RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort descending Publication
131 T F181 EMLSAG00000001530, EMLSAG00000001530-684296 (gene) Lepeophtheirus salmonis PreAdult2 All animals with short and abnormal egg strings, f 30/10 Lepeophtheirus salmonis 26 February, 2015 - 15:51
132 X F199 EMLSAG00000000147, EMLSAG00000000147-682913 (gene) Lepeophtheirus salmonis PreAdult2, Adult No visible phenotype
no visible phenotype
30/7 Lepeophtheirus salmonis 27 April, 2015 - 10:03
133 Y F0 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
CPY185
PreAdult2, Adult Control 30/11 Lepeophtheirus salmonis 27 April, 2015 - 10:07
134 X F196 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
E.coli
PreAdult2 30/11 Lepeophtheirus salmonis 27 April, 2015 - 14:31
135 A F2 (IRP1) EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1A
PreAdult2, Adult 39/25 Lepeophtheirus salmonis F>54.00% 28 April, 2015 - 17:24
Characterisation of iron regulatory protein 1A and 1B in the...
136 A F3 (IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis
LsIRP1B
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>45.00% 29 April, 2015 - 15:16
Characterisation of iron regulatory protein 1A and 1B in the...
137 A F4 (CopB2) EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis
LsCopB2
PreAdult2, Adult feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings, survival, reduced 39/5 Lepeophtheirus salmonis F>65.00% 29 April, 2015 - 15:44
RNA interference mediated knockdown of the KDEL receptor and...
138 B F2+F3 (IRP1+IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1B, LsIRP1A
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>44.00% 29 April, 2015 - 16:21
Characterisation of iron regulatory protein 1A and 1B in the...
139 RNAi_U 5667 EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis PreAdult2 no eggstrings 120/28 Lepeophtheirus salmonis F>50.00% 10 June, 2015 - 10:15
140 Y F200 EMLSAG00000000562, EMLSAG00000000562-683328 (gene) Lepeophtheirus salmonis
562
PreAdult2, Adult no visible phenotype 30/15 Lepeophtheirus salmonis F>21.00% 10 June, 2015 - 12:29

Pages