RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
21 U F52 EMLSAG00000010179, EMLSAG00000010179-692945 (gene) Lepeophtheirus salmonis
g4675
Adult, Adult Non-hatching eggstrings
female reproduction, abnormal egg strings
30/10 Lepeophtheirus salmonis 8 December, 2014 - 13:56
22 U F187 EMLSAG00000011947, EMLSAG00000011947-694713 (gene) Lepeophtheirus salmonis PreAdult2, Adult Normal, hatching data yet to come
no visible phenotype
29/16 Lepeophtheirus salmonis F>95.00% 8 December, 2014 - 13:53
23 U F165 EMLSAG00000007327, EMLSAG00000007327-690093 (gene) Lepeophtheirus salmonis
UN49_7327
PreAdult2, Adult no visible phenotype 30/10 Lepeophtheirus salmonis F>96.00% 8 December, 2014 - 13:51
24 U F160 EMLSAG00000010462, EMLSAG00000010462-693228 (gene) Lepeophtheirus salmonis
UN36_10462
PreAdult2, Adult no visible phenotype 28/14 Lepeophtheirus salmonis F>99.00% 8 December, 2014 - 13:49
25 U F0 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
CPY185
PreAdult2, Adult Control 29/15 Lepeophtheirus salmonis 8 December, 2014 - 13:47
26 T F182 EMLSAG00000012145, EMLSAG00000012145-694911 (gene) Lepeophtheirus salmonis PreAdult2 some animals with Zig Zag egg strings + 40% reduct 30/12 Lepeophtheirus salmonis 26 February, 2015 - 15:39
27 T F181 EMLSAG00000001530, EMLSAG00000001530-684296 (gene) Lepeophtheirus salmonis PreAdult2 All animals with short and abnormal egg strings, f 30/10 Lepeophtheirus salmonis 26 February, 2015 - 15:51
28 T F178 EMLSAT00000003607, EMLSAT00000003607-699454 (mRNA) Lepeophtheirus salmonis
3607
PreAdult2, Adult A few slightly abnormal 28/14 Lepeophtheirus salmonis F>0.00% 14 November, 2014 - 13:20
29 T F177 EMLSAT00000000574, EMLSAT00000000574-696421 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No visible phenotype
no visible phenotype
30/19 Lepeophtheirus salmonis F>0.00% 14 November, 2014 - 13:47
30 T F169 EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis
UN53_5145, LsMvp1
PreAdult2, Adult normal
no visible phenotype
29/13 Lepeophtheirus salmonis F>67.00% 14 November, 2014 - 13:41

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