BLAST of aconitate hydratase 1 vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 830.861 bits (2145), Expect = 0.000e+0
Identity = 424/869 (48.79%), Postives = 575/869 (66.17%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDAD---YAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAET-----FRRPMGKE---VTVVGED---YTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYT-KGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
            + KLP +++++LE+ +R  D  F V   D++    W    GKN  EI +RP RV++QDFTGVPA+VD AAMRD +  LGGD  +INP  P DLVIDHS+ +D     +A   N D E++RN ER+TFLKWG  +F N  ++PPG+GI HQVNLEYL++  +TDTD     + +PD++VGTDSHTTM+NGL V+GWGVGGIEAEA MLGQP+SM++PEVVG+ ++G        TD+VL + + LR  GVVG+FVEFYG G+  L +ADRATI+NM PEYGAT G FP+D  ++ YLR T R+  ++  ++ Y       RD +     P ++  + LD+GT+V + SGPKRPQD VA+T+ K  F + ++       F  P  K+   V ++  D   Y +  G V+IA+ITSCTNTSNP VM+GAGL+A+KA   GL   P++KTSL+PGS VV+ YL+ +G+   LD +GF++VGYG  TCIGNSGP++  +     +GDLV   VLSGNRNFEGRI+P  RANYLASPPLV+AYA+AG +DID   EALG D N   ++L+DIWPT  EI  + +K +    F   Y+ V  G+E+W +++ P    Y W   STYI++PP+F  MS++  +   +  A  L  LG   T DHISPAG    + PA +YL  + +  REFNSYGSRRGN  +M RGTFANIR+ N+ L G  G  T     G    +FDAAM ++ E   L++ AG+ YG GSSRDWAAKG  LLGV+AVIAE++ERIHRSNLVGMG++P +++ G+S +SLG +G E  ++     + P Q +   +   DGS K      R DT +E+ Y  +GG+L+Y++R +
Sbjct:   31 YHKLPYSIRILLESAVRNCDN-FQVYEKDVETILNWEGNRGKN-AEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTD-----LVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKV 892          

BLAST of aconitate hydratase 1 vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 375/898 (41.76%), Postives = 535/898 (59.58%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMID------EFGNP-------------------------RAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAP--IYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFT-----REMAETFRRPMGK-----EVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGP-IQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALG-QDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDS-ETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYT-KGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVL 872
            + +LP +L+V+LE  +R                SE   +  K+  E   R  RVL+QDFTGVPA++DLAA+R+ +   GG    ++ L P DLV+DH V +D      +  +P                          A + N   E+ERN ER+ FLKW   AF N  V+PPGTG+ HQVNLEYL++ V   T Q GV   +PD++VGTD+HTTM+NGL VLGWGVG ++A+A M G PV++  P VVG  + G + + +T TDLVL + + LR  GV G FVEF+G  L  L +ADRATI+NM PEYGA  GFFPID  T++YL  +GRD+  I  VE Y K    +R +D      + + + +D+  I+P +SGPKR +D V++      F      +E  + +    G+      V + GE +T+  G V++A+I+SCTNTSNP VM+GAGL+A+KA   GL+    VKTSLAPGS VV+ YL+ +G+   L  +GF +VGYGC+TC  NSGP +   +  AI QG+LV   +LSGNRNFEGR+ PD+RANYLASP +V+AYA+AG +DI+L  E LG     G  +FLK+IWP+  EI  +  K V    + + Y+ V  G++ W ++  P+S + + W + STYI+ P Y + +  +     N+   + L  LGD +T+DHISPAGS   S+PA +YL    +  RE+NSYG+RRGN+ VM+RGTF+N+R++N +L+ V G YT   P      IF+A+  ++ +G  L    G+ +G G+SRDWA +G  LLGV+A++A +F+  +RSNLV  G++P + T   S     L G E ++I   D I P   +   +  A+ S K +    R+D   E +    GGV+  ++
Sbjct:   37 YEELPYSLRVLLECAVRKR--------------SETQGQVWKDVYERILR--RVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVV---TCQGGV--LFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYIKEIGSNARNWKNM---RCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLLNKV-GPYTLHLPSEVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQIT---SISYESLKGTECLSIDIRDNITPKCLIDVVVGKAERSFKAVV---RVDNDYETDVFHAGGVIAQIV 903          

BLAST of aconitate hydratase 1 vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 132.494 bits (332), Expect = 1.190e-31
Identity = 141/493 (28.60%), Postives = 216/493 (43.81%), Query Frame = 0

Query:   71 RPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTL-VGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIAS--ITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRAN-YLASPPLVVAYALAGTMDIDLATEAL 559
            RP RV MQD T   A++   +     VA+           P  +  DH +     G     + ++ R  + N E Y FL      +       PG+GI HQ+ LE                 A+P  L VGTDSHT    GL  L  GVGG +A   M   P  +  P+V+G +LTG++   T+  D++LKV  +L   G  G  VE++G G+D +      TI NM  E GAT   FP +     YL +T R  D   L + Y +        D    Y   + +D+ T+ P ++GP               FT ++A     P+G+    +GE        + +    I SCTN+S   +     ++ ++A A G+  K      + PGS+ +   +E  GL + + + G  ++   C  CIG            I +G+   T V S NRNF GR   +   + ++ SP + VA +LAG++D D  ++A+
Sbjct:  675 RPDRVAMQDATAQMAMLQFISSGLPKVAV-----------PSTIHCDHLIQAQIEG-----KKDLARAKDLNAEVYNFLSTSGAKYG-VGFWKPGSGIIHQIILE---------------NYAFPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNR-SDIANLADKYRE---VLLSPDGGCKYDQVVEIDLNTLEPHVNGP---------------FTPDLAT----PIGE----LGEKAKKNGWPLEVKDGLIGSCTNSSYEDMARXTAII-KQALAKGMKSKS--NFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWD------RQNITKGE-KNTIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAI 1098          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|30173505|sp|P70920.2|ACNA_BRADU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1119.76 bits (2895), Expect = 0.000e+0
Identity = 553/880 (62.84%), Postives = 674/880 (76.59%), Query Frame = 0

Query:   14 AEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGV-------EIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYA-PIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPM--GKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGV-----EGGYTKG-PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGL-DTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLS 876
            AE  GL   SKLP ++KV+LEN+LR EDG  SV   DI A S+W  K      EIA+RPARVLMQDFTGVPAVVDLAAMR+ +  LGGDA++INPL PVDLVIDHSV+++ FG+ +AF  NV  EY++N ERY FLKWGQ AF+NF VVPPGTGICHQVNLEYLSQTVWT  ++  V       E+AYPD+LVGTDSHTTMVNGLAVLGWGVGGIEAEA MLGQP+SML+P VVGF+L G M EG T TDLVL V +MLR  GVVGKFVEF+G GLD L +AD+ATIANMAPEYGATCGFFP+D   + YL+ +GR   R+ALV+AYAK  G +R A  A P++T TL+LD+  +VP+++GPKRP+  +AL +    F+  +A  +++     K   V G+ Y +  G VVIA+ITSCTNTSNP V+IGAGL+AR AAA GL  KPWVKTSLAPGSQVV+ YL  +GLQ  LDK+GFNLVG+GCTTCIGNSGP+ +E+S +I    +VA +VLSGNRNFEGR+SPDV+ANYLASPPLVVA+ALAG++  +LA E LG+ ++G  V+LKDIWPTS EINA ++K VT   F KKYADVFKGD  W+ ++T +SETY W  +STY+QNPPYF+GM K+P  ++++  A++LA+ GD +TTDHISPAGS K ++PAG+YL E QV   +FN YG+RRGNHEVMMRGTFANIRIKN ML G      EGG TK  PDG Q +I+DAAM +Q E  PLV+FAG +YG GSSRDWAAKGT LLGV+AVI ++FERIHRSNLVGMGV+P  F  G S  SLGL GDE V + GL   ++P Q +TA I   DGS + +++ CRIDT  E++Y  NGG+LHYVLR L+
Sbjct:   28 AEKNGLKGISKLPYSMKVLLENLLRNEDGR-SVKKADIVAVSKWLRKKSLE-HEIAFRPARVLMQDFTGVPAVVDLAAMRNAMQKLGGDAEKINPLVPVDLVIDHSVIVNFFGDNKAFAKNVTEEYKQNQERYEFLKWGQAAFSNFSVVPPGTGICHQVNLEYLSQTVWTKKEKMTVGKKTGTFEVAYPDSLVGTDSHTTMVNGLAVLGWGVGGIEAEACMLGQPLSMLLPNVVGFKLKGAMKEGVTATDLVLTVTQMLRKLGVVGKFVEFFGPGLDHLSVADKATIANMAPEYGATCGFFPVDAAAIDYLKTSGRAAPRVALVQAYAKAQGLFRTAKSADPVFTETLTLDLADVVPSMAGPKRPEGRIALPSVAEGFSVALANEYKKTEEPAKRFAVEGKKYEIGHGDVVIAAITSCTNTSNPSVLIGAGLLARNAAAKGLKAKPWVKTSLAPGSQVVAAYLADSGLQAHLDKVGFNLVGFGCTTCIGNSGPLPEEISKSINDNGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAHALAGSVTKNLAVEPLGEGKDGKPVYLKDIWPTSKEINAFMKKFVTASIFKKKYADVFKGDTNWRKIKTVESETYRWNMSSTYVQNPPYFEGMKKEPEPVTDIVEARILAMFGDKITTDHISPAGSIKLTSPAGKYLSEHQVRPADFNQYGTRRGNHEVMMRGTFANIRIKNFMLKGADGNIPEGGLTKHWPDGEQMSIYDAAMKYQQEQVPLVVFAGAEYGNGSSRDWAAKGTRLLGVRAVICQSFERIHRSNLVGMGVLPLTFEEGTSWSSLGLKGDEKVTLRGLVGDLKPRQKLTAEIVSGDGSLQRVSLLCRIDTLDELDYYRNGGILHYVLRKLA 905          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81522761|sp|Q8ZP52.1|ACNA_SALTY (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=2-methyl-cis-aconitate hydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=RNA-binding protein)

