Rhabdovirus - No9+127 Specificity

Submitted by Aina-Cathrine Ø... on 19 April, 2018 - 15:00

General

Experiment Description:

To analyze the specificity of the knock-down, nauplius I from three females positive for both viruses were each divided into five groups. One group were not added any dsRNA (untreated) while the rest of the groups were added control dsRNA, N protein dsRNA from the No9 strain, N protein dsRNA from the No127 strain, or N protein dsRNA from both No9 and No127 strain. Samples were taken at 0, 4 and 8 days after soaking for analyzing the viral N protein RNA level. Reference: see Øvergård et al 2017; RNAi-mediated treatment of two vertically transmitted rhabdovirus infecting the salmon louse (Lepeophtheirus salmonis).

Experiment Batch ID:

Exp2

Start Date:

Sunday, April 19, 2015 - 14:11

Experiment Contact:

Aina-Cathrine Øvergård

Frank Nilsen

Publication:

RNAi-mediated treatment of two vertically transmitted rhabdovirus infecting the salmon louse (Lepeophtheirus salmonis)

Sample

Developmental Stage CV:

Nauplius I

Copepodid

Number of Individuals (Start):

540

Sex:

unknown

Organism:

Lepeophtheirus salmonis

Target description

Gene or target symbol:

N protein LsRV-No9

N protein LsRV-No127

Target type:

Viral

Primary target ID:

LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9

LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127

Fragment description

Fragment Producer Contact:

Aina-Cathrine Øvergård

Fragment Label ID:

FNo9

FNo127

Fragment concentration:

3000.00ng/ul

Fragment length:

896bp

Sense sequence:

No9: ttctcccggaccgacatggaaacagagtttcatgttaatctatccaagcccggtattcaatcacaatatccatctgagtggttttctgctggaaacaaaaggcccagtgtgtccgtgaaggtcccaacaacgtatctgaaaaaggagtcaagagattacttttgtaatctcctaaacaactcattgacagtccccgaatggtcaacgatccaagggatggtctggctctcggagatgtatcctagtcaactgaaggggaatgatgtactagagtcattcgggataaagttaactgatgataaaaaccaggtgacggtcaaatcaattcttaactatgccgaggtagaggatcccactactacactcaaacccccagccaacaacaaaacagaatggactgaccaggagtactttgtcgggattacggcagtactggtcagttaccggtatcacagacttgtgcacgaggaggggagggaggccttactgagaagattagccggaatgctgacatctcaaacgctaagccttgcgtctattgttcggaaagccctcccagaacccggcgaagacatgaggaaggttatggcagcgctagatctccagttcaaccttggagatgggaaccccgaattcaaaagagtcaggatggggacagttcatctgcttcagagggatcagacatttctaagtgaagttcttgccatatctggttggtttgggaccgagtcctatgaaatgatcaaatggattttctctgccgaggtagcgaaggaggtcagagatagattttctcatgcagacgaggaagcaggacaacaattttcttactctgcctatatgattgatttcgggatttccaacagatctccttactcagtcaccgccaatccctt No127: ggagccatcggaggttatgacctgggtgactcttgattccgtctcgactgagatcttgaagaccgtcagagatactgaggaaatttattgtcctttctcttatgccccctatatgatggatctcgggatatcagctaagtctccgtattcggtcacagaaaatcctttgtctcacctatggattcatgtgacaggagtaatattaaataacccgcgatctatgaatgcccggattatcggggagaccacatttcatcaggtaattttgtcctccgccctcctggcttatcggtgcgcaacaagtcctttattgagtcaaaagttcagtggacaatccgacctcagaggggtagaagagagtattagggtcttgtcggagtcaagttcatatttctcaaacactgaggatcttgatctggaagacgatccacaacaaaacactcccatggcatacgtcgcttggtgtgacaacaagtggaaccgtcgactttttacatttgtagactccgcaatgctaggattgacacggcccctt

Fragment Mixture Formula:

FNo9+FNo127

Fragment Producer:

Aina-Cathrine Øvergård

Validation

Efficacy of Knock Down:

F>94.00%

Phenotype

Number of Individuals (End):

540

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