RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
121 10095mal EMLSAT00000010095, EMLSAT00000010095-705942 (mRNA) Lepeophtheirus salmonis
Prohibitin
Nauplius No visible phenotype Lepeophtheirus salmonis 28 March, 2018 - 10:04
122 2839/5061/10390 EMLSAT00000010390, EMLSAT00000010390-706237 (mRNA) Lepeophtheirus salmonis, EMLSAT00000002839, EMLSAT00000002839-698686 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005061, EMLSAT00000005061-700908 (mRNA) Lepeophtheirus salmonis
Opsins
Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 16:06
123 10390 EMLSAT00000010390, EMLSAT00000010390-706237 (mRNA) Lepeophtheirus salmonis
Opsin
Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 16:02
124 5061 EMLSAT00000005061, EMLSAT00000005061-700908 (mRNA) Lepeophtheirus salmonis
Opsin
Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 16:00
125 2839 EMLSAT00000002839, EMLSAT00000002839-698686 (mRNA) Lepeophtheirus salmonis
Opsin
Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 15:58
126 2965 EMLSAT00000002965, EMLSAT00000002965-698812 (mRNA) Lepeophtheirus salmonis
HSP70/HSC70
Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 15:54
127 5535 EMLSAT00000005535, EMLSAT00000005535-701382 (mRNA) Lepeophtheirus salmonis
heat shock factor 1
Nauplius No visible phenotype Lepeophtheirus salmonis 27 March, 2018 - 15:51
128 AP F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/17 Lepeophtheirus salmonis 27 March, 2018 - 14:02
129 AP F245 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
T10_8281
PreAdult2, Adult no visible phenotype 31/24 Lepeophtheirus salmonis 27 March, 2018 - 14:00
130 AP F247 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
T11_5706
PreAdult2, Adult no visible phenotype 31/20 Lepeophtheirus salmonis 27 March, 2018 - 13:57

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