BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:SSPO "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0007155 "cell adhesion" evidence=IEA] InterPro:IPR000421 InterPro:IPR000742 InterPro:IPR001007 InterPro:IPR002172 Pfam:PF00057 Pfam:PF00093 Pfam:PF00754 PRINTS:PR00261 PROSITE:PS01208 PROSITE:PS50022 PROSITE:PS50068 PROSITE:PS50184 SMART:SM00181 SMART:SM00192 SMART:SM00214 SMART:SM00231 GO:GO:0007155 InterPro:IPR002919 Pfam:PF01826 SUPFAM:SSF57567 Gene3D:2.60.120.260 InterPro:IPR008979 SUPFAM:SSF49785 Gene3D:4.10.400.10 InterPro:IPR023415 SUPFAM:SSF57424 PROSITE:PS01209 InterPro:IPR001846 Pfam:PF00094 SMART:SM00216 PROSITE:PS51233 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR014853 Pfam:PF08742 SMART:SM00832 InterPro:IPR006207 PROSITE:PS01225 InterPro:IPR006552 SMART:SM00215 InterPro:IPR028504 PANTHER:PTHR22905 GeneTree:ENSGT00730000110607 EMBL:AAEX03010265 Ensembl:ENSCAFT00000044018 Uniprot:J9P3R7)

HSP 1 Score: 89.3521 bits (220), Expect = 2.811e-16
Identity = 79/300 (26.33%), Postives = 116/300 (38.67%), Query Frame = 0

Query:  225 PSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN-IRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQ----------DCEWSDYDTWSVCSKPCGVGVQYRNRSIVH-PSRNGGNECPGRAFQSRICN 512
            P     SD+ G VDC  + +  WS CS +CG G   ++R +L+PS  GG SC  D + S+ C   A CPV   W+ +G W  CS +CGGG ++ +RN +       G PC G   E   CS  PC+               D D  +     +            P S        + +                       +  P    V   LK   +P       N SR   ++             C WS + +W  C + CG GV+ R RS  + P+ +GG  C G + + + C+
Sbjct: 2437 PDCQDGSDEDGCVDCRLAPWSAWSSCSRTCGPGLASQRRDVLRPSLPGG-SCPSDRLRSQPCFVQA-CPVAGAWAVWGPWGPCSVSCGGGHRSRQRNCVDPPPKNGGAPCPGASQEGAPCSLQPCT--------------GDTDCGLGRVHISAELCQKGLVPPCPPSCMDPEANRSCSGHCLEGCRCPPGLLLHDARCLPLSECPC--LVGEELKQPGVP---FLLDNCSRCLCEKGALLCEPGDCPVPCGWSAWSSWGPCDRSCGSGVRARFRSPSNPPAASGGAPCEGNSQELQACH 2715          

HSP 2 Score: 71.633 bits (174), Expect = 6.574e-11
Identity = 73/288 (25.35%), Postives = 107/288 (37.15%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSC---GTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLF--PSSTTTTTITTTTTTTSTTTTTPTST------TTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRS-IVHPSRNGGNECPGRAFQSRICNRHTC 516
            W+ +  WS CS SC   G G   R R  L PS  G  SC G+A +   C+    CPV   W  +  WS+CSA C GG Q     IR R+      C G  ++TR C+T PC+          S+     D         +  +  +  T F  P  T    +     +    +  P                 +    T +  E+    +  P+                    C WS +  WS+CS+ C VG++ R R+    P+  GG  C G   ++  C+   C
Sbjct: 2725 WTPWAPWSSCSRSCLTLGGGPGWRSRFRLCPSP-GDTSCPGEATQEEPCSP-PMCPVPSTWGLWAPWSTCSAPCDGGIQ-----IRGRSCSGDPECQGPHSQTRDCNTQPCTAQCPGDMVFRSAEQCQQDGGPCLQL-CLAQAPGVECTSFCAPGCTCPPGLFLHNASCLPRSRCPCQLHGRLYPLGAVALLDSCNNCTCVSGEMVCTSEPCPV-------------------ACGWSPWTPWSLCSRSCDVGIRRRFRAGTAPPASFGGAACQGPNMEAEFCSLRPC 2985          

HSP 3 Score: 60.077 bits (144), Expect = 1.741e-7
Identity = 75/291 (25.77%), Postives = 111/291 (38.14%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQG-GSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSS---ASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIES------RQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTN 346
            W  +  WS CS+SC GG++ R R       QG G  C G + +   C    CP         NC    + GA Y+ C           P + +  V+         +   G +  + G   +    SH     L + E    + + G R+    G      S    + +D    V   W  + +W+ CS  C + T+ R R+   P+  +GG  C G A E+        C +   CPV+  WS +G WS C   C G     R  +       GRPC G   ++R C  N
Sbjct: 3202 WGPWGPWSSCSRSCGGGLRSRMRACDQPPPQGLGDFCEGPQAQGEACQALPCP-------VTNC--TAIQGAEYSPC-------GPACPRSCDDLVHCEWHCQPGCYCPPGQVLSADGA--FCVQRSHCGCLDLLSGE----RHQPGARLPRPDGCNYCTCSEGRLNCTDLPCPVPGGWCPWSEWTACSQPCRSQTRTRSRVCTCPTPQHGGTPCPGKAGEAGAQHQKEACPSSPDCPVDGAWSPWGLWSPCD-PCLGQSHRSRACVWPPTPEGGRPCPGSYRQSRPCQDN 3469          

HSP 4 Score: 51.9878 bits (123), Expect = 5.526e-5
Identity = 65/241 (26.97%), Postives = 82/241 (34.02%), Query Frame = 0

Query:   48 LRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKIL-ILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCN 287
            L E+ A+     D  + C WS +SSW  C +SC  GV+ R R      A  GG+PC G  +E + C              V C    V+G T               PWA        +  +R      GG GW    R     G        DT  P E   EE                   S     V   W  +  WS CS  C  G Q R R     S +G   C+G   ++R CN
Sbjct: 2649 LCEKGALLCEPGDCPVPCGWSAWSSWGPCDRSCGSGVRARFRSPSNPPAASGGAPCEGNSQELQAC-------------HVEC-GTEVLGWT---------------PWA------PWSSCSRSCLTLGGGPGWRSRFRLCPSPG--------DTSCPGEATQEE-----------------PCSPPMCPVPSTWGLWAPWSTCSAPCDGGIQIRGR-----SCSGDPECQGPHSQTRDCN 2824          

HSP 5 Score: 51.6026 bits (122), Expect = 7.867e-5
Identity = 41/114 (35.96%), Postives = 54/114 (47.37%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRM-ILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCS 349
            V C WS +  WS CS SC  G ++R R     P++ GG +C+G  +E+  C+      +  EW   G WS CS  CG G        R R  GSG P L    +  SC   PC+
Sbjct: 2930 VACGWSPWTPWSLCSRSCDVGIRRRFRAGTAPPASFGGAACQGPNMEAEFCSLRPCRGLGGEW---GPWSPCSVPCGSG-------YRNRTRGSGLPGL---LDFSSCGLQPCA 3030          

HSP 6 Score: 50.447 bits (119), Expect = 1.705e-4
Identity = 67/291 (23.02%), Postives = 107/291 (36.77%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSS-PLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDD-DLSTTNNSRSSIKR-----VQDCEWSDYDTWSVCSKPCGV-GVQYRNRSIVHPSRNG-GNECPGRAFQSRICNRHTCGSGADQ 522
            W  +  W  CS +CG G Q R R    P     + C G   +++ C   A CPV+ +W+++  WS C+  C  G  T +R  +   +G            R+C+  P     A  + R    +   + + S      P +  PL        +     +     +S     P      S          P   +       Q I  +  L T  + R    R       +C WS +  W+ C  PCG   +Q+  RS  +P  +G G +C G   ++R C    C +G +Q
Sbjct: 1674 WGPWDSWGPCSQTCGPGVQGRSRHCSPPHLPVLQRCGGPEHQTQACFT-AACPVDGQWTSWSSWSLCTEPC-MGTMTRQRQCQHPQNGG-----------RTCAMLP-GGPHATRQIRPCPQDGCPNATCSGQLVFWPCAPCPLTCDEI---SGQAVCSANQPCSSPGCWCPVGQVLGSEGRCVWPRQCPCLLDGARYWPGQRIKANCQLCTCQDGRPQRCRPDPDCAVNCGWSSWSPWAECLGPCGSQNIQWSFRSPNNPRLSGQGRQCRGIHRKARRCQTEPC-AGCEQ 1946          

HSP 7 Score: 49.6766 bits (117), Expect = 2.407e-4
Identity = 39/123 (31.71%), Postives = 50/123 (40.65%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-------------GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNI-RRRASGSGRPCLGEKAETRSCSTNPC 348
            LWS +  WS CS SCG   + R R   +P T                  C G   E   C     C     W  +G WSSCS +CGGG ++  R   +    G G  C G +A+  +C   PC
Sbjct: 3132 LWSPWTPWSECSASCGPARRHRHRFCTRPPTTELTLKAPLSPPNLPTPLCPGPDAEEEPCLL-PGCDRAGGWGPWGPWSSCSRSCGGGLRSRMRACDQPPPQGLGDFCEGPQAQGEACQALPC 3253          

HSP 8 Score: 48.521 bits (114), Expect = 6.884e-4
Identity = 38/115 (33.04%), Postives = 52/115 (45.22%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQ-PSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATC--GGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            V C WS++  W  C  SCG+G + R R     P+ +GG  C G++ E + C+      V   W+ +  WSSCS +C   GG    R   R   S     C GE  +   CS   C
Sbjct: 2664 VPCGWSAWSSWGPCDRSCGSGVRARFRSPSNPPAASGGAPCEGNSQELQACHVECGTEVL-GWTPWAPWSSCSRSCLTLGGGPGWRSRFRLCPSPGDTSCPGEATQEEPCSPPMC 2777          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:bai1b "brain-specific angiogenesis inhibitor 1b" species:7955 "Danio rerio" [GO:0004930 "G-protein coupled receptor activity" evidence=IEA] [GO:0007186 "G-protein coupled receptor signaling pathway" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0016525 "negative regulation of angiogenesis" evidence=IEA] [GO:0007218 "neuropeptide signaling pathway" evidence=IEA] [GO:0004888 "transmembrane signaling receptor activity" evidence=IEA] [GO:0007166 "cell surface receptor signaling pathway" evidence=IEA] [GO:0005886 "plasma membrane" evidence=IEA] InterPro:IPR000203 InterPro:IPR000832 InterPro:IPR001879 InterPro:IPR008077 InterPro:IPR017981 Pfam:PF00002 Pfam:PF01825 PRINTS:PR00249 PRINTS:PR01694 PROSITE:PS50221 PROSITE:PS50227 PROSITE:PS50261 SMART:SM00303 ZFIN:ZDB-GENE-081031-45 GO:GO:0016021 GO:GO:0005886 GO:GO:0007218 GO:GO:0004930 GO:GO:0016525 EMBL:CU634003 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 TreeFam:TF331634 InterPro:IPR022624 Pfam:PF12003 GeneTree:ENSGT00690000101769 Ensembl:ENSDART00000109554 OMA:PEYSSHT ArrayExpress:F1QDN2 Bgee:F1QDN2 Uniprot:F1QDN2)

HSP 1 Score: 88.5817 bits (218), Expect = 3.452e-16
Identity = 80/289 (27.68%), Postives = 122/289 (42.21%), Query Frame = 0

Query:   60 DGRIDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            D  I   WS +  WS+CS  C GGVQ R R      +     C G  +E R C+ Q C   +G V   +  L ++IG      D    +  N+ P   E    + T  +  +   + G GW    R  + +                         +C+G  +++    +T+     V+  W  +  WS CS +CG G + R R   QP  NGG+ CRG   +++ CN  A CPV+  W+++  W+ CS++C  G     R     + G G  C GE  ET++C    C
Sbjct:  208 DAEIMKGWSGWGRWSECSSECGGGVQVRSRA----CQPEDGICEGVVEEGRACNPQPC---IGQVRQKSQGLRSIIGQRR---DSDGTLMANQAPQKEE--AQQDTWSSWSVCSVSCGEGWQSRTRSCMNSA------------------------QCIGPLRENRPCNNTA--VCPVNGAWDEWAPWSLCSSTCGRGYRDRVRTCKQPK-NGGEPCRGPFKQTKFCNI-AVCPVDGFWNDWSAWTPCSSSCSNGTTQRTRECNGPSYG-GSECRGEWLETKNCFLRDC 455          

HSP 2 Score: 72.7886 bits (177), Expect = 2.029e-11
Identity = 45/115 (39.13%), Postives = 62/115 (53.91%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCP---VNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            VD  W+ +  W+ CS SC  GT QR R    PS  GG  CRG+ +E++ C     CP   V+  W  +  W +CS +CGGG+Q  +R       G G+ C G+KAE R+C+   C
Sbjct:  402 VDGFWNDWSAWTPCSSSCSNGTTQRTRECNGPSY-GGSECRGEWLETKNCFL-RDCPGPLVDGRWLLWSSWGACSKSCGGGQQLRQRVCEGPFFG-GQSCNGDKAEMRNCNEKRC 513          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:SEMA5B "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0004872 "receptor activity" evidence=IEA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] InterPro:IPR001627 InterPro:IPR002165 InterPro:IPR015943 InterPro:IPR016201 Pfam:PF01403 Pfam:PF01437 PROSITE:PS51004 SMART:SM00423 SMART:SM00630 GO:GO:0007275 GO:GO:0016020 Gene3D:2.130.10.10 GO:GO:0004872 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 SUPFAM:SSF103575 SUPFAM:SSF101912 InterPro:IPR027231 PANTHER:PTHR11036 GeneTree:ENSGT00690000101974 OrthoDB:EOG7SN8C0 TreeFam:TF329951 OMA:SRSRHCE EMBL:CU633639 Ensembl:ENSSSCT00000012986 Uniprot:F1SQ38)