HSP 1 Score: 1080.09 bits (2792), Expect = 0.000e+0
Identity = 536/870 (61.61%), Postives = 671/870 (77.13%), Query Frame = 0

Query:   13 PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTRE------MAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQA-AIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
            P  A  LGD ++LP +LKV+LEN+LR++DG  SV+ +DI+A + W  K     REIA+RPARVLMQDFTGVPAVVDLAAMR+ +  LGGD  ++NPL+PVDLVIDHSV +D FG+  AF+ NV  E ERN ERY FLKWG++AF+ F VVPPGTGICHQVNLEYL + VW++  Q+G  IAYPD+LVGTDSHTTM+NGL VLGWGVGGIEAEAAMLGQPVSMLIP+VVGF+LTG++ EG T TDLVL V +MLR HGVVGKFVEFYG GLD LPLADRATIANM+PEYGATCGFFPID  TL Y+R +GR +D + LVE YAK  G WR+    P++T+TL LDMG +  +++GPKRPQD VAL +   AF          A+  R+P+  + T+ G+ Y +  G VVIA+ITSCTNTSNP V++ AGL+A+KA  LGL R+PWVK SLAPGS+VVS+YL  A L   LD++GFNLVGYGCTTCIGNSGP+ + +  AI +GDL   +VLSGNRNFEGRI P V+ N+LASPPLVVAYALAG M+I+LAT+ LG D+ G+ V+LKDIWP++ EI   VE  V+ + F K+YA+VF+G E+W+++Q   S+TY W + STYI+  P+F  M   P  + ++ GA++LA+LGD VTTDHISPAGS K  +PAG+YL    V  ++FNSYGSRRGNHEVMMRGTFANIRI+NEML GVEGG T+   G +A +I+DAAM +Q E TPL + AG++YG+GSSRDWAAKG  LLG++ VIAE+FERIHRSNL+GMG++P EF  G +RK+LGLTG+E + IA L  + P   +  T+T +DGS + +  +CRIDTA E+ Y +N G+LHYV+RN+
Sbjct:   26 PLAAKSLGDIARLPKSLKVLLENLLRWQDGE-SVTDEDIQALAGWL-KNAHADREIAWRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTSKVNPLSPVDLVIDHSVTVDHFGDDDAFEENVRLEMERNHERYMFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSEL-QDGEWIAYPDSLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAITLEYMRLSGRSDDLVELVETYAKAQGMWRNPGDEPVFTSTLELDMGDVEASLAGPKRPQDRVALGDVPKAFAASAELELNTAQRDRQPV--DYTMNGQPYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAQAKLTPYLDELGFNLVGYGCTTCIGNSGPLPEPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLATDPLGYDRKGDPVYLKDIWPSAQEIARAVE-LVSSDMFRKEYAEVFEGTEEWKSIQVESSDTYGWQSDSTYIRLSPFFDEMQAQPAPVKDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQNHGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMLPGVEGGMTRHLPGTEAMSIYDAAMLYQQEKTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEVIDIADLQNLRPGATIPVTLTRSDGSKETVPCRCRIDTATELTYYQNDGILHYVIRNM 889          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|166215073|sp|P25516.3|ACNA_ECOLI (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=RNA-binding protein; AltName: Full=Stationary phase enzyme)

HSP 1 Score: 1074.69 bits (2778), Expect = 0.000e+0
Identity = 535/870 (61.49%), Postives = 662/870 (76.09%), Query Frame = 0

Query:   13 PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTR----EMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG-PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSK 877
            P  A  LGD ++LP +LKV+LEN+LR++DG  SV+ +DI A + W  K     REIAYRPARVLMQDFTGVPAVVDLAAMR+ +  LGGD  ++NPL+PVDLVIDHSV +D FG+  AF+ NV  E ERN ERY FLKWG++AF+ F VVPPGTGICHQVNLEYL + VW++  Q+G  IAYPDTLVGTDSHTTM+NGL VLGWGVGGIEAEAAMLGQPVSMLIP+VVGF+LTG++ EG T TDLVL V +MLR HGVVGKFVEFYG GLD LPLADRATIANM+PEYGATCGFFPID  TL Y+R +GR ED++ LVE YAK  G WR+    PI+T+TL LDM  +  +++GPKRPQD VAL +   AF      E+  T +     +  + G  Y +  G VVIA+ITSCTNTSNP V++ AGL+A+KA  LGL R+PWVK SLAPGS+VVS+YL  A L   LD++GFNLVGYGCTTCIGNSGP+   +  AI + DL   +VLSGNRNFEGRI P V+ N+LASPPLVVAYALAG M+I+LA+E +G D+ G+ V+LKDIWP++ EI   VE+ V+ E F K+YA+VF+G  +W+ +    S+TY W   STYI+  P+F  M   P  + ++ GA++LA+LGD VTTDHISPAGS K  +PAG+YL  R V  ++FNSYGSRRGNHEVMMRGTFANIRI+NEM+ GVEGG T+  PD    +I+DAAM ++ E TPL + AG++YG+GSSRDWAAKG  LLG++ VIAE+FERIHRSNL+GMG++P EF  G +RK+LGLTG+E + I  L  ++P   V  T+T ADGS + +  +CRIDTA E+ Y +N G+LHYV+RN+ K
Sbjct:   26 PLAAKSLGDITRLPKSLKVLLENLLRWQDGN-SVTEEDIHALAGWL-KNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSEL-QDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQ-VSTEMFRKEYAEVFEGTAEWKGINVTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK 891          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81622450|sp|Q9I3F5.1|ACNA_PSEAE (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1073.54 bits (2775), Expect = 0.000e+0
Identity = 550/888 (61.94%), Postives = 672/888 (75.68%), Query Frame = 0

