RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
41 S F183 EMLSAT00000011090, EMLSAT00000011090-706937 (mRNA) Lepeophtheirus salmonis PreAdult2 Normal egg strings 30/18 Lepeophtheirus salmonis 26 February, 2015 - 15:15
42 S F180 EMLSAT00000009473, EMLSAT00000009473-705320 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype but significant reduction in 30/24 Lepeophtheirus salmonis 26 February, 2015 - 14:41
43 S F179 EMLSAG00000011173, EMLSAG00000011173-693939 (gene) Lepeophtheirus salmonis PreAdult2 No visible phenotype 30/18 Lepeophtheirus salmonis 26 February, 2015 - 14:28
44 S F172 (FLVCR) EMLSAG00000007480, EMLSAG00000007480-690246 (gene) Lepeophtheirus salmonis
FLVCR
Adult, Adult no visible phenotype 29/24 Lepeophtheirus salmonis 27 July, 2015 - 13:27
45 S F164 EMLSAT00000009656, EMLSAT00000009656-705503 (mRNA) Lepeophtheirus salmonis
UN47_9656
Adult Normal
no visible phenotype
30/22 Lepeophtheirus salmonis F>99.00% 20 October, 2014 - 11:17
46 S F163 EMLSAT00000010954, EMLSAT00000010954-706801 (mRNA) Lepeophtheirus salmonis
UN46_10954
Adult Some abnormal
no visible phenotype
31/21 Lepeophtheirus salmonis F>5.00% 20 October, 2014 - 11:22
47 S F162 EMLSAT00000008265, EMLSAT00000008265-704112 (mRNA) Lepeophtheirus salmonis
UN41_3352
Adult Normal
no visible phenotype
30/21 Lepeophtheirus salmonis F>99.00% 20 October, 2014 - 11:11
48 S F0 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
CPY 185
Adult Control 30/20 Lepeophtheirus salmonis 17 November, 2014 - 11:31
49 RNAi_V 5667 male EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis PreAdult2 no spermatophore deposition 60/10 Lepeophtheirus salmonis 14 January, 2015 - 08:17
50 RNAi_V 5667 female EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis PreAdult1 no eggstrings, no spermatophore 60/12 Lepeophtheirus salmonis 14 January, 2015 - 08:15

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