RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
101 AX F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal 30/22 Lepeophtheirus salmonis 5 August, 2019 - 10:18
102 AX F259 EMLSAG00000012823, EMLSAG00000012823-695589 (gene) Lepeophtheirus salmonis
T6_12823
PreAdult2 normal 30/13 Lepeophtheirus salmonis 5 August, 2019 - 13:45
103 AX F260 EMLSAT00000011145, EMLSAT00000011145-706992 (mRNA) Lepeophtheirus salmonis
T16_11145
PreAdult2 normal 30/21 Lepeophtheirus salmonis 5 August, 2019 - 13:42
104 AX F262 EMLSAG00000006928, EMLSAG00000006928-689694 (gene) Lepeophtheirus salmonis
T19_6928
PreAdult2 normal 30/14 Lepeophtheirus salmonis 5 August, 2019 - 13:39
105 AX F418 EMLSAT00000010925, EMLSAT00000010925-706772 (mRNA) Lepeophtheirus salmonis
Trypsin- Dylan
PreAdult2 survival, reduced 30/3 Lepeophtheirus salmonis 5 August, 2019 - 13:49
106 beta FTZ-F1 EMLSAT00000008900, EMLSAT00000008900-704747 (mRNA) Lepeophtheirus salmonis, EMLSAT00000008902, EMLSAT00000008902-704749 (mRNA) Lepeophtheirus salmonis
beta FTZ-F1
Nauplius, Copepodid Developmental arrest
developmental defect, Molting, no molting
0 Lepeophtheirus salmonis F>50.00% 14 June, 2018 - 13:50
107 B F18 EMLSAG00000005090, EMLSAG00000005090-687856 (gene) Lepeophtheirus salmonis
EcR
PreAdult2 No eggstrings 39/14 Lepeophtheirus salmonis F>0.00% 12 December, 2014 - 10:39
Molecular characterisation of the salmon louse,...
108 B F21 EMLSAT00000000705, EMLSAT00000000705-696552 (mRNA) Lepeophtheirus salmonis
LsCP1
PreAdult2 no visible phenotype 30/21 Lepeophtheirus salmonis F>80.00% 15 October, 2013 - 11:18
109 B F2+F3 (IRP1+IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1B, LsIRP1A
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>44.00% 29 April, 2015 - 16:21
Characterisation of iron regulatory protein 1A and 1B in the...
110 C F22 EMLSAT00000003030, EMLSAT00000003030-698877 (mRNA) Lepeophtheirus salmonis
g1751_Tryp1
PreAdult2 39/5 Lepeophtheirus salmonis F>94.00% 4 February, 2015 - 13:04

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