Identification and characterisation of the ecdysone biosynthetic genes neverland, disembodied and shade in the salmon louse Lepeophtheirus salmonis (Copepoda, Caligidae)

Overview
TitleIdentification and characterisation of the ecdysone biosynthetic genes neverland, disembodied and shade in the salmon louse Lepeophtheirus salmonis (Copepoda, Caligidae)
AuthorsSandlund L, Kongshaug H, Horsberg TE, Male R, Nilsen F, Dalvin S
TypeJournal Article
Journal NamePloS one
Volume13
Issue2
Year2018
Page(s)e0191995
CitationSandlund L, Kongshaug H, Horsberg TE, Male R, Nilsen F, Dalvin S. Identification and characterisation of the ecdysone biosynthetic genes neverland, disembodied and shade in the salmon louse Lepeophtheirus salmonis (Copepoda, Caligidae). PloS one. 2018; 13(2):e0191995.

Abstract

The salmon louse is a marine ectoparasitic copepod on salmonid fishes. Its lifecycle consists of eight developmental stages, each separated by a molt. In crustaceans and insects, molting and reproduction is controlled by circulating steroid hormones such as 20-hydroxyecdysone. Steroid hormones are synthesized from cholesterol through catalytic reactions involving a 7,8-dehydrogenase Neverland and several cytochrome P450 genes collectively called the Halloween genes. In this study, we have isolated and identified orthologs of neverland, disembodied and shade in the salmon louse (Lepeophtheirus salmonis) genome. Tissue-specific expression analysis show that the genes are expressed in intestine and reproductive tissue. In addition, levels of the steroid hormones ecdysone, 20-hydroxyecdysone and ponasterone A were measured during the reproductive stage of adult females and in early life stages.

Author Details
Additional information about authors:
Details
1Liv Sandlund
2Heidi Kongshaug
3Tor Einar Horsberg
4Rune Male
5Frank Nilsen
6Sussie Dalvin
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1932-6203
eISSN1932-6203
Publication Date2018
Journal AbbreviationPLoS ONE
DOI10.1371/journal.pone.0191995
Elocation10.1371/journal.pone.0191995
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
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PMID: PubMedPMID:29401467