RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
11 4485_1 Naupli EMLSAG00000004485, EMLSAG00000004485-687251 (gene) Lepeophtheirus salmonis
Ultrabithorax frag1 		Nauplius, Copepodid No visible phenotype
no visible phenotype 		Lepeophtheirus salmonis 27 March, 2018 - 10:18
12 4485_2 Naupli EMLSAG00000004485, EMLSAG00000004485-687251 (gene) Lepeophtheirus salmonis
Ultrabithorax frag2 		Nauplius, Copepodid No visible phenotype
no visible phenotype 		Lepeophtheirus salmonis 27 March, 2018 - 10:29
13 5061 EMLSAT00000005061, EMLSAT00000005061-700908 (mRNA) Lepeophtheirus salmonis
Opsin 		Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 16:00
14 5535 EMLSAT00000005535, EMLSAT00000005535-701382 (mRNA) Lepeophtheirus salmonis
heat shock factor 1 		Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 15:51
15 6502_1 Naupli EMLSAG00000006502, EMLSAG00000006502-689268 (gene) Lepeophtheirus salmonis
Proboscipedia frag1 		Nauplius, Copepodid No visible phenotype
no visible phenotype 		Lepeophtheirus salmonis 27 March, 2018 - 10:14
16 6502_2 Naupli EMLSAG00000006502, EMLSAG00000006502-689268 (gene) Lepeophtheirus salmonis
Proboscipedia frag2 		Nauplius, Copepodid No visible phenotype
no visible phenotype 		Lepeophtheirus salmonis 27 March, 2018 - 10:26
17 AA F222 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis PreAdult1 female, PreAdult2 male female reproduction, no spermatophore 120 Lepeophtheirus salmonis 20 October, 2015 - 14:33
18 AC F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		Adult Control 30/28 Lepeophtheirus salmonis 22 March, 2016 - 09:10
19 AC F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version 		Adult female reproduction, no eggstrings 31/21 Lepeophtheirus salmonis 31 March, 2016 - 09:56
20 AC F230 EMLSAG00000000504, EMLSAG00000000504-683270 (gene) Lepeophtheirus salmonis
504 		Adult 31/24 Lepeophtheirus salmonis 31 March, 2016 - 14:29

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