HSP 1 Score: 88.1965 bits (217), Expect = 3.701e-16
Identity = 91/285 (31.93%), Postives = 116/285 (40.70%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGL-DTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPC 348
            W+ + SWS CS +C GGVQ R R     A + G+ C G   E + C+ + CP    + P      VNV     A  + R++ T  + P                        G   G+R           SG  DTD       E+ +R            S  TS  T  V   W+++G WS CS  C  G + RKR    P   NGG  C GDA E + CN  A CPV   WS +  WS CSA+CGGG     R+    A   G   CLG   E   C+T  C
Sbjct:  572 WASWGSWSKCSSNCGGGVQSRSR-----ACENGNSCPGCGMEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGG-ARQEQRFRFTC-RAPLPDPH-------------------GLQFGRRRVETRTCPADGSGACDTD----ALVEDLLR------------SGGTSSHT--VSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQA-CPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQAC 811          

HSP 2 Score: 78.1814 bits (191), Expect = 4.414e-13
Identity = 43/109 (39.45%), Postives = 60/109 (55.05%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            W+ +  W+ CS SCG G Q R+R    P+  +GG+ C G + E R CN    CPV   W+++G WS CS+ CGGG Q+     R RA  +G  C G   E ++C+   C
Sbjct:  514 WTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQS-----RSRACENGNSCPGCGMEFKTCNPEGC 617          

HSP 3 Score: 48.9062 bits (115), Expect = 4.471e-4
Identity = 21/51 (41.18%), Postives = 34/51 (66.67%), Query Frame = 0

Query:  466 WSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHT 515
            W+ + +W++CS  CG+G Q R RS  +P+ R+GG  C G++ + R CN +T
Sbjct:  514 WTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENT 564          

HSP 4 Score: 48.1358 bits (113), Expect = 6.886e-4
Identity = 37/113 (32.74%), Postives = 50/113 (44.25%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA---SGSGRPCLGEKAETRSCSTN 346
            V   W+S+G WS+CS +CG G Q R R     +   G SC G  +E + CN    CP     + +  W   + T GG RQ  R     RA      G      + ETR+C  +
Sbjct:  568 VPIFWASWGSWSKCSSNCGGGVQSRSR-----ACENGNSCPGCGMEFKTCNPEG-CPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRVETRTCPAD 674          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:SEMA5A "Semaphorin-5A" species:9606 "Homo sapiens" [GO:0001569 "patterning of blood vessels" evidence=IEA] [GO:0001938 "positive regulation of endothelial cell proliferation" evidence=ISS] [GO:0002043 "blood vessel endothelial cell proliferation involved in sprouting angiogenesis" evidence=ISS] [GO:0005886 "plasma membrane" evidence=TAS] [GO:0007155 "cell adhesion" evidence=TAS] [GO:0007267 "cell-cell signaling" evidence=TAS] [GO:0007399 "nervous system development" evidence=TAS] [GO:0007411 "axon guidance" evidence=TAS] [GO:0008046 "axon guidance receptor activity" evidence=IEA] [GO:0016020 "membrane" evidence=ISS] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0021536 "diencephalon development" evidence=ISS] [GO:0030215 "semaphorin receptor binding" evidence=ISS] [GO:0030836 "positive regulation of actin filament depolymerization" evidence=ISS] [GO:0034260 "negative regulation of GTPase activity" evidence=IMP] [GO:0035373 "chondroitin sulfate proteoglycan binding" evidence=ISS] [GO:0035413 "positive regulation of catenin import into nucleus" evidence=ISS] [GO:0043395 "heparan sulfate proteoglycan binding" evidence=ISS] [GO:0045545 "syndecan binding" evidence=ISS] [GO:0045766 "positive regulation of angiogenesis" evidence=ISS] [GO:0048842 "positive regulation of axon extension involved in axon guidance" evidence=ISS] [GO:0048843 "negative regulation of axon extension involved in axon guidance" evidence=ISS] [GO:0051897 "positive regulation of protein kinase B signaling" evidence=ISS] [GO:0060326 "cell chemotaxis" evidence=ISS] [GO:2000352 "negative regulation of endothelial cell apoptotic process" evidence=ISS] [GO:2001028 "positive regulation of endothelial cell chemotaxis" evidence=ISS] InterPro:IPR001627 InterPro:IPR002165 InterPro:IPR015943 InterPro:IPR016201 Pfam:PF01403 Pfam:PF01437 PROSITE:PS51004 SMART:SM00423 SMART:SM00630 GO:GO:0016021 GO:GO:0005886 Reactome:REACT_111045 GO:GO:0007411 GO:GO:0007165 Gene3D:2.130.10.10 GO:GO:0034260 GO:GO:0001938 GO:GO:0007155 GO:GO:0007267 GO:GO:0045766 GO:GO:0002043 GO:GO:0001569 GO:GO:0051897 GO:GO:0043395 GO:GO:0060326 GO:GO:2000352 EMBL:CH471102 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 GO:GO:0035413 GO:GO:0045545 GO:GO:0030836 Orphanet:281 GO:GO:0048842 GO:GO:0008046 SUPFAM:SSF103575 GO:GO:2001028 SUPFAM:SSF101912 GO:GO:0048843 GO:GO:0030215 InterPro:IPR027231 PANTHER:PTHR11036 EMBL:U52840 EMBL:AC004615 EMBL:AC022446 EMBL:AC027336 EMBL:AC091906 EMBL:BC115696 PIR:JC5928 RefSeq:NP_003957.2 UniGene:Hs.27621 ProteinModelPortal:Q13591 SMR:Q13591 BioGrid:114501 IntAct:Q13591 STRING:9606.ENSP00000371936 PhosphoSite:Q13591 DMDM:109939725 PaxDb:Q13591 PRIDE:Q13591 Ensembl:ENST00000382496 GeneID:9037 KEGG:hsa:9037 UCSC:uc003jek.2 CTD:9037 GeneCards:GC05M009036 HGNC:HGNC:10736 HPA:HPA004632 MIM:609297 neXtProt:NX_Q13591 PharmGKB:PA35658 eggNOG:NOG316291 HOGENOM:HOG000047106 HOVERGEN:HBG062356 InParanoid:Q13591 KO:K06841 OMA:YSNAYFT OrthoDB:EOG7SN8C0 PhylomeDB:Q13591 TreeFam:TF329951 GeneWiki:SEMA5A GenomeRNAi:9037 NextBio:33851 PRO:PR:Q13591 ArrayExpress:Q13591 Bgee:Q13591 CleanEx:HS_SEMA5A Genevestigator:Q13591 GO:GO:0035373 GO:GO:0021536 Uniprot:Q13591)

HSP 1 Score: 87.8113 bits (216), Expect = 5.424e-16
Identity = 91/320 (28.44%), Postives = 129/320 (40.31%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKIL-ILARQGGSPCRGERKESRVCSTQQC--PHFV--GDVPDVNCPL-----------VNVIGATYANCDGRYKVTNNK--------VPWASERPV------------YKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPC 348
            W+ ++SWS CS +C  G Q R+R       R GG  C G+ +E R C+      PH    G  P   C             +   G   A C+  Y+  N           PW    PV            +++T K R    N   +G    +R  ++  S    SG  TD         G+  + +   + S           +V+  WS++  WS+CS  C  G + RKR+   P    GG  C G ++E ++CN    CPV+  WS +  W+ CSATCGGG     R+    A    G  CLG   E   C+T PC
Sbjct:  598 WTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVG---RQRIEMRYCSSDGTSGCSTD---------GLSGDFLRAGRYS---------AHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEYQECNI-LPCPVDGVWSCWSPWTKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQPC 895          

HSP 2 Score: 84.7297 bits (208), Expect = 4.266e-15
Identity = 46/116 (39.66%), Postives = 60/116 (51.72%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKT 355
            W+ +  WS CS +CG G Q R+R    P+  +GG+ C G   E R CN    CP +  W+ +G W  C+A CGGG Q      RRR   +G  C G   E +SC+TNPC      T
Sbjct:  598 WTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQA-----RRRICENGPDCAGCNVEYQSCNTNPCPELKKTT 708          

HSP 3 Score: 53.1434 bits (126), Expect = 1.887e-5
Identity = 58/218 (26.61%), Postives = 79/218 (36.24%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNN 453
            VD +WS +  W++CS +CG G   R R    P+   GG  C G   E   CN    CP +  WS +  WS C A+  G +   R+ I     GS   C G   E+R C  +  S F  +     SSS  +      + F  I            SS     +T    T        +   T     +    NT I N +N   K   +        NN
Sbjct:  840 VDGVWSCWSPWTKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNT-QPCPES--WSEWSDWSECEAS--GVQVRARQCILLFPMGSQ--CSGNTTESRPCVFD--SNFIPEVSVARSSSVEEKRCGEFNMFHMIAVG-------LSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSITNHINKLDKYDSVEAIKAFNKNN 1041          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:SEMA5A "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0001569 "patterning of blood vessels" evidence=IEA] [GO:0008046 "axon guidance receptor activity" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] InterPro:IPR001627 InterPro:IPR002165 InterPro:IPR015943 InterPro:IPR016201 Pfam:PF01403 Pfam:PF01437 PROSITE:PS51004 SMART:SM00423 SMART:SM00630 GO:GO:0016021 GO:GO:0007165 Gene3D:2.130.10.10 GO:GO:0001569 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 GO:GO:0008046 SUPFAM:SSF103575 SUPFAM:SSF101912 InterPro:IPR027231 PANTHER:PTHR11036 GeneTree:ENSGT00690000101974 CTD:9037 KO:K06841 OMA:YSNAYFT OrthoDB:EOG7SN8C0 TreeFam:TF329951 EMBL:AAEX03017160 EMBL:AAEX03017161 EMBL:AAEX03017162 RefSeq:XP_005639728.1 RefSeq:XP_005639729.1 RefSeq:XP_005639730.1 RefSeq:XP_005639731.1 RefSeq:XP_005639732.1 RefSeq:XP_005639733.1 RefSeq:XP_005639734.1 RefSeq:XP_005639735.1 RefSeq:XP_848441.3 Ensembl:ENSCAFT00000016056 GeneID:478622 KEGG:cfa:478622 Uniprot:F1PBV7)

HSP 1 Score: 87.4261 bits (215), Expect = 7.297e-16
Identity = 82/284 (28.87%), Postives = 121/284 (42.61%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPC 348
            W+ +  W  C+  C GG+Q R R       + G  C G   E + C+T  CP      P      VN+     ++  G Y+          +R  +++T K R    N   +G    +R  ++  S    SG  TD         G+  + +   + S           +V+  WS++  WS+CS  C  G + RKR+   P    GG  C G ++E ++CN    CPV+  WS +  WS CSATCGGG     R+    A    G  CLG   E   C+T+PC
Sbjct:  655 WTGWGPWERCTAQCGGGIQARRRTC-----ENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNI-----SDNGGHYE----------QR--FRYTCKARLPDPNLLEVG---RQRIEMRYCSSDGTSGCSTD---------GLSGDFLRAGRYS---------AHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEYQECNI-LPCPVDGVWSCWSPWSKCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEAVCNTDPC 894          

HSP 2 Score: 83.1889 bits (204), Expect = 1.399e-14
Identity = 56/158 (35.44%), Postives = 75/158 (47.47%), Query Frame = 0

Query:  200 DTDEPWEGKWE-EGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKT 355
            D+  P  G W+ EG R+E           A+ S + G     W+ +  WS CS +CG G Q R+R    P+  +GG+ C G   E R CN    CP +  W+ +G W  C+A CGGG Q      RRR   +G  C G   E + C+TNPC      T
Sbjct:  570 DSPAPQCGGWQCEGPRMEI----------ANCSRNGG-----WTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQA-----RRRTCENGPDCAGCNVEYQPCNTNPCPELKKTT 707          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:SEMA5B "Uncharacterized protein" species:9913 "Bos taurus" [GO:0004872 "receptor activity" evidence=IEA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] InterPro:IPR001627 InterPro:IPR002165 InterPro:IPR015943 InterPro:IPR016201 Pfam:PF01403 Pfam:PF01437 PROSITE:PS51004 SMART:SM00423 SMART:SM00630 GO:GO:0007275 GO:GO:0016020 Gene3D:2.130.10.10 GO:GO:0004872 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 SUPFAM:SSF103575 SUPFAM:SSF101912 InterPro:IPR027231 PANTHER:PTHR11036 GeneTree:ENSGT00690000101974 OrthoDB:EOG7SN8C0 TreeFam:TF329951 OMA:SRSRHCE EMBL:DAAA02001608 Ensembl:ENSBTAT00000024989 Uniprot:F1MTB6)

HSP 1 Score: 87.4261 bits (215), Expect = 7.698e-16
Identity = 88/285 (30.88%), Postives = 111/285 (38.95%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGL-DTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPC 348
            W+ + SWS CS +C GGVQ R R       + G+ C G   E + C+ + CP    + P      VNV     A  + R++ T  + P                        G   G+R           SG  DTD   E     G           SP   S           W+++G WS CS  C  G + RKR    P   NGG  C GDA E + CN  A CPV   WS +  WS CSA+CGGG     R+    A   G   CLG   E   C+T  C
Sbjct:  670 WASWGSWSKCSSNCGGGVQSRRRTC-----ENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGG-ARQEQRFRFTC-RAPLPDPH-------------------GLQFGRRRMETRTCPADGSGACDTDALVEDLLRSG---------GASPLMVSGG---------WAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHA-CPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQAC 909          