Query:   13 PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVV-----------------------GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYT-KGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGL-DTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
            P  A  LGD  KLP +LKV+LEN+LR+EDG  +V+ DD+KA + W  +  ++ REI YRPARVLMQDFTGVPAVVDLAAMR  +   GGD Q+INPL+PVDLVIDHSVM+D+F +  AF+ NV+ E +RN ERY FL+WGQ AF+NF VVPPGTGICHQVNLEYL +TVWT  D++G   A+PDTLVGTDSHTTM+NGL VLGWGVGGIEAEAAMLGQPVSMLIPEV+GF+LTG++ EG T TDLVL V +MLR  GVVGKFVEFYG GL  LPLADRATIANMAPEYGATCGFFP+D  TL YLR +GR E  + LVEAY+KE G WR+  + P++T TL LDMG +  +++GPKRPQD VAL N  +AF   +         +E  ++                       G+ + +++G VVIA+ITSCTNTSNP VM+ AGL+A+KA   GL RKPWVK+SLAPGS+VV++Y +AAGL   LD++GF+LVGYGCTTCIGNSGP+ + +  AI Q DL   SVLSGNRNFEGR+ P V+ N+LASPPLVVAYALAG++ I+L+ E LG  ++G  V+LKDIWP+  EI   ++K V  E F K+YA+VF GDEKWQA+Q P S+TY+W A STYIQ+PP+F+ +++ P  I+++  A+VLAVLGD VTTDHISPAG+ K  +PAG+YL E  V  ++FNSYGSRRGNHEVMMRGTFANIRIKNEML G EGG T   P G + AI+DAAM +Q +GTPLVI AG++YG GSSRDWAAKGT LLGVKAVIAE+FERIHRSNLVGMGV+P +F  G  RKSL LTG E + I GL   ++P   ++  +T  DGS     V CRIDT  E+EY + GG+LHYVLR++
Sbjct:   26 PEAARTLGDLGKLPMSLKVLLENLLRWEDGS-TVTGDDLKALAGWL-RERRSDREIQYRPARVLMQDFTGVPAVVDLAAMRAAMAKAGGDPQKINPLSPVDLVIDHSVMVDKFASESAFEQNVEIEMQRNGERYAFLRWGQNAFDNFSVVPPGTGICHQVNLEYLGRTVWT-KDEDGRTYAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGKLREGITATDLVLTVTQMLRKKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGFFPVDEITLGYLRLSGRPESTVKLVEAYSKEQGLWREKGHEPVFTDTLHLDMGEVEASLAGPKRPQDRVALQNVASAFNEFLGLQLHPSSTEEGRLLSEGGGGTAVGANAAFGEIDYQHDGQTHRLKNGAVVIAAITSCTNTSNPSVMMAAGLLAKKAVEKGLQRKPWVKSSLAPGSKVVTDYFKAAGLTRYLDELGFDLVGYGCTTCIGNSGPLLEPIEKAIQQADLTVASVLSGNRNFEGRVHPLVKTNWLASPPLVVAYALAGSVRINLSEEPLGTGKDGQPVYLKDIWPSQKEIAEAIQK-VDTEMFHKEYAEVFAGDEKWQAIQVPQSDTYEWQADSTYIQHPPFFEHIAEAPPAIADVEQARVLAVLGDSVTTDHISPAGNIKADSPAGRYLREHGVEPKDFNSYGSRRGNHEVMMRGTFANIRIKNEMLGGEEGGNTLYVPSGEKLAIYDAAMRYQEDGTPLVIVAGKEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFENGQDRKSLKLTGKEVLNIRGLGGELKPHMPLSVEVTREDGSQDSFKVLCRIDTLNEVEYFKAGGILHYVLRSM 909          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|543763|sp|P37032.1|ACON_LEGPH (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=IP210; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Major iron-containing protein; Short=MICP; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1069.69 bits (2765), Expect = 0.000e+0
Identity = 538/866 (62.12%), Postives = 661/866 (76.33%), Query Frame = 0

