RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
81 AP F365 EMLSAG00000003179 EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis PreAdult2 No visible phenotype
no visible phenotype
30 Lepeophtheirus salmonis F>47.00% 29 May, 2018 - 12:46
82 AP F367 EMLSAG00000005299 EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005299
PreAdult2 no visible phenotype
no visible phenotype
30/24 Lepeophtheirus salmonis F>16.00% 29 May, 2018 - 12:27
83 AP F366 EMLSAT00000001458 EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
EMLSAG00000001458
PreAdult2 no eggstreng
female reproduction, no eggstrings
30/13 Lepeophtheirus salmonis F>77.00% 29 May, 2018 - 11:26
84 LsCHS1 EMLSAT00000002852, EMLSAT00000002852-698699 (mRNA) Lepeophtheirus salmonis PreAdultI males In preAdultII: Could not complete molting or had t
Molting, developmental defect
60 Lepeophtheirus salmonis F>80.00% 7 May, 2018 - 23:13
85 Nauplii I 12262+22100+4547+12296+12453 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis, EMLSAG00000012453, EMLSAG00000012453-695219 (gene) Lepeophtheirus salmonis Nauplius I reproductive defect as adults
reproduction
Lepeophtheirus salmonis 2 May, 2018 - 14:43
86 Nauplii I 3422 EMLSAG00000003422, EMLSAG00000003422-686188 (gene) Lepeophtheirus salmonis Nauplius I Lepeophtheirus salmonis 2 May, 2018 - 14:42
87 Nauplii I 6012 EMLSAG00000006012, EMLSAG00000006012-688778 (gene) Lepeophtheirus salmonis Nauplius I Lepeophtheirus salmonis 2 May, 2018 - 14:40
88 Nauplii I 5667 EMLSAG00000005667, EMLSAG00000005667-688433 (gene) Lepeophtheirus salmonis Nauplius I Lepeophtheirus salmonis 2 May, 2018 - 14:38
89 Nauplii I 12262+22100+4547+12296+12453+9341 EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis, EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis, EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis, EMLSAG00000012453, EMLSAG00000012453-695219 (gene) Lepeophtheirus salmonis, EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis Nauplius1 Lepeophtheirus salmonis 2 May, 2018 - 14:36
90 RNAi_I 8363+6629+5310 EMLSAG00000008363, EMLSAG00000008363-691129 (gene) Lepeophtheirus salmonis, EMLSAG00000006629, EMLSAG00000006629-689395 (gene) Lepeophtheirus salmonis, EMLSAG00000005310, EMLSAG00000005310-688076 (gene) Lepeophtheirus salmonis PreAdult Lepeophtheirus salmonis 2 May, 2018 - 13:56

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