RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
61 AQ F375 EMLSAG00000008959 EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis
EMLSAG00000008959
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/5 Lepeophtheirus salmonis 29 May, 2018 - 14:58
62 D F39 EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis
EcR
PreAdult2 No eggstring production
female reproduction, no eggstrings
39/16 Lepeophtheirus salmonis F>0.00% 12 December, 2014 - 10:54
Molecular characterisation of the salmon louse,...
63 RNAi_U 5667 EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis PreAdult2 no eggstrings 120/28 Lepeophtheirus salmonis F>50.00% 10 June, 2015 - 10:15
64 AF F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
PreAdult2, Adult no eggstrings
Control
30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:40
65 B F18 EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis
EcR
PreAdult2 No eggstrings 39/14 Lepeophtheirus salmonis F>0.00% 12 December, 2014 - 10:39
Molecular characterisation of the salmon louse,...
66 AL F338 EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis, EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
FTZ-F1
PreAdult2, Adult No eggstrings
female reproduction, no eggstrings
30/12 Lepeophtheirus salmonis F>45.00% 14 June, 2018 - 12:56
67 AL F339 EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis, EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
FTZ-F1
PreAdult2, Adult No eggstrings
female reproduction, no eggstrings
30/7 Lepeophtheirus salmonis F>45.00% 14 June, 2018 - 13:14
68 AL Hormone receptor 3 (HR3) EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis
EMLSAG00000000733
PreAdult2 No eggstrings.
female reproduction, no eggstrings
30/17 Lepeophtheirus salmonis F>90.00% 14 June, 2018 - 10:47
69 RNAi_V 5667 female EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis PreAdult1 no eggstrings, no spermatophore 60/12 Lepeophtheirus salmonis 14 January, 2015 - 08:15
70 EMLSAT00000001458 cop + infection EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
RAB1A
Nauplius1, Copepodid no lice survived to the end of experiment, where c
survival, reduced
60 Lepeophtheirus salmonis 14 June, 2018 - 11:36

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