RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
31 Nauplii I 3059-1 EMLSAG00000003059, EMLSAG00000003059-685825 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:54
32 Nauplii I 7798-1 EMLSAG00000007798, EMLSAG00000007798-690564 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:54
33 RNAi_AS 11366+11369+10832 EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:07
34 Heme peroxidase F415 EMLSAT00000013035, EMLSAT00000013035-708882 (mRNA) Lepeophtheirus salmonis, EMLSAT00000013034, EMLSAT00000013034-708881 (mRNA) Lepeophtheirus salmonis
hpx
Adult 35 Lepeophtheirus salmonis 2 May, 2019 - 09:42
35 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:32
36 D F29 EMLSAG00000006214, EMLSAG00000006214-688980 (gene) Lepeophtheirus salmonis
g2282_trehalose
PreAdult2 a bit short eggstrings (?), other wise normal.
female reproduction
36/12 Lepeophtheirus salmonis F>59.00% 6 March, 2013 - 14:49
37 T F166 EMLSAT00000010071, EMLSAT00000010071-705918 (mRNA) Lepeophtheirus salmonis
UN50_10071
PreAdult2, Adult Abnormal
female reproduction, abnormal egg strings
30/19 Lepeophtheirus salmonis F>96.00% 14 November, 2014 - 13:26
38 R F161 EMLSAG00000000580, EMLSAG00000000580-683346 (gene) Lepeophtheirus salmonis
UN38_580
PreAdult2, Adult Abnormal eggstrings in some individuals
female reproduction, abnormal egg strings
37/20 Lepeophtheirus salmonis F>99.00% 7 October, 2014 - 10:17
39 T F178 EMLSAT00000003607, EMLSAT00000003607-699454 (mRNA) Lepeophtheirus salmonis
3607
PreAdult2, Adult A few slightly abnormal 28/14 Lepeophtheirus salmonis F>0.00% 14 November, 2014 - 13:20
40 T F181 EMLSAG00000001530, EMLSAG00000001530-684296 (gene) Lepeophtheirus salmonis PreAdult2 All animals with short and abnormal egg strings, f 30/10 Lepeophtheirus salmonis 26 February, 2015 - 15:51

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