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Characterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands

Overview
TitleCharacterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands
AuthorsHarasimczuk E, Øvergård AC, Grotmol S, Nilsen F, Dalvin S
TypeJournal Article
Journal NameMolecular and biochemical parasitology
Volume219
IssueN/A
Year2018
Page(s)1-9
CitationHarasimczuk E, Øvergård AC, Grotmol S, Nilsen F, Dalvin S. Characterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands. Molecular and biochemical parasitology. 2018 Jan; 219:1-9.

Abstract

<p>The salmon louse, Lepeophtheirus salmonis (Copepoda: Caligidae), is currently the most significant pathogen affecting the salmon farming industry in the Northern Hemisphere. Exocrine glands of blood-feeding parasites are believed to be important for the host-parasite interaction, but also in the production of substances for integument lubrication and antifouling. In L. salmonis; however, we have limited knowledge about the exocrine glands. The aim of this study was therefore to examine three genes containing fibronectin type II (FNII) domains expressed in L. salmonis tegumental type 1 (teg 1) glands, namely LsFNII1, 2 and 3. LsFNII1, 2 and 3 contains four, three, and two FNII domains respectively. Sequence alignment of LsFNII domains showed conservation of amino acids that may indicate a possible involvement of LsFNII domains in collagen binding. Ontogenetic analysis of LsFNII1, 2 and 3 revealed highest expression in pre-adult and adult lice. Localization of LsFNII1, 2 and 3 transcripts showed expression in teg 1 glands only, which are the most abundant exocrine gland type in L. salmonis. LsFNII1, 2 and 3 were successfully knocked-down by RNAi, however, alteration in gland morphology was not detected between the knock-down and control groups. Overall, this study gives first insight into FNII domain containing proteins in L. salmonis.</p>
Author Details
Additional information about authors:
Details
1Ewa Harasimczuk
2Aina-Cathrine Øvergård
3Sindre Grotmol
4Frank Nilsen
5Sussie Dalvin
Properties
Additional details for this publication include:
Property NameValue
Journal CountryNetherlands
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
CopyrightCopyright © 2017 Elsevier B.V. All rights reserved.
DOI10.1016/j.molbiopara.2017.12.002
Elocation10.1016/j.molbiopara.2017.12.002
PIIS0166-6851(17)30153-6
Journal AbbreviationMol. Biochem. Parasitol.
Publication Date2018 Jan
eISSN1872-9428
ISSN1872-9428
Publication ModelPrint-Electronic
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PMID: PubMedPMID:29246806