RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Titlesort descending Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Posted
61 AP F247 EMLSAG00000005706 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
31 Lepeophtheirus salmonis 29 May, 2018 - 13:13
62 AP F352 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_1
PreAdult2, Adult no visible phenotype 30/20 Lepeophtheirus salmonis F>80.00% 27 March, 2018 - 13:36
63 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 27 March, 2018 - 13:43
64 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:32
65 AP F364 (F168+F169) EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis, EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
UN53_5145, UN52_5383, LsMvp1, LsMvp2/3
PreAdult2 normal
no visible phenotype
30/15 Lepeophtheirus salmonis F>68.00% 13 April, 2018 - 09:45
66 AP F365 EMLSAG00000003179 EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis PreAdult2 slightly shorter gonade and abdominal segment 30 Lepeophtheirus salmonis F>47.00% 29 May, 2018 - 12:46
67 AP F366 EMLSAT00000001458 EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
EMLSAG00000001458
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>77.00% 29 May, 2018 - 11:26
68 AP F367 EMLSAG00000005299 EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005299
PreAdult2 slightly shorter gonade and abdominal segment
reproduction, female reproduction, shorter eggstrings
30/24 Lepeophtheirus salmonis F>16.00% 29 May, 2018 - 12:27
69 AQ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 Control 30/5 Lepeophtheirus salmonis 6 June, 2018 - 09:56
70 AQ F372 EMLSAG00000011562 EMLSAT00000011562, EMLSAT00000011562-707409 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011562
PreAdult2 No visible phenotype
no visible phenotype
30/13 Lepeophtheirus salmonis 29 May, 2018 - 14:37

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