RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
61 RNAi_E 3971+4146 EMLSAG00000003971, EMLSAG00000003971-686737 (gene) Lepeophtheirus salmonis, EMLSAG00000004146, EMLSAG00000004146-686912 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 14 June, 2018 - 13:10
62 EMLSAT00000003179 cop + infection EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis
Csdc2
Nauplius1, Copepodid no proper visible phenotype but maybe quicker deve
no visible phenotype
180/45 Lepeophtheirus salmonis 14 June, 2018 - 13:09
63 LsCHS1 EMLSAT00000002852, EMLSAT00000002852-698699 (mRNA) Lepeophtheirus salmonis
LsCHS1
PreAdult2 None 60/4 Lepeophtheirus salmonis 14 June, 2018 - 13:05
64 AL F338 EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis, EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
FTZ-F1
PreAdult2, Adult No eggstrings
female reproduction, no eggstrings
30/12 Lepeophtheirus salmonis F>45.00% 14 June, 2018 - 12:56
65 EMLSAT00000004874_Var2Frag2 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult no visible phenotype 60 Lepeophtheirus salmonis F>15.00% 14 June, 2018 - 12:53
66 EMLSAT00000004874_Var2Frag1 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult no visible phenotype 60 Lepeophtheirus salmonis F>20.00% 14 June, 2018 - 12:49
67 AN F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua PreAdult1, Adult Control 60 Lepeophtheirus salmonis 14 June, 2018 - 12:48
68 LsCHS2 EMLSAT00000007308, EMLSAT00000007308-703155 (mRNA) Lepeophtheirus salmonis
LsCHS2
PreAdult1 , Adult None 9 Lepeophtheirus salmonis F>60.00% 14 June, 2018 - 11:54
69 AN F337 EMLSAT00000010162, EMLSAT00000010162-706009 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult 60 Lepeophtheirus salmonis F>0.00% 14 June, 2018 - 11:51
70 EMLSAT00000009839_Var1Frag1 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult survival, reduced 60/5 Lepeophtheirus salmonis 14 June, 2018 - 11:48

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