AV F404

Submitted by Andreas Borchel on 2 May, 2019 - 09:49

Phenotype CV:

General

Experiment Batch ID:

AV

Experiment Contact:

Andreas Borchel

Start Date:

14 March, 2019 - 08:35

End Date:

24 April, 2019 - 08:23

Experiment Description:

injection into pradult females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult1

Adult

Target description

Primary target ID:

EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment length:

400bp

Fragment concentration:

600ng/ul

Sense sequence:

TGTTGGCCCTATGAGTCCATCT TTTTTGTGACTTTGATGCCTTGA

Antisense sequence:

TGATCCTTGGAAGAAAAGAGGATG TTCATCATTTCCAGCATTGAGTTT

Fragment Label ID:

F404

Fragment Mixture Formula:

50% of both, in total 600 ng/ul

Fragment Producer:

licelab

Fragment Producer Contact:

Andreas Borchel

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

15

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Knockdown of a testis-specific ion-pump in pre-adult1 males, checking reproduction in adults. Many males were lost, and no kncokdown observed.

AS F384

Submitted by Andreas Borchel on 21 November, 2018 - 11:10

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002328, EMLSAT00000002328-698175 (mRNA) Lepeophtheirus salmonis

EMLSAT00000011195, EMLSAT00000011195-707042 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010070, EMLSAT00000010070-705917 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment concentration:

600ng/ul

Fragment Batch ID:

F384

Fragment Label ID:

F384

Fragment Mixture Formula:

F381+F382+F383 in same ratio

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

21

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knockdown lead to missing egg strings

AS F380

Submitted by Andreas Borchel on 21 November, 2018 - 09:59

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000000454, EMLSAT00000000454-696301 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAT00000000454

Fragment description

Fragment length:

500bp

Fragment concentration:

600ng/ul

Sense sequence:

GTCCACTCGTATGCCCACCTCTCTGTGAGGAACATGAAATGTTTTGTCCCGGATCCATGGTTGATGAATGTCCTACAGAAGATAATTGCGTTGCCAAAATTGATGAAAATAACAAGTGTCCAAATTATTGTCCAATTACTTGTGAAAAAGGTCAACACATATGTCCTGGAATCCGAAAAGATGATGGATGCAGAGGTCCAGATACCTGTGTACCCATTGGTACACAATGTCCAAGTGAGATTTTTGATGAATTTGCTTGTCCCATCATTGATTCACCCAGTTGTGTGTATCCCGAAACTCATTGCCATGGTGGTTACAATTCAAGGGGTTGTCGAAAACGATCCTTCTGTAAGGAATTAAAGAATAAAACAGAGCATCAACATGGTGTAAATGGCATTGATGGTTGTCCAACATTTTGTCCAGCAACATGCATTCCAGGTATTGAGACAGAATGCCCTGAAACCTACGATGAAGATGGATGTCCTCTTCCTGCGTCTTGC

Fragment Batch ID:

F380

Fragment Label ID:

F380

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

20

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knockdown lead to missing egg strings

AL F339

Submitted by Joakim Brunet on 14 June, 2018 - 13:14

Phenotype CV:

General

Experiment Batch ID:

RNA_AL

Experiment Contact:

Joakim Brunet

Start Date:

28 August, 2017 - 10:00

End Date:

6 October, 2017 - 00:00

Experiment Description:

Knockdown of the nuclear receptor FTZF1

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis

EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis

Gene or target symbol:

FTZ-F1

Target type:

mRNA

Fragment description

Fragment length:

499bp

Sense sequence:

gtgatagggcacggaaactgcaagtcttgcgt Caaagacaagtgatacacggtggagtttccatgggcccaagtagcatgaataatacgaca Accacaacgagtagtagtgcatcctacggcggcggtgcctccggtggaatgaactccacg Agtagccctccatccatgaacaataataataataacaattccccctcaatctacggctca Cccatcactcatattaaacaagagatacaaataccacaagtcacatctatgacgtcttca Ccagatacttcaccctctcccgttcatttacccggaatgatttctaatcctggaggcaat Ccaatgggtggagatactccgggctccaatcccatcattgcccaacagactgatcctgcc Ggaggtattcaatggcagatcaatgctgctggaaagcatcaggatatccaaagtaatcaa Ggaactgggaaaatgccagccatcattcgagaattccttgcctccct

Fragment ID:

FTZF1 DsRNA probe 2, FTZF1 DsRNA 2 (RNAi_reagent) Lepeophtheirus salmonis

Fragment Batch ID:

F339

Fragment Label ID:

F339

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>45%

Phenotype

Number of Individuals (End):

7

Phenotype:

No eggstrings

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RFAL14.jpg

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Knockdown of FTZF1 targetting both isoforms, alpha and beta. Only alpha is downregulated, few survivors. Sectioning reveals disorganization of eggs in genital segment, resulting in no eggstrings, same as probe F338 (other entry).