HSP 2 Score: 80.1073 bits (196), Expect = 1.128e-13
Identity = 47/129 (36.43%), Postives = 65/129 (50.39%), Query Frame = 0

Query:  235 GSVDCL--------------WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            G +DCL              W+ +  W+ CS SCG G Q R+R    P+  +GG+ C G + E R CN    CPV   W+++G WS CS+ CGGG Q+     RRR   +G  C G   E ++C+   C
Sbjct:  592 GGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQS-----RRRTCENGNSCPGCGVEFKTCNPEAC 715          

HSP 3 Score: 48.9062 bits (115), Expect = 4.488e-4
Identity = 21/51 (41.18%), Postives = 34/51 (66.67%), Query Frame = 0

Query:  466 WSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHT 515
            W+ + +W++CS  CG+G Q R RS  +P+ R+GG  C G++ + R CN +T
Sbjct:  612 WTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENT 662          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:SEMA5B "Semaphorin-5B" species:9606 "Homo sapiens" [GO:0004872 "receptor activity" evidence=IEA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] InterPro:IPR001627 InterPro:IPR002165 InterPro:IPR015943 InterPro:IPR016201 Pfam:PF01403 Pfam:PF01437 PROSITE:PS51004 SMART:SM00423 SMART:SM00630 GO:GO:0007275 GO:GO:0016020 Gene3D:2.130.10.10 GO:GO:0004872 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 SUPFAM:SSF103575 SUPFAM:SSF101912 InterPro:IPR027231 PANTHER:PTHR11036 HOGENOM:HOG000047106 EMBL:AC078794 EMBL:AC083797 EMBL:AC109130 HGNC:HGNC:10737 ProteinModelPortal:C9JKR3 SMR:C9JKR3 Ensembl:ENST00000195173 NextBio:35486857 ArrayExpress:C9JKR3 Bgee:C9JKR3 Uniprot:C9JKR3)

HSP 1 Score: 86.6557 bits (213), Expect = 1.061e-15
Identity = 96/307 (31.27%), Postives = 125/307 (40.72%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPCSRF------------GAKTESRH 359
            W+ + SWS CS +C GG+Q R R     A + G+ C G   E + C+ + CP    + P      VNV     A  + R++ T  + P A    +    R+       A G G                    DTD            VE +        S STS  T  V   W+++G WS CS  C  G + RKR    P   NGG  C GDA E + CN  A CPV   WS +  WS CSA+CGGG     R+    A   G   CLG   E   C+T  C  +            GA++ SRH
Sbjct:  725 WASWGSWSKCSSNCGGGMQSRRR-----ACENGNSCLGC-GEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGG-ARQEQRFRFTC-RAPLADPHGLQFGRRRTETRTCPADGSG------------------SCDTD----------ALVEVLL------RSGSTSPHT--VSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQA-CPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPGWSPWSEWSKCTDDGAQSRSRH 986          

HSP 2 Score: 80.1073 bits (196), Expect = 1.044e-13
Identity = 49/129 (37.98%), Postives = 67/129 (51.94%), Query Frame = 0

Query:  235 GSVDCL--------------WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            G +DCL              W+ +  W+ CS SCG G Q R+R    P+  +GG+ C G + E R CN    CPV   W+++G WS CS+ CGGG Q+     RRRA  +G  CLG   E ++C+   C
Sbjct:  647 GGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQS-----RRRACENGNSCLG-CGEFKTCNPEGC 769          

HSP 3 Score: 48.9062 bits (115), Expect = 3.984e-4
Identity = 21/51 (41.18%), Postives = 34/51 (66.67%), Query Frame = 0

Query:  466 WSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHT 515
            W+ + +W++CS  CG+G Q R RS  +P+ R+GG  C G++ + R CN +T
Sbjct:  667 WTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENT 717          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:F1MLA7 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0005179 "hormone activity" evidence=IEA] [GO:0005576 "extracellular region" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0030414 "peptidase inhibitor activity" evidence=IEA] InterPro:IPR000421 InterPro:IPR001007 InterPro:IPR001545 InterPro:IPR002172 InterPro:IPR008037 Pfam:PF00057 Pfam:PF00093 Pfam:PF00754 PRINTS:PR00261 PROSITE:PS01208 PROSITE:PS01285 PROSITE:PS01286 PROSITE:PS50022 PROSITE:PS50068 PROSITE:PS50184 SMART:SM00068 SMART:SM00192 SMART:SM00214 SMART:SM00231 GO:GO:0005737 GO:GO:0005576 GO:GO:0030414 GO:GO:0007155 OrthoDB:EOG73V6J9 InterPro:IPR002919 Pfam:PF01826 SUPFAM:SSF57567 Gene3D:2.60.120.260 InterPro:IPR008979 SUPFAM:SSF49785 Gene3D:4.10.400.10 InterPro:IPR023415 SUPFAM:SSF57424 PROSITE:PS01209 InterPro:IPR001846 Pfam:PF00094 SMART:SM00216 PROSITE:PS51233 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR014853 Pfam:PF08742 SMART:SM00832 InterPro:IPR006207 PROSITE:PS01225 SMART:SM00041 GO:GO:0010466 OMA:SSCHKLV InterPro:IPR006552 SMART:SM00215 InterPro:IPR028504 PANTHER:PTHR22905 SUPFAM:SSF57283 GeneTree:ENSGT00730000110607 TreeFam:TF344272 EMBL:DAAA02011963 EMBL:DAAA02011959 EMBL:DAAA02011960 EMBL:DAAA02011961 EMBL:DAAA02011962 Ensembl:ENSBTAT00000043975 Uniprot:F1MLA7)

HSP 1 Score: 87.0409 bits (214), Expect = 1.157e-15
Identity = 76/290 (26.21%), Postives = 117/290 (40.34%), Query Frame = 0

Query:  231 SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN-IRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISS----SFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQ---PIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVH-PSRNGGNECPGRAFQSRIC 511
            SD+ G VDC  + +  WS CS SCG G   ++R +L+P   GG SC  D + S+ C   A CPV   W+ +  W  CS +CGGG ++ RR+ +       G PC G   E   C   PC+         H      +  ++      +F+  P++     TL   S         +      +   +       T      + P   E  A L +    P+P                   C WS + +W  C + CG G++ R RS  + P+ +GG  C G+  + + C
Sbjct: 2471 SDENGCVDCGLAPWSGWSSCSRSCGLGLAFQRRELLRPPLPGG-SCPPDRLRSQPCFVQA-CPVAGAWAEWEAWGPCSVSCGGGHRSRRRSCMDPPPKNGGAPCPGPPQERAPCGLQPCAGGTGAGAGGHLRGEPRVKGTVPGCPCWAFNPRPSAELNMLTLAWPSLPPFLPHPPSPAHRPPSAM-SGVVFHPHTIQQLFRHPPSVCEKGALLCEPGGCPVP-------------------CGWSAWSSWGPCDRSCGSGLRARFRSPSNPPAASGGAPCEGQRQELQAC 2738          

HSP 2 Score: 63.1586 bits (152), Expect = 2.496e-8
Identity = 67/264 (25.38%), Postives = 95/264 (35.98%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSC---GTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSF-----------STIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRS 489
            W+ +  WS CS SC   G G   R R  L P   G  SC G+A E   C+      +   W  +  WS+CSA C GG QT  R  R  AS  G P C G  ++TR C+T PC+          S+     +                              LF  +++    +            P +     + +  T     I  E+  A +  P+                    C WS +  WS+CS+ C VGV+ R R+
Sbjct: 2749 WTPWAPWSACSQSCLVPGGGPALRSRSRLCPGP-GDTSCIGEATEEEPCSPPVCLGLG-VWGQWAAWSACSAPCNGGVQT--RGRRCSASAPGDPGCQGPHSQTRDCNTQPCTAQCPGDMVFRSAEQCRWEGGPCPGLCLARGPGVECTGVCTAGCACPAGLFLHNSSCLPPSQCPCQLRGQLYAPGAVARLDSCSNCTC----ISGEMVCASEPCPV-------------------ACGWSPWTPWSLCSRSCNVGVRRRFRA 2985          

HSP 3 Score: 62.003 bits (149), Expect = 5.231e-8
Identity = 80/297 (26.94%), Postives = 110/297 (37.04%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQG-GSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYK--HTRKNRY-----IFWNAGGLGWSIGKREYLK--TGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIES------RQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTN 346
            W  +  WS CS+SC GG++ R R       QG G  C G R +   C    CP         NC    + GA Y+ C           P + +  V+   H +   Y     +    G +    G    L   TG   HR G    +P      +G           S    + +D    V   W  + +W+ CS  C   T+ R R    P+  +GG  C G+A E+        C +   CPV+  WS +G WS C   C G     R       S  GRPC G   ++R C  N
Sbjct: 3230 WGPWGPWSSCSRSCGGGLRSRSRACDQPPPQGLGDYCEGPRAQGAACQALPCP-------VTNC--TAIEGAEYSAC-------GPPCPRSCDDLVHCVWHCQPGCYCPPGQVLSADGTVCVQPGHCSCLDLLTGER-HRPGAQLAKP------DGCNY-----CTCSEGQLTCTDLPCPVPGAWCPWSEWTACSQPCQGQTRTRSRACSCPAPQHGGAPCPGEAGEAGAQHQRETCASTPECPVDGAWSPWGPWSPCD-VCLGQSHRSRECSWPPTSEGGRPCPGGHRQSRPCQGN 3497          

HSP 4 Score: 60.4622 bits (145), Expect = 1.331e-7
Identity = 45/116 (38.79%), Postives = 57/116 (49.14%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAI----ESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRAS-GSGRPCLGEKAETRSCSTNPC 348
            +C W+++  W  CS SCG G QQR+     P   GG  C  D +    E R CN  A CPV   WS +  WS C  +CGGGR    R+         G PC+GE+   R C+  PC
Sbjct: 3933 NCSWTAWAPWEPCSRSCGVG-QQRRLRAYHPPGPGGHWCP-DVLTAYQERRFCNLRA-CPVPGGWSRWSPWSWCDRSCGGGRSLRSRSCSSPPPKNGGAPCVGERHHARLCNPTPC 4045          

HSP 5 Score: 54.6842 bits (130), Expect = 9.143e-6
Identity = 67/290 (23.10%), Postives = 97/290 (33.45%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKS-------------CRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGG-RQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC------SRFGAKTESRHSSSNSDIDNSISSSFSTIPTS-SPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQS 508
            WS +  WS CS SCG   + + R   +P      S             C G   E   C     C     W  +G WSSCS +CGGG R   R   +    G G  C G +A+  +C   PC      +  GA+  +         D+ +   +   P    P    L   S   T        +     T       +        N    +E      D P P                     W  +  W+ CS+PC    + R+R+   P+ ++GG  CPG A ++
Sbjct: 3161 WSPWTPWSECSASCGPARRHKHRFCTRPPGGAPSSMAPPLLLSSVPPLCPGPEAEEEPCLL-PECDRAGGWGPWGPWSSCSRSCGGGLRSRSRACDQPPPQGLGDYCEGPRAQGAACQALPCPVTNCTAIEGAEYSACGPPCPRSCDDLVHCVWHCQPGCYCPPGQVL---SADGTVCVQPGHCSCLDLLTGERHRPGAQLAKPDGCNYCTCSEGQLTCTDLPCPVPG-----------------AWCPWSEWTACSQPCQGQTRTRSRACSCPAPQHGGAPCPGEAGEA 3429          

HSP 6 Score: 54.299 bits (129), Expect = 9.945e-6
Identity = 72/293 (24.57%), Postives = 107/293 (36.52%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHS-SSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTT------TPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDD-DLSTTNNSRSSIKRVQ-------DCEWSDYDTWSVCSKPCGV-GVQYRNRSIVHPSRNG-GNECPGRAFQSRICNRHTC 516
            W  +  W  CS +CG G Q R R    PS      C G   ++R C A A CP +  W+++ +WS CS  CGG   T R           R C   +   R+C+T P    G    +R +     D   +++ S   +      +    P   T   I+   T              P      +          P   + +     Q +  D  L    + R   +R Q       +C WS +  W+ C  PCG   VQ+  RS  +P   G G++C G   ++R C    C
Sbjct: 1693 WGPWSPWELCSRTCGPGVQGRSRRCSPPSLPVLWHCPGPERQTRACFA-AACPEDGAWTSWSRWSPCSEPCGG--VTARH----------RECHPPQNGGRTCATLP----GGPPSTRETRPCPQDGCPNVTCSGELV------FHACVPCPLTCDDISGQATCPPDRPCGGPGCWCPAGQVLGAQGRCVWPRQCPCLVDGSRYWPGQRVKTDCQLCVCQDGRP--RRCQPSPDCAVNCGWSAWSPWAECLGPCGSRSVQWSFRSPNNPRPAGRGHQCRGLHRKARRCQTEPC 1960          

