RNAi

Search or browse a total of 379 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending Publication
121 RNAi_AH 9128 EMLSAT00000009128, EMLSAT00000009128-704975 (mRNA) Lepeophtheirus salmonis PreAdult2 30 Lepeophtheirus salmonis 2 May, 2018 - 09:39
122 HCP1 EMLSAT00000003292, EMLSAT00000003292-699139 (mRNA) Lepeophtheirus salmonis PreAdult2, Adult No difference in heme levels
no visible phenotype
30 Lepeophtheirus salmonis F>80.00% 23 April, 2018 - 10:59
123 HCP1 EMLSAT00000003292, EMLSAT00000003292-699139 (mRNA) Lepeophtheirus salmonis Nauplius I, Copepodid none 300 Lepeophtheirus salmonis F>80.00% 23 April, 2018 - 10:31
124 LsHSCARB on free living stages EMLSAT00000005382, EMLSAT00000005382-701229 (mRNA) Lepeophtheirus salmonis, EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis Nauplius I, Copepodid none
no visible phenotype
300/250 Lepeophtheirus salmonis F>99.00% 23 April, 2018 - 10:06
A scavenger receptor B (CD36)-like protein is a potential...
125 Rhabdovirus - No9+127 prod of virus free strain LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9, LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127
N protein LsRV-No9, N protein LsRV-No127
Nauplius I, Adult no visible phenotype 480 Lepeophtheirus salmonis F>98.00% 20 April, 2018 - 10:02
RNAi-mediated treatment of two vertically transmitted...
126 Rhabdovirus - No9+127 prod of virus free strain LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9, LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127
N protein LsRV-No9, N protein LsRV-No127
Nauplius I, Adult no visible phenotype 480 Lepeophtheirus salmonis F>100.00% 20 April, 2018 - 10:01
RNAi-mediated treatment of two vertically transmitted...
127 Rhabdovirus - No9 prod of virus free strains LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9
N protein LsRV-No9
Nauplius I, Adult 480 Lepeophtheirus salmonis F>97.00% 20 April, 2018 - 09:38
RNAi-mediated treatment of two vertically transmitted...
128 Rhabdovirus - No9 prod of virus free strains LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9
N protein LsRV-No9
Nauplius I, Adult 480 Lepeophtheirus salmonis F>41.00% 20 April, 2018 - 09:35
RNAi-mediated treatment of two vertically transmitted...
129 Rhabdovirus - No9+127 Specificity LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9, LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127
N protein LsRV-No9, N protein LsRV-No127
Nauplius I, Copepodid 540/540 Lepeophtheirus salmonis F>94.00% 19 April, 2018 - 15:00
RNAi-mediated treatment of two vertically transmitted...
130 AP F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3
PreAdult2 normal
no visible phenotype
30/15 Lepeophtheirus salmonis F>68.00% 13 April, 2018 - 09:45

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