AL F338

Submitted by Joakim Brunet on 14 June, 2018 - 12:56

Phenotype CV:

General

Experiment Batch ID:

RNA_AL

Experiment Contact:

Joakim Brunet

Start Date:

28 August, 2017 - 10:00

End Date:

6 October, 2017 - 10:00

Experiment Description:

Knockdown of the nuclear receptor Fushi tarazu factor 1 (FTZF1)

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis

EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis

Gene or target symbol:

FTZ-F1

Target type:

mRNA

Fragment description

Fragment length:

467bp

Sense sequence:

cgccttcatcatcgacaacaacatccatactaacctcatctacaaataaaaaacctcgttccctcaaaatcgtcgaatgtctcgactct tcctcgacgacacacaccacagcctctccatcgtcgccatcaacctcttcatggaatctc Attcttccttcgcatccttccgtagaatcaccttcatcgtcttcgagtccttctcatcaa Ggagggggagataaagagatatcctctgtggttatagcagcctctacgaccacgacagca Actaaaagatcatttagtcaacatatcatggaagaagaggaagaggacgaagacgaagaa Gaggctggaggacacttatcttggaaccctgatgaagagcaggatcaatcagctgaggag Gatgcaggactaccaacagcatccagtaacaacaactccgctggatccacatccggagacttcggtcctggagttgtg

Fragment ID:

FTZF1 DsRNA probe 1, FTZF1 DsRNA 1 (RNAi_reagent) Lepeophtheirus salmonis

Fragment Batch ID:

F338

Fragment Label ID:

F338

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>45%

Phenotype

Number of Individuals (End):

12

Phenotype:

No eggstrings

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RFAL12.jpg

RFAL11_s123_1_genitalsegment_25x.jpg

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Knockdown of the nuclear FTZ-F1 in pre-adult 2. Knockdown resulted in strange genital segments. Sectioning revealed disorganization of eggs in genital segment.

AL Hormone receptor 3 (HR3)

Submitted by Joakim Brunet on 14 June, 2018 - 10:47

Phenotype CV:

General

Experiment Batch ID:

RNA_AL

Experiment Contact:

Joakim Brunet

Start Date:

28 August, 2017 - 10:00

End Date:

6 October, 2017 - 10:00

Experiment Description:

Knockdown of the ortholog of the nuclear receptor HR3. Injection of pre-adult 2 females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000000733

Target type:

mRNA

Fragment description

Fragment length:

441bp

Sense sequence:

gccta Gcaccattcgagcaagaaatctcattgcaaaacaaacaaacaaaaaggattcatagaata Aatagaaaaaggaccctacaaggcaaaaaaaacagcaactattttttacaaattgtttcg Tactcgtcgatgcaaaatcagaaatattatacgaatttgtcctcaatggattggatatca Ggctatcgtcttccatccaattcggatttaaagtgtgatgatgatcaccatggcggcggg Gcaggagtacagcatgcatctgttgtcggcggaccaacaggcggagcactaggatctggc Tcttccggtcccctcacaaacaacaccaattccaactcttcctcaccctccatgctaggc Atgtgggcagcagctgtggccaatacacctcttaaagtggaaacagctgatcatcatgga Aatggatctctggtgg

Fragment Batch ID:

F341

Fragment Label ID:

F341

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Number of Individuals (End):

17

Phenotype:

No eggstrings. Disorganization of eggs in genital segment

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RFAL16_HR3_s109_s2_genital_2.5x_AX10_ScopeA1.jpeg

RAL72.jpg

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Knockdown of HR3 in pre-adult 2 females. Resulted in no eggstring production. Sections reveals disorganized eggs in genital segment. Ovaries look normal.