HSP 7 Score: 51.6026 bits (122), Expect = 7.113e-5
Identity = 43/124 (34.68%), Postives = 57/124 (45.97%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQ-PSTNGGKSCRGDAIESRQCNA--GATCPVNCEWSNFGQWSSCSATC---GGGRQTXRRNIRRRASGSG-RPCLGEKAETRSCSTNPCSRFGA 353
            V C WS++  W  C  SCG+G + R R     P+ +GG  C G   E + C +  GA  P    W+ +  WS+CS +C   GGG     R+  R   G G   C+GE  E   CS   C   G 
Sbjct: 2688 VPCGWSAWSSWGPCDRSCGSGLRARFRSPSNPPAASGGAPCEGQRQELQACYSACGAEVP---GWTPWAPWSACSQSCLVPGGGPALRSRS--RLCPGPGDTSCIGEATEEEPCSPPVCLGLGV 2806          

HSP 8 Score: 51.2174 bits (121), Expect = 8.926e-5
Identity = 78/327 (23.85%), Postives = 109/327 (33.33%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILI-LARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNG--GKSCR---------------------------GDAIESRQCNAGATCP--VNCE---------WSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPCSRF 351
            WS +S WS C +SC GG   R R       + GG+PC GER  +R+C+   C        +  CP    + +    C  R       +    ++   +  R         GG    +G  E      H    G    E          ++ C       P+            C WS +  WS CS SCG   QQ +    + ST+G     CR                           GD+     C   +  P  + CE         W+ +  WS C  +CGGG Q   R     A    G PCLG   +++ C   PC   
Sbjct: 3994 WSRWSPWSWCDRSCGGGRSLRSRSCSSPPPKNGGAPCVGERHHARLCNPTPC--------EEGCPAGMEVVSCANRCPRRCSDLQEGIVCQEDQACQQGCRCPEGSLEQDGGCV-PLGHCECTDAQGHSWAPGSQHQEACNNCTCRAGQLSCTAQPCPPPA-----------HCAWSRWSAWSPCSRSCGPAGQQSR---FRSSTSGSWAPECREEQSQSQPCPQSPCPPLCLQGTRPRSLGDSWLQDGCQQCSCTPEGIICEDAECAGLGAWTPWSPWSDCPVSCGGGNQVRTRVCVASAPPRGGSPCLGPDVQSQRCGLWPCPAL 4297          

HSP 9 Score: 50.0618 bits (118), Expect = 2.104e-4
Identity = 41/137 (29.93%), Postives = 66/137 (48.18%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRM-ILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFST 376
            W+ +  WS C VSCG G Q R R+ +      GG  C G  ++S++C     CP   +  ++G W  CS +CG G  +      R AS    PCL  +AE    ST+P      + +++   +   ++  + SS+S+
Sbjct: 4243 WTPWSPWSDCPVSCGGGNQVRTRVCVASAPPRGGSPCLGPDVQSQRCGL-WPCPALPDTCSWGPWGPCSRSCGPGLAS------RSAS---CPCLLAEAEPACNSTSP------RLDTQACYAGPCLEECVWSSWSS 4363          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:Hmcn1 "Protein Hmcn1" species:10116 "Rattus norvegicus" [GO:0005509 "calcium ion binding" evidence=IEA] InterPro:IPR000742 InterPro:IPR001881 InterPro:IPR007110 InterPro:IPR018097 Pfam:PF07645 PROSITE:PS01187 PROSITE:PS50026 PROSITE:PS50835 SMART:SM00179 InterPro:IPR000152 RGD:1564772 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0005509 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 PROSITE:PS00010 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR002035 SMART:SM00327 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR009030 SUPFAM:SSF57184 Gene3D:2.40.155.10 InterPro:IPR023413 InterPro:IPR009017 SUPFAM:SSF54511 OMA:VFHLDKK OrthoDB:EOG7SXW2B InterPro:IPR006605 Pfam:PF07474 PROSITE:PS50993 GeneTree:ENSGT00730000110638 EMBL:AABR06075391 EMBL:AABR06075392 EMBL:AABR06075393 EMBL:AABR06075394 EMBL:AABR06075395 EMBL:AABR06075396 EMBL:AABR06075397 EMBL:AABR06075398 EMBL:AABR06075399 EMBL:AABR06075400 EMBL:AABR06075401 EMBL:AABR06075402 EMBL:AABR06075403 EMBL:AABR06075404 ProteinModelPortal:F1M4Q3 Ensembl:ENSRNOT00000030971 NextBio:35581302 Uniprot:F1M4Q3)

HSP 1 Score: 87.0409 bits (214), Expect = 1.238e-15
Identity = 49/116 (42.24%), Postives = 60/116 (51.72%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            LWS+   W  CSV+CG G Q+R R+   P   NGG+ C+G   E+RQC     CPV+  WS +  W  CS +CG G QT  R      A   GRPC G   ET  C+  PC   G 
Sbjct: 4533 LWSA---WRSCSVTCGKGIQKRSRLCNNPPPANGGRPCQGSESEARQCQ-NKLCPVDGHWSEWSFWEECSRSCGHGNQTRTRTCSNPPAQHGGRPCEGYAVETIMCNIRPCPVHGV 4644          

HSP 2 Score: 71.2478 bits (173), Expect = 6.921e-11
Identity = 50/156 (32.05%), Postives = 74/156 (47.44%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTT 394
            WS +  W  CS +C  G  +R R    P  +NGG++C G   + ++CN    CPV+  W  +  WS CS +CGGG +T +R       S SGR C G+  +   C+   C     +       + +DI+  I+   +TI T SP   T    +T T
Sbjct: 4759 WSPWSGWGMCSRTCNGGQMRRYRTCDNPRPSNGGRACGGPDTQIQRCNTD-MCPVDGSWGTWHSWSHCSVSCGGGERTRKRLCDNPVPSKSGRSCPGDATQVSRCNMQACPGGPQRARGSVIGNINDIEFGIAFLNATI-TDSPNSDTRIIQATIT 4912          

HSP 3 Score: 69.707 bits (169), Expect = 2.247e-10
Identity = 43/114 (37.72%), Postives = 57/114 (50.00%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPC 348
            VD  WS +  W  CS SCG G Q R R    P + +GG+ C G A+E+  CN    CPV+  W+ +  W SCS +CG G QT  R       S  G  C G + + + C+   C
Sbjct: 4584 VDGHWSEWSFWEECSRSCGHGNQTRTRTCSNPPAQHGGRPCEGYAVETIMCNI-RPCPVHGVWNAWQPWGSCSKSCGKGSQTRTRLCNSPPPSFGGAYCNGAETQMQVCNERHC 4696          

HSP 4 Score: 68.5514 bits (166), Expect = 5.256e-10
Identity = 38/118 (32.20%), Postives = 61/118 (51.69%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPCSRFG 352
            V  +W+++  W  CS SCG G+Q R R+   P  + GG  C G   + + CN    CPV+ +W+ +  WS+C+ +CGGG +   R+         G  C G   ++  C+++PC   G
Sbjct: 4641 VHGVWNAWQPWGSCSKSCGKGSQTRTRLCNSPPPSFGGAYCNGAETQMQVCNE-RHCPVDGKWAAWTSWSTCTVSCGGGTRKRTRDCSDPVPQYGGNKCEGTGVQSDFCNSDPCPTHG 4757          

BLAST of EMLSAG00000000316 vs. GO
Match: - (symbol:Hmcn1 "hemicentin 1" species:10116 "Rattus norvegicus" [GO:0005509 "calcium ion binding" evidence=IEA] InterPro:IPR000742 InterPro:IPR001881 InterPro:IPR007110 InterPro:IPR018097 Pfam:PF07645 PROSITE:PS01187 PROSITE:PS50026 PROSITE:PS50835 SMART:SM00179 InterPro:IPR000152 RGD:1564772 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0005509 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 PROSITE:PS00010 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR002035 SMART:SM00327 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR009030 SUPFAM:SSF57184 Gene3D:2.40.155.10 InterPro:IPR023413 InterPro:IPR009017 SUPFAM:SSF54511 OMA:VFHLDKK OrthoDB:EOG7SXW2B InterPro:IPR006605 Pfam:PF07474 PROSITE:PS50993 GeneTree:ENSGT00730000110638 EMBL:AABR06075391 EMBL:AABR06075392 EMBL:AABR06075393 EMBL:AABR06075394 EMBL:AABR06075395 EMBL:AABR06075396 EMBL:AABR06075397 EMBL:AABR06075398 EMBL:AABR06075399 EMBL:AABR06075400 EMBL:AABR06075401 EMBL:AABR06075402 EMBL:AABR06075403 EMBL:AABR06075404 ProteinModelPortal:F1M4Q3 Ensembl:ENSRNOT00000030971 NextBio:35581302 Uniprot:F1M4Q3)

HSP 1 Score: 87.0409 bits (214), Expect = 1.238e-15
Identity = 49/116 (42.24%), Postives = 60/116 (51.72%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            LWS+   W  CSV+CG G Q+R R+   P   NGG+ C+G   E+RQC     CPV+  WS +  W  CS +CG G QT  R      A   GRPC G   ET  C+  PC   G 
Sbjct: 4533 LWSA---WRSCSVTCGKGIQKRSRLCNNPPPANGGRPCQGSESEARQCQ-NKLCPVDGHWSEWSFWEECSRSCGHGNQTRTRTCSNPPAQHGGRPCEGYAVETIMCNIRPCPVHGV 4644          

HSP 2 Score: 71.2478 bits (173), Expect = 6.921e-11
Identity = 50/156 (32.05%), Postives = 74/156 (47.44%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTT 394
            WS +  W  CS +C  G  +R R    P  +NGG++C G   + ++CN    CPV+  W  +  WS CS +CGGG +T +R       S SGR C G+  +   C+   C     +       + +DI+  I+   +TI T SP   T    +T T
Sbjct: 4759 WSPWSGWGMCSRTCNGGQMRRYRTCDNPRPSNGGRACGGPDTQIQRCNTD-MCPVDGSWGTWHSWSHCSVSCGGGERTRKRLCDNPVPSKSGRSCPGDATQVSRCNMQACPGGPQRARGSVIGNINDIEFGIAFLNATI-TDSPNSDTRIIQATIT 4912          

HSP 3 Score: 69.707 bits (169), Expect = 2.247e-10
Identity = 43/114 (37.72%), Postives = 57/114 (50.00%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPC 348
            VD  WS +  W  CS SCG G Q R R    P + +GG+ C G A+E+  CN    CPV+  W+ +  W SCS +CG G QT  R       S  G  C G + + + C+   C
Sbjct: 4584 VDGHWSEWSFWEECSRSCGHGNQTRTRTCSNPPAQHGGRPCEGYAVETIMCNI-RPCPVHGVWNAWQPWGSCSKSCGKGSQTRTRLCNSPPPSFGGAYCNGAETQMQVCNERHC 4696          

HSP 4 Score: 68.5514 bits (166), Expect = 5.256e-10
Identity = 38/118 (32.20%), Postives = 61/118 (51.69%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPCSRFG 352
            V  +W+++  W  CS SCG G+Q R R+   P  + GG  C G   + + CN    CPV+ +W+ +  WS+C+ +CGGG +   R+         G  C G   ++  C+++PC   G
Sbjct: 4641 VHGVWNAWQPWGSCSKSCGKGSQTRTRLCNSPPPSFGGAYCNGAETQMQVCNE-RHCPVDGKWAAWTSWSTCTVSCGGGTRKRTRDCSDPVPQYGGNKCEGTGVQSDFCNSDPCPTHG 4757          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905133|gb|GAXK01053242.1| (TSA: Calanus finmarchicus comp354353_c4_seq115 transcribed RNA sequence)

HSP 1 Score: 234.572 bits (597), Expect = 5.912e-66
Identity = 129/249 (51.81%), Postives = 160/249 (64.26%), Query Frame = 0

Query:  749 AKPIDP-NRRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNGYDGGSPVNLKXXXXXXXLRLPQSQTERP----------RILCHQCGSLFSGSG-NPPC---EEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
             K + P N RV+C+ CGSLFSTDAP+C  F+    QQ  TC  GEACL+YS+ KS  D ++IRECF TSILLGSI++P+ P  EC P  VE+ D+I ACIC +DFCNG  G   V+   +   +  R P + TER           R+ CHQCGSLFS S  NP C   +E +  +  YC+ GEACLWYSW+KS+  TS IRECFS SILLGSID PL     C PQDI ++  S I ACLC ++ CN+
Sbjct:    8 VKTVQPKNARVQCWVCGSLFSTDAPDCPSFNKQDPQQLKTCDIGEACLYYSWKKSNTDTSVIRECFPTSILLGSIDDPVEPQQECNPASVES-DTINACICTSDFCNGI-GDDNVDKSPSLPTSKSRPPPTTTERSKEISTRSSLDRVQCHQCGSLFSSSSNNPDCDVFDESDPLQQNYCQPGEACLWYSWQKSSRSTSFIRECFSTSILLGSIDSPLAPSAYCEPQDISDSSLSKISACLCHSDLCNS 748          

HSP 2 Score: 165.236 bits (417), Expect = 3.225e-42
Identity = 104/282 (36.88%), Postives = 145/282 (51.42%), Query Frame = 0

