGENAKKNGWPLDVKVSLIGSCTNSSYEDMTRAASIAKQALDKGLKAKTIFTITPGSEQVRATIERDGLSKIFADFGGMVLANACGPCIGQWDR-QDVKKGEKNTIVTSYNRNFTGRNDANPATHGFVTSPDITTAMAISGRLDFNPLTDELTAADGSKFKLQAPTGLDLPPKGYDPGEDTFQAPSGSG---QVDVSPSSDRLQLLSPFDKWDGKDLEDMKILIKVTGKCTTDHISAAGPWLKYRGHLDNISNNLFLTAINADNGEMNKVKNQVTGEYGAVPATARKYKADGVRWVAIGDENYGEGSSREHAALEPRHLGGRAIIVKSFAR 687          

HSP 2 Score: 257.684 bits (657), Expect = 1.447e-72
Identity = 130/246 (52.85%), Postives = 165/246 (67.07%), Query Frame = 0

Query:  645 RLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTK 879
            RLDFNPLTD L   +G + +   PTG +LPPKG++  +  F AP   G   QV V+  S+RLQLL PF     + L+ +K+LIK           A GPWL++RGHLDNI+NN  + A+NA N + N VKNQ+TGE+  VP T R YKA G++ + +GD NYGEGSSREHAA++PRHLG  A++VKSFARIHETNLKKQGML LTF N +DYD I   D  +   ++SFAP KPLT      +G+K
Sbjct:  501 RLDFNPLTDELTAADGSKFKLQAPTGLDLPPKGYDPGEDTFQAPSGSG---QVDVSPSSDRLQLLSPFDKWDGKDLEDMKILIKVTGKCTTDHISAAGPWLKYRGHLDNISNNLFLTAINADNGEMNKVKNQVTGEYGAVPATARKYKADGVRWVAIGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGMLPLTFANPADYDKIDPSDNVSIVGLSSFAPGKPLTAIFKKTNGSK 743          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|224471814|sp|P82611.2|ACON_CANAL (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 734.947 bits (1896), Expect = 0.000e+0
Identity = 369/657 (56.16%), Postives = 464/657 (70.62%), Query Frame = 0

Query:    5 VDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEV-KEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            V+  +  + +PLT +EK+LY HL D   ++   RG  Y+   PDR+ACQDATAQMA+LQFM AG  +VA P+TVHCDHLIQA+ G   DL  A   + EV+DFL +   KY +GFWKPG+GIIHQ+VLENYAFPG ++IGTDSHT NAGGLG +AIGVGGADAVDVM+G+ WELK PK+IGV+LTG +SGWT+PKD+ILK+A I TVKGGTG+IVEYFG G    SCTG GTICNMGAEIGATTS F ++ SM  YL AT R+++A  A    K+ L+AD          +DQVI+IDL+ L P +NGPFTPDL+T V + M   A  N WP++V+ GLIGSCTNSSYED++RA+SI + A   GLK KA   ++PGSEQVR T  RDG L  FE  G  +  NACGPCIGQW R  D K   KN+IV SFNRNF  R DGNP THAFVASPE+    AI+G L FNP+TD+L+  +G+E +  EP G  LPP G++  +  + AP  D + V+V ++  S+RLQ L PF P    + + + +LIKA            GPWL++RGHL+NI+NN +IGA+NA N K N V+N  TG++ GVP T  AY+ AG K +V+GD N+GEGSSREHAA++PR LG  A++ KSFAR  + N
Sbjct:   49 VEIVKSRLNRPLTYAEKLLYGHL-DDPHNQEIERGVSYLKLRPDRVACQDATAQMAILQFMSAGIPQVATPSTVHCDHLIQAQVGGPKDLARAIDLNKEVYDFLSTACAKYNLGFWKPGSGIIHQIVLENYAFPGALLIGTDSHTPNAGGLGQLAIGVGGADAVDVMSGLPWELKAPKIIGVKLTGKMSGWTSPKDIILKLAGITTVKGGTGSIVEYFGSGVDTFSCTGMGTICNMGAEIGATTSVFPFNDSMVDYLNATGRSEIAQFAQVYKKDFLSADEGA------EYDQVIEIDLNTLEPHINGPFTPDLATPV-SKMKETAIANGWPLEVKVGLIGSCTNSSYEDMTRAASIIKDAGAHGLKSKALYTVSPGSEQVRATIARDGQLKTFEDFGGVVMANACGPCIGQWDR-QDIKKGDKNTIVSSFNRNFTARNDGNPATHAFVASPEMATVYAISGDLGFNPITDTLVGADGKEFKLKEPQGVGLPPDGYDPGENTYQAPPEDRASVEVVISPTSDRLQKLSPFKPWDGKDAERLPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENGKANEVRNHYTGKYDGVPQTAAAYRDAGHKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETN 696          

