myosin heavy muscle isoform x20, snap_masked-scaffold286_size222086-processed-gene-1.7 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000012294 (supercontig:LSalAtl2s:LSalAtl2s899:154040:163234:-1 gene:EMLSAG00000012294 transcript:EMLSAT00000012294 description:"maker-LSalAtl2s899-snap-gene-1.28") HSP 1 Score: 2855.47 bits (7401), Expect = 0.000e+0 Identity = 1437/1767 (81.32%), Postives = 1607/1767 (90.95%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RRSECPPHIFGVAEGSYQGM+N KNQSILITGESGAGKTENTKKVISYFAS+GASGK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMV YLLEKSRLTFQAELERCYH+FYNLMSDAVPDLK+KCLLS++IYDYWWVSQGKV+V SIDDKEDMQ+A +A+ ILGF+ +E Y+VYKLTSVVMHMGNMTKDFVPVGK+EQAEIK + N+ KVA +CGID EWMI YFCKPKLKVGTEWV+KGQ+C+ A+SSVAGI+RKIYEL FRFI DKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCI MFEKPMGLL+ILEEESLFPKATD +FAAKLHENLL KC NFQK SP+PDP+AHFAV+HYAATVSYNLTSWLEKNKDPLNDTVVEL KNGSN LL++CF DHPGQPLEAKKDAGGGGRKKGGGKTVSSFYK QLDDLMK LY+TDPSFIRCVVPNTHK+PG VESGLVMHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNIL AA VAKAK DK AA AVL ++KLE EKFRLGHTKVFFRAGILGYMEE+REDR+G VLSWLQ+QARGKASR+VFKKMQDQKLALY QRTIRN++IG+TWLWWQ+WLL+KPNLKCTKFSQYKAEYEEKIAIAE NI KALADRKKVE ++ LL QK+EL LAL+SGGS VQDIIDK R+EGMAADVQKQL +VNNRI EK Q +S+ Q Q KV + L EI +E+ L +AEQD+ +KD+QIRTLKEE+EHQ +MI KL REK+ V ++KQKTEED+QA+ED+ +HLS+VK KLEQSLDEAED+LE EKK K D EK+KRK+E DLKLTQET+SDLERVKAELSQ+VQRK+KE +A++AKI+DE+TLG KYSKQ KELQSRLEELDEEL+IER +RAKAEKSRS+LKKDLEDLG+RLEEAG NTATQVELNKKRE EL R+K E+EE NI HEGTLAALRMKHNNTM+ELGEQID LN NK K+EKDK NMERDLQE RSSLEDGVRAKAE+D+NGKMIQGSI D++ KLDEMARALNEAESQKKRL++EK DLERQIEEGENAMA FNKQKISLTTQLEDTKRLADAEARDRSSLLTK+KN TT+LE++RERIE+EH+RKSD LKALSKAQAEIQLW+SR+ETEGMGRV+ELE +R KLQARI EAEETVE+L KI ++EKS++R+ DLEE+S+EYERTHAAAII+EKRG+NFD+VI EWK K +D++ E+EAS ECRNYNSELFRLRAA +E +EQLD+VKRENKNLADEI+DLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEA LEQEENKVLR+QLELGQVRQEIDR+IQEKEEEFNNTRKNHQRAMDSL ASLEAEQRAK+EALRIKKKLE DINELEIALD ANKAN+EGQKA+KRYQ LRD IQ YEDE R +Q V E VGI +RK NAL+GEVEESRALL S+ERSKRQ+D+EL ++R +VNEM IN+++MHEKR ES IH +QAEID++++ AKN+EEK+KRAMVDA+RLADELRSEQ+H+ SEDR KR+LESQL ELE+RL DAE +A KMG+ AMSKLEM+IRELE+ELGSIQ++T E+ KAYQR+ERH+KELQFQQ+EDRKNQ+RM++LA KLQQKIKTYKQQIEEAEEIAALNLAK+RK+QQE EE+E+R K A+ S
Sbjct: 128 RRSECPPHIFGVAEGSYQGMLNAAKNQSILITGESGAGKTENTKKVISYFASIGASGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVTYLLEKSRLTFQAELERCYHSFYNLMSDAVPDLKEKCLLSNDIYDYWWVSQGKVTVPSIDDKEDMQFADEAYDILGFSSQEKYDVYKLTSVVMHMGNMTKDFVPVGKDEQAEIKDDINSQKVATLCGIDCEWMITYFCKPKLKVGTEWVSKGQSCTGAASSVAGISRKIYELAFRFIVDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCITMFEKPMGLLSILEEESLFPKATDATFAAKLHENLLGKCENFQKPSPRPDPNAHFAVIHYAATVSYNLTSWLEKNKDPLNDTVVELMKNGSNALLVQCFIDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKAQLDDLMKVLYSTDPSFIRCVVPNTHKQPGMVESGLVMHQYQCNGVLAGIAICRKGFPNKMIYPEFKARYNILGAAVVAKAKNDKSAAGAVLKLIKLEPEKFRLGHTKVFFRAGILGYMEEIREDRIGAVLSWLQAQARGKASRLVFKKMQDQKLALYCLQRTIRNWHIGRTWLWWQIWLLLKPNLKCTKFSQYKAEYEEKIAIAEANIDKALADRKKVEAVNNALLAQKNELVLALQSGGSVVQDIIDKTNRIEGMAADVQKQLIDVNNRIKGEKAQSESIEQAQAKVNKEIGTLGNEIQNLESHLNSAEQDRSDKDDQIRTLKEEIEHQNDMINKLQREKKNVGESKQKTEEDIQAMEDRCNHLSRVKGKLEQSLDEAEDSLEREKKAKGDVEKLKRKVEGDLKLTQETLSDLERVKAELSQSVQRKDKEISAISAKIEDESTLGSKYSKQIKELQSRLEELDEELLIERQNRAKAEKSRSILKKDLEDLGARLEEAGCNTATQVELNKKRESELGRLKGELEEMNISHEGTLAALRMKHNNTMAELGEQIDGLNVNKTKSEKDKSNMERDLQECRSSLEDGVRAKAEMDKNGKMIQGSIVDSHQKLDEMARALNEAESQKKRLQVEKQDLERQIEEGENAMATFNKQKISLTTQLEDTKRLADAEARDRSSLLTKFKNCTTDLENTRERIEDEHQRKSDALKALSKAQAEIQLWRSRYETEGMGRVDELESSRNKLQARITEAEETVESLQCKIANNEKSKNRMHSDLEEISMEYERTHAAAIITEKRGKNFDKVINEWKCKGEDISSELEASQNECRNYNSELFRLRAASDEVVEQLDIVKRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAXLEQEENKVLRAQLELGQVRQEIDRRIQEKEEEFNNTRKNHQRAMDSLGASLEAEQRAKSEALRIKKKLESDINELEIALDHANKANSEGQKAIKRYQCNLRDIIQSYEDECRSKQHVMEQVGICERKSNALSGEVEESRALLDSAERSKRQIDSELSDARNTVNEMQVINSKSMHEKRNVESIIHTLQAEIDEVLSSAKNAEEKSKRAMVDASRLADELRSEQDHTGSEDRAKRSLESQLSELESRLHDAETSAMKMGKDAMSKLEMKIRELELELGSIQTKTQENYKAYQRSERHIKELQFQQDEDRKNQDRMSDLASKLQQKIKTYKQQIEEAEEIAALNLAKYRKAQQELEETEERCKLADTS 1894 HSP 2 Score: 473.396 bits (1217), Expect = 7.126e-137 Identity = 206/231 (89.18%), Postives = 219/231 (94.81%), Query Frame = 0
Query: 33 PYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
PYD KKSCWVP+K +GGY EGLI+STDGDKVTV I ++ D KVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACV W+SVVRYKNELIYTYSGLFCIAINPYKRFPIYTQR++++YIGKRRSECPPHIFGVAEGSYQGM+N KNQSILITGESGAGKTENTKKVISYFAS+GASGK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM
Sbjct: 10 PYDAKKSCWVPEKGTGGYLEGLIESTDGDKVTVKIKDSGDTKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVXWBSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRAMEIYIGKRRSECPPHIFGVAEGSYQGMLNAAKNQSILITGESGAGKTENTKKVISYFASIGASGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 240 HSP 3 Score: 60.077 bits (144), Expect = 8.343e-9 Identity = 219/964 (22.72%), Postives = 392/964 (40.66%), Query Frame = 0
Query: 1188 AELSQAVQRKEKEWAAMNAKIDDENTL------GGKYSKQTKELQSRLEELDEEL---MIERGSRAKAEKSRS--------MLKKDLEDLGSRLE--EAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKD--------------KGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLA---HEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVK-------RENKNLAD-EIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLE---LGQV------RQEIDRKIQEKEEEF--------NNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKK---LEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFR---QEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDA-----ELEESRGSVNEMSSINTRAMHEKRATESGIH--AIQAEIDDMMTQAK----NSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIREL-------------EMELGSIQSRTS--------ESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQK-------LQQKIKTYKQQIEE-------AEE------IAALNLAKFRKSQQEFEESEDRSKTAEKSPI 2032
A + +A+ ++K A NA + +N L GG + + +R+E + ++ +I+ +R K EK++S + K++ LG+ ++ E+ N+A Q +K + ++ +K EIE +N + +++L + N+ +K K E+D KG +E+ L EA SLE +AK ++++ + ++G + L ++ R E +R + E + +IE+ ++++KQ L ++LE+ E ++R+ L +LE R+E + ++ K ++E+ K E + L R K + E E ++ LN SEK +S ++ DL+E E A +K G+ I + K D++A +E E+ K + +L R E A+ + K + K LAD E RD L +L+ R R+E E + AL+ A Q E ++ RS+ E +G+V R ++ +I E EE NN + ++ D S+E E R A+ +K+ + INE + + + Q + Y +L R R EV E + I R+ LA E+++ L RS +LD E+E+ + E + + + ++ EID + + + N+ + +RAM L L +EQ + R K+ LES + ELE L D A G+ A+ + + +R++ ME I R S ES AER +++ + + R M + K ++ I T + +I+E AEE + A LA +S+Q+ SEDR+K + +S +
Sbjct: 839 ANIDKALADRKKVEAVNNALLAQKNELVLALQSGGSVVQDIIDKTNRIEGMAADVQKQLIDVNNRIKGEKAQSESIEQAQAKVNKEIGTLGNEIQNLESHLNSAEQDRSDK--DDQIRTLKEEIEHQN---------------DMINKLQREKKNVGESKQKTEEDIQAMEDRCNHLSRVKGKLEQSLDEAEDSLEREKKAKGDVEKLKRKVEGDLKLTQETLSDLERVKAELSQSVQRKDKEISAISAKIEDESTLGSKYSKQIKELQSRLEELDEELLIERQNRAKAEKSRSILKKDLEDLGARLEEAGCNTATQVELNKKRESELGRLKGELEEMNISHEGTLAALRMKHNNTMAELGEQIDGLNVNKTKSEKDKSNMERDLQECRSSLEDGVRAKAEMDKNGKMIQGSIVDSHQKLDEMARALNEAESQKKRLQVEKQDLERQIEEGENAMATFNKQKISLTTQLEDTKRLADAEARDRSSLLTKFKNCTTDLENTRERIEDEHQRKSDALKALSKA--QAEIQLWRSRYETEGMGRVDELESSRNKLQARITEAEETVESLQCKIANNEKSKNRMHSDLEEISMEYE-RTHAAAIITEKRGKNFDKVINEWKCKGEDISSELEASQNECRNYNSEL----------FRLRAASDEVVEQLDIVKRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAXLEQEENKVLRAQLELGQVRQEIDRRIQEKEEEFNNTRKNHQRAM---DSLGASLEAEQRAKSEALRIKKKLESDINELEIAL-DHANKANSEGQKAIKRYQCNLRDIIQSYEDECRSKQHVMEQVGICERKSNALSGEVEESRALLDSAERSKRQIDSELSDARNTVNEMQVINSKSMHEKRNVESIIHTLQAEIDEVLSSAKNAEEKSKRAMVDASRLADELRSEQDHTGSEDRAKRSLESQL 1768
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000004753 (supercontig:LSalAtl2s:LSalAtl2s250:270217:279770:-1 gene:EMLSAG00000004753 transcript:EMLSAT00000004753 description:"maker-LSalAtl2s250-snap-gene-3.17") HSP 1 Score: 2790.37 bits (7232), Expect = 0.000e+0 Identity = 1402/1775 (78.99%), Postives = 1590/1775 (89.58%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIR--------DLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RRSECPPHIFGVAEGSYQGM+N GKNQSILITGESGAGKTENTKKVISYFAS+GASGK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMV+YLLEKSRLTFQAELERCYHAFYN+MSDAVP+LK+ CLLS++IYDYWWVSQGKV+V SIDDKEDMQ+A +A+ ILGF++EE ++VYKLTSVVMHMGNMTKDFVPVGKEEQAEIK + N++KVA +CGIDSEWM YFCKPKLKVG EWV+KGQTCS A+SSVAGI RKIYEL FRFI +KCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCI MFEKPMGLL+ILEEESLFPKATDQ+FAAKLHENLL KC NFQK +P+PDP+AHFAV+HYAATVSYNLT+WLEKNKDPLNDT+VELFKNGSN LL+ECFRDHPGQP+EAKKD+GG + G KTVSSFYK QLDDLMKTLYATDP+FIRCVVPNTHK+PGGVE GLVMHQYQCNGVLAGIAICRKGFPNKM Y +FK RYNILAA AVAKAK DK AA AVL +KL+AEKFRLGHTKVFFRAGILGYMEE+RED++G VLSWLQ+QARGK SR+VFKKMQDQKLALY CQRTIRN++IGKTWLWWQ+WL +KPNLKCTKFSQYKAEYEEKIAIAE NI KALA+RKKVE ++ LL QK+EL LAL+SGGSAVQDIIDK R+E MAADVQKQLD+ NNRI EK Q++S+ Q Q KV + + L +EI +E +L AEQD+ +KD+QIRTL+EE+EHQ +MI KL REK+ V D+KQKTEED+QA+ED+ +HLSKVK KLEQ+LDEAED+LE EKK K D EK+KRK+E DLKLTQET+SDLERV+AEL+Q+VQRK+KE +A++AKI+DE+TLG KY KQ KELQSR+EELDEEL+IER +R+KAEK+RS+LKKD+EDLGSRLEEAGA+TATQVELNKKRE EL R+K+E+EE I EGTLAALRMKHNNTM+ELGEQID LN NKMK+EKDK NMERDLQEARS+LE+GVR KAE+D+NGK+IQGSI DAN KLDE+ARALNE +SQKKRL++EK DLERQI+EGENAMA NKQKISLT Q ED KR+AD EARD SSLLTK+KNLTT+LE+ +ERIE+EH+RKSD LKALSKAQAE QLW+SR+ETEGMGRVEELEG+RGKLQARI EAEETVE+L +KI + EKS++R+ DL+++S+EYERTHAAAII+EKRG+NFD+VI EWK K DD+++E++AS KECRNYNSELFRLRAA + +EQLD+VKRENKNLADEI+ DLLDQLGDGGRSIHELDKQRRRLEVEKEE QAALEEAEAALEQEENKVLR+QLELGQ +QEID KIQEKE+ FNNTRKNHQRAMDSLSASLEAEQ+AK+EALRIKKKLE DINELEIALD ANKAN+EGQKA+KRYQ LRDTIQ YED+ RQE+ E VGI DRK NAL+GE+EESRALL SSERSKRQLD EL +SR + NEM IN++AMHEKR ES IH +QAEID++++QAKNSEEK+KRAM+DAARLADELR+EQEH+T+ DR RAL SQL ELE RL DAE + K G+ +SKLEM+IRELE+ELGS+QSRT E+ KAYQR+ER +KELQFQQEEDR NQ++M++LA KLQQKIKTYKQQIE AEEIAALNLAK+RK+QQE EE+E+R K A S
Sbjct: 120 RRSECPPHIFGVAEGSYQGMLNAGKNQSILITGESGAGKTENTKKVISYFASIGASGKKKEGEVGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVVYLLEKSRLTFQAELERCYHAFYNIMSDAVPELKENCLLSNDIYDYWWVSQGKVTVPSIDDKEDMQFADEAYDILGFSQEEKFDVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKDDTNSIKVATLCGIDSEWMNTYFCKPKLKVGMEWVSKGQTCSGAASSVAGIGRKIYELTFRFIVEKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCITMFEKPMGLLSILEEESLFPKATDQTFAAKLHENLLGKCENFQKPNPRPDPNAHFAVIHYAATVSYNLTAWLEKNKDPLNDTIVELFKNGSNKLLVECFRDHPGQPIEAKKDSGGRKKGGG--KTVSSFYKTQLDDLMKTLYATDPAFIRCVVPNTHKQPGGVEPGLVMHQYQCNGVLAGIAICRKGFPNKMVYPEFKNRYNILAAQAVAKAKNDKXAAAAVLKSIKLDAEKFRLGHTKVFFRAGILGYMEEIREDKIGAVLSWLQAQARGKTSRLVFKKMQDQKLALYCCQRTIRNWHIGKTWLWWQVWLFLKPNLKCTKFSQYKAEYEEKIAIAEANIDKALAERKKVEVVNSSLLNQKNELVLALQSGGSAVQDIIDKTVRIEAMAADVQKQLDDCNNRIKGEKTQKESIEQAQSKVSIEMNSLGDEIKNLEDKLGNAEQDRSDKDDQIRTLREEIEHQNDMIQKLHREKKNVGDSKQKTEEDIQAMEDRCNHLSKVKGKLEQALDEAEDSLEREKKCKGDIEKLKRKVEGDLKLTQETVSDLERVQAELNQSVQRKDKELSALSAKIEDESTLGSKYGKQIKELQSRMEELDEELIIERQNRSKAEKNRSILKKDIEDLGSRLEEAGASTATQVELNKKREAELGRLKSELEEMTIAQEGTLAALRMKHNNTMAELGEQIDGLNNNKMKSEKDKANMERDLQEARSNLEEGVRGKAEIDKNGKLIQGSIVDANQKLDELARALNEGDSQKKRLQVEKADLERQIDEGENAMASLNKQKISLTXQFEDNKRIADGEARDCSSLLTKFKNLTTDLENIKERIEDEHQRKSDCLKALSKAQAETQLWRSRYETEGMGRVEELEGSRGKLQARIQEAEETVESLQSKISNGEKSKNRMXADLDDISMEYERTHAAAIITEKRGKNFDKVINEWKCKGDDISNELDASEKECRNYNSELFRLRAAQNDVVEQLDIVKRENKNLADEIKDLLDQLGDDLLDQLGDGGRSIHELDKQRRRLEVEKEEFQAALEEAEAALEQEENKVLRAQLELGQAKQEIDHKIQEKEDIFNNTRKNHQRAMDSLSASLEAEQKAKSEALRIKKKLESDINELEIALDHANKANSEGQKAIKRYQSNLRDTIQAYEDQCHHRQEIMENVGICDRKANALSGELEESRALLNSSERSKRQLDTELVDSRNTTNEMQVINSKAMHEKRNVESIIHTLQAEIDEVLSQAKNSEEKSKRAMIDAARLADELRAEQEHTTNGDRCNRALGSQLSELENRLIDAENASMKSGKEILSKLEMKIRELEIELGSVQSRTQENYKAYQRSERKIKELQFQQEEDRNNQDKMSDLASKLQQKIKTYKQQIEXAEEIAALNLAKYRKAQQELEETEERCKMANTS 1892 HSP 2 Score: 478.019 bits (1229), Expect = 2.280e-138 Identity = 216/263 (82.13%), Postives = 223/263 (84.79%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKKS+GPDPDP WLIVS ELK KLKSKPYD KKSCW KVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQR++D+YIGKRRSECPPHIFGVAEGSYQGM+N GKNQSILITGESGAGKTENTKKVISYFAS+GASGK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM
Sbjct: 1 MPGHIKKSEGPDPDPDQWLIVSDELKVKLKSKPYDAKKSCWT-------------------------------KVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRAMDIYIGKRRSECPPHIFGVAEGSYQGMLNAGKNQSILITGESGAGKTENTKKVISYFASIGASGKKKEGEVGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 232