Query:  757 RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDD--SIMACICATDFCNGYDG----------------GSPVNLKXXXXXXXLRL-----------------------------PQSQT--------ERPRILCHQCGSLFSGSGNPPC---EEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTE 978
            RV+C  CGSLFS+ +  P+CD FD S   QQ  C  GEACLWYS+ KS+   + IRECFSTSILLGSI++P+APS  C P+ + +     I AC+C +D CN Y G                 +P+ L  T     L L                             P++++        ++P + C+ CGSL   + N  C   +  +  + + C   EACL YSWKKS+ ET+ +RECF   +LLGSI  PL     C  +DI +  + +I+ACLC+T+
Sbjct:  440 RVQCHQCGSLFSSSSNNPDCDVFDESDPLQQNYCQPGEACLWYSWQKSSRSTSFIRECFSTSILLGSIDSPLAPSAYCEPQDISDSSLSKISACLCHSDLCNSYRGEGENKPELLPVHILTQNAPIPLNPTRGKQTLVLEEEPRQSLSGNTKKSARNGLAETNKSTRNPETKSVAKQSFHPDQPGLQCYSCGSLL--NPNKKCDTFDREDKTQVQTCLRDEACLMYSWKKSSTETATLRECFPTRVLLGSIQDPLVPLPTCSQRDITDDGSGSIFACLCNTD 1279          

HSP 3 Score: 94.3597 bits (233), Expect = 3.539e-19
Identity = 51/110 (46.36%), Postives = 68/110 (61.82%), Query Frame = 0

Query:  879 QTERPRILCHQCGSLFSGSGNPPCEEGN---AGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANGE 985
            Q +  R+ C  CGSLFS    P C   N     + + C  GEACL+YSWKKSN +TS+IRECF  SILLGSID P++ ++EC P  +    +  I AC+C ++ CN  G+
Sbjct:   20 QPKNARVQCWVCGSLFSTDA-PDCPSFNKQDPQQLKTCDIGEACLYYSWKKSNTDTSVIRECFPTSILLGSIDDPVEPQQECNPASV---ESDTINACICTSDFCNGIGD 337          

HSP 4 Score: 35.8094 bits (81), Expect = 1.028e+0
Identity = 16/32 (50.00%), Postives = 20/32 (62.50%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCN 1107
            PL P+  C  +DIS+   S I ACLC +D CN
Sbjct:  650 PLAPSAYCEPQDISDSSLSKISACLCHSDLCN 745          

HSP 5 Score: 33.4982 bits (75), Expect = 5.529e+0
Identity = 16/29 (55.17%), Postives = 20/29 (68.97%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTD 1104
            PL+P   C+ RDI++  S SI ACLC TD
Sbjct: 1193 PLVPLPTCSQRDITDDGSGSIFACLCNTD 1279          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905187|gb|GAXK01053188.1| (TSA: Calanus finmarchicus comp354353_c4_seq61 transcribed RNA sequence)

HSP 1 Score: 244.973 bits (624), Expect = 7.325e-66
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.157 bits (544), Expect = 5.213e-56
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 87.4261 bits (215), Expect = 2.121e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 4 Score: 77.411 bits (189), Expect = 2.282e-13
Identity = 47/80 (58.75%), Postives = 52/80 (65.00%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLG 820
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLG 2798          

HSP 5 Score: 69.3218 bits (168), Expect = 8.355e-11
Identity = 39/66 (59.09%), Postives = 49/66 (74.24%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLG 945
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLG 2798          

HSP 6 Score: 62.003 bits (149), Expect = 1.292e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 7 Score: 56.6102 bits (135), Expect = 5.675e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 8 Score: 49.6766 bits (117), Expect = 7.057e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 9 Score: 45.0542 bits (105), Expect = 2.238e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 10 Score: 44.2838 bits (103), Expect = 2.985e-3
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCNAGATCPVNC 296
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN  +    +C
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKASC 1124          

HSP 11 Score: 40.4318 bits (93), Expect = 5.225e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 12 Score: 33.8834 bits (76), Expect = 4.525e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905242|gb|GAXK01053133.1| (TSA: Calanus finmarchicus comp354353_c4_seq6 transcribed RNA sequence)

HSP 1 Score: 244.588 bits (623), Expect = 3.601e-65
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.542 bits (545), Expect = 1.260e-55
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 159.458 bits (402), Expect = 1.803e-38
Identity = 103/278 (37.05%), Postives = 142/278 (51.08%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEND--DSIMACICATDFCNGYDGGSP---------------VNLKXXXXXXXLRLP-----------------QSQTERPRILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG I++P+     C P+ +  D   +I+AC+C +D CN   G                     +       +R P                 + Q ++  + C+ CGSL   + + PCE+    ++ + + C  GEACL Y+W +S  + + +RECF  S+LLGSI  PL     C  +DI ++   +I ACLC T+ CN
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNSGRGNKEEPKDDTQQHKLQQEQRQPRPQIENKTIRTPIEPTPSYVTQTSHQGSRRPQPDQAGLQCYSCGSLLDPNAD-PCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCN 3389          

HSP 4 Score: 107.071 bits (266), Expect = 2.451e-22
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG ID PL+++  C P+DI E   S I ACLC+++ CN+
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNS 2909          

HSP 5 Score: 102.834 bits (255), Expect = 4.185e-21
Identity = 49/104 (47.12%), Postives = 68/104 (65.38%), Query Frame = 0

Query:  758 VKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEN--DDSIMACICATDFCNGYDGG 859
            ++C++CGSL   +A  C++FD S S+Q  TC+ GEACL Y++ +S    A +RECF TS+LLGSI  P+ P+  C  R + +  D SI AC+C TDFCN    G
Sbjct: 3093 LQCYSCGSLLDPNADPCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDDQNG 3404          

HSP 6 Score: 87.4261 bits (215), Expect = 2.460e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 7 Score: 62.3882 bits (150), Expect = 1.204e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 8 Score: 56.9954 bits (136), Expect = 5.333e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 9 Score: 49.6766 bits (117), Expect = 7.266e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 10 Score: 49.6766 bits (117), Expect = 7.710e-5
Identity = 22/36 (61.11%), Postives = 26/36 (72.22%), Query Frame = 0

Query: 1074 SFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDD 1109
            S PL+P T C+ RDI++    SI ACLC TDFCNDD
Sbjct: 3288 SAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDD 3395          

HSP 11 Score: 44.669 bits (104), Expect = 2.367e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 12 Score: 44.2838 bits (103), Expect = 3.264e-3
Identity = 22/55 (40.00%), Postives = 32/55 (58.18%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCN 287
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCN 1097          

HSP 13 Score: 40.4318 bits (93), Expect = 5.541e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 14 Score: 36.1946 bits (82), Expect = 1.151e+0
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLG 1111
            PL   + C  +DISE   S+I ACLC +D CN   G
Sbjct: 2811 PLKVKSSCEPKDISEDSESTILACLCESDLCNSGRG 2918          

HSP 15 Score: 33.8834 bits (76), Expect = 5.225e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905139|gb|GAXK01053236.1| (TSA: Calanus finmarchicus comp354353_c4_seq109 transcribed RNA sequence)

HSP 1 Score: 235.728 bits (600), Expect = 6.032e-65
Identity = 129/249 (51.81%), Postives = 160/249 (64.26%), Query Frame = 0

Query:  749 AKPIDP-NRRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNGYDGGSPVNLKXXXXXXXLRLPQSQTERP----------RILCHQCGSLFSGSG-NPPC---EEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
             K + P N RV+C+ CGSLFSTDAP+C  F+    QQ  TC  GEACL+YS+ KS  D ++IRECF TSILLGSI++P+ P  EC P  VE+ D+I ACIC +DFCNG  G   V+   +   +  R P + TER           R+ CHQCGSLFS S  NP C   +E +  +  YC+ GEACLWYSW+KS+  TS IRECFS SILLGSID PL     C PQDI ++  S I ACLC ++ CN+
Sbjct:    8 VKTVQPKNARVQCWVCGSLFSTDAPDCPSFNKQDPQQLKTCDIGEACLYYSWKKSNTDTSVIRECFPTSILLGSIDDPVEPQQECNPASVES-DTINACICTSDFCNGI-GDDNVDKSPSLPTSKSRPPPTTTERSKEISTRSSLDRVQCHQCGSLFSSSSNNPDCDVFDESDPLQQNYCQPGEACLWYSWQKSSRSTSFIRECFSTSILLGSIDSPLAPSAYCEPQDISDSSLSKISACLCHSDLCNS 748          

HSP 2 Score: 170.629 bits (431), Expect = 3.247e-43
Identity = 106/285 (37.19%), Postives = 147/285 (51.58%), Query Frame = 0

Query:  757 RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDD--SIMACICATDFCNGYDG----------------GSPVNLKXXXXXXXLRL-----------------------------PQSQT--------ERPRILCHQCGSLFSGSGNPPC---EEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            RV+C  CGSLFS+ +  P+CD FD S   QQ  C  GEACLWYS+ KS+   + IRECFSTSILLGSI++P+APS  C P+ + +     I AC+C +D CN Y G                 +P+ L  T     L L                             P++++        ++P + C+ CGSL   + N  C   +  +  + + C   EACL YSWKKS+ ET+ +RECF   +LLGSI  PL     C  +DI +  + +I+ACLC+T+ CN
Sbjct:  440 RVQCHQCGSLFSSSSNNPDCDVFDESDPLQQNYCQPGEACLWYSWQKSSRSTSFIRECFSTSILLGSIDSPLAPSAYCEPQDISDSSLSKISACLCHSDLCNSYRGEGENKPELLPVHILTQNAPIPLNPTRGKQTLVLEEEPRQSLSGNTKKSARNGLAETNKSTRNPETKSVAKQSFHPDQPGLQCYSCGSLL--NPNKKCDTFDREDKTQVQTCLRDEACLMYSWKKSSTETATLRECFPTRVLLGSIQDPLVPLPTCSQRDITDDGSGSIFACLCNTDFCN 1288          

HSP 3 Score: 110.538 bits (275), Expect = 6.151e-24
Identity = 70/196 (35.71%), Postives = 103/196 (52.55%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIE 1271
            PL+P   C+ RDI++  S SI ACLC TDFCNDD  ++  G  +     R  T+ + T    SP                RTTK +      ++ + P  R    D P   +  R    R +SCP+ + L   GCY IS+ R+GWIEA+K C+     LL+++ + E+Q ++E ++ S+ R R E+WL GNDI+ E
Sbjct: 1193 PLVPLPTCSQRDITDDGSGSIFACLCNTDFCNDD--KNLLGQTSKSNNGRVKTQQKTTRQPQSP---------------RRTTKRTI---PTTKRTFPPRRNPQQDRPRPSKTERQQEFR-SSCPQDFDLVGGGCYLISNERVGWIEARKKCQQREAILLSLETDSEKQKVVEYVASSTRRRRSEYWLAGNDIDEE 1717          

HSP 4 Score: 93.9745 bits (232), Expect = 9.898e-19
Identity = 51/110 (46.36%), Postives = 68/110 (61.82%), Query Frame = 0

Query:  879 QTERPRILCHQCGSLFSGSGNPPCEEGN---AGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANGE 985
            Q +  R+ C  CGSLFS    P C   N     + + C  GEACL+YSWKKSN +TS+IRECF  SILLGSID P++ ++EC P  +    +  I AC+C ++ CN  G+
Sbjct:   20 QPKNARVQCWVCGSLFSTDA-PDCPSFNKQDPQQLKTCDIGEACLYYSWKKSNTDTSVIRECFPTSILLGSIDDPVEPQQECNPASV---ESDTINACICTSDFCNGIGD 337          

HSP 5 Score: 36.1946 bits (82), Expect = 8.091e-1
Identity = 16/32 (50.00%), Postives = 20/32 (62.50%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCN 1107
            PL P+  C  +DIS+   S I ACLC +D CN
Sbjct:  650 PLAPSAYCEPQDISDSSLSKISACLCHSDLCN 745          

HSP 6 Score: 33.113 bits (74), Expect = 9.175e+0
Identity = 17/44 (38.64%), Postives = 26/44 (59.09%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCN----DDLGRSPG 1115
            P+ P   CN   +   +S +I AC+CT+DFCN    D++ +SP 
Sbjct:  239 PVEPQQECNPASV---ESDTINACICTSDFCNGIGDDNVDKSPS 361          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905243|gb|GAXK01053132.1| (TSA: Calanus finmarchicus comp354353_c4_seq5 transcribed RNA sequence)

HSP 1 Score: 244.973 bits (624), Expect = 6.825e-65
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.157 bits (544), Expect = 2.429e-55
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 159.844 bits (403), Expect = 1.537e-38
Identity = 103/278 (37.05%), Postives = 142/278 (51.08%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEND--DSIMACICATDFCNGYDGGSP---------------VNLKXXXXXXXLRLP-----------------QSQTERPRILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG I++P+     C P+ +  D   +I+AC+C +D CN   G                     +       +R P                 + Q ++  + C+ CGSL   + + PCE+    ++ + + C  GEACL Y+W +S  + + +RECF  S+LLGSI  PL     C  +DI ++   +I ACLC T+ CN
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNSGRGNKEEPKDDTQQHKLQQEQRQPRPQIENKTIRTPIEPTPSYVTQTSHQGSRRPQPDQAGLQCYSCGSLLDPNAD-PCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCN 3389          

HSP 4 Score: 159.844 bits (403), Expect = 1.618e-38
Identity = 96/264 (36.36%), Postives = 130/264 (49.24%), Query Frame = 0

Query: 1074 SFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICEL 1337
            S PL+P T C+ RDI++    SI ACLC TDFCNDD        A S    + TT  +   TT+SP R             E +T+        ++    G                       SCP  ++L    CY IS  R+GWIEA+K+C+     L+++  EE+R  +  L+S  S R R E+WLGGNDIE E  W W      +  PD GW + P  S +ENCL W +T+      R S +GWH  SCCN+++YICE+
Sbjct: 3288 SAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDDQNARGDMGAQSSANTKPTTW-KPQSTTSSPWR-----------LVESSTRNPISKTSYTKGQQTG----------------------ASCPSEFSLVHGTCYFISYDRVGWIEARKMCQQRGAQLISLQTEEKRDRVRSLVSRLSKRRRSEYWLGGNDIEEEGVWEWASRRTPV--PDGGWSEEPYNSYDENCLAWMVTI---NGPRDSRDGWHGASCCNSLRYICEM 3962          

