Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance

Overview
TitleTranscriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance
AuthorsSutherland BJ, Poley JD, Igboeli OO, Jantzen JR, Fast MD, Koop BF, Jones SR
TypeJournal Article
Journal NameEvolutionary applications
Volume8
Issue2
Year2015
Page(s)133-48
CitationSutherland BJ, Poley JD, Igboeli OO, Jantzen JR, Fast MD, Koop BF, Jones SR. Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance. Evolutionary applications. 2015 Feb; 8(2):133-48.

Abstract

Salmon lice Lepeophtheirus salmonis are an ecologically and economically important parasite of wild and farmed salmon. In Scotland, Norway, and Eastern Canada, L. salmonis have developed resistance to emamectin benzoate (EMB), one of the few parasiticides available for salmon lice. Drug resistance mechanisms can be complex, potentially differing among populations and involving multiple genes with additive effects (i.e., polygenic resistance). Indicators of resistance development may enable early detection and countermeasures to avoid the spread of resistance. Here, we collect sensitive Pacific L. salmonis and sensitive and resistant Atlantic L. salmonis from salmon farms, propagate in laboratory (F1), expose to EMB in bioassays, and evaluate either baseline (Atlantic only) or induced transcriptomic differences between populations. In all populations, induced responses were minor and a cellular stress response was not identified. Pacific lice did not upregulate any genes in response to EMB, but downregulated degradative enzymes and transport proteins at 50 ppb EMB. Baseline differences between sensitive and now resistant Atlantic lice were much greater than responses to exposures. All resistant lice overexpressed degradative enzymes, and resistant males, the most resistant group, overexpressed collagenases to the greatest extent. These results indicate an accumulation of baseline expression differences related to resistance.

Author Details
Additional information about authors:
Details
1Ben J G Sutherland
2Jordan D Poley
3Okechukwu O Igboeli
4Johanna R Jantzen
5Mark D Fast
6Ben F Koop
7Simon R M Jones
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1752-4571
eISSN1752-4571
Publication Date2015 Feb
Journal AbbreviationEvol Appl
DOI10.1111/eva.12237
Elocation10.1111/eva.12237
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PMID:25685190