Query:   14 AEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDAD-YAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAET-FRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG-PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLS 876
            AE       ++LP +LKV+LEN+LRFEDG  +V+  DIKA ++W      +  EIA+RP RVLMQDFTGVPAVVDLAAMR  IV +GG+A +I+PL+PVDLVIDHSVM+D+F +  A ++N   E ERN ERY FL+WGQKAF+NF+VVPPGTGICHQVNLEYL +TVW +++ +G   AYPDTLVGTDSHTTM+NGL VLGWGVGGIEAEAAMLGQPVSMLIPEV+GF+L+G++ EG T TDLVL V +MLR  GVVGKFVEFYG GL+ LPLADRATI+NMAPEYGATCGFFP+D ET++YL  TGRD+  IALVEAYAK  G W D D   P++T +L LD+G++ P+++GPKRPQD V L++    F   + E    +   K   V  +D+ M+ G VVIA+ITSCTNTSNP V++ AGLVA+KA   GL RKPWVK+SLAPGS+VV++YL  AGLQ  LD++GFNLVGYGCTTCIGNSGP+  ++S  +A+ DLV +SVLSGNRNFEGR+ P VRAN+LASPPLVVAYAL GT   DL+ E +GQD+ GNDV+LKDIWP++ EI A V K V+   F K+YA+VFKGD  WQA+QT   +TY+W   STYIQ+PP+F+ +S  P  +  +  A VLA+ GD +TTDHISPAGS K S+PAG YL  + V  ++FNSYGSRRGNHEVMMRGTFANIRI+NEM  G EGG T+  P G   +I+DAAM +Q     LVI AG++YG GSSRDWAAKGT LLGVKAVI E+FERIHRSNL+GMG++P +F  G +RK+L L G E ++I   D + P   V  TI   DG  ++I   CRIDTA E+EY +NGG+L YVLR +S
Sbjct:   29 AENKHFKGINRLPYSLKVLLENLLRFEDGN-TVTTKDIKAIADWL-HNKTSQHEIAFRPTRVLMQDFTGVPAVVDLAAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNKERYEFLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVW-NSENDGQLYAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQMLRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGRDKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSLPVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLVAKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNSGPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCSDLSREPIGQDKEGNDVYLKDIWPSNEEIAAEVAK-VSGTMFRKEYAEVFKGDAHWQAIQTSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGSIKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTRYVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESFERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAMVPVTIERQDGDIEKIETLCRIDTADELEYYKNGGILQYVLRKIS 890          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|88909586|sp|Q4JVM4.1|ACNA_CORJK (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1023.08 bits (2644), Expect = 0.000e+0
Identity = 513/911 (56.31%), Postives = 650/911 (71.35%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREM--------------------------AETFRRPM--------------GKE--------VTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG---PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQN------VTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
              KLP +LKV+ EN+LR EDG  +++ + I+A + W D   +   EI + PARV+MQDFTGV  +VDLA +RD +VALGGDA  +NPLNP ++VIDHSV+I+ FG+  A + NV+ EY+RN ERY FL+WG  AF NFRVVPPGTGI HQVN+EYL+++V+   D NG  +AYPDT VGTDSHTTM NGL +LGWGVGGIEAEAAMLGQP+SMLIP VVGF+LTG++  G T TD+VL + +MLR HGVVGKFVEFYG G+  LPLA+RATI NM+PE+G+T   FPID ET++YL  TGRD++ +  VEAYAK  G W D +    Y+  L LD+ T+VP+I+GPKRPQD + L ++K  F +++                          A+T   P               G E        V   GED  ++ G V IASITSCTNTSNP VM+GAGL+AR AAA GL   PWVKTS+APGSQVV+ Y E AGL +DL+ +GF LVGYGCTTCIGNSGP+ +E+S  I +GDL AT+VLSGNRNFEGRI+PDV+ NYLASP LV+AYA+AGTMD D  T+ LGQDQ+GNDVFLKDIWP++ +I  ++  ++T++ + + YA+VF+GDE+W+++  P  +T+DW   STYI+  PYF GMSK+P  ++++ GA+VLA+LGD VTTDHISPA + K  TPA QYL    V  +++NS G+RRGNHEVM+RGTFANIR++N++LDGV GGYT+      G Q+ I+DAAM +Q E TPLV+  G++YG GSSRDWAAKGT LLGVKAVIAE+FERIHRSNL+GMGV+P +F  G+S KSLG+ G ET  I G   IE L N      V  T T  +G   E     RIDT  E +Y  NGG+L +VLRN+
Sbjct:   32 MEKLPYSLKVLGENLLRNEDGK-NITREHIEAIANW-DPSAEPNFEIQFTPARVIMQDFTGVACIVDLATIRDAVVALGGDADDVNPLNPAEMVIDHSVIIEAFGDSDALEKNVEIEYQRNDERYKFLRWGTGAFENFRVVPPGTGIVHQVNIEYLARSVF---DNNG--LAYPDTCVGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPISMLIPRVVGFKLTGEIPTGVTATDVVLTITDMLRQHGVVGKFVEFYGKGVGELPLANRATIGNMSPEFGSTAAMFPIDEETVKYLELTGRDQETLERVEAYAKAQGMWLDPEKEVEYSEYLELDLSTVVPSIAGPKRPQDRIELNDSKAQFRKDLHNYVEADASAVTPDFDAEGPATENTSAQTAGTPASAADAKGNIPSAAAGAEGRPSNPVTVNYNGEDIELDHGMVAIASITSCTNTSNPSVMVGAGLLARNAAAKGLKSAPWVKTSMAPGSQVVNGYYEKAGLWKDLEAMGFYLVGYGCTTCIGNSGPLPEEISAGINEGDLAATAVLSGNRNFEGRINPDVKMNYLASPILVIAYAIAGTMDFDFETQPLGQDQDGNDVFLKDIWPSTEDIEEVIASSITKDLYAEDYANVFEGDERWRSLDVPSGKTFDWDPKSTYIRKAPYFDGMSKEPEAVNDVKGARVLALLGDSVTTDHISPASTIKPGTPAAQYLDANGVERKDYNSLGARRGNHEVMVRGTFANIRLQNQLLDGVSGGYTRDFTQEGGPQSFIYDAAMNYQKENTPLVVLGGKEYGTGSSRDWAAKGTLLLGVKAVIAESFERIHRSNLIGMGVVPLQFPEGESWKSLGIEGTETFDIEG---IEELNNGSTPKTVKVTATKENGEKIEFDAVTRIDTPGEADYYRNGGILQFVLRNM 932          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|122426093|sp|Q1RKD5.1|ACNA_RICBR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1012.68 bits (2617), Expect = 0.000e+0
Identity = 491/859 (57.16%), Postives = 630/859 (73.34%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRR---PMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGP-DGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGL-DTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
              KLP +L+V+ EN+LR      + S  ++  F EW  K  K+  EI + PARVLMQDFTGVPA+VDLAAMRD +  +GGD  +INPL PVDLVIDHSV +D + +  +F  NV  E  RN+ERY FLKWGQ+AFNNF+VVPPGTGICHQVNLEYL++ VW     NGV  AYPD+LVGTDSHTTMVNGL+VLGWGVGGIEAEAAMLGQP++M++PEV+G +LTG++    T TDLVLK+ EMLR   VVGKFVEFYG GL  + +ADRATI+NMAPEYGATCGFFPID ET++YL  TGRD+++I LVE YAK    W + D A  YT  L LD+  +  +++GP+RPQD V L +  + F +E++ TF      +  +  V  ++Y + +G VVIA+ITSCTNTSNP VMIGA L+A+KA   GL  KPWVKTSLAPGS+VV+EYL+++GL + LD++GFNLVGYGCTTCIGNSGP+  E+ + I +  LV  SVLSGNRNFEGRI+P  +A+YLASP LVVAYAL+G+++IDL    LG++  G DV+LKDIWP+  EI+ ++  ++    F++KY+D+F G ++WQ+++   S  Y W  +STYI NPPYF+ +      I ++  A++LA+ GD +TTDHISPAGS   ++PA +YL + Q+   +FNSYGSRRGNHEVMMRGTFANIRIKNEM  GVEGG+T       Q  I+DAAM ++A G   VIFAG++YG+GSSRDWAAKG  LLGVKAVIAE+FERIHRSNLVGMGV+P  FT   +R  L L G E++ I GL + I+P  +V   I   +G  + I +  +I T  EI YI++G ++H+V+ NL
Sbjct:   36 LKKLPYSLRVLFENVLR------TGSKQNLMVFKEWL-KNKKSDAEIDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYASGSSFDKNVAMEMRRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVW---HSNGV--AYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGTATATDLVLKITEMLRKKKVVGKFVEFYGEGLKAMTIADRATISNMAPEYGATCGFFPIDQETIKYLELTGRDKEQIKLVEEYAKAQDLWCNFDDAAEYTDILELDLSEVTSSLAGPRRPQDRVNLGDVSSGFKKELS-TFSSNNISIDTKHAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKAIEQGLKVKPWVKTSLAPGSKVVTEYLKSSGLNQYLDQLGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRINPLTKASYLASPILVVAYALSGSLNIDLTNHPLGKNDKGRDVYLKDIWPSKEEIDKVIANSINSSMFVEKYSDIFSGTKEWQSLEVTSSSNYAWDKSSTYINNPPYFENIG-SKNSIKDIKSARILAIFGDSITTDHISPAGSISKTSPAAKYLTDHQISPIDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLKNMQQTIYDAAMDYKANGVSAVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGVLPLIFTNNMTRFDLKLDGSESIDIIGLNEHIKPYNSVKCIIKKQNGEMQTIDLILQIFTDNEINYIKHGSIMHFVVENL 880          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|88909585|sp|Q8NQ98.2|ACNA_CORGL (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1012.29 bits (2616), Expect = 0.000e+0
Identity = 514/915 (56.17%), Postives = 645/915 (70.49%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPI-YTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAF--------------------TREMAETFRRPMG---------------------------KEVTVV---GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG--PDGA-QAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTI---EPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
              KLP +LKV+ EN+LR EDG  +++ + I+A + W D       EI + PARVLMQDFTGVP VVDLA MR+ + ALGGD   +NPLNP ++VIDHSV+++ FG P A   NV+ EYERN ERY FL+WG ++F+NFRVVPPGTGI HQVN+EYL++ V+   D  G  +AYPDT +GTDSHTTM NGL +LGWGVGGIEAEAAMLGQPVSMLIP VVGF+LTG++  G T TD+VL + EMLR HGVV KFVEFYG+G+  +PLA+RATI NM+PE+G+TC  FPID ET +YLR TGR E+++ALVEAYAK  G W D D     Y+  L LD+ T+VP+I+GPKRPQD + L+ AK  F                    TR + E   +P G                           K VTV    G +YT++ G V IASITSCTNTSNP VMIGAGL+ARKAA  GL  KPWVKT  APGSQVV  Y + A L +DL+ +GF L G+GCTTCIGNSGP+ +E+S AI + DL AT+VLSGNRNFEGRISPDV+ NYLASP +V+AYA+AGTMD D   EALGQDQ+GNDVFLKDIWP++ EI   +++ ++RE +   YADVFKGD++WQ +  P  +T++W   STYI+  PYF GM  +P  ++++ GA+VLA LGD VTTDHISPA S K  TPA QYL E  V   ++NS GSRRGNHEVMMRGTFANIR++N+++D + GGYT+    +GA QA I+DA++ ++A G PLV+  G++YG GSSRDWAAKGT LLG++AVI E+FERIHRSNL+GMGV+P +F  G+S +SLGL G ET  I GL  +   E  + V  T T  +G   E     RIDT  E +Y  +GG+L YVLR ++ ++
Sbjct:   32 MEKLPYSLKVLGENLLRTEDGA-NITNEHIEAIANW-DASSDPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVAALGGDPNDVNPLNPAEMVIDHSVIVEAFGRPDALAKNVEIEYERNEERYQFLRWGSESFSNFRVVPPGTGIVHQVNIEYLARVVF---DNEG--LAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPVGVTATDVVLTITEMLRDHGVVQKFVEFYGSGVKAVPLANRATIGNMSPEFGSTCAMFPIDEETTKYLRLTGRPEEQVALVEAYAKAQGMWLDEDTVEAEYSEYLELDLSTVVPSIAGPKRPQDRILLSEAKEQFRKDLPTYTDDAVSVDTSIPATRMVNEGGGQPEGGVEADNYNASWAGSGESLATGAEGRPSKPVTVASPQGGEYTIDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAEKGLKSKPWVKTICAPGSQVVDGYYQRADLWKDLEAMGFYLSGFGCTTCIGNSGPLPEEISAAINEHDLTATAVLSGNRNFEGRISPDVKMNYLASPIMVIAYAIAGTMDFDFENEALGQDQDGNDVFLKDIWPSTEEIEDTIQQAISRELYEADYADVFKGDKQWQELDVPTGDTFEWDENSTYIRKAPYFDGMPVEPVAVTDIQGARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDEHGVERHDYNSLGSRRGNHEVMMRGTFANIRLQNQLVD-IAGGYTRDFTQEGAPQAFIYDASVNYKAAGIPLVVLGGKEYGTGSSRDWAAKGTNLLGIRAVITESFERIHRSNLIGMGVVPLQFPAGESHESLGLDGTETFDITGLTALNEGETPKTVKVTATKENGDVVEFDAVVRIDTPGEADYYRHGGILQYVLRQMAASS 938          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|88909584|sp|Q8FTA8.2|ACNA_COREF (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1008.82 bits (2607), Expect = 0.000e+0
Identity = 511/915 (55.85%), Postives = 642/915 (70.16%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPI--YTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRP-----------MGKE------------------------------------VTVV---GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG---PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEP---LQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
              KLP +LKV+ EN+LR EDG  +++ + I+A + W D       EI + PARVLMQDFTGVP VVDLA MR+ +  LGGD  ++NPLNP ++VIDHSV+++ FG P A + NV+ EYERN ERY FL+WG +AF+NFRVVPPGTGI HQVN+EYL++ V+   D +G  +AYPDT +GTDSHTTM NGL +LGWGVGGIEAEAAMLGQPVSMLIP VVGF+LTG++  G T TD+VL + EMLR HGVV KFVEFYG G+  +PLA+RATI NM+PE+G+TC  FPID ET++YLR TGR E++IALVEAYAK  G W + D AP   Y+  L LD+ T+VP+I+GPKRPQD + L+ AK  F  ++      P           M  E                                    VTVV   G +YT++ G V IASITSCTNTSNP VMIGAGL+ARKAA  GL  KPWVKT  APGSQVV  Y + A L +DL+ +GF L G+GCTTCIGNSGP+ +E+S+AI + DL AT+VLSGNRNFEGRISPDV+ NYLASP +V+AYA+AGTMD D   EALGQDQ+GNDVFLKDIWP++ EI   ++  ++RE +   YADVFKGD++WQ +  P  +T++W   STYI+  PYF GM+ +P  ++++  A+VLA LGD VTTDHISPA S K  TPA QYL    V  +++NS GSRRGNHEVMMRGTFANIR++N+++D + GGYT+      G QA I+DA + ++  G PLV+ AG++YG GSSRDWAAKGT LLGV+AVI E+FERIHRSNL+GMGV+P +F  G+S +SLGL G ET  I GL  +      + V  T T  +G   E     RIDT  E +Y  +GG+L YVLR ++ +
Sbjct:   32 MEKLPYSLKVLGENLLRTEDGA-NITEEHIEAIANW-DASADPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVKTLGGDPDKVNPLNPAEMVIDHSVIVEAFGRPDALEKNVEIEYERNEERYQFLRWGSEAFSNFRVVPPGTGIVHQVNIEYLARVVF---DNDG--LAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPVGVTATDVVLTITEMLRDHGVVQKFVEFYGNGVKSVPLANRATIGNMSPEFGSTCAMFPIDEETIKYLRLTGRPEEQIALVEAYAKAQGMWLEQD-APEAEYSEYLELDLSTVVPSIAGPKRPQDRILLSEAKEQFREDLKAYTNDPVQVDQSIPAKRMANEGGFQPGSTSDLDNYNASWPGEGESAAANAEGRPSNPVTVVSPQGGEYTIDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAEKGLKSKPWVKTICAPGSQVVDGYYQRADLWKDLEALGFYLSGFGCTTCIGNSGPLPEEISEAINEHDLAATAVLSGNRNFEGRISPDVKMNYLASPIMVIAYAIAGTMDFDFENEALGQDQDGNDVFLKDIWPSTEEIEETIQAAISRELYEADYADVFKGDKQWQELDIPSGKTFEWDENSTYIRKAPYFDGMTAEPQPVTDIENARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDAHGVERQDYNSLGSRRGNHEVMMRGTFANIRLQNQLVD-IAGGYTRDFTQEGGPQAFIYDACVNYKEAGIPLVVLAGKEYGTGSSRDWAAKGTNLLGVRAVITESFERIHRSNLIGMGVVPLQFPEGESHESLGLDGTETFDITGLTALNEGTTPKTVKVTATKENGEKVEFDAVVRIDTPGEADYFRHGGILQYVLRQMAAS 937          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|302595583|sp|A0QX20.1|ACNA_MYCS2 (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1008.05 bits (2605), Expect = 0.000e+0
Identity = 512/910 (56.26%), Postives = 653/910 (71.76%), Query Frame = 0