HSP 5 Score: 107.071 bits (266), Expect = 2.598e-22
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG ID PL+++  C P+DI E   S I ACLC+++ CN+
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNS 2909          

HSP 6 Score: 87.4261 bits (215), Expect = 2.528e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 7 Score: 62.003 bits (149), Expect = 1.326e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 8 Score: 56.6102 bits (135), Expect = 6.146e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 9 Score: 49.6766 bits (117), Expect = 7.771e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 10 Score: 44.669 bits (104), Expect = 2.651e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 11 Score: 44.2838 bits (103), Expect = 3.562e-3
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCNAGATCPVNC 296
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN  +    +C
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKASC 1124          

HSP 12 Score: 40.4318 bits (93), Expect = 5.631e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 13 Score: 36.1946 bits (82), Expect = 1.010e+0
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLG 1111
            PL   + C  +DISE   S+I ACLC +D CN   G
Sbjct: 2811 PLKVKSSCEPKDISEDSESTILACLCESDLCNSGRG 2918          

HSP 14 Score: 33.8834 bits (76), Expect = 5.104e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905245|gb|GAXK01053130.1| (TSA: Calanus finmarchicus comp354353_c4_seq3 transcribed RNA sequence)

HSP 1 Score: 244.973 bits (624), Expect = 7.139e-65
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.157 bits (544), Expect = 2.514e-55
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 159.844 bits (403), Expect = 1.566e-38
Identity = 103/278 (37.05%), Postives = 142/278 (51.08%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEND--DSIMACICATDFCNGYDGGSP---------------VNLKXXXXXXXLRLP-----------------QSQTERPRILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG I++P+     C P+ +  D   +I+AC+C +D CN   G                     +       +R P                 + Q ++  + C+ CGSL   + + PCE+    ++ + + C  GEACL Y+W +S  + + +RECF  S+LLGSI  PL     C  +DI ++   +I ACLC T+ CN
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNSGRGNKEEPKDDTQQHKLQQEQRQPRPQIENKTIRTPIEPTPSYVTQTSHQGSRRPQPDQAGLQCYSCGSLLDPNAD-PCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCN 3389          

HSP 4 Score: 158.688 bits (400), Expect = 3.798e-38
Identity = 96/263 (36.50%), Postives = 129/263 (49.05%), Query Frame = 0

Query: 1074 SFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICE 1336
            S PL+P T C+ RDI++    SI ACLC TDFCNDD        A S    + TT  +   TT+SP R             E +T+        ++    G                       SCP  ++L    CY IS  R+GWIEA+K+C+     L+++  EE+R  +  L+S  S R R E+WLGGNDIE E  W W      +  PD GW + P  S +ENCL W +T+      R S +GWH  SCCN+++YICE
Sbjct: 3288 SAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDDQNARGDMGAQSSANTKPTTW-KPQSTTSSPWR-----------LVESSTRNPISKTSYTKGQQTG----------------------ASCPSEFSLVHGTCYFISYDRVGWIEARKMCQQRGAQLISLQTEEKRDRVRSLVSRLSKRRRSEYWLGGNDIEEEGVWEWASRRTPV--PDGGWSEEPYNSYDENCLAWMVTI---NGPRDSRDGWHGASCCNSLRYICE 3959          

HSP 5 Score: 107.071 bits (266), Expect = 2.621e-22
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG ID PL+++  C P+DI E   S I ACLC+++ CN+
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNS 2909          

HSP 6 Score: 87.4261 bits (215), Expect = 2.544e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 7 Score: 62.003 bits (149), Expect = 1.332e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 8 Score: 56.6102 bits (135), Expect = 6.167e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 9 Score: 49.6766 bits (117), Expect = 7.793e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 10 Score: 44.669 bits (104), Expect = 2.657e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 11 Score: 44.2838 bits (103), Expect = 3.570e-3
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCNAGATCPVNC 296
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN  +    +C
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKASC 1124          

HSP 12 Score: 40.4318 bits (93), Expect = 5.642e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 13 Score: 36.1946 bits (82), Expect = 1.011e+0
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLG 1111
            PL   + C  +DISE   S+I ACLC +D CN   G
Sbjct: 2811 PLKVKSSCEPKDISEDSESTILACLCESDLCNSGRG 2918          

HSP 14 Score: 33.8834 bits (76), Expect = 5.112e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905244|gb|GAXK01053131.1| (TSA: Calanus finmarchicus comp354353_c4_seq4 transcribed RNA sequence)

HSP 1 Score: 244.973 bits (624), Expect = 7.288e-65
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.157 bits (544), Expect = 2.848e-55
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 159.844 bits (403), Expect = 1.790e-38
Identity = 103/278 (37.05%), Postives = 142/278 (51.08%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEND--DSIMACICATDFCNGYDGGSP---------------VNLKXXXXXXXLRLP-----------------QSQTERPRILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG I++P+     C P+ +  D   +I+AC+C +D CN   G                     +       +R P                 + Q ++  + C+ CGSL   + + PCE+    ++ + + C  GEACL Y+W +S  + + +RECF  S+LLGSI  PL     C  +DI ++   +I ACLC T+ CN
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNSGRGNKEEPKDDTQQHKLQQEQRQPRPQIENKTIRTPIEPTPSYVTQTSHQGSRRPQPDQAGLQCYSCGSLLDPNAD-PCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCN 3389          

HSP 4 Score: 159.073 bits (401), Expect = 3.335e-38
Identity = 98/264 (37.12%), Postives = 128/264 (48.48%), Query Frame = 0

Query: 1074 SFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICEL 1337
            S PL+P T C+ RDI++    SI ACLC TDFCNDD        A S    + TT  +   TT+SP R             E +T+        ++    G                       SCP  ++L    CY IS  R+GWIEA+K+C+     L+++  EE+R  +  L+S  S R R E+WLGGNDIE E  W W      +  PD GW + P  S EENCL W+IT         S +GWH  SCCN ++YICEL
Sbjct: 3288 SAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDDQNARGDMGAQSSANTKPTTW-KPQSTTSSPWR-----------LVESSTRNPISKTSYTKGQQTG----------------------ASCPSEFSLVHGTCYFISYDRVGWIEARKMCQQRGAQLISLQTEEKRDRVRSLVSRLSKRRRSEYWLGGNDIEEEGVWEWASRRTPV--PDGGWSEEPYNSYEENCLAWTIT---NNGPSDSRDGWHGSSCCNNLRYICEL 3962          

HSP 5 Score: 107.071 bits (266), Expect = 2.826e-22
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG ID PL+++  C P+DI E   S I ACLC+++ CN+
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNS 2909          

HSP 6 Score: 87.4261 bits (215), Expect = 2.697e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 7 Score: 62.003 bits (149), Expect = 1.354e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 8 Score: 56.6102 bits (135), Expect = 6.164e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 9 Score: 49.6766 bits (117), Expect = 7.790e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 10 Score: 44.669 bits (104), Expect = 2.568e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 11 Score: 44.2838 bits (103), Expect = 3.509e-3
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCNAGATCPVNC 296
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN  +    +C
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKASC 1124          

HSP 12 Score: 40.4318 bits (93), Expect = 5.689e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 13 Score: 36.1946 bits (82), Expect = 1.011e+0
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLG 1111
            PL   + C  +DISE   S+I ACLC +D CN   G
Sbjct: 2811 PLKVKSSCEPKDISEDSESTILACLCESDLCNSGRG 2918          

HSP 14 Score: 33.8834 bits (76), Expect = 5.286e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905247|gb|GAXK01053128.1| (TSA: Calanus finmarchicus comp354353_c4_seq1 transcribed RNA sequence)

HSP 1 Score: 244.973 bits (624), Expect = 7.288e-65
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.157 bits (544), Expect = 2.556e-55
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 159.844 bits (403), Expect = 1.579e-38
Identity = 103/278 (37.05%), Postives = 142/278 (51.08%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEND--DSIMACICATDFCNGYDGGSP---------------VNLKXXXXXXXLRLP-----------------QSQTERPRILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG I++P+     C P+ +  D   +I+AC+C +D CN   G                     +       +R P                 + Q ++  + C+ CGSL   + + PCE+    ++ + + C  GEACL Y+W +S  + + +RECF  S+LLGSI  PL     C  +DI ++   +I ACLC T+ CN
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNSGRGNKEEPKDDTQQHKLQQEQRQPRPQIENKTIRTPIEPTPSYVTQTSHQGSRRPQPDQAGLQCYSCGSLLDPNAD-PCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCN 3389          

HSP 4 Score: 159.844 bits (403), Expect = 1.662e-38
Identity = 96/264 (36.36%), Postives = 130/264 (49.24%), Query Frame = 0

Query: 1074 SFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICEL 1337
            S PL+P T C+ RDI++    SI ACLC TDFCNDD        A S    + TT  +   TT+SP R             E +T+        ++    G                       SCP  ++L    CY IS  R+GWIEA+K+C+     L+++  EE+R  +  L+S  S R R E+WLGGNDIE E  W W      +  PD GW + P  S +ENCL W +T+      R S +GWH  SCCN+++YICE+
Sbjct: 3288 SAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDDQNARGDMGAQSSANTKPTTW-KPQSTTSSPWR-----------LVESSTRNPISKTSYTKGQQTG----------------------ASCPSEFSLVHGTCYFISYDRVGWIEARKMCQQRGAQLISLQTEEKRDRVRSLVSRLSKRRRSEYWLGGNDIEEEGVWEWASRRTPV--PDGGWSEEPYNSYDENCLAWMVTI---NGPRDSRDGWHGASCCNSLRYICEM 3962          

HSP 5 Score: 107.071 bits (266), Expect = 2.632e-22
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG ID PL+++  C P+DI E   S I ACLC+++ CN+
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNS 2909          

HSP 6 Score: 87.4261 bits (215), Expect = 2.552e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 7 Score: 62.003 bits (149), Expect = 1.334e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 8 Score: 56.6102 bits (135), Expect = 6.177e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 9 Score: 49.6766 bits (117), Expect = 7.803e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 10 Score: 44.669 bits (104), Expect = 2.660e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 11 Score: 44.2838 bits (103), Expect = 3.574e-3
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCNAGATCPVNC 296
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN  +    +C
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKASC 1124          

HSP 12 Score: 40.4318 bits (93), Expect = 5.648e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 13 Score: 36.1946 bits (82), Expect = 1.012e+0
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLG 1111
            PL   + C  +DISE   S+I ACLC +D CN   G
Sbjct: 2811 PLKVKSSCEPKDISEDSESTILACLCESDLCNSGRG 2918          

HSP 14 Score: 33.8834 bits (76), Expect = 5.116e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905246|gb|GAXK01053129.1| (TSA: Calanus finmarchicus comp354353_c4_seq2 transcribed RNA sequence)

HSP 1 Score: 244.588 bits (623), Expect = 9.100e-65
Identity = 164/491 (33.40%), Postives = 231/491 (47.05%), Query Frame = 0

Query:  440 DQPIPDDDLSTTNNSRSSI--KRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS----RNGG--NECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECF--------------NGETTTTTN--SNFGNXXXXXXXXXXXXXXXXXXXXXRNRPEGVN---------------CEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC---------NFFXXXXXXXXXXXXXXVVKAKPIDPN---------------------------RRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNG 855
            D+   +DD   T N+  ++       C WS +  W  CSK CG G Q+R R ++  S      GG   +C G    ++ CN H+C   +  CC  V + + G A+  Q  YL  Y++++D +NGR AY R+G   L++ +  ++     +WV+GP LGQ +AG+RN G   C+H     W+YA R GVW++DD +L VEC+              +   T   N  S+  N           T         + R E  N               C WS++G WS CSK+CG+G+Q R R    T     S C G  K+T+ CNV  C         +   T+  P      R     +P  PN                            R+ C+ACGSLFSTDAPEC  F+ +  +QQ  C  GEACL Y + +S+   ++IRECF TSILLGSI++PI+   EC P  VE+ + I AC+C    CNG
Sbjct:  855 DETTDEDDSGLTANTEWNVLAPLPPSCLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKAS--CCNSVRLVSTGPARTYQPSYLTIYSRVADDHNGRPAYVRQGNGSLFMYYAITK-----MWVVGPHLGQDMAGVRNTGDGECVHHLGGGWRYAGRHGVWEEDDTSLRVECYTFDDYYDYTSDYVDSDSDTDYVNSLSDTENNPSEYLDHNTDTKDNASDKPKQARNEEDNEIEDWVGNDQDLEGKCIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCAAEHKSQAVSALITSDTPNQKHVTRTPTLKRPSTPNPSLKYQTKILTTKRKLPSTTSRPRLKTERITCYACGSLFSTDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAVES-EGISACVCTNTLCNG 2303          

HSP 2 Score: 214.157 bits (544), Expect = 2.558e-55
Identity = 113/224 (50.45%), Postives = 142/224 (63.39%), Query Frame = 0

Query:  126 DVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGXXXXXXXXXXXXXXXXXVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            ++ CP + V GA +A+C+G Y+++N  V WA  RPVYKH  KNR IFWNAGGLGWSIGK  YL +GS WHRSGL   EPW+G+WE+ VRVEC G           +    +VDCLWS +  WS CS SCG G Q+RKR +LQ   +GG  C G   E R+C +   C V C WS FG WS CS +CG G  T R+ ++R+ +G G PC G + ET SC  + CS
Sbjct:  204 EITCPRLTVYGADFASCNGEYRLSNASVEWAPLRPVYKHIVKNRVIFWNAGGLGWSIGKAAYLSSGSFWHRSGLSAAEPWQGEWEKNVRVECAG----------EATGLPAVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQS-EPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 3 Score: 159.458 bits (402), Expect = 1.971e-38
Identity = 103/278 (37.05%), Postives = 142/278 (51.08%), Query Frame = 0

Query:  747 VKAKPIDPNR----RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVEND--DSIMACICATDFCNGYDGGSP---------------VNLKXXXXXXXLRLP-----------------QSQTERPRILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            VK +P DP R    RVKC  CGSLFS+ A  P CD FD S  QQQ  C  GEACLWYS+ +S    +IIREC S SILLG I++P+     C P+ +  D   +I+AC+C +D CN   G                     +       +R P                 + Q ++  + C+ CGSL   + + PCE+    ++ + + C  GEACL Y+W +S  + + +RECF  S+LLGSI  PL     C  +DI ++   +I ACLC T+ CN
Sbjct: 2562 VKPRP-DPIRSRSDRVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNSGRGNKEEPKDDTQQHKLQQEQRQPRPQIENKTIRTPIEPTPSYVTQTSHQGSRRPQPDQAGLQCYSCGSLLDPNAD-PCEQFDRSDSRQVKTCSTGEACLMYTWSQSQDQAATLRECFPTSVLLGSISAPLVPATHCHKRDITDSGDGSIRACLCLTDFCN 3389          

HSP 4 Score: 154.066 bits (388), Expect = 9.918e-37
Identity = 94/264 (35.61%), Postives = 125/264 (47.35%), Query Frame = 0

Query: 1074 SFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICEL 1337
            S PL+P T C+ RDI++    SI ACLC TDFCNDD        A S    + TT  +   TT+SP R             E +T+        ++    G                       SCP  ++L    CY IS  R+GWIEA+K+C+     L+++  EE+R  +  L+S  S R R E+WL GNDIE E  W W      +  P FGW + P  S EENCL W++       R    + WH  SCCN +KYICE+
Sbjct: 3288 SAPLVPATHCHKRDITDSGDGSIRACLCLTDFCNDDQNARGDMGAQSSANTKPTTW-KPQSTTSSPWR-----------LVESSTRNPISKTSYTKGQQTG----------------------ASCPSEFSLVHGTCYFISYDRVGWIEARKMCQQRGAQLISLQTEEKRDRVRSLVSRLSKRRRSEYWLAGNDIEDEGVWEWARLRTQV--PMFGWSEEPFDSPEENCLVWTV---ESQGRADGKDFWHGSSCCNNLKYICEI 3962          

HSP 5 Score: 107.071 bits (266), Expect = 2.688e-22
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSG-NPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            R+ CHQCGSLFS S  NP C+  ++    + +YC+ GEACLWYSW++S   +SIIREC SKSILLG ID PL+++  C P+DI E   S I ACLC+++ CN+
Sbjct: 2601 RVKCHQCGSLFSSSASNPTCDAFDSSDPQQQDYCQPGEACLWYSWQQSESSSSIIRECLSKSILLGPIDDPLKVKSSCEPKDISEDSESTILACLCESDLCNS 2909          

HSP 6 Score: 87.4261 bits (215), Expect = 2.816e-16
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 0

Query:  874 RLPQSQTERPR-----ILCHQCGSLFSGSGNPPCEEGNAG---KTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            +LP S T RPR     I C+ CGSLFS +  P C   N+    + + CK GEACL Y W++S+ +TS+IRECF  SILLGSID P+    EC P  +    +  I AC+C    CN 
Sbjct: 1968 KLP-STTSRPRLKTERITCYACGSLFS-TDAPECPTFNSADPKQQKLCKVGEACLRYEWRESSDQTSVIRECFPTSILLGSIDDPISTEDECRPVAV---ESEGISACVCTNTLCNG 2303          

HSP 7 Score: 62.3882 bits (150), Expect = 1.266e-8
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0

Query:  673 GVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNF 730
             V+C WS + +WS CS SCG G+Q R R+V  TP++GG  CVG  K+ R C  + C  
Sbjct:  504 AVDCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPCTV 677          

HSP 8 Score: 56.6102 bits (135), Expect = 5.962e-7
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0

Query:  668 RNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            ++ P  V C WS++G WS CS+SCG G+  R ++V      GGS C G+ ++T SC + +C+
Sbjct:  657 QSEPCTVPCTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNCS 842          

HSP 9 Score: 49.6766 bits (117), Expect = 7.534e-5
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DC WS +  WS CS  CG G+Q R R ++   ++GG EC G   + R C    C
Sbjct:  510 DCLWSGFSDWSECSSSCGEGLQERKRKVLQTPQDGGLECVGPVKEERKCQSEPC 671          

HSP 10 Score: 44.669 bits (104), Expect = 2.657e-3
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNA 288
            C+WS +G WS+CS +CG G Q R R  L         C+G   E++ CN 
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNV 1808          

HSP 11 Score: 44.2838 bits (103), Expect = 3.540e-3
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPS----TNGG--KSCRGDAIESRQCNAGATCPVNC 296
            CLWS++G W +CS  CG G Q R R++L  S      GG  + C G+  +++ CN  +    +C
Sbjct:  933 CLWSAWGAWDKCSKECGGGDQHRFRILLVGSDGEVAQGGVDEDCEGEQADTKDCNMHSCDKASC 1124          

HSP 12 Score: 40.4318 bits (93), Expect = 5.548e-2
Identity = 18/53 (33.96%), Postives = 26/53 (49.06%), Query Frame = 0

Query:  464 CEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C WS++  WS CS+ CG GV  R + +      GG+ C G   ++  C    C
Sbjct:  681 CTWSEFGPWSECSQSCGEGVMTRRKQVKRKETGGGSPCEGAEEETESCYISNC 839          

HSP 13 Score: 36.1946 bits (82), Expect = 1.037e+0
Identity = 17/36 (47.22%), Postives = 21/36 (58.33%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLG 1111
            PL   + C  +DISE   S+I ACLC +D CN   G
Sbjct: 2811 PLKVKSSCEPKDISEDSESTILACLCESDLCNSGRG 2918          

HSP 14 Score: 33.8834 bits (76), Expect = 5.515e+0
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 0

Query:  296 CEWSNFGQWSSCSATCXXXXXXXXXXXXXXXXXXXXPCLGEKAETRSCSTNPCS 349
            C WS FG WS CS TCG G Q   R        S   C G + ET+ C+ + C+
Sbjct: 1659 CIWSKFGPWSQCSKTCGKGLQIRIRYPLNTGDTSESVCKGRRKETKYCNVDGCA 1820          

BLAST of EMLSAG00000000316 vs. C. finmarchicus
Match: gi|592905152|gb|GAXK01053223.1| (TSA: Calanus finmarchicus comp354353_c4_seq96 transcribed RNA sequence)

HSP 1 Score: 234.958 bits (598), Expect = 7.903e-64
Identity = 128/249 (51.41%), Postives = 159/249 (63.86%), Query Frame = 0

Query:  749 AKPIDP-NRRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNGYDGGSPVNLKXXXXXXXLRLPQSQTERP----------RILCHQCGSLFSGSGNPP----CEEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
             K + P N RV+C+ CGSLFSTDAP+C  F+    QQ  TC  GEACL+YS+ KS  D ++IRECF TSILLGSI++P+ P  EC P  VE+ D+I ACIC +DFCNG  G   V+   +   +  R P + TER           R+ CHQCGSLFS S N P     +E +  +  YC+ GEACLWYSW+KS+  TS IRECFS SILLGSID PL     C PQDI ++  S I ACLC ++ CN+
Sbjct:    8 VKTVQPKNARVQCWVCGSLFSTDAPDCPSFNKQDPQQLKTCDIGEACLYYSWKKSNTDTSVIRECFPTSILLGSIDDPVEPQQECNPASVES-DTINACICTSDFCNGI-GDDNVDKSPSLPTSKSRPPPTTTERSKEISTRSSLDRVQCHQCGSLFSSSSNNPDCDVFDESDPLQQNYCQPGEACLWYSWQKSSRSTSFIRECFSTSILLGSIDSPLAPSAYCEPQDISDSSLSKISACLCHSDLCNS 748          

HSP 2 Score: 170.244 bits (430), Expect = 9.605e-43
Identity = 106/285 (37.19%), Postives = 147/285 (51.58%), Query Frame = 0

Query:  757 RVKCFACGSLFSTDA--PECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDD--SIMACICATDFCNGYDG----------------GSPVNLKXXXXXXXLRL-----------------------------PQSQT--------ERPRILCHQCGSLFSGSGNPPC---EEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCN 981
            RV+C  CGSLFS+ +  P+CD FD S   QQ  C  GEACLWYS+ KS+   + IRECFSTSILLGSI++P+APS  C P+ + +     I AC+C +D CN Y G                 +P+ L  T     L L                             P++++        ++P + C+ CGSL   + N  C   +  +  + + C   EACL YSWKKS+ ET+ +RECF   +LLGSI  PL     C  +DI +  + +I+ACLC+T+ CN
Sbjct:  440 RVQCHQCGSLFSSSSNNPDCDVFDESDPLQQNYCQPGEACLWYSWQKSSRSTSFIRECFSTSILLGSIDSPLAPSAYCEPQDISDSSLSKISACLCHSDLCNSYRGEGENKPELLPVHILTQNAPIPLNPTRGKQTLVLEEEPRQSLSGNTKKSARNGLAETNKSTRNPETKSVAKQSFHPDQPGLQCYSCGSLL--NPNKKCDTFDREDKTQVQTCLRDEACLMYSWKKSSTETATLRECFPTRVLLGSIQDPLVPLPTCSQRDITDDGSGSIFACLCNTDFCN 1288          

HSP 3 Score: 169.088 bits (427), Expect = 2.455e-42
Identity = 99/262 (37.79%), Postives = 141/262 (53.82%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICEL 1337
            PL+P   C+ RDI++  S SI ACLC TDFCNDD  ++  G  +     R  T+ + T    SP                RTTK +      ++ + P  R    D P   +  R    R +SCP+ + L   GCY IS+ R+GWIEA+K C+     LL+++ + E+Q ++E ++ S+ R R E+WLGGNDIE E  W W      +  PD GW + P  S +ENCL W +T+      R S +GWH  SCCN+++YICE+
Sbjct: 1193 PLVPLPTCSQRDITDDGSGSIFACLCNTDFCNDD--KNLLGQTSKSNNGRVKTQQKTTRQPQSP---------------RRTTKRTI---PTTKRTFPPRRNPQQDRPRPSKTERQQEFR-SSCPQDFDLVGGGCYLISNERVGWIEARKKCQQREAILLSLETDSEKQKVVEYVASSTRRRRSEYWLGGNDIEEEGVWEWASRRTPV--PDGGWSEEPYNSYDENCLAWMVTI---NGPRDSRDGWHGASCCNSLRYICEM 1900          

HSP 4 Score: 93.9745 bits (232), Expect = 1.602e-18
Identity = 49/109 (44.95%), Postives = 68/109 (62.39%), Query Frame = 0

Query:  879 QTERPRILCHQCGSLFSGSGN--PPCEEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANGE 985
            Q +  R+ C  CGSLFS      P   + +  + + C  GEACL+YSWKKSN +TS+IRECF  SILLGSID P++ ++EC P  +    +  I AC+C ++ CN  G+
Sbjct:   20 QPKNARVQCWVCGSLFSTDAPDCPSFNKQDPQQLKTCDIGEACLYYSWKKSNTDTSVIRECFPTSILLGSIDDPVEPQQECNPASV---ESDTINACICTSDFCNGIGD 337          

HSP 5 Score: 36.5798 bits (83), Expect = 8.251e-1
Identity = 16/32 (50.00%), Postives = 20/32 (62.50%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCN 1107
            PL P+  C  +DIS+   S I ACLC +D CN
Sbjct:  650 PLAPSAYCEPQDISDSSLSKISACLCHSDLCN 745          

HSP 6 Score: 32.7278 bits (73), Expect = 9.611e+0
Identity = 17/44 (38.64%), Postives = 26/44 (59.09%), Query Frame = 0

Query: 1076 PLLPTTGCNIRDISEPQSSSIEACLCTTDFCN----DDLGRSPG 1115
            P+ P   CN   +   +S +I AC+CT+DFCN    D++ +SP 
Sbjct:  239 PVEPQQECNPASV---ESDTINACICTSDFCNGIGDDNVDKSPS 361          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000000316 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1047:1859:17658:-1 gene:EMLSAG00000000316 transcript:EMLSAT00000000316 description:"maker-LSalAtl2s1047-snap-gene-0.10")

HSP 1 Score: 2714.1 bits (7034), Expect = 0.000e+0
Identity = 1338/1338 (100.00%), Postives = 1338/1338 (100.00%), Query Frame = 0

Query:    1 MEARVPRQSNVAKTSTRPTHKRLSRKPRRAELDGKTSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNFFTTTTRPPTTTTRRPVVKAKPIDPNRRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNGYDGGSPVNLKXTTTPTPLRLPQSQTERPRILCHQCGSLFSGSGNPPCEEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANGERSVESDSSSSSNDRFDSSQVNQEQNNNRNRNDNNSKNNQQTSRRPTTTRRPSFTQTTPSSQFRXPSTPPPRSQNTFSGGPGLQCYSCGSFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICELK 1338
            MEARVPRQSNVAKTSTRPTHKRLSRKPRRAELDGKTSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNFFTTTTRPPTTTTRRPVVKAKPIDPNRRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNGYDGGSPVNLKXTTTPTPLRLPQSQTERPRILCHQCGSLFSGSGNPPCEEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANGERSVESDSSSSSNDRFDSSQVNQEQNNNRNRNDNNSKNNQQTSRRPTTTRRPSFTQTTPSSQFRXPSTPPPRSQNTFSGGPGLQCYSCGSFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICELK
Sbjct:    1 MEARVPRQSNVAKTSTRPTHKRLSRKPRRAELDGKTSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCNFFTTTTRPPTTTTRRPVVKAKPIDPNRRVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDSIMACICATDFCNGYDGGSPVNLKXTTTPTPLRLPQSQTERPRILCHQCGSLFSGSGNPPCEEGNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANGERSVESDSSSSSNDRFDSSQVNQEQNNNRNRNDNNSKNNQQTSRRPTTTRRPSFTQTTPSSQFRXPSTPPPRSQNTFSGGPGLQCYSCGSFPLLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCESENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGFGNTILXPDFGWLDRPVPSVEENCLTWSITLARKGSRRRSSEGWHSDSCCNAIKYICELK 1338          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000005417 (pep:novel supercontig:LSalAtl2s:LSalAtl2s2921:9665:13131:1 gene:EMLSAG00000005417 transcript:EMLSAT00000005417 description:"maker-LSalAtl2s2921-snap-gene-0.4")

HSP 1 Score: 98.5969 bits (244), Expect = 5.132e-21
Identity = 83/289 (28.72%), Postives = 123/289 (42.56%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTT-----STTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRS--SIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            ++D  WSS+  W +CS SCG G + R R    PS  +GG SC G++++++ CN    CP++  WS + QW+SC+ TCG GR+  +RN      G GRPC G     R                 H  S   +   +      I     + TTL P+      +  T                        TT + T N          ++ Q        ++N+ R+  +IK   D  WS + +WS C+K CG G + R+R+   P++NGG  C G A     CN   C
Sbjct:  369 AIDGKWSSWQSWGQCSASCGNGHRXRARECNSPSPAHGGLSCFGNSVDNQVCNV-RECPIDASWSLWTQWTSCTKTCGEGRRERQRNCDGALFG-GRPCTGNSTVERD-----------MDIGLHGPSGQHV---VLHVVLEIDRGEEIVTTLLPNLEAEDVLELTLKMDHAVQIKIAQLMGXWNAWNQWTTCSATCNGGTQTRGRTCIEPQFGGKSCSGSSNDDRACGAIKCPIDASWSIWTSWSTCTKTCGGGSRRRSRTCT-PAQNGGQPCIGTATDESTCNTQEC 640          

HSP 2 Score: 76.6406 bits (187), Expect = 3.325e-14
Identity = 43/116 (37.07%), Postives = 66/116 (56.90%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE 356
            W+++ QW+ CS +C  GTQ R R  ++P   GGKSC G + + R C A   CP++  WS +  WS+C+ TCGGG +   R      +G G+PC+G   +  +C+T  C   G+  +
Sbjct:  536 WNAWNQWTTCSATCNGGTQTRGRTCIEPQF-GGKSCSGSSNDDRACGA-IKCPIDASWSIWTSWSTCTKTCGGGSRRRSRTCTPAQNG-GQPCIGTATDESTCNTQECIVIGSWAQ 648          

HSP 3 Score: 64.6994 bits (156), Expect = 1.494e-10
Identity = 44/116 (37.93%), Postives = 65/116 (56.03%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN----IRRRASGSGRPCLGEKAETRSCSTNPCSRFG 352
            WS++G+WS CS +CG GTQ R R     +   G +CRG + ESR+C+  + C ++  W+ + +W+ CSA+CG G +T  R+    I R     G+PC G   E   C T  C+  G
Sbjct:  262 WSAWGRWSTCSRTCGDGTQIRARTCADHNGVSG-NCRGSSTESRRCSI-SNCKIDGHWAEWTRWAPCSASCGTGSKTRFRSCSNPIPRYG---GKPCAGLDRENLKCFTKHCAIDG 372          

HSP 4 Score: 60.8474 bits (146), Expect = 2.308e-9
Identity = 44/116 (37.93%), Postives = 60/116 (51.72%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPV-NCEWSNFGQWSSCSATCGGG-RQTXRRNIRRRASGSGRPCLGEKAETRSC-STNPC 348
            VD +W  +  W  CSVSCG G Q R+R    P  ++GG++CRG ++ES  C   +   V +  W  + QWS CSATCG   R   R+         G  C G+  +  SC +T PC
Sbjct:  692 VDGMWGQWQFWGSCSVSCGNGVQSRRRQCDSPRPSSGGRNCRGSSVESNTCIKPSCIQVIDGNWGQWIQWSGCSATCGHATRSRSRQCDSPSPMNGGSFCSGDNNQISSCPNTRPC 807          

HSP 5 Score: 60.077 bits (144), Expect = 3.910e-9
Identity = 45/132 (34.09%), Postives = 67/132 (50.76%), Query Frame = 0

Query:  227 SASTSDD--TGSVDC----LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFG 352
            S S++DD   G++ C     WS +  WS C+ +CG G+++R R    P+ NGG+ C G A +   CN    C V   W+ +G+WS+CS +C GG ++  R        S   C G  +E   C+  PC   G
Sbjct:  571 SGSSNDDRACGAIKCPIDASWSIWTSWSTCTKTCGGGSRRRSRT-CTPAQNGGQPCIGTATDESTCNT-QECIVIGSWAQWGRWSTCSKSCDGGSRSRSRPC------SNNNCXGNGSENGRCNAKPCGVDG 694          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000002328 (pep:novel supercontig:LSalAtl2s:LSalAtl2s143:679982:683460:1 gene:EMLSAG00000002328 transcript:EMLSAT00000002328 description:"augustus_masked-LSalAtl2s143-processed-gene-6.1")

HSP 1 Score: 97.4413 bits (241), Expect = 1.408e-20
Identity = 84/283 (29.68%), Postives = 127/283 (44.88%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA-KTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVH-PSRNGGNECPGRAFQSRICNRHTCGSGA 520
            WS +  WS CS +C +G++ R+R    P+   GGK+CRG AIE+  CNA   CP+N +W+++G+WS CS +CGGG Q  RR       G G PC G+  + R C+  PC   G     S+ SS +   +            S  L T L   S ++  I +     S TT T    T ++ ++ + +    +       ++ +  P      T  S        D  W  +  W+ CS  CG G + R R   + P  +GG  C G   +   C +  C +G 
Sbjct:  489 WSEWDTWSPCSKTCESGSRFRQRHCNNPAPKFGGKTCRGSAIENGVCNADVNCPINGKWNSWGRWSICSVSCGGGIQGRRRTCTVPQFG-GEPCNGQSDQERQCNQIPCPVDGVWSLWSKWSSCSKTCEGGFRQRIREC-ISPQLGTEL--CSGSSREIESCNNKISCTTDTSNWNTWSNWSSCSASCGHGL------RVRSRACP------TKGSCEVGLEFSDGNWGYWQEWTSCSVSCGKGQKTRGRFCNNPPPSDGGKPCSGYGLEKSYCIQEECTTGG 755          

HSP 2 Score: 75.8702 bits (185), Expect = 5.334e-14
Identity = 90/326 (27.61%), Postives = 128/326 (39.26%), Query Frame = 0

Query:  212 GVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPC----------------SRFGAKTESRHSSSNSDI--DNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTT-TTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            GV   CVG   KS  + + S     +D  WS + +W+ CS +CGTGT+ R R    P    GGK C G A ES +C +   CPV   WS++  W  CS TCG G +   R     +   G P C G   E+  C    C                   G  T+ R  + +       S S +F+      P +  +           T    +   T +P S T  S +       N P         +   I +   +   N   + K      W+ +  WS+CS  CG G+Q R R+   P + GG  C G++ Q R CN+  C
Sbjct:  290 GVSGNCVG---KSDETRTCSVLACKIDGGWSIWTKWTPCSSTCGTGTKARFRSCSNPIPKYGGKICVGXASESAKCFS-RYCPVEGRWSSWKSWGGCSVTCGEGTRHRNRYCDSPSPAHGGPSCFGPPVESTKCIEKECPIDGIWLSWTQWTTCSKTCGPGTQERTRTCDGPQFGGQSCSGNFTIERDVIPCWQIVV--------CPTDGEWSEWDTWSPCSKTCESGSRFRQRHCNNPAPKFGGKTCRGSAIENGVCNADVNCPINGK------WNSWGRWSICSVSCGGGIQGRRRTCTVP-QFGGEPCNGQSDQERQCNQIPC 596          

HSP 3 Score: 64.6994 bits (156), Expect = 1.421e-10
Identity = 78/288 (27.08%), Postives = 122/288 (42.36%), Query Frame = 0

Query:  253 SCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFST------IPTSSPLY---TTLFPSSTTTTTI-----------TTTTTTTSTTTTTPTSTTTTSTT------TTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICN 512
            +CG GTQ+R R  L  +   G +C G + E+R C+  A C ++  WS + +W+ CS+TCG G +   R+         G+ C+G  +E+  C +  C       E R SS  S    S++    T        + SP +   +   P   +T  I           + T  TT + T  P +   T T         + + N  I+ +V    +    P D                  EWS++DTWS CSK C  G ++R R   +P+ + GG  C G A ++ +CN
Sbjct:  273 TCGDGTQKRDRTCLDRNGVSG-NCVGKSDETRTCSVLA-CKIDGGWSIWTKWTPCSSTCGTGTKARFRSCSNPIPKYGGKICVGXASESAKCFSRYC-----PVEGRWSSWKSWGGCSVTCGEGTRHRNRYCDSPSPAHGGPSCFGPPVESTKCIEKECPIDGIWLSWTQWTTCSKTCGPGTQERTRTCDGPQFGGQSCSGNFTIERDVIPCWQIVVCPTDG-----------------EWSEWDTWSPCSKTCESGSRFRQRHCNNPAPKFGGKTCRGSAIENGVCN 536          

HSP 4 Score: 57.3806 bits (137), Expect = 2.793e-8
Identity = 44/139 (31.65%), Postives = 60/139 (43.17%), Query Frame = 0

Query:  211 EGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQ-PSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
             G+RV     S+  P+  S        D  W  + +W+ CSVSCG G + R R     P ++GGK C G  +E   C     C     W  +G WS+C+ TCG   +T  R     +      C GE  +T  CS   C
Sbjct:  674 HGLRVR----SRACPTKGSCEVGLEFSDGNWGYWQEWTSCSVSCGKGQKTRGRFCNNPPPSDGGKPCSGYGLEKSYC-IQEECTTGGSWGQWGTWSACTLTCGNSVRTRARTCHNSS------CEGEGIQTERCSVPKC 801          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000001943 (pep:novel supercontig:LSalAtl2s:LSalAtl2s133:467280:542329:-1 gene:EMLSAG00000001943 transcript:EMLSAT00000001943 description:"maker-LSalAtl2s133-augustus-gene-4.14")

HSP 1 Score: 84.7297 bits (208), Expect = 1.716e-16
Identity = 72/290 (24.83%), Postives = 121/290 (41.72%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN--IRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNS---------DIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPI--PDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            ++  + +WS CS +CG G Q+RKR         G  C G +IE+++C +G  CPV  EWS+   W+ CS TCGGG++   R   +       G  C GE   ++ C+  PC   G  ++    SS+          +    I+   +  P   PL+     +     + +  +  +  + +        S     ++       E     +  P   P D +   N  R          WS +  W+ CS+ C  G Q R R  ++  R+ G +C G +++ + CN+ TC
Sbjct:  696 VYGPWSEWSACSTTCGEGIQKRKREC------SGDFCEGASIETKKC-SGNVCPVWTEWSD---WTECSVTCGGGKKXKVRECVLPSGERSRGLICPGEGYISKKCNNYPCPELGPWSDWSSCSSSCGSGRRTRIRECGLPITRGSNNNPCLKPLFEIEVCNPQKCPSFSPWSEWSECSVSCGGGYRKKSRECKASS------RESRQVFRGNPCHGPLDFVEECNKQRCPF-------WSSWGEWTSCSQSCNGGRQKRYRECLNDDRSLGEDCNGDSYEEKACNKETC 962          

HSP 2 Score: 68.5514 bits (166), Expect = 1.357e-11
Identity = 39/91 (42.86%), Postives = 54/91 (59.34%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGS 329
             WSS+G+W+ CS SC  G Q+R R  L    + G+ C GD+ E + CN   TCP+  +WS+   W+SCS +CG GG +T  R  +R A  S
Sbjct:  911 FWSSWGEWTSCSQSCNGGRQKRYRECLNDDRSLGEDCNGDSYEEKACNK-ETCPIYNKWSD---WTSCSVSCGSGGXRTKIRTCKRDAPQS 997          

HSP 3 Score: 67.0106 bits (162), Expect = 4.163e-11
Identity = 46/114 (40.35%), Postives = 56/114 (49.12%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGK------SCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            W  +GQWS CS SCG G+Q RKR  L P    G        C G +  +  CN    CP    WS +  WSSCS+TCGGG +      +R    SG  C G + ET  C+  PC
Sbjct: 1424 WXEWGQWSSCSASCGGGSQLRKRECLSPGGRYGPFLESEGPCPