RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
51 E F42 EMLSAT00000005071, EMLSAT00000005071-700918 (mRNA) Lepeophtheirus salmonis
g13677_Rheb
PreAdult2, Adult Low reproduction and most are missing blood in the
feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings
39/25 Lepeophtheirus salmonis F>60.00% 12 March, 2013 - 10:38
Molecular characterization and functional analysis of...
52 Z F214 EMLSAG00000000426, EMLSAG00000000426-683192 (gene) Lepeophtheirus salmonis
426
PreAdult2, Adult Many bad and short eggstrings.
female reproduction, shorter eggstrings
30/19 Lepeophtheirus salmonis 3 September, 2015 - 09:21
53 E F50 (Ferr_sub) EMLSAG00000010792, EMLSAG00000010792-693558 (gene) Lepeophtheirus salmonis
ferr_sub
PreAdult2, Adult Most lice didn’t have egg strings at termination (
female reproduction, no eggstrings
38/18 Lepeophtheirus salmonis F>85.00% 21 February, 2013 - 11:54
54 E F51 (Ferr_LH) EMLSAT00000006305, EMLSAT00000006305-702152 (mRNA) Lepeophtheirus salmonis
ferr_LH
PreAdult2, Adult Most lice didn’t have egg strings at termination (
female reproduction, no eggstrings
39/23 Lepeophtheirus salmonis F>78.00% 28 June, 2013 - 11:43
55 HCP1 EMLSAT00000003292, EMLSAT00000003292-699139 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No difference in heme levels
no visible phenotype
30 Lepeophtheirus salmonis F>80.00% 23 April, 2018 - 10:59
56 AP F366 EMLSAT00000001458 EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
EMLSAG00000001458
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>77.00% 29 May, 2018 - 11:26
57 AQ F375 EMLSAG00000008959 EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis
EMLSAG00000008959
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/5 Lepeophtheirus salmonis 29 May, 2018 - 14:58
58 D F39 EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis
EcR
PreAdult2 No eggstring production
female reproduction, no eggstrings
39/16 Lepeophtheirus salmonis F>0.00% 12 December, 2014 - 10:54
Molecular characterisation of the salmon louse,...
59 AF F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
PreAdult2, Adult no eggstrings
Control
30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:40
60 B F18 EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis
EcR
PreAdult2 No eggstrings 39/14 Lepeophtheirus salmonis F>0.00% 12 December, 2014 - 10:39
Molecular characterisation of the salmon louse,...

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