Query:   24 KLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREM-------------------AETF-----------------RRPMG---------KEVTVVGE---DYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG---PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTI---EPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            KLP +LKV+ EN+LR EDG  +++ D I+A + W D   +   EI + PARV+MQDFTGVP +VDLA MR+ + ALGGD  ++NPL P +LVIDHSV++D FGN  AF+ NV+ EYERN ERY FL+WGQ AF++F+VVPPGTGI HQVN+EYL++TV     ++GV  AYPDT VGTDSHTTMVNGL VLGWGVGGIEAEAAMLGQPVSMLIP VVGF+L+G++  G T TD+VL V +MLR HGVVGKFVEFYG G+  +PLA+RAT+ NM+PE+G+T   FPID ET+ YLR TGR ++++ALVEAYAK  G W D +  P+++  L LD+ T+VP+ISGPKRPQD + LT+AK AF +++                    E+F                  RP             V V  E   D+ ++ G VV+A ITSCTNTSNP VM+GA L+A+KA   GL  KPWVKT++APGSQVV++Y   AGL   L+K+G+ L GYGCTTCIGN+GP+ +E+S AI   DL  T+VLSGNRNFEGRISPDV+ NYLASPPLV+AY +AGTMD D  ++ LGQD  GNDVFL+DIWP++AEI   +  ++ RE F + YADVFKGD++W+++ TP+ +T++W  ASTY++  PYF GM  +P  +S++ GA+VLA+LGD VTTDHISPAG+ K  TPA QYL    V  +++NS GSRRGNHEVM+RGTFANIR++N++LD V GGYT+    P G QA I+DA+  ++  G PLV+  G++YG+GSSRDWAAKGT LLGVKAVI E+FERIHRSNL+GMGVIP +F  G+S  SL L G ET  I G++ +   +  + V  T T  DGS  E     RIDT  E +Y  NGG+L YVLRN+ K++
Sbjct:   40 KLPYSLKVLAENLLRTEDGA-NITKDHIEAIANW-DPNAEPSIEIQFTPARVIMQDFTGVPCIVDLATMREAVAALGGDPNKVNPLAPAELVIDHSVILDVFGNASAFERNVELEYERNAERYQFLRWGQGAFDDFKVVPPGTGIVHQVNIEYLARTVMV---RDGV--AYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLSGEIKPGVTATDVVLTVTDMLRRHGVVGKFVEFYGKGVAEVPLANRATLGNMSPEFGSTAAIFPIDEETINYLRLTGRTDEQLALVEAYAKAQGMWHDPEREPVFSEYLELDLSTVVPSISGPKRPQDRIELTDAKNAFRKDIHNYVEQNHPTPETKLDEAVEESFPASDPVSLSFADDGAPDMRPSAANGATGRPTNPVLVHSEERGDFVLDHGAVVVAGITSCTNTSNPSVMLGAALLAKKAVEKGLTTKPWVKTNMAPGSQVVTDYYNKAGLWPYLEKLGYYLGGYGCTTCIGNTGPLPEEISKAINDNDLAVTAVLSGNRNFEGRISPDVKMNYLASPPLVIAYGIAGTMDFDFESDPLGQDSEGNDVFLRDIWPSAAEIEETIASSINREMFTESYADVFKGDDRWRSLPTPEGDTFEWDPASTYVRKAPYFDGMPAEPEPVSDIKGARVLALLGDSVTTDHISPAGAIKPGTPAAQYLDANGVERKDYNSLGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTQPGGPQAFIYDASENYKKAGIPLVVLGGKEYGSGSSRDWAAKGTVLLGVKAVITESFERIHRSNLIGMGVIPLQFPAGESAASLKLDGTETYDIEGIEELNSGKTPKTVHVTATKEDGSKVEFDAVVRIDTPGEADYYRNGGILQYVLRNMLKSS 942          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1361156753|ref|WP_106353776.1| (aconitate hydratase AcnA [Loktanella maritima] >gi|1360119486|gb|PRY80321.1| aconitate hydratase [Loktanella maritima])

HSP 1 Score: 1662.51 bits (4304), Expect = 0.000e+0
Identity = 805/896 (89.84%), Postives = 840/896 (93.75%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            MT+TVG DTAKT                  A+AAGLGDFSKLPAALKVVLEN+LRFED GFSVSVDDIKAFSEWAD GGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDL+IDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYL+QTVWTD DQNGVE+AYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTG+M+EGTTGTDLVLKVVEMLR  GVVGKFVEF+G GLD LPLADRATIANMAPEYGATCGFFPID ETLRYLRNTGRDEDRIALVEAYAKENGFWRDADY PIYTA L LDMGTIVPAISGPKRPQDYVALT AKTAF  EM  TF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDI+LAT+ + QD+NGNDV+LKDIWPT+ EIN LVEKTVTREAF+KKYADVFKGDEKWQAVQTPDSETYDWPAASTY+QNPPYF+GMSKD G I N+  AKVLAVLGDMVTTDHISPAGSFKD+TPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ +IFDAAM +Q  GTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGV+PFEFTGGDSRK+LGLTG+ETV I GLD ++PLQ +TA ITMADG+ KEITVKCRIDTAVEIEYIENGGVLHYVLRNL+KA+
Sbjct:    1 MTITVGADTAKTRKTLSVGDQSVAYYSIPAAQAAGLGDFSKLPAALKVVLENLLRFEDDGFSVSVDDIKAFSEWADNGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLIIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLAQTVWTDKDQNGVEVAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGEMVEGTTGTDLVLKVVEMLRELGVVGKFVEFFGEGLDRLPLADRATIANMAPEYGATCGFFPIDNETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYNPIYTAKLHLDMGTIVPAISGPKRPQDYVALTGAKTAFANEMENTFKRPMGKEVPVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDINLATDPIAQDKNGNDVYLKDIWPTNQEINELVEKTVTREAFLKKYADVFKGDEKWQAVQTPDSETYDWPAASTYVQNPPYFRGMSKDAGTIKNVENAKVLAVLGDMVTTDHISPAGSFKDTTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQTSIFDAAMEYQEAGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVVPFEFTGGDSRKTLGLTGEETVTIEGLDAVKPLQEMTAQITMADGTTKEITVKCRIDTAVEIEYIENGGVLHYVLRNLAKAS 896          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1282008160|ref|WP_100367036.1| (aconitate hydratase AcnA [Loktanella maricola] >gi|1280215254|gb|PJI92194.1| aconitase [Loktanella maricola])

