RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
151 ExpH - EMLSAT000000010949 EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis
Trypsin
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>93.00% 14 June, 2018 - 10:26
152 F0 cop + infection CPY185 (negative control), CPY185 (PCR_product) Gadus morhua Nauplius1, Copepodid No visible phenotype 60/52 Lepeophtheirus salmonis 2 May, 2019 - 11:21
153 F149 EMLSAG00000010881, EMLSAG00000010881-693647 (gene) Lepeophtheirus salmonis Nauplius1, Copepodid Development of gut affected
developmental defect, organ development, intestine
200/190 Lepeophtheirus salmonis F>80.00% 11 June, 2014 - 14:07
154 F159 EMLSAG00000001069, EMLSAG00000001069-683835 (gene) Lepeophtheirus salmonis
Peroxidase
PreAdult1 female, PreAdult2 male Reduced survival 90/11 Lepeophtheirus salmonis F>90.00% 11 June, 2014 - 11:16
155 F159 EMLSAG00000001069, EMLSAG00000001069-683835 (gene) Lepeophtheirus salmonis Nauplius1, Copepodid Slow movement 200/190 Lepeophtheirus salmonis F>90.00% 11 June, 2014 - 11:28
156 F18 EMLSAT00000005090, EMLSAT00000005090-700937 (mRNA) Lepeophtheirus salmonis Nauplius 200/150 Lepeophtheirus salmonis F>50.00% 16 January, 2015 - 13:10
157 F18/F84 EMLSAT00000005090, EMLSAT00000005090-700937 (mRNA) Lepeophtheirus salmonis
EcR, USP
Nauplius No molting 200/150 Lepeophtheirus salmonis 16 January, 2015 - 13:20
The Ecdysone Receptor (EcR) is a Major Regulator of Tissue...
158 F18/F84 EMLSAT00000005090, EMLSAT00000005090-700937 (mRNA) Lepeophtheirus salmonis
EcR, UsP
Nauplius No molting 200/150 Lepeophtheirus salmonis F>50.00% 16 January, 2015 - 13:38
The Ecdysone Receptor (EcR) is a Major Regulator of Tissue...
159 F230 Nauplia EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Nauplius I, chalamus II/padI No phenotype
no visible phenotype
550/240 Lepeophtheirus salmonis F>98.50% 21 February, 2017 - 10:25
160 F231 Nauplia EMLSAT00000005273, EMLSAT00000005273-701120 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005273
Nauplius I, chalamus II/padI 550/201 Lepeophtheirus salmonis F>96.50% 17 March, 2017 - 14:25

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