BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000007660 (supercontig:LSalAtl2s:LSalAtl2s445:12562:17493:1 gene:EMLSAG00000007660 transcript:EMLSAT00000007660 description:"maker-LSalAtl2s445-augustus-gene-0.18")

HSP 1 Score: 286.574 bits (732), Expect = 6.748e-87
Identity = 165/384 (42.97%), Postives = 232/384 (60.42%), Query Frame = 0

Query:  264 EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFG---FAQSVPFLAYS---GCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGG-KLNVEGSNIESLNVPMLR-STMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            E ISNIRTV G   E   +ELY        E  K R     L  G   F  +  F   S   G + YGG+L+ +  L+   +     ++      + Q +    +  K + + SR+F+ +++ PLI       +  ++  G++ F N  F YPTR E  VL+D +L +   +T+ALVG SG GK+T   LL+RFYD+ GG  L ++G NI  L+   LR S +G ++QEPVLF  S+ +NI YG  +   + +EV EAA+ A++  F+ + P GY T++GE+G  +SGGQKQR+AIARAL++NP IL+LDEATSALD ESE++VQEALD   +GRTS+ IAHRLSTI+N D I VI KG ++E GTH  L  +GG+YS+L   Q
Sbjct:  297 ECISNIRTVRGFAMEDAEMELY------SREVDKTRDFNEALGLGIGVFQGASNFFLNSIVLGTITYGGYLMSDDNLNPGQLMSFLVSVQTIQRSITQVSLLFGHLVKGMASGSRIFEYIEKVPLIPISGGVKIPYHSFFGDVEFKNITFSYPTRPEQAVLKDFSLRIPPSKTVALVGTSGGGKTTIAALLERFYDINGGGSLEIDGINIRDLDPSWLRGSAIGYINQEPVLFATSVIENIRYGRPN--ATDNEVYEAAKAAHVDDFVRTFPDGYSTILGERGVTVSGGQKQRIAIARALLKNPPILILDEATSALDAESERIVQEALDKLSKGRTSLVIAHRLSTIKNADVIAVIDKGVMAEIGTHAALKRKGGIYSRLIEQQ 672          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000010048 (supercontig:LSalAtl2s:LSalAtl2s662:116216:125875:1 gene:EMLSAG00000010048 transcript:EMLSAT00000010048 description:"snap_masked-LSalAtl2s662-processed-gene-1.13")

HSP 1 Score: 278.87 bits (712), Expect = 4.184e-83
Identity = 168/417 (40.29%), Postives = 248/417 (59.47%), Query Frame = 0

Query:  241 SNSTGALCSRISSDASAIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLV-----------ENQGLDY--KNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINN----IQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLA-RGGLYSKLWSTQ 639
            + S  AL  + S+  S    +A EA S+I+TV     +   ++ Y E L +  +    R  + G+  G    + + +Y+   +YG  L+            +  + Y   N+  V  ++++G M +GQA+ +   +  A  AAS VF+++   P I S+     R  +      G ++F N  F YP+R  V+VL  L+     G+T+ALVGPSGCGKST IQL+QRFYD   G ++++G +I +L+   LRS +GIV QEPVLF+ S+ +NI  G    ++S   + +  + AN + FI  LP+ YDT+VGEKG  LSGGQKQR+AIARAL+RNP+ILLLDEA+SALD++SE +VQ ALD A++GRT+I +AHRLSTI++ D I V+  G   + GTH  L + + GLY  L + Q
Sbjct:   69 AKSQTALTVKESNAYSKAGSVAEEAFSSIKTVMSFQGQNTEIQRYKENLSEAQKTGILRGLLTGIGGGLMWFIIYSSYAIAFWYGVKLILDDRESCIASPTDCQIRYGPSNLLIVFFSVLMGAMNIGQASPYVEAFAIARGAASSVFQIIQSTPAIKSDYDHLQRQQDKAPPFTGRITFKNVHFEYPSRPTVKVLSGLSFEASPGKTLALVGPSGCGKSTVIQLIQRFYDPSFGVVSIDGEDITTLDPHWLRSHIGIVGQEPVLFEYSIKENITMGLQG-EISEKMIDDTCKAANAYDFIQRLPKKYDTIVGEKGALLSGGQKQRIAIARALIRNPSILLLDEASSALDSQSEFIVQSALDKARKGRTTIIVAHRLSTIRSADAILVMKDGYRVDYGTHESLKSNKTGLYCSLVNAQ 484          

HSP 2 Score: 190.274 bits (482), Expect = 2.584e-51
Identity = 98/200 (49.00%), Postives = 135/200 (67.50%), Query Frame = 0

Query:   68 ERRPSAASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQSKRRLKRQDTILDHPE--EVEELPKIS----MMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGL 261
            ERR S  S  +S+DS  +ED     GSA+G++RV S     R+++R  T     E  E EE+  ++       +L+ENS EW Y+ +G +AS+VMGAS P+YA LFGEVLGVLS+  E AR N   Y + FL+ GI+VG +MF+QI MFS++GE LT ++R  +F +ML QE+ W+D   NSTGALCSR+S+DASA+QG+
Sbjct:  545 ERRHSTGSG-YSEDSLKIEDALDVAGSAIGIARVGS-----RKIRRTSTNFTDNEYLEAEEMKSVNSNVGFFTVLRENSKEWLYIFMGCIASVVMGASMPVYAHLFGEVLGVLSKSIEEARVNSITYSMYFLLVGIIVGFSMFMQIFMFSLSGELLTTKLRIKAFTAMLNQEVGWYDESVNSTGALCSRLSADASAVQGV 738          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000003104 (supercontig:LSalAtl2s:LSalAtl2s174:364234:370462:-1 gene:EMLSAG00000003104 transcript:EMLSAT00000003104 description:"maker-LSalAtl2s174-augustus-gene-3.18")

HSP 1 Score: 204.527 bits (519), Expect = 1.437e-55
Identity = 133/369 (36.04%), Postives = 211/369 (57.18%), Query Frame = 0

Query:  262 AIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPF-LAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSE--STSGLRINNIQ-GNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELL 626
            AI+++ N  TV     E    + Y ++L +   A+ K +    L+  F Q+  F +A S  M      + N  L   ++  V   L   ++ +    +      ++LI    +F+L+     I S   +T    INN++  +++F + +F Y   K +     L+ SV++G+TIA+VG SG GKST ++LL RFY+ Q G + +   NI  + +  LR  + IV Q+ VLF+ ++  NI YG+     S ++V + A+ A I + I S P+GY T VGE+G +LSGG+KQRVAIARA +++  I++ DEATS+LD+ +E ++ +AL     G+T+I IAHRLST+ + D IFV++ G+V E GTH ELL
Sbjct:  223 AIDSLINYETVKYFNNEAYECQQYDKSLLKYETASLKTTESLSLL-NFGQNAIFSVALSAIMMMAAKDISNGALTVGDLIMVNGLLFQLSLPLNFLGSVYREIRQSLIDMQVMFQLMATPSKITSSKGATEINFINNMEDASITFDDVKFGYNIDKNIA--NGLSFSVESGKTIAIVGESGSGKSTLVRLLYRFYEPQLGSIKIGNYNINEVTLDSLRRQISIVPQDCVLFNDTIFHNIKYGN--LNCSDEDVYKVAKLAEIDNAIRSWPQGYLTQVGERGLKLSGGEKQRVAIARAALKDSPIIIFDEATSSLDSITETMIMKALKRVTSGKTAIIIAHRLSTVVHADEIFVLSNGKVIERGTHEELL 586          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000004490 (supercontig:LSalAtl2s:LSalAtl2s2344:77714:79426:-1 gene:EMLSAG00000004490 transcript:EMLSAT00000004490 description:"maker-LSalAtl2s2344-augustus-gene-0.12")

HSP 1 Score: 183.341 bits (464), Expect = 8.514e-51
Identity = 117/312 (37.50%), Postives = 180/312 (57.69%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVG----QATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEAT 568
            LA E I+NIRTV   GKE +    Y E +    +   K + I+   +G       L     M+YGG+LV    +   N+     + IL +  VG      ++F     KAL AA+R+++++DR   +  ++   L +  + G+++F    F YP+R +  +   LNL++++ Q +A+VG     KST   LL R YD   G++ ++G++I  LN   LR+ +GIV QEPVLF  ++ +NI YG   + +  +E+V AA+++N H FI + P  YDT+VGE+G  LSGGQKQRVAIA A+++NP  L+LDEAT
Sbjct:  175 LAQERIANIRTVRAFGKEVQESMAYDEKMSLVLDKGVKEALIQAKFYGMTGXTGNLIILSVMFYGGFLVTQDVITVGNL----TSFILYSGYVGIGLNGVSSFYAEIMKALGAATRIWEIMDRTTHMPLDTGLILPMP-LNGHITFEKVGFSYPSRPDHSIFDGLNLNIESKQILAIVGS----KSTLTSLLLRLYDPNMGRVCIDGTDIRELNTTWLRNQIGIVMQEPVLFSGTIKENILYGTEGK-IEHEEIVSAAKESNAHDFIMNFPDRYDTLVGERGVLLSGGQKQRVAIAXAILKNPQXLILDEAT 476          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000002221 (supercontig:LSalAtl2s:LSalAtl2s1420:21426:31801:1 gene:EMLSAG00000002221 transcript:EMLSAT00000002221 description:"maker-LSalAtl2s1420-augustus-gene-0.12")

HSP 1 Score: 145.206 bits (365), Expect = 6.674e-36
Identity = 84/231 (36.36%), Postives = 136/231 (58.87%), Query Frame = 0

Query:  397 QGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLA 627
            +G +SF N    Y    +  VL++++L ++ G+ I +VG +G GKS+    + R  +   G + ++G +I  L +  LRS + I+ Q+PVLF  SL  N+   D   + +   ++EA   +++  FI SL  G +  V E G  LS GQ+Q + +ARAL+R   +L+LDEAT+A+D E++ ++Q+ + +  +  T ITIAHRL+TI +  +I V+  G   E GT  ELLA
Sbjct: 1317 KGEISFKNYSTRYREGLD-YVLKNVSLDIEGGEKIGIVGRTGAGKSSFTLSMFRIIEPVTGNIIIDGVDITKLGLHQLRSRITIIPQDPVLFSGSLRRNLDPLDEYNETN---IMEAISHSHLKPFIDSLKDGLEYHVSEGGENLSLGQRQLICLARALLRRTRVLILDEATAAVDLETDNLIQKTIRSEFKDSTVITIAHRLNTIMDYSKILVLKNGERVEYGTVAELLA 1543          

HSP 2 Score: 110.538 bits (275), Expect = 4.504e-25
Identity = 71/240 (29.58%), Postives = 125/240 (52.08%), Query Frame = 0

Query:  400 LSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEG----RTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKL 635
            ++   A   + + ++  +L D++L ++ G  +A+VG  G GKS+ +  +    D   G + V GS             +   +Q+  + + SL +NI +  +        +VEA     + S +  LP G +T +GEKG  LSGGQKQRV++ARA+     I LLD+  SA+D+   K + + + ++  G    +T + + H ++ +   D+I V+  GRVSE GT+ ELL + G +++ 
Sbjct:  636 INLEKACLKWESDEDKNILSDVSLDIEVGSLVAVVGTVGSGKSSLLSAILGEMDKVSGLITVRGS-------------IAYGAQQAWVLNTSLKNNILFNKSYDGDKYTRIVEACA---LKSDLDMLPGGDETEIGEKGINLSGGQKQRVSLARAVYSGSDIYLLDDPLSAVDSHVGKHIYDNVISSSSGILKDKTRVMVTHGVTYLPFTDKIIVMKDGRVSEIGTYKELLRQKGAFAEF 859          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000001655 (supercontig:LSalAtl2s:LSalAtl2s128:1836948:1854487:1 gene:EMLSAG00000001655 transcript:EMLSAT00000001655 description:"augustus_masked-LSalAtl2s128-processed-gene-18.4")

