RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort descending
61 Q F0 db ctrl placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
CPY 185
PreAdult2, Adult Reduced survival 79 Lepeophtheirus salmonis 11 June, 2014 - 14:43
62 M F117 EMLSAG00000001500, EMLSAG00000001500-684266 (gene) Lepeophtheirus salmonis PreAdult2, Adult 40/12 Lepeophtheirus salmonis 13 June, 2014 - 13:14
63 R F161 EMLSAG00000000580, EMLSAG00000000580-683346 (gene) Lepeophtheirus salmonis
UN38_580
PreAdult2, Adult Abnormal eggstrings in some individuals
female reproduction, abnormal egg strings
37/20 Lepeophtheirus salmonis F>99.00% 7 October, 2014 - 10:17
64 R F124 EMLSAG00000012828, EMLSAG00000012828-695594 (gene) Lepeophtheirus salmonis
UN32_g22197
PreAdult2 Normal
no visible phenotype
39/24 Lepeophtheirus salmonis 7 October, 2014 - 10:26
65 S F162 EMLSAT00000008265, EMLSAT00000008265-704112 (mRNA) Lepeophtheirus salmonis
UN41_3352
Adult Normal
no visible phenotype
30/21 Lepeophtheirus salmonis F>99.00% 20 October, 2014 - 11:11
66 S F164 EMLSAT00000009656, EMLSAT00000009656-705503 (mRNA) Lepeophtheirus salmonis
UN47_9656
Adult Normal
no visible phenotype
30/22 Lepeophtheirus salmonis F>99.00% 20 October, 2014 - 11:17
67 S F163 EMLSAT00000010954, EMLSAT00000010954-706801 (mRNA) Lepeophtheirus salmonis
UN46_10954
Adult Some abnormal
no visible phenotype
31/21 Lepeophtheirus salmonis F>5.00% 20 October, 2014 - 11:22
68 R F158 EMLSAT00000009936, EMLSAT00000009936-705783 (mRNA) Lepeophtheirus salmonis PreAdult2 Normal
no visible phenotype
39/20 Lepeophtheirus salmonis F>95.00% 20 October, 2014 - 15:32
69 T F178 EMLSAT00000003607, EMLSAT00000003607-699454 (mRNA) Lepeophtheirus salmonis
3607
PreAdult2, Adult A few slightly abnormal 28/14 Lepeophtheirus salmonis F>0.00% 14 November, 2014 - 13:20
70 T F166 EMLSAT00000010071, EMLSAT00000010071-705918 (mRNA) Lepeophtheirus salmonis
UN50_10071
PreAdult2, Adult Abnormal
female reproduction, abnormal egg strings
30/19 Lepeophtheirus salmonis F>96.00% 14 November, 2014 - 13:26

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