RNAi

Search or browse a total of 385 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
11 AV F352 EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype
30/11 Lepeophtheirus salmonis 2 May, 2019 - 11:24
12 F0 cop + infection CPY185 (negative control), CPY185 (PCR_product) Gadus morhua Nauplius1, Copepodid No visible phenotype 60/52 Lepeophtheirus salmonis 2 May, 2019 - 11:21
13 AV F350 EMLSAG00000009839, EMLSAG00000009839-692605 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype
30/20 Lepeophtheirus salmonis 2 May, 2019 - 11:17
14 AT F393 + F149 EMLSAT00000004598, EMLSAT00000004598-700445 (mRNA) Lepeophtheirus salmonis PreAdult2 No Visible Phenotype 40/21 Lepeophtheirus salmonis F>70.00% 2 May, 2019 - 11:01
15 AR F377 EMLSAT00000010626 EMLSAG00000010626, EMLSAG00000010626-693392 (gene) Lepeophtheirus salmonis
EMLSAG00000010626
PreAdult2 No visible phenotype 30/13 Lepeophtheirus salmonis 2 May, 2019 - 10:57
16 AR F249 EMLSAG00000010968, EMLSAG00000010968-693734 (gene) Lepeophtheirus salmonis PreAdult2 No visible phenotype 30/11 Lepeophtheirus salmonis 2 May, 2019 - 10:54
17 AR F244 EMLSAG00000010957, EMLSAG00000010957-693723 (gene) Lepeophtheirus salmonis PreAdult2 No visible phenotype 30/15 Lepeophtheirus salmonis 2 May, 2019 - 10:50
18 AU F336 EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis Adult no visible phenotype 30/6 Lepeophtheirus salmonis 2 May, 2019 - 10:16
19 AV F0 extra CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal
no visible phenotype
30/17 Lepeophtheirus salmonis 2 May, 2019 - 09:59
20 AU F0 male CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
Adult No visible phenotype
Control
30/8 Lepeophtheirus salmonis 2 May, 2019 - 09:56

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