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Molecular characterization and functional analysis of a salmon louse (Lepeophtheirus salmonis, Krøyer 1838) heme peroxidase with a potential role in extracellular matrixes

Overview
TitleMolecular characterization and functional analysis of a salmon louse (Lepeophtheirus salmonis, Krøyer 1838) heme peroxidase with a potential role in extracellular matrixes
AuthorsØvergård AC, Eichner C, Nilsen F, Dalvin S
TypeJournal Article
Journal NameComparative biochemistry and physiology. Part A, Molecular & integrative physiology
VolumeN/A
IssueN/A
Year2017
Page(s)N/A
CitationØvergård AC, Eichner C, Nilsen F, Dalvin S. Molecular characterization and functional analysis of a salmon louse (Lepeophtheirus salmonis, Krøyer 1838) heme peroxidase with a potential role in extracellular matrixes. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 2017 Jan 10.

Abstract

<p>Heme peroxidases are the most abundant type of peroxidase catalyzing a H2O2-dependent oxidation of a wide variety of substrates. They are involved in numerous processes like the innate immune response, hormone and prostaglandin synthesis and crosslinking of proteins within extracellular matrixes (ECM) as well as molecules within the cuticle and chorion of arthropods and nematodes. In the present study, a Lepeophtheirus salmonis heme peroxidase (LsHPX) 1 was characterized. Amino acids in the active site of heme peroxidases were conserved, and the predicted protein sequence showed the highest similarity to genes annotated as chorion peroxidases and genes suggested to be involved in cuticle hardening or adhesion. LsHPX1 exhibited a dynamic expression during ontogenesis and during the nauplius molting cycle. Transcripts were localized to muscle cells near the muscle-tendon junction, in nerve tissue especially at neuromuscular junctions, subcuticular epithelium, subepithelial cells facing the hemolymph, exocrine glands within the subepithelial tissue and in isolated cells within the testis. Knock-down of LsHPX1 in nauplius larvae decreased the swimming activity of emerging copepodids. Histological analysis of knock-down animals revealed increased spacing between myofibers and changes in subepithelial and exocrine gland tissue. Considering these results, the potential role of LsHPX1 in crosslinking molecules of salmon louse ECMs is discussed.</p>
Author Details
Additional information about authors:
Details
1Aina-Cathrine Øvergård
2Christiane Eichner
3Frank Nilsen
4Sussie Dalvin
Properties
Additional details for this publication include:
Property NameValue
Journal CountryUnited States
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
CopyrightCopyright © 2017. Published by Elsevier Inc.
DOI10.1016/j.cbpa.2017.01.004
Elocation10.1016/j.cbpa.2017.01.004
PIIS1095-6433(17)30004-1
Journal AbbreviationComp. Biochem. Physiol., Part A Mol. Integr. Physiol.
Publication Date2017 Jan 10
eISSN1531-4332
ISSN1531-4332
Publication ModelPrint-Electronic
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DatabaseAccession
PMID: PubMedPMID:28087330