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000007683 (supercontig:LSalAtl2s:LSalAtl2s447:396017:402225:-1 gene:EMLSAG00000007683 transcript:EMLSAT00000007683 description:"maker-LSalAtl2s447-augustus-gene-4.41") HSP 1 Score: 2683.29 bits (6954), Expect = 0.000e+0 Identity = 1354/1765 (76.71%), Postives = 1546/1765 (87.59%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+ECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQ GKLSGADMV YLLEKSRLTFQAELERCYH+FYNLMSDAVPDLK+KCLLS+NI+DYWWVSQGKV+V SIDDKEDMQ+A +AF ILGF EE YN YKLTSVVMHMGNMTKDFVPVGKEEQAEIK E N KVAEICG+D EWMI YFCKPKLKVG EWV+KGQTCS A+SSV+GI RKIYELVFRFI DKCNETL DPTMKK+ YIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEW NVDFGMDLQKCI MFEKPMGLL+ILEEESLFPKATD +F+ KLHENLL KC NFQKA P P+P A FAV+HYAA VSYNLT WLEKNKDPLNDT+VELFKNGSN LL+E FRDHPGQPLE KKD G +KKGGGKTVSSFYKGQLDDLMK LYATDPSFIRCVVPNTHK+PG VESGLVMHQYQCNGVLAGIAICRKGFPNKM Y+DFK+RYNILAA VAKAK DK AAKAVL +KLE EK+RLGHTKVFFRAGILG+MEEVR+D++G VLSWLQ+QARGK+SRM+FKKMQDQKLALY CQRTIRN++IGKTWLWWQLWL +KPNLKCTKF+QYKAEYEEKIAIAE NI KA+AD KKV HE L+ +K+EL LAL+SGGSAVQDIIDK R+E ++QKQ+DE NNRI +E+ + SL QQ KV+ + +LR EI +E+ + E+DK+ KD QIRTLKEE+ HQ E+I+KL +EKRGV D++QKTEED+QA+ED+ +HL+KVK KLEQSLDE ED+LE EKK K D EK+KR+IE DLKLTQET+SDL+RVK EL+Q +QRKEKE +++ AKI+DE TLGGKY+KQ KELQ+RLEELDEEL IER +R KAEK R++L +D+EDLG +LE+AG NT+TQ+ELNKKRE EL ++KAE+EE NIGHEGTLAALR KHNNTM+E+GEQID+LN K K EKDK NMERDLQEAR+SL++ +R +A +++NGK+ QG I +AN KLDE+ARALNEA+S KK+L +E DL+RQI+E ENA+ K KISLTTQLEDTKRLADAEARDR++LL+K+KNL +ELES RERIE E E KSD LK LSKAQAE QLW+S++ETEG+G +EELEG + KL AR+ EAEET+++LN K+ S+EK++ RL+ +LE++ LEYER HAAA+ISEKRGRNFD+V+ EWKAK DDL+ E+EAS KE RNYNSELFRLRAA +E +EQLDVVKRENKNLADEI+DLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLR+QLELGQVRQEIDR+IQEKEEEF NTRKNH RAMDS+ ASLEAE RAKTEALRIKKKLE DINELEIALD ANKAN+E K++KRYQ Q R+ YE+ESR RQE+ E G+A RK NAL GE++E+R+LL S+ER KRQ + EL E R SVNEM++IN++A +KR ES IH + AEIDDM+ QAKNSEEKAK+AM+DAARLADELR+EQEHS ++++ KRAL+S +GELE RL +A AAK GRAA++KLE +IRELE+ELGS QSRTS++ KAYQ+AER +KELQFQQ+EDRKNQERM+ELA KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+RSK AE
Sbjct: 151 RRNECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQAGKLSGADMVTYLLEKSRLTFQAELERCYHSFYNLMSDAVPDLKEKCLLSNNIHDYWWVSQGKVTVPSIDDKEDMQFADEAFDILGFTTEEKYNSYKLTSVVMHMGNMTKDFVPVGKEEQAEIKDESNCKKVAEICGVDCEWMIMYFCKPKLKVGAEWVSKGQTCSGAASSVSGIGRKIYELVFRFIVDKCNETLVDPTMKKIMYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWKNVDFGMDLQKCITMFEKPMGLLSILEEESLFPKATDTTFSNKLHENLLGKCENFQKAKPXPEPXAXFAVIHYAAIVSYNLTGWLEKNKDPLNDTIVELFKNGSNRLLVEIFRDHPGQPLETKKDNSSGPKKKGGGKTVSSFYKGQLDDLMKVLYATDPSFIRCVVPNTHKQPGMVESGLVMHQYQCNGVLAGIAICRKGFPNKMVYNDFKSRYNILAATLVAKAKNDKAAAKAVLESIKLETEKYRLGHTKVFFRAGILGHMEEVRDDKIGLVLSWLQAQARGKSSRMIFKKMQDQKLALYCCQRTIRNWHIGKTWLWWQLWLKLKPNLKCTKFAQYKAEYEEKIAIAEANIDKAIADCKKVTKEHEKLMNEKNELVLALQSGGSAVQDIIDKTNRLESQKNELQKQVDETNNRIKSEEDSKASLNQQGSKVKQEADKLRNEIKDLESNMEKCEEDKITKDNQIRTLKEEICHQEELISKLQKEKRGVGDSRQKTEEDIQAMEDRCNHLNKVKAKLEQSLDECEDSLEREKKSKGDVEKLKRRIEGDLKLTQETVSDLDRVKGELNQTIQRKEKELSSLQAKIEDEQTLGGKYTKQIKELQTRLEELDEELAIERQNRGKAEKXRAILSRDIEDLGEKLEDAGNNTSTQIELNKKRESELTKLKAELEESNIGHEGTLAALRQKHNNTMAEMGEQIDSLNKLKAKTEKDKANMERDLQEARASLDEAMRERANIEKNGKLTQGLIVEANQKLDELARALNEADSSKKKLFVENQDLQRQIDETENAINTLGKAKISLTTQLEDTKRLADAEARDRTALLSKFKNLNSELESLRERIEEESESKSDLLKGLSKAQAETQLWRSKYETEGLGXIEELEGNKSKLHARLAEAEETIDSLNQKVASTEKTKHRLEAELEDLQLEYERVHAAAVISEKRGRNFDKVVSEWKAKCDDLSSELEASQKESRNYNSELFRLRAAWDETVEQLDVVKRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRAQLELGQVRQEIDRRIQEKEEEFENTRKNHIRAMDSMQASLEAETRAKTEALRIKKKLESDINELEIALDHANKANSEAHKSIKRYQNQHREVETAYEEESRLRQEILEKAGLAXRKXNALQGELDEARSLLDSAERGKRQAEMELGECRTSVNEMTNINSKANADKRRLESTIHTMHAEIDDMLHQAKNSEEKAKKAMIDAARLADELRAEQEHSNTQEKTKRALDSSIGELEQRLLEANEXAAKGGRAALAKLEAKIRELELELGSCQSRTSDTYKAYQKAERRIKELQFQQDEDRKNQERMSELATKLQQKIKTYKKQIEEAEEIAALNLAKFRKAQQEMEETEERSKMAE 1915 HSP 2 Score: 546.584 bits (1407), Expect = 2.129e-161 Identity = 237/263 (90.11%), Postives = 251/263 (95.44%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGH+KK+ GPDPDP+PWL V+PELK KLKSKPYD KKSCWVPDKA+GGY EGLI+STDGDKVTV IL + DKKVFKKDQVGQVNPPKFDC+DDM+GLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQR++D+Y GKRR+ECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQ GKLSGADM
Sbjct: 1 MPGHVKKTTGPDPDPTPWLEVTPELKEKLKSKPYDAKKSCWVPDKATGGYLEGLIESTDGDKVTVKILSSNDKKVFKKDQVGQVNPPKFDCADDMAGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRTMDIYSGKRRNECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQAGKLSGADM 263 HSP 3 Score: 65.4698 bits (158), Expect = 1.899e-10 Identity = 211/977 (21.60%), Postives = 395/977 (40.43%), Query Frame = 0
Query: 1153 EHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEI------------EERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLA---HEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVK-------RENKNLAD-EIRD---LLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLE---LGQVRQEIDRKIQEKEEEFNNTRKNHQR------AMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQK-------AVKRYQGQLRDT---IQGYEDESR------FR-----QEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDA-----ELEESRGSVNEMSSINTRAMHEKRATESGIH--AIQAEIDDMMTQAKNSEEKAKRAMVDAA-RLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEM---------------------ELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQK-------LQQKIKTYKQQIEE-------AEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
E+E+K+ + + I K+T+E E++ E ++ V + +A+ ID N L Q ELQ +++E + + E S+A + S +K++ + L + +++ +N E ++ ++ +K EI E+R +G R K + + ++ ++LN K K +E+ L E SLE ++K ++++ + I+G + + ++ R E +R E E L+ +IE+ + ++ KQ L T+LE+ E ++R L+ ++E E++E+ S ++ K ++E+ K+ E +G L R K + E E +++LN +EK ++ ++ DL+E + EK G+ +I E K D+LA +E ++S K+ N +L R E AI L K + K LAD E RD LL + + + L ++ K +L L +A+A E ++ RS+ E LG + EE N K H R +DSL+ + A TE + K +LE ++ +L++ ++ + A +K V ++ + D ++ + ESR FR E E + + R+ LA E+++ L RS +LD E+E+ + E + + + ++ EID + + + E ++ + A + L +E T R K+ LES + ELE L D A ++ + + + RE+E + ++Q E+ AER ++ + + E R + MT + K L+ I T +I++ +EE A + + E ++ S T EK+
Sbjct: 852 EYEEKIAIAEANIDKAIADCKKVTKEH----EKLMNEKNELVLALQSGGSAVQDIIDKTNRL----ESQKNELQKQVDETNNRIKSEEDSKASLNQQGSKVKQEADKLRNEIKDLESNMEKCEEDKITKDNQIRTLKEEICHQEELISKLQKEKRGVGDS------RQKTEEDIQAMEDRCNHLN-------KVKAKLEQSLDECEDSLEREKKSKGDVEKLKRRIEGDLKLTQETVSDLDRVKGELNQTIQRKEKELSSLQAKIEDEQTLGGKYTKQIKELQTRLEELDEELAIERQNRGKAEKXRAILSRDIEDLGEKLEDAGNNTSTQIELNKKRESELTKLKAELEESNIGHEGTLAALRQKHNNTMAEMGEQIDSLNKLKAKTEKDKANMERDLQEARASLDEAMRERANIEKNGKLTQGLIVEANQKLDELARALNEADSSKKKLFVENQDLQRQIDETENAINTLGKAKISLTTQLEDTKRLADAEARDRTALLSKFKNLNSELESLRERIEEESESKSDLLKGLSKAQA-----ETQLWRSKYETEGLGXI-----------EELEGNKSKLHARLAEAEETIDSLNQKV-----ASTE--KTKHRLEAELEDLQLEYERVHAAAVISEKRGRNFDKVVSEWKAKCDDLSSELEASQKESRNYNSELFRLRAAWDETVEQLDVVKRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRAQLELGQVRQEIDRRIQEKEEEFENTRKNHIRAMDSMQASLEAETRAKTEALRIKKKLESDINELEIAL-DHANKANSEAHKSIKRYQNQHREVETAYEEESRLRQEILEKAGLAXRKXNALQGELDEARSLLDSAERGKRQAEMELGECRTSVNEMTNINSKANADKRRLESTIHTMHAEIDDMLHQAKNSEEKAKKAMIDAARLADELRAEQEHSNTQEKT 1783
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000006593 (supercontig:LSalAtl2s:LSalAtl2s363:281296:303912:1 gene:EMLSAG00000006593 transcript:EMLSAT00000006593 description:"snap_masked-LSalAtl2s363-processed-gene-3.4") HSP 1 Score: 1888.62 bits (4891), Expect = 0.000e+0 Identity = 971/1770 (54.86%), Postives = 1293/1770 (73.05%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDN--AVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPK-PDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFD--RVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+E PPHIF +A+G YQ M+ KNQSILITGESGAGKTENTKKVI YFA VGA+GK +G+ASLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI FNQ GKLSGADM +YLLEKSR+ F LER YH FYNLMSDA+P+LK+ CLLS+NI DY +VSQGKV+VESIDDKEDMQ+A +AF ILGF++EE NVY+ T+ VMHMG M F ++ + E+N A VA + GID + + + +PK+KVG+EWVTKGQ SQA ++VAGIAR I+E FR + KCNETL DP+M+++ +IG LDIAGFEIFDYNGFEQ+CINFCNEKLQQFFN HMFVLEQEEYVREGIEWA VDFGMDLQKCIDMFEKPMG+L+ILEEESLFPKATD++F KL N + K P FQK P PD AHFAVVHYA TVSYNLT+WLEKNKDPLNDTV++ KNGSN L++E FR HPGQ + D+ G +KKGGGKTVSSFYK QL LM TL+AT+P FIRC+VPNTHK+ G +++GLVMHQ CNGVL GI ICRKGFPN+M Y++FK R AG+LG MEE+R+D+V E+LSWLQS ARG SR+ F+KMQ QK+ALY QR+IRN+ IGKTWLWWQLWL IKPNL+ +KF++ KA E K AE I DR++ E+ +E L + EL L G S V+D+ K R+E ++ KQ++EV R+ E++ +++ K+ S +L+++ +M+ ++ E+D+ KD QIR+LKEEL HQ +++ KL +EK+ +N+QKTEED+Q ED+++HL+++K KLEQ+LDE ED++E EKK K D EK +RKIE DL+ +Q+++++L+R K E++ AVQ KEKE +A+ KI+DE +LG K KQ +EL SRL EL+EEL +ER R K+EK R +L ++L DLG +LEE+G T+TQ+ELN+KRE EL ++K E++ + HE LA+LR KHN +S+LG+QID +N K K E+ K + ++ + R+ +ED + K+ +DR KM+ I D + ++D++ +L+E++ +K+L +EK DLE+Q E+ EN + K K SL TQLED +RLA AEAR+R++LL K++NL ++LE+ RERIENE+E K + K +S+A AE Q+WK++F TE + R+E+L+ + K+ ARI EAEE ++ L NK+ ++EK ++R Q+DLE++ +E ER A+ + +F +V+ EW+ K DDL+ E++AS K+CR+++SELFRLRAA +E +EQLD VKRENKNLADEI+DLLDQLG+GGRSIHELDKQRRRL++EKEELQ+ALEEAE+ALEQEEN+V+R Q+E+ Q +QEI+R++ EKEEEF+NTRKN+QRA+DS+ ASL+ E +AK EALRIK+KLE DINE+E+ALD +NKANAE K +KR+ L + E+E R ++Q+ +G ++RKGN LA E++ES+ LL ++ER+KR + E+ ESR ++NE+++ N+ ++KR ES + A+Q E+DD + Q KNSEEKA++A+ DA +Q EL+ RL E+ A K GR +SKLE R+R LE E G QS+TSE+ K + R +R++KE+QF +E++KN E+M EL KLQ KI+ YK+QIE+AEEIAALNLAK+RK+QQ+ EE+E+RS+ AE
Sbjct: 150 RRNEIPPHIFAIADGGYQSMLTHQKNQSILITGESGAGKTENTKKVIGYFACVGATGKSLDGKASLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFNQAGKLSGADMEVYLLEKSRIXFXQPLERSYHIFYNLMSDAIPNLKKMCLLSNNIKDYHYVSQGKVNVESIDDKEDMQFADEAFDILGFSKEEKQNVYRCTATVMHMGEMK--FKQRSSKDDQAVSEENNPTAYNVASLLGIDEDILCDNLVQPKIKVGSEWVTKGQNVSQAYNAVAGIARAIFEKQFRHLVAKCNETLVDPSMRRITFIGVLDIAGFEIFDYNGFEQLCINFCNEKLQQFFNHHMFVLEQEEYVREGIEWAMVDFGMDLQKCIDMFEKPMGVLSILEEESLFPKATDKTFEEKLFANHMGKSPTFQKPKPGGPDKDAHFAVVHYAGTVSYNLTNWLEKNKDPLNDTVIDQIKNGSNKLIVEVFRSHPGQSGDDSGDSRSGKKKKGGGKTVSSFYKEQLIHLMTTLHATEPHFIRCIVPNTHKQAGVIDAGLVMHQLTCNGVLEGIRICRKGFPNRMIYEEFKNR-------------------------------------------AGVLGTMEELRDDKVSEILSWLQSTARGSMSRVTFRKMQAQKMALYCVQRSIRNFMIGKTWLWWQLWLAIKPNLRSSKFAEIKATLEAKTKEAESQISGVKKDRQQAESANETLRSETQELEDNLSKGNSLVRDMEMKVKRIEAQKKELDKQVNEVAKRLQEEEETCNAINNVIRKLDSDSKRLKDDSRSMDAKIQQCEEDRETKDSQIRSLKEELIHQEDLVNKLMKEKKMSAENRQKTEEDLQIAEDRTNHLNRLKVKLEQNLDEIEDSVEREKKAKLDIEKTRRKIEGDLRCSQDSVAELDRAKNEINHAVQMKEKELSALAXKIEDEQSLGNKLQKQMQELSSRLSELEEELEVERSLRTKSEKGRQILSRELADLGEKLEESGNATSTQIELNRKRELELAKLKEELDNSALQHESALASLRQKHNGIISDLGDQIDQVNKGKAKVEQQKNVLIMEMNDTRALVEDLSQEKSNIDRQNKMLNAEISDGSQRVDDLQNSLSESDMSRKKLNLEKSDLEKQTEDAENQLQMLKKLKTSLNTQLEDIRRLAXAEARERATLLGKFRNLESDLENIRERIENENEAKGEIQKQMSRALAETQVWKTKFTTEAVARIEDLDNAKSKIIARIEEAEECIDGLQNKVATTEKLKTRYQMDLEDLQVECERFKDIALKNIILYCHFHLCKVVNEWRMKCDDLSTELDASQKDCRSHSSELFRLRAAWDETVEQLDTVKRENKNLADEIKDLLDQLGEGGRSIHELDKQRRRLQIEKEELQSALEEAESALEQEENRVIRLQMEVAQAKQEIERRLSEKEEEFDNTRKNYQRAIDSMQASLDGEIKAKQEALRIKRKLEEDINEMEMALDHSNKANAEAMKQIKRHAAHLLEVETCVEEECRAIADIQDQIGXSERKGNVLAAELDESKMLLETAERAKRSAELEVSESRDAINELTNTNSIMGNQKRKKESELKALQQELDDFIIQVKNSEEKARKAITDAG------------------------AQCSELQNRLDCVESAALKHGRKIISKLEERLRSLENEYGFTQSKTSETHKNFIRTDRNIKEMQFNMDENKKNIEKMGELLDKLQGKIRVYKKQIEDAEEIAALNLAKYRKAQQQLEEAEERSQQAE 1850 HSP 2 Score: 402.905 bits (1034), Expect = 4.684e-114 Identity = 185/265 (69.81%), Postives = 219/265 (82.64%), Query Frame = 0
Query: 1 MPGHIKK--SDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPG++K+ + PDPDP+P+L +S E K K +K YDPK+SCWVP K GG+ +G+I+ST+GDKVTV + E DK++ KKDQV QVNPPKF+ +DMS LTYLNDA VL N RY ++LIYTYSGLFC+AINPYKRFPIYT+ +I LYI KRR+E PPHIF +A+G YQ M+ KNQSILITGESGAGKTENTKKVI YFA VGA+GK +G+ASLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI FNQ GKLSGADM
Sbjct: 1 MPGNVKRGGTGEPDPDPTPFLFISYEEKQKDLAKAYDPKRSCWVPIK-EGGFDQGVIESTEGDKVTVKVGE--DKRIIKKDQVQQVNPPKFERCEDMSNLTYLNDASVLHNLKARYLSKLIYTYSGLFCVAINPYKRFPIYTETAIKLYINKRRNEIPPHIFAIADGGYQSMLTHQKNQSILITGESGAGKTENTKKVIGYFACVGATGKSLDGKASLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFNQAGKLSGADM 262
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000006889 (supercontig:LSalAtl2s:LSalAtl2s38:10457:140541:1 gene:EMLSAG00000006889 transcript:EMLSAT00000006889 description:"maker-LSalAtl2s38-augustus-gene-1.16") HSP 1 Score: 1761.12 bits (4560), Expect = 0.000e+0 Identity = 935/1805 (51.80%), Postives = 1262/1805 (69.92%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASG---KRKEG--EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEI---CGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKG---------------GGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKA-----------KKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKSPIRI 2034
