RNAi

Search or browse a total of 385 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
81 AN F336 EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis, EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis, EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult 60 Lepeophtheirus salmonis F>75.00% 14 June, 2018 - 12:44
82 EMLSAT00000009839_Var1Frag2 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult survival, reduced 60/3 Lepeophtheirus salmonis 14 June, 2018 - 12:37
83 LsCHS2 EMLSAT00000007308, EMLSAT00000007308-703155 (mRNA) Lepeophtheirus salmonis
LsCHS2
PreAdult1 , Adult None 9 Lepeophtheirus salmonis F>60.00% 14 June, 2018 - 11:54
84 AN F337 EMLSAT00000010162, EMLSAT00000010162-706009 (mRNA) Lepeophtheirus salmonis PreAdult1, Adult 60 Lepeophtheirus salmonis F>0.00% 14 June, 2018 - 11:51
85 EMLSAT00000009839_Var1Frag1 EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis
Heme peroxidase
Copepodid, PreAdult survival, reduced 60/5 Lepeophtheirus salmonis 14 June, 2018 - 11:48
86 EMLSAT00000001458 cop + infection EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
RAB1A
Nauplius1, Copepodid no lice survived to the end of experiment, where c
survival, reduced
60 Lepeophtheirus salmonis 14 June, 2018 - 11:36
87 ExpH - EMLSAT000000009293 EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>95.00% 14 June, 2018 - 11:08
88 ExpH - EMLSAT000000006642 EMLSAT00000006642, EMLSAT00000006642-702489 (mRNA) Lepeophtheirus salmonis
Unknown
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>98.00% 14 June, 2018 - 10:57
89 AL Hormone receptor 3 (HR3) EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis
EMLSAG00000000733
PreAdult2 No eggstrings.
female reproduction, no eggstrings
30/17 Lepeophtheirus salmonis F>90.00% 14 June, 2018 - 10:47
90 ExpH - EMLSAT000000010949 EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis
Trypsin
Copepodid, Copepodid no visible phenotype 640 Lepeophtheirus salmonis F>93.00% 14 June, 2018 - 10:26

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