HSP 1 Score: 141.739 bits (356), Expect = 7.815e-35
Identity = 88/269 (32.71%), Postives = 146/269 (54.28%), Query Frame = 0

Query:  365 KALIAASRVFKLLDRKPLIDSESTSGLRINNI------QGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNI-AYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELL 626
            K L+A  R +  +DR   I  ES SG  + N+      QG + F + R  Y       VL  +N   +  + I +VG +G GKS+ I  L R      G++ V+G  I  L +   R    ++ Q+P +F  ++  N+  Y  +S Q   D +  +     I+SF +S   G DT++G+ G  LS GQKQ + +ARA++ +  ++ +DEAT+++D E++++++  L  A   +T ITIAHR+ T+ N DRIFV++ G++ E G   +L+
Sbjct: 1182 KELVAVERAYDYIDR---IYEESYSG--VLNMPYNWPNQGVVEFKDIRLRYKDHLPY-VLNKVNFKTKPKEKIGIVGRTGAGKSSLIAALFRLSSFSHGEIMVDGIRIRLLPLIDFRRQFAVIPQDPFIFSGTIRQNLDPYAQHSDQELWDSIKLSYLYNIIYSFGSS---GLDTLIGDGGKSLSIGQKQLLCLARAIITSAKVVFIDEATASVDKETDRLIRNVLKTAFNDKTVITIAHRIETVLNSDRIFVMSNGQIIEEGKPEDLI 1441          

HSP 2 Score: 94.7449 bits (234), Expect = 4.272e-20
Identity = 65/213 (30.52%), Postives = 109/213 (51.17%), Query Frame = 0

Query:  413 KEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTE-SEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVS--ESGTH 622
            K+   LR++++S++  + + ++GP G GK+T +  +    + Q GK++V          P+  S +  V Q P + ++S+ DNI +G+        +V++A      H F   L RG  T+ GEKG  LSGGQK R+A+ARA+ ++  I L+D+  S+LD     KV  E + N  + +T I   H    I + + +  I  G     +  TH
Sbjct:  645 KDCFTLRNIDVSIKRQEFVGVLGPVGSGKTTFLNAILGELEKQSGKISVRD--------PL--SGIAYVQQVPWIQNKSIRDNILFGEMYIHGKYTKVIKAC--CLDHDF-KHLHRGDHTIAGEKGAALSGGQKARIALARAIYQDKDIYLIDDVFSSLDVNVGYKVYTEVMLNLLKRKTRILCTHNPQYINDANIVIKIKDGEFESVKQSTH 844          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000011700 (supercontig:LSalAtl2s:LSalAtl2s812:79332:86643:-1 gene:EMLSAG00000011700 transcript:EMLSAT00000011700 description:"maker-LSalAtl2s812-augustus-gene-0.6")

HSP 1 Score: 141.739 bits (356), Expect = 8.491e-35
Identity = 83/265 (31.32%), Postives = 152/265 (57.36%), Query Frame = 0

Query:  367 LIAASRVFKLLDRKPLIDSESTSGLRINN---IQGNLSFSNARFHYPTRKEVR-VLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLA 627
            ++A  R+ +  +++     E    L++N+   ++G ++  N    Y  RK++  VLRD+N+ +  G  I +VG +G GKST    L R  ++  G++ ++G +I+ + +  LRS + I+ Q+P LF  +L  N+   D        E++E+ + A +  F+     G D ++ E G  +S GQ+Q + +ARAL+R   +L++DEAT+A+D+E++K++Q+A+ +     T +TIAHRL T+ +  +I VI  G + E  +   LL+
Sbjct: 1715 IVAVERIKEYSNKESEAPWELEEDLKLNSSWPLKGEITIKNLSLKY--RKDLDWVLRDINVHISPGDKIGIVGRTGSGKSTLALALFRLLEVNSGQITIDGVDIKKMGLHTLRSKLTIIPQDPTLFSGTLRLNL---DPFGLFKDMELIESLKHAYLQDFLKE--DGLDKVIAENGENISLGQRQLICLARALLRKNKVLIMDEATAAVDSETDKLIQKAIKDNFRDSTVLTIAHRLQTVLDYSKIIVIDSGCIIEYDSPKNLLS 1972          

HSP 2 Score: 110.538 bits (275), Expect = 5.338e-25
Identity = 74/244 (30.33%), Postives = 125/244 (51.23%), Query Frame = 0

Query:  417 VLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPM-LRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEAL---DNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPNATMLVDRAKIVE 656
            + +D+++ V  G  IA++GP G GKS+ I  L     L       +G+N      P+ +  ++    Q   + + S+ DNI +G    +   DEV++A    ++ S +   P G  T +GEKG  LSGGQKQR+++ARA+     I  LD+  + +D+   K + E +   +    G+T I + H LS +  VD I+ +  G ++E GT+ EL+ +   +S+L S    +   T   D  K++E
Sbjct: 1163 LFKDISMDVNRGNLIAIIGPVGSGKSSLISAL-----LGEMLFTSKGTN------PVEIHGSVAYTPQNAWMQNVSVKDNILFGRKFNKNWYDEVLKAC---SLESDMEXFPSGDSTEIGEKGINLSGGQKQRISLARAVYSQSDIYFLDDPLAXVDSHVAKHLFEEVIGSNGLLRGKTRILVTHNLSFLHLVDEIYFLKDGEITEKGTYKELMEKNCDFSELISNHA-NNKITNQSDSEKVME 1391          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000003611 (supercontig:LSalAtl2s:LSalAtl2s197:49356:53594:-1 gene:EMLSAG00000003611 transcript:EMLSAT00000003611 description:"augustus_masked-LSalAtl2s197-processed-gene-0.4")

HSP 1 Score: 140.198 bits (352), Expect = 2.251e-34
Identity = 78/229 (34.06%), Postives = 130/229 (56.77%), Query Frame = 0

Query:  397 QGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGEL 625
            QG + F N    Y    ++ VL+ +N ++  G+ + +VG +G GKS+    L R  +   G++ ++G +I +L +  LR ++ ++ QEP+LF  +L  N+   D     S  ++       ++  +I  L  G +  + E G  LS GQ+Q + +ARAL+R+  IL+LDEAT+A+D E+++ +QE +       T +TIAHRL TI + D+I V+ KG V E G+  EL
Sbjct: 1156 QGKVEFKNYSLRYKANSDL-VLKKINFTIFGGERVGIVGRTGAGKSSLSVALFRIVEAANGQILIDGKDISTLGLHDLRESLTVIPQEPLLFSGTLRSNL---DPENAFSDHDIWNTLGSVHLKEYIRDLKDGLNHSISEGGLNLSSGQRQLMCLARALLRSCKILILDEATAAVDWETDEKIQETIRCYFPSCTILTIAHRLKTIIDYDKILVLDKGTVIEFGSPNEL 1380          

HSP 2 Score: 101.293 bits (251), Expect = 3.456e-22
Identity = 66/229 (28.82%), Postives = 115/229 (50.22%), Query Frame = 0

Query:  404 NARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEG----RTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLAR 628
            +  F + T      L ++NL ++ G   A+VG  G GKS+ I  +      + GK+ ++G              +  V QE  + + SL +NI +G    +   ++V+E      +   ++ LP G +T +GE+G  LSGGQ+QR+++ARA+  +  + + D   SALD++  K V   + N   G    +T + + H +S + +V  I V+ KG +   GT+ ELL +
Sbjct:  557 DGNFKWSTEDSENTLSNINLRIKHGSLTAIVGTVGSGKSSLISAILGEMVKKSGKVRIKGQ-------------IAYVPQESWMQNTSLKNNILFGKPYDEEWYNQVLEYC---CLTYDLSLLPAGDETEIGERGINLSGGQRQRISLARAIYSDADVYIFDNPLSALDSQVGKSVFNNVLNNNTGIIKDKTRLLVTHGISYLPHVHHIAVMLKGEIIHEGTYQELLEK 769          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000008248 (supercontig:LSalAtl2s:LSalAtl2s498:229862:245360:1 gene:EMLSAG00000008248 transcript:EMLSAT00000008248 description:"maker-LSalAtl2s498-snap-gene-2.25")

HSP 1 Score: 135.191 bits (339), Expect = 9.955e-33
Identity = 81/236 (34.32%), Postives = 136/236 (57.63%), Query Frame = 0

Query:  397 QGNLSFSNARFHYPTRKEVR-VLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNI----AYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLA 627
             G + F N +  Y  RK +  VL+ +N ++Q G+ I +VG +G GKS+    L R  +   G + ++G NI  L +  LRS + I+ Q+P+LF  SL  N+    A+ D    ++++        +++ SF+ +L  G +  V E G  LS GQ+Q + +ARA++R   IL+LDEAT+A+D E++ ++Q  + +  +  T +TIAHR++TI + +RI V+  G ++E      LLA
Sbjct: 1277 HGVIKFDNYKTRY--RKGLDLVLKGINCTIQKGEKIGIVGRTGAGKSSLTLALFRIIEPSEGSIYIDGENIRFLGLGKLRSRITIIPQDPILFSGSLRMNLDPFEAFADRDIWIALE-------YSHLKSFVWNLGDGLNFSVLEGGRNLSVGQRQLICLARAILRKTQILVLDEATAAIDLETDDLIQSTIRSEFKDSTVLTIAHRINTIMDSNRIMVLDAGTIAEFDDPQNLLA 1503          

HSP 2 Score: 102.834 bits (255), Expect = 1.174e-22
Identity = 67/230 (29.13%), Postives = 116/230 (50.43%), Query Frame = 0

Query:  403 SNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEG----RTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLAR 628
             N    + T + +  L D++L ++ G  +A+VG  G GKS+ +  +    ++  G +NV+G  I +              Q+P L + +L +NI +G    +     V++A     +   +  LP G  T +GE+G  LSGGQK RV++AR    N  I +LD+  SA+D    + + E + ++Q G    +T I + H    +   D+I V+  G +SESGT+ +L+ +
Sbjct:  620 CNVSLSWETDEGLNTLEDIDLDIEKGSLVAIVGQVGSGKSSLLSGILGEMEIVRGSINVDGQTIYA-------------PQQPWLQNETLRNNILFGKRFNKKLYRRVIDACA---LSPDLDMLPAGDLTEIGERGINLSGGQKARVSLARCTXNNGDIYILDDPLSAVDVHVGRHLFENVISSQTGLLKNKTRIFVTHGAIFLPQTDKIIVMKNGVISESGTYRDLIEK 833          

BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000002016 (supercontig:LSalAtl2s:LSalAtl2s1361:43077:48755:-1 gene:EMLSAG00000002016 transcript:EMLSAT00000002016 description:"augustus_masked-LSalAtl2s1361-processed-gene-0.2")

HSP 1 Score: 126.331 bits (316), Expect = 5.822e-30
Identity = 74/217 (34.10%), Postives = 115/217 (53.00%), Query Frame = 0

Query:  421 LNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLAR-GGLYSKLW 636
             +L +   Q + + G SG GKST +  + R   +  G + + G NI ++ +  LR  +  + Q+ VLF  ++  N+   D     S + +     KA+    + + P G DT V E G   S GQ+Q + I +AL+R P +++LDE+TSALD   E  +   L  A E  T I++AHRLS I   +R+ V+ +GR+ E G   ELL +  G +S LW
Sbjct: 1670 FSLEIPYRQKVGICGRSGSGKSTLLMGIVRLSRVLQGSITINGININAIPLSRLRKFVITIPQDAVLFSGTIRSNL---DPENDFSDELIWSTLDKADCGKTVRNFPDGLDTCVTENGDNFSLGQRQELNIVKALLRRPRVVILDESTSALDPNREVALHNTLLEAFEDSTLISVAHRLSNIVEYERVLVMGEGRILEDGNPKELLKKPMGFFSALW 1883          

HSP 2 Score: 98.5969 bits (244), Expect = 2.421e-21
Identity = 68/221 (30.77%), Postives = 106/221 (47.96%), Query Frame = 0

