RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
21 AC F230 EMLSAT00000000504, EMLSAT00000000504-696351 (mRNA) Lepeophtheirus salmonis Adult, Adult No phenotype 30/24 Lepeophtheirus salmonis F>99.91% 12 April, 2016 - 10:35
22 AC F231 EMLSAT00000005273, EMLSAT00000005273-701120 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005273
Adult, Adult 30/25 Lepeophtheirus salmonis F>99.50% 12 April, 2016 - 11:31
23 A F2 (IRP1) EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1A
PreAdult2, Adult 39/25 Lepeophtheirus salmonis F>54.00% 28 April, 2015 - 17:24
Characterisation of iron regulatory protein 1A and 1B in the...
24 A F3 (IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis
LsIRP1B
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>45.00% 29 April, 2015 - 15:16
Characterisation of iron regulatory protein 1A and 1B in the...
25 A F4 (CopB2) EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis
LsCopB2
PreAdult2, Adult feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings, survival, reduced 39/5 Lepeophtheirus salmonis F>65.00% 29 April, 2015 - 15:44
RNA interference mediated knockdown of the KDEL receptor and...
26 AF F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2, Adult Control 30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:33
27 AF F0S CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY short version
PreAdult2, Adult no eggstrings
Control
30/16 Lepeophtheirus salmonis 16 July, 2016 - 11:40
28 AF F228 EMLSAG00000006953, EMLSAG00000006953-689719 (gene) Lepeophtheirus salmonis
6953
PreAdult2 30/15 Lepeophtheirus salmonis 16 July, 2016 - 11:45
29 AF F231 EMLSAT00000005273, EMLSAT00000005273-701120 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005273
padII/ad, Adult 30/14 Lepeophtheirus salmonis F>99.40% 17 March, 2017 - 12:50
30 AF F239 (F233+F234) EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis, EMLSAT00000002881, EMLSAT00000002881-698728 (mRNA) Lepeophtheirus salmonis
EMLSAG00000010949, EMLSAG00000002881
Adult, Adult 30/25 Lepeophtheirus salmonis 12 April, 2016 - 11:21

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