RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
391 ExpH - EMLSAT000000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>95.00% 14 June, 2018 - 11:08
392 EMLSAT00000009839_Var1Frag1 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult survival, reduced 60/5 Lepeophtheirus salmonis 14 June, 2018 - 11:48
393 EMLSAT00000009839_Var1Frag2 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult survival, reduced 60/3 Lepeophtheirus salmonis 14 June, 2018 - 12:37
394 AN F336 EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult 60 Lepeophtheirus salmonis F>75.00% 14 June, 2018 - 12:44
395 AN F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua PreAdult1, Adult Control 60 Lepeophtheirus salmonis 14 June, 2018 - 12:48
396 EMLSAT00000002095_copepodites EMLSAT00000002095, EMLSAT00000002095-697942 (mRNA) Lepeophtheirus salmonis
Astacin
Copepodid, Copepodid host related phenotype, modulation of immune response 420/50 Lepeophtheirus salmonis F>94.00% 14 June, 2018 - 13:12
397 EMLSAT00000009839_Var1Frag1 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Nauplius1, Copepodid no visible phenotype Lepeophtheirus salmonis F>59.00% 14 June, 2018 - 13:28
398 RNAi_AN 10832+11366+11369+12062 EMLSAG00000010832, EMLSAG00000010832-693598 (gene) Lepeophtheirus salmonis, EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis preadult I male, preadult I female developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 14 June, 2018 - 13:31
399 Nauplii I 7974-1 EMLSAG00000007974, EMLSAG00000007974-690740 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:34
400 EMLSAT00000009839_Var1Frag2 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Nauplius1, Copepodid no visible phenotype Lepeophtheirus salmonis F>66.00% 14 June, 2018 - 13:34

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