AQ F375 EMLSAG00000008959

Submitted by Christiane Eichner on 29 May, 2018 - 14:58

Phenotype CV:

General

Experiment Batch ID:

RAQ

Experiment Contact:

Christiane Eichner

Start Date:

16 April, 2018 - 09:00

End Date:

23 May, 2018 - 09:00

Experiment Description:

Knock-down of EMLSAG00000008959

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000008959

Fragment description

Fragment length:

432bp

Fragment concentration:

600ng/ul

Sense sequence:

gcctccggttcggatgaagaagtagaagaggaagaaataactgaatatgatgccttgttaaaggtgtatggagctcaaacatatgttgatgaagctgaagaggaagagatatgcgaaggagaaatggaggaggaagaaaacgatatggatgaggaagatgtatcattgaatggagtagatgggatagatgaagaagatttattgtcgaatgaagaagaaaatgaaactatcgatctcgaaagtggagaagatgaaaatgaaactatcgatctcgaaagtggagaagaggaaaatgaaactatcgatctcgaaagtggagaagaagaagaagagattgtggatactgatatcggaagkgrtatattagatgaaaatgaggatgaatttccagagatggatgacgaaaawgacacttgtggcccctctgatcct

Fragment Label ID:

F375

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

5

Phenotype:

no eggstreng

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Knock-down of EMLSAT00000003179. No visible phenotype

AP F366 EMLSAT00000001458

Submitted by Christiane Eichner on 29 May, 2018 - 11:26

Phenotype CV:

General

Experiment Batch ID:

AP

Experiment Contact:

Christiane Eichner

Start Date:

6 March, 2018 - 09:00

End Date:

10 April, 2018 - 09:00

Experiment Description:

EMLSAG1458 knock-down

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000001458

Fragment description

Fragment length:

412bp

Fragment concentration:

600ng/ul

Sense sequence:

>F366 caagctgttattgattggcgattctggtgtaggcaaatcgtgcttactattaagatttgctgatgatacttacacagaaagttacataagtacaattggtgttgactttaagattagaacaattgaactcgacggaaagactatcaagttgcaaatctgggatactgcaggccaagaaagattcagaactatcacatccagttactatcgtggtgcccacggaatcatcgttgtatatgacgttacggaccaggattcttttaataaccttaagcaatggcttcaagaaattgatagatacgcatgtgaaaatgtaaacaagcttcttgtgggaaacaaatgtgatctgacaacaaagaaggttgtagattatacaactgcaaaagaatacgctgatcagttaaatatgccg

Fragment Label ID:

F366

Primer Group:

likb2502_T7_b2515

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Eichner

Validation

Efficacy of Knock Down:

F>77%

Phenotype

Number of Individuals (End):

13

Phenotype:

no eggstreng

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Testing the fragment for Mvp1 knock-down.

Mucin-like spermatophore wall protein 2 (AJ F270)

Submitted by Andreas Borchel on 26 March, 2018 - 10:39

Phenotype CV:

General

Experiment Batch ID:

AJ

Experiment Contact:

Andreas Borchel

Start Date:

21 March, 2017 - 09:08

End Date:

25 April, 2017 - 09:08

Experiment Description:

injection into young adut males, checking reproduction & spermatophore attachment of females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

MLSWP2

Fragment description

Fragment length:

497bp

Fragment concentration:

600ng/ul

Sense sequence:

CCAGGCTTTTCACACGCTTT

Antisense sequence:

GCTCTTGGCCGAGCATAAAA

Fragment Batch ID:

F270

Fragment Label ID:

F270

Validation

Efficacy of Knock Down:

F>80%

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Control fragment used for all samples

Mucin-like spermatophore wall protein 1 (AJ F269)

Submitted by Andreas Borchel on 26 March, 2018 - 10:34

Phenotype CV:

General

Experiment Batch ID:

AJ

Experiment Contact:

Andreas Borchel

Start Date:

21 March, 2017 - 09:08

End Date:

25 April, 2017 - 09:08

Experiment Description:

injection into young adult males, checking reproduction & spermatophore attachment of females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAT00000007670, EMLSAT00000007670-703517 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

MLSWP1

Fragment description

Fragment length:

527bp

Fragment concentration:

600ng/ul

Sense sequence:

TTACGTTGGCAATCGTCGTC

Antisense sequence:

GAAATCAAGTGCGGCTCCA

Fragment Batch ID:

F269

Fragment Label ID:

F269

Validation

Efficacy of Knock Down:

F>95%

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Control fragment used for all samples