RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
381 RNAi_E 3971 EMLSAG00000003971, EMLSAG00000003971-686737 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 2 May, 2018 - 13:47
382 RNAi_I 3203 EMLSAG00000003203, EMLSAG00000003203-685969 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 2 May, 2018 - 13:49
383 RNAi_I 6629 EMLSAG00000006629, EMLSAG00000006629-689395 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 2 May, 2018 - 13:51
384 RNAi_I 8363+5310 EMLSAG00000008363, EMLSAG00000008363-691129 (gene) Lepeophtheirus salmonis, EMLSAG00000005310, EMLSAG00000005310-688076 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 2 May, 2018 - 13:54
385 RNAi_I 8363+6629+5310 EMLSAG00000008363, EMLSAG00000008363-691129 (gene) Lepeophtheirus salmonis, EMLSAG00000006629, EMLSAG00000006629-689395 (gene) Lepeophtheirus salmonis, EMLSAG00000005310, EMLSAG00000005310-688076 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 2 May, 2018 - 13:56
386 Nauplii I 12262+22100+4547+12296+12453+9341 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis, EMLSAG00000012453, EMLSAG00000012453-695219 (gene) Lepeophtheirus salmonis, EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis Nauplius1 Lepeophtheirus salmonis 2 May, 2018 - 14:36
387 F367 EMLSAT00000005299 nauplia EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAT00000005299
Nauplius1 200 Lepeophtheirus salmonis 29 May, 2018 - 15:16
388 AQ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 Control 30/5 Lepeophtheirus salmonis 6 June, 2018 - 09:56
389 ExpH - EMLSAT000000010949 EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis
Trypsin
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>93.00% 14 June, 2018 - 10:26
390 ExpH - EMLSAT000000006642 EMLSAT00000006642, EMLSAT00000006642-702489 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>98.00% 14 June, 2018 - 10:57

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