RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
111 C F23 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
g3359_Tryp2
PreAdult2 40/10 Lepeophtheirus salmonis F>0.10% 4 February, 2015 - 13:14
112 C F40 EMLSAG00000000441, EMLSAG00000000441-683207 (gene) Lepeophtheirus salmonis
PGES2
PreAdult2 no visible phenotype
no visible phenotype
30/9 Lepeophtheirus salmonis F>0.00% 15 October, 2013 - 12:53
Molecular characterization and knock-down of salmon louse (...
113 C F4 (CopB2) EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis
LsCopB2
PreAdult2, Adult feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings, survival, reduced 39 Lepeophtheirus salmonis F>82.00% 24 July, 2015 - 16:11
RNA interference mediated knockdown of the KDEL receptor and...
114 D F26 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
g10296_cath
PreAdult2 No visible phenotype 40/7 Lepeophtheirus salmonis F>80.00% 4 February, 2015 - 13:21
115 D F28 EMLSAT00000006302, EMLSAT00000006302-702149 (mRNA) Lepeophtheirus salmonis
g158_glycin_trans
PreAdult2 40/12 Lepeophtheirus salmonis F>42.00% 4 February, 2015 - 13:29
116 D F29 EMLSAG00000006214, EMLSAG00000006214-688980 (gene) Lepeophtheirus salmonis
g2282_trehalose
PreAdult2 a bit short eggstrings (?), other wise normal.
female reproduction
36/12 Lepeophtheirus salmonis F>59.00% 6 March, 2013 - 14:49
117 D F31 EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis
TOR
PreAdult2 female reproduction, shorter eggstrings 40/14 Lepeophtheirus salmonis F>0.10% 4 February, 2015 - 13:36
Molecular characterization and functional analysis of...
118 D F32 EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis
NLG3408_TSC1
PreAdult2 no visible phenotype 40/7 Lepeophtheirus salmonis F>33.00% 4 February, 2015 - 13:46
Molecular characterization and functional analysis of...
119 D F32/F33 EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis, EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis
g18363_TSC2, NLG3408_TSC1
PreAdult2 female reproduction, shorter eggstrings 40/14 Lepeophtheirus salmonis F>30.00% 5 February, 2015 - 08:46
Molecular characterization and functional analysis of...
120 D F33 EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis
g18363_TSC2
PreAdult2 female reproduction, shorter eggstrings 40/11 Lepeophtheirus salmonis F>72.00% 4 February, 2015 - 13:53
Molecular characterization and functional analysis of...

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