HSP 1 Score: 1660.2 bits (4298), Expect = 0.000e+0
Identity = 803/896 (89.62%), Postives = 842/896 (93.97%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            MT+TVG DTA T                  AEAAGLGDFSKLPAALKVVLEN+LRFEDGGFSVSVDDIKAFS WAD+GGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVWTD DQNG E+AYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTG+MMEGTTGTDLVLKVVEMLR  GVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPID ETLRYLRNTGRDEDRIALVEAYAKENGFWR ADYAP+YT TL LDMGTIVPAISGPKRPQDYVALTNAK+AFT+EM ETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVSEYLEAAGLQ+DLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDL++E LGQDQNGNDVFLKDIWPTSAEIN LVEKTVTREAFI KYADVFKGDEKWQAV+T   ETYDWPA STY+QNPPYFQGMSKD G+I+++  AKVLAVLGDMVTTDHISPAGSFKD+TPAGQYL+ERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ +IFDAAMAHQAEGTPLV+FAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGV+PFEFTGGD+R SLGLTG+ETV I GL  ++PLQ +TA ITM DG+ K+ITVKCRIDTAVEI+YI++GGVLHYVL+NL+KAA
Sbjct:    1 MTITVGKDTANTRKTLSVGDQSVAYYSIPAAEAAGLGDFSKLPAALKVVLENLLRFEDGGFSVSVDDIKAFSAWADQGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQGAFNNFRVVPPGTGICHQVNLEYLAQTVWTDKDQNGEEVAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGKMMEGTTGTDLVLKVVEMLRELGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDNETLRYLRNTGRDEDRIALVEAYAKENGFWRGADYAPVYTNTLHLDMGTIVPAISGPKRPQDYVALTNAKSAFTKEMEETFKRPMGKEVAVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSEYLEAAGLQDDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLSSEPLGQDQNGNDVFLKDIWPTSAEINELVEKTVTREAFISKYADVFKGDEKWQAVETSTGETYDWPATSTYVQNPPYFQGMSKDAGEINDVVDAKVLAVLGDMVTTDHISPAGSFKDTTPAGQYLLERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQTSIFDAAMAHQAEGTPLVVFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVVPFEFTGGDNRNSLGLTGEETVTIEGLSEVKPLQELTAKITMGDGTLKKITVKCRIDTAVEIDYIKSGGVLHYVLQNLAKAA 896          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|800969712|ref|WP_045996377.1| (aconitate hydratase AcnA [Loktanella sp. S4079] >gi|798773377|gb|KJZ20360.1| aconitate hydratase [Loktanella sp. S4079])

HSP 1 Score: 1649.41 bits (4270), Expect = 0.000e+0
Identity = 794/896 (88.62%), Postives = 839/896 (93.64%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            MT+TVG DTA T                  A+AAGLGDFSKLPAALKVVLEN+LRFEDGGFSVSVDDIKAF+EWA+KGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQNG+E+AYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTG+M+EGTTGTDLVLKVVEMLR  GVVGKFVEF+G GLD LPLADRATIANMAPEYGATCGFFPID ETLRYLRNTGRDEDRIALVEAYAKENGFWRD +YAPIYT  L LDMGTIVPAISGPKRPQDYVALT+AK+ F +EMAETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVSEYLEAAGLQ+DLDKIGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDL +E +GQD +GNDV+LKDIWPTSAEIN LVEKTVTREAFI KYADVFKGDEKWQ+V+T D ETYDWP +STY+QNPPYF+GMSKD G I N+  AKVLAVLGDMVTTDHISPAGSFKD+TPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ +IFDAAM +Q  GTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGV+PFEFTGGD+RK+LGLTGDETV+I GLD ++PLQ +TA ITMADGS KEITVKCRIDTAVEIEY+ENGGVLHYVLRNL+KA+
Sbjct:    1 MTITVGADTANTRKTLTVGSQSVAYYSIPAAQAAGLGDFSKLPAALKVVLENLLRFEDGGFSVSVDDIKAFAEWAEKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQGAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQNGIEVAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGEMLEGTTGTDLVLKVVEMLRELGVVGKFVEFFGEGLDRLPLADRATIANMAPEYGATCGFFPIDNETLRYLRNTGRDEDRIALVEAYAKENGFWRDENYAPIYTDQLHLDMGTIVPAISGPKRPQDYVALTDAKSEFAKEMAETFKRPMGKEVAVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSEYLEAAGLQDDLDKIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLTSEPIGQDTDGNDVYLKDIWPTSAEINELVEKTVTREAFISKYADVFKGDEKWQSVETSDGETYDWPPSSTYVQNPPYFRGMSKDAGTIDNVENAKVLAVLGDMVTTDHISPAGSFKDTTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQTSIFDAAMEYQDTGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVVPFEFTGGDTRKTLGLTGDETVSIKGLDAVKPLQEMTAQITMADGSTKEITVKCRIDTAVEIEYVENGGVLHYVLRNLAKAS 896          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1120025295|ref|WP_072899675.1| (aconitate hydratase AcnA [Loktanella sediminum] >gi|1109849080|emb|SHG79154.1| aconitase [Loktanella sediminum])

HSP 1 Score: 1622.45 bits (4200), Expect = 0.000e+0
Identity = 785/896 (87.61%), Postives = 830/896 (92.63%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            MTVTVG DTAKT                  AE AGLGDFSKLPAALKVVLEN+LRFED GFSVS DDIKAFS+WA  GGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDA+QINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQNG E+AYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTG+MMEGTTGTDLVLKVVEMLR  GVVGKFVEF+G GLD LPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRD +YAPIYT  L LDMGTIVPAISGPKRPQDYVALT+AK AF +EM +TF+R +GKE+ V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGS+VVSEYLEAAGLQ+DLDKIGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDL +EA+ QDQ+GNDVFLKDIWPTS EINALVE+TVTRE FI KYADVFKGDEKWQAV+T DS TYDWPAASTY+QNPPYFQGM    GKIS++  AKVLAVLGDM+TTDHISPAGSFKD+TPAGQYL++RQVP+REFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDG Q +IFDA+MAHQA GTPLVIF G QYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGD+RKSLGLTG+ETV I GL++++PLQ V A I MADGS KEIT+KCRIDTAVEI+YIENGGVLHYVLRNL+KAA
Sbjct:    1 MTVTVGKDTAKTRKSLKIGDKTIAYYSIKAAEEAGLGDFSKLPAALKVVLENLLRFEDDGFSVSTDDIKAFSDWAKNGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAEQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQGAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQNGEEVAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGEMMEGTTGTDLVLKVVEMLRELGVVGKFVEFFGEGLDRLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDENYAPIYTTKLHLDMGTIVPAISGPKRPQDYVALTDAKAAFRKEMEDTFKRLLGKEIDVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSKVVSEYLEAAGLQDDLDKIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLNSEAIAQDQDGNDVFLKDIWPTSDEINALVEQTVTREGFISKYADVFKGDEKWQAVETTDSLTYDWPAASTYVQNPPYFQGMGAQAGKISDVVNAKVLAVLGDMITTDHISPAGSFKDTTPAGQYLLDRQVPMREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGTQTSIFDASMAHQAAGTPLVIFGGVQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDTRKSLGLTGEETVTIEGLESVQPLQEVPAKIEMADGSVKEITLKCRIDTAVEIDYIENGGVLHYVLRNLAKAA 896          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|499359720|ref|WP_011048029.1| (aconitate hydratase AcnA [Ruegeria pomeroyi] >gi|56678908|gb|AAV95574.1| aconitate hydratase 1 [Ruegeria pomeroyi DSS-3])

