BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000008807 (supercontig:LSalAtl2s:LSalAtl2s549:112963:178758:1 gene:EMLSAG00000008807 transcript:EMLSAT00000008807 description:"augustus_masked-LSalAtl2s549-processed-gene-1.0")

HSP 1 Score: 443.351 bits (1139), Expect = 8.945e-152
Identity = 212/246 (86.18%), Postives = 228/246 (92.68%), Query Frame = 0

Query:  179 MIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENR 424
            MIACYKGH +IAKYLI IGADVNRKSVKGNTALHDCAESGSLEIMKLLL +GA MDVDAYGMTPLLAASVTGH+HIVE+LIS+  LV+R+ RIDALELLGATFVDKKRDM+GSYKLWK+AMEER +  +LV PK V+ASPISAYENTVEV +++QL +IISDPD MRMQALLVRERILGPAHPDTSYYIRYRGAVYADMG FERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMM E R
Sbjct:    1 MIACYKGHLRIAKYLIAIGADVNRKSVKGNTALHDCAESGSLEIMKLLLDHGAIMDVDAYGMTPLLAASVTGHIHIVEYLISEPNLVSRQERIDALELLGATFVDKKRDMVGSYKLWKRAMEERYIGNQLVVPKCVVASPISAYENTVEVETIEQLEEIISDPDEMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGLFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMKETR 246          

HSP 2 Score: 90.1225 bits (222), Expect = 1.560e-19
Identity = 44/83 (53.01%), Postives = 56/83 (67.47%), Query Frame = 0

Query:  436 NFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRG 518
            NFCDIMTVFNKAV++V MG  ++     + V+ +RDITYFNR +IIILHL+CL TKL+ HL   QI +VK       K   +G
Sbjct:  312 NFCDIMTVFNKAVKQVEMGLESM-----DLVNLERDITYFNRAIIIILHLVCLFTKLLSHLKESQIFDVKKGSLQLFKTRSKG 389          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000008998 (supercontig:LSalAtl2s:LSalAtl2s564:197051:204371:1 gene:EMLSAG00000008998 transcript:EMLSAT00000008998 description:"maker-LSalAtl2s564-augustus-gene-2.12")

HSP 1 Score: 304.294 bits (778), Expect = 1.706e-94
Identity = 202/621 (32.53%), Postives = 325/621 (52.33%), Query Frame = 0

Query:   53 KDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTF-DGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDA-YGMTPLLAASVTGHMHIVEFLISKTELVTRKSRI-DALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSF------AELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFL-KLHPRGRNGNTPLHLACARDSS-------SVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            K   +++ S   +G +PL +AC+ G+ ++VEYL+  C A++EQ G     D  ++    PLWCAA AG   +V  L   GANVN  + T STP+R+ACF  H EIV+ L+   ADI+  N +G TCL+ +      ++ ++L++ GADVN + ++  TALH   +       KLLL  GA   + + YG   L  A + G   I ++L+    L     R+ +  ELLG+TF+D+  D   + + WK A + R    K    K +  +  + Y N  E     +L  +  D DAMRMQ+LL+ ER+LG  H DT Y I YRGA YAD   ++ CI LW YAL+++         +T+ S+L F        L   ++  +   S     + V   D++++    +++V +    +K  P      D      ++ L  I +L+ L+T+L P+       E++  ++H +  ++P   +G+T LHL  +  +          G Y    FP+L+ +++LVECGA+ NA ++ ++T LH+AA  +  +  +++ LL  G H+D  +  G T   LLK      ++NP+KYT L+CLAAQVI R  + ++  +   LE F+  H
Sbjct:   56 KSTRREITSKVKDGTSPLFIACKKGNVEIVEYLVTTCSADIEQKGVYEVQDDNSVHKVTPLWCAAVAGKVKVVEXLVNHGANVNCESDTGSTPVRSACFMTHLEIVKILVLNNADIQKPNYNGGTCLINSVQS--VELCEFLLKHGADVNAQDIQCKTALHYAIQEHRSATTKLLLQYGAIPFLKSRYGDDALQTACLKGAWEIFDYLVD--HLPYSPERVANCFELLGSTFLDEHHDSQKTIQYWKAACDIR---EKFGLKKPLTQNAKNNYTNMREFVDWTELESVALDLDAMRMQSLLICERVLGSLHKDTIYRIIYRGAAYADSLQYQYCIDLWKYALELR---------ITKDSILFFDTCYTAQALVKLLLDVHEKNSQGIIPTQVRLKDVVSIIELLIKDVALSSGLLKVAPVYIRQRDS----HDQILCTITNLLYLVTQL-PNGEIMLSLELRKKIHHLVHNVNPTTADGSTLLHLTVSESNVLKTQNIIEEGHYSF--FPNLEVLKILVECGANVNAMNSTASTPLHIAAVPQNYRTKIIELLLKNGAHIDIRDFYGVTPCRLLKDIG-SCLVNPLKYTTLKCLAAQVISRHNLPFQRQVPAALEAFIDAH 652          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000004250 (supercontig:LSalAtl2s:LSalAtl2s222:596314:649196:-1 gene:EMLSAG00000004250 transcript:EMLSAT00000004250 description:"maker-LSalAtl2s222-augustus-gene-6.21")

HSP 1 Score: 253.447 bits (646), Expect = 4.026e-72
Identity = 184/569 (32.34%), Postives = 298/569 (52.37%), Query Frame = 0

Query:   38 DGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIE-GAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACF-------DGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGAR-MDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRI-DALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELF-----SFMMSENRNRSNPNDRS--PVNFCDIM--TVF-NKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRN-GNTPLHLACARDSSSVGRYPICQ-----FPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPA 580
            D   ++++VF     K  V Q       G TPL LAC+NG  +VVEYL+  C A++EQ G      E +     PLWCAA +G  S+VR+L + GA+VN+ + + STP+R+AC+         HF+I+Q L++ GA+I+  N  G TCL+ +      K+ K+LI+   D+N + V+  TALH   +       KLL+ NGA  M V  YG   L  A + G++ +  +LI K +      RI +A EL+G+TF+    D+  +   WK+A+E R   G   YPK+ +   +       E+ SL++L   ++D   ++ QAL++ ERILG  H DT +   Y GA +AD+  +  C+ LW YAL ++ +    LS  +  +  +  +L+     S   S+N N S    R    V+F D++  T+F N+ +  V+  QL +K  P   V  D      +  L   +HLI +L K+   +S +++ ++ + V   L + P+  N G++  HLA +  S+      + +     FPSL+  + +++C  D + ++N   T LH+A+  KPA
Sbjct:   24 DSLKKKIEVFDKSDRKGVVDQ---TSEFGCTPLFLACKNGSAEVVEYLLTECEADIEQRGIFQVLDEGVSHSVTPLWCAAVSGRLSVVRVLIDNGADVNAASDSGSTPVRSACYIVRNEMPTSHFDIIQALVKAGANIQTPNHFGGTCLINSIQST--KLVKFLIDNDVDINAEDVQQKTALHYAVQENRFATAKLLMQNGADPMKVSKYGDDILQTACLKGNIMVFNYLIDKVKY--DPYRIANAFELIGSTFLLDLYDLGSTLFFWKRALEIR-YSGVASYPKEDLK--VHPNLKVSEINSLEELDSSLTDLQGLKFQALIMSERILGEDHKDTIFRYMYCGASHADVNQYNECVHLWNYALKLKIRKDTLLSYDSSFTARAIVQLYVNILRSVFRSDNENMSMSEIREECEVSFKDVLSTTIFINEGL--VKALQL-LKVNPVFQVQLDN----LDIILTSWIHLIHILLKMA--ISEEEMRQIYLIVKEVLSIDPKTSNGGDSVFHLAVSSSSTLKSNSFLDEENAPLFPSLELTKFILKCDFDVHKRNNLGETPLHIAS--KPA 571          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000002720 (supercontig:LSalAtl2s:LSalAtl2s1574:38056:40533:-1 gene:EMLSAG00000002720 transcript:EMLSAT00000002720 description:"maker-LSalAtl2s1574-snap-gene-0.35")

HSP 1 Score: 148.288 bits (373), Expect = 8.818e-39
Identity = 128/457 (28.01%), Postives = 218/457 (47.70%), Query Frame = 0

Query:  209 TALHDCAESGSLEIMKLLLSNGARMDVDA-----------YGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKR--DMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDS--SSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSL-HVAAQNKPAKPLL-----VQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYT-KLQCLAAQVIRREGINYRG 643
            T L   A+ G+L+++ +LL+  + ++++A            G T L  A+ +GH  IV  LI K                 A+F + K   D L S    ++A+E R  +  L   K+ ++ PISAY N  E+ S  +L  + +D   ++M+ L++RERILG ++ D  + I YRGA+ AD    ++CI LW++AL +++   E +S     +L  F +LF  M+  N            +  D   V      E+ +G    +   AN              + I+++L+ LL  +   ++ +Q      +++  +K+ PR   G + LHLA       +S+  + + Q PS    ++L++ G++P  KDN+ NT L H+A   +     L     + +LL  G H+DAVN  GKT   L       + I   KY  +L+CLAAQ I+++ + + G
Sbjct:   49 TLLMVAAKLGNLDLINMLLTYHS-LNLEAEGNVIFEGSLILGATALWVAAASGHGTIVTALIRK-----------------ASFANDKECYDHLKSLHYIRRALELRESENIL---KENLSEPISAYGNFSEIQSSSELRVMQNDEYRLQMEGLIMRERILGTSNLDVCHPIIYRGAMLADGFMLDKCILLWLHALRIEKSNKEGIS----MNLFHFVQLFCQMIRFNL---------KFDLADFQEVLEACAXEIELGTSLDQSVXAN--------------MKIMVYLMILLNHIESSITEEQYFIYMKTIHRLIKIEPRTECGGSLLHLAADGKHLVTSLNTFNLIQLPSSSVTKMLLKAGSNPEIKDNNGNTPLHHIAEYERHVSDFLTIYNIITSLLFHGAHIDAVNKLGKT--PLDNSSITAKAILREKYKLRLKCLAAQSIQKQKLPFLG 455          

HSP 2 Score: 59.6918 bits (143), Expect = 1.408e-9
Identity = 40/137 (29.20%), Postives = 69/137 (50.36%), Query Frame = 0

Query:   30 NVVFNAARDGKLRRLKVFLDHRAKDEVKQLVS--AKTN----GATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYL 160
            ++ F AA++G   RL   L+    ++ ++L+   + TN      T L++A + G+ D++  L+     N+E  G+V F+G  I GA  LW AAA+GH +IV  L  + +  N             C+D H + + Y+
Sbjct:    5 DLYFFAAQEGCTERLWTLLEETNPEDRRKLLETLSSTNLNDEKCTLLMVAAKLGNLDLINMLLTYHSLNLEAEGNVIFEGSLILGATALWVAAASGHGTIVTALIRKASFAND----------KECYD-HLKSLHYI 130          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000003838 (supercontig:LSalAtl2s:LSalAtl2s2086:4561:15986:1 gene:EMLSAG00000003838 transcript:EMLSAT00000003838 description:"maker-LSalAtl2s2086-augustus-gene-0.4")

HSP 1 Score: 120.553 bits (301), Expect = 2.775e-28
Identity = 87/247 (35.22%), Postives = 129/247 (52.23%), Query Frame = 0

Query:   24 HKM-EFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTK-TNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLS-NGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTR 267
            HK  E    +  A+ DG ++  ++ LD  A     Q+     +  +PL LA   GH ++   L+ER  AN+E+           EG  PL  A+  GH  +V LL  QGA++N+ T+ T  T L  AC  G  ++  +LI+ GADIE+      T LM A  +GH ++ ++L+E  A VN ++  G+TAL    E+G  ++  LLLS NG        G TPL+ A+  GH+  V+FLISK   + R
Sbjct:  529 HKTDEMHTALMEASMDGHVQVARLLLDSGA-----QVNMPADSFESPLTLAACGGHVELAMLLLERG-ANIEEVND--------EGYTPLMEASREGHEEMVALLLSQGADINAQTEETQETALTLACCGGFLDVADFLIKAGADIELG---ASTPLMEAAQEGHLELVRHLLENHAHVNAQTNTGDTALTYTCENGHTDVADLLLSYNGELEHESEGGRTPLMKAARAGHLCTVQFLISKGADINR 758          

HSP 2 Score: 106.686 bits (265), Expect = 7.009e-24
Identity = 83/257 (32.30%), Postives = 126/257 (49.03%), Query Frame = 0

Query:   35 AARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQ-------PGSVTFDGETIE--------------------GAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTN-STPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKG--NTALHDCAESGSLEIMKLLLSNGARMDV-DAYGMTPLLAASVTGHMHIVEFLIS 260
            AA  G  + +++ LDH    E++    +     TPL LAC  G  +VVE L+ R +AN E        P S+   G  +                     G  PL  AA  GH++ V+LL + G+++N+  +TN +T L  ACF G  E+V  L++R A +E   + G T LM A   G+    + L++ GADVN   V    +TAL   A+ G    ++LL+  GA++DV +  G + L  AS  GH+ + + L S
Sbjct: 1612 AATAGHEKVVEILLDHTVAAEIE--AQSDRTKDTPLSLACSGGRYEVVEILLSR-HANKEHRNVSDYTPLSLAASGGYVNIIKLLLSHGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLLDRKAAVEHRAKTGLTPLMEAASGGYVXXGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYRFVELLVFRGAQVDVRNKKGNSSLWLASNGGHLDVXQLLHS 1865          

HSP 3 Score: 105.145 bits (261), Expect = 2.162e-23
Identity = 79/269 (29.37%), Postives = 131/269 (48.70%), Query Frame = 0

Query:   32 VFNAARDGKLRRLKVFLDH---RAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIV-RLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLM----------------------------------IACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGM-TPLLAASVTGHMHIVEFLISK 261
            +  A  +G + +++  LD       D  +Q       G + L LAC  G+ ++ + L+    ANVE  G      E      PL  AA AGH  IV  LL   GA+VN+ + + +TPL  AC  GH ++V  L++ GA++E  N +GHT LM                                  +ACYKGH ++ ++L++ GAD   K+ + +TAL + +  G +++ +LLL +GA++++ A    +PL  A+  GH+ +   L+ +
Sbjct:  336 LVEACNEGDISKVRKLLDEGRVSVNDPTEQ-------GESLLSLACSAGYFELAQVLLA-MGANVEDRG---MKNECT----PLMEAATAGHVDIVTXLLLPHGADVNAQSSSGNTPLMYACAGGHEDVVLTLLKGGANVEDHNENGHTPLMEAASAGHVGVAKILLKYGAGINTHSNEFKESALTLACYKGHLEMVRFLLDAGADQEHKTDEMHTALMEASMDGHVQVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAMLLLER 589          

HSP 4 Score: 100.138 bits (248), Expect = 6.817e-22
Identity = 66/191 (34.55%), Postives = 101/191 (52.88%), Query Frame = 0

Query:   70 LVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGN-TALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLI 259
            L LAC  GH ++V +L++   A  +Q      + +T E    L  A+  GH  + RLL + GA VN    +  +PL  A   GH E+   L+ERGA+IE  N  G+T LM A  +GH ++   L+  GAD+N ++ +   TAL      G L++   L+  GA  D++    TPL+ A+  GH+ +V  L+
Sbjct:  505 LTLACYKGHLEMVRFLLD---AGADQ------EHKTDEMHTALMEASMDGHVQVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAMLLLERGANIEEVNDEGYTPLMEASREGHEEMVALLLSQGADINAQTEETQETALTLACCGGFLDVADFLIKAGA--DIELGASTPLMEAAQEGHLELVRHLL 684          

HSP 5 Score: 91.2781 bits (225), Expect = 4.588e-19
Identity = 72/236 (30.51%), Postives = 111/236 (47.03%), Query Frame = 0

Query:   32 VFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGA--TPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGN-TALHDCAESGSLEIMKLLLSNGARMDVDAY-----GMTPLLAASVTGHMHIVEFLI 259
            +  A+R+G    + + L   A       ++A+T     T L LAC  G  DV ++LI +  A++E           +  + PL  AA  GH  +VR L E  A+VN+ T T  T L   C +GH ++   L+    ++E  +  G T LM A   GH    ++LI  GAD+NR +   + T L      G L +++LLL  GA    D Y       T ++ A+  G+  +V+ LI
Sbjct:  604 LMEASREGHEEMVALLLSQGAD------INAQTEETQETALTLACCGGFLDVADFLI-KAGADIE-----------LGASTPLMEAAQEGHLELVRHLLENHAHVNAQTNTGDTALTYTCENGHTDVADLLLSYNGELEHESEGGRTPLMKAARAGHLCTVQFLISKGADINRPTTNNDHTPLSLACAGGHLSVVELLLQAGA----DPYHKLRDNSTMIIEAAKGGYTSVVQLLI 817          

HSP 6 Score: 88.1965 bits (217), Expect = 3.634e-18
Identity = 69/226 (30.53%), Postives = 112/226 (49.56%), Query Frame = 0

Query:   61 SAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQ--GANVNSTT-KTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRK--SVKGNTALHDCAESGSLEIMKLLLSNGARMD--VDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGA 279
            + ++N  T L LAC  GH+D+V+ L+ R  AN+E            +G  PL  AA AGH  +V +L +    A + + + +T  TPL  AC  G +E+V+ L+ R A+ E  N   +T L +A   G+  I K L+  GA++N +  S  G + L   A +G    +KLLL  G+ ++  ++    T L  A   G   +V  L+ +   V  +++     L+ A
Sbjct: 1567 ATESNHDTALTLACAGGHEDLVKLLLSRG-ANIEH--------RDKKGFTPLILAATAGHEKVVEILLDHTVAAEIEAQSDRTKDTPLSLACSGGRYEVVEILLSRHANKEHRNVSDYTPLSLAASGGYVNIIKLLLSHGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLLDRKAAVEHRAKTGLTPLMEA 1783          

HSP 7 Score: 79.337 bits (194), Expect = 2.204e-15
Identity = 56/166 (33.73%), Postives = 84/166 (50.60%), Query Frame = 0

Query:   32 VFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTN-GATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVN-STTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIE 195
            +  AA++G L  ++  L++ A       V+A+TN G T L   C NGH DV + L+     N E       + E+  G  PL  AA AGH   V+ L  +GA++N  TT  + TPL  AC  GH  +V+ L++ GAD     R   T ++ A   G+  + + LI+
Sbjct:  668 LMEAAQEGHLELVRHLLENHAH------VNAQTNTGDTALTYTCENGHTDVADLLLSY---NGE------LEHESEGGRTPLMKAARAGHLCTVQFLISKGADINRPTTNNDHTPLSLACAGGHLSVVELLLQAGADPYHKLRDNSTMIIEAAKGGYTSVVQLLID 818          

HSP 8 Score: 73.1738 bits (178), Expect = 1.705e-13
Identity = 58/204 (28.43%), Postives = 99/204 (48.53%), Query Frame = 0

Query:   35 AARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNS--TTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVD 236
            AA +G    +K+ LD    D   Q+   +TN  T L LAC  G  +VV  L++R  A VE             G  PL  AA+ G+    R+L ++GA+VN+     +  T L  A   GH+  V+ L+ RGA ++V N+ G++ L +A   GH  + + L    A+++ +  +  + L      G  +++K ++ + ++   D
Sbjct: 1716 AAMNGHTAAVKLLLD-MGSDINAQI---ETNRNTALTLACFQGRHEVVSLLLDR-KAAVEHRAKT--------GLTPLMEAASGGYVXXGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYRFVELLVFRGAQVDVRNKKGNSSLWLASNGGHLDVXQLLHSARANIDSQDNRKVSCLMASFRKGHSKVVKWMVKHVSQFPSD 1906          

HSP 9 Score: 67.3958 bits (163), Expect = 1.172e-11
Identity = 44/135 (32.59%), Postives = 80/135 (59.26%), Query Frame = 0

Query:  133 NVNSTTKTN-STPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIE--IGADVNRKSVK-GNTALHDCAESGSLEIMKLLLS---NGARMDVDAYGMTPLLAASVTGHMHIVEFLIS 260
            +++S T++N  T L  AC  GH ++V+ L+ RGA+IE  ++ G T L++A   GH K+ + L++  + A++  +S +  +T L      G  E++++LLS   N    +V  Y  TPL  A+  G+++I++ L+S
Sbjct: 1563 DIDSATESNHDTALTLACAGGHEDLVKLLLSRGANIEHRDKKGFTPLILAATAGHEKVVEILLDHTVAAEIEAQSDRTKDTPLSLACSGGRYEVVEILLSRHANKEHRNVSDY--TPLSLAASGGYVNIIKLLLS 1695          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000012208 (supercontig:LSalAtl2s:LSalAtl2s884:7796:22423:1 gene:EMLSAG00000012208 transcript:EMLSAT00000012208 description:"maker-LSalAtl2s884-snap-gene-0.6")

HSP 1 Score: 111.694 bits (278), Expect = 1.603e-25
Identity = 72/224 (32.14%), Postives = 106/224 (47.32%), Query Frame = 0

Query:   39 GKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLISK 261
            G+ + L+ FL+      V+       NG T L  A   G ++  E LI R        G        + G+PPL  A   GH ++   L  +GA++     T  +PL  AC +GH  +V+  + RGAD+  A+R G TC+  AC KG  +   YLI+ G D++     G T L   +  G+ EI+ LLL  GA M+  D  GM PL  A    +  +V+  + K
Sbjct: 1065 GRTQALEFFLNMAX---VRVNFHETLNGETGLCAAASAGQQECCEILIRR--------GGCALTA-NLSGSPPLHVAIKNGHWNVTDFLLREGAHIEQVDSTGKSPLIIACMEGHLGLVELFVSRGADMTRADRDGITCIGWACLKGQCEAVSYLIDQGCDISVADNTGRTPLDLASFKGNSEIVTLLLEKGASMEHTDINGMRPLDRAISCRNASVVQCFLKK 1276          

HSP 2 Score: 92.4337 bits (228), Expect = 1.716e-19
Identity = 56/174 (32.18%), Postives = 89/174 (51.15%), Query Frame = 0

Query:   60 VSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARM 233
            ++A  +G+ PL +A +NGH +V ++L+ R  A++EQ  S         G  PL  A   GH  +V L   +GA++    +   T +  AC  G  E V YLI++G DI VA+  G T L +A +KG+ +I   L+E GA +    + G   L       +  +++  L  GAR+
Sbjct: 1116 LTANLSGSPPLHVAIKNGHWNVTDFLL-REGAHIEQVDST--------GKSPLIIACMEGHLGLVELFVSRGADMTRADRDGITCIGWACLKGQCEAVSYLIDQGCDISVADNTGRTPLDLASFKGNSEIVTLLLEKGASMEHTDINGMRPLDRAISCRNASVVQCFLKKGARL 1280          

HSP 3 Score: 70.8626 bits (172), Expect = 8.935e-13
Identity = 60/211 (28.44%), Postives = 89/211 (42.18%), Query Frame = 0

Query:  108 GAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIE------------------------RG-----------ADIEVANRH----GHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLG 278
            G  PL  A+  G+  IVR L E GA VN T + +   L  A   GH  +++ LIE                        RG           A + V N H    G T L  A   G  +  + LI  G      ++ G+  LH   ++G   +   LL  GA ++ VD+ G +PL+ A + GH+ +VE  +S+   +TR  R D +  +G
Sbjct:  985 GVTPLMFASMKGYLDIVRFLLESGATVNKTDRNDRCALVYAAQHGHLAVIEVLIEYDWDNDHDLXLAEAAQQAMVMAASRGRTQALEFFLNMAXVRV-NFHETLNGETGLCAAASAGQQECCEILIRRGGCALTANLSGSPPLHVAIKNGHWNVTDFLLREGAHIEQVDSTGKSPLIIACMEGHLGLVELFVSRGADMTRADR-DGITCIG 1193          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000001760 (supercontig:LSalAtl2s:LSalAtl2s12:1757316:1795906:1 gene:EMLSAG00000001760 transcript:EMLSAT00000001760 description:"maker-LSalAtl2s12-snap-gene-18.22")

HSP 1 Score: 99.7525 bits (247), Expect = 8.446e-22
Identity = 76/253 (30.04%), Postives = 126/253 (49.80%), Query Frame = 0

Query:   68 TPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLIS-------KTELVT------RKSRIDALELL---GATFVDKKRDMLGSYKLWKQAMEERI 303
            TPL +A + G  ++V  L+    AN+E          T +G  PL CAA +GH ++V L+ ++GA +++ TK    PL  +    H +  + LI   A ++       T L +A + GH ++AK L++  AD N +++ G T LH   +   +++++LLL  GA ++     G+TPL  AS  G M+IV +L+         TEL T      R   ID + LL   GA    K +D+  +  +  +  +E +
Sbjct:  247 TPLHVAAKWGKTNMVSLLLNNG-ANLE--------ARTRDGLTPLHCAARSGHENVVELMIQKGAPISAKTKNGLAPLHMSSQGDHVDAARVLIHYKAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDCKADPNSRALNGFTPLHIACKKNRIKLVELLLKYGASIESTTESGLTPLHVASFMGCMNIVIYLLQHQAGPDDATELQTPLHIASRLGNIDIVVLLLQSGAQIDSKTKDLYTALHIAAKEGQEEV 490          

HSP 2 Score: 96.6709 bits (239), Expect = 7.337e-21
Identity = 75/228 (32.89%), Postives = 116/228 (50.88%), Query Frame = 0

Query:   35 AARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDA-YGMTPLLAASVTGHMHIVEFLISK 261
            AA++G+     V LDH A        +    G TPL LA + G+K+V + L+++          V  +G+   G  PL  A+   H ++  LL +  A+ ++  K   TPL  +      EI Q L+E GAD    ++ G T L +A  +GH + +  LI  GA+VN  +  G T LH CA+  S+E+ ++L S GA M+     G +PL  AS  G  ++V+FL+ K
Sbjct:  482 AAKEGQEEVASVLLDHSASR-----TAPTKKGFTPLHLAAKYGNKNVCQLLLQK-------QAPVNCEGKN--GVTPLHVASHYDHPNVAILLLDNNASPHAVAKNGYTPLHISSKKNQLEITQSLLEFGADTNAMSKAGFTPLHLASQEGHKETSNKLIANGANVNAAAKNGLTPLHLCAQEDSIEVAEMLTSLGADMEAQTKQGYSPLHVASHFGSTNMVKFLLEK 695          

HSP 3 Score: 80.4925 bits (197), Expect = 8.674e-16
Identity = 70/251 (27.89%), Postives = 113/251 (45.02%), Query Frame = 0

Query:   62 AKTNGATPLVLACRNGHKDVVEYLIE---RCYA------------NVEQPGSVTF-----DGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLI--------------SKTELVTRKSRIDALELL 277
            A  +G TPL +A + GH  VV  L+E   R  A            +V+  G +       D  +  G  PL  +A  G+  +  LL ++ ANVN   K N TPL  A   G   +V  L+  GA++E   R G T L  A   GH  + + +I+ GA ++ K+  G   LH  ++   ++  ++L+   A +D V    +T L  A+  GH+ + + L+              +   +  +K+RI  +ELL
Sbjct:  146 ATEDGFTPLAVALQQGHDQVVTLLLESDSRGKARLPALHIAAKKDDVKAAGLLLHNDHNPDVTSKSGFTPLHISAHYGNEGVALLLLQKNANVNFVAKHNITPLHVAAKWGKTNMVSLLLNNGANLEARTRDGLTPLHCAARSGHENVVELMIQKGAPISAKTKNGLAPLHMSSQGDHVDAARVLIHYKAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDCKADPNSRALNGFTPLHIACKKNRIKLVELL 396          

HSP 4 Score: 79.7221 bits (195), Expect = 1.568e-15
Identity = 68/226 (30.09%), Postives = 108/226 (47.79%), Query Frame = 0

Query:   35 AARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAY-GMTPLLAASVTGHMHIVEFLI 259
            AA  G +R  K+ LD +A    + L     NG TPL +AC+     +VE L++            + +  T  G  PL  A+  G  +IV  L +  A  +  T+   TPL  A   G+ +IV  L++ GA I+   +  +T L IA  +G  ++A  L++  A     + KG T LH  A+ G+  + +LLL   A ++ +   G+TPL  AS   H ++   L+
Sbjct:  351 AAHCGHVRVAKLLLDCKADPNSRAL-----NGFTPLHIACKKNRIKLVELLLKY---------GASIESTTESGLTPLHVASFMGCMNIVIYLLQHQAGPDDATEL-QTPLHIASRLGNIDIVVLLLQSGAQIDSKTKDLYTALHIAAKEGQEEVASVLLDHSASRTAPTKKGFTPLHLAAKYGNKNVCQLLLQKQAPVNCEGKNGVTPLHVASHYDHPNVAILLL 561          

HSP 5 Score: 78.5666 bits (192), Expect = 3.278e-15
Identity = 62/195 (31.79%), Postives = 98/195 (50.26%), Query Frame = 0

Query:   65 NGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFL 258
            NG TPL ++ +    ++ + L+E         G+ T +  +  G  PL  A+  GH      L   GANVN+  K   TPL     +   E+ + L   GAD+E   + G++ L +A + G   + K+L+E   D N ++  G TALH  ++ G  +I+ +LLS+GA  + V   G TPL  A   G++ +VE L
Sbjct:  573 NGYTPLHISSKKNQLEITQSLLEF--------GADT-NAMSKAGFTPLHLASQEGHKETSNKLIANGANVNAAAKNGLTPLHLCAQEDSIEVAEMLTSLGADMEAQTKQGYSPLHVASHFGSTNMVKFLLEKKVDPNVQNGIGYTALHQASQQGHPQIVNMLLSHGASPNSVSNNGQTPLSIAQRLGYISVVETL 758          

HSP 6 Score: 77.0258 bits (188), Expect = 1.025e-14
Identity = 54/163 (33.13%), Postives = 79/163 (48.47%), Query Frame = 0

Query:  115 AAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAY-GMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALEL 276
            AA  G+   V    +Q  N+NS        L  A  +GH EIV  L+ RG+ ++ A R G+T L IA   G   I K L++  A VN +S  G   L+  A+   ++ + LLL++GA   +    G TPL  A   GH  +V  L+        K+R+ AL +
Sbjct:   25 AARGGNLEKVLEYLKQSTNINSCNANGLNALHLASKEGHVEIVSELLSRGSIVDAATRKGNTALHIASLAGQDAIVKMLVDHDATVNLQSQNGFXPLYMAAQENHVQAVHLLLASGANQSLATEDGFTPLAVALQQGHDQVVTLLLESDS--RGKARLPALHI 185          

HSP 7 Score: 70.0922 bits (170), Expect = 1.196e-12
Identity = 58/193 (30.05%), Postives = 90/193 (46.63%), Query Frame = 0

Query:   68 TPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLI 259
            TPL +A R G+ D+V  L+        Q G+   D +T +    L  AA  G   +  +L +  A+  + TK   TPL  A   G+  + Q L+++ A +    ++G T L +A +  H  +A  L++  A  +  +  G T LH  ++   LEI + LL  GA  + +   G TPL  AS  GH      LI
Sbjct:  444 TPLHIASRLGNIDIVVLLL--------QSGA-QIDSKTKDLYTALHIAAKEGQEEVASVLLDHSASRTAPTKKGFTPLHLAAKYGNKNVCQLLLQKQAPVNCEGKNGVTPLHVASHYDHPNVAILLLDNNASPHAVAKNGYTPLHISSKKNQLEITQSLLEFGADTNAMSKAGFTPLHLASQEGHKETSNKLI 627          

HSP 8 Score: 55.0694 bits (131), Expect = 5.617e-8
Identity = 47/189 (24.87%), Postives = 84/189 (44.44%), Query Frame = 0

Query:   50 HRAKDEVKQLVSAKTNGATPLVLACRNGH-KDVVEYL-----IERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGAR 232
            H   ++    ++ K +     + A R G+ + V+EYL     I  C AN               G   L  A+  GH  IV  L  +G+ V++ T+  +T L  A   G   IV+ L++  A + + +++G   L +A  + H +    L+  GA+ +  +  G T L    + G  +++ LLL + +R
Sbjct:    2 HSESNQNSGKMNMKGDDNASFLRAARGGNLEKVLEYLKQSTNINSCNAN---------------GLNALHLASKEGHVEIVSELLSRGSIVDAATRKGNTALHIASLAGQDAIVKMLVDHDATVNLQSQNGFXPLYMAAQENHVQAVHLLLASGANQSLATEDGFTPLAVALQQGHDQVVTLLLESDSR 175          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000007044 (supercontig:LSalAtl2s:LSalAtl2s3:1098277:1107147:-1 gene:EMLSAG00000007044 transcript:EMLSAT00000007044 description:"maker-LSalAtl2s3-snap-gene-11.12")

HSP 1 Score: 94.7449 bits (234), Expect = 3.428e-20
Identity = 74/253 (29.25%), Postives = 115/253 (45.45%), Query Frame = 0

Query:   65 NGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELL------GATFVDKKRDMLGSYKLWKQAMEERIVDGKLVY 310
            +G+T L  A   G+KD+V  L+           S     E  +   PL  AA  GH+ +V  L + G+ +N       TP   AC+ G  +    LI+ G +I V  +   + L  A  +GH  I + L+E    V+     G TAL   A  G +EI++LLL +GA +D V  Y  TPLL A+    + +V+ ++ K   V +  + D L  L      G T +  K    G+Y      ++++  D  L+Y
Sbjct:   93 DGSTLLHFAASKGNKDIVSVLLNH---------SADVQAEDFQKCNPLHVAAREGHNDVVHELIDAGSFINQIDMGMWTPFMWACYYGRTDTASVLIDCGCEINVRGQQQVSPLNWAAGRGHENIVRLLLEKNMIVDLGDKYGTTALIWAARKGYMEIVRLLLRHGANVDAVGMYSWTPLLVATAGNFIQVVDLILEKNPNVNKVDK-DGLTALAIACKEGYTEIAFKLISFGAYI----NLQDKYGDTNLMY 331          

HSP 2 Score: 94.3597 bits (233), Expect = 3.901e-20
Identity = 80/252 (31.75%), Postives = 115/252 (45.63%), Query Frame = 0

Query:   35 AARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIER------------------CYANVEQPGSVTFD-GETI-----EGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDV-DAYGMTPLLAASVTGHMHIVEFLISK 261
            AA  G    + V L+H A  + +     K N   PL +A R GH DVV  LI+                   CY       SV  D G  I     +   PL  AA  GH +IVRLL E+   V+   K  +T L  A   G+ EIV+ L+  GA+++    +  T L++A      ++   ++E   +VN+    G TAL    + G  EI   L+S GA +++ D YG T L+ A+  GH  IV+ L+ K
Sbjct:  101 AASKGNKDIVSVLLNHSADVQAEDF--QKCN---PLHVAAREGHNDVVHELIDAGSFINQIDMGMWTPFMWACYYGRTDTASVLIDCGCEINVRGQQQVSPLNWAAGRGHENIVRLLLEKNMIVDLGDKYGTTALIWAARKGYMEIVRLLLRHGANVDAVGMYSWTPLLVATAGNFIQVVDLILEKNPNVNKVDKDGLTALAIACKEGYTEIAFKLISFGAYINLQDKYGDTNLMYAAKGGHKSIVDALLKK 347          

HSP 3 Score: 86.6557 bits (213), Expect = 1.069e-17
Identity = 64/197 (32.49%), Postives = 94/197 (47.72%), Query Frame = 0

Query:   66 GATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAY-GMTPLLAASVTGHMHIVEFLISK 261
            G T L+ A R G+ ++V  L+ R  ANV+  G  ++         PL  A A     +V L+ E+  NVN   K   T L  AC +G+ EI   LI  GA I + +++G T LM A   GH  I   L++  AD+N +     T LH   + G   I+K +L+    +++    G T LL A    +  IV+ L  K
Sbjct:  226 GTTALIWAARKGYMEIVRLLL-RHGANVDAVGMYSW--------TPLLVATAGNFIQVVDLILEKNPNVNKVDKDGLTALAIACKEGYTEIAFKLISFGAYINLQDKYGDTNLMYAAKGGHKSIVDALLKKHADINMRGKDNRTCLHCAIDKGHTSIVKSILNVNPDLELRTIDGDTALLRAVKCRNEDIVQMLXXK 413          

HSP 4 Score: 75.485 bits (184), Expect = 2.956e-14
Identity = 83/301 (27.57%), Postives = 134/301 (44.52%), Query Frame = 0

Query:   35 AARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMD-VDAYGMTPLLAASVTGHMHIVEFLISK---TELVTRKSR-------IDA---LELLGATFVDKK-------RDMLGSYKLWKQAMEERIVDGKLVYPKQV 314
            AAR G +  +++ L H A  +   + S      TPL++A       VV+ ++E+   NV +   V  DG T      L  A   G++ I   L   GA +N   K   T L  A   GH  IV  L+++ ADI +  +   TCL  A  KGH  I K ++ +  D+  +++ G+TAL    +  + +I+++L    A++   D    T L  A       IVE L+     ++L+ R +R       ID      +LG  F  +K        +MLG Y L+  ++ + + +  L  P  V
Sbjct:  233 AARKGYMEIVRLLLRHGANVDAVGMYSW-----TPLLVATAGNFIQVVDLILEKN-PNVNK---VDKDGLT-----ALAIACKEGYTEIAFKLISFGAYINLQDKYGDTNLMYAAKGGHKSIVDALLKKHADINMRGKDNRTCLHCAIDKGHTSIVKSILNVNPDLELRTIDGDTALLRAVKCRNEDIVQMLXXKKAKLXATDTKQDTVLHIAMRARSKAIVEILLRNPKHSQLLYRPNRSGETPYTIDINSNKSILGQVFGARKLNTNEDGENMLG-YDLYSSSLADILTEPSLSMPITV 518          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000012230 (supercontig:LSalAtl2s:LSalAtl2s88:545971:572877:1 gene:EMLSAG00000012230 transcript:EMLSAT00000012230 description:"augustus_masked-LSalAtl2s88-processed-gene-5.3")

HSP 1 Score: 92.0485 bits (227), Expect = 2.101e-19
Identity = 49/156 (31.41%), Postives = 87/156 (55.77%), Query Frame = 0

Query:  107 EGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDV---DAYGMTPLLAASVTGHMHIVEFLI 259
            +G   ++ AA  GH  I++ L  QG ++N   KT  + L  AC  GH  +V+YL +   ++++ + H  + + +AC+ G  KI + L + GA+ N ++  G TALH  A  G LE +K L+   +++++   D YG TPLL +    + ++  +L+
Sbjct:  194 QGDSAVYWAARQGHEDIIQYLVSQGVHINQQNKTGESCLHTACKYGHHAVVEYLSKTHINLDLQDSHLDSAMHVACWHGFPKIVQTLCQAGANANLRNEDGETALHAAASRGHLECLKGLVEX-SKVEIDEKDVYGNTPLLLSIRRHYTNVAMYLL 348          

HSP 2 Score: 72.4034 bits (176), Expect = 2.695e-13
Identity = 58/221 (26.24%), Postives = 92/221 (41.63%), Query Frame = 0

Query:   66 GATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFE----------------------------------IVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDV-DAYGMTPLLAASVTGH 251
            G + L  AC+ GH  VVEYL  + + N++           ++ A  +  A   G   IV+ L + GAN N   +   T L AA   GH E                                  +  YL+  G D ++ N++G + + I   +G   +A+ L   G  V+  +  G+  +H  A+ G  EI++ L   G R+DV +   +TP +AA   GH
Sbjct:  228 GESCLHTACKYGHHAVVEYL-SKTHINLD------LQDSHLDSA--MHVACWHGFPKIVQTLCQAGANANLRNEDGETALHAAASRGHLECLKGLVEXSKVEIDEKDVYGNTPLLLSIRRHYTNVAMYLLHNGVDFDIVNKNGDSPIHITSSEGLLGLAQSLCAFGCSVDTSNKNGDYPIHYAAKEGQTEILRCLCLAGXRIDVXNKENVTPEMAALAQGH 439          

BLAST of FEM-1 vs. L. salmonis genes
Match: EMLSAG00000005649 (supercontig:LSalAtl2s:LSalAtl2s3041:29583:35919:1 gene:EMLSAG00000005649 transcript:EMLSAT00000005649 description:"maker-LSalAtl2s3041-augustus-gene-0.6")

HSP 1 Score: 83.5741 bits (205), Expect = 1.273e-17
Identity = 76/231 (32.90%), Postives = 111/231 (48.05%), Query Frame = 0

Query:   23 THKMEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIER-CYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEV-ANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYG-MTPLLAASVTG 250
            T  ++ R  +  AA  G L  L+  L   +  E+      K + ATPL  A  + + + +  LI   C  N          G    G+  L CA  A     VRLL E GA  N+    + TPL  A   G+ E+V+ LIE GA +EV       T L +A  +G+ +  +YLIE GA V+ ++ +G +ALH  A S S E +++LL +GA  +   +   TPL +A V G
Sbjct:   97 TKDLDGRTCLHLAACSGDLETLRTLL---SSPEINVNSFDKQHQATPLFCAALSSNTEAIRSLIASGCDINA---------GLHDHGSTALHCAVKANAVENVRLLLESGAVPNNVLLFSETPLHTAASMGYGEVVKLLIENGACLEVLMGTMKMTALHLAAQEGNSECVRYLIEGGASVDIRNGRGQSALHLAALSQSPETVEILLKSGANPNCGDFDRRTPLHSAIVKG 315          

HSP 2 Score: 60.8474 bits (146), Expect = 3.695e-10
Identity = 53/172 (30.81%), Postives = 81/172 (47.09%), Query Frame = 0

Query:   66 GATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKT-NSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESG--SLEIMKLLLSNGARMD 234
            G+T L  A +    + V  L+E        P +V    ET     PL  AA+ G+  +V+LL E GA +     T   T L  A  +G+ E V+YLIE GA +++ N  G + L +A      +  + L++ GA+ N       T LH     G  S + ++LLL  GA ++
Sbjct:  171 GSTALHCAVKANAVENVRLLLESGAV----PNNVLLFSET-----PLHTAASMGYGEVVKLLIENGACLEVLMGTMKMTALHLAAQEGNSECVRYLIEGGASVDIRNGRGQSALHLAALSQSPETVEILLKSGANPNCGDFDRRTPLHSAIVKGSRSYDCVRLLLDTGADVN 333          

BLAST of FEM-1 vs. SwissProt
Match: gi|123916200|sp|Q2T9K6.1|FEM1C_XENLA (RecName: Full=Protein fem-1 homolog C; Short=FEM1c; AltName: Full=FEM1-gamma)

HSP 1 Score: 719.539 bits (1856), Expect = 0.000e+0
Identity = 361/632 (57.12%), Postives = 460/632 (72.78%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLL-KGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+ +  VFNAARDGKLR L   L+++AKD+V  L+S KTNGATPL++A R GH D+V+YL+++C A+VE  GSV FDGETIEGAPPLW A+AAGH  +VR L   GA+VN+TT TNSTPLRAACFDGH EIV+YL+E  AD+EVANRHGHTCLMI+CYKGH +IA++L+E GADVNRKSVKGNTALHDCAESGSLEIM++LL  GARM+ D YGMTPLL+ASVTGH +IV+FL    +  ++  RI+ALELLGATFVDKKRD+LG+ K WK+AM+ R  D   +  K    + I AY+   EV + ++L ++I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+F+RCI LW YALDMQQ  L+PLSPMT SSLLSFAELFSFM+   ++R+     + V F D+M +  K+V E+          P        D    N+ L IILHLICLL K+    SPDQ H  K ++Y FLKLHP+G+N  +PLHLA  ++++ VGRYP+C+FPS     +L+ECGAD N +D + N+ LHVAA N    P ++  L+ +G H D+ NS  +T   LL + +    +I P+ +T LQCLAA+VI +  I Y+  +  +LE FV +H
Sbjct:    1 MDLKTAVFNAARDGKLRLLSKLLENKAKDDVVLLMSEKTNGATPLLMAARYGHLDMVDYLLDQCSASVEIGGSVNFDGETIEGAPPLWAASAAGHLKVVRSLLVHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKADLEVANRHGHTCLMISCYKGHKEIAQFLLEKGADVNRKSVKGNTALHDCAESGSLEIMQMLLKYGARMEKDGYGMTPLLSASVTGHTNIVDFLTQNPQ-TSKNERINALELLGATFVDKKRDLLGALKYWKRAMDMRHSDRTNIVSKPEPQTLIMAYDYAREVNTAEELDNLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFKRCINLWKYALDMQQNNLDPLSPMTASSLLSFAELFSFML---QDRAKGLLGTTVTFDDLMGILCKSVMEIDRAVKQTAPPP--------DQVQLNKALSIILHLICLLEKV--PCSPDQDHFKKQNIYRFLKLHPKGKNNFSPLHLAVDKNTTCVGRYPVCKFPSFQVTAILLECGADVNVRDAEQNSPLHVAALNN--HPDIMNLLVKSGAHFDSTNSHNQTACDLLDEKEMAKNLIQPINHTTLQCLAARVIVKHNIQYKQEIPEKLESFVLLH 616          

BLAST of FEM-1 vs. SwissProt
Match: gi|74751963|sp|Q96JP0.1|FEM1C_HUMAN (RecName: Full=Protein fem-1 homolog C; Short=FEM1c; AltName: Full=FEM1-gamma)

HSP 1 Score: 719.153 bits (1855), Expect = 0.000e+0
Identity = 363/634 (57.26%), Postives = 463/634 (73.03%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKT--PANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHE-VINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+ +  VFNAARDGKLR L   L  ++K+EV  L+S KTNGATPL++A R GH D+VE+L+E+C A++E  GSV FDGETIEGAPPLW A+AAGH  +V+ L   GA+VN+TT TNSTPLRAACFDGH EIV+YL+E  AD+EV+NRHGHTCLMI+CYKGH +IA+YL+E GADVNRKSVKGNTALHDCAESGSL+IMK+LL   A+M+ D YGMTPLL+ASVTGH +IV+FL    +  ++  RI+ALELLGATFVDKKRD+LG+ K WK+AM  R  D   +  K V  + I AY+   EV S ++L  +I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+F+RCI LW YALDMQQ  L+PLSPMT SSLLSFAELFSFM+   ++R+     + V F D+M +  K+V E+      IK+T  PA+ +         N+ L IILHLICLL K+   L  D  H  K ++Y FLKLHPRG+N  +PLHLA  ++++ VGRYP+C+FPSL    +L+ECGAD N +D+D N+ LH+AA N    P ++  L+ +G H DA N   +T   LL  + + + +I P+ +T LQCLAA+VI    I Y+G +  +LE FV++H
Sbjct:    1 MDLKTAVFNAARDGKLRLLTKLLASKSKEEVSSLISEKTNGATPLLMAARYGHLDMVEFLLEQCSASIEVGGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLNHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKADLEVSNRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLDIMKMLLMYCAKMEKDGYGMTPLLSASVTGHTNIVDFLTHHAQ-TSKTERINALELLGATFVDKKRDLLGALKYWKKAMNMRYSDRTNIISKPVPQTLIMAYDYAKEVNSAEELEGLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFML---QDRAKGLLGTTVTFDDLMGILCKSVLEIERA---IKQTQCPADPLQ-------LNKALSIILHLICLLEKVPCTLEQD--HFKKQTIYRFLKLHPRGKNNFSPLHLAVDKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNN--HPDIMNLLIKSGAHFDATNLHKQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGHIPEKLETFVSLH 616          

BLAST of FEM-1 vs. SwissProt
Match: gi|171769796|sp|A7MB89.1|FEM1C_BOVIN (RecName: Full=Protein fem-1 homolog C; Short=FEM1c; AltName: Full=FEM1-gamma)

HSP 1 Score: 719.153 bits (1855), Expect = 0.000e+0
Identity = 363/634 (57.26%), Postives = 463/634 (73.03%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKT--PANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHE-VINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+ +  VFNAARDGKLR L   L  ++K+EV  L+S KTNGATPL++A R GH D+VE+L+E+C A++E  GSV FDGETIEGAPPLW A+AAGH  +V+ L   GA+VN+TT TNSTPLRAACFDGH EIV+YL+E  AD+EV+NRHGHTCLMI+CYKGH +IA+YL+E GADVNRKSVKGNTALHDCAESGSL+IMK+LL   A+M+ D YGMTPLL+ASVTGH +IV+FL    +  ++  RI+ALELLGATFVDKKRD+LG+ K WK+AM  R  D   +  K V  + I AY+   EV S ++L  +I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+F+RCI LW YALDMQQ  L+PLSPMT SSLLSFAELFSFM+   ++R+     + V F D+M +  K+V E+      IK+T  PA+ +         N+ L IILHLICLL K+   L  D  H  K ++Y FLKLHPRG+N  +PLHLA  ++++ VGRYP+C+FPSL    +L+ECGAD N +D+D N+ LH+AA N    P ++  L+ +G H DA N   +T   LL  + + + +I P+ +T LQCLAA+VI    I Y+G +  +LE FV++H
Sbjct:    1 MDLKTAVFNAARDGKLRLLTKLLASKSKEEVSSLISEKTNGATPLLMAARYGHLDMVEFLLEQCSASIEVGGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLNHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKADLEVSNRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLDIMKMLLMYCAKMEKDGYGMTPLLSASVTGHTNIVDFLTHHAQ-TSKTERINALELLGATFVDKKRDLLGALKYWKKAMNMRYSDRTNIISKPVPQTLIMAYDYAKEVNSAEELEGLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFKRCINLWKYALDMQQNNLDPLSPMTASSLLSFAELFSFML---QDRAKGLLGTTVTFDDLMGILCKSVLEIERA---IKQTQCPADPLQ-------LNKALSIILHLICLLEKVPCTLEQD--HFKKQTIYRFLKLHPRGKNNFSPLHLAVDKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNN--HPDIMNLLIKSGAHFDATNLHKQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGHIPEKLETFVSLH 616          

BLAST of FEM-1 vs. SwissProt
Match: gi|172045553|sp|Q9VFD5.2|FEM1A_DROME (RecName: Full=Protein fem-1 homolog CG6966)

HSP 1 Score: 717.613 bits (1851), Expect = 0.000e+0
Identity = 368/670 (54.93%), Postives = 465/670 (69.40%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDR--SPVNFCDIMTVFNKAVEEVRMG-----------QLNIKKTP------------------------ANSVSNDRDITYFNRTLIIILHLICLLTKLM--PHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+++ +VFNAARD  L +LK  L +++  EV  L+SAK NGATPLV++CRNGH D+VEYL+ +C ANVEQ GSV+FDGE IE APPLWCAAAAGH  IV++L  +GANVNSTT+TNSTPLRAACFDGH+EIV+YL+  GAD EVANRHGHTCLMIACYKGH++IA+YL+ + ADVNR SVKGNTALHDCAESGSL+I++LLL +GA MDVD YGMTPLLAASVTGHM IVE LI+    V+R+SRI ALELLGAT+VD+KRDM  +  LW++A+EER V+  L   ++ +  P+ AYE   EV S+++L +++ DPD MRMQAL++R+RILGP HPDTSYYIR+RGA YAD G F+RCI LW YAL MQQK+L+PLSPMTQSSLLSFAELFSFM+ E   R  P  R   P+    ++T+F KAV+EV  G           Q   K+ P                        A+    + D    +RT+I  +H+ CLL+ L+    LSP+   +V  ++Y   +L  R R   T LH AC R+ +  GRYP CQFPS+   + L+E GADPNA D   NT LH+A      +P L   LL+ G HLD  N  G+TFESLL    +H++I+PMKYT L CLAA+ I++  I Y G +   L  F+ +H
Sbjct:    1 MDYKFIVFNAARDNNLAQLKATLYNKSSVEVGSLISAKVNGATPLVISCRNGHYDIVEYLLTKCRANVEQVGSVSFDGEPIEDAPPLWCAAAAGHLGIVKMLVRRGANVNSTTRTNSTPLRAACFDGHYEIVKYLVHHGADFEVANRHGHTCLMIACYKGHFRIAQYLLSLNADVNRCSVKGNTALHDCAESGSLQILQLLLKHGATMDVDYYGMTPLLAASVTGHMPIVEHLIT-LPCVSRESRIHALELLGATYVDRKRDMAAALNLWRRALEERAVEPPL---EKKVQEPVPAYEMVREVTSVEELEEMVLDPDEMRMQALVIRQRILGPTHPDTSYYIRFRGAHYADAGRFDRCIELWSYALTMQQKILQPLSPMTQSSLLSFAELFSFMLVEA-GRLLPRGRVVPPIEPDSMLTIFYKAVKEVERGLAFTLEQQKDQQHPQKQLPAADKSPSCSASSSASSSSSTTLLSAHQHDCNHDPNALSRTMISAIHIGCLLSSLLDTDALSPEMRRQVMGALYRLNRLKVRVRFDRTALHYACYREGTLAGRYPSCQFPSVTLAKALLEVGADPNAIDEAGNTPLHLATMQPYVEP-LSHILLEGGAHLDTKNYAGETFESLLAPTPMHKIIDPMKYTTLACLAARTIKKHDIRYEGTVPATLYEFIELH 664          

BLAST of FEM-1 vs. SwissProt
Match: gi|81900078|sp|Q8CEF1.1|FEM1C_MOUSE (RecName: Full=Protein fem-1 homolog C; Short=FEM1c; AltName: Full=FEM1-gamma)

HSP 1 Score: 716.842 bits (1849), Expect = 0.000e+0
Identity = 362/634 (57.10%), Postives = 463/634 (73.03%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKT--PANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHE-VINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+ +  VFNAARDGKLR L   L  ++K EV  L+S KTNGATPL++A R GH D+VE+L+E+C A++E  GSV FDGETIEGAPPLW A+AAGH  +V+ L   GA+VN+TT TNSTPLRAACFDGH EIV+YL+E  AD+EV+NRHGHTCLMI+CYKGH +IA+YL+E GADVNRKSVKGNTALHDCAESGSL+IMK+LL   A+M+ D YGMTPLL+ASVTGH +IV+FL    +  ++  RI+ALELLGATFVDKKRD+LG+ K WK+AM  R  D   +  K V  + I AY+   EV S ++L  +I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+F+RCI LW YALDMQQ  L+PLSPMT SSLLSFAELFSFM+   ++R+     + V F D+M +  K+V E+      IK+T  PA+ +         N+ L IILHLICLL K+    + +Q H  K ++Y FLKLHPRG+N  +PLHLA  ++++ VGRYP+C+FPSL    +L+ECGAD N +D+D N+ LH+AA N    P ++  L+ +G H DA N   +T   LL  + + + +I P+ +T LQCLAA+VI    I Y+G +  +LE FV++H
Sbjct:    1 MDLKTAVFNAARDGKLRLLTKLLASKSKAEVSSLISEKTNGATPLLMAARYGHLDMVEFLLEQCSASIEVGGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLNHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKADLEVSNRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLDIMKMLLMYCAKMEKDGYGMTPLLSASVTGHTNIVDFLTHHAQ-TSKTERINALELLGATFVDKKRDLLGALKYWKKAMNMRYSDRTNIISKPVPQTLIMAYDYAKEVNSAEELEGLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFML---QDRAKGLLGTTVTFDDLMGILCKSVLEIERA---IKQTQCPADPLQ-------LNKALSIILHLICLLEKV--PCTVEQDHFKKQTIYRFLKLHPRGKNNFSPLHLAVDKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNN--HPDIMNLLIKSGAHFDATNLHKQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGNIPEKLETFVSLH 616          

BLAST of FEM-1 vs. SwissProt
Match: gi|82202503|sp|Q6P9Z4.1|FEM1A_DANRE (RecName: Full=Protein fem-1 homolog A; Short=FEM1a; AltName: Full=FEM1-alpha)

HSP 1 Score: 687.952 bits (1774), Expect = 0.000e+0
Identity = 353/633 (55.77%), Postives = 453/633 (71.56%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASP-ISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSV-HEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+    VFNAARDGKL+ ++  L +++ +E   L   +T G TPL++A R GH  VV +L+ERC ANV   GSV FDGETIEGAPPLW A+AAGH  +V+ L E GA VN+TT TNSTPLRAACFDGH EIV+YL+E  AD+EVANRHGHTCLMI+CYKGH +IA++L+E GADVNRKSVKGNTALHDCAESGSLEIMK+LL   ARM+ D YGMTPLLAASVTGH +IVEFL+ +    +R+ RI ALELLGATFVDKKRD+LG+ + W++AME R   G+    ++  A P + AY+ + EV++ ++L  +I+DPD MRMQALLVRERILGPAHPDTSYYIRYRGAVYAD G+FERCI LW YALDMQQ  LEPLSPMT SS LSFAELFSF++   ++R+     + V+F D+M V +K+V EV       ++ P        +   F++ L IILHL+           P+Q H  + +VY  LKL+PR R G+TPLH+A  RD++SVGRYP+ +FPSL    LL+ECGAD +++D D+NT LH+AA N    P ++  L+ AG H DA N+  +T   LL+ QS     ++P+ +T LQCLAA+ +    + Y+G +  Q+E F+ +H
Sbjct:    1 MDISAAVFNAARDGKLKLMQKLLINKSPEERAALAEERTEGGTPLLIAARYGHLPVVHFLLERCGANVALGGSVNFDGETIEGAPPLWAASAAGHLPVVKALLEHGAPVNNTTLTNSTPLRAACFDGHLEIVRYLVEHQADLEVANRHGHTCLMISCYKGHREIAQFLLEKGADVNRKSVKGNTALHDCAESGSLEIMKMLLKCDARMERDGYGMTPLLAASVTGHTNIVEFLVHQPR-ASREQRIHALELLGATFVDKKRDLLGAMRYWRRAMELRWAGGQAGALEKPTAGPLVPAYDCSREVSTAEELEALITDPDDMRMQALLVRERILGPAHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQSNLEPLSPMTASSFLSFAELFSFVL---QDRAKGTLAARVSFQDLMGVLSKSVREVERAVAQRERPP--------EPPQFSKALSIILHLL--FLLQKLRCGPEQEHLKRQTVYRLLKLNPRARGGHTPLHMAVDRDTTSVGRYPVGRFPSLAVASLLLECGADVDSRDYDNNTPLHIAAAN--GCPDIMAALIRAGAHFDATNAAQQTAYQLLEAQSSGRHALHPLNHTTLQCLAARAVTAHRLPYKGLISEQMEAFIELH 617          

BLAST of FEM-1 vs. SwissProt
Match: gi|172045713|sp|Q7T3P8.2|FEM1C_DANRE (RecName: Full=Protein fem-1 homolog C; Short=FEM1c; AltName: Full=FEM1-gamma)

HSP 1 Score: 681.019 bits (1756), Expect = 0.000e+0
Identity = 354/632 (56.01%), Postives = 457/632 (72.31%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLK-GQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+ +  VFNAARDGKL+ L+  L++++  EV +L++ KTNGATPL++A R GH ++VE+L+E C A VE  GSV FDGE IEGAPPLW A+AAGH  +V+ L   GA+VN+TT TNSTPLRAACFDGH +IV+YL+E  AD+EVANRHGHTCLMI+CYKGH +IA++L+E GADVNR+SVKGNTALHDCAESGSLEIMK+LL  GA M+ D YGMTPLL+ASVTGH +IV+FL +  +      RI ALELLGATFVDKKRD+LG+ + WK+AME R  +   +  ++ +  P  AY+ + EV++ ++L  +I+DPD MRMQALL+RERILGPAHPDTSYYIRYRGAVYAD G+FERCI LW YALDMQQ  LEPLSPMT SSLLSFAELFSFM+   ++R+     + V+F  +M +  ++V E+       + +P      D D    ++ L IILHLICLL KL    SP+Q    K ++Y FLKL   GRNG +PLHLA   +++ VGRYP+C+FPSL    +L+ECGAD N++D D N+ LHVAA N    P +++ L+  G H D+ N+  +T   LL   Q    +I P+ +T LQCLAA+ I R  + YRG++  +LE FV +H
Sbjct:    1 MDLKTAVFNAARDGKLKLLQKLLENKSDREVLKLMAEKTNGATPLLMASRYGHLELVEFLMECCCAPVELGGSVNFDGEVIEGAPPLWAASAAGHLKVVQSLLGHGASVNNTTLTNSTPLRAACFDGHLDIVRYLVEHQADLEVANRHGHTCLMISCYKGHREIAQFLLEKGADVNRRSVKGNTALHDCAESGSLEIMKMLLKFGASMEKDGYGMTPLLSASVTGHTNIVDFLTAHPQ-TGLAERISALELLGATFVDKKRDLLGALQYWKRAMELRHSEADCMRREEPL-EPEPAYDCSREVSTAEELDGLIADPDDMRMQALLIRERILGPAHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQSNLEPLSPMTASSLLSFAELFSFML---QDRAKGLLGAGVSFQQLMEILCRSVLEIERAVKQPRPSP------DPDPAQLSKLLSIILHLICLLEKL--PCSPEQDQHKKETIYRFLKLQACGRNGFSPLHLAVDHNTTCVGRYPVCKFPSLQVASVLLECGADVNSRDLDDNSPLHVAASNN--HPDIMKLLISGGTHFDSTNALQQTACDLLDHTQLAKSLIQPINHTTLQCLAARAIIRHRLVYRGSIPERLEAFVLLH 617          

BLAST of FEM-1 vs. SwissProt
Match: gi|122145914|sp|Q29RM5.1|FEM1A_BOVIN (RecName: Full=Protein fem-1 homolog A; Short=FEM1a; AltName: Full=FEM1-alpha)

HSP 1 Score: 674.47 bits (1739), Expect = 0.000e+0
Identity = 350/668 (52.40%), Postives = 458/668 (68.56%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLI-ERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTEL-----------------------------------VTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKT-FESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+    V+NAARDGKL+ L+  L  R+++E+++L     +G TPL++A R GH DVVEYL++RC A+VE  GSV FDGE IEGAPPLW A+AAGH  +VR L  +GA+VN TT+TNSTPLRAACFDGH E+V+YL+ E  AD+EVANRHGHTCLMI+CYKGH +IA+YL+E GA VNR+S KGNTALHDCAESGSLEI++LLL   ARM+ D YGMTPLLAASVTGH +IVE+LI +                                       +R++ ++ALELLGAT+VDKKRD+LG+ K W++AME R   G+ + PK      + AY+ + EV + ++L  +I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+FERCI LW YALDMQQ  LEPLSPMT SS LSFAELFS+++ +   + +    +P+ F D+M V  K V EV    L + K P +S         F + L IILHL+ LL K+    +PDQ H    +VY  LK  PRG+NG TPLH+A   ++++VGRYP+ +FPSL  V++L++CGADP+++D D+NT LH+AAQN    P ++  L++AG H+DA N+  KT +E L +       I P  Y  LQCLAA+ + +  I Y+G +  +LE F+ +H
Sbjct:    1 MDLHTAVYNAARDGKLQLLQKLLSGRSREELEELTGEVASGGTPLLIAARYGHLDVVEYLVDRCGASVEAGGSVHFDGEIIEGAPPLWAASAAGHLDVVRSLLRRGASVNRTTRTNSTPLRAACFDGHLEVVRYLVGEHQADLEVANRHGHTCLMISCYKGHREIARYLLEQGAQVNRRSAKGNTALHDCAESGSLEILQLLLGCNARMERDGYGMTPLLAASVTGHTNIVEYLIQEQPAGDEQAQPGLARVQPQGARSSPEEPPSGESYESCCPTSREAAVEALELLGATYVDKKRDLLGALKHWRRAMELRHQGGEYL-PKPEPPQLVLAYDYSREVNTTEELEALITDPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQNNLEPLSPMTASSFLSFAELFSYVLQDRSAKGSLG--TPIGFADLMGVLCKGVREVERA-LQLPKEPGDSAQ-------FTKALAIILHLLYLLEKV--ECTPDQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDAETTNVGRYPVGRFPSLQVVKVLLDCGADPDSRDFDNNTPLHIAAQNN--CPGIMNALIEAGAHMDATNAFKKTAYELLDEKLLAKSTIQPFNYVTLQCLAARALDKNKIPYKGFIPEELEAFIELH 653          

BLAST of FEM-1 vs. SwissProt
Match: gi|81907903|sp|Q4V890.1|FEM1A_RAT (RecName: Full=Protein fem-1 homolog A; Short=FEM1a; AltName: Full=FEM1-alpha)

HSP 1 Score: 665.226 bits (1715), Expect = 0.000e+0
Identity = 349/669 (52.17%), Postives = 456/669 (68.16%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLI-ERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLI---------SKTEL---------------------------VTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSV-HEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+    V+NAA DGKL  L+  L  R ++E+++L+     G TPL++A R GH DVVEYL++ C A+VE  GSV FDGETIEGAPPLW A+AAGH ++VR L  +GA+VN TT+TNSTPLRAACFDGH ++V+YL+ E  AD+EVANRHGHTCLMI+CYKGH +IA+YL+E GA VNR+S KGNTALHDCAESGSLEI++LLL   ARM+ D YGMTPLLAASVTGH +IVE+LI         S TEL                            +R++ ++ALELLGAT+VDKKRD+LG+ K W++AME R   G  + PK      + AY+ + EV++  +L  +I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+FERCI LW YALDMQQ  LEPLSPMT SS LSFAELFS+++ +   + N   +  + F D+M V +K V EV    L + K P +S         F + + IILHL+ LL K+    +P Q H    +VY  LK  PRG+NG TPLH+A  +++++VGRY +  FPSL  V++L++CGADP+++D D+NT LHVAAQN    P ++  L++AG H+DA N+  KT   LL  + +    + P  Y  LQCLAA+ + R  + Y+G +  +LE F+ +H
Sbjct:    1 MDLHTAVYNAAHDGKLLLLQKLLAGRGREEIEELLGEVAGGGTPLLIAARRGHLDVVEYLVDNCGASVEASGSVHFDGETIEGAPPLWAASAAGHLAVVRSLLRRGASVNRTTRTNSTPLRAACFDGHLDVVRYLVGEHKADLEVANRHGHTCLMISCYKGHREIARYLLERGAQVNRRSAKGNTALHDCAESGSLEILQLLLGCHARMERDGYGMTPLLAASVTGHTNIVEYLIQEQPGHGQLSGTELPGEGSSQMAGNHCSTPEEAEPYESCCPTSREAAVEALELLGATYVDKKRDLLGALKHWRRAMELRHQGGDYL-PKPEPQQLVLAYDYSREVSTPQELEALITDPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQNNLEPLSPMTASSFLSFAELFSYVLQDRSAKGNLGMQ--LGFADLMGVLSKGVREVERA-LQLPKEPGDSAQ-------FTKAIAIILHLLYLLEKV--ECTPSQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDKETTNVGRYRVGIFPSLQVVKVLLDCGADPDSRDFDNNTPLHVAAQNN--CPAIMDALIEAGAHMDATNAFKKTAYELLDSKLLAKSTMQPFNYVTLQCLAARALDRNKVPYKGFIPEELEAFIQLH 654          

BLAST of FEM-1 vs. SwissProt
Match: gi|81907788|sp|Q9Z2G1.1|FM1AA_MOUSE (RecName: Full=Protein fem-1 homolog A-A; Short=FEM1a-A; AltName: Full=FEM1-alpha-A)

HSP 1 Score: 659.833 bits (1701), Expect = 0.000e+0
Identity = 348/669 (52.02%), Postives = 456/669 (68.16%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLI-ERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLI---------SKTEL---------------------------VTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHE-VINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+    V+NAA DGKL  L+  L  R ++E+++L+     G TPL++A R GH DVVEYL++ C A+VE  GSV FDGETIEGAPPLW A+AAGH ++VR L  +GA+VN TT+TNSTPLRAACFDGH ++V+YL+ E  AD+EVANRHGHTCLMI+CYKGH +IA+YL+E GA VNR+S KGNTALHDCAESGSLEI++LLL   ARM+ D YGMTPLLAASVTGH +IVE+LI         S TEL                            +R++ ++ALELLGAT+VDKKRD+LG+ K W++AME R   G  + PK      + AY+ + EV +  +L  +I+DPD MRMQALL+RERILGP+HPDTSYYIRYRGAVYAD G+FERCI LW YALDMQQ  LEPLSPMT SS LSFAELFS+++ +   + N   +  + F D+M V +K V EV    L + K P +S         F + + IILHL+ LL K+    +P Q H    +VY  LK  PRG+NG TPLH+A  +++++VGRY +  FPSL  V++L++CGADP+++D D+NT LH+AAQN    P ++  L++AG H+DA N+  KT   LL  + + +  + P  Y  LQCLAA+ + R  + Y+G +  +LE F+ +H
Sbjct:    1 MDLHTAVYNAAHDGKLPLLQKLLAGRGREELEELLGEVAGGGTPLLIAARRGHLDVVEYLVDHCGASVEASGSVHFDGETIEGAPPLWAASAAGHLAVVRSLLRRGASVNRTTRTNSTPLRAACFDGHLDVVRYLVGEHKADLEVANRHGHTCLMISCYKGHREIARYLLERGAQVNRRSAKGNTALHDCAESGSLEILQLLLGCHARMERDGYGMTPLLAASVTGHTNIVEYLIQEQPGHEQLSGTELPGEGSSQVAGNHCSTPEEAEPYESCCPTSREAAVEALELLGATYVDKKRDLLGALKHWRRAMELRHQGGGYL-PKPEPQQLVLAYDYSREVTTPQELEALITDPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQNNLEPLSPMTASSFLSFAELFSYVLQDRSAKGNLGMQ--LGFADLMGVLSKGVREVERA-LQLPKEPGDSAQ-------FTKAIAIILHLLYLLEKV--ECTPSQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDKETTNVGRYRVGVFPSLQVVKVLLDCGADPDSRDFDNNTPLHIAAQNN--CPAIMDALIEAGAHMDATNAFKKTAYELLDSKLLAKSTVQPFNYVTLQCLAARALDRNKVPYKGFIPEELEAFIQLH 654          

BLAST of FEM-1 vs. nr
Match: gi|1325270978|ref|XP_023320099.1| (protein fem-1 homolog C-like [Eurytemora affinis])

HSP 1 Score: 954.896 bits (2467), Expect = 0.000e+0
Identity = 460/655 (70.23%), Postives = 536/655 (81.83%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENR---------------------NRSNPNDRS---PVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            MEF+NVVFNAARDGKLRRLKVFLDHR K+EV +LV+A+TNGATPLVL+CRNGH +VV+YL++RC ANVEQ GSVTFDGETIEGAPPLWCAAAAGH  IV+LL + GANVNSTTKTNSTPLRAACFDGH+EIV+YL+E  ADIEVANRHGHTCLMIACYKGH+KIA+YLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLL+ +GAR+DVDAYGMTPLLAASVTGH+HIVE L  + ++V ++ RIDALELLGATFVDKKRDMLG+  LWK+AMEER +DG LV PK +++SPISAYENT+EV S+  L +IISDPD+MRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQK+LEPLSPMTQSSLLSFAELFSFMMSE                        R     RS   PV+FCDIMTVF +AV EV+ GQ+ ++           D TYFNRT++I+LHL+CL TKL+PHL   Q+ EVK +VY+F+ +  RGR G T LHLAC ++SSSVGRYPIC FPS+ A++LL+ECGADPNA DND NT LH AA +KP K ++VQTLLDAG H D VNSEGK F +++KGQ +HE+ N +++T L+CL+A+ +RR  ++  G L   LE FV  H
Sbjct:    1 MEFKNVVFNAARDGKLRRLKVFLDHRPKEEVNKLVTARTNGATPLVLSCRNGHHEVVQYLVDRCEANVEQAGSVTFDGETIEGAPPLWCAAAAGHLEIVKLLVKSGANVNSTTKTNSTPLRAACFDGHYEIVKYLVEHNADIEVANRHGHTCLMIACYKGHFKIARYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLIQSGARIDVDAYGMTPLLAASVTGHLHIVEHLTGQRDMVGKQERIDALELLGATFVDKKRDMLGALTLWKRAMEERYLDGVLVIPKALVSSPISAYENTLEVESVSALEEIISDPDSMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKILEPLSPMTQSSLLSFAELFSFMMSEGGRSRGQVPFANLAGGGREGAAIEREEGGGRSSPPPVSFCDIMTVFQRAVREVKAGQVALE----GGEGKGGDSTYFNRTIVILLHLVCLFTKLVPHLPDKQVFEVKQAVYNFVNVGARGRGGATALHLACTKESSSVGRYPICVFPSVPAIQLLLECGADPNAIDNDGNTPLHTAAASKPVKQVVVQTLLDAGAHFDLVNSEGKPFFNIMKGQPLHEIANCVRHTSLRCLSARSVRRHELSTAG-LPRTLELFVEQH 650          

BLAST of FEM-1 vs. nr
Match: gi|902942568|gb|AKS25866.1| (Fem-1C [Eriocheir sinensis])

HSP 1 Score: 887.1 bits (2291), Expect = 0.000e+0
Identity = 425/645 (65.89%), Postives = 515/645 (79.84%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVF----LDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSP-VNFCDIMTVFNKAVEEVRMGQLNIKKTPAN------SVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSS-SVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLL--KGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            MEF+N VFNAARDG LRRLK+     LDHR++ E  ++V A+TNGATPLV+A +NGH   VEYL+ERC A++EQ GSV FDGETI+GAPPLWCAAAAGH  +V  L E+GANVN+TTKTNSTPLRAACFDGHFEIV+YL+E  +DIEVANRHGHTCLMIACYKGH+ IAKYLIEI A+VNRKSVKGNTALHDCAESGSL+IMKLLL + A+MDVD+YGMTPLLAASVTGH HIVE+LIS+T+L++RK +IDALELLGAT+VDKKRDMLG+ + WK AM ER  +G+++YPK V  SPI+AYEN++EV +L+QL +IISDPD MRMQALLVRERILGPAHPDTSYYIRYRGAVYAD G+F+RCITLWMYALDMQQK+LEPLSPMTQSS +SFAELFSFMMSE +NR     R P VNFCD+M VF K + EV +G   + K   +          +RD TYF+RTL+I +HL+CLLTKL P L+P Q H ++ +VY  ++L PRG  G TPLHLAC+RDSS ++GR+PIC FPS + V LL+E GADP A+D+  NT LH  A NK  +  L+  LL AG HLD +N  G++F S+   +GQ +H++INP+++T LQCLAA  +RR G+ Y   L P+L +FV  H
Sbjct:    1 MEFKNGVFNAARDGNLRRLKLLFQMLLDHRSRSEASRMVCARTNGATPLVMAAKNGHLACVEYLVERCSADLEQVGSVVFDGETIDGAPPLWCAAAAGHLVVVSYLLEKGANVNATTKTNSTPLRAACFDGHFEIVKYLVEHNSDIEVANRHGHTCLMIACYKGHFDIAKYLIEITANVNRKSVKGNTALHDCAESGSLDIMKLLLDHNAKMDVDSYGMTPLLAASVTGHTHIVEYLISRTDLISRKDKIDALELLGATYVDKKRDMLGALRFWKMAMSERYDNGEMIYPKPVQQSPIAAYENSIEVMTLNQLEEIISDPDEMRMQALLVRERILGPAHPDTSYYIRYRGAVYADTGNFDRCITLWMYALDMQQKILEPLSPMTQSSFVSFAELFSFMMSEAKNRGR---RVPVVNFCDMMRVFEKCLREVEVGMFQLNKGVTSYGGMLAGGGGERDTTYFHRTLVITMHLVCLLTKLGPGLTPLQRHAMRKAVYDLVRLDPRGSGGVTPLHLACSRDSSATIGRFPICTFPSAEVVNLLLEVGADPCARDHQGNTPLHTLASNKQCRRDLLMALLSAGAHLDTLNRSGQSFASIRSSRGQRLHQLINPLQHTSLQCLAAATLRRHGLPYDNTLHPRLSQFVDRH 642          

BLAST of FEM-1 vs. nr
Match: gi|558761529|gb|AHA90856.1| (FEM-1 [Litopenaeus vannamei])

HSP 1 Score: 882.478 bits (2279), Expect = 0.000e+0
Identity = 425/641 (66.30%), Postives = 508/641 (79.25%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSP-VNFCDIMTVFNKAVEEVRMGQLNIKKTPANSV------SNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSS-SVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLL--KGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            MEF+N VFNAARDG LRRLK+ LDHR++ EV ++VSA+TNGATPLV+A +NGH   VEYL+ERC A++EQ GSV FDGETI+GAPPLWCAAAAGH  +V  L  + ANVN+TTKTNSTPLRAACFDGHF+IV+YL+E  ADIEVANRHGHTCLMIA YKGH+ IAKYLI+I A+VNRKSVKGNTALHDCAESGSLEIMKLLL + A+MDVD+YGMTPLLAASVTGH HIVE+LIS+++LV+RK +IDALELLGAT+VDKKRDMLG+ + WK AM ER     ++YPK V  SPI+AYEN+VEV ++ QL +IISDPD MRMQALLVRERILGPAHPDTSYYIRYRGAVYAD G+F+RCITLWMYALDMQQK+LEPLSPMTQSS +SF ELFSFMMSE +NR     R P VNFCD+M VF K + EV +G   + K   +          +RD TYF+RTL+I +HL+CLLTKL P L+  Q H ++ +VYH +++ PRG  G TPLHLAC+RDSS +VGR+PIC FPSL+ V LL+E GADP A D   NT LH  A NK  +  L+ +LL AG HLD +N  G++F SL   +GQ + ++INP+ YT LQCLAA  IRR G++Y   L P+L +FV  H
Sbjct:    1 MEFKNGVFNAARDGNLRRLKMLLDHRSRSEVARMVSARTNGATPLVMASKNGHLSCVEYLVERCNADLEQVGSVVFDGETIDGAPPLWCAAAAGHLDVVSYLLGKAANVNATTKTNSTPLRAACFDGHFDIVKYLVEHNADIEVANRHGHTCLMIARYKGHFDIAKYLIDIAANVNRKSVKGNTALHDCAESGSLEIMKLLLDHCAKMDVDSYGMTPLLAASVTGHTHIVEYLISRSDLVSRKDKIDALELLGATYVDKKRDMLGALRFWKMAMSERYEYDLMIYPKPVQQSPIAAYENSVEVMTMQQLDEIISDPDEMRMQALLVRERILGPAHPDTSYYIRYRGAVYADTGNFDRCITLWMYALDMQQKILEPLSPMTQSSFVSFTELFSFMMSEAKNRGR---RVPVVNFCDMMRVFEKCMREVEVGMSQLSKVNNSGCGTVLANGGERDTTYFHRTLVITMHLVCLLTKLGPGLTALQRHTMRKAVYHLVRMDPRGTGGVTPLHLACSRDSSATVGRFPICTFPSLEVVSLLLEVGADPCATDQQGNTPLHTLASNKQCRRELLTSLLAAGAHLDTLNGSGQSFASLRASRGQRLSQLINPLHYTSLQCLAAATIRRHGLHYSTVLHPRLSKFVDQH 638          

BLAST of FEM-1 vs. nr
Match: gi|667673680|gb|AIG62900.1| (feminization-1 [Hyriopsis schlegelii])

HSP 1 Score: 850.121 bits (2195), Expect = 0.000e+0
Identity = 402/631 (63.71%), Postives = 499/631 (79.08%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            ME+R+VV+NAARDGKL RLK+FLDH++KDEV +LV AKTNGATPL++ACRNGH DVV+YL++ C A++EQ GSVTFDGETIEGAPPLWCAAAAGH  IV  L E+G +VN TT TNSTPLRAACFDGH++IV++L+   ADIE+ANRHGHTCLMI+CYKGH  I++YL+E+GADVNRKSVKGNTALHDCAESGS++IMK+LL   A+M+ DAYGMTPLLAASV G+  IVEFLIS+ E   R+  IDALELLGAT+VDKKRDMLG+   W+ AMEER  +G    PK+ I S ISAYEN  EV +L+QL D+ISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGA+YADMG+F+RCI LWMYALDMQQK+LEPLSPMTQSS LSFAELFSFM +E RNR       PV F DI+ VF +A++E   G    +K        DRD+T+FNR L+II+H++CL+ +L  ++S +Q+H  K +VY F++  PRG NG TPLHLAC+R++++VGRYP+C FPS   V LLVE GA+ NA D D NT LH+AA N+P +  ++ +LL  G H+DA N + KT   L++G S++++++P+ Y  LQCLAAQ I +  I Y+G +  +LE F+ MH
Sbjct:    1 MEYRSVVYNAARDGKLSRLKIFLDHKSKDEVSKLVHAKTNGATPLIMACRNGHYDVVKYLVDVCKADIEQVGSVTFDGETIEGAPPLWCAAAAGHLPIVEYLVEKGTDVNKTTLTNSTPLRAACFDGHYDIVKFLVAHNADIEIANRHGHTCLMISCYKGHKDISQYLLELGADVNRKSVKGNTALHDCAESGSIDIMKILLKFDAKMEKDAYGMTPLLAASVAGYAKIVEFLISRAE-CCRQEMIDALELLGATYVDKKRDMLGALTFWRAAMEERFAEGNTKLPKEPIISQISAYENAKEVITLEQLDDLISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGALYADMGNFDRCILLWMYALDMQQKVLEPLSPMTQSSFLSFAELFSFMTTEWRNRPA----HPVGFEDIIAVFLRAIDESERG----RKHLLTVAQTDRDMTHFNRLLVIIMHVLCLMCRLKKNMSDNQLHNFKQTVYRFIRYEPRGTNGFTPLHLACSRETTNVGRYPVCSFPSSSVVELLVEVGANVNAVDVDKNTPLHIAAVNRPCRADVIHSLLKNGAHIDAANKDRKTAMQLIRGMSIYDIVSPLDYMSLQCLAAQKIVKCAIPYKGQVPRRLEAFIEMH 622          

BLAST of FEM-1 vs. nr
Match: gi|1339093476|ref|XP_023707976.1| (protein fem-1 homolog CG6966 isoform X2 [Cryptotermes secundus] >gi|1330914068|gb|PNF33258.1| Protein fem-1-like protein [Cryptotermes secundus])

HSP 1 Score: 842.417 bits (2175), Expect = 0.000e+0
Identity = 404/633 (63.82%), Postives = 492/633 (77.73%), Query Frame = 0

Query:   25 KMEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDR-SPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            +M+F+NV++NAARDGKLRRLKVFLDHR K+EV  LV+AKTNGATPLV+ACRNGH DV EYLIE C A++EQ GSV FDG+TIEGAPPLWCAAAAGH  +VRLL   GA+VNSTT+TNSTPLRAACFDGHFEIV++L++ GADIEVANRHGHTCLMIACYKGH++IA++L+ + A+VNRKSVKGNTALHDCAESGSLEI+KLL+ +GA+MDVD+YGMTPLLAASVTGH HIVE+LI   +LV+R  R+DALELLGAT+VDKKRDM+G+ +LWK+AM++R   G    PK    + ++AY++  E+  L+ L D+++DPD MRMQAL++RERILGPAHPDTSYYIRYRGAVYAD G F RC+ LW YALDMQQ MLEPL+PMTQSSL SF ELFSFMM E    S    R  P  F D+MTVF K+V EV  G   + K P      +RD TY +R L+I LHL CLLT+L+P +S +Q H+V   +Y  +K     R G T LHLAC+ D++ VGRYP CQFPS   V +L+  GAD +++D   N  LH+AA +KP  P LV+ LLD G HLDAVNS G TF  LLKGQ++HE++NP+KYT L CLAA+VI +  I Y+G +   LE FV  H
Sbjct:    4 EMDFKNVIYNAARDGKLRRLKVFLDHRTKEEVLMLVAAKTNGATPLVMACRNGHYDVAEYLIEHCKADIEQLGSVVFDGDTIEGAPPLWCAAAAGHLPVVRLLVRNGASVNSTTRTNSTPLRAACFDGHFEIVKFLVDHGADIEVANRHGHTCLMIACYKGHFRIARFLLGLDANVNRKSVKGNTALHDCAESGSLEILKLLIEHGAKMDVDSYGMTPLLAASVTGHTHIVEYLIKLPDLVSRTERVDALELLGATYVDKKRDMIGALELWKRAMDDRYPIGSTHLPKPNQRNTVAAYDHAREIRDLEGLEDLLADPDEMRMQALVIRERILGPAHPDTSYYIRYRGAVYADAGKFNRCVALWNYALDMQQSMLEPLNPMTQSSLFSFTELFSFMMGEQGRPSTRGRRVPPAAFTDLMTVFKKSVREVEAGVEMLDKIPG----GERDATYLHRVLVISLHLACLLTRLLPDVSSEQRHKVHCFMYRLVKQDVHTRLGCTALHLACSIDAALVGRYPACQFPSSHLVDVLLTVGADVDSRDETGNMPLHLAAMSKPCPPALVRKLLDHGAHLDAVNSAGHTFPQLLKGQAIHEIVNPLKYTTLSCLAARVIVKHNIPYKGIIPVTLEPFVEQH 632          

BLAST of FEM-1 vs. nr
Match: gi|321463161|gb|EFX74179.1| (fem-1-like protein [Daphnia pulex])

HSP 1 Score: 840.877 bits (2171), Expect = 0.000e+0
Identity = 406/638 (63.64%), Postives = 499/638 (78.21%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENR------NRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKG-QSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            ME  +VVFNAARDGKLRRLK FLDHRA++EV  LVSA+TNGATP+++ACRNGH DVVEYL+ERC A++EQ GSVTFDGETIEGAPPLW AAAAGH  IVR L   GA+VN TTKTNSTPLRAACFDGHFEIV+YL++ GAD+EVANRHGHTCLMIACYKGH KIA+YLIE+ ADVNRKSVKGNTALHDCAESGSLEI++LLL++GA+M VD+YGMTPLLAA+V+GH HIVE+++   + V RK +IDALELLGATFVD+KRDMLG+   WKQAM+ R   G    PK    SPI+A+ENT+E  +++QL + +SDPD MRMQALLVRERILG AHP+TSYYIRYRGAVYAD G+F RCI LWMYAL+MQQ MLEPLSPMT SSLLSFAELFSFMM+E         RS PN   P++F D+M V ++AV E+R G L + K  A     +RD+TYF+RTL II+HLI LL K+ P L+  Q H ++ S+Y  ++L+ R RNG T LHLAC RDS+++ RYPIC FPS + +RLL++ GADP + D+D NT+LH+ AQ +P     V  +L  G HLD VN +G++F  LL+  Q +HEV+N +++T L+CL A+VIR+  + + G +   L+ FV  H
Sbjct:    1 MEHSSVVFNAARDGKLRRLKGFLDHRARNEVALLVSARTNGATPMLMACRNGHLDVVEYLVERCGADIEQAGSVTFDGETIEGAPPLWVAAAAGHVHIVRFLVRGGASVNCTTKTNSTPLRAACFDGHFEIVKYLVDHGADMEVANRHGHTCLMIACYKGHLKIARYLIELAADVNRKSVKGNTALHDCAESGSLEILQLLLASGAKMVVDSYGMTPLLAAAVSGHTHIVEYIVHNLDTVGRKEKIDALELLGATFVDRKRDMLGALDYWKQAMQIRYESGVCSLPKPQGQSPIAAFENTLEATTVEQLEEAVSDPDGMRMQALLVRERILGAAHPETSYYIRYRGAVYADTGNFNRCIRLWMYALEMQQSMLEPLSPMTNSSLLSFAELFSFMMAEPALGLMAPQRSPPNI-PPLSFSDVMAVLSRAVREIRAGTLQLSKVAA----AERDLTYFHRTLGIIVHLIFLLAKIQPTLNSLQDHALRKSLYDLVRLNARSRNGYTLLHLACTRDSTTLSRYPICSFPSTEVIRLLLDVGADPESTDHDGNTALHILAQTQPCPSSAVSLVLQRGGHLDQVNLQGRSFAQLLENVQPIHEVVNTLQFTTLKCLCARVIRQHRLLFNGLVPQTLQPFVLKH 633          

BLAST of FEM-1 vs. nr
Match: gi|1228003569|ref|XP_021933670.1| (protein fem-1 homolog CG6966 isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 836.639 bits (2160), Expect = 0.000e+0
Identity = 407/632 (64.40%), Postives = 488/632 (77.22%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDR-SPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            M+ +NV+FNAARDGKLRRLKVFLDHR K+EV  LV+AKTNGATPLV+ACRNGH DV EYLIE C AN EQPGSV FDGETIEGAPPLWCAAAAGH  +VRLL    A VNSTT+TNSTPLRAACFDGHFEIV++L++ GADIEVANRHGHTCLMIACYKGH++IAK+L+ + A+VNRKSVKGNTALHDCAESGS +I+KLL+ +GA+MDVD+YGMTPLLAASVTGH HIVE+LI+   LV+R  R+DALELLGAT+VDKKRDM+G  +LWK+AM++R   G    PK    + ++AY++  E++ L+ L D+++DPD MRMQAL++RERILGPAHPDTSYYIRYRGAVYAD G F RC+ LW YALDMQQ MLEPL+PMTQSSL SF ELFSFMM E    S    R  PV F D+MTVF K+V EV+ G   + K PA     +RD TY +R ++I LHL  LLTKL+P LS +Q HEV   +Y  +K   R R G T LHLAC+RD++ VGRYP CQFPS   V +L+  GAD +++D + N  +H+AA  KP  P L+ TLLD G H DAVNS G +F  LLKGQSVHE++N +KYT L CLAA+ I +  I Y+G +   LE FV  H
Sbjct:    1 MDLKNVIFNAARDGKLRRLKVFLDHRTKEEVLMLVAAKTNGATPLVMACRNGHYDVAEYLIEHCKANTEQPGSVVFDGETIEGAPPLWCAAAAGHLPVVRLLVRNEACVNSTTRTNSTPLRAACFDGHFEIVKFLVDHGADIEVANRHGHTCLMIACYKGHFRIAKFLLSLDANVNRKSVKGNTALHDCAESGSWDILKLLIEHGAKMDVDSYGMTPLLAASVTGHTHIVEYLINLPGLVSRTERVDALELLGATYVDKKRDMIGGLELWKRAMDDRYHVGSTHLPKPRQRNTVAAYDHAKEISDLEGLEDLLADPDEMRMQALVIRERILGPAHPDTSYYIRYRGAVYADAGKFNRCVDLWNYALDMQQSMLEPLNPMTQSSLFSFTELFSFMMGEQGRTSVRGRRVPPVTFTDLMTVFKKSVHEVQAGVEMLDKIPA----GERDATYLHRVVVISLHLGSLLTKLLPDLSLEQQHEVHCLMYKLVKQDIRTRLGCTALHLACSRDATLVGRYPACQFPSPHLVEVLLAVGADVDSQDENGNMPIHLAAMTKPCPPELICTLLDHGAHPDAVNSAGHSFPQLLKGQSVHEIVNLLKYTTLSCLAARTIVKYNIPYKGIVPVTLEAFVQKH 628          

BLAST of FEM-1 vs. nr
Match: gi|918995446|ref|XP_013398307.1| (protein fem-1 homolog C-like [Lingula anatina])

HSP 1 Score: 836.639 bits (2160), Expect = 0.000e+0
Identity = 395/635 (62.20%), Postives = 501/635 (78.90%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKK-TP---ANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            MEF++VV+NAARDGK+ RL++FLD+R K+EV++L+ AKTNGATPL+ A RNGH +VVEYL++ C A +EQ G VTFDGETIEGAPPLWCAAAAGH  + + L E GA VNSTT TNSTPLRAACFDGHFEIV+YL+ER A+IE+ANRHGHTCLMI+CYKGHYKI K+L+ +GADVNRKSVKGNTALHDCAESGS+EIMKLLLS+ ARMD DAYGMTPLLAASVTG   +VE+L+++++ + R+ +IDALELLGAT+VDKKRDMLG+ + W+ A++ER  D K + PK    S ++AYEN VEV + ++L ++ISDPD MRMQALLVRERILGPAHPDTSYYIRYRGAVYADMG+F+RCI LWMYALDMQQKMLEPLSPMTQSS LSFAELFSF MS+ R R       PV F DIM+VF K V E+  G   + K TP   A  + +++D ++FNRTL I++HL+CL++++   LSP+Q    K S+Y  ++L PRG+ G TPLHLAC++D+++VGRYP+C FPS +   LL+E GA     D + NT+LH+AA NKP K  +++ LL+ G HLD  N+ G+T   L+K  ++ +V +P++Y  LQCLAA+VIR + + Y+G +  +LE FV  H
Sbjct:    1 MEFKSVVYNAARDGKVSRLRIFLDNRPKEEVQKLIHAKTNGATPLIAASRNGHSEVVEYLVKHCRAELEQVGQVTFDGETIEGAPPLWCAAAAGHFEVAKFLIEGGAGVNSTTLTNSTPLRAACFDGHFEIVKYLVERKANIEIANRHGHTCLMISCYKGHYKIVKFLLSLGADVNRKSVKGNTALHDCAESGSVEIMKLLLSHDARMDKDAYGMTPLLAASVTGQTKVVEYLLTRSD-IAREEKIDALELLGATYVDKKRDMLGALQYWRMALDERHRDHKSIIPKPTTDSVVAAYENAVEVKTYEELDELISDPDDMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGNFDRCIVLWMYALDMQQKMLEPLSPMTQSSFLSFAELFSFWMSDGRGRPA----HPVEFNDIMSVFEKGVAEIEHGMAYLAKLTPEEKARMLPSEKDGSHFNRTLTIMMHLVCLMSRMKKKLSPEQEFSFKKSIYKLVQLKPRGQKGQTPLHLACSKDTTTVGRYPVCSFPSAEVANLLMEVGASLEDVDVEGNTALHIAAANKPCKLDVMRVLLNHGAHLDMCNASGRTPMQLMKNATIGDVCSPLEYISLQCLAAKVIRTQKLPYKGHVPAKLEDFVEKH 630          

BLAST of FEM-1 vs. nr
Match: gi|762133515|ref|XP_011449960.1| (PREDICTED: protein fem-1 homolog C [Crassostrea gigas] >gi|405954771|gb|EKC22118.1| fem-1-like protein C [Crassostrea gigas])

HSP 1 Score: 823.928 bits (2127), Expect = 0.000e+0
Identity = 390/631 (61.81%), Postives = 494/631 (78.29%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            ME+RNVV+NAARDGK+ RLK+FLDH++KDEV +LV AKTNGATPL++ACRNGH DVV YL+E C A++EQ G VTFDGETIEGAPPLWCAAAAGH+ IV+ L E+G+ VN TT TNSTPLRAACFDGH EIV++L+E  ADIE+ANRHGHTCLMI+CYKGH  IAK+L+E+GADVNRKSVKGNTALHDCAESGSL+IMK+LL   ARM+ DAYGMTPLLAASV G+  IVE+LI++ +   R+ +IDALELLGAT+VDKKRDMLG+   W+ AMEER        PK    S ++AYEN  EV SL+QL ++ SDPD MRMQALLVRERILGPAHPDTSYYIRYRGA+YADMG+F+RCI LWMYALDMQQ +LE LSPMTQSS LSFAELFSFM +E RNR       PV F DIM V  +A++E+  GQ ++ K        +RD+++FN+ LIII+H++CL+ +L   L P++ HE+K  VY  ++++PRG  G TPLHLAC++D+++VGRYP+C FPS +  +LL+E GA+ NA D D N+ LH+AA N+P +  +++TLL  G HLD  N + KT   L++G S++++++P+ Y  LQCL+A+ I +  I Y+G +  +LE F+ MH
Sbjct:    1 MEYRNVVYNAARDGKINRLKIFLDHKSKDEVVKLVHAKTNGATPLIMACRNGHLDVVRYLLELCKADLEQVGCVTFDGETIEGAPPLWCAAAAGHTQIVKYLVEKGSGVNKTTFTNSTPLRAACFDGHIEIVKFLVEHKADIEIANRHGHTCLMISCYKGHKDIAKFLLELGADVNRKSVKGNTALHDCAESGSLDIMKMLLKFNARMEKDAYGMTPLLAASVAGYSKIVEYLITRQD-SERQEKIDALELLGATYVDKKRDMLGAISFWRSAMEERYASPHSPVPKPPSGSEVAAYENAKEVVSLEQLDELASDPDNMRMQALLVRERILGPAHPDTSYYIRYRGALYADMGNFDRCIMLWMYALDMQQSVLEALSPMTQSSFLSFAELFSFMTTEWRNRP----AHPVAFRDIMDVLLRAIKELDQGQNHLMKVAI----PERDLSHFNKLLIIIMHILCLMCRLQKSLKPEEEHELKQVVYKLVQMNPRGTKGYTPLHLACSKDTTNVGRYPVCSFPSAEVAQLLIEVGANVNAVDVDKNSPLHIAAANRPCRSEVIRTLLKNGSHLDMCNGDRKTAMQLIRGMSINDIVSPLNYLSLQCLSARKIVKCNIPYKGHIPQKLESFIMMH 622          

BLAST of FEM-1 vs. nr
Match: gi|1242785648|ref|XP_022332820.1| (protein fem-1 homolog C-like [Crassostrea virginica])

HSP 1 Score: 816.994 bits (2109), Expect = 0.000e+0
Identity = 386/631 (61.17%), Postives = 494/631 (78.29%), Query Frame = 0

Query:   26 MEFRNVVFNAARDGKLRRLKVFLDHRAKDEVKQLVSAKTNGATPLVLACRNGHKDVVEYLIERCYANVEQPGSVTFDGETIEGAPPLWCAAAAGHSSIVRLLAEQGANVNSTTKTNSTPLRAACFDGHFEIVQYLIERGADIEVANRHGHTCLMIACYKGHYKIAKYLIEIGADVNRKSVKGNTALHDCAESGSLEIMKLLLSNGARMDVDAYGMTPLLAASVTGHMHIVEFLISKTELVTRKSRIDALELLGATFVDKKRDMLGSYKLWKQAMEERIVDGKLVYPKQVIASPISAYENTVEVASLDQLVDIISDPDAMRMQALLVRERILGPAHPDTSYYIRYRGAVYADMGHFERCITLWMYALDMQQKMLEPLSPMTQSSLLSFAELFSFMMSENRNRSNPNDRSPVNFCDIMTVFNKAVEEVRMGQLNIKKTPANSVSNDRDITYFNRTLIIILHLICLLTKLMPHLSPDQIHEVKVSVYHFLKLHPRGRNGNTPLHLACARDSSSVGRYPICQFPSLDAVRLLVECGADPNAKDNDSNTSLHVAAQNKPAKPLLVQTLLDAGVHLDAVNSEGKTFESLLKGQSVHEVINPMKYTKLQCLAAQVIRREGINYRGALFPQLERFVAMH 656
            ME+RNVV+NAARDGK+ RLK+FLDH++KDEV +LV AKTNGATPL++ACRNGH DVV+YL+E C A++EQ G VTFDGETIEGAPPLWCAAAAGH+ IV+ L ++G+ VN TT TNSTPLRAACFDGH EIV++L+E  ADIE+ANRHGHTCLMI+CYKGH  IAK+L+E+GADVNRKSVKGNTALHDCAESGSL+IMK+LL   ARMD DAYGMTPLLAASV G+  IVE+LI++ +   ++ +IDALELLGAT+VDKKRDMLG+   W+ AMEER         K +  S ++AYEN  EV SL+QL ++ SDPD MRMQALLVRERILGPAHPDTSYYIRYRGA+YADMG+F+RCI LWMYALDMQQ +LE LSPMTQSS LSFAELFSFM +E RNR       PV F DIM V  +A++E+  GQ ++ K        +RD+++FN+ L+II+H++CL+ +L   L  ++ HE+K  VY  ++++PRG  G TPLHLAC++D+++VGRYP+C FPS +  +LL+E GA+ NA D D N+ LH+AA N+P +  +++TLL  G HLD  N + KT   L++G S++++++P+ Y  LQCL+A+ I +  I Y+G +  +LE F+ MH
Sbjct:    1 MEYRNVVYNAARDGKINRLKIFLDHKSKDEVVKLVHAKTNGATPLIMACRNGHLDVVKYLLELCKADLEQVGCVTFDGETIEGAPPLWCAAAAGHTQIVKYLVDKGSGVNKTTFTNSTPLRAACFDGHIEIVKFLVEHKADIEIANRHGHTCLMISCYKGHKDIAKFLLELGADVNRKSVKGNTALHDCAESGSLDIMKMLLKFSARMDKDAYGMTPLLAASVAGYSKIVEYLITRQD-CDKQEKIDALELLGATYVDKKRDMLGAISFWRSAMEERYSSPHSPVLKPLSGSQVAAYENAKEVVSLEQLDELASDPDNMRMQALLVRERILGPAHPDTSYYIRYRGALYADMGNFDRCIMLWMYALDMQQSVLEALSPMTQSSFLSFAELFSFMTTEWRNRP----AHPVAFRDIMDVLLRAIKELDQGQNHLMKVAI----PERDLSHFNKLLVIIMHILCLMCRLQKSLKTEEEHELKQVVYKLVQMNPRGSKGYTPLHLACSKDTTNVGRYPVCSFPSAEVAQLLIEVGANVNAVDVDKNSPLHIAAANRPCRSEVIRTLLKNGSHLDMCNGDRKTAMQLIRGMSINDIVSPLNYLSLQCLSARKIVKCNIPYKGHIPQKLESFIMMH 622