RR+E PPHIFG+AEGSY M KNQSILITGESGAGKTENTKKVI+YFA VG++ K K G + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIR+WFN GK++G D+ YLLEKSR+T+Q+ +ER YH FY +++ V DL++ C+LS++I++Y +S GKV VESIDDKE+M +AF ILGF +EE Y+VYK++S+ MH+ + + G E K+ D + + C E + + FC PK+KVG EWV K Q + V I + I+ +FR++ D CN TL DPTMKKV YIG LDIAGFEIFD+N EQ+ INF NEKLQQFFN +MFVLEQEEY+REGIEW +VDFGMDL CID+FEKPMG+L ILEEE+++PKA+D++F KL L K NF +A K D AHFA++HYA TVSYN+T WL+KN+DP+NDT HPGQ E + DA G ++G KTV S++K QL++L+ L +T+PSFIRC+VPN K PG V+ LVMHQ CNGVL GI IC KGFPN++ Y DF+ RY ILA KA KK+ A AV++ L +F+ GHTK+FFRAG+LG MEE+R+DRV ++++LQS RG ++ V+KK+ + K L QRTIRNY +G+ W WWQ+WL +KPNLK +F +K E E+I A++++ + R+ E+ ++ L E+ L++ G +A QDI+DK R E + D K++ + R+ +E + +SL+Q K++S QS L E+ E +L + + K +K+ QI+ +K+E+ HQ E++ KL REK+ V + +QK EE +Q++ED+S+HLSK+K +LE+ LDE ED E EKK +ND EK KR IE +LKLTQE +SDLER+ EL+Q VQRKEKE ++N KI+DE TLG K + Q KEL RLEELDE+L ER +RA+A+K++ LK++LE+L +LEE G+NT Q+ LN +RE+EL ++K+E+EE NI HE TLA LR KHN++++++ + +D LN K EK++ N+ ++L+ L+ K L+++GK+IQ S + + +L+E RAL+EA+ K++L +E DL +EEGE A NK K S TTQLED KRLADAE R+R +LL K +NL EL +E ++ E + K + + LSKA A++QLWK+RFETEG+ R+EE+E R K+ +R+ EAE+T+ AL KI EKS+SR+ + E+++ E +R + A I EKRGRNFD+VI EWK KA+DL+ EI AS ECRN++SE FR++++++E E LD VKRENKNLADEI+DL+DQLG+GGRSIH+LDK RR+LE+EKEELQAALEEAEA LEQEENKVLR+QLE+ QVRQEIDR+I+EKEEEF++ +KNH R MDS+ ASLEAE RAK E LRIKKKLE DINE+EIALD ANKA+ E +KA KR Q QL D E+E + EV+E +G+A+RK NAL GE+EES+ALL +S+R + Q++ EL +S+ +++ N ++ KR ES IH IQA+ID+++ Q+K +EEKAKRAMVDA RLADELR+EQ+H+ ++D+ R + L +L + +A A+A+ +KLE RIR++E EL T+E K + ER VKEL FQ +E++KNQ+RM +L KLQQKI +YK+QIEEAEEIAA+NLAK+RK QQ+ EE+E+R+K A + RI
Sbjct: 3016 RRNEVPPHIFGIAEGSYHSMCMKNKNQSILITGESGAGKTENTKKVITYFAFVGSTAGSKKDKSGAKKVSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRVWFNNQGKMAGGDIETYLLEKSRVTYQSPMERSYHIFYFMITHQV-DLRESCMLSEDIFEYPLMSMGKVFVESIDDKEEMSIMDEAFDILGFTQEEKYDVYKVSSLCMHLSKLEFN----GHGEITTAKNLDAGTDILSLFNWCDSADE-LYDRFCNPKIKVGMEWVNKSQNLTNVMVGVGSIIKNIFGRLFRYLVDMCNNTLIDPTMKKVNYIGVLDIAGFEIFDFNTLEQLMINFVNEKLQQFFNHNMFVLEQEEYMREGIEWVSVDFGMDLAACIDLFEKPMGILPILEEETIYPKASDKTFEEKLKSTHLGKHNNFARACSKTDKDAHFAIIHYAGTVSYNVTGWLDKNRDPINDTX------------------HPGQTKE-EDDAPTPGHRRGKKRXAVKSKTAAKMANFKTVCSYFKDQLNNLINMLMSTEPSFIRCIVPNGIKTPGLVDPFLVMHQLTCNGVLEGIRICTKGFPNRVMYADFRQRYAILAPKEAHKAMKMVKRPVTEDKKNIAATHAVMDKTPLTLAQFQYGHTKIFFRAGVLGQMEEMRDDRVNGLITFLQSWMRGXHTKKVYKKLWEHKRGLLCIQRTIRNYMMGQKWQWWQIWLALKPNLKSGRFEDFKKELAERIIYAQEHLDEVKRQREVSESKNKSLTKDLDEIRLSISGGTNAKQDILDKIARAEEITGDYHKEILAIKQRVTSEHESCESLSQSLKKIESNQSGLTRELKEYEMKLNSVQNQKADKEMQIKQMKDEISHQEEIVNKLNREKKNVIEARQKEEEKIQSIEDRSNHLSKLKFRLEKQLDEIEDTWEREKKHRNDIEKQKRVIEGNLKLTQEAVSDLERINLELNQVVQRKEKELGSLNGKIEDEQTLGSKINLQIKELNIRLEELDEDLEAERLARARADKAKCNLKRELEELHEKLEETGSNTTAQIALNTRREEELSKLKSELEESNISHESTLAVLRQKHNSSITDMADNMDQLNKQKAMVEKERNNIMQELESITLQLQAEQSEKTSLEKSGKLIQNSTNELSVRLEEKQRALHEADGTKRKLMIENCDLVHHLEEGERLCASLNKDKTSFTTQLEDAKRLADAETRERINLLGKMRNLEHELIIIKEHLDQEFDSKQEVERQLSKAFADVQLWKTRFETEGVARIEEIERDRSKISSRLREAEDTISALQEKIAVLEKSKSRMSTESEDLTSECDRQNTNASIIEKRGRNFDKVINEWKCKAEDLSSEITASQSECRNFSSEYFRIKSSNDEIQEHLDTVKRENKNLADEIKDLMDQLGEGGRSIHDLDKTRRKLEIEKEELQAALEEAEATLEQEENKVLRTQLEMSQVRQEIDRRIREKEEEFDHHKKNHLRCMDSMQASLEAETRAKEEILRIKKKLESDINEMEIALDHANKAHNEARKATKRTQAQLADINSAIEEERKICNEVEEQLGLAERKSNALFGELEESKALLEASKRGQNQVEQELCDSKEQTSDLQMSNQALLNNKRKLESDIHQIQADIDNLLCQSKAAEEKAKRAMVDAGRLADELRAEQDHTCTQDKIIRVTDKNLSDLALKAEEASAHASNAAINVPAKLESRIRDIETELNKTILMTAECHKYVTKGERRVKELGFQTDENKKNQDRMADLVDKLQQKIHSYKKQIEEAEEIAAINLAKYRKXQQDMEEAEERTKIASEQVHRI 4795 HSP 2 Score: 366.311 bits (939), Expect = 7.853e-102 Identity = 177/270 (65.56%), Postives = 208/270 (77.04%), Query Frame = 0
Query: 1 MPGH--IKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASG---KRKEG--EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPG + K+ DPDPSP+L VS E + K KPYDPK+SCWVPD + + EGLI T G KV V + + K+ FK+DQV QVNPPKFD DDMS LTYLNDA VL+N RY +LIYTYSGLFCIA+NPYKRFPIYT+R++D+Y KRR+E PPHIFG+AEGSY M KNQSILITGESGAGKTENTKKVI+YFA VG++ K K G + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIR+WFN GK++G D+
Sbjct: 2865 MPGTRIVLKAGEEDPDPSPYLFVSNEQRIKNSEKPYDPKRSCWVPD-SEEKFLEGLIQETTGGKVKVQLNKNKEVIEFKQDQVAQVNPPKFDMCDDMSNLTYLNDASVLFNLKARYVEKLIYTYSGLFCIAVNPYKRFPIYTRRTVDIYRMKRRNEVPPHIFGIAEGSYHSMCMKNKNQSILITGESGAGKTENTKKVITYFAFVGSTAGSKKDKSGAKKVSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRVWFNNQGKMAGGDI 3133
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000003084 (supercontig:LSalAtl2s:LSalAtl2s173:1520085:1527818:1 gene:EMLSAG00000003084 transcript:EMLSAT00000003084 description:"maker-LSalAtl2s173-augustus-gene-15.13") HSP 1 Score: 1617.05 bits (4186), Expect = 0.000e+0 Identity = 856/1774 (48.25%), Postives = 1213/1774 (68.38%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE-ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKSPIRIPV 2036
RR+E PPH++ + E Y+ M+ KNQS+LITGESGAGKTENTKKVISY A V +SGK+ + SLED+IV TNP+LE++GNAKT RNDNSSRFGKFIRI F GKL+G D+ YLLEKSR+T Q E+ER YH FY L+ VPD+K KC L D+IYDY +VSQGKV+V SIDD E+++Y AF I+GF E+ET+ ++LTS VM+MG + F G+++QAE K+ E+ G++++ ++ FCKPK+KVGTEWVTKGQTC+QA++ V GIAR I++ +F+++ KCN+TL D +MKK ++ LDIAGFEIF+YNGFEQI INF NEKLQQFFN HMFV+EQEEYV EGI+WA VDFGMDL I MFEKPMG+ AILEEESLFPKATD+SF KL L K F K K D +AHFA++HYA VSYN+T WLEKNKDP+NDTVV++ K SN LL+ +R+HPGQ D G +K GGGKTVSS Y QL +LM TL++T+P FIRC+VPNTHKKP VE L+MHQ CNGVL GI IC +GFPN+M Y ++K+RY IL A+ +A A +K A++N + + EK+RLGHTKVFFRAG L +EE R++ V +++ W+Q + G R +KK DQ+ L QR R Y + + W W+ + +P + + E K A +AL K +E + + +K +LT L+S + D+ T+ + L +A +Q R +T + K++ Q ++++I +E + EQ+K +D + TL EE+ Q E+I KL +EK+ + DN+ K ED+ ++K SHL+ +K KLE +LDE E +++ EK+ + + EK +RK+E +LK+ QE++SDLE K EL + RKEK+ +++N K++DE ++ K K KE Q R+EEL+EEL ER +RAKAE+ RS L ++LE+LG RL+EAG T+ Q+ELNKKRE E+++++ +IEE NI E L+ L+ KH + + E+ EQID LN K K EKDK ++ ++ +AR++ ++ RAKA +++ K + + D N K++E L + ES K++L E DL R + N + K K SL + LED K AD EAR+R LL K+KNL E++ RE +E E + D + ++K + E LW++++E+E + + EELE ++ KLQAR+ EAE T+E LN K+ EKS+ +L +++EEMS+ ++ EK+ R FD+++ EWK K D L+ +++ S KECRN +SELFR+++A+EE++ QLD V+RENK L++EI+D++DQ+ +GGRSIHE+DK R+RLE EK ELQAALEEAE ALEQEENKVLR+QLEL QVRQEI+R+I EKEEEF +KN +A++ + A+LE E + K EALR+KKKLE D+ ELE++L+ +N N E QK++K+YQ Q+R+ EDE R ++ ++A+ +++RK +A+ +EE+R LL ++R++R + EL + S+++ + N KR ES + +QA++D+M +A+ +EKA ++M+DAARLADELRSEQ+H+ S ++ ++ LE+Q +++TRL +AE+NA K G+ AM+K+E RIRELE E+ + R ++S K +++ER +KEL F +EDRKN ERM L +LQ +IK+YK+QIEEAEEIAALNLAKFRK+Q ++E R+ +E++ + V
Sbjct: 150 RRNEVPPHLWAITEXXYRNMLQNLKNQSMLITGESGAGKTENTKKVISYLAMVASSGKKTSTKKVSLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFTSSGKLAGCDIESYLLEKSRITQQQEVERSYHIFYQLLQPFVPDMKSKCCLGDDIYDYSYVSQGKVTVASIDDNEELEYTFSAFDIIGFGEQETWECFQLTSAVMNMGEV--HFKQKGRDDQAEPDDMTYPNKIGELMGVNADELMKSFCKPKIKVGTEWVTKGQTCNQATNGVGGIARAIFDRLFKWLILKCNDTLIDRSMKKSNFVAVLDIAGFEIFEYNGFEQISINFVNEKLQQFFNHHMFVVEQEEYVTEGIDWAMVDFGMDLAAAIIMFEKPMGIWAILEEESLFPKATDKSFEEKLKAQHLGKSXPFAKPQSKTDKNAHFAIIHYAGIVSYNVTGWLEKNKDPVNDTVVDVLKRASNELLVFLWREHPGQS-NPPDDKGKKKKKGGGGKTVSSVYLVQLSELMHTLHSTEPHFIRCIVPNTHKKPIEVEPPLIMHQLTCNGVLEGIRICMRGFPNRMLYPEYKSRYQILGASEIATASDNKTGVTALMNKISFDCEKYRLGHTKVFFRAGALAALEEARDEIVLKLVRWMQGEIFGNVKRKEYKKKFDQRELLKVIQRNFRKYQVLRNWGWFIMIQKTRPLIGQVNIEEELRLLETKANDAFGAYQEALNVTKDLEXANIQIDEEKKQLTKQLESEQGNLSQYQDRQTKALKLKVSADSDLVVAQQNLAKAEQDRIEMTADRKKLEGQVGLVKKDIEDLELAIQKVEQEKTNRDHILHTLNEEVAEQDEVINKLNKEKKHIADNQSKASEDLVQADEKVSHLNSIKAKLESTLDELESSVDREKRTRAEIEKSRRKVEGELKICQESVSDLEHSKKELENCILRKEKDASSLNHKLEDEQSIVSKLQKSIKENQGRVEELEEELEAERQARAKAERQRSDLARELEELGERLDEAGGATSAQMELNKKRENEVNKLRRDIEEANIQQESILSNLKKKHQDAIQEMTEQIDQLNKMKSKIEKDKTKIQHEIADARAATDEICRAKASSEKSNKNLICQLNDVNKKVEEANMTLGDFESHKRKLAAENGDLLRIAGDINNNVNMLQKMKFSLQSALEDAKHNADDEARERCLLLGKFKNLEHEVDGLRENLEEEISARDDLNRQINKCEGEANLWRTKYESEAVAKAEELEMSKMKLQARLTEAESTIENLNCKLSQVEKSKGKLHLEIEEMSISLDQAQILNNQMEKKARQFDKIVQEWKGKVDGLSMDLDVSQKECRNASSELFRVKSAYEESVSQLDEVRRENKTLSNEIKDIMDQISEGGRSIHEIDKIRKRLEAEKIELQAALEEAEGALEQEENKVLRAQLELTQVRQEIERRIAEKEEEFQGIKKNQGKAVEGMQAALEQESKGKAEALRMKKKLESDVGELEMSLEHSNANNLETQKSIKKYQQQIRENQSKLEDEQRAKENARDALVVSERKSHAMQNALEEARTLLEQADRNRRINEQELSDVNESLSDATVQNQAIAAAKRKLESEMQTLQADLDEMAVEARLCDEKASKSMIDAARLADELRSEQDHAQSLEKSRKLLEAQAKDMQTRLDEAESNALKGGKKAMNKMETRIRELESEMDAENRRLADSQKNLRKSERRIKELTFAGDEDRKNHERMQSLIDQLQGRIKSYKKQIEEAEEIAALNLAKFRKTQAVLGDAESRADISEQALAKAKV 1920 HSP 2 Score: 362.073 bits (928), Expect = 3.599e-101 Identity = 163/266 (61.28%), Postives = 203/266 (76.32%), Query Frame = 0
Query: 1 MPGHIK--KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG-EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGH+K K PDPDP+P+L+VS E+K + KPYDPKKS WVPD GGY GL++S+ GDK TV + +KK FK ++VGQVNPPKF+ +DM+ LTYLNDA V N R++ +LIYTYSGLFCI +NPYKR+PIYT R + +Y+GKRR+E PPH++ + E Y+ M+ KNQS+LITGESGAGKTENTKKVISY A V +SGK+ + SLED+IV TNP+LE++GNAKT RNDNSSRFGKFIRI F GKL+G D+
Sbjct: 1 MPGHVKLGKPGEPDPDPAPFLVVSMEMKREDMLKPYDPKKSVWVPD-GKGGYIAGLLESSSGDKTTVAL--GHEKKTFKSEEVGQVNPPKFEKCEDMANLTYLNDASVFHNLKTRFQAKLIYTYSGLFCIVVNPYKRYPIYTARVVKMYLGKRRNEVPPHLWAITEXXYRNMLQNLKNQSMLITGESGAGKTENTKKVISYLAMVASSGKKTSTKKVSLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFTSSGKLAGCDI 263
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000011566 (supercontig:LSalAtl2s:LSalAtl2s800:119109:125711:-1 gene:EMLSAG00000011566 transcript:EMLSAT00000011566 description:"augustus_masked-LSalAtl2s800-processed-gene-1.7") HSP 1 Score: 1574.68 bits (4076), Expect = 0.000e+0 Identity = 826/1767 (46.75%), Postives = 1198/1767 (67.80%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RR+E PPH++ + E +Y+ M+ K+QS+LITGESGAGKTENTKKVISY A V +SGK++ + +LED+IV TNP+LE++GNAKT RNDNSSRFGKFIRI FN GKLSG D+ YLLEKSR+T Q E+ER YH FY L+ VP++ +KC L+D+IYDY +VSQGKV VESIDD E+M++ +AF +LGF EEE +N YK+T VM G ++ F G+++QAE K++ + G+ + M+ F KPK+KVGTEWVTKGQ QA+++V GIAR Y+ +F ++ + CN+TL D ++KK ++ LDIAGFEIF++NGFEQI INF NEKLQQFFN HMFV+EQEEY+REGI+W VDFGMDL CI MFEKPMG+ AILEEESLFPKATD+SF KL L K F K K D +AHFA++HYA VSYN+T+WLEKNKDPLNDTVV++ K+ +N LL+ +RDHPGQ ++ G +K G KTVSS Y QL+DLM TL++T+P FIRC+VPNTHK+ G +E LVMHQ CNGVL GI IC +GFPN++TY D+K+RY IL A + KA K ++ +K + K++LGHTKVFFRAG L +EE+R+ V ++ WLQ++ G +R + K +Q+ L QR R + + W W+ + +P + + E K A + L +KK+E E + + + L + + +K ++ AD++ QL+E + + +Q+R TQ + ++++ S ++ EI+ + + ++ EQ+K +D QIR L +++ +Q E+I KL ++KR + DNK K E++Q +DK HL+ +K KLEQ+ E +D+LE EK+ + + EK +RK+E +LK+TQ T+ +LER K EL ++ R+E E + +K+DDE KY++ KE+Q R+EE++EEL ER +RAKAE+ RS L ++LE++G RLEEAG T+ Q+ELNKKRE E+ +++ ++EE +I E T+ L+ +H + + E+ EQI+ L+ K K +KDK + +++ + S L++ R+KA +++ K + + D ++DE +++ E+ K++L E D RQ++E E+ K K+ L QLE+ K D EA++RSSLL K++NL E++ R+ E E KS+ + KAQ+E+ W+ +FETE + + EELE ++ KLQAR+ EA+ T+E LN K +KS+ +L DLEE + ++ EK+ ++FD+++ EWK KAD+ + +++ S KECRN +SELFR+++A EE + QLD V++ENKNL+ EI+D++DQ+ +GGRSIHE+DK RRRLEVEK+EL +ALEEAE ALEQEENKVLR+Q+EL QVRQEI+R++QEKEEEF +KN +A++ L SLEAE + K EA R+KKKLE DI ELE+AL+ AN AN E Q+ +K+YQ +R+ +EDE + + +E A+R+ NA +EE++ LL S+R +RQ + EL ++ +++++ N KR E I ++ E+DDM ++A+ S+EKA R+M+DAA++ADELR+EQ+ S ++ ++ LES + + +L +AE NA K G+ AM K+E RIRELE EL + Q R +S K ++++ER +KEL + +EDRKN ERM L +LQ K+++YK+QIEEAEEIAA+NLAKF+++Q ES +R+ E++
Sbjct: 150 RRNEVPPHLWAITETAYRNMLTNTKDQSMLITGESGAGKTENTKKVISYLAMVASSGKKQTRKTTLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFNSSGKLSGCDIESYLLEKSRITQQQEVERSYHIFYQLLMPTVPNMIEKCNLTDDIYDYSFVSQGKVKVESIDDNEEMEFTDNAFDVLGFTEEEKWNCYKITXAVMSFGEVS--FKQKGRDDQAECDELIYPNKISNLFGVSCDQMMKAFIKPKIKVGTEWVTKGQNVEQATNAVGGIARACYQRLFNWLIEMCNKTLIDSSLKKTNFVAVLDIAGFEIFEFNGFEQISINFVNEKLQQFFNHHMFVVEQEEYIREGIDWVMVDFGMDLAACILMFEKPMGIWAILEEESLFPKATDKSFEEKLKATHLGKSSTFAKPQSKTDKNAHFAIIHYAGIVSYNVTNWLEKNKDPLNDTVVDVLKHANNALLVHLWRDHPGQTYPPEETKGKKKKKGGSTKTVSSVYLVQLNDLMNTLHSTEPHFIRCIVPNTHKQAGMIEQPLVMHQLTCNGVLEGIRICMRGFPNRITYKDYKSRYFILGANELKKASDQKTGVLNLMENIKFDKTKYKLGHTKVFFRAGALALLEEMRDTIVVRLIRWLQARFYGSLARKKYDKKAEQRKLLIVIQRNFRKFMQLRNWGWFIIIQKTRPLIGQINIEEELKALESKAKEAYGAYQEQLDTKKKLEAESEKIKDETKTMLQQLSKEQGNLSEFHEKQAKISAQKADLEVQLNEAIDLLNKHEQERLQATQNKKSLEAENSGIKREIDDLHSVVSKLEQEKTSRDHQIRMLNDDITNQDEIINKLNKDKRYLSDNKSKANEELQTAQDKVEHLNMIKVKLEQTRYELDDSLEREKRSRAEIEKSRRKVEGELKITQGTVLELERQKKELENSIARRESEINHLASKLDDEQVGVTKYTRNIKEVQCRVEEMEEELEAERQARAKAERQRSDLARELEEMGERLEEAGGATSAQIELNKKRESEVSKLRKDLEECHIQQEATMMNLKKRHQDAVFEMTEQIEQLSKMKSKIDKDKITITQEISDVNSGLDEITRSKASAEKSNKQLLDQLNDITKRVDESKLTIHDYENSKRKLLSENSDHLRQLQELESNYQMLAKIKVQLAHQLEEAKMYGDDEAKERSSLLAKFRNLEHEIDGMRDHYEEEKANKSELNRQYCKAQSELDSWRCKFETEALAKAEELEMSKMKLQARLAEAQGTIENLNGKYSMLDKSKIKLSTDLEEAIINLDQAQVLNSQMEKKAKSFDKIVKEWKTKADNFSMDLDNSQKECRNSSSELFRVKSAFEECVLQLDEVRKENKNLSSEIKDIMDQISEGGRSIHEIDKIRRRLEVEKQELTSALEEAECALEQEENKVLRTQIELTQVRQEIERRLQEKEEEFGAIKKNFGKAIEQLQTSLEAESKYKAEAFRMKKKLEADITELEVALEHANAANMESQRTIKKYQNMIREAQMKFEDEQSAKNDAKEVQLEAERRSNASQNALEEAKTLLEQSDRHRRQFEQELCDTNEVLSDLTVQNQSLEASKRKFEGEISSLSGEMDDMQSEARMSDEKATRSMMDAAKIADELRNEQDISIRLEKDRKYLESVAKDFQNKLDEAEQNALKSGKKAMGKMESRIRELESELDTEQRRLGDSMKNFRKSERKIKELTYSCDEDRKNHERMQVLVDQLQSKVRSYKKQIEEAEEIAAINLAKFKQTQNNLNESFERADINEQA 1914 HSP 2 Score: 372.089 bits (954), Expect = 2.563e-104 Identity = 166/265 (62.64%), Postives = 205/265 (77.36%), Query Frame = 0
Query: 1 MPGHIK--KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGH+K KS+ PDPDP P+L+VS E++ KPYD KKS WVPD GGY E L+DS +G K TV I K KV+K D+V QVNPPKF+ DDM+ LT+LNDA VLWN RY ++LIYTYSGLFC+ +NPYKRFPIYTQ + +Y+GKRR+E PPH++ + E +Y+ M+ K+QS+LITGESGAGKTENTKKVISY A V +SGK++ + +LED+IV TNP+LE++GNAKT RNDNSSRFGKFIRI FN GKLSG D+
Sbjct: 1 MPGHVKLGKSNEPDPDPMPYLVVSNEMRRNDMHKPYDAKKSVWVPD-LEGGYCEALLDSEEGGKSTVMIGHIK--KVYKTDEVAQVNPPKFEKCDDMANLTFLNDASVLWNLRNRYTSKLIYTYSGLFCVVVNPYKRFPIYTQSVVKIYLGKRRNEVPPHLWAITETAYRNMLTNTKDQSMLITGESGAGKTENTKKVISYLAMVASSGKKQTRKTTLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFNSSGKLSGCDI 262
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000012870 (supercontig:LSalAtl2s:LSalAtl2s97:2856074:2862588:-1 gene:EMLSAG00000012870 transcript:EMLSAT00000012870 description:"maker-LSalAtl2s97-augustus-gene-28.27") HSP 1 Score: 1551.95 bits (4017), Expect = 0.000e+0 Identity = 826/1767 (46.75%), Postives = 1181/1767 (66.84%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RR+E PPH++ + E +Y+ M+ K+QS+LITGESGAGKTENTKKVISY A V +SGK+ + + SLED+IV NP+L ++GNAKT RNDNSSRFGKFIRI F GKL+G D+ YLLEKSR+T Q +ER YH FY L+ V D+K KC LSD+IY Y +VSQGKVSV SIDD E++++ AF I+GF E E ++ YK+T+ VM G + F G+++QAE KV E+ G++ + ++ FCKPK+KVGTEWVTKGQTC QA++ V GIAR ++ +F+++ KCN+TL DPTMKK ++ LDIAGFEIF+YNGFEQI INF NEKLQQFFN HMFV+EQEEYV EGI+WA VDFGMDL I MFEKPMG+ AILEEESLFPKATD+SF KL L K F K K D +AHFA++HYA VSYN+T+WLEKNKDP+NDTVV++ K SN LL+ +R+HPGQ D G +K GGGKTVSS Y QL DLM TL+ T+P FIRC+VPNTHKKP VE L+MHQ CNGVL GI IC +GFPN+M Y D+K+RY IL A +A AK +K A+++ + EK+RLGHTKVFFRAG L +EE R+ V +++ W+Q Q G R +KK DQ+ L QR + Y + W W+ + +P + + + EE+ A +AL+ K++E + + + L+ L+S + D+ + + A+++ +L + +A E+ R + + K+ L+++I +E + EQ+K +D IR+L EE+ E+I K+ +EK+ + DN+ K ED+Q EDK +HL+ +K KLE +LDE ED++E EK+ K + EK+KRK+ES+L++TQE+++DL++ K EL + RKE E + + +K++DE +L K KQ KELQ R+EE +EEL ER +RAKAE+ RS L +++++LG R +EAG+ T Q ELNKKRE E+H+++ ++EE NI E L+ L+ KH + + E+ EQI+ L+ K K EKDK ++ ++ +AR++ ++ +RA++ +++ K + G + + N KL+E L + ES K++L E DL R + + N + K K SL + LE+ K +AD E+ +R L+ K+KNL EL+ RE++E E ++D + +SK + E+ LW+S++ETE + + +ELE ++ KL +R+ EAE T+E LN+K+ +KSR +LQ + +EMS +++ EK+ R FD+++ EWK K D L+ +++ S K+CRN +SELFR I+ L + +GGRSIHE+DK R+RLE EK ELQAALEEAE ALEQEENKVLRSQLEL QVRQEI+R+IQEK+EEF +KN +A++ + +LE E + K EALR+KKKLE D+ ELE++L+ AN N E QK++K+YQ Q+R++ E+E R + ++ I++R+ + L +EE+R LL ++R++R L+ EL +S ++E + N + KR E+ + +QA++D+M +A S+EKA +AM+DAARLADELR+EQEH++ +R ++ +ESQ ++ +L +AE + K G+ AM+K+E RIRELE EL + R +++ K +++ER +KEL F +EDRKN ERM L +LQ +IK+YK+QIEEAEEIAALNLAKFR++Q +E R+ +E++
Sbjct: 150 RRNEVPPHLWAITETAYRNMLQNSKDQSMLITGESGAGKTENTKKVISYLAMVASSGKKSQKKVSLEDQIVAXNPILXSYGNAKTSRNDNSSRFGKFIRIHFTPNGKLAGCDIESYLLEKSRITQQQSVERSYHIFYQLLQPFVADMKSKCCLSDDIYXYQYVSQGKVSVPSIDDNEELEFTDQAFDIIGFTENEKWDCYKITAAVMSFGEVK--FKQKGRDDQAEPDDLTYPNKVGELLGVNPDELMKSFCKPKIKVGTEWVTKGQTCEQATNGVGGIARGCFDRLFKWLIIKCNDTLIDPTMKKSHFVAVLDIAGFEIFEYNGFEQISINFVNEKLQQFFNHHMFVVEQEEYVSEGIDWAMVDFGMDLAAAIIMFEKPMGIWAILEEESLFPKATDKSFEEKLKAQHLGKSAPFAKPQSKTDKNAHFAIIHYAGIVSYNVTAWLEKNKDPVNDTVVDVLKRASNELLVFLWREHPGQS-NPPDDKGKKKKKGGGGKTVSSVYLVQLTDLMNTLHCTEPHFIRCIVPNTHKKPLEVEPPLIMHQLTCNGVLEGIRICMRGFPNRMLYPDYKSRYQILGAQEIATAKDNKTGVYALMDKISFPREKYRLGHTKVFFRAGALAQLEEDRDLIVLKLVRWIQGQFHGFIRRKDYKKRFDQRELLKVIQRNFKKYMSLRNWGWFIIIQKTRPLIGQLNMEEELKKLEEQXNEAYGAYTEALSVTKELEESNSTIKADINGLSKQLESEQGNLSQYQDRQIKANTLKAEIETELVKKQXLLAQEEVARQEMEVNRKKMMGDAQVLKKDIEDIEIVIQKVEQEKSNRDHTIRSLNEEVAESDELINKINKEKKHIGDNQSKAVEDLQGTEDKXNHLTSIKSKLETTLDELEDSVEREKRQKCEVEKIKRKLESELRMTQESVNDLDQQKKELEGTILRKESEASKLASKLEDEQSLVSKTQKQIKELQGRVEEYEEELEAERQARAKAERQRSDLAREIDELGERYDEAGSATHAQTELNKKRESEIHKLRRDVEEANIQQESLLSNLKKKHQDAIQEMMEQIEQLSKMKSKIEKDKVKIQHEIADARAATDEIIRARSSSEKSNKNLTGQLNELNKKLEEXNLTLGDFESAKRKLAAENSDLLRVVGDIGNNVNMLQKMKASLQSNLEEAKHIADHESHERFILVGKFKNLEHELDGMREQLEEEVCARNDLTRQISKCEGEVNLWRSKYETEAVAKSQELEMSKMKLTSRLTEAESTIENLNSKLQQIDKSRQKLQEEYDEMSYNFDQAQILHNQMEKKARQFDKIVNEWKLKVDGLSMDLDVSQKDCRNTSSELFR-------------------------IKILWTKSXEGGRSIHEIDKIRKRLEAEKLELQAALEEAEGALEQEENKVLRSQLELTQVRQEIERRIQEKDEEFEGIKKNQGKAVEGMQMALENESKGKAEALRMKKKLESDVTELEMSLEHANANNIETQKSIKKYQQQIRESQAKLEEEQRAKDMARDEQVISERRSHTLQNSLEEARTLLEQADRNRRTLEQELSDSNEVLSEYTVQNQAIVAAKRKLENELQNLQADLDEMNAEAHLSDEKASKAMIDAARLADELRNEQEHASKLERDRKLIESQSKDIHMKLDEAENASLKNGKKAMNKMETRIRELESELDAESRRMADAQKNLRKSERRIKELTFASDEDRKNHERMQSLVDQLQSRIKSYKKQIEEAEEIAALNLAKFRQTQSLLSSAEGRADLSEQA 1888 HSP 2 Score: 323.553 bits (828), Expect = 2.747e-89 Identity = 155/265 (58.49%), Postives = 196/265 (73.96%), Query Frame = 0
Query: 1 MPGHIKKS--DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGH KK PDPDP P+L++S E+K + KPYDPKKS W PD +GG+ EGL+ S + K V I +KK FK + VGQ+NPPKF+ +DM+ LTYLND V N R+K +LIYTYSGLFCI +NPYKR+PIYT R + +Y+GKRR+E PPH++ + E +Y+ M+ K+QS+LITGESGAGKTENTKKVISY A V +SGK+ + + SLED+IV NP+L ++GNAKT RNDNSSRFGKFIRI F GKL+G D+
Sbjct: 1 MPGHDKKGKPGEPDPDPEPYLVISMEMKREDMLKPYDPKKSVWAPD-GNGGFKEGLLVSDEDGKALVMI--GHEKKTFKSEAVGQINPPKFEKCEDMANLTYLNDGSVFHNLEARFKAKLIYTYSGLFCIVVNPYKRYPIYTPRVVKMYLGKRRNEVPPHLWAITETAYRNMLQNSKDQSMLITGESGAGKTENTKKVISYLAMVASSGKKSQKKVSLEDQIVAXNPILXSYGNAKTSRNDNSSRFGKFIRIHFTPNGKLAGCDI 262
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000005663 (supercontig:LSalAtl2s:LSalAtl2s3074:4839:11655:1 gene:EMLSAG00000005663 transcript:EMLSAT00000005663 description:"maker-LSalAtl2s3074-augustus-gene-0.2") HSP 1 Score: 1499.57 bits (3881), Expect = 0.000e+0 Identity = 817/1739 (46.98%), Postives = 1158/1739 (66.59%), Query Frame = 0
Query: 287 GKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGS-NNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRS 2024
GKNQS+LITGESGAGKTENTKKVISY A V +SGK+ + SLED+IV TNP+LE++GNAKT RNDNSSRFGKFIRI F GKL+G D+ YLLEKSR+T Q +ER YH FY L+ VP + K LL E ET+ + LT+ VM MG M F G+++QAE A KVA++ G++S+ M+ FCKPK+KVGTEWVTKGQTC QA++ V GIAR IY+ +F+++ KCN+TL D TMKK ++ LDIAGFEIF+YNGFEQI INF NEKLQQFFN HMFV+EQEEY+ EGI+W VDFGMDL MFEKPMG+ AILEEESLFPKATD+SF KL L K F K K D +AHFA++HYA VSYN+T WLEKNKDP+NDTVV++ K N LL+ ++DHPGQ ++ G +K GGGKTVSS Y QL DLM TL++T+P FIRC+VPNTHKKP VE L+MHQ CNGVL GI IC +GFPN+M Y DFK+RY IL AA ++ + +K A+++ VK + EK+RLGHTKVFFRAG L +EE R++ V +++ ++Q Q G R+ +KK DQ+ L QR R Y + W W+ + +P + + EEK A + L ++K+E + +K +L ++S + ++ + AD++ QL E +R+ +++R T + ++ + ++++I +E + EQ+K +D IR+L +++ HQ E+I ++ +EK+ + + K+ E++Q EDK HL+K+K KLEQ+LDE ED+ E EK+ + + EK++RK+E +LK++QE+++DLER K E + R+EKE +A+N K++++ GK+ K KE Q R+EE++EEL ER +RAKAE+ R + ++LE+L RL EAG T+ Q+ELNKKRE E+ +++ ++EE +I E T+ L+ KH + +SE+ EQID LN K K +KDK ++ ++ + R++ ++ R+KA +++ K + + + N K+++ + + E+ K+R+ E DL RQ++E EN + K KI + +QLE+ KR+AD E +DR L +KY+NL EL+ +R ++E E K++ + +KA E +WKS+FE EG+ + EELE ++ KLQA + E + T+E LN K+ EKS+S LQ + E+MS + ++ H EK+ R F++++GEWK KAD + E++ S KECRN +SELFR++ A+EEAI QLD V+RENK L++EI+D++DQ+ +GGRSIHE+DK R+RLE EK ELQAALEEAE ALEQEENKVLRSQLEL QVRQEI+R+I EKEEEF TRKN +A+D + +LE E ++K EALR+KKKLE D++ELE +L+ AN AN E QK +K+Y Q+R++ EDE + ++ ++ ADRK +A +EE+R LL ++R +R + +L ++ ++E++ N +R + I + AE+D+M +A+ S++KA++AM+DAA+LADELR EQE + +R + LE Q +L+ R+ + EAN K GR AM+K+E RIRELE EL S Q R ++S K +++ERH+KEL + +EDRKN ERM L +LQ KI++YK+QIEEAEEI A+NLAKFR++Q ESE+R+
Sbjct: 187 GKNQSMLITGESGAGKTENTKKVISYLAMVASSGKKAAKKVSLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFTTSGKLAGCDIESYLLEKSRITQQQAVERSYHIFYQLLQPFVPXYEGKMLLG--------------------------------------ERETWECFMLTAAVMSMGEMK--FKQKGRDDQAEPDDLTFANKVADLMGVNSDEMMKAFCKPKIKVGTEWVTKGQTCDQANNGVGGIARSIYDRIFKWLIIKCNDTLIDTTMKKSNFVAVLDIAGFEIFEYNGFEQISINFVNEKLQQFFNHHMFVVEQEEYISEGIDWXMVDFGMDLAAAXIMFEKPMGIWAILEEESLFPKATDRSFEEKLKAQHLGKSAPFAKPQSKTDKNAHFAIIHYAGIVSYNVTGWLEKNKDPVNDTVVDVLKRSPVNELLVVLWKDHPGQS-SPPEEKGKKKKKGGGGKTVSSVYLVQLTDLMNTLHSTEPHFIRCIVPNTHKKPLEVEPPLIMHQLTCNGVLEGIRICMRGFPNRMLYPDFKSRYQILGAAEISSSGDNKTGVFALMDKVKFDREKYRLGHTKVFFRAGALAQLEEARDEIVLKLVRFMQGQFYGFLKRVEYKKKADQRELLKVIQRNFRKYQTLRNWGWFIIIQKTRPLIGQVNIEEELRLLEEKAKEAYGAYEEQLKTKEKLEQESIKIAEEKKKLFAQIESEQGNLSQYTERQAKASAQRADLEVQLQESGDRLTLMERERHDATCNKKSLEQENVVIKKDIEDLELCIHRLEQEKTNRDHAIRSLNDDITHQDEIINRMNKEKKHMDEYSSKSSEELQTAEDKVEHLNKIKSKLEQTLDELEDSFEREKRGRAEIEKMRRKLEGELKVSQESVADLERSKREAENTIGRREKEISALNNKLEEDQCGVGKHQKHIKETQCRVEEMEEELEAERQARAKAERQRGDMARELEELSERLIEAGGATSAQIELNKKRESEVSKMRKDLEEIHIQQEATMQNLKRKHQDAVSEMSEQIDQLNKMKSKIDKDKYHIHSEIGDVRAAGDEISRSKAAAEKSNKSLLAQLNELNKKVEDSKLTVGDYENSKRRIGAENSDLLRQLQELENNASMLAKYKIQMASQLEEAKRVADDECKDRQLLFSKYRNLEHELDGTRYQLEEEASSKAELQRQFTKASHEADMWKSKFEKEGLAKAEELEMSKMKLQAXLXEXQGTIEQLNGKLGQLEKSKSTLQSEFEDMSAQADQAHILNSSMEKKARQFEKIVGEWKVKADSFSRELDNSQKECRNASSELFRVKNAYEEAIIQLDEVRRENKQLSNEIKDIMDQISEGGRSIHEIDKIRKRLESEKTELQAALEEAEGALEQEENKVLRSQLELNQVRQEIERRICEKEEEFQCTRKNFGKAIDQMQTALENETKSKAEALRMKKKLEADVSELETSLEHANAANMETQKTIKKYHQQIRESQLRLEDEQQAKEMARDEFLAADRKAHAHQNALEEARTLLEQADRVRRMTEQDLSDTNEQLSELTCQNQAIAGAQRKLGAEIQTLNAELDEMSAEARMSDDKAQKAMIDAAKLADELRREQEIAQLHERDCKVLECQAKDLQARVDETEANMLKSGRKAMNKMETRIRELESELDSEQRRLADSYKNLRKSERHIKELTYATDEDRKNHERMQGLIDQLQSKIRSYKKQIEEAEEIXAINLAKFRQTQSNLAESEERA 1884 HSP 2 Score: 317.775 bits (813), Expect = 2.092e-87 Identity = 152/265 (57.36%), Postives = 181/265 (68.30%), Query Frame = 0
Query: 1 MPGHIK--KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIK S+ PDPDP P+L+VS E+K + +KPYDPKKS WVP G+ EGL+DS G K V + +KK FK DQVGQVNPPKF+ +DM+ LTYLNDA V N RY+ +LIYTYSGLFC+ +NPYKR+PIYT P + GKNQS+LITGESGAGKTENTKKVISY A V +SGK+ + SLED+IV TNP+LE++GNAKT RNDNSSRFGKFIRI F GKL+G D+
Sbjct: 39 MPGHIKLGSSNEPDPDPLPYLVVSNEMKRQDMAKPYDPKKSVWVPTDNGQGFVEGLLDSETGGKSIVMV--GHEKKTFKPDQVGQVNPPKFEKCEDMANLTYLNDASVFNNLKTRYQAKLIYTYSGLFCVVVNPYKRYPIYT----------------PTVVXXX---------XGKNQSMLITGESGAGKTENTKKVISYLAMVASSGKKAAKKVSLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFTTSGKLAGCDI 276
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000004337 (supercontig:LSalAtl2s:LSalAtl2s228:112323:140864:1 gene:EMLSAG00000004337 transcript:EMLSAT00000004337 description:"maker-LSalAtl2s228-snap-gene-1.15") HSP 1 Score: 1474.92 bits (3817), Expect = 0.000e+0 Identity = 810/1703 (47.56%), Postives = 1150/1703 (67.53%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGA-SGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ---PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK-KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIA------EKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQ 1955
RR+E PPH+F V++ +Y+ M++ GESGAGKTENTKKVI+YFA VGA K+ + + SLED+IVQTNP+LEA+GNAKT RNDNSSR KL+G D+ YLLEKSR+TFQ E+ER YH FY + AVPDLK C LS++IYDY +VSQGK SV SIDD ED+++ HDAF IL F+ EETYN+YK+T+ VMHMG M F G+EEQ E + D A KV ++ G+D E +I FCKPK+KVGTEWVTKGQ Q++SSVAG+AR +Y+ +FRF+ +KCN TL D +MKKV +IG LDIAGFEIF+YNGFEQ+CINFCNEKLQQFFN HMFVLEQEEY++EGI+W VDFGMDLQ CI MFEKPMG+LAILEEESLFPKATD++F KL N L K NF KAS K D AHFA+VHYA TVSYNLT WLEKNKDPLN+TVVELFKNGSN L + F DHPGQ P + K GG + KGG KTVSSFYK QLD LM TL+AT+P FIRC+VPN +K PG ++S LV+HQ CNGVL GI IC +GFPN+M + DF +RY IL + + + KD ++ K++ EKFR+GHTK+FFRAG+LGY+EEVR+D V +++ +LQ Q G +R + + + Q+ L QR R Y + W W+ + +P + + E++ +A EK + +AL +++ ++ LL +K+ L +K + ++N + AD++ QL + +++A E++++ ++ Q+ ++ + LR E++ +E ++ AE +K +D +R+L +++ +Q E+I+KL +EK+ +Q+N K +D+Q +DK SHL+ VK KLEQ++DE E+ALE EK+ +NDSEK KRK E++LK++QE ++DLER K E ++ R+EK+ MN+K++ E + GK ++ KELQ+R+EE +EEL ER RAK+E+ RS L ++LE+L RLEEA TA Q+ELNKKRE E R++ ++EE +I E + +L+ KH++ +SE+ EQID LN K KAE +K ++ + +++ + + KA ++N K +Q + N K+ E + L + E + K+L M +L R +++ E+ ++ NK KI LT QL+D KRL D EA++R SLL +Y+NL E + +R +E E K D ++ KA+ E W+ ++E +G+ ++EELE ++ KLQAR+ E E T+E LNNK++ +K++++LQ D+EE E + + +K+ R FD++I EWK K D L+ E++ S KECRN +SELFR++ +EEA Q VK+EN NL DEI+D+++Q+ +GGRSIHE++KQR+RLE EK+ELQ+ALEEAE+ALE EENK LR+Q+E+ QVRQE++R+I EK+EEF +K+H + + + SLEAE +AK E LR KKKLE DI ELE AL+ AN +AE QK + +YQ +R T EDE + + ++E + +DR+ ++L +EE++ LL ++R++R + EL + S+N++S N KR +S I+ E++ M ++A +EEKAK AM+DAA+LA+ELR+EQ+ + + ++A+E+Q+ +L+ KLE RIRELE EL Q R ++ + Q
Sbjct: 150 RRTEVPPHLFAVSDTAYRNMLSS--------KGESGAGKTENTKKVIAYFAMVGAREDKKSKVKVSLEDQIVQTNPILEAFGNAKTARNDNSSR-------------KLAGCDIETYLLEKSRITFQQEVERSYHIFYQMFQKAVPDLKDACHLSNDIYDYHYVSQGKTSVPSIDDNEDLEFTHDAFNILHFSNEETYNIYKITAAVMHMGEMK--FKQKGREEQCEPDAMDKAEKVGDLLGVDPETLIKSFCKPKIKVGTEWVTKGQNIEQSTSSVAGVARGLYDRIFRFLVEKCNLTLVDKSMKKVFFIGVLDIAGFEIFNYNGFEQLCINFCNEKLQQFFNHHMFVLEQEEYLKEGIDWEMVDFGMDLQSCITMFEKPMGILAILEEESLFPKATDKTFEDKLKTNHLGKSSNFTKASTKTDKSAHFAIVHYAGTVSYNLTGWLEKNKDPLNETVVELFKNGSNKLTVHIFADHPGQGSQPHDDAKGKKGGKKAKGGHKTVSSFYKLQLDSLMSTLHATEPHFIRCIVPNGNKAPGEIDSALVLHQLTCNGVLEGIRICMRGFPNRMPFSDFCSRYMILENSKIKSSNMKDPQKITELICTSKIDKEKFRVGHTKIFFRAGVLGYLEEVRDDIVLKLVRFLQGQIFGLLARREYSRKKKQREYLKVIQRNFRKYMRLRNWGWFSIIQKTRPLIGMVNIEEEIKVLEDQAQMAVEEVENEKKVTEAL-EKENID-----LLEKKAALLKRVKLEQGDLSTYQERNAKASAQKADLEAQLIDSQDKLANEEKKKHQISSQKKSLEKDVNNLRREVSELEEQIIRAENEKASRDHTLRSLNDDITNQDEIISKLNKEKKYIQENNNKIGDDLQVADDKVSHLNMVKSKLEQTMDEMEEALEKEKRYRNDSEKNKRKFETELKVSQEHVADLERSKKESESSLIRREKDILEMNSKLECEQSQAGKLTRNIKELQARVEEWEEELEAERQGRAKSERQRSDLNRELEELTERLEEASGATAAQIELNKKREAEXLRLRKDLEEASIQQEAIILSLKKKHHDAISEMTEQIDQLNKLKSKAENEKMTIKMQTDDLKAAHDHLMAEKASAEKNNKNLQSQNMNINKKIAECSMQLQDLEERNKKLLMGNSELLRCLDDVESNISIMNKSKIELTNQLDDAKRLCDDEAKERQSLLGRYRNLEHEYDGTRAILEEEISAKEDLIRQFKKAENETCHWRLKYEQDGIAKIEELENSKLKLQARLTECEGTLENLNNKMIQLDKAKTKLQKDIEEFGTEVDHANIKNGQIDKKIRQFDKIIIEWKQKTDHLSSELDNSQKECRNVSSELFRVKGGYEEATNQFSEVKKENMNLTDEIKDIMEQINEGGRSIHEIEKQRKRLESEKKELQSALEEAESALESEENKNLRAQMEINQVRQELERRINEKDEEFEMVKKSHIKLAEQMQNSLEAESKAKAETLRSKKKLEADIQELERALEHANITHAENQKNISKYQDNIRSTTLRLEDEQKTKGMMRENLISSDRRTHSLQNSLEEAKTLLEQADRARRAAEHELNDCHESMNDLSVQNQSLAATKRKIQSETDNIKQEVEYMNSEATMAEEKAKNAMMDAAKLAEELRAEQDMTIKIENERKAIEAQVKDLQV----------------AQKLETRIRELEGELDGEQRRLTDCFRKAQ 1807 HSP 2 Score: 752.281 bits (1941), Expect = 0.000e+0 Identity = 414/947 (43.72%), Postives = 593/947 (62.62%), Query Frame = 0
Query: 388 FQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVL-NVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEK 1333
FQA+L Y + ++ V K+ + + + + VSQGKV VESIDD E+++Y AF I+G +EE +N YKLT+ VM MG M F G+++QAE + K+A + G++ E M+ F KP++KVGTEWVTKGQ QA+++V GIAR I++ +F+++ KCN+TL D TMKK ++ LDIAGFEIF+YNGFEQI INF NEKLQQFFN HMFV+EQEEY+ EGI+W VDFGMDL MFEKPMG+ AILEEESLFPKATD+SF KL L K F K K D +AHFA++HYA VSYN+T WLEKNKDP+NDTVV+L K GSN+LL+ +R+HPGQ + G +K GGKTVSS Y QL+ LM TL+ T+P FIRC+VPNTHKKP VE L+MHQ CNGVL GI IC +GFPN++ Y DFK RY IL A+ + K + K +A+ +L N + EK++LGHTKVFFRAG L +EE R+D V ++ LQ G R +K QK + QR R Y + W W+ + +P + + EEK A + L+ ++ ++ + L + L +K+ + +K + AD + QL+E ++ E++ ++ + +++ + + ++ ++ L +E +K + ++ +R L +E+ H E+I+KL ++K+ + D + E++ DK +HL+ +K KLE++LD+ E ALE EK+ K+ EK +RK+E DLK++QE + DLER K EL + RK+ E + +DDE + + K KEL SR+EE++EEL ER R+KAE+ ++ L ++ ++L RL E G TA Q+ELNKKRE E+ +++ ++EE NI E TL +L+ KH ++M+ + EQ D+LN + K EK
Sbjct: 1948 FQAKLIYTYSGLFCVV---VNPYKRYPIYTPRVVKIY-VSQGKVKVESIDDNEELEYTDSAFDIIGLTQEEKWNCYKLTAAVMSMGEMK--FKQKGRDDQAEPDGFEIPSKIATLFGVECENMMKCFVKPRIKVGTEWVTKGQNIEQATNAVGGIARAIFDRLFKWLIIKCNDTLIDTTMKKSNFVAVLDIAGFEIFEYNGFEQISINFVNEKLQQFFNHHMFVVEQEEYLAEGIDWVMVDFGMDLAXAXIMFEKPMGIWAILEEESLFPKATDRSFEEKLKAQHLGKSAPFAKPQSKTDKNAHFAIIHYAGIVSYNVTGWLEKNKDPVNDTVVDLLKKGSNDLLVFLWREHPGQTAPPPDEGGKKKKKGSGGKTVSSVYLVQLNSLMSTLHKTEPHFIRCIVPNTHKKPLEVEPPLIMHQLTCNGVLEGIRICMRGFPNRILYHDFKQRYAILGASKIDKNTEMKKSAEIILANTKGFDPEKYKLGHTKVFFRAGALAVLEEKRDDIVTLLIRKLQGTVYGHLKRKEYKIRYQQKEFIKVIQRNFRKYKDHRDWPWFIIIQKTRPLIGVVNVEEELRILEEKAKAAYGAYQEQLSTKETLQNENNVLNTELVGLRETIKNEQGDLGLYQEKMAKFSAQKADFEHQLNEACEKLEREQRSKEYVQEEKKDAEREVQNIKHVYQDAKSCLEKSELEKQKLEQIMRGLNDEVLHSDEIISKLNKDKKHLNDTMSRAVEELVGNTDKVNHLNDIKAKLEKTLDQMEGALEKEKRNKSIIEKERRKMEGDLKISQEYVLDLERAKKELEHCILRKDTEINQVTTYLDDEQSGVSRIQKSIKELTSRVEEMEEELEAERQGRSKAERQKADLAREFDELAERLXEXGIATAAQIELNKKREYEISKMRKDVEEINIQQESTLLSLKKKHQDSMAXMNEQXDHLNKIRXKLEK 2888 HSP 3 Score: 302.753 bits (774), Expect = 1.510e-82 Identity = 147/266 (55.26%), Postives = 188/266 (70.68%), Query Frame = 0
Query: 1 MPGHIK--KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGA-SGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPG+IK S+ PDPDP+P+L VS ++K + KPYD KKS W P + GG+ EGL+ S DG K V + +KKV K DQV QVNPPKF+ +DMS LTYLN+A VLWN RY ++IYTYSGLFC+A+NPY+R+PIYT ++ LY+GKRR+E PPH+F V++ +Y+ M++ GESGAGKTENTKKVI+YFA VGA K+ + + SLED+IVQTNP+LEA+GNAKT RNDNSSR KL+G D+
Sbjct: 1 MPGNIKLGASNEPDPDPAPFLYVSFDMKRNDQLKPYDAKKSVWCPGE-EGGFVEGLLQSDDGKKAVVLV--GHEKKVCKSDQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKSRYIAKMIYTYSGLFCVAVNPYQRYPIYTPTTVKLYLGKRRTEVPPHLFAVSDTAYRNMLSS--------KGESGAGKTENTKKVIAYFAMVGAREDKKSKVKVSLEDQIVQTNPILEAFGNAKTARNDNSSR-------------KLAGCDI 242 HSP 4 Score: 184.496 bits (467), Expect = 1.771e-46 Identity = 82/144 (56.94%), Postives = 104/144 (72.22%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGK 150
KS+ PDPDP+P+LIVS E+K + KPYD KKS WVPD GG+ EGL+DS DG K V +KK FK + VGQVNPPKF+ +DM+ LTYLNDA V N R++ +LIYTYSGLFC+ +NPYKR+PIYT R + +Y+ +
Sbjct: 1844 KSNEPDPDPAPFLIVSMEMKREDMLKPYDSKKSVWVPD-GQGGFREGLLDSVDGGKSNVMC--GHEKKXFKNEDVGQVNPPKFEKCEDMANLTYLNDASVFNNLKTRFQAKLIYTYSGLFCVVVNPYKRYPIYTPRVVKIYVSQ 1984 HSP 5 Score: 54.6842 bits (130), Expect = 3.801e-7 Identity = 197/922 (21.37%), Postives = 378/922 (41.00%), Query Frame = 0
Query: 1121 EEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEME-------KMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRE-RIENEHERKS----------DHLKALSKAQAEIQLWKSRFETEGMGRVEELE-----------GTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNF-------DRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHE----KRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEI 2002
EE+++ LED++ A + +E+EKKV EK ++ L ++ + L+RVK E +E+ K S Q +L+++L + ++L E + + + L+KD+ +L + E E I+AE E+ + H TL +L N+ ++ E I LN K +++ + DLQ A + K++L++ ++ + L++ R N++E K++ E E DLER +E E+++ + K + + ++LE + A R+ L + + ELE+ R+ R ++E +R +A A+I+L K R E E + ++LE + K I E E ++ LN +E + +++ +++ ++ A +EK +N ++ I E + DL E NK+ NSEL R E I ++ K E N D+ + L D D + L + R LE E + +A LEE +A E + +++ E R + ++ K EE N++ Q + +LE + + K KL+ DI E +D AN N + K ++++ + + Q + S Q+ + + G EE+ ++ L E+++ +NE R++HE ++ ES +Q+ +++ + ++ E K RA ++ ++ EL E + +L E +AE+ A + KLE I+ELE L +E+ K + + +++ + E+++K + M E ++ + + +EEA+ +
Sbjct: 828 EEEIKVLEDQAQM--------------AVEEVENEKKVTEALEK------ENIDLLEKKAALLKRVKLEQGDLSTYQER---------------NAKASAQKADLEAQLIDSQDKLANEEKKKHQISSQKKSLEKDVNNLRREVSEL----------------EEQIIRAENEKASRDH--TLRSL----NDDITNQDEIISKLNKEKKYIQENNNKIGDDLQVADDKVSHLNMVKSKLEQ-------TMDEMEEALEKEKRYRNDSEKNKRKFETELKVSQEHVADLERSKKESESSLIRREKDILEMNSKLECEQSQAGKLTRNIKELQARVEEWEEELEAERQGRAKSERQRSDLNRELEELTERLEEASGATAAQIELNKKR-EAEXLRLRKDLEEASIQQEAIILSLKKKHHDAISEMTEQIDQLNKLKSKAENEKMTIKMQTDDLKAAHDHLMAEKASAEKNNKNLQSQNMNINKKIAECSMQLQDL----EERNKKLLMGNSELLRCLDDVESNISIMNKSKIELTNQLDDAKRLCD---DEAKERQSLLGRYRNLEHEYDGTRAILEEEISAKEDLIRQFKKAENETCHWRLKYEQDGIAKIEELENSKLKLQARLTECEGTLENLNNKMIQLDKAKTKLQKDIEEFGTEVDHANIKNGQIDKKIRQFDKIIIEWKQKTDHLSSELDNSQKECRNVSSELFRVKGGYEEATNQFSEVKKENMNLTDEIKDIMEQINE----GGRSIHEIEKQRKRLESEKKELQSALEEAESALESEENKNLRAQMEINQVRQELERRINEKDEEFEMVKKSHIKLAEQMQNSLEAESKAKAETLRSKKKLEADIQELERALEHANITHAENQKNISKYQDNIRSTTLRLEDEQKTKGMMRENLISSDRRTHSLQNSLEEAKTL 1673
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|110825729|sp|P05661.4|MYSA_DROME (RecName: Full=Myosin heavy chain, muscle) HSP 1 Score: 1805.8 bits (4676), Expect = 0.000e+0 Identity = 973/1779 (54.69%), Postives = 1297/1779 (72.91%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG---EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPH--AHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGK-------TVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RR+E PPHIF +++G+Y M+ NQS+LITGESGAGKTENTKKVI+YFA+VGAS K E + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS +VP +K CLL+DNIYDY VSQGKV+V SIDD E+ AF ILGF ++E +VY++T+ VMHMG M F G+EEQAE E+ +V+++ G D+ + KP++KVG E+VT+G+ Q ++S+ + + +++ +F+++ KCNETL D K+ +IG LDIAGFEIF+YNGFEQ+CINF NEKLQQFFN MFV+EQEEY +EGI W +DFGMDL CID+ EKPMG+L+ILEEES+FPKATDQ+F+ KL L K FQK P AHFA+ HYA VSYN+T WLEKNKDPLNDTVV+ FK N LLIE F DH GQ +GGG + KGG TVSS YK QL+ LM TL +T P F+RC++PN K+PG V++ LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL + K A+K ++ +L + +RLGHTKVFFRAG+LG MEE R++R+G+++SW+Q+ ARG SR FKK+Q+Q++AL QR +R Y +TW W++LW +KP L ++ A EEK AE+ + RK++E ++ LL +K+ L +L A+QD ++N ++ D++ QL ++ R+ E+ R+ L QQ+ K + S L+++I +E + AEQDK KD QIR L +E+ HQ E+I KL +EK+ + QKT E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKKV+ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE + K+ +Q KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HE TLA LR KHN+ ++E+ EQ+D LN K KAE D+ +L + R++ + R KA ++ K +Q ++ + SKLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K KISLTTQLEDTKRLAD E+R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE Q+W+S++E++G+ R EELE + KLQAR+ EAEET+E+LN K + EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLR+QLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+D E+E R R + +E +GI++R+ NAL E+EESR LL ++R +RQ + EL ++ +NE+S+ N KR ES + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALE Q+ EL+ RL +AEANA K G+ A+ KLE R+RELE EL Q R +++ K +++ER VKEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 147 RRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMK--FKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETL-DTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQ-------SGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPRGIKDLDCPKKASKVLIESTELNEDLYRLGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1915 HSP 2 Score: 330.487 bits (846), Expect = 7.668e-90 Identity = 156/266 (58.65%), Postives = 197/266 (74.06%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG---EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MP + ++ D DP+P+L VS E + +SKPYD KKSCW+PD+ G Y G I +T GD V+V L+ + + K ++V +VNPPKF+ +DM+ +T LN CVL N RY +LIYTYSGLFC+AINPYKR+P+YT R +Y GKRR+E PPHIF +++G+Y M+ NQS+LITGESGAGKTENTKKVI+YFA+VGAS K E + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 1 MPKPV--ANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEG-YLLGEIKATKGDIVSVG-LQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 262
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|127773|sp|P24733.1|MYS_ARGIR (RecName: Full=Myosin heavy chain, striated muscle) HSP 1 Score: 1595.1 bits (4129), Expect = 0.000e+0 Identity = 848/1777 (47.72%), Postives = 1229/1777 (69.16%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEAS------LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPH---AHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKD-KDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
R++E PPH+F VA+ +YQ M+ +NQS LITGESGAGKTENTKKVI Y A V + K+K+ EAS LED+I+Q NPVLEA+GNAKT RN+NSSRFGKFIRI F GK++GAD+ YLLEKSR+T+Q ER YH FY + S+A+P+L L++ + Y +++QG ++V++IDD E+ + +AF ILGF +EE +++K T+ ++HMG M F +EEQAE A KVA +CGI++ ++ KPK+KVGTE VTKGQ +Q +SV +A+ +Y+ +F ++ + N+TL D K+ YIG LDIAGFEIFD+N FEQ+CIN+ NE+LQQFFN HMF+LEQEEY +EGI W +DFGMDLQ CID+ EKPMG+L+ILEEE +FPKA D+SF KL++N + K F K P+ AHF + HYA V Y++T WLEKNKDP+N+ VV L L+ E F+ P E K +T+S+ ++ L+ LMK LY+T P F+RC++PN K+PG V++ LV+HQ QCNGVL GI ICRKGFP+++ Y +FK RY+ILA A+ + D K ++ +L ++++ ++RLG TKVFF+AG+LG +EE+R++R+ +++S Q+ RG R +KK+QDQ++ L QR IR + + + W WW+L+ +KP L + + E +++ ++++ K +K++E + LL QK++L L L++ ++ D ++ ++ AD + Q+ E+ R+ E+ L + K+++ + L+++I +E L AEQDK KD QI TL+ E+ Q E I KL +EK+ +++ +KT + +QA EDK +HL+K+K KLEQ+LDE ED LE EKKV+ D EK KRK+E DLK TQE + DLERVK EL + V+RKE E +++N+K++DE L + ++ KELQ+R+EEL+EEL ER +RAK EK R+ L ++LE+LG RL+EAG T+ Q+ELNKKRE EL +I+ ++EE ++ HE ++ALR KH + +E+ +Q+D L K K EKDK +++R++ + S + ++ K ++ K + + D N++L++ R++NE +SQK RL+ E DL RQ+E+ E+ ++ +K+K L++QLED +R + E R RS L + +N+ ++++ RE++E E E KSD + LSKA EIQ W+S+FE+EG R EELE + KL ++ EAE+T EA N K + EK++SRLQ +LE+MS+E +R +A+ EK+ R FD+ EW+AK + L E+E S KE R Y++EL+R++A+ EE + + ++RENKNLADEI DL DQL +GGRS HELDK RRRLE+EKEELQAALEEAE ALEQEE KV+R+QLE+ VR EID++IQEKEEEF+NTR+NHQRA++S+ ASLEAE + K +A+RIKKKLE DINELE+ALD +N+ AE +K VKRYQ Q+R+ E+E R R E +E+ +A+R+ ++GEVEE RA L +ER+++ D EL ++ VNE++S + +KR E I+A+Q ++D+M + K ++E+ K+AM DAARLADELR+EQ+HS ++ ++ LESQ+ E + RL +AEA++ K G+ + KLE R+ ELE EL + Q R +E+ K ++A+R +KEL FQ +EDRKNQER+ EL KL KIKT+K+Q+EEAEEIAA+NLAK+RK+Q E EE+E+R+ TA+ +
Sbjct: 144 RKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIFYQICSNAIPELNDVMLVTPDSGLYSFINQGCLTVDNIDDVEEFKLCDEAFDILGFTKEEKQSMFKCTASILHMGEMK--FKQRPREEQAESDGTAEAEKVAFLCGINAGDLLKALLKPKVKVGTEMVTKGQNMNQVVNSVGALAKSLYDRMFNWLVRRVNKTL-DTKAKRNYYIGVLDIAGFEIFDFNSFEQLCINYTNERLQQFFNHHMFILEQEEYKKEGIAWEFIDFGMDLQMCIDLIEKPMGILSILEEECMFPKADDKSFQDKLYQNHMGKNRMFTKPGKPTRPNQGPAHFELHHYAGNVPYSITGWLEKNKDPINENVVALLGASKEPLVAELFK----APEEPAGGGKKKKGKSSAFQTISAVHRESLNKLMKNLYSTHPHFVRCIIPNELKQPGLVDAELVLHQLQCNGVLEGIRICRKGFPSRLIYSEFKQRYSILAPNAIPQGFVDGKTVSEKILAGLQMDPAEYRLGTTKVFFKAGVLGNLEEMRDERLSKIISMFQAHIRGYLIRKAYKKLQDQRIGLSVIQRNIRKWLVLRNWQWWKLYSKVKPLLSIARQEEEMKEQLKQMDKMKEDLAKTERIKKELEEQNVTLLEQKNDLFLQLQTLEDSMGDQEERVEKLIMQKADFESQIKELEERLLDEEDAAADLEGIKKKMEADNANLKKDIGDLENTLQKAEQDKAHKDNQISTLQGEISQQDEHIGKLNKEKKALEEANKKTSDSLQAEEDKCNHLNKLKAKLEQALDELEDNLEREKKVRGDVEKAKRKVEQDLKSTQENVEDLERVKRELEENVRRKEAEISSLNSKLEDEQNLVSQLQRKIKELQARIEELEEELEAERNARAKVEKQRAELNRELEELGERLDEAGGATSAQIELNKKREAELLKIRRDLEEASLQHEAQISALRKKHQDAANEMADQVDQLQKVKSKLEKDKKDLKREMDDLESQMTHNMKNKGCSEKVMKQFESQMSDLNARLEDSQRSINELQSQKSRLQAENSDLTRQLEDAEHRVSVLSKEKSQLSSQLEDARRSLEEETRARSKLQNEVRNMHADMDAIREQLEEEQESKSDVQRQLSKANNEIQQWRSKFESEGANRTEELEDQKRKLLGKLSEAEQTTEAANAKCSALEKAKSRLQQELEDMSIEVDRANASVNQMEKKQRAFDKTTAEWQAKVNSLQSELENSQKESRGYSAELYRIKASIEEYQDSIGALRRENKNLADEIHDLTDQLSEGGRSTHELDKARRRLEMEKEELQAALEEAEGALEQEEAKVMRAQLEIATVRNEIDKRIQEKEEEFDNTRRNHQRALESMQASLEAEAKGKADAMRIKKKLEQDINELEVALDASNRGKAEMEKTVKRYQQQIREMQTSIEEEQRQRDEARESYNMAERRCTLMSGEVEELRAALEQAERARKASDNELADANDRVNELTSQVSSVQGQKRKLEGDINAMQTDLDEMHGELKGADERCKKAMADAARLADELRAEQDHSNQVEKVRKNLESQVKEFQIRLDEAEASSLKGGKKMIQKLESRVHELEAELDNEQRRHAETQKNMRKADRRLKELAFQADEDRKNQERLQELIDKLNAKIKTFKRQVEEAEEIAAINLAKYRKAQHELEEAEERADTADST 1913 HSP 2 Score: 302.753 bits (774), Expect = 2.750e-81 Identity = 154/312 (49.36%), Postives = 200/312 (64.10%), Query Frame = 0
Query: 9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA------SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM-------------RRSECPPHIFGVAEGSYQGMMNVGK--NQSILITGESG 299
D DPD +L V + K ++ +D KK+CWVPD+ G + I S+ GD++TV I+ + KKD + +NPPKF+ +DM+ +TYLN+A VL+N RY + LIYTYSGLFCIA+NPY+R PIYT I Y GKR++E PPH+F VA+ +YQ M+ +NQS LITGESGAGKTENTKKVI Y A V + K+K+ EA SLED+I+Q NPVLEA+GNAKT RN+NSSRFGKFIRI F GK++GAD+ + +E HIF YQ N N +L+T +SG
Sbjct: 4 DFSDPD-FQYLAVDRKKLMKEQTAAFDGKKNCWVPDEKEG-FASAEIQSSKGDEITVKIVADSSTRTVKKDDIQSMNPPKFEKLEDMANMTYLNEASVLYNLRSRYTSGLIYTYSGLFCIAVNPYRRLPIYTDSVIAKYRGKRKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIF------YQICSNAIPELNDVMLVTPDSG 307
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|125987844|sp|P79293.2|MYH7_PIG (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1510.35 bits (3909), Expect = 0.000e+0 Identity = 807/1776 (45.44%), Postives = 1204/1776 (67.79%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPG--QPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K P AHFA++HYA TV YN+ WL+KNKDPLN+TVV+L+K S LL F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM FKK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ E++RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ E+ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVMYATGALAKAVYEKMFNWMVTRINTTL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGRPEAHFALIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKLLSNLFANYAGADTPVEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMSVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 276.174 bits (705), Expect = 3.474e-73 Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0
Query: 14 DPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +P+L S + + + +++P+D KK +VPD + + I S +G KVT K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ INPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 11 EAAPYLRKSEKERLEAQTRPFDLKKDVYVPDDKEE-FVKAKILSREGGKVTAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|81871557|sp|Q91Z83.1|MYH7_MOUSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1508.43 bits (3904), Expect = 0.000e+0 Identity = 807/1774 (45.49%), Postives = 1202/1774 (67.76%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q S ++ +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K AHF++VHYA TV YN+ WL+KNKDPLN+TVV L++ S LL F ++ G A K G +K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM FKK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ + + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G+SIHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVSYAIGALAKSVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPADK-GKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 275.018 bits (702), Expect = 7.787e-73 Identity = 132/254 (51.97%), Postives = 176/254 (69.29%), Query Frame = 0
Query: 17 PWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
P+L S + + + +++P+D KK +VPD + + I S +G KVT ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKAKIVSREGGKVTA---ETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|75054114|sp|Q8MJU9.1|MYH7_HORSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1507.27 bits (3901), Expect = 0.000e+0 Identity = 805/1776 (45.33%), Postives = 1205/1776 (67.85%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I++ NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K NFQK + K P AHF+++HYA TV YN+ WL+KNKDPLN+TVV+L+K S +L F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L+ + ++ ++R GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG +RM FKK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ A ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E ++K ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +++EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAKGALAKAVYERMFNWMVARINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVDLYKKSSLKMLSNLFANYLGADAPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYRFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMKRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRDAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEVEQKRNAESIKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 272.707 bits (696), Expect = 3.782e-72 Identity = 130/253 (51.38%), Postives = 174/253 (68.77%), Query Frame = 0
Query: 16 SPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+P+L + + + + +++P+D KK +VPD + + I S +G K+T K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY +IYTYSGLFC+ INPYK P+YT + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I++ NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 13 APYLRKTEKERLEDQTRPFDLKKDVFVPDDKEE-FVKAKIISREGGKITAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYAAWMIYTYSGLFCVTINPYKWLPVYTAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|83304912|sp|P12883.5|MYH7_HUMAN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1506.89 bits (3900), Expect = 0.000e+0 Identity = 804/1776 (45.27%), Postives = 1203/1776 (67.74%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K NFQK + K P AHF+++HYA V YN+ WL+KNKDPLN+TVV L++ S LL F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L+ + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG +RM +KK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+N R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ EAEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 276.174 bits (705), Expect = 3.980e-73 Identity = 132/253 (52.17%), Postives = 175/253 (69.17%), Query Frame = 0
Query: 16 SPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+P+L S + + + +++P+D KK +VPD + + I S +G KVT K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ +NPYK P+YT + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 13 APYLRKSEKERLEAQTRPFDLKKDVFVPDDKQE-FVKAKIVSREGGKVTAETEYGKTVTV-KEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|125987843|sp|P49824.3|MYH7_CANFA (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1506.12 bits (3898), Expect = 0.000e+0 Identity = 804/1776 (45.27%), Postives = 1205/1776 (67.85%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K AHF+++HYA TV YN+ WL+KNKDPLN+TVV L++ S LL F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L+ + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM +KK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ A ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYATGALAKAVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVALYQKSSLKLLSNLFANYAGADAPVEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEYKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARIKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKGLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 275.789 bits (704), Expect = 5.450e-73 Identity = 132/254 (51.97%), Postives = 176/254 (69.29%), Query Frame = 0
Query: 17 PWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
P+L S + + + +++P+D KK +VPD + + I S +G KVT ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKAKIVSREGGKVTA---ETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|75055810|sp|Q9BE39.1|MYH7_BOVIN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1505.73 bits (3897), Expect = 0.000e+0 Identity = 809/1783 (45.37%), Postives = 1205/1783 (67.58%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPG--QPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIG-------KALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K NFQK + K P AHF+++HYA TV YN+ WL+KNKDPLN+TVV+L+K S +L F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM FKK+ +++ +L Q IR + K W W +L+ IKP LK AE E++IA+ ++ G K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++EL EQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LEQEE K+LR+QLE Q++ E++RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ E+ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTTEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVVYAKGALAKAVYERMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKMLSSLFANYAGFDTPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKS-------AETEKEIALMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELSEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEQEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRLAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 276.559 bits (706), Expect = 2.692e-73 Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0
Query: 14 DPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +P+L S + + + +++P+D KK +VPD + + I S +G KVT K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ INPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 11 EAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKATILSREGGKVTAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|317373582|sp|P13533.5|MYH6_HUMAN (RecName: Full=Myosin-6; AltName: Full=Myosin heavy chain 6; AltName: Full=Myosin heavy chain, cardiac muscle alpha isoform; Short=MyHC-alpha) HSP 1 Score: 1501.11 bits (3885), Expect = 0.000e+0 Identity = 806/1775 (45.41%), Postives = 1199/1775 (67.55%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKR------KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY +VSQG+VSV SIDD E++ AF +LGF EE VYKLT +MH GNM F +EEQAE ++A K A + G++S ++ C P++KVG E+VTKGQ+ Q S+ +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K AHF+++HYA TV YN+ WLEKNKDPLN+TVV L++ S L+ F + + GG +K +TVS+ ++ L+ LM L T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL A+ + + + + +L+ + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+QARG+ R+ FKK+ +++ AL Q IR + K W W +L+ IKP LK + + A +E+ ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+N R+ E++ LT ++ K++ + S+L+++I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+Q EDK + LSK K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++KE + N+KI+DE L + K+ KE Q+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + KL+E R+LN+ +Q+ +L+ E +L RQ+EE E ++Q + K+S T Q+ED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG+GG+++HEL+K R++LEVEK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NHQR +DSL SL+AE R++ E LR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ES + +Q+E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EED+KN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMK--FKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRIKETLEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKVRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHQRVVDSLQTSLDAETRSRNEVLRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQSEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELEGELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1916 HSP 2 Score: 269.24 bits (687), Expect = 4.383e-71 Identity = 128/242 (52.89%), Postives = 167/242 (69.01%), Query Frame = 0
Query: 30 KSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKR------KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+++P+D + C+VPD + + I S +G KV I ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 27 QTRPFDIRTECFVPDDKEE-FVKAKILSREGGKV---IAETENGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 264
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|3024204|sp|Q02566.2|MYH6_MOUSE (RecName: Full=Myosin-6; AltName: Full=Myosin heavy chain 6; AltName: Full=Myosin heavy chain, cardiac muscle alpha isoform; Short=MyHC-alpha) HSP 1 Score: 1495.33 bits (3870), Expect = 0.000e+0 Identity = 805/1775 (45.35%), Postives = 1198/1775 (67.49%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE------ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + E +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY +VSQG+VSV SIDD E++ AF +L F EE VYKLT +MH GNM F +EEQAE ++A K A + G++S ++ C P++KVG E+VTKGQ+ Q S+ +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKA+D +F AKL++N L K NFQK + K AHF++VHYA TV YN+ WLEKNKDPLN+TVV L++ S L+ F + GG +K +TVS+ ++ L+ LM L T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+QARG+ R+ FKK+ +++ AL Q IR + K W W +L+ IKP LK + + A +E+ + + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+++I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++KE + + N+KI+DE L + K+ KE Q+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + KL+E R+LN+ +Q+ +L+ E +L RQ+EE E ++Q + K+S T Q+ED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG+GG+++HEL+K R++LEVEK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L QAN+ +E QK +K Q L+DT +D +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ES + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EED+KN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMK--FKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1916 HSP 2 Score: 274.248 bits (700), Expect = 1.490e-72 Identity = 129/242 (53.31%), Postives = 168/242 (69.42%), Query Frame = 0
Query: 30 KSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKV--FKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE------ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+++P+D + C+VPD Y + + S +G KVT ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + E +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 27 QTRPFDIRTECFVPDDKEE-YVKAKVVSREGGKVTA---ETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325302753|ref|XP_023332945.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2667.88 bits (6914), Expect = 0.000e+0 Identity = 1289/1767 (72.95%), Postives = 1537/1767 (86.98%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVISYFA++ +SGKRKEGE SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADMV+YLLEKSRLT+Q LERCYHAFYN+MSDAVPDLK+KCLL+DNI DYW+VSQGK++V SIDDKEDMQ+A +AF ILGF +EE Y+VYK T+ +MHMGNMTKDFVPVGKEEQAEIK + N++KVA + GID EWMINYFCKPKLKVGTEWV+KG +CS A++SVAGIAR IYE FR + DKCNETL DPTMKKVQYIG LDIAGFEIFDYNGFEQICIN+ NEKLQQFFN HMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLA+ EEESLFPKATDQ+F KLH NLL K PNF K +P+PDP AHFAV+HYAATVSYNLT+WLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLE KD GG +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GL+MHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA VAKAK DK AA AVL + L+ EK+RLGHTKVFFRAGILG+MEEVRED++G VL+WLQS ARGKASRM FKK+QDQKLALY CQRTIRN+ IGKTWLWWQ+W IKP+LKCT+F +YK E+E+KIA+AE NI KA+ADR+KV+ +H+ LL QK+E++LAL SGGSAVQDIIDK RVE +A D+QKQ+ EV RI AE++Q+ S+ Q KV+SQ +QL+ E++ +E +L +EQ++ +KD+QIRTLK+E+ HQ +M+ KLG+EK+ D +QK EED+Q ++DK +HLS+VK KLEQ+LDEAEDALE EKK KND EK+KRK E D KLTQE + DLERVKAEL+Q + RKEKE++A++AKI+DE +LG KY KQ KELQ RLEELDEEL IER +RAKAEKSRS+LKKD+ED+ +RLEEAGANT TQVELNKKRE EL R+K E+EE NI HEGTLAALRMKHNNTMS+LGE ID+LN +K+KAEKDK +ERDL ++R+SLED V+++AEL+R GK++QGSI ++++KLDE+ARA+NEA+S KKRL +E DL RQIEE ENA+A NK K+SL TQLEDTKRL+++E++DRSSLLTKYK++TTE E+ RE+IENEH +KSD LK+LSKAQAEIQLW+SR+ETEGMGRVEELEG KL++RI EAEET+++L K+ + EKS+ R+ DL+E+S+EYER HAAA+I+EKRG+NFD+V+GEW+AKA D+A E+EASN ECRNY+SEL+R++A+H+EA EQLD+VKRENKNLADEI+DLLDQLGDGGRSIH+LDKQRRRLEVEKEELQ+ALEEAE ALEQEENKVLR+QLELGQVRQEIDR++ EK+EEF NTRKNH RAMDSL+ASLEAEQ+AK EALRIKKKLE DINELEIALD ANKAN+EG K +KRYQ QLR+++QG E+ESR RQ++ E VGIA+RK AL+ E+EESRALL S+ER KRQL+AEL ++R SVNEM SIN++ + KRA E +H IQAEID ++ AK+SEEK KRAMVDAARLADELR+EQ+HS +E R KR+LE+QLGELET+L +AE NA + G++A+S+LE ++RELE ELG Q RTSE+ K +Q+ ER +KEL FQQEED+KNQ++M+E+A KLQQKIK YKQQIEEAEEIAALNLAKFRK+QQ+FEE+E+R K A+++
Sbjct: 149 RRNECPPHIFAIAEGAYQGMMNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKRKEGEVSLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNIMSDAVPDLKEKCLLTDNILDYWYVSQGKITVPSIDDKEDMQFADEAFDILGFTKEEKYDVYKNTACMMHMGNMTKDFVPVGKEEQAEIKDDTNSIKVATLAGIDPEWMINYFCKPKLKVGTEWVSKGSSCSNAANSVAGIARAIYERTFRLVVDKCNETLIDPTMKKVQYIGVLDIAGFEIFDYNGFEQICINYVNEKLQQFFNSHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAVFEEESLFPKATDQTFCEKLHSNLLGKWPNFAKPNPRPDPDAHFAVIHYAATVSYNLTNWLEKNKDPLNDTIVEMIKNGSNSLMIQCFADHPGQPLETPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLIMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKLVAKAKNDKAAAGAVLESINLDKEKYRLGHTKVFFRAGILGFMEEVREDKIGSVLAWLQSGARGKASRMAFKKLQDQKLALYCCQRTIRNFRIGKTWLWWQIWSAIKPHLKCTQFGKYKEEFEQKIALAEANIDKAIADRQKVQAIHDNLLSQKNEVSLALTSGGSAVQDIIDKAVRVENIANDIQKQVAEVKKRIKAEEEQKSSIEDQMRKVESQINQLQSEVSTLERQLENSEQERSDKDDQIRTLKDEIAHQADMLAKLGKEKKSGIDFRQKVEEDIQTMDDKCNHLSRVKGKLEQALDEAEDALEREKKSKNDVEKLKRKCEGDFKLTQEAVVDLERVKAELAQTLARKEKEYSALSAKIEDEGSLGSKYQKQIKELQGRLEELDEELNIERSNRAKAEKSRSILKKDIEDIAARLEEAGANTGTQVELNKKREGELSRLKCELEELNIAHEGTLAALRMKHNNTMSDLGENIDSLNSSKVKAEKDKSGLERDLADSRASLEDAVKSRAELERTGKLLQGSIAESHTKLDELARAMNEADSTKKRLTVENQDLSRQIEELENALANMNKSKVSLATQLEDTKRLSESESKDRSSLLTKYKHMTTEFETYREKIENEHLKKSDALKSLSKAQAEIQLWRSRYETEGMGRVEELEGNNSKLRSRITEAEETIDSLQTKLANIEKSKQRIGEDLDEISMEYERVHAAALITEKRGKNFDKVLGEWQAKASDIACEVEASNNECRNYSSELYRVKASHDEATEQLDIVKRENKNLADEIKDLLDQLGDGGRSIHDLDKQRRRLEVEKEELQSALEEAEGALEQEENKVLRAQLELGQVRQEIDRRLAEKDEEFLNTRKNHDRAMDSLNASLEAEQKAKGEALRIKKKLEGDINELEIALDHANKANSEGMKTIKRYQSQLRESLQGLENESRIRQQIAEQVGIAERKAAALSAEMEESRALLDSAERGKRQLEAELADTRNSVNEMQSINSKEIAVKRALEGNLHTIQAEIDSLLQAAKSSEEKGKRAMVDAARLADELRAEQDHSAAESRIKRSLETQLGELETKLTEAEENAIRCGKSALSRLETKVRELEQELGCTQVRTSENMKGFQKTERKIKELLFQQEEDKKNQDKMSEIANKLQQKIKVYKQQIEEAEEIAALNLAKFRKAQQDFEENEERVKLADEA 1914 HSP 2 Score: 478.019 bits (1229), Expect = 2.733e-134 Identity = 209/263 (79.47%), Postives = 232/263 (88.21%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKKS GPDPDPSPWL VSPELK K K KPYDPKKS WV +KA GGY EGL++S DG KVTV + + KV+K+DQV QVNPPKFDCSDDM+ LT+L DACVLWNS +RY N+LIYTYSGLFCIAINPYKRFPIYT R++++Y+G+RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVISYFA++ +SGKRKEGE SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADM
Sbjct: 1 MPGHIKKSSGPDPDPSPWLCVSPELKEKNKLKPYDPKKSVWVVNKADGGYLEGLLESKDGAKVTVNV--NGEMKVYKEDQVCQVNPPKFDCSDDMANLTFLGDACVLWNSTIRYVNQLIYTYSGLFCIAINPYKRFPIYTLRTMEIYVGRRRNECPPHIFAIAEGAYQGMMNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKRKEGEVSLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325302843|ref|XP_023332992.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2655.55 bits (6882), Expect = 0.000e+0 Identity = 1283/1765 (72.69%), Postives = 1517/1765 (85.95%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVI+YFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADMV+YLLEKSRLT+Q LERCYHAFYN+MSDAVPDLK+KCLLSDNI DYW+VSQGK+SV SIDDKEDM +A +AF +LGF E+E Y+VYK T+ +MHMGNMTKDFVPVGKEEQAEIK + NA+KVA + GID+EWMINYFCKPKLKVGTEWV KG TCS A++SVAGIAR IYE FR + +KCNETL DPTMKKVQYIG LDIAGFEIFDYNGFEQICIN+ NEKLQQFFNQHMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLAI EEESLFPKATDQSF KLH NLL K NF K +P+PDP AHFAV+HYAA VSYNLT WLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLE KD GG +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GLVMHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA AVAK K DK AA AVL+ +KLE EK+RLGHTKVFFRAGILG+MEEVRED++GEVLSWLQ+ ARGKASRM FKK+QDQKLALY CQRTIRNYYIGKTWLWWQ+W+L+KP+LKC +F +YKAEYE+KIA+AE NI KA+A+ V +H+ L +K EL LAL SGGSAVQDIIDK R+EGM D+QKQ+DE RIAAE+ + Q KV + +LREEI +E E+D++ KD QI+TLKEE+ HQ E+I K+ REK+ V D +QK EED+Q++ED+ +HL+KVK KLEQSLDE EDALE EKK + D EK+KRKIE DLKLTQE +SDLERVKAELSQ +QRKEKE ++++AKI+DE TLGGKYSKQ KELQSR+EELDEEL IER +RAKAEK+RS+L +D+ED+G RLEEAG NT+TQ+ELNKKRE EL ++K+++EE NI HEGTLAA+R KHNNTMS++GEQID+LN K K++KDK MERDLQEAR+ L++ +R +A +++N KM Q I ++N+KLDE+ARALNEA+S K+L +E DL RQIEE ENA+A K K+SLTTQLEDTKRLADAE RDR+SLLTK+K L+TE+E+ R RIE E ERK+D L+ALSKAQAEIQLW+S+FETE +GR++ELE ++ KL R+ EAEET+++LN K+ ++EK++ R++ +LEEM +EYERTHAAA+I+EKRGRNFD+V+GEWKAKADDL E+EAS ECRNYNSE+FRL+AA+EE +EQLDVV+RENKNLADEI+DLLDQLGDGGRSIH+LDKQRRRLEVEKEELQAALEEAE+ALEQEENKVLR+QLELGQV+QEIDR+IQEKEEEF+NTRKNH RAMDS+ ASLE+EQRAK EALRIKKK+E DINELEIALD ANKAN E QK++KRYQGQLR++ YE+ESR R E+ E +ADR+ NAL GE+EE+R+LL S+ER K+Q +AEL ++R +VNEM++IN++A EKR E +H + AEIDDM+ QAKNSEEK+K+AM+DAARLADELR+EQ+H ++ + KRA E+QL ELE +L +A NA + GRAAM+KLE RIRELE+ELGS+QSRTS++AK +Q+ ER VKEL FQ +ED+KNQERM+ELA KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EESE+R++ AE
Sbjct: 149 RRNECPPHIFAIAEGAYQGMMNSGCNQSILITGESGAGKTENTKKVIAYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNIMSDAVPDLKEKCLLSDNILDYWYVSQGKISVPSIDDKEDMMFADEAFDVLGFTEDEKYDVYKNTACMMHMGNMTKDFVPVGKEEQAEIKDDTNAIKVAALAGIDAEWMINYFCKPKLKVGTEWVNKGSTCSAAANSVAGIARAIYERSFRIVVEKCNETLIDPTMKKVQYIGVLDIAGFEIFDYNGFEQICINYVNEKLQQFFNQHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAIFEEESLFPKATDQSFCEKLHSNLLGKWENFAKPNPRPDPDAHFAVIHYAAVVSYNLTGWLEKNKDPLNDTIVEMIKNGSNSLMIQCFADHPGQPLETPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLVMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKAVAKCKNDKSAAGAVLDAIKLEKEKYRLGHTKVFFRAGILGFMEEVREDKIGEVLSWLQAGARGKASRMQFKKLQDQKLALYCCQRTIRNYYIGKTWLWWQIWMLVKPHLKCMQFGKYKAEYEKKIAVAEANIDKAIAECDAVIKIHDRLENEKQELHLALTSGGSAVQDIIDKTNRLEGMKNDLQKQVDETKKRIAAEEDVIAGIQQAGNKVTADAHRLREEIKNLENAAEKCEEDRITKDNQIKTLKEEISHQEELIAKMQREKKSVGDGRQKVEEDIQSMEDRCNHLAKVKGKLEQSLDECEDALEREKKCRGDVEKMKRKIEGDLKLTQEAVSDLERVKAELSQTIQRKEKELSSLSAKIEDEQTLGGKYSKQIKELQSRIEELDEELAIERQNRAKAEKNRSILSRDIEDIGRRLEEAGCNTSTQIELNKKRESELVKLKSDLEEANIAHEGTLAAIRAKHNNTMSDMGEQIDSLNKMKAKSDKDKAGMERDLQEARAGLDESMRDRANIEKNCKMTQALIVESNTKLDELARALNEADSSNKKLSVEHQDLIRQIEETENAIATLGKNKVSLTTQLEDTKRLADAEGRDRASLLTKFKGLSTEVENLRMRIEEEAERKNDVLRALSKAQAEIQLWRSKFETEALGRIDELESSKAKLNVRVQEAEETIDSLNTKVSATEKTKHRIEAELEEMQMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDLLAELEASRTECRNYNSEVFRLKAAYEETVEQLDVVRRENKNLADEIKDLLDQLGDGGRSIHDLDKQRRRLEVEKEELQAALEEAESALEQEENKVLRAQLELGQVKQEIDRRIQEKEEEFDNTRKNHARAMDSMQASLESEQRAKAEALRIKKKIEGDINELEIALDHANKANNEAQKSIKRYQGQLRESECAYEEESRMRAEMTEKACLADRRANALHGEMEEARSLLDSAERGKKQTEAELADARNAVNEMNTINSKAASEKRRVEGIVHTMHAEIDDMLQQAKNSEEKSKKAMIDAARLADELRAEQDHVNTQSKAKRAFETQLVELENKLDEANENAMRGGRAAMAKLETRIRELEIELGSVQSRTSDNAKGHQKCERRVKELSFQIDEDKKNQERMSELATKLQQKIKTYKKQIEEAEEIAALNLAKFRKAQQELEESEERTRMAE 1912 HSP 2 Score: 486.108 bits (1250), Expect = 5.331e-137 Identity = 213/263 (80.99%), Postives = 236/263 (89.73%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKK+ GPDPDPSPWLIVSPELK K K+KPYDPKKS WVP+KA GG+ EGLI+S DG K++V + + KVFK+DQV QVNPPKFDCSDDM+ LTYL DACVLWNSV+RY NELIYTYSGLFCIAINPYKRFPIYT R+++LY GKRR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVI+YFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADM
Sbjct: 1 MPGHIKKTTGPDPDPSPWLIVSPELKEKNKAKPYDPKKSVWVPNKADGGFVEGLIESKDGAKISVNV--KGEVKVFKEDQVCQVNPPKFDCSDDMANLTYLGDACVLWNSVIRYVNELIYTYSGLFCIAINPYKRFPIYTYRTMELYTGKRRNECPPHIFAIAEGAYQGMMNSGCNQSILITGESGAGKTENTKKVIAYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325305439|ref|XP_023334330.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2631.28 bits (6819), Expect = 0.000e+0 Identity = 1269/1765 (71.90%), Postives = 1518/1765 (86.01%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+ECPPHIF +AEG+YQGM+N G NQSILITGESGAGKTENTKKVISYFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADMV+YLLEKSRLT+Q LERCYHAFYNLMSD VPDLK+KCLL+D+I DYW+VSQGK+SVESI+DKEDMQ+A +AF ILGF+++E Y+V+K T+ +MHMGNMTKDFVPVGK+EQAEIK E NA VA + GID+EWMI YFCKPKLKVGTEWV KG +C+ A+SSVAGIAR IYE FR + +KCNETL DPTMKKVQYIG LDIAGFEIFD+NGFEQICIN+ NEKLQQFFNQHMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLA+ EEESLFPKATDQ+F KLH NLL K PNF K +P+PDP AHFAV+HYAATVSYNLTSWLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLEA KD GG +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GL+MHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA VAKAK DK AA AVL ++LE EK+RLGHTKVFFRAGILG+MEEVREDR+G+VL+WLQS ARGK+SRM FKK+QDQKLALY CQRTIRNYYIGKTWLWWQ+W+ IKPNLKCT+F +YKAEYE KIA+AE NI KA+A+ KV H+ LL +K+EL LAL SGG+AVQDIIDK R+E D+QKQ+D+ N RI E++ + Q KV + ++LR+EI +E + E+DK+ KD QIRTL++E+ HQ ++I KL +EK+ + +QK EED+Q++ED+ +HL+KVK KLEQSLDE ED LE EKK K D EK+KRK+E DLKLTQE SDLER+ AEL+Q VQRKEKE A++ AKI+DE TLGGKYSKQ KELQSR++ELDEE+++ER +RAKAEK+RS+L +DLED+G+RL EAG+NT+TQ+ELNKKRE ELH++KA++EE NI HEGTLAALR KHNN+MSELGEQID++N NK K+EKDK MERDLQEAR+ LE+ +R +A +++N KM Q I ++N KLDE+ARALNEA+S KK+L +E DL RQI+E ENA+A K KISLTTQLEDTKRLAD EARDR++LLTKYKNL+T+ E+ R +I+ E E+K+D L+ALSKAQAEIQLW+S+FETEG+GR+EELEG++ KL AR+ EAEE +E+LN+KI S+EKS+ R++ +LEEM++EYERTHAAA+I+EKRGRNFD+V+GEWKAKADD+ E+EAS+ ECRN+N+E FRL+AA +E+ EQLD+V+RENKNLADEI+DLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAE ALE EENKVLR+QLELGQVRQEIDR+I EKEEEFNN+RKNHQRAM+S+ ASLE+EQRAK+EALRIKKKLE DINELEIALD +NKAN E QK++KRYQ QLR++ YE+ SR RQE+ E +A+R+ NAL GE+EE+R+LL S+ER KRQ +AEL E+R +VNEM++IN+RA EKR E H +QAEIDDM+ QAKNSEEK+K+AMVDAARLADELR+EQ+H ++ + KRALE+Q+ ELE LA+A +A + GR AM+KLE R+RELE+ELG++Q RT ++AKA+Q+AER +KELQFQ +ED KNQERM++LA KLQ KIKTYK+QIEEAEEIAALNLAKFRKSQQE EE+E+R + A+