HSP 2 Score: 234.958 bits (598), Expect = 1.614e-64
Identity = 116/250 (46.40%), Postives = 162/250 (64.80%), Query Frame = 0

Query:  639 VKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADG 877
            V + +  L FNP+TD+L+  +G+E +  EP G  LPP G++  +  + AP  D + V+V ++  S+RLQ L PF P   +  + + +LIKA G           PWL++RGHL+NI+NN +IGA+NA N K N V+N  TG++ GVP T  AY+ AG K +V+GD N+GEGSSREHAA++PR LG  A++ KSFARIHETNLKKQG+L L F N +DYD I  DD  +   + + AP K + L +   +G
Sbjct:  496 VYAISGDLGFNPITDTLVGADGKEFKLKEPQGVGLPPDGYDPGENTYQAPPEDRASVEVVISPTSDRLQKLSPFKPWDGKDAERLPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENGKANEVRNHYTGKYDGVPQTAAAYRDAGHKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFKNPADYDKINFDDEVDLIGLTTLAPGKDVILRVHPKEG 745          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|113159|sp|P16276.1|ACON_PIG (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 729.554 bits (1882), Expect = 0.000e+0
Identity = 370/655 (56.49%), Postives = 469/655 (71.60%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            +   +D  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G   DL+ A   + EV++FL +   KYG+GFW+PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG++SGWT+PKDVILKVA ILTVKGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T RAD+A+ A+E K+HL  D      P  ++DQVI+I+LS+L P +NGPFTPDL+  V    +V   E  WP+ +  GLIGSCTNSSYED+ R++++A+QAL  GLK K++  I PGSEQ+R T ERDG   V   +G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPEI  A+AIAG L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG +V V+  S+RLQLLEPF      +L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N+K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREH A++PRHLG  A++ KSFAR
Sbjct:   48 LEKNIDIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPD------PGCHYDQVIEINLSELKPHINGPFTPDLAHPVAEVGSVAEKEG-WPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENRKANSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGSSREHRALEPRHLGGRAIITKSFAR 693          

HSP 2 Score: 243.047 bits (619), Expect = 2.221e-67
Identity = 126/257 (49.03%), Postives = 164/257 (63.81%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            V   + A  L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG +V V+  S+RLQLLEPF     + L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N+K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREH A++PRHLG  A++ KSFARIHETNLKKQG+L LTF + +DY+ I   D      +  FAP KPL   I H +GT++TI
Sbjct:  496 VTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENRKANSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGSSREHRALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFAPGKPLKCIIKHPNGTQETI 752          

BLAST of aconitate hydratase vs. nr
Match: gi|652763555|ref|WP_027078741.1| (aconitate hydratase [Maribacter antarcticus])

HSP 1 Score: 1251.5 bits (3237), Expect = 0.000e+0
Identity = 603/655 (92.06%), Postives = 629/655 (96.03%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA+RVDKAREIVGKPLTLSEKILY+HLW+GTP+KAF RGKDYVDF PDRIA QDATAQMALLQFMQAGKK+VAVPTTVHCDHLIQAKNGA ADLKFANSTSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAI+LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDR+DVADAANEVKEHLTADA+VYANPENYFDQVIDIDLSKLGPLLNGPFTPDLST VG+DM  KAT+N+WPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMK+ELGINPGSEQVRFTAERDGIL+VFEKL +KIFTNACGPCIGQWARYS+PKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TDSLINENGQEVRFDEPTGWELPP GFEV+DAGFLAPEADGSGVQV VA DSERLQ+LEPF PIQ+ EL+GVKLLIKA+           GPWLRFRGHLDNIANNTLIGAVNA+NKKTNFVKNQ TGEF+GVPDTQRAYKAAGIK+IV+GDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MADRVDKAREIVGKPLTLSEKILYSHLWEGTPTKAFSRGKDYVDFNPDRIALQDATAQMALLQFMQAGKKQVAVPTTVHCDHLIQAKNGATADLKFANSTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAISLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRSDVADAANEVKEHLTADAEVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTPVGSDMTEKATKNDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKSELGINPGSEQVRFTAERDGILNVFEKLDAKIFTNACGPCIGQWARYSNPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPITDSLINENGQEVRFDEPTGWELPPNGFEVQDAGFLAPEADGSGVQVVVAGDSERLQVLEPFLPIQNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNFVKNQATGEFEGVPDTQRAYKAAGIKTIVIGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 446.047 bits (1146), Expect = 7.638e-141
Identity = 213/253 (84.19%), Postives = 229/253 (90.51%), Query Frame = 0

Query:  641 SFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            + A RLDFNP+TDSLINENGQEVRFDEPTGWELPP GFEV+DAGFLAPEADGSGVQV VA DSERLQ+LEPF PIQNE L+GVKLLIKA+G           PWLRFRGHLDNIANNTLIGAVNA+NKKTNFVKNQ TGEF+GVPDTQRAYKAAGIK+IV+GDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLI+EDDTFNF +IA FAPDKPL +E+VHADG+KDT+
Sbjct:  476 AIAGRLDFNPITDSLINENGQEVRFDEPTGWELPPNGFEVQDAGFLAPEADGSGVQVVVAGDSERLQVLEPFLPIQNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNFVKNQATGEFEGVPDTQRAYKAAGIKTIVIGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFAKESDYDLIKEDDTFNFINIADFAPDKPLIIEVVHADGSKDTV 728          

BLAST of aconitate hydratase vs. nr
Match: gi|1274386935|ref|WP_099573898.1| (aconitate hydratase [Maribacter sp. 4U21] >gi|1273375918|gb|PIB27720.1| aconitate hydratase [Maribacter sp. 4U21])

HSP 1 Score: 1232.24 bits (3187), Expect = 0.000e+0
Identity = 594/655 (90.69%), Postives = 619/655 (94.50%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVDKAREIVGKPLTLSEKILY+HLWDG PSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAKNGAAADLKFAN++SAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGA ALSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRADVADAAN+VK++LTAD DVYANPE YFDQVIDIDL++L PLLNGPFTPDLST VG+DM  KAT+N+WPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDK LKMKAELGINPGSEQVRFTAERDGIL +FE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIA+AGRLDFNP+ D+LINENG+EVRFDEPTGWELPPKGFEVEDAGFLAP  DGSGV+V V + SERLQLLEPF PI++ EL+GVKLLIKA+           GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGE+ GVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MAERVDKAREIVGKPLTLSEKILYSHLWDGNPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKNGAAADLKFANTSSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGNISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKALSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANKVKDYLTADEDVYANPEQYFDQVIDIDLTELNPLLNGPFTPDLSTPVGSDMTEKATKNDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKALKMKAELGINPGSEQVRFTAERDGILGIFEDLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAVAGRLDFNPMKDTLINENGEEVRFDEPTGWELPPKGFEVEDAGFLAPMEDGSGVKVVVDDSSERLQLLEPFVPIKNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEYGGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 448.743 bits (1153), Expect = 6.742e-142
Identity = 228/306 (74.51%), Postives = 248/306 (81.05%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  AA+ V   A RLDFNP+ D+LINENG+EVRFDEPTGWELPPKGFEVEDAGFLAP  DGSGV+V V + SERLQLLEPF PI+NE L+GVKLLIKA+G           PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGE+ GVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF NESDYDLIQEDDTFNF DI  FAPDKPLTLEIVHADG+KDTIK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEI-TAAIAV---AGRLDFNPMKDTLINENGEEVRFDEPTGWELPPKGFEVEDAGFLAPMEDGSGVKVVVDDSSERLQLLEPFVPIKNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEYGGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANESDYDLIQEDDTFNFIDIVDFAPDKPLTLEIVHADGSKDTIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1236838942|ref|WP_094995649.1| (aconitate hydratase [Maribacter cobaltidurans] >gi|1236397146|gb|ASV29015.1| aconitate hydratase [Maribacter cobaltidurans])

HSP 1 Score: 1219.53 bits (3154), Expect = 0.000e+0
Identity = 589/655 (89.92%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVDKAR++VGKPLTLSEKILYAHLWDG+PSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK ANS+SAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGA ALSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRADVADAAN+VKE+LTAD +VYANPE YFDQVIDIDLSKL PLLNGPFTPDLST VG +M  KAT+N+WP+QVEWGLIGSCTNSSYEDLSRASSIAQQALDK LK KAE GINPGSEQVR+TAERDGIL +FEKL +KIFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD+LINE GQEVRFDEPTGWELPPKGFEVEDAG+LAP  DGS V+V VA DSERLQLLEPF PI + EL+GVKLLIKAY           GPWLRFRGHLDNIANNTLIGAVNA+NKKTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MAERVDKARDLVGKPLTLSEKILYAHLWDGSPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANSSSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGEISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKALSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANQVKEYLTADDEVYANPEQYFDQVIDIDLSKLNPLLNGPFTPDLSTPVGKEMTKKATDNDWPMQVEWGLIGSCTNSSYEDLSRASSIAQQALDKNLKTKAEFGINPGSEQVRYTAERDGILGIFEKLDAKIFTNACGPCIGQWARYNDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLINEEGQEVRFDEPTGWELPPKGFEVEDAGYLAPREDGSDVKVVVAPDSERLQLLEPFEPITNEELQGVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 444.506 bits (1142), Expect = 2.310e-140
Identity = 214/254 (84.25%), Postives = 227/254 (89.37%), Query Frame = 0

Query:  641 SFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            + A RLDFNP+TD+LINE GQEVRFDEPTGWELPPKGFEVEDAG+LAP  DGS V+V VA DSERLQLLEPF PI NE L+GVKLLIKAYG           PWLRFRGHLDNIANNTLIGAVNA+NKKTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNF DIA FAPDKPLT+E+VHADG+KDTIK
Sbjct:  476 AIAGRLDFNPMTDTLINEEGQEVRFDEPTGWELPPKGFEVEDAGYLAPREDGSDVKVVVAPDSERLQLLEPFEPITNEELQGVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFVDIADFAPDKPLTIEVVHADGSKDTIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1274352022|ref|WP_099543217.1| (aconitate hydratase [Maribacter sp. 4G9] >gi|1273387565|gb|PIB38911.1| aconitate hydratase [Maribacter sp. 4G9])