HSP 1 Score: 1613.97 bits (4178), Expect = 0.000e+0
Identity = 777/896 (86.72%), Postives = 827/896 (92.30%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            M + VG DTAKT                  A  AGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAF+EW  KGG+NPREIAYRPARVLMQDFTGVPAVVDLAAMRDGI ALGGDAQ+INPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQ+GVE+AYPDTLVGTDSHTTMVNG+AVLGWGVGGIEAEAAMLGQP+SMLIPEV+GFELTGQM+EGTTGTDLVLKVVEMLRA GVVGKFVEFYG GLD LPLADRATIANMAPEYGATCGFFPID +TL Y+RNTGRDEDRIALVEAYAKENGFWRDADYAP+YT TLSLDMGTIVPAISGPKRPQDYVALT+AKTAFTREMAETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVS YLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVV YALAGT+DIDL +E +G D++GNDV++KDIWPT AEI  LV++TVTREAF  KYADVFKGDEKWQAV+T D+ETYDWPA STYIQNPPYFQGM  +PG ISN+SGAKVLA+LGDMVTTDHISPAGSF  +TPAG+YL ERQV  REFNSYGSRRGNHE+MMRGTFANIRIKNEMLDGVEGGYTKGPDG Q +I++A+MA+Q +GTPLV+F GEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGD+RKSLGL GDETVAI+GLDTI+PLQ V  TIT  DGS K IT+KCRIDTA EIEYIENGGVLHYVLRNL+KA+
Sbjct:    1 MPIKVGQDTAKTRRTLSVNGKSISYYSIPAATEAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFAEWGAKGGQNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIKALGGDAQKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQGAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQDGVEVAYPDTLVGTDSHTTMVNGMAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFELTGQMVEGTTGTDLVLKVVEMLRAKGVVGKFVEFYGTGLDRLPLADRATIANMAPEYGATCGFFPIDDKTLAYMRNTGRDEDRIALVEAYAKENGFWRDADYAPVYTDTLSLDMGTIVPAISGPKRPQDYVALTDAKTAFTREMAETFKRPMGKEVAVKGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSAYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVVYALAGTLDIDLTSEPIGTDKDGNDVYMKDIWPTQAEIAELVDRTVTREAFQSKYADVFKGDEKWQAVETTDAETYDWPATSTYIQNPPYFQGMGTEPGTISNISGAKVLAILGDMVTTDHISPAGSFATTTPAGKYLTERQVAPREFNSYGSRRGNHEIMMRGTFANIRIKNEMLDGVEGGYTKGPDGQQTSIYEASMAYQEQGTPLVVFGGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDNRKSLGLKGDETVAISGLDTIQPLQEVPCTITYGDGSEKTITLKCRIDTAPEIEYIENGGVLHYVLRNLAKAS 896          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|338757520|gb|AEI93984.1| (aconitate hydratase AcnA [Roseobacter litoralis Och 149])

HSP 1 Score: 1609.73 bits (4167), Expect = 0.000e+0
Identity = 768/896 (85.71%), Postives = 830/896 (92.63%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            M +TVG DT+KT                  A AAGLGDFSKLPAALKVVLENMLRFEDG  +V+VDDIKAF++W  +GGKNPREIAYRPARVL+QDFTGVPAVVDLAAMRD I++LGGDA++INPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQNG E+AYPDTLVGTDSHTTMVNG AVLGWGVGGIEAEAAMLGQP+SMLIPEV+GFELTG MMEGTTGTDLVLKVVE+LRA GVVGKFVEFYG GLD LPLADRATIANMAPEYGATCGFFPID ETLRYLRNTGRDEDR+ LVEAYAKENGFWRDADYAPIYT TL LDMGTIVPAISGPKRPQD+VALT+ +TAF +EM ETF+RPMGKEV V GE+YTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVS YLEAAGLQ+DLD IGFNLVGYGCTTCIGNSGP+QKE+S+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGT+DI+LAT+A+GQDQNGNDVFLKDIWP+S E+  LVE+TVTREAF+ KYADVFKGDEKWQ V T DS+ YDWP +STY+QNPPYFQGM ++PG ISN+  A VLA+LGDM+TTDHISPAGSFKD+TPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGP+G QA+I+DAAM HQ  GTPLVIF GEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGD+RKSLGLTGDE V+I+GLDTI+PLQ VT TITMAD ++K+IT+KCRIDTA+EIEYIE+GGVLHYVLRNL+KAA
Sbjct:    1 MPITVGHDTSKTRKTLSAGAQTIDYYSIPAATAAGLGDFSKLPAALKVVLENMLRFEDGK-TVTVDDIKAFAQWGAQGGKNPREIAYRPARVLLQDFTGVPAVVDLAAMRDAIISLGGDAEKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQNGAEVAYPDTLVGTDSHTTMVNGAAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFELTGTMMEGTTGTDLVLKVVELLRAKGVVGKFVEFYGEGLDRLPLADRATIANMAPEYGATCGFFPIDAETLRYLRNTGRDEDRVVLVEAYAKENGFWRDADYAPIYTDTLHLDMGTIVPAISGPKRPQDFVALTDGQTAFRKEMEETFKRPMGKEVAVAGEEYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSAYLEAAGLQDDLDSIGFNLVGYGCTTCIGNSGPLQKEISEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTLDINLATDAIGQDQNGNDVFLKDIWPSSQEVAELVERTVTREAFLTKYADVFKGDEKWQGVSTTDSKVYDWPVSSTYVQNPPYFQGMGQEPGTISNIENASVLAILGDMITTDHISPAGSFKDTTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPNGEQASIYDAAMTHQEAGTPLVIFGGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDTRKSLGLTGDEKVSISGLDTIQPLQEVTCTITMADRTSKQITLKCRIDTAIEIEYIEHGGVLHYVLRNLAKAA 895          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|742895568|ref|WP_039018181.1| (aconitate hydratase AcnA [Halocynthiibacter namhaensis])

HSP 1 Score: 1606.65 bits (4159), Expect = 0.000e+0
Identity = 774/895 (86.48%), Postives = 829/895 (92.63%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
            M +TVG DTAKT                  A+AAGLGDFSKLPAALKVVLENMLRFEDG  +VSVDDIKAF+EW   GGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDA++INPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERYTFLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQ+G E+AYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQP+SMLIPEVVGFELTG+MMEGTTGTDLVLKVVE+LRA GVVGKFVEFYG GLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLR +GRDEDRIALVEAYAKENGFWRDADYAP+YT TL LDMGTIVPAISGPKRPQDYVALT  + AF REM ETF+RPMGKEV V GEDYT+ESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVS YLEAA LQ+DLD IGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGT+DI+LAT+ + QD++GNDV+LKDIWPT+ E+  LVE+TVTREAF++KYADVFKGD+KWQ V+T DS+TYDWP+ASTY+QNPPYFQGM+ D  +I+N+ GAKVLAVLGDMVTTDHISPAGSFKD+TPAGQYL++RQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEML GVEGGYT GPDGAQ +I+DAAMAHQ  GTPLVIF GEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFT GDSRKSLGLTG+ETVAI GLDTI+PLQ+V   ITMADGS K IT+KCRIDTAVEIEYIENGGVLHYVLRNL+K+
Sbjct:    1 MPITVGQDTAKTRKTLQVGNQSISYYSIPAAQAAGLGDFSKLPAALKVVLENMLRFEDGK-TVSVDDIKAFAEWGANGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAEKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYTFLKWGQSAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQDGNEVAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPISMLIPEVVGFELTGEMMEGTTGTDLVLKVVELLRAKGVVGKFVEFYGKGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRTSGRDEDRIALVEAYAKENGFWRDADYAPVYTDTLHLDMGTIVPAISGPKRPQDYVALTEGQNAFRREMEETFKRPMGKEVAVAGEDYTLESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSAYLEAADLQKDLDSIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTLDINLATDVIAQDKDGNDVYLKDIWPTTQEVAELVEQTVTREAFLEKYADVFKGDDKWQGVETTDSQTYDWPSASTYVQNPPYFQGMTMDTKEITNIEGAKVLAVLGDMVTTDHISPAGSFKDTTPAGQYLVDRQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLPGVEGGYTNGPDGAQTSIYDAAMAHQEAGTPLVIFGGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTSGDSRKSLGLTGEETVAIKGLDTIKPLQDVPCEITMADGSVKAITLKCRIDTAVEIEYIENGGVLHYVLRNLAKS 894          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|496448447|ref|WP_009157292.1| (aconitate hydratase AcnA [Thalassobium sp. R2A62] >gi|255103542|gb|EET46216.1| aconitate hydratase 1 [Thalassobium sp. R2A62])

HSP 1 Score: 1605.11 bits (4155), Expect = 0.000e+0
Identity = 774/894 (86.58%), Postives = 824/894 (92.17%), Query Frame = 0