Sbjct: 149 RRNECPPHIFAIAEGAYQGMLNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNAAGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNLMSDQVPDLKEKCLLTDDIRDYWYVSQGKLSVESINDKEDMQFADEAFDILGFSQDEKYDVFKNTACMMHMGNMTKDFVPVGKDEQAEIKDEKNANIVATLMGIDAEWMITYFCKPKLKVGTEWVQKGSSCNNAASSVAGIARAIYERTFRLVVEKCNETLCDPTMKKVQYIGVLDIAGFEIFDFNGFEQICINYVNEKLQQFFNQHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAVFEEESLFPKATDQTFCEKLHANLLGKWPNFAKPNPRPDPDAHFAVLHYAATVSYNLTSWLEKNKDPLNDTIVEMIKNGSNSLMIQCFLDHPGQPLEAPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLIMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKLVAKAKNDKGAAGAVLEAIQLEKEKYRLGHTKVFFRAGILGFMEEVREDRIGQVLAWLQSGARGKSSRMQFKKLQDQKLALYCCQRTIRNYYIGKTWLWWQMWMAIKPNLKCTQFGKYKAEYEGKIALAEANIDKAIAECNKVVAEHDRLLNEKNELDLALNSGGNAVQDIIDKTNRIEAARNDLQKQVDQTNARIRGEEETIAGIQQSGIKVTGEATKLRDEIKNLENTIGKCEEDKMTKDNQIRTLRDEIAHQEDLIAKLSKEKKSTGEGRQKIEEDIQSMEDRCNHLNKVKGKLEQSLDECEDTLEREKKAKGDVEKMKRKVEGDLKLTQEATSDLERINAELAQTVQRKEKESASLYAKIEDEQTLGGKYSKQVKELQSRIDELDEEIIVERNNRAKAEKNRSLLSRDLEDIGTRLAEAGSNTSTQIELNKKREAELHKLKADLEEANIAHEGTLAALRQKHNNSMSELGEQIDSINKNKAKSEKDKAGMERDLQEARTGLEEAMRERANMEKNCKMTQSLIVESNQKLDELARALNEADSTKKKLMVESQDLSRQIDETENAIAALQKNKISLTTQLEDTKRLADGEARDRAALLTKYKNLSTDAENLRMKIDEESEKKNDVLRALSKAQAEIQLWRSKFETEGLGRIEELEGSKHKLAARVSEAEEAIESLNSKISSAEKSKHRIESELEEMAMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDIQAELEASHSECRNFNAEGFRLKAALDESNEQLDIVRRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEGALESEENKVLRAQLELGQVRQEIDRRIAEKEEEFNNSRKNHQRAMESMQASLESEQRAKSEALRIKKKLEGDINELEIALDHSNKANNEAQKSIKRYQCQLRESECAYEEASRVRQEMSEKASLAERRANALQGEMEEARSLLDSAERGKRQTEAELAEARNAVNEMTTINSRASAEKRHIEGNCHTMQAEIDDMLHQAKNSEEKSKKAMVDAARLADELRAEQDHVNTQAKAKRALETQMNELEQNLAEANEHAMRGGRTAMAKLETRVRELEIELGNVQGRTGDNAKAHQKAERKIKELQFQNDEDHKNQERMSDLASKLQAKIKTYKKQIEEAEEIAALNLAKFRKSQQELEETEERVRMAD 1912 HSP 2 Score: 479.559 bits (1233), Expect = 9.333e-135 Identity = 210/263 (79.85%), Postives = 233/263 (88.59%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKK+ GPDPDPSPWL+VSPELK K K KPYDPKKS WV +KA GGY EGL++S DG+K TV + + KVFK+DQV QVNPPKFDCSDDM+ LTYL DACVLWNS +RY N+LIYTYSGLFCIAINPYKRFPIYT R+++LY GKRR+ECPPHIF +AEG+YQGM+N G NQSILITGESGAGKTENTKKVISYFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADM
Sbjct: 1 MPGHIKKTTGPDPDPSPWLVVSPELKDKNKLKPYDPKKSVWVKNKADGGYLEGLLESKDGNKWTVNV--KGEMKVFKEDQVCQVNPPKFDCSDDMANLTYLGDACVLWNSTIRYVNQLIYTYSGLFCIAINPYKRFPIYTLRTMELYTGKRRNECPPHIFAIAEGAYQGMLNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNAAGKLSGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325281011|ref|XP_023325178.1| (myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281013|ref|XP_023325179.1| myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281015|ref|XP_023325180.1| myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281017|ref|XP_023325181.1| myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2404.02 bits (6229), Expect = 0.000e+0 Identity = 1216/1767 (68.82%), Postives = 1473/1767 (83.36%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSED--NAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+E PPHIF +AEG++QGMM GKNQSILITGESGAGKTENTKKVISYFAS+GA+GK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FNQ GKL+GADMVIYLLEKSRLT+Q LERCYH+FYNLMSDAVPDLK KCLLS+N+ DY++VSQGK++V+SIDDKEDMQ+A +AF ILGF +EE Y+VYK T+ +MHMG + K F+ VGKEEQA+IK ED +A V+ + GID+EWMINYFCKPKLKVG EWVTKGQT SQASSSVAGIAR +YE F+FI DKCN+TL DPTMK+VQ+IG LDIAGFEIFDYNGFEQICINFCNEKLQQFFN HMFVLEQEEY++EGIEWA VDFGMDLQKCI+MFEKPMGLLAILEEESLFPKATDQSFAAKLH NLL KCPNF K PKPDP AHFAV HYAA VSYNLTSWLEKNKDPLNDT+VE+ KNG+N L + CF DHPGQP E KD GG +K GG KTVSSFYK QL+DLMKTL+AT+P FIRCVVPNTHK+PGG+E L+MHQ CNGVL GI ICRKGFPN+M+Y DFK+RYNILAA VAKAK DK AA AVL V LE EK+RLGHTKVFFRAG+LG+MEEVREDRVG+VLSWLQS RGK SRM F KMQ QKL+LY CQRTIRNY IGKTWLWWQ+W+ +KPNLKCTKF+QYKAEYEEKIAIAE NI KA+A+ KV++ H L +K+++ +L+SGG V+++ K ++E D++KQ+D++N RI +E+ R S Q K++ +LR+EI ++T + +E+D V K+ QIRTLKEEL HQ E+I KL REK+ +++QKTEE +QA EDK +HLSK+K KLEQSLDE+ED+LE EKK + D+EK+K+K+ESDLKLTQET++DL+R+K EL+ +QRKEKE+A++ AKI+DE TLGGKYSKQ KELQ+R+EELDEEL IER +R KAEK+R+ L +D+EDLG +LE+AG NT TQ+ELNKKRE EL ++KA++EE NI EG LA R KHN +SE+G QID+LN K KAE DK MERDL EA++SL+ +R + +++ GK+ Q I ++N +LD+MARALNEA+S KK+L +E DL+RQIE+ E +A+ K KISLTTQLEDT+RLADAE RDR+ L++K+KNL +ELE+ R RIE E E K + LK LSKAQ+EIQLWKS++ETEG+GR++ELEG + KL R+ EAEET+E+L+ K+ S+EKS+ R+ +LE++ LE+ER +AAAI+++KR +NFD+V+GEWK K DDL+ E+EAS +E RNYNSE+FRLRA EE EQLD VKRENKNLA+EI+DLLDQLG+GGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKV+R+QLEL QVRQEIDR+IQEKEEEF NTRKN+QRA+DS+ AS+EAE RAK EALRIKKKLE DINELEIALD ANK+NAE K +KRYQ Q R+ YE+ESR RQE+ + G+ADRK NAL GE+EESR+LL S+ER K+Q + EL ++R +VN+M++IN RA +EKR ES +H + AEID ++ KNSEEKAK+AMVDA+RLA+ELRSEQ+H +S+ + +RAL++Q+ ELE RL + +A K G+ A++KLE RIRELEMELG+ Q RTSE+ K+YQ++ER +KELQFQ +ED+KNQE+M ELA KLQ KI+TYK+QIEEAEEIAALNLAKFRK+QQE EE+E+RS+ AE
Sbjct: 149 RRNEVPPHIFAIAEGAFQGMMVGGKNQSILITGESGAGKTENTKKVISYFASIGATGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNQSGKLAGADMVIYLLEKSRLTYQQPLERCYHSFYNLMSDAVPDLKAKCLLSNNVRDYFYVSQGKIAVDSIDDKEDMQFADEAFDILGFTKEEKYDVYKNTACLMHMGELAKSFIGVGKEEQADIKEEDQKHAKNVSTLLGIDNEWMINYFCKPKLKVGAEWVTKGQTVSQASSSVAGIARSLYERTFKFIVDKCNDTLCDPTMKRVQFIGVLDIAGFEIFDYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYMKEGIEWAMVDFGMDLQKCIEMFEKPMGLLAILEEESLFPKATDQSFAAKLHANLLGKCPNFAKPDPKPDPEAHFAVCHYAAKVSYNLTSWLEKNKDPLNDTIVEMIKNGTNALCVLCFLDHPGQPAETPKDQGGSKKKGGG-KTVSSFYKSQLEDLMKTLHATEPHFIRCVVPNTHKQPGGIEPALIMHQLTCNGVLEGIRICRKGFPNRMSYPDFKSRYNILAAGLVAKAKNDKAAASAVLETVGLEKEKYRLGHTKVFFRAGVLGHMEEVREDRVGQVLSWLQSYCRGKQSRMNFVKMQGQKLSLYCCQRTIRNYMIGKTWLWWQIWITLKPNLKCTKFAQYKAEYEEKIAIAEANIDKAIAECNKVKSAHAKLDEEKADVKRSLESGGDVVKELSAKIEKLEKSRNDLEKQVDQLNTRIRSEEDTRRSAEQTGSKLKKDTDKLRDEIRDVQTNIEKSEEDVVTKESQIRTLKEELAHQEELIQKLTREKKTAGESRQKTEEGIQAAEDKCNHLSKLKLKLEQSLDESEDSLEREKKARADAEKIKKKVESDLKLTQETVADLDRLKEELNSTIQRKEKEFASVAAKIEDEQTLGGKYSKQIKELQTRIEELDEELAIERNNRNKAEKNRATLSRDIEDLGEKLEDAGNNTQTQIELNKKREAELMKLKADMEESNISFEGILANTRSKHNGVISEMGSQIDDLNKAKAKAEADKARMERDLHEAKTSLDAAIRERQNVEKEGKLTQSKIAESNQRLDDMARALNEADSSKKKLTVEGQDLQRQIEDSEAHIAELGKAKISLTTQLEDTRRLADAETRDRAGLMSKFKNLNSELENLRARIEEEAEHKGEILKQLSKAQSEIQLWKSKYETEGLGRIDELEGNKAKLMMRLQEAEETIESLHTKVASTEKSKHRMDTELEDLQLEFERVNAAAIVADKRAQNFDKVVGEWKMKVDDLSSELEASQRESRNYNSEVFRLRAGWEEITEQLDAVKRENKNLAEEIKDLLDQLGEGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVMRAQLELSQVRQEIDRRIQEKEEEFENTRKNYQRALDSMQASMEAETRAKAEALRIKKKLEADINELEIALDHANKSNAEAHKTIKRYQNQYREAETAYEEESRQRQEIVDKAGLADRKANALLGELEESRSLLDSAERGKKQAELELHDARVAVNDMTTINGRAANEKRQLESAVHTLHAEIDSILQSVKNSEEKAKKAMVDASRLAEELRSEQDHCSSQLKARRALDAQVKELELRLEEVVEHATKGGKNAIAKLEGRIRELEMELGNSQMRTSETLKSYQKSERKIKELQFQNDEDQKNQEKMGELATKLQDKIRTYKKQIEEAEEIAALNLAKFRKAQQELEEAEERSRLAE 1914 HSP 2 Score: 477.248 bits (1227), Expect = 4.564e-134 Identity = 209/263 (79.47%), Postives = 236/263 (89.73%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKK GPDPDPS WL VS ELK K K+KPYDPKKSCWVP+KA+GG+ EG+I+ST+GDK+TV++ +KKVFKKD VGQVNPPKFDC +DMS LTYLNDA VLWN +RY NELIYTYSGLFCIA+NPYKRFPIYTQR++++Y+GKRR+E PPHIF +AEG++QGMM GKNQSILITGESGAGKTENTKKVISYFAS+GA+GK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FNQ GKL+GADM
Sbjct: 1 MPGHIKKGSGPDPDPSQWLFVSYELKEKNKAKPYDPKKSCWVPEKATGGFAEGMIESTEGDKITVSV--KGEKKVFKKDLVGQVNPPKFDCCEDMSNLTYLNDASVLWNLRIRYVNELIYTYSGLFCIAVNPYKRFPIYTQRAMEVYMGKRRNEVPPHIFAIAEGAFQGMMVGGKNQSILITGESGAGKTENTKKVISYFASIGATGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNQSGKLAGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022994|ref|XP_023023736.1| (myosin heavy chain, muscle isoform X48 [Leptinotarsa decemlineata]) HSP 1 Score: 1898.63 bits (4917), Expect = 0.000e+0 Identity = 992/1789 (55.45%), Postives = 1339/1789 (74.85%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+ + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 1.987e-94 Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022910|ref|XP_023023693.1| (myosin heavy chain, muscle isoform X6 [Leptinotarsa decemlineata]) HSP 1 Score: 1896.32 bits (4911), Expect = 0.000e+0 Identity = 992/1789 (55.45%), Postives = 1334/1789 (74.57%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL A V+K K + +L+ L++E +RLGHTKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVSKESDPKKCGQIILDASGLDSELYRLGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 352.829 bits (904), Expect = 3.003e-94 Identity = 166/259 (64.09%), Postives = 199/259 (76.83%), Query Frame = 0
Query: 9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 8 EGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022930|ref|XP_023023703.1| (myosin heavy chain, muscle isoform X16 [Leptinotarsa decemlineata]) HSP 1 Score: 1895.94 bits (4910), Expect = 0.000e+0 Identity = 991/1789 (55.39%), Postives = 1337/1789 (74.73%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+ + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 2.443e-94 Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022932|ref|XP_023023704.1| (myosin heavy chain, muscle isoform X17 [Leptinotarsa decemlineata]) HSP 1 Score: 1892.09 bits (4900), Expect = 0.000e+0 Identity = 996/1789 (55.67%), Postives = 1341/1789 (74.96%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY ILA A +AK K+ K+AA+ L + L+A+ +R+GHTKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYMILAPATMAKEKEAKEAARKCLEEIGLDADSYRVGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 347.821 bits (891), Expect = 9.270e-93 Identity = 163/261 (62.45%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKK+ V QVNPPK++ +DM+ LTYLNDA VL N RY ++LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKHFKKENVHQVNPPKYEKVEDMADLTYLNDASVLHNLKQRYYSKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022920|ref|XP_023023698.1| (myosin heavy chain, muscle isoform X11 [Leptinotarsa decemlineata]) HSP 1 Score: 1890.93 bits (4897), Expect = 0.000e+0 Identity = 996/1789 (55.67%), Postives = 1342/1789 (75.01%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K+KCLLS++IYDY +VSQGKV++ ++DD E+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY ILA A +AK K+ K+AA+ L + L+A+ +R+GHTKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKEKCLLSNDIYDYNYVSQGKVTIPNVDDGEECALTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYMILAPATMAKEKEAKEAARKCLEEIGLDADSYRVGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 2.184e-94 Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022924|ref|XP_023023700.1| (myosin heavy chain, muscle isoform X13 [Leptinotarsa decemlineata]) HSP 1 Score: 1887.46 bits (4888), Expect = 0.000e+0 Identity = 988/1789 (55.23%), Postives = 1334/1789 (74.57%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+ + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K K+EK+K +L + R+S++ KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKSEKEKAQYFGELNDLRASVDHLANEKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 2.057e-94 Identity = 166/259 (64.09%), Postives = 199/259 (76.83%), Query Frame = 0
Query: 9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 8 EGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264 The following BLAST results are available for this feature:
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of myosin heavy muscle isoform x20 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold286_size222086:148956..164589+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold286_size222086-processed-gene-1.7 ID=snap_masked-scaffold286_size222086-processed-gene-1.7|Name=myosin heavy muscle isoform x20|organism=Tigriopus kingsejongensis|type=gene|length=15634bp|location=Sequence derived from alignment at scaffold286_size222086:148956..164589+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'myosin heavy muscle isoform x20' has the following synonyms
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