RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotypesort ascending Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted Publication
51 E F49a EMLSAT00000002641, EMLSAT00000002641-698488 (mRNA) Lepeophtheirus salmonis
ILR frag2
PreAdult2 35/22 Lepeophtheirus salmonis F>0.10% 4 February, 2015 - 14:57
52 E F49b EMLSAT00000002641, EMLSAT00000002641-698488 (mRNA) Lepeophtheirus salmonis
ILR frag2 halv dose
PreAdult2 40/25 Lepeophtheirus salmonis F>0.10% 4 February, 2015 - 14:59
53 H F24 EMLSAT00000002836, EMLSAT00000002836-698683 (mRNA) Lepeophtheirus salmonis
g3536_Tryp3
PreAdult2 40 Lepeophtheirus salmonis F>89.00% 4 February, 2015 - 15:12
54 D F32/F33 EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis, EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis
g18363_TSC2, NLG3408_TSC1
PreAdult2 female reproduction, shorter eggstrings 40/14 Lepeophtheirus salmonis F>30.00% 5 February, 2015 - 08:46
Molecular characterization and functional analysis of...
55 Y F0 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
CPY185
PreAdult2, Adult Control 30/11 Lepeophtheirus salmonis 27 April, 2015 - 10:07
56 X F196 placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
E.coli
PreAdult2 30/11 Lepeophtheirus salmonis 27 April, 2015 - 14:31
57 A F2 (IRP1) EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1A
PreAdult2, Adult 39/25 Lepeophtheirus salmonis F>54.00% 28 April, 2015 - 17:24
Characterisation of iron regulatory protein 1A and 1B in the...
58 A F3 (IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis
LsIRP1B
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>45.00% 29 April, 2015 - 15:16
Characterisation of iron regulatory protein 1A and 1B in the...
59 A F4 (CopB2) EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis
LsCopB2
PreAdult2, Adult feeding, reduced nutrient uptake, no blood uptake, female reproduction, no eggstrings, survival, reduced 39/5 Lepeophtheirus salmonis F>65.00% 29 April, 2015 - 15:44
RNA interference mediated knockdown of the KDEL receptor and...
60 B F2+F3 (IRP1+IRP2) EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis
LsIRP1B, LsIRP1A
PreAdult2, Adult female reproduction, shorter eggstrings 39/23 Lepeophtheirus salmonis F>44.00% 29 April, 2015 - 16:21
Characterisation of iron regulatory protein 1A and 1B in the...

Pages