RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort descending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
401 Nauplii I 9433-2 EMLSAG00000009433, EMLSAG00000009433-692199 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:41
402 Nauplii I 4808-2 EMLSAG00000004808, EMLSAG00000004808-687574 (gene) Lepeophtheirus salmonis Nauplius I no visible phenotype Lepeophtheirus salmonis F>80.00% 14 June, 2018 - 13:43
403 Dead copepodid EMLSAT00000009839_Var1Frag1and2 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Nauplius1, Copepodid survival, reduced Lepeophtheirus salmonis 14 June, 2018 - 13:48
404 ExpD EMLSAT00000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Adult, Adult no visible phenotype 50/50 Lepeophtheirus salmonis F>99.00% 14 June, 2018 - 14:17
405 10397&5417 KD, first eggstring EMLSAT00000010397, EMLSAT00000010397-706244 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005417, EMLSAT00000005417-701264 (mRNA) Lepeophtheirus salmonis
EMLSAT00000010397, EMLSAT00000005417
PreAdult2 10 Lepeophtheirus salmonis 16 August, 2018 - 13:17
406 AS F379 EMLSAT00000008518, EMLSAT00000008518-704365 (mRNA) Lepeophtheirus salmonis
EMLSAT00000008518
PreAdult2 reproduction, female reproduction, abnormal egg strings 30/15 Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 09:46
407 RNAi_AS 11366+12062 EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 09:58
408 AS F380 EMLSAT00000000454, EMLSAT00000000454-696301 (mRNA) Lepeophtheirus salmonis
EMLSAT00000000454
PreAdult2 reproduction, female reproduction, no eggstrings 30/20 Lepeophtheirus salmonis F>99.00% 21 November, 2018 - 09:59
409 RNAi_AS 10832+11369 EMLSAG00000010832, EMLSAG00000010832-693598 (gene) Lepeophtheirus salmonis, EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:04
410 RNAi_AS 11369+12062 EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis, EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis
Nervana
preadult II femal developmental defect, feeding, female reproduction Lepeophtheirus salmonis F>90.00% 21 November, 2018 - 10:09

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