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The key to sustainable sea lice control is in the genome.

LsRXR

Michael Dondrup's picture
General
End Date: 
Tuesday, April 7, 2015 - 10:02
Experiment Batch ID: 
LsRXR
Start Date: 
Friday, March 13, 2015 - 11:54
Sample
Developmental Stage CV: 
Adult
Copepodid
Sex: 
female
Number of Individuals (Start): 
50
Target description
Gene or target symbol: 
LsRXR
Target type: 
protein_coding_primary_transcript
Fragment description
Fragment Producer Contact: 
Fragment Label ID: 
RNAiFr1
Fragment length: 
301bp
Fragment Producer: 
licelab
Validation
Efficacy of Knock Down: 
F>60.00%
Phenotype
Number of Individuals (End): 
38
Gallery Image: 
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Background

Nuclear receptors have crucial roles in all metazoan animals as regulators of gene transcription. A wide range of studies have elucidated molecular and biological significance of nuclear receptors but there are still a large number of animals where the knowledge is very limited. In the present study we have identified an RXR type of nuclear receptor in the salmon louse (Lepeophtheirus salmonis) (i.e. LsRXR). RXR is one of the two partners of the Ecdysteroid receptor in arthropods, the receptor for the main molting hormone 20-hydroxyecdysone (E20) with a wide array of effects in arthropods.

Results

Five different LsRXR transcripts were identified by RACE showing large differences in domain structure. The largest isoforms contained complete DNA binding domain (DBD) and ligand binding domain (LBD), whereas some variants had incomplete or no DBD. LsRXR is transcribed in several tissues in the salmon louse including ovary, subcuticular tissue, intestine and glands. By using Q-PCR it is evident that the LsRXR mRNA levels vary throughout the L. salmonis life cycle. We also show that the truncated LsRXR transcript comprise about 50% in all examined samples. We used RNAi to knock-down the transcription in adult reproducing female lice. This resulted in close to zero viable offspring. We also assessed the LsRXR RNAi effects using a L. salmonis microarray and saw significant effects on transcription in the female lice. Transcription of the major yolk proteins was strongly reduced by knock-down of LsRXR. Genes involved in lipid metabolism and transport were also down regulated. Furthermore, different types of growth processes were up regulated and many cuticle proteins were present in this group.

Conclusions

The present study demonstrates the significance of LsRXR in adult female L. salmonis and discusses the functional aspects in relation to other arthropods. LsRXR has a unique structure that should be elucidated in the future.