RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
111 AC F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Adult, Adult No phenotype 30/24 Lepeophtheirus salmonis F>99.91% 12 April, 2016 - 10:35
112 AG F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No phenotype
no visible phenotype
30/16 Lepeophtheirus salmonis F>99.90% 21 February, 2017 - 10:15
113 F230 Nauplia EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Nauplius I, chalamus II/padI No phenotype
no visible phenotype
550/240 Lepeophtheirus salmonis F>98.50% 21 February, 2017 - 10:25
114 EMLSAT00000005299 cop + infection EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
Emc3
Nauplius1, chalimus and preadult no proper visible phenotype but maybe quicker deve
no visible phenotype
180/52 Lepeophtheirus salmonis 14 June, 2018 - 14:05
115 EMLSAT00000003179 cop + infection EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis
Csdc2
Nauplius1, Copepodid no proper visible phenotype but maybe quicker deve
no visible phenotype
180/45 Lepeophtheirus salmonis 14 June, 2018 - 13:09
116 H F60 EMLSAG00000003343, EMLSAG00000003343-686109 (gene) Lepeophtheirus salmonis
FK506
PreAdult2, Adult female no reproduction
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>14.00% 9 April, 2013 - 13:26
117 T F169 EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis
UN53_5145, LsMvp1
PreAdult2, Adult normal
no visible phenotype
29/13 Lepeophtheirus salmonis F>67.00% 14 November, 2014 - 13:41
118 AP F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3
PreAdult2 normal
no visible phenotype
30/15 Lepeophtheirus salmonis F>68.00% 13 April, 2018 - 09:45
119 AV F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3
PreAdult2 normal
no visible phenotype
30/15 Lepeophtheirus salmonis 2 May, 2019 - 09:38
120 AV F0 extra CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal
no visible phenotype
30/17 Lepeophtheirus salmonis 2 May, 2019 - 09:59

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