RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
11 AV F350 EMLSAG00000009839, EMLSAG00000009839-692605 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype
30/20 Lepeophtheirus salmonis 2 May, 2019 - 11:17
12 AR F377 EMLSAT00000010626 EMLSAG00000010626, EMLSAG00000010626-693392 (gene) Lepeophtheirus salmonis
EMLSAG00000010626
PreAdult2 No visible phenotype 30/13 Lepeophtheirus salmonis 2 May, 2019 - 10:57
13 AR F249 EMLSAG00000010968, EMLSAG00000010968-693734 (gene) Lepeophtheirus salmonis PreAdult2 No visible phenotype 30/11 Lepeophtheirus salmonis 2 May, 2019 - 10:54
14 AR F244 EMLSAG00000010957, EMLSAG00000010957-693723 (gene) Lepeophtheirus salmonis PreAdult2 No visible phenotype 30/15 Lepeophtheirus salmonis 2 May, 2019 - 10:50
15 AV F0 extra CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal
no visible phenotype
30/17 Lepeophtheirus salmonis 2 May, 2019 - 09:59
16 AU F0 male CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
Adult No visible phenotype
Control
30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:56
17 Heme peroxidase F415 EMLSAT00000013035, EMLSAT00000013035-708882 (mRNA) Lepeophtheirus salmonis, EMLSAT00000013034, EMLSAT00000013034-708881 (mRNA) Lepeophtheirus salmonis
hpx
Adult 35 Lepeophtheirus salmonis 2 May, 2019 - 09:42
18 AV F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3
PreAdult2 normal
no visible phenotype
30/15 Lepeophtheirus salmonis 2 May, 2019 - 09:38
19 AU F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult No visible phenotype
Control
30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:37
20 AR F385 EMLSAT00000007048 EMLSAT00000007048, EMLSAT00000007048-702895 (mRNA) Lepeophtheirus salmonis
EMLSAG00000007048
PreAdult2 No visible phenotype
no visible phenotype
30/12 Lepeophtheirus salmonis 2 January, 2019 - 14:10

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