RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
71 EMLSAT00000001458 cop + infection EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
RAB1A
Nauplius1, Copepodid no lice survived to the end of experiment, where c
survival, reduced
60 Lepeophtheirus salmonis 14 June, 2018 - 11:36
72 ExpH - EMLSAT000000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>95.00% 14 June, 2018 - 11:08
73 AL Hormone receptor 3 (HR3) EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis
EMLSAG00000000733
PreAdult2 No eggstrings.
female reproduction, no eggstrings
30/17 Lepeophtheirus salmonis F>90.00% 14 June, 2018 - 10:47
74 AQ F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 Control 30/5 Lepeophtheirus salmonis 6 June, 2018 - 09:56
75 F367 EMLSAT00000005299 nauplia EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAT00000005299
Nauplius1 200 Lepeophtheirus salmonis 29 May, 2018 - 15:16
76 AQ F375 EMLSAG00000008959 EMLSAT00000008959, EMLSAT00000008959-704806 (mRNA) Lepeophtheirus salmonis
EMLSAG00000008959
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/5 Lepeophtheirus salmonis 29 May, 2018 - 14:58
77 AQ F372 EMLSAG00000011562 EMLSAT00000011562, EMLSAT00000011562-707409 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011562
PreAdult2 No visible phenotype
no visible phenotype
30/13 Lepeophtheirus salmonis 29 May, 2018 - 14:37
78 AQ F374 EMLSAG00000011382 EMLSAT00000011382, EMLSAT00000011382-707229 (mRNA) Lepeophtheirus salmonis
EMLSAG00000011382
PreAdult2 No visible phenotype
no visible phenotype
30/9 Lepeophtheirus salmonis 29 May, 2018 - 14:02
79 AQ F373 EMLSAG00000004269 EMLSAT00000004269, EMLSAT00000004269-700116 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
30/7 Lepeophtheirus salmonis 29 May, 2018 - 13:55
80 AP F247 EMLSAG00000005706 EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
31 Lepeophtheirus salmonis 29 May, 2018 - 13:13

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