Query:  404 NARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTE-SEKVVQEALDNA--QEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGT 621
            N  F +P + E  VL+ +NL ++ G    ++GPSG GK+  I  L    D   G +          NVP    T+ ++ Q P L + ++ DNI  G   ++    +V+ A    ++ + I  LP G DT +GE+G  LSGGQ+QR+AIAR L        +D   S+LD++ +  V +E +     +  RT      RL  +   D I  +  G +   GT
Sbjct:  732 NGTFAWP-KCETNVLQSINLEIKTGSLTIVIGPSGSGKTALISSLIEEMDRITGSV--------KWNVP---DTVALLGQRPWLLNTTIKDNILLGRPFKEKRYKKVIAAC---DLQTDIDLLPHGDDTEIGERGVLLSGGQRQRLAIARCLYSKSYCTFMDAPFSSLDSKITSHVFEEGVLKILLKRRRTVFLTTERLDFLHRADHIVALKDGMIKAQGT 937          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|238054374|sp|P08183.3|MDR1_HUMAN (RecName: Full=Multidrug resistance protein 1; AltName: Full=ATP-binding cassette sub-family B member 1; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243)

HSP 1 Score: 525.013 bits (1351), Expect = 5.063e-170
Identity = 280/613 (45.68%), Postives = 391/613 (63.78%), Query Frame = 0

Query:   98 LSRVVSDRQSKRRLKRQDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS--EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLG-KEQKFLE----LYLEALQ-------------------QPHEAAKKRSHIRGLIFG----FAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            L R  S R+S R  + QD  L   E ++E +P +S  RI+K N +EWPY +VG+  +I+ G   P +AI+F +++GV +  +DPE+ R+N   + +LFL  GI+  +  FLQ   F  AGE LT R+R M F SML+Q+++WFD P N+TGAL +R+++DA+ ++G                                        +AI  +  ++ ++G   K++K LE    +  EA++                   Q  +   + S  +  IFG    F Q++ + +Y+GC  +G +LV ++ + +++V  V  A++ G M VGQ ++FAP+Y KA I+A+ +  ++++ PLIDS ST GL  N ++GN++F    F+YPTR ++ VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   GK+ ++G  I+ LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR VS +E+V AA++ANIH+FI SLP  Y T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I V   GRV E GTH +LLA+ G+Y  + S Q
Sbjct:  662 LIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQ 1274          

HSP 2 Score: 360.147 bits (923), Expect = 3.946e-108
Identity = 188/380 (49.47%), Postives = 255/380 (67.11%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G ++K LE Y + L++      K++    +  G A  + + +Y+   +YG  LV +       V  V  ++++G   VGQA+     +  A  AA  +FK++D KP IDS S SG + +NI+GNL F N  F YP+RKEV++L+ LNL VQ+GQT+ALVG SGCGKST +QL+QR YD   G ++V+G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+MDE+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE VVQ ALD A++GRT+I IAHRLST++N D I     G + E G H EL+   G+Y KL + QT
Sbjct:  253 VAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYG--RENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQT 630          

HSP 3 Score: 61.2326 bits (147), Expect = 2.318e-8
Identity = 42/155 (27.10%), Postives = 75/155 (48.39%), Query Frame = 0

Query:  128 PKISMMRILKENSSEWP---YMLVGLLASIVMGASTPIYAILFGEVLGVLS-----EDPESA----------------RENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAI 258
            P +S+  + +   S W    YM+VG LA+I+ GA  P+  ++FGE+  + +     ED  S                  E++  Y   +   G  V +A ++Q+S + +A      ++RK  F ++++QE+ WFD   +  G L +R++ D S I
Sbjct:   32 PTVSVFSMFR--YSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFD--VHDVGELNTRLTDDVSKI 182          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|239938877|sp|P21447.3|MDR1A_MOUSE (RecName: Full=Multidrug resistance protein 1A; AltName: Full=ATP-binding cassette sub-family B member 1A; AltName: Full=MDR1A; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 3)

HSP 1 Score: 517.309 bits (1331), Expect = 3.275e-167
Identity = 278/617 (45.06%), Postives = 388/617 (62.88%), Query Frame = 0

Query:   94 SAVGLSRVVSDRQSKRRLKRQDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSED--PESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLG-KEQKFLE----LYLEALQ-------------------QPHEAAKKRSHIRGLIFG----FAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            S   L R  S R+S      QD  L   E ++E +P  S  RILK NS+EWPY +VG+  +I+ G   P ++++F +V+GV +    PE+ R+N   + +LFL+ GI+  +  FLQ   F  AGE LT R+R M F+SML+Q+++WFD P N+TGAL +R+++DA+ ++G                                        +AI  +  ++ ++G   K++K LE    +  EA++                   Q  +   + +  +  +FG    F Q++ + +Y+ C  +G +LV  Q + ++NV  V  A++ G M VGQ ++FAP+Y KA ++AS + +++++ P IDS ST GL+ N ++GN+ FS   F+YPTR  + VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G  I+ LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR VS +E+V AA++ANIH FI SLP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y  + S Q
Sbjct:  654 SGSSLIRRRSTRKSICGPHDQDRKLSTKEALDEDVPPASFWRILKLNSTEWPYFVVGIFCAIINGGLQPAFSVIFSKVVGVFTNGGPPETQRQNSNLFSLLFLILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1270          

HSP 2 Score: 360.533 bits (924), Expect = 2.146e-108
Identity = 190/380 (50.00%), Postives = 255/380 (67.11%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G ++K LE Y   L++      K++    +  G A  + + +Y+   +YG  LV ++      V  V  ++++G   VGQA+     +  A  AA  VFK++D KP IDS S SG + +NIQGNL F N  F YP+RKEV++L+ LNL V++GQT+ALVG SGCGKST +QL+QR YD   G ++++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+MDE+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE VVQ ALD A+EGRT+I IAHRLST++N D I     G + E G H EL+   G+Y KL  TQT
Sbjct:  249 VAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG--REDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQT 626          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|126302568|sp|P21439.2|MDR3_HUMAN (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 3)

HSP 1 Score: 508.064 bits (1307), Expect = 1.772e-163
Identity = 259/594 (43.60%), Postives = 366/594 (61.62%), Query Frame = 0

Query:  127 LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSE-DPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLGKEQK----------------------------FLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNV-------EGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPN 644
            +P +S +++LK N +EWPY +VG + +I  G   P ++++F E++ +    D    ++    + ++FL  GI+     FLQ   F  AGE LT R+R M+F++ML+Q+M+WFD   NSTGAL +R+++DA+ +QG                                        +A+  I  ++ +AG  K  K                            F  +Y+E L  P+  + +++HI G+ F  +Q+  + +Y+GC  +G +L+ N  + +++V  V  A++ G + +G A++FAP+Y KA ++A+ +F L +R+PLIDS S  GL+ +  +GN++F+   F+YPTR  V VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + V       +G   + LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR VS DE+V AA+ ANIH FI +LP  Y+T VG+KGTQLSGGQKQR+AIARAL+R P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I V   GRV E GTH +LLA+ G+Y  + S Q  + N
Sbjct:  692 VPPVSFLKVLKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVFVDFGFQLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTQN 1285          

HSP 2 Score: 353.214 bits (905), Expect = 1.494e-105
Identity = 182/377 (48.28%), Postives = 250/377 (66.31%), Query Frame = 0

Query:  264 EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            EA+  IRTV   G + K LE Y + L+   E   K++    +  G A  + + +Y+   +YG  LV ++     N   V  ++++G   VGQA      +  A  AA  +F ++D  P IDS S  G + ++I+GNL F++  F YP+R  V++L+ LNL VQ+GQT+ALVG SGCGKST +QL+QR YD   G +N++G +I + NV  LR  +G+VSQEPVLF  ++A+NI YG  +  V+MDE+ +A ++AN + FI  LP+ +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE  VQ ALD A+EGRT+I IAHRLST++N D I     G + E G+H EL+ + G+Y KL + QT
Sbjct:  258 EALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGN--VTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQT 632          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|1170903|sp|Q08201.1|MDR3_RAT (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 2; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 2; AltName: Full=P-glycoprotein 3)

HSP 1 Score: 505.753 bits (1301), Expect = 1.019e-162
Identity = 261/611 (42.72%), Postives = 376/611 (61.54%), Query Frame = 0

Query:  103 SDRQSKRRLKRQDTILDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESAREN-VAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLGKEQK----------------------------FLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPN 644
            S R  + RL  +   LD       +P +S +++L+ N +EWPY +VG L +I  GA  P ++I+  E++ +     ++ ++     + ++FL  G+      FLQ   F  AGE LT R+R M+F++ML+Q+M+WFD   NSTGAL +R+++DA+ +QG                                        +A+  I  ++ +AG  K  K                            F  +Y+E L  P+  + +++HI G+ F  +Q+  + +Y+GC  +G +L+ N  + +K+V  V  A++LG + +G A++FAP+Y KA ++A+ +F L +R+PLIDS S  G+  +  +G+++F+   F+YPTR  V VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G   + LNV  LR+ +GIVSQEP+LFD S+A NIAYGDNSR VS DE+V AA++ANIH FI +LP+ Y+T VG+KGTQLSGGQKQR+AIARAL+R P +LLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y  + + Q  + N
Sbjct:  672 SSRAHQNRLDVETNELD-----ANVPPVSFLKVLRLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAIFGPGDDTVKQQKCNMFSLVFLGLGVHSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAKNIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQN 1277          

HSP 2 Score: 345.125 bits (884), Expect = 9.127e-103
Identity = 193/446 (43.27%), Postives = 279/446 (62.56%), Query Frame = 0

Query:  264 EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPNATMLVDRAKIVELLVWTRTMSI--KTWTGK----ATKDVLAAALAHFMARSDLDEDIAELEASPP 703
            EA+  IRTV   G + K LE Y + L+   +   K++    +  G A  + + +Y+   +YG  LV ++     N   V  ++++G   VGQA      +  A  AA  +F ++D  P IDS S  G + ++I+GNL FS+  F YP+R  +++L+ LNL V++GQT+ALVG SGCGKST +QLLQR YD   G ++++G +I + NV  LR  +G+VSQEPVLF  ++A+NI YG  +  V+MDE+ +A ++AN + FI  LP+ +DT+VG++G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE  VQ ALD A+EGRT+I IAHRLST++N D I     G + E G+H EL+ + G+Y +L + QT   + + ++     VEL        +    W  +    +TK  L ++ AH   ++ LD +  EL+A+ P
Sbjct:  255 EALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFPNARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYGRGN--VTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQT---SGSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAH---QNRLDVETNELDANVP 692          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|338817954|sp|P21440.2|MDR3_MOUSE (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 2; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 2; AltName: Full=P-glycoprotein 3)

HSP 1 Score: 505.368 bits (1300), Expect = 1.298e-162
Identity = 261/610 (42.79%), Postives = 380/610 (62.30%), Query Frame = 0

Query:  107 SKRRLK--RQDTILDHPEEVE-ELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSE-DPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLGKEQK----------------------------FLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPN 644
            +K+ LK   Q+ + +   E++  +P +S +++LK N +EWPY +VG + +I  GA  P ++I+  E++ +    D    ++    + ++FL  G++     FLQ   F  AGE LT R+R M+F++ML+Q+M+WFD   NSTGAL +R+++DA+ +QG                                        +A+  I  ++ +AG  K  K                            F  +Y+E L  P+  + +++HI G+ F  +Q+  + +Y+GC  +G +L+ N  + +K+V  V  A++LG + +G A++FAP+Y KA ++A+ +F L +R+PLIDS S  GL  +  +G+++F+   F+YPTR  V VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G   + LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR V  DE+V AA++ANIH FI +LP+ Y+T VG+KGTQLSGGQKQR+AIARAL+R P +LLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y  + + Q  + N
Sbjct:  666 TKKSLKSPHQNRLDEETNELDANVPPVSFLKVLKLNKTEWPYFVVGTVCAIANGALQPAFSIILSEMIAIFGPGDDAVKQQKCNMFSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVPHDEIVRAAKEANIHPFIETLPQKYNTRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQN 1275          

HSP 2 Score: 350.132 bits (897), Expect = 1.725e-104
Identity = 181/377 (48.01%), Postives = 251/377 (66.58%), Query Frame = 0

Query:  264 EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            EA+  IRTV   G + K LE Y + L+   +   K++    +  G A  + + +Y+   +YG  LV ++     N   V  ++++G   VGQA      +  A  AA  +F ++D  P IDS S  G + +NI+GNL FS+  F YP+R  +++L+ LNL V++GQT+ALVG SGCGKST +QLLQR YD   GK++++G +I + NV  LR  +G+VSQEPVLF  ++A+NI YG  +  V+MDE+ +A ++AN + FI  LP+ +DT+VG++G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE  VQ ALD A+EGRT+I IAHRLSTI+N D I     G + E G+H EL+ + G+Y +L + QT
Sbjct:  255 EALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGN--VTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQT 629          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|126930|sp|P23174.1|MDR3_CRIGR (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 3)

HSP 1 Score: 497.664 bits (1280), Expect = 1.235e-159
Identity = 259/612 (42.32%), Postives = 372/612 (60.78%), Query Frame = 0

Query:  103 SDRQSKRRLKRQDTILDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSE-DPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLGKEQK----------------------------FLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPNA 645
            S R    RL      +D  E    +P +S +++LK N +EWPY +VG + +IV GA  P  +I+  E++ +    D    ++    + ++FL  G++     FLQ   F  AGE LT R+R M+F++ML+Q+M+WFD   NSTGAL +R+++D + +QG                                        +A+  I  ++ +AG  K  K                            F  +Y+E L +P+  + + +HI G+ F  +Q+  + +Y+GC  +G +L+ N  + +++V  V  A++ G + +G A++FAP+Y KA ++A+ +F L +R+PLIDS S  GL  +  +G+++F+   F+YPTR  + VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G   + LN+  LR+ +GIVSQEPVLFD S+A+NIAYGDNSR VS DE+V AA+ ANIH FI +LP+ Y T VG+KGTQLSGGQKQR+AI RAL+R P +LLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y  + + Q  + N+
Sbjct:  675 SSRAHHHRLD-----VDADELDANVPPVSFLKVLKLNKTEWPYFVVGTVCAIVNGALQPAISIILSEMIAIFGPGDDAVKQQKCNLFSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDYKNSTGALSTRLATDRAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVSGIVEMKMLAGNAKRDKKALEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHEPYRNSVQMAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANMPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNIQWLRAQLGIVSQEPVLFDCSIAENIAYGDNSRVVSQDEIVRAAKAANIHPFIETLPQKYKTRVGDKGTQLSGGQKQRLAIRRALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVNIQAGAQNS 1281          

HSP 2 Score: 348.591 bits (893), Expect = 5.846e-104
Identity = 197/446 (44.17%), Postives = 280/446 (62.78%), Query Frame = 0

Query:  264 EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPNATMLVDRAKIVELLVWTRT--MSIKTWTG----KATKDVLAAALAHFMARSDLDEDIAELEASPP 703
            EA+  IRTV   G + K LE Y + L+   +   K++    +  G A  + + +Y+   +YG  LV ++     N   V  ++++G   VGQA      +  A  AA  +F ++D  P IDS S  G + ++I+GNL FS+  F YP+R  +++L+ LNL VQ+GQT+ALVG SGCGK+T +QLLQR YD   G ++++G +I + NV  LR  +G+VSQEPVLF  ++A+NI YG  +  V+M+E+ +A ++AN + FI  LP+ +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE  VQ ALD A+EGRT+I IAHRLST++N D I     G + E G+H EL+ + G+Y KL + QT   + + ++ +   VEL        M+   W       +TK  L ++ AH   R D+D D  EL+A+ P
Sbjct:  258 EALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLDFSDVHFSYPSRANIKILKGLNLKVQSGQTVALVGNSGCGKTTTLQLLQRLYDPTEGTISIDGQDIRNFNVRYLREIIGVVSQEPVLFSTTIAENIRYGRGN--VTMEEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMQKEGVYFKLVNMQT---SGSQILSQEFEVELSEEKAADGMTPNGWKSHIFRNSTKKSLKSSRAHHH-RLDVDAD--ELDANVP 695          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|262527527|sp|O95342.2|ABCBB_HUMAN (RecName: Full=Bile salt export pump; AltName: Full=ATP-binding cassette sub-family B member 11)

HSP 1 Score: 493.041 bits (1268), Expect = 1.411e-157
Identity = 266/606 (43.89%), Postives = 372/606 (61.39%), Query Frame = 0

Query:  102 VSDRQSKRRLKRQDTILDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS-EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG----------------------------------------LAIEAISNIRTVAGLG-KEQKFLEL-----------------------YLEA----LQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWST 638
            V D +S     R+D  +   EEVE  P   + RILK ++ EWPYMLVG + + V G  TP+YA LF ++LG  S  D E  R  +   C+LF+  G V     FLQ   F+ +GE LT R+RK  F +ML Q++AWFD   NS GAL +R+++DAS +QG                                        LA+   +  R + G   ++++ LE+                       ++EA    L++P + A ++++I G  F FAQ + F+A S    YGG+L+ N+GL +  VF+V  A++L    +G+A ++ P+Y KA I+A+R F+LLDR+P I   +T+G + +N QG + F + +F YP+R + +VL  L++S+  GQT+A VG SGCGKST IQLL+RFYD   GK+ ++G + + +NV  LRS +GIVSQEPVLF  S+ DNI YGDN++++ M+ V+ AA++A +H F+ SLP  Y+T VG +G+QLS G+KQR+AIARA+VR+P ILLLDEATSALDTESEK VQ ALD A+EGRT I IAHRLSTIQN D I V+A+G V E GTH EL+A+ G Y KL +T
Sbjct:  714 VVDHKSTYEEDRKDKDIPVQEEVEPAP---VRRILKFSAPEWPYMLVGSVGAAVNGTVTPLYAFLFSQILGTFSIPDKEEQRSQINGVCLLFVAMGCVSLFTQFLQGYAFAKSGELLTKRLRKFGFRAMLGQDIAWFDDLRNSPGALTTRLATDASQVQGAAGSQIGMIVNSFTNVTVAMIIAFSFSWKLSLVILCFFPFLALSGATQTRMLTGFASRDKQALEMVGQITNEALSNIRTVAGIGKERRFIEALETELEKPFKTAIQKANIYGFCFAFAQCIMFIANSASYRYGGYLISNEGLHFSYVFRVISAVVLSATALGRAFSYTPSYAKAKISAARFFQLLDRQPPISVYNTAGEKWDNFQGKIDFVDCKFTYPSRPDSQVLNGLSVSISPGQTLAFVGSSGCGKSTSIQLLERFYDPDQGKVMIDGHDSKKVNVQFLRSNIGIVSQEPVLFACSIMDNIKYGDNTKEIPMERVIAAAKQAQLHDFVMSLPEKYETNVGSQGSQLSRGEKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQVALDKAREGRTCIVIAHRLSTIQNADIIAVMAQGVVIEKGTHEELMAQKGAYYKLVTT 1316          

HSP 2 Score: 313.538 bits (802), Expect = 3.897e-91
Identity = 180/407 (44.23%), Postives = 256/407 (62.90%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYK--NVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPNATMLVDRAKIVELLVWTRTMS 665
            +A E IS++RTVA  G E++ +E Y + L        ++  + G   GF   + FL Y+   +YG  LV ++G +Y    + ++  ++I+G + +G A+     +     AA+ +F+ +DRKP+ID  S  G +++ I+G + F N  FHYP+R EV++L DLN+ ++ G+  ALVGPSG GKST +QL+QRFYD   G + V+G +I SLN+  LR  +GIV QEPVLF  ++A+NI YG      +M+++V+AA++AN ++FI  LP+ +DT+VGE G Q+SGGQKQRVAIARAL+RNP ILLLD ATSALD ESE +VQE L   Q G T I++AHRLST++  D I     G   E GTH ELL R G+Y  L + Q+    A    D     E  +  RT S
Sbjct:  280 VADEVISSMRTVAAFGGEKREVERYEKNLVFAQRWGIRKGIVMGFFTGFVWCLIFLCYALAFWYGSTLVLDEG-EYTPGTLVQIFLSVIVGALNLGNASPCLEAFATGRAAATSIFETIDRKPIIDCMSEDGYKLDRIKGEIEFHNVTFHYPSRPEVKILNDLNMVIKPGEMTALVGPSGAGKSTALQLIQRFYDPCEGMVTVDGHDIRSLNIQWLRDQIGIVEQEPVLFSTTIAENIRYG--REDATMEDIVQAAKEANAYNFIMDLPQQFDTLVGEGGGQMSGGQKQRVAIARALIRNPKILLLDMATSALDNESEAMVQEVLSKIQHGHTIISVAHRLSTVRAADTIIGFEHGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTFS 683          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|126924|sp|P21448.2|MDR1_CRIGR (RecName: Full=Multidrug resistance protein 1; AltName: Full=ATP-binding cassette sub-family B member 1; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243)

HSP 1 Score: 448.743 bits (1153), Expect = 3.860e-141
Identity = 215/379 (56.73%), Postives = 282/379 (74.41%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            +A EAI N RTV  L +EQKF  +Y ++LQ P+  A K++H+ G+ F F Q++ + +Y+ C  +G +LV  + + ++NV  V  A++ G M VGQ ++FAP+Y KA ++AS +  ++++ P IDS ST GL+ N ++GN+ F+   F+YPTR ++ VL+ LNL V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G  +  LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR VS DE+  AA++ANIH FI SLP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y  + S Q
Sbjct:  893 IATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVARELMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIMIIEKVPSIDSYSTGGLKPNTLEGNVKFNEVVFNYPTRPDIPVLQGLNLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEVNQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1271          

HSP 2 Score: 357.066 bits (915), Expect = 4.545e-107
Identity = 187/380 (49.21%), Postives = 254/380 (66.84%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G ++K LE Y   L++      K++    +  G A  + + +Y+   +YG  LV ++      V  V  A+++G   +GQA+     +  A  AA  +F ++D KP IDS S +G + +NI+GNL F N  F YP+RK+V++L+ LNL VQ+GQT+ALVG SGCGKST +QLLQR YD   G ++++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+MDE+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE VVQ ALD A+EGRT+I IAHRLST++N D I     G + E G H EL+   G+Y KL  TQT
Sbjct:  250 VAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFAVLIGAFSIGQASPNIEAFANARGAAYEIFNIIDNKPSIDSFSKNGYKPDNIKGNLEFKNIHFSYPSRKDVQILKGLNLKVQSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG--RENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADIIAGFDGGVIVEQGNHEELMREKGIYFKLVMTQT 627          

HSP 3 Score: 141.739 bits (356), Expect = 2.150e-33
Identity = 68/150 (45.33%), Postives = 101/150 (67.33%), Query Frame = 0

Query:  114 QDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSE--DPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG 260
            QD  L   E ++E +P IS  RILK NSSEWPY +VG+  +IV GA  P ++I+F +V+GV +   D E+ R +   + +LFL+ G++  +  FLQ   F  AGE LT R+R M F+SML+Q+++WFD P N+TGAL +R+++DA  ++G
Sbjct:  675 QDRKLSTKEALDEDVPPISFWRILKLNSSEWPYFVVGIFCAIVNGALQPAFSIIFSKVVGVFTRNTDDETKRHDSNLFSLLFLILGVISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDNPKNTTGALTTRLANDAGQVKG 824          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|126928|sp|P21449.2|MDR2_CRIGR (RecName: Full=Multidrug resistance protein 2; AltName: Full=P-glycoprotein 2)

HSP 1 Score: 443.351 bits (1139), Expect = 4.225e-139
Identity = 212/372 (56.99%), Postives = 281/372 (75.54%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLY 632
            +A EAI N RTV  L +EQKF  +Y ++LQ P+  A K++H+ G+ F F Q++ + +Y+ C  +G +LV +Q + ++NV  V  A++ G +  G A++FAP+Y KA ++AS + +++++ P IDS ST GL+ N ++GN+ F+   F+YPTR ++ VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G  I+ LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR VS DE+  AA++ANIH FI SLP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y
Sbjct:  894 IATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAHQIMTFENVMLVFSAVVFGAIAAGNASSFAPDYAKAKVSASHIIRIMEKIPSIDSYSTRGLKPNWLEGNVKFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIY 1265          

HSP 2 Score: 348.977 bits (894), Expect = 3.717e-104
Identity = 183/383 (47.78%), Postives = 250/383 (65.27%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPN---YNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G + K LE Y + L++      K++    +  G A  + + +Y+   +YG  LV +       V  V  +++ GT  +G     APN   +  A  AA  +FK++D +P IDS ST G + +++ GNL F N  F YP+R  +++L+ LNL VQ+GQT+ALVG SGCGKST +QLLQR YD   G ++++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+MDE+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE VVQ ALD A+EGRT+I IAHRLST++N D I     G + E G H EL+   G+Y +L   QT
Sbjct:  252 VAEEVLAAIRTVIAFGGQNKELERYNKNLEEAKNVGIKKAVTANISIGIAYLLVYASYALAFWYGTSLVLSNEYSVGQVLTVFFSILFGTFSIGH---IAPNIEVFANARGAAYEIFKIIDNEPSIDSFSTQGHKPDSVMGNLEFKNVHFSYPSRSGIKILKGLNLKVQSGQTVALVGKSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG--RENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHEELMKEKGIYCRLVMMQT 629          

HSP 3 Score: 134.035 bits (336), Expect = 5.555e-31
Identity = 62/155 (40.00%), Postives = 104/155 (67.10%), Query Frame = 0

Query:  125 EELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS--EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGLAIEAISNI-RTVAGLG 276
            E++P +S   ILK N +EWPY++VG+L +++ G   P+++I+F  ++GV +  +DP++ ++N   + + FLV G++  +  F Q   F  AGE LT R+R M F+SML+Q+++WFD   NSTGAL +R++SDA+ ++G     ++ I + VA LG
Sbjct:  688 EDVPLVSFWGILKLNITEWPYLVVGVLCAVINGCMQPVFSIVFSGIIGVFTRDDDPKTKQQNCNLFSLFFLVMGMICFVTYFFQGFTFGKAGEILTKRLRYMVFKSMLRQDISWFDDHRNSTGALTTRLASDAANVKGAMSSRLAGITQNVANLG 842          

BLAST of P-glycoprotein vs. SwissProt
Match: gi|126927|sp|P06795.1|MDR1B_MOUSE (RecName: Full=Multidrug resistance protein 1B; AltName: Full=ATP-binding cassette sub-family B member 1B; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243)

HSP 1 Score: 442.58 bits (1137), Expect = 9.331e-139
Identity = 210/372 (56.45%), Postives = 279/372 (75.00%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLY 632
            +A EAI N RT+  L +EQKF  +Y ++LQ P+  A K++H+ G+ F F Q++ + +Y+ C  +G +LV  Q + ++NV  V  A++ G M  G  ++FAP+Y KA ++AS + +++++ P IDS ST GL+   ++GN+ F+  +F+YPTR  + VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G  I+ LNV  LR+ +GIVSQEP+LFD S+A+NIAYGDNSR VS +E+V AA++ANIH FI SLP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  G+V E GTH +LLA+ G+Y
Sbjct:  894 IATEAIENFRTIVSLTREQKFETMYAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQQLMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEGLKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIY 1265          

HSP 2 Score: 355.91 bits (912), Expect = 1.371e-106
Identity = 188/383 (49.09%), Postives = 255/383 (66.58%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPN---YNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G +QK LE Y + L++      K++    +  G A  + + +Y+   +YG  LV +       V  V  +++LGT  +G     APN   +  A  AA  +FK++D +P IDS ST G + ++I GNL F N  F+YP+R EV++L+ LNL V++GQT+ALVG SGCGKST +QL+QR YD   G ++++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+MDE+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE VVQ ALD A+EGRT+I IAHRLST++N D I     G + E G H EL+   G+Y KL  TQT
Sbjct:  252 VAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGEVLTVFFSILLGTFSIGH---LAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPDSIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG--REDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQT 629          

HSP 3 Score: 140.584 bits (353), Expect = 5.382e-33
Identity = 68/158 (43.04%), Postives = 105/158 (66.46%), Query Frame = 0

Query:  106 QSKRRLKRQDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS--EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG 260
            +S  R + Q+  L   E V+E +P +S  RIL  N SEWPY+LVG+L +++ G   P++AI+F  ++GV S  +D E+ R+N   + + FLV G++  +  F Q   F  AGE LT R+R M F+SML+Q+++WFD   NSTG+L +R++SDAS+++G
Sbjct:  668 RSVHRKQDQERRLSMKEAVDEDVPLVSFWRILNLNLSEWPYLLVGVLCAVINGCIQPVFAIVFSRIVGVFSRDDDHETKRQNCNLFSLFFLVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKG 825          

BLAST of P-glycoprotein vs. nr
Match: gi|586962492|gb|AHK05634.1| (ATP-binding cassette transporter sub-family B member 1 [Tigriopus japonicus])

HSP 1 Score: 674.855 bits (1740), Expect = 0.000e+0
Identity = 334/452 (73.89%), Postives = 377/452 (83.41%), Query Frame = 0

Query:  192 FLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARG-GLYSKLWSTQTLS 642
            F    I +GLAM+ Q  +  V   ++   +  + F++ +        + + ++ A              LAIEAISNIRTVAGLG+E+ F ELYL AL+QPH  AKKRSH+RGLIFGFAQSVPF AYSGCM+YGGWLVENQ LDYKNVFKVAEALILGTMMVGQATAFAPNYNKAL+AA+RVFKLLDRKP ID+   +GLRIN+IQGN++FS A FHYPTRKEVRVLR+LNL+VQAGQTIALVGPSGCGKSTCIQLLQRFYDL  G L VEG NI+SLNVP LRS MGIVSQEPVLFDR+LA+NIAYGDNSR  SMDEVV+AAR+ANIHSFI+SLP  YDT+VGEKGTQLSGGQKQRVAIARAL+RNPA+LLLDEATSALDTESEKVVQEALD AQ+GRTSITIAHRLSTIQNV+RIFVI+KGRV E+GTH ELLAR  GLY+KLW +QTLS
Sbjct:  922 FFTLTISIGLAMYYQWQLGLVTSVFIPFVLVALYFQTKMIMGSDSVQKEAFASSA-------------KLAIEAISNIRTVAGLGREKTFEELYLNALRQPHMDAKKRSHVRGLIFGFAQSVPFFAYSGCMFYGGWLVENQDLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALLAAARVFKLLDRKPKIDANDATGLRINDIQGNITFSQAGFHYPTRKEVRVLRELNLAVQAGQTIALVGPSGCGKSTCIQLLQRFYDLHKGVLTVEGQNIQSLNVPQLRSRMGIVSQEPVLFDRTLAENIAYGDNSRTASMDEVVDAARQANIHSFISSLPLKYDTLVGEKGTQLSGGQKQRVAIARALIRNPAVLLLDEATSALDTESEKVVQEALDKAQKGRTSITIAHRLSTIQNVNRIFVISKGRVVEAGTHNELLARKEGLYAKLWGSQTLS 1360          

HSP 2 Score: 339.732 bits (870), Expect = 7.103e-98
Identity = 179/392 (45.66%), Postives = 253/392 (64.54%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQG-------------LDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            +A E +S +RTV   G + K +E +   L    +A   R  + G+  GF   + + +Y+   +YG  L+ +                D  ++  V  ++++G M VGQAT +   ++ A  AA+++F ++DR P IDS ST+G       GNL+F +  F+YP+RK+V++L+ + L +  G+T+ALVG SGCGKST IQL+QRFYD   G + + G ++  LN+  LR  +GIV QEPVLF  ++A+NI YG +   ++  ++ +A + AN +SFI SLP+ YDT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDT+SE VVQ ALD A+ GRT+I +AHRLSTI+  D+I     GRV+E GTHGEL+   G+Y  L S Q
Sbjct:  285 VAEEVLSAVRTVVAFGGQTKEVERFENNLHDAKKAGIMRGLLTGIGGGFMWLIIYASYALAFWYGVKLIMDDTEDCFEDILHCDPRYDASSLLVVFFSVLMGAMNVGQATPYVEAFSVARGAAAQIFDIIDRVPEIDSSSTAGEHPEKGAGNLTFRDVFFNYPSRKDVKILKGMTLDINKGETVALVGASGCGKSTVIQLVQRFYDPLSGSIMLNGKDLRQLNLSALRERIGIVGQEPVLFGCTIAENIRYGRDG--INDSDIEQACKDANAYSFIQSLPKKYDTLVGERGAQLSGGQKQRIAIARALVRNPDILLLDEATSALDTQSEGVVQAALDKARRGRTTIMVAHRLSTIRTADKIVAFEDGRVAEIGTHGELMKMEGVYYGLVSAQ 674          

HSP 3 Score: 298.516 bits (763), Expect = 3.300e-83
Identity = 143/197 (72.59%), Postives = 173/197 (87.82%), Query Frame = 0

Query:   67 SERRPSAASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQSKRRLKR---QDTILDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG 260
            SER+ S ASS+ SDDS T+ED+GHAVGSAVG SRV S + S  + ++    DT L+ P+E  +LPK+SM+RILK NS EWPYML+GLLAS++MGAS P+YAILFGEVLGVLSEDP SAR+NV+YYCILFL+TG+VVG+AMFLQISMF++AGE+LTLRMRK++FE+ML+QEMAWFD PSNSTGALC+RISSDASAIQG
Sbjct:  715 SERKMSVASSILSDDSVTMEDIGHAVGSAVGFSRVPSLQASFYKRQKGSFTDTPLESPDE--DLPKVSMIRILKANSKEWPYMLIGLLASVIMGASMPVYAILFGEVLGVLSEDPVSARDNVSYYCILFLITGMVVGIAMFLQISMFTLAGEHLTLRMRKLAFEAMLRQEMAWFDLPSNSTGALCTRISSDASAIQG 909          

BLAST of P-glycoprotein vs. nr
Match: gi|406654432|gb|AFS49708.1| (P-glycoprotein [Tigriopus japonicus])

HSP 1 Score: 674.085 bits (1738), Expect = 0.000e+0
Identity = 334/452 (73.89%), Postives = 377/452 (83.41%), Query Frame = 0

Query:  192 FLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARG-GLYSKLWSTQTLS 642
            F    I +GLAM+ Q  +  V   ++   +  + F++ +        + + ++ A              LAIEAISNIRTVAGLG+E+ F ELYL AL+QPH  AKKRSH+RGLIFGFAQSVPF AYSGCM+YGGWLVENQ LDYKNVFKVAEALILGTMMVGQATAFAPNYNKAL+AA+RVFKLLDRKP ID+   +GLRIN+IQGN++FS A FHYPTRKEVRVLR+LNL+VQAGQTIALVGPSGCGKSTCIQLLQRFYDL  G L VEG NI+SLNVP LRS MGIVSQEPVLFDR+LA+NIAYGDNSR  SMDEVV+AAR+ANIHSFI+SLP  YDT+VGEKGTQLSGGQKQRVAIARAL+RNPA+LLLDEATSALDTESEKVVQEALD AQ+GRTSITIAHRLSTIQNV+RIFVI+KGRV E+GTH ELLAR  GLY+KLW +QTLS
Sbjct:  901 FFTLTISIGLAMYYQWQLGLVTSVFIPFVLVALYFQTKMIMGSDSVQKEAFASSA-------------KLAIEAISNIRTVAGLGREKTFEELYLNALRQPHMDAKKRSHVRGLIFGFAQSVPFFAYSGCMFYGGWLVENQDLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALLAAARVFKLLDRKPKIDANDATGLRINDIQGNITFSQAGFHYPTRKEVRVLRELNLAVQAGQTIALVGPSGCGKSTCIQLLQRFYDLHKGVLTVEGQNIQSLNVPQLRSRMGIVSQEPVLFDRTLAENIAYGDNSRTASMDEVVDAARQANIHSFISSLPLKYDTLVGEKGTQLSGGQKQRVAIARALIRNPAVLLLDEATSALDTESEKVVQEALDKAQKGRTSITIAHRLSTIQNVNRIFVISKGRVVEAGTHNELLARKEGLYAKLWGSQTLS 1339          

HSP 2 Score: 338.191 bits (866), Expect = 1.971e-97
Identity = 210/619 (33.93%), Postives = 326/619 (52.67%), Query Frame = 0

Query:  127 LPKISMMRILKENSSEWPYML-VGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCI----------------------------LFL----------------VTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQ-------GLAI-----------------------------------------EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQG-------------LDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            LP +++ R+ +  SS   +++ + ++A+I+ G S P+  ILFG++     ++      NV   C+                             F+                + G+V  +  ++ ++  + A E    ++R +  +++L+Q++ W+D  ++ TG   SR++ D + +Q       G+ I                                         +     R +   GK     E  L A++   +A   R  + G+  GF   + + +Y+   +YG  L+ +                D   +  V  ++++G M VGQAT +   ++ A  AA+++F ++DR P IDS ST+G       GNL+F +  F+YP+RK+V++L+ + L +  G+T+ALVG SGCGKST IQL+QRFYD   G + + G ++  LN+  LR  +GIV QEPVLF  ++A+NI YG +   ++  ++ +A + AN +SFI SLP+ YDT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDT+SE VVQ ALD A+ GRT+I +AHRLSTI+  D+I     GRV+E GTHGEL+   G+Y  L S Q
Sbjct:   44 LPPVAIHRLFRFASSRDLFLIFLAIVAAIIGGCSMPVMIILFGDLANTFVQNDL----NVTQICVGIPLCCDDTPAINLDLPNCNVTEEDLGNFFINMNFLEQITTFAQGTALIGLVNFIMSYIFVTCLNHAAECQVFKIRGLFLKAILRQDIGWYD--THQTGDFASRMTEDLNKVQEGIGEKIGMFIFFATIFIASLINAFVHGWELTLVILSVMPVLVIATAIIAGSQTYLTARELKAYGKAGSVAEEVLSAVRTV-KAGIMRGLLTGIGGGFMWLIIYASYALAFWYGVKLIMDDTEDCFEDILHCDPRYDASGLLVVFFSVLMGAMNVGQATPYVEAFSVARGAAAQIFDIIDRVPEIDSSSTAGEHPEKGAGNLTFRDVFFNYPSRKDVKILKGMTLDINKGETVALVGASGCGKSTVIQLVQRFYDPLSGSIMLNGKDLRQLNLSALRERIGIVGQEPVLFGCTIAENIRYGRDG--INDSDIEQACKDANAYSFIQSLPKKYDTLVGERGAQLSGGQKQRIAIARALVRNPDILLLDEATSALDTQSEGVVQAALDKARRGRTTIMVAHRLSTIRTADKIVAFEDGRVAEIGTHGELMKMEGVYYGLVSAQ 653          

HSP 3 Score: 298.13 bits (762), Expect = 3.634e-83
Identity = 143/197 (72.59%), Postives = 173/197 (87.82%), Query Frame = 0

Query:   67 SERRPSAASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQSKRRLKR---QDTILDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQG 260
            SER+ S ASS+ SDDS T+ED+GHAVGSAVG SRV S + S  + ++    DT L+ P+E  +LPK+SM+RILK NS EWPYML+GLLAS++MGAS P+YAILFGEVLGVLSEDP SAR+NV+YYCILFL+TG+VVG+AMFLQISMF++AGE+LTLRMRK++FE+ML+QEMAWFD PSNSTGALC+RISSDASAIQG
Sbjct:  694 SERKMSVASSILSDDSVTMEDIGHAVGSAVGFSRVPSLQASFYKRQKGSFTDTPLESPDE--DLPKVSMIRILKANSKEWPYMLIGLLASVIMGASMPVYAILFGEVLGVLSEDPVSARDNVSYYCILFLITGMVVGIAMFLQISMFTLAGEHLTLRMRKLAFEAMLRQEMAWFDLPSNSTGALCTRISSDASAIQG 888          

BLAST of P-glycoprotein vs. nr
Match: gi|1192754474|ref|XP_015927598.2| (multidrug resistance protein 1-like [Parasteatoda tepidariorum])

HSP 1 Score: 595.119 bits (1533), Expect = 0.000e+0
Identity = 310/600 (51.67%), Postives = 395/600 (65.83%), Query Frame = 0

Query:  110 RLKRQDTILDHPE--EVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNST---------------GALCSRISSDASAIQGL-----------------------------------------------------AIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            R+ R  +    PE  E EEL   S  R+LK  S EWPY+LVG +A+++MG  TPIY ILFG++LGVL+E  +    ++++Y +LF+   I  G+A FLQ  MFS+AGE LT R+R M F +++ Q+M+WFD+P+NS                GA  SRIS+   A+  L                                                     AIEAI +IRTVA L +E  F   +  AL +PH+ ++K S +RG+ FGFAQS+P  AY+  MYYG  L+    L Y ++FKV EA+ILGT MVGQA AFAP+Y KA +AA R+FKLLD KP +D  ST+G  + +++G + F N RF+YP+R  V++LR LNL VQ GQT+ALVG SGCGKSTCIQL++RFYD   G + ++ + ++ +NV  LRS +G+VSQEPVLF  SLA+NIAYGDNSR V+MDE++EAA++ANIHSFIASLP GYDT VG+KG QLSGGQKQR+AIARAL+RNP ILLLDEATSALD ESEKVVQEALD A  GRT + IAHRL+TIQN D I VI KG+V E GTH ELL R G Y  L++TQ
Sbjct:  792 RISRMSSFAFKPENKENEELEP-SKTRLLKMCSPEWPYILVGAIAALIMGLFTPIYGILFGDILGVLAEQNDQILTDISFYSLLFVAMAICSGIASFLQTFMFSIAGEKLTGRLRVMVFTTIISQDMSWFDQPANSVGSLCTRLTSDASSVQGATGSRISTALQALSTLIAAIVIGLWYNYKIGIVVLCFIPLVLVATYFESRVISGQMLNEKSGTEFASKVAIEAIESIRTVASLHQEDSFYMKFRNALYEPHKKSRKNSQVRGVTFGFAQSIPSFAYATSMYYGSILIAEGTLTYPDLFKVIEAVILGTSMVGQAVAFAPDYQKAKLAAVRIFKLLDLKPTVDVFSTAGRFLEDVKGYIRFRNVRFNYPSRHRVKILRGLNLEVQPGQTVALVGSSGCGKSTCIQLIERFYDAHRGHVMLDDTCVKDMNVSNLRSHIGLVSQEPVLFSYSLAENIAYGDNSRTVTMDEIIEAAKQANIHSFIASLPLGYDTPVGDKGAQLSGGQKQRIAIARALLRNPKILLLDEATSALDAESEKVVQEALDKASSGRTCLVIAHRLTTIQNADVIMVIHKGQVVEKGTHQELLNRKGRYYSLYTTQ 1390          

HSP 2 Score: 337.421 bits (864), Expect = 6.696e-97
Identity = 181/390 (46.41%), Postives = 259/390 (66.41%), Query Frame = 0

Query:  257 AIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLV-ENQGL---DYK--NVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            A   +A E +S+IRTV   G E+K ++ Y + L   ++   KR  +  +         +L Y+   +YG  L+ E +G    +YK   +  V   +ILG M VGQ   +   +  A  AA +VF +++RKP IDS S SG    ++ GN++  +  F+YP R +V +L+ L+L +++G+T+ALVGPSGCGKST +QL+QRFYD   G + ++ ++++ LNV  LR  +G+V QEPVLF  S+A+NI  G      S +E+  AA+ AN+H+FI +LP  Y+T+VGE+GTQLSGGQKQR+AIARAL++NP ILLLDEATSALDTESE VVQ ALD A +GRT+I +AHRLSTI+  D+I  ++ G V+E GTH EL+A+ G+Y +L + QT
Sbjct:  352 AAGAVAEEVLSSIRTVVSFGGEKKEIDRYNKCLIPANQKGIKRGLLTSIGASLIWVCIYLGYALAFWYGVKLIFEGRGQPNPEYKPSTLIIVFFNVILGAMGVGQTAPYFEAFAIAKGAAGKVFSIIERKPPIDSSSKSGKFPQSLVGNITLKDVHFNYPARPDVTILKGLSLKIKSGETVALVGPSGCGKSTIVQLVQRFYDADRGTVFIDENDVQELNVGWLRDHIGLVGQEPVLFSTSIAENIRLGKPG--ASQEEIENAAKLANVHNFIDALPLKYETLVGERGTQLSGGQKQRIAIARALIKNPRILLLDEATSALDTESEAVVQAALDKASQGRTTIIVAHRLSTIRTADKIVALSNGIVNEMGTHDELMAKKGIYYQLVTAQT 739          

HSP 3 Score: 65.0846 bits (157), Expect = 4.750e-7
Identity = 38/154 (24.68%), Postives = 76/154 (49.35%), Query Frame = 0

Query:  125 EELPKISMMRILKENSS-EWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSED------------------PESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQ 259
            E+ P +S + + + +S+ +   M+  ++ ++V GA+ P   +LFG +L    +                    E+   +VA  C+   + G +V    ++ +S FS+A      ++R M   S+L+Q+++WFD  ++ TG   SR++ D + IQ
Sbjct:  135 EQTPSVSFLSLFRYSSNLDKLLMITAIIIAVVTGAAMPTIMVLFGNILDSFVDHHRLHNNLTALSGNGTNITTETFLHDVADLCMYICIIGAIVFCFNYIFVSFFSMAAANQAFKIRCMFMRSVLRQDISWFD--THQTGDFASRLTGDLNRIQ 286          

BLAST of P-glycoprotein vs. nr
Match: gi|675364005|gb|KFM56907.1| (Multidrug resistance protein 1, partial [Stegodyphus mimosarum])

HSP 1 Score: 593.578 bits (1529), Expect = 0.000e+0
Identity = 301/577 (52.17%), Postives = 386/577 (66.90%), Query Frame = 0

Query:  131 SMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNST---------------GALCSRISSDASAIQGL-----------------------------------------------------AIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            S  R++K  + EWPY+LVG +A+++MG  TP+Y I+FG++LG+L+E  ES   +++YY ++FL   +  G+A FLQ  MFSVAGE LT R+RKM F +++ Q+++WFD P N                 GA  SRIS+   A+  L                                                     AIEAI +IRTVA L KE+ F   +  AL  PH+ ++K S IRG+ FGFAQS+P  AY+  MYYG  LV    L Y ++FKV EA+ILGT MVGQA AFAP+Y KA +AA R+FKLLD KP ID  ST+G  +++++G + F N RF+YP+R  V++LR LNL ++ GQT+ALVG SGCGKSTCIQLL+RFYD Q G + ++ + ++ +N+  LRS +G+VSQEPVLF  SLA+NIAYGDNSR+V M E++ AAR+ANIH+FIASLP+GYDT VG+KG QLSGGQKQR+AIARAL+RNP ILLLDEATSALD ESEKVVQEALD A  GRT + IAHRLSTIQN D I VI++GRV E GTH ELL + GLY  L +TQ
Sbjct:   87 SQTRLIKMCAPEWPYILVGSIAALIMGIFTPVYGIVFGDILGILAEQSESILNDISYYSLVFLAMAVSAGIACFLQTFMFSVAGEKLTSRLRKMVFTTIISQDISWFDDPKNGVGSLCTRLTSDASSVQGATGSRISTALQALSTLIAAIVIGLWFNYKIGIVVLCFVPLVLLATYFESRVISGHMLSEKSGTEQASKVAIEAIDSIRTVASLHKEEAFYIQFRNALFGPHKKSRKNSQIRGVTFGFAQSIPSFAYACSMYYGSVLVAEGELSYPDLFKVIEAVILGTAMVGQAVAFAPDYQKAKVAAVRIFKLLDMKPSIDIFSTAGRVLDDVKGYIRFKNVRFNYPSRPRVKILRGLNLEIEPGQTVALVGSSGCGKSTCIQLLERFYDAQQGDVMLDDACVKDMNISNLRSHIGLVSQEPVLFSYSLAENIAYGDNSRKVEMHEIIAAARQANIHNFIASLPQGYDTPVGDKGAQLSGGQKQRIAIARALLRNPKILLLDEATSALDAESEKVVQEALDKASSGRTCLVIAHRLSTIQNADTIIVISRGRVVEKGTHQELLNKKGLYYNLHNTQ 663          

BLAST of P-glycoprotein vs. nr
Match: gi|1228402083|ref|XP_021960053.1| (multidrug resistance protein 1A-like [Folsomia candida] >gi|1215260374|gb|OXA46696.1| Multidrug resistance protein 1 [Folsomia candida])

HSP 1 Score: 591.267 bits (1523), Expect = 0.000e+0
Identity = 305/593 (51.43%), Postives = 391/593 (65.94%), Query Frame = 0

Query:  115 DTILDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSEDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQ-------GLAIEAISNIRTVAGLGKEQK---------FLELYLEA------LQQPHEAAKKRS----------------------------------------------HIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            + ++D  +   E P   MMR++K NS EW  + VG+++SI+ GA  P YAILFGE + +L+   + A  N     ILFLV GI  G AMFLQ+ MF++AGE LT R+RK +F +MLKQE+ W+D P+N+ GALC+R+S DA+ +Q       G+ +++I+ I    GL    K         F+   + A      L   H+  +K++                                              H+RG +FGFAQSVPF AY+  MYYGG+LVEN+ L +  VFKVAEALI GTMMVGQ  AFAPNYNKA ++ +R+ +LLDRKP IDS    GL+I N  G++ F    F YPTR   R+LR L+  V+ G+++ALVGPSGCGKSTCIQL+ R+YD   G +  +  ++ SLNV  LRS M IV+QEP LFDR++A NIAYGDNSR V M+E+++AA++ANIHSFIASLP GY+T VGE GTQLSGGQKQRVAI+RALVR P ++LLDEATSALDTESE+VVQ ALD A EGRTSITIAHRLS+IQNVDRI VI +G + ESGTH ELLA+ GLY +LW+ Q
Sbjct:  731 EHLMDEKDLANEAP---MMRLIKMNSPEWKQIAVGIVSSILQGALLPCYAILFGEYMKILTYPDDDAERNANLLAILFLVLGIGAGGAMFLQMFMFALAGEALTARLRKDTFHAMLKQELGWYDDPNNNVGALCARLSGDAAHVQGATGSRVGIIVQSIATIAIAVGLAMYYKPTLGLVAMVFVPFVIAATYFQMLLMMGHDLGEKKAIDEASKIAVQAVGNIRTVASLGKQKSFVQTFNDILKSSHAKIVKMAHLRGAVFGFAQSVPFFAYATVMYYGGYLVENEDLAFDKVFKVAEALIFGTMMVGQTMAFAPNYNKAKVSGARILRLLDRKPAIDSSPGVGLQIRNSSGSVLFDRVAFAYPTRPTARILRSLSYGVEPGKSVALVGPSGCGKSTCIQLILRYYDPLSGTVKFDEQDVASLNVDALRSQMAIVAQEPALFDRTIAQNIAYGDNSRVVPMEEIIDAAKQANIHSFIASLPAGYETRVGEMGTQLSGGQKQRVAISRALVRKPKLILLDEATSALDTESEQVVQAALDAASEGRTSITIAHRLSSIQNVDRILVINQGELYESGTHSELLAKQGLYFRLWNMQ 1320          

HSP 2 Score: 330.872 bits (847), Expect = 8.639e-95
Identity = 180/390 (46.15%), Postives = 255/390 (65.38%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLV----------ENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLR-INNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            +A EA++N++TV       K +E Y + L Q   AAKKR  I     G    + + +Y+   +YG  L+          E+     + +  V  ++++G   +GQ+ ++   +     +A+ ++ ++DR P IDS S  G +     +G LSF N  F YP+R +V++L++ NL+V++GQT+ALVG SGCGKST +QLLQRFYD   G++ V+G +I++L++  LRS +G+VSQEPVLF+ ++ +NI YG     V++ EV EA R++N H FI  LP GY+T+VG++G QLSGGQKQR+AIARALVRNPAILLLDEATSALDT+SE VVQ ALD A++GRT++ +AHRLSTI+  D +  I  G V E GTH ELLA  GLY  L   Q
Sbjct:  291 VAEEALTNVKTVMAFSGSDKEIERYEDGLIQAKAAAKKRGAISAFGVGLMWFIIYASYALAFWYGTKLILEGRPKLCRGEDPEYTAETMLIVFFSVLMGAFNIGQSASYFEAFAVGRGSAAVIYSIIDRIPEIDSYSEEGKKPAQRSRGRLSFRNINFQYPSRPDVQILKNFNLNVESGQTVALVGHSGCGKSTVLQLLQRFYDPIQGQVCVDGEDIKNLHLGWLRSQIGVVSQEPVLFNTTILENIQYG--REDVTLKEVEEACRQSNAHGFICKLPNGYETVVGDRGAQLSGGQKQRIAIARALVRNPAILLLDEATSALDTQSESVVQAALDRARQGRTTLIVAHRLSTIRGADLVVAINDGDVVEKGTHKELLAAKGLYHGLVLAQ 678          

BLAST of P-glycoprotein vs. nr
Match: gi|1126403068|ref|XP_019670494.1| (multidrug resistance protein 1 isoform X2 [Felis catus])

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 285/549 (51.91%), Postives = 394/549 (71.77%), Query Frame = 0

Query:   94 SAVGLSRVVSDRQSKRRLKRQDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS--EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            S   L R  S R+S    + QD  L   E ++E +P +S  RILK N +EWPY +VG+  +I+ G   P ++++F  ++GV +  EDPE+ R+N   + +LFLV GI+  +  FLQ   F  AGE LT R+R M F SML+Q+++WFD P N+TGAL +R+++DA+ ++G+A EAI N RTV  L +EQKF  +Y ++LQ P+  + +++HI G+ F   Q++ + +Y+GC  +G +LV ++ +D+++V  V  A++ G M VGQ ++FAP+Y KA ++A+ V  ++++ PLIDS ST GL  N ++GN++F+   F+YPTR ++ VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G  I+ LNV  LR+ MGIVSQEP+LFD S+ +NIAYGDNSR VS +E+V AA++ANIH FI +LP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I V   G+V E GTH +LLA+ G+Y  + S Q
Sbjct:  658 SGSSLIRRRSTRKSIHASQGQDRKLGTKENLDEHVPPVSFWRILKLNITEWPYFVVGIFCAIINGGLQPAFSVIFSRIIGVFTRVEDPETKRQNSNIFSLLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGISFSITQAMMYFSYAGCFRFGAYLVAHEFMDFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKIPLIDSYSTEGLMPNTLEGNVTFNEVMFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLIDGKEIKHLNVQWLRAHMGIVSQEPILFDCSIGENIAYGDNSRVVSQEEIVRAAKEANIHPFIETLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPRILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1206          

HSP 2 Score: 357.451 bits (916), Expect = 3.680e-105
Identity = 184/380 (48.42%), Postives = 255/380 (67.11%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G ++K LE Y + L++      K++    +  G A  + + +Y+   +YG  LV +       V  V  ++++G   VGQA+     +  A  AA  +FK++D KP IDS S +G + +NI+GNL F N  F YP+RKEV++L+ LNL VQ+GQT+ALVG SGCGKST +QL+QR YD   G ++++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+M+E+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALD+ESE VVQ ALD A++GRT+I +AHRLSTI+N D I     G + E G H EL+   G+Y KL + QT
Sbjct:  253 VAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGIAFLLIYASYALAFWYGTSLVLSHEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKNGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENICYG--RENVTMEEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEAVVQVALDKARKGRTTIVVAHRLSTIRNADVIAGFDDGVIVEKGNHDELMKEEGIYFKLVTMQT 630          

BLAST of P-glycoprotein vs. nr
Match: gi|1111104319|ref|XP_019298733.1| (PREDICTED: multidrug resistance protein 1 isoform X4 [Panthera pardus])

HSP 1 Score: 587.415 bits (1513), Expect = 0.000e+0
Identity = 285/549 (51.91%), Postives = 394/549 (71.77%), Query Frame = 0

Query:   94 SAVGLSRVVSDRQSKRRLKRQDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS--EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            S   L R  S R+S    + QD  L   E ++E +P +S  RILK N +EWPY +VG+  +I+ G   P ++++F  ++GV +  EDPE+ R+N   + +LFLV GI+  +  FLQ   F  AGE LT R+R M F SML+Q+++WFD P N+TGAL +R+++DA+ ++G+A EAI N RTV  L +EQKF  +Y ++LQ P+  + +++HI G+ F   Q++ + +Y+GC  +G +LV ++ +D+++V  V  A++ G M VGQ ++FAP+Y KA ++A+ V  ++++ PLIDS ST GL  N ++GN++F+   F+YPTR ++ VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFYD   G + ++G  I+ LNV  LR+ MGIVSQEP+LFD S+ +NIAYGDNSR VS +E+V AA++ANIH FI +LP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I V   G+V E GTH +LLA+ G+Y  + S Q
Sbjct:  689 SGSSLIRRRSTRKSIHASQGQDRKLGTKENLDEHVPPVSFWRILKLNITEWPYFVVGIFCAIINGGLQPAFSVIFSRIIGVFTRVEDPETKRQNSNIFSLLFLVLGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGIATEAIENFRTVVSLTREQKFEYMYAQSLQVPYRNSLRKAHIFGISFSITQAMMYFSYAGCFRFGAYLVAHEFMDFQDVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSAAHVIMIIEKIPLIDSYSTEGLMPNTLEGNVTFNEVMFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLIDGKEIKHLNVQWLRAHMGIVSQEPILFDCSIGENIAYGDNSRVVSQEEIVRAAKEANIHPFIETLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPRILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1237          

HSP 2 Score: 357.451 bits (916), Expect = 6.970e-105
Identity = 184/380 (48.42%), Postives = 255/380 (67.11%), Query Frame = 0

Query:  261 LAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            +A E ++ IRTV   G ++K LE Y + L++      K++    +  G A  + + +Y+   +YG  LV +       V  V  ++++G   VGQA+     +  A  AA  +FK++D KP IDS S +G + +NI+GNL F N  F YP+RKEV++L+ LNL VQ+GQT+ALVG SGCGKST +QL+QR YD   G ++++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+M+E+ +A ++AN + FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALD+ESE VVQ ALD A++GRT+I +AHRLSTI+N D I     G + E G H EL+   G+Y KL + QT
Sbjct:  284 VAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGIAFLLIYASYALAFWYGTSLVLSHEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKNGHKPDNIKGNLEFKNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENICYG--RENVTMEEIEKAVKEANAYDFIMKLPNKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDSESEAVVQVALDKARKGRTTIVVAHRLSTIRNADVIAGFDDGVIVEKGNHDELMKEEGIYFKLVTMQT 661          

BLAST of P-glycoprotein vs. nr
Match: gi|1061476161|gb|ODM94055.1| (Multidrug resistance protein 1A [Orchesella cincta])

HSP 1 Score: 582.793 bits (1501), Expect = 0.000e+0
Identity = 299/598 (50.00%), Postives = 394/598 (65.89%), Query Frame = 0