Query:    3 VTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            +TVG DTAKT                  A AAGLGDF++LPAALKVVLENMLRFEDG  +VSVDDIKAF+EW  KGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGD +QINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERYTFLKWGQ AFNNFRVVPPGTGICHQVNLEYLSQTVWTD DQNG E+AYPDTLVGTDSHTTMVNG AVLGWGVGGIEAEAAMLGQP+SMLIPEV+GFELTG+MMEGTTGTDLVLKVVEMLRA GVVGKFVEF+GAGLD LPLADRATIANMAPEYGATCGFFPID ETLRYLR TGRDEDRIALVEAYAKENGFWRDADYAPIYT TL LDM +IVPAISGPKRPQDYVALTNAK AF +EM  TF RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVS YLEAA LQ+DLD +GFNLVGYGCTTCIGNSGPIQKELSDAIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYA+AGT+DI+LAT+ +GQD++GNDV+L+DIWPTS E+  LVE+TVTR AF++KYADVFKGDEKWQ V+T D++TYDWPAASTY+QNPPYFQG++ D  +I+N+ GAKVLAVLGDM+TTDHISPAGSFKD+TPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ++IFDAAMAHQ  GTPLVIF GEQYGAGSSRDWAAKGTALLGVKAVIAE FERIHRSNLVGMGVIPFEFTGGD+RKSLGLTGDETV+I GLDTI+PLQ V   ITM DG+ KEI +KCRIDT +EIEYIENGGVLHYVLRNL+KAA
Sbjct:    2 ITVGQDTAKTRKTLTVGDASIAYYSIPAATAAGLGDFARLPAALKVVLENMLRFEDGK-TVSVDDIKAFAEWGAKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDPEQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYTFLKWGQSAFNNFRVVPPGTGICHQVNLEYLSQTVWTDKDQNGDEVAYPDTLVGTDSHTTMVNGAAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFELTGEMMEGTTGTDLVLKVVEMLRAKGVVGKFVEFFGAGLDTLPLADRATIANMAPEYGATCGFFPIDNETLRYLRTTGRDEDRIALVEAYAKENGFWRDADYAPIYTDTLHLDMASIVPAISGPKRPQDYVALTNAKEAFHQEMKNTFDRPMGKEVAVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSAYLEAAELQDDLDALGFNLVGYGCTTCIGNSGPIQKELSDAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYAIAGTLDINLATDVIGQDKDGNDVYLRDIWPTSQEVAELVEQTVTRSAFLEKYADVFKGDEKWQGVETTDAKTYDWPAASTYVQNPPYFQGITMDTKEITNIEGAKVLAVLGDMITTDHISPAGSFKDTTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQSSIFDAAMAHQEAGTPLVIFGGEQYGAGSSRDWAAKGTALLGVKAVIAETFERIHRSNLVGMGVIPFEFTGGDTRKSLGLTGDETVSIKGLDTIQPLQEVPCEITMTDGTVKEIMLKCRIDTGIEIEYIENGGVLHYVLRNLAKAA 894          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1226714976|ref|WP_093967135.1| (aconitate hydratase AcnA [Confluentimicrobium lipolyticum] >gi|1219650458|emb|SMX42233.1| Aconitate hydratase 1 [Confluentimicrobium lipolyticum])

HSP 1 Score: 1604.73 bits (4154), Expect = 0.000e+0
Identity = 776/896 (86.61%), Postives = 832/896 (92.86%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLD-TIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
            MT+TVG DT+KT                  AE AGLGDFSKLPAALKVVLENMLRFEDG  +VSVDDIKAF+EWA KGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQ+INPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYL+QTVWTD DQNG  +AYPDTLVGTDSHTTMVNG AVLGWGVGGIEAEAAMLGQP+SMLIPEV+GFELTG+MMEGTTGTDLVLKVVEMLRA GVVGKFVEFYG GLD LPLADRATIANMAPEYGATCGFFPID ET+RYL NTGRD+DRIALVEAYAKENGFWRDADYAPIYT TL+LDMGTIVPAISGPKRPQDYVALT AKTAF +EM ETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVS YLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGT+DI+LAT+ + QD++GNDV+LKDIWPT+ EI  LV +TVTREAF+ KYADVFKGDEKWQ V+T DS+TYDWPAASTY+QNPPYFQGMSK+PG I+++ GAKVLA+LGDM+TTDHISPAGSFK+STPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ +IFDAAMA+Q  GTPLVIF GEQYGAGSSRDWAAKGTALLGVKAV+AE+FERIHRSNLVGMGVIPFEFTGGD+RKSLGLTGDETV+I GL+  ++PL  V  TIT ADGS K+I++KCRIDTA+E EY+E+GGVLHYVLR+L+K+
Sbjct:    1 MTITVGHDTSKTRKTLSVGDQSVAYYSIKAAEEAGLGDFSKLPAALKVVLENMLRFEDGK-TVSVDDIKAFAEWAVKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLAQTVWTDVDQNGENVAYPDTLVGTDSHTTMVNGAAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFELTGEMMEGTTGTDLVLKVVEMLRAKGVVGKFVEFYGEGLDHLPLADRATIANMAPEYGATCGFFPIDAETIRYLDNTGRDKDRIALVEAYAKENGFWRDADYAPIYTDTLTLDMGTIVPAISGPKRPQDYVALTEAKTAFHKEMEETFKRPMGKEVPVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSAYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTLDINLATDPIAQDKDGNDVYLKDIWPTNKEIADLVHETVTREAFLSKYADVFKGDEKWQGVETTDSQTYDWPAASTYVQNPPYFQGMSKEPGVINDIKGAKVLALLGDMITTDHISPAGSFKESTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQTSIFDAAMAYQDAGTPLVIFGGEQYGAGSSRDWAAKGTALLGVKAVVAESFERIHRSNLVGMGVIPFEFTGGDTRKSLGLTGDETVSIEGLEGDLKPLSEVPCTITFADGSEKKISLKCRIDTAIEKEYVEHGGVLHYVLRDLAKS 895          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1189856150|ref|WP_085865525.1| (aconitate hydratase AcnA [Pseudooctadecabacter jejudonensis] >gi|1187966602|emb|SLN59191.1| Aconitate hydratase 1 [Pseudooctadecabacter jejudonensis])

HSP 1 Score: 1604.34 bits (4153), Expect = 0.000e+0
Identity = 776/893 (86.90%), Postives = 823/893 (92.16%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLS 876
            M +TVG D+AKT                  A+ AGLGDFS LPAALKVVLENMLRFEDG  +VSVDDIKAF+EW  KGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERYTFLKWGQ AFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNG E+AYPDTLVGTDSHTTMVNG AVLGWGVGGIEAEAAMLGQP+SMLIPEVVGFELTG MMEGTTGTDLVLKVVEMLRA GVVGKFVEFYGAGLD LPLADRATIANMAPEYGATCGFFPID ETLRYLRNTGRDEDRIALVEAYAKENGFWR  DYAP+YT TL LDMG IVPAISGPKRPQDYVALTNAK+AF +EM ETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVS YLEAAGLQ+DLDKIGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDI+LAT+ + Q  +G DV+LKDIWPTS E+  LVE TVTREAF  KYADVFKGDEKWQ VQT D+ETYDWPA STYIQNPPYFQGMSK+PG I+N+ GA+VLA+LGDM+TTDHISPAG+FK++TPAGQYL++RQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ +IF+AAMA+Q  G P VIF GEQYGAGSSRDWAAKGT LLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGD+RKSLGLTGDETV+I+GLDT++PLQ V  TITMADGS KEIT+KCRIDTA+EIEYIE+GGVLHYVLRNL+
Sbjct:    1 MPITVGHDSAKTRKTLTVGDQTLAYYSIPAAQDAGLGDFSNLPAALKVVLENMLRFEDGK-TVSVDDIKAFAEWGAKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYTFLKWGQSAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGEEVAYPDTLVGTDSHTTMVNGAAVLGWGVGGIEAEAAMLGQPISMLIPEVVGFELTGAMMEGTTGTDLVLKVVEMLRAKGVVGKFVEFYGAGLDSLPLADRATIANMAPEYGATCGFFPIDNETLRYLRNTGRDEDRIALVEAYAKENGFWRGDDYAPVYTDTLHLDMGAIVPAISGPKRPQDYVALTNAKSAFAKEMEETFKRPMGKEVAVAGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSAYLEAAGLQDDLDKIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDINLATDPIAQTPDGKDVYLKDIWPTSQEVAELVEATVTREAFQSKYADVFKGDEKWQGVQTTDAETYDWPAQSTYIQNPPYFQGMSKEPGVITNIEGARVLALLGDMITTDHISPAGAFKETTPAGQYLVDRQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQTSIFEAAMAYQDAGVPTVIFGGEQYGAGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDTRKSLGLTGDETVSISGLDTVQPLQEVPCTITMADGSVKEITLKCRIDTAIEIEYIEHGGVLHYVLRNLA 892