RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort descending Publication
1 E F50 (Ferr_sub) EMLSAG00000010792, EMLSAG00000010792-693558 (gene) Lepeophtheirus salmonis
ferr_sub
PreAdult2, Adult Most lice didn’t have egg strings at termination (
female reproduction, no eggstrings
38/18 Lepeophtheirus salmonis F>85.00% 21 February, 2013 - 11:54
2 D F29 EMLSAG00000006214, EMLSAG00000006214-688980 (gene) Lepeophtheirus salmonis
g2282_trehalose
PreAdult2 a bit short eggstrings (?), other wise normal.
female reproduction
36/12 Lepeophtheirus salmonis F>59.00% 6 March, 2013 - 14:49
3 E F34 EMLSAG00000003242, EMLSAG00000003242-686008 (gene) Lepeophtheirus salmonis
g7499_LsRaptor
PreAdult2, Adult female reproduction, egg string deformation, no eggstrings 39/24 Lepeophtheirus salmonis F>50.00% 12 March, 2013 - 09:05
Molecular characterization and functional analysis of...
4 E F42 EMLSAT00000005071, EMLSAT00000005071-700918 (mRNA) Lepeophtheirus salmonis
g13677_Rheb
PreAdult2, Adult Low reproduction and most are missing blood in the
feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings
39/25 Lepeophtheirus salmonis F>60.00% 12 March, 2013 - 10:38
Molecular characterization and functional analysis of...
5 H F60 EMLSAG00000003343, EMLSAG00000003343-686109 (gene) Lepeophtheirus salmonis
FK506
PreAdult2, Adult female no reproduction
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>14.00% 9 April, 2013 - 13:26
6 H F59 EMLSAG00000011506, EMLSAG00000011506-694272 (gene) Lepeophtheirus salmonis
g9531
PreAdult2, Adult no visible phenotype 9 Lepeophtheirus salmonis F>0.00% 9 April, 2013 - 13:37
7 K F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua PreAdult2, Adult Control 36/16 Lepeophtheirus salmonis 22 April, 2013 - 14:34
8 K F76 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
UN7_g423, LSalAtl2s685
PreAdult2, Adult No visible phenotype
no visible phenotype
34/14 Lepeophtheirus salmonis 22 April, 2013 - 14:36
9 K F77 EMLSAT00000003661, EMLSAT00000003661-699508 (mRNA) Lepeophtheirus salmonis
UN8_g528
PreAdult2, Adult female reproduction 36/19 Lepeophtheirus salmonis F>90.00% 22 April, 2013 - 14:51
10 K F93 EMLSAG00000009301, EMLSAG00000009301-692067 (gene) Lepeophtheirus salmonis
UN14_g1154
PreAdult2, Adult No visible phenotype
no visible phenotype
33/16 Lepeophtheirus salmonis 22 April, 2013 - 14:53

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