Query:  118 LDHPEEVEELPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLSE-DPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNST---------------GALCSRISSDASAI----------------------------------QGL-------------------AIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLSPNAT 646
            +D   E +   + S+ R+L  N  EW YML+G +++ + G+  PIYAI+FGEVL VLS+ D E A+++   + + FL  G++ G +MFLQ+ MFS+AGE LT R+RKM+F +ML+QE+ WFD P+N+                GA  SRI +   A+                                  QGL                   A++A+SNIRTVA L KE+ F+++Y + L++ H    K + IRG IFGF+QS+PF AY+   YYG  L+ ++ + Y +VFKV EALI GTMMVGQA AFAPNYNKA I+ +R+  LLDR+P I++    GL+I +  G +   N  FHYPTR   ++L+ ++L V+ G ++ALVGPSGCGKSTCIQL+ R+YD   GK+ ++  +I +LNV  LRS M IV+QEP LFDR++A+NIAYGDNSR VSM++++ +A+ ANIH+FIA LP GY+T VGE GTQLSGGQKQRVAIARALVRNP ILLLDEATSALDTESE+VVQ ALD A  GRTSITIAHRLS+IQNVD+I VI  G V E G+H ELL + GLY +LW+ Q  S N +
Sbjct:   21 IDELSEKQLAKQASIRRLLALNKDEWLYMLIGTISACIQGSVMPIYAIIFGEVLQVLSDPDEEKAQKDANQWALAFLGIGLLAGASMFLQLFMFSLAGEALTQRLRKMTFAAMLRQELGWFDDPANNIGALCARLSGDAASVQGATGSRIGTIVQAVSTISIAIGLAMYYKAKLGAVALAFVPLVMIATYFQGLLLFGQNFFEQKATEESSKIAVQAVSNIRTVASLCKERAFVKMYQDLLEKAHTDTLKVNLIRGFIFGFSQSIPFFAYAAVNYYGAKLITDENMPYGDVFKVGEALIFGTMMVGQALAFAPNYNKAKISGARILTLLDRRPKIENSPGVGLQIRSSNGAVKTENVAFHYPTRPSAQILKSMSLQVEPGSSVALVGPSGCGKSTCIQLILRYYDPISGKVRLDNQDIAALNVDALRSQMAIVAQEPALFDRTIAENIAYGDNSRSVSMEDIISSAKMANIHTFIAGLPAGYETRVGEMGTQLSGGQKQRVAIARALVRNPKILLLDEATSALDTESEQVVQAALDQASSGRTSITIAHRLSSIQNVDKIVVINHGVVHEVGSHEELLQKKGLYHRLWNMQGSSGNTS 618          

BLAST of P-glycoprotein vs. nr
Match: gi|1334080299|ref|XP_023564485.1| (multidrug resistance protein 1-like [Octodon degus])

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 284/540 (52.59%), Postives = 387/540 (71.67%), Query Frame = 0

Query:  103 SDRQSKRRLKRQDTILDHPEEVEE-LPKISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS--EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQGLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            S R+S      QD  L   EE+ E +P +S  RILK N +EWPY +VG+L +I+ G   P +A++F  ++GV +  +D E+ R+N   + +LFLV GIV  +  FLQ   F  AGE LT R+R + F+SML+Q+++WFD P N+TGAL +R+++DA+ ++G+A EAI N RTV  L +E+KF  +Y ++LQ P+  + K++HI G+ F F Q++ F +Y+ C  +G +LV    + ++NV  V  A++ G M VGQ ++FAP+Y KA ++AS +  + ++ P IDS ST GL+ N ++GN++F +  F YPTR ++ VL+ L+L V+ GQT+ALVG SGCGKST +QLL+RFY+   G + V+   I+ LNV  LR+ +GIVSQEP+LFD S+ +NIAYGDNSR VS +E+V+AA++ANIH FI SLP  Y+T VG+KGTQLSGGQKQR+AIARALVR P ILLLDEATSALDTESEKVVQEALD A+EGRT I IAHRLSTIQN D I VI  GRV E GTH +LLA+ G+Y  + S Q
Sbjct:  654 SVRRSMSESHSQDRKLSMKEELNESVPPLSFWRILKLNLTEWPYFVVGVLCAIINGGLQPAFAVIFSRIIGVFTRDDDEETKRQNSNLFSVLFLVLGIVSFITFFLQGFTFGKAGEILTKRLRYLVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGIATEAIENFRTVVSLTREEKFEHMYGQSLQIPYRNSLKKAHIFGITFSFTQAMMFFSYAACFRFGAFLVARGFMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIMITEKVPAIDSYSTEGLKPNMLEGNVTFHDVVFKYPTRPDIPVLQGLSLQVKKGQTLALVGSSGCGKSTTVQLLERFYNPLAGTVFVDDKEIQQLNVQWLRAQLGIVSQEPILFDCSIGENIAYGDNSRTVSQEEIVKAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPRILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADVIVVIQNGRVQERGTHQQLLAQKGIYFSMVSVQ 1193          

HSP 2 Score: 360.147 bits (923), Expect = 3.844e-106
Identity = 188/377 (49.87%), Postives = 253/377 (67.11%), Query Frame = 0

Query:  264 EAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQT 640
            E ++ IRTV   G + K LE Y + L++      K++    +  G A  + + +Y+   +YG  LV +       V  V  ++++GT  VGQA+     +  A  AA  +FK++D +P IDS S +G + +NI+GNL F N  F YP+RKEV++LR LNL VQ+GQT+ALVG SGCGKST +QLLQR YD   G + ++G +I ++NV  LR  +G+VSQEPVLF  ++A+NI YG     V+MDE+ +A ++AN ++FI  LP  +DT+VGE+G QLSGGQKQR+AIARALVRNP ILLLDEATSALDTESE VVQ ALD A+EGRT+I IAHRLST++N D I     G + E G H EL+   G+Y KL + QT
Sbjct:  246 EVLAAIRTVIAFGGQSKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTSLVLSNEYSIGQVLTVFFSVLVGTFSVGQASPNIEAFANARGAAYEIFKIIDNEPHIDSFSPNGHKPDNIKGNLEFRNIHFSYPSRKEVKILRGLNLKVQSGQTVALVGNSGCGKSTTVQLLQRLYDPSEGTVTIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG--RENVTMDEIEKAVKEANAYNFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVERGNHDELMKERGVYYKLVTMQT 620          

BLAST of P-glycoprotein vs. nr
Match: gi|321474272|gb|EFX85237.1| (ABC transporter, subfamily ABCB/MDR [Daphnia pulex])

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 304/586 (51.88%), Postives = 398/586 (67.92%), Query Frame = 0

Query:  130 ISMMRILKENSSEWPYMLVGLLASIVMGASTPIYAILFGEVLGVLS-----EDPESARENVAYYCILFLVTGIVVGLAMFLQISMFSVAGEYLTLRMRKMSFESMLKQEMAWFDRPSNSTGALCSRISSDASAIQ-------GLAIEAISNI--RTVAGLGKEQK-------FLELYL------------------EALQQPHEAAKKR-SHIRGL-----------------------------IFGF----AQSVPFLAYSGCMYYGGWLVENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQTLS 642
            +S+M I++ N  EW +++VG++ S ++G STP++AILF EVLGVL+     E+    R    +Y ++FL+ GIVVG + F Q   FS+AGE LT R+R ++F+++LKQE+ WFDR +NS GALC+R+S DA+++Q       G+  +A++ +   TV  L  + K       F+ L L                  EALQ+  + A +  S+IR +                             I GF    A S+P  AY+  MYYGGWLV N+ LD+ +VFKV+E+L+ GT M+GQA AFAPNYNKA +AA+R+F LL R P ID+ S +GL + N+ GN++F   RF YPTRK+  VL+ L+L+V+AGQT+ALVG SGCGKSTCIQLL+RFYD   G++ ++G +I  +N+  LRS MGIVSQEP+LF+ ++A NIAYGDNSR V MDE++EAARKANIH FI SLP GY+TMVGE+GTQLSGGQKQRVAIARAL+RNP ILLLDEATSALD+ESE VVQ ALD A+EGRT ITIAHRLSTIQN D I VI  G +SE GTH EL+  GGLY +L S Q ++
Sbjct:  740 VSLMDIMRMNRKEWHFIVVGVIGSAIVGLSTPVFAILFSEVLGVLTPGGSAEEQAEKRAQGNFYALMFLILGIVVGFSAFAQSFSFSIAGESLTSRLRGLTFQAILKQEIGWFDRKTNSVGALCARLSGDAASVQGATGSRIGVLFQAVTTMIASTVLALYFQWKLGLVALCFVPLLLVSTYFQAKIIMGQSALEREALQKSAKVAMEAISNIRTVASLGKERQFHTIYMESLRGPHKEALKKSWIRGFIFGFASSIPMFAYAVTMYYGGWLVVNECLDFTSVFKVSESLLFGTQMIGQAVAFAPNYNKAKVAANRIFALLRRVPQIDASSNNGLVLENVDGNVNFEQVRFRYPTRKDAEVLQGLSLAVRAGQTVALVGHSGCGKSTCIQLLERFYDPDSGQVQLDGQDINPVNISSLRSQMGIVSQEPILFNLTIAQNIAYGDNSRVVPMDEIIEAARKANIHVFIQSLPNGYETMVGERGTQLSGGQKQRVAIARALIRNPKILLLDEATSALDSESEHVVQMALDAAREGRTCITIAHRLSTIQNADNIIVINHGTISEQGTHEELIKLGGLYFELCSVQGIA 1325          

HSP 2 Score: 350.132 bits (897), Expect = 9.343e-102
Identity = 180/384 (46.88%), Postives = 255/384 (66.41%), Query Frame = 0

Query:  260 GLAIEAISNIRTVAGLGKEQKFLELYLEALQQPHEAAKKRSHIRGLIFGFAQSVPFLAYSGCMYYGGWLV----ENQGLDYKNVFKVAEALILGTMMVGQATAFAPNYNKALIAASRVFKLLDRKPLIDSESTSGLRINNIQGNLSFSNARFHYPTRKEVRVLRDLNLSVQAGQTIALVGPSGCGKSTCIQLLQRFYDLQGGKLNVEGSNIESLNVPMLRSTMGIVSQEPVLFDRSLADNIAYGDNSRQVSMDEVVEAARKANIHSFIASLPRGYDTMVGEKGTQLSGGQKQRVAIARALVRNPAILLLDEATSALDTESEKVVQEALDNAQEGRTSITIAHRLSTIQNVDRIFVIAKGRVSESGTHGELLARGGLYSKLWSTQ 639
            G+A E  S+IRTV   G ++K ++ + + L    +A  KR    G+  G    + + +Y+   +YG  L+    +       ++  V  ++++G M +GQA  +   ++ A  AA+ +F ++DR P IDS S  GL  + + G +SF +  F+YP+R +V++L+ ++  V  GQT+ALVG SGCGKSTCIQLLQRFYD   G + ++G+ + +LN+  LR  MG+V QEPVLF  S+ +NI YG +   VS +E+  AA++AN H FI  LPR YDT+VGE+G QLSGGQKQR+AIARALVR P ILLLDEATSALDT+SE VVQ+ALD A++GRT+I +AHRL+TI+N DRI V+  G V E GTH +L+A  G+Y +L   Q
Sbjct:  306 GIAEEVFSSIRTVMAFGGQRKEIDRFQDDLAYAKKAGIKRGMATGIGAGLVWGIIYASYALAFWYGITLILAACDGNSYSSSDLLIVFFSVLIGAMQIGQAAPYMEAFSVARGAAATIFAIIDRVPPIDSSSNEGLVPDGVDGKISFRDVFFNYPSRPDVKILQGISFDVTPGQTVALVGTSGCGKSTCIQLLQRFYDPLEGSVTIDGNELRNLNLGWLRDQMGMVGQEPVLFGTSIGENICYGRDG--VSKEEMERAAKEANAHDFIQRLPRKYDTLVGERGGQLSGGQKQRIAIARALVRQPKILLLDEATSALDTQSEAVVQKALDKARQGRTTIIVAHRLTTIRNADRIIVMKDGIVQEDGTHDKLMALNGIYYQLVIAQ 687