HSP 1 Score: 1213.36 bits (3138), Expect = 0.000e+0
Identity = 585/655 (89.31%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVDKAREIVGKPLTLSEKILYAHLWDGTP++AF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK ANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRADVADAAN+V+E+LTAD +VYANPE YFDQVIDIDLS L PLLNGPFTPDLST VG +M  KAT+N+WP+QVEWGLIGSCTNSSYEDLSRASSIAQQALDK LK KAE GINPGSEQVR+TAERDGIL +FEKL +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD+LINE+G+EVRF+EPTGWELPPKGFEVEDAG+LAP+ DGS V+V V   SERLQLLEPF PI++ EL+ VKLLIKAY           GPWLRFRGHLDNIANNTLIGAVNA+N+KTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MAERVDKAREIVGKPLTLSEKILYAHLWDGTPTRAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADVADAANKVREYLTADDEVYANPEQYFDQVIDIDLSTLNPLLNGPFTPDLSTPVGKEMTEKATKNDWPMQVEWGLIGSCTNSSYEDLSRASSIAQQALDKKLKTKAEFGINPGSEQVRYTAERDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLINEDGEEVRFEEPTGWELPPKGFEVEDAGYLAPKEDGSSVKVVVDPTSERLQLLEPFEPIKNEELQDVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNQKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 427.172 bits (1097), Expect = 1.188e-133
Identity = 212/306 (69.28%), Postives = 243/306 (79.41%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD+LINE+G+EVRF+EPTGWELPPKGFEVEDAG+LAP+ DGS V+V V   SERLQLLEPF PI+NE L+ VKLLIKAYG           PWLRFRGHLDNIANNTLIGAVNA+N+KTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGML LTF NE+DYDLI+EDDTFNF DI+ FAPD PLT+E+VHADG+KD+IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDTLINEDGEEVRFEEPTGWELPPKGFEVEDAGYLAPKEDGSSVKVVVDPTSERLQLLEPFEPIKNEELQDVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNQKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLALTFANENDYDLIKEDDTFNFVDISDFAPDTPLTIEVVHADGSKDSIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1124238831|ref|WP_074538938.1| (aconitate hydratase [Cellulophaga baltica] >gi|1086759724|emb|SDF23318.1| aconitase [Cellulophaga baltica])

HSP 1 Score: 1212.59 bits (3136), Expect = 0.000e+0
Identity = 582/655 (88.85%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADADVYANPE YFD+VI+I+LS+L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADADVYANPEQYFDEVIEINLSELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDKNLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 426.787 bits (1096), Expect = 1.634e-133
Identity = 214/306 (69.93%), Postives = 240/306 (78.43%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+++ L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDKNLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|639136006|ref|WP_024481492.1| (aconitate hydratase [Cellulophaga baltica])

HSP 1 Score: 1212.21 bits (3135), Expect = 0.000e+0
Identity = 581/655 (88.70%), Postives = 616/655 (94.05%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADA+VYANPE YFD+VI+I+LS+L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+ +KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADAEVYANPEQYFDEVIEINLSELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDESLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGQKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 430.254 bits (1105), Expect = 6.413e-135
Identity = 215/306 (70.26%), Postives = 242/306 (79.08%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E+L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+ +KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDESLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGQKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|657645372|ref|WP_029449842.1| (aconitate hydratase [Cellulophaga baltica])

HSP 1 Score: 1211.05 bits (3132), Expect = 0.000e+0
Identity = 581/655 (88.70%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADA+VYANPE YFD+VI+I+LS+L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADAEVYANPEQYFDEVIEINLSELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 428.713 bits (1101), Expect = 3.433e-134
Identity = 215/306 (70.26%), Postives = 240/306 (78.43%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|646943766|ref|WP_025615707.1| (MULTISPECIES: aconitate hydratase [Cellulophaga] >gi|694993226|gb|KGK31013.1| aconitate hydratase [Cellulophaga sp. E6(2014)] >gi|723620072|gb|AIY13444.1| aconitate hydratase [Cellulophaga baltica NN016038] >gi|730599000|gb|AIZ41795.1| aconitate hydratase [Cellulophaga baltica 18])

HSP 1 Score: 1210.67 bits (3131), Expect = 0.000e+0
Identity = 580/655 (88.55%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADA+VYANPE YFD+VI+I+L++L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADAEVYANPEQYFDEVIEINLTELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 428.713 bits (1101), Expect = 3.255e-134
Identity = 215/306 (70.26%), Postives = 240/306 (78.43%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1219244663|ref|WP_089262032.1| (aconitate hydratase [Maribacter sedimenticola] >gi|1216016929|emb|SNR70143.1| aconitase [Maribacter sedimenticola])

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 577/655 (88.09%), Postives = 610/655 (93.13%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVD+AR IVGKPLTLSEKILY+HLWDGTP+KAF RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GA ADLK ANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAAN+VKEHLTADA+VYANPE YFD+VI+IDL+ L PLLNGPFTPDLST VG+DM  KATE++WP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMKAELGINPGSEQVR+TA+RDGIL +FEKL +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI+GRLDFNP+TD LINE G+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L G KLLIKAY           GPWLRFRGHLDNI+NN LIGAVNA+  KTN VKNQLTGE+ GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MAKRVDEARNIVGKPLTLSEKILYSHLWDGTPTKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGATADLKSANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKSLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADIADAANKVKEHLTADAEVYANPEQYFDEVIEIDLTALTPLLNGPFTPDLSTKVGSDMTKKATEHDWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKAELGINPGSEQVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAISGRLDFNPMTDKLINEKGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVASDSERLQLLEPFTPIKDESLMGAKLLIKAYGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNMVKNQLTGEYGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 422.935 bits (1086), Expect = 4.025e-132
Identity = 211/306 (68.95%), Postives = 239/306 (78.10%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    + RLDFNP+TD LINE G+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E+L G KLLIKAYG           PWLRFRGHLDNI+NN LIGAVNA+  KTN VKNQLTGE+ GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----SGRLDFNPMTDKLINEKGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVASDSERLQLLEPFTPIKDESLMGAKLLIKAYGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNMVKNQLTGEYGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFLDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1134307885|ref|WP_076547862.1| (aconitate hydratase [Maribacter ulvicola] >gi|1131913522|emb|SIQ41371.1| aconitase [Maribacter ulvicola])

HSP 1 Score: 1201.81 bits (3108), Expect = 0.000e+0
Identity = 575/655 (87.79%), Postives = 610/655 (93.13%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M+ RVDKAREIVGKPLTLSEKILY+HLWDG+P++AF RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GA ADLK ANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA  LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEVKEHLTADA+VYANPE YFD+VI+IDL+KL PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMKAELGINPGSEQVR+TA+RDGIL +FEKL +KIFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI+GRLDFNP+TD LIN+ G+EV FDEPTGWELPPKGFEVEDAG+LAP  DGSGV+V VA +SERLQLLEPFTPI+D  L G KLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+ KKTN VKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAV+VKSFAR
Sbjct:   15 MSERVDKAREIVGKPLTLSEKILYSHLWDGSPTEAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGATADLKSANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGDISGWTAPKDVILKVAEILTVKGGTGAIVEYFGEGAKNLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADIADAANEVKEHLTADAEVYANPEQYFDEVIEIDLAKLTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKAELGINPGSEQVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYNDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAISGRLDFNPMTDKLINQKGEEVMFDEPTGWELPPKGFEVEDAGYLAPRKDGSGVEVKVASNSERLQLLEPFTPIKDESLMGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNMVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFAR 669          

HSP 2 Score: 427.172 bits (1097), Expect = 1.321e-133
Identity = 202/250 (80.80%), Postives = 222/250 (88.80%), Query Frame = 0

Query:  645 RLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            RLDFNP+TD LIN+ G+EV FDEPTGWELPPKGFEVEDAG+LAP  DGSGV+V VA +SERLQLLEPFTPI++E+L G KLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+ KKTN VKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAV+VKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNFTDIA FAPDKPLT+EIVH+DG+KD IK
Sbjct:  480 RLDFNPMTDKLINQKGEEVMFDEPTGWELPPKGFEVEDAGYLAPRKDGSGVEVKVASNSERLQLLEPFTPIKDESLMGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNMVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFTDIAEFAPDKPLTVEIVHSDGSKDVIK 729