RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort ascending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
351 AP F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3
PreAdult2 normal
no visible phenotype
30/15 Lepeophtheirus salmonis F>68.00% 13 April, 2018 - 09:45
352 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 27 March, 2018 - 13:43
353 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:32
354 AP F352 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_1
PreAdult2, Adult no visible phenotype 30/20 Lepeophtheirus salmonis F>80.00% 27 March, 2018 - 13:36
355 AP F247 EMLSAG00000005706 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
31 Lepeophtheirus salmonis 29 May, 2018 - 13:13
356 AP F247 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
T11_5706
PreAdult2, Adult no visible phenotype 31/20 Lepeophtheirus salmonis 27 March, 2018 - 13:57
357 AP F246 EMLSAT00000008875, EMLSAT00000008875-704722 (mRNA) Lepeophtheirus salmonis
T9_8875
PreAdult2, Adult no visible phenotype 30/16 Lepeophtheirus salmonis 27 March, 2018 - 13:54
358 AP F245 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
T10_8281
PreAdult2, Adult no visible phenotype 31/24 Lepeophtheirus salmonis 27 March, 2018 - 14:00
359 AP F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/17 Lepeophtheirus salmonis 27 March, 2018 - 14:02
360 AO F359 EMLSAT00000010263, EMLSAT00000010263-706110 (mRNA) Lepeophtheirus salmonis
EMLSAT00000010263
PreAdult2 30 Lepeophtheirus salmonis F>60.00% 26 March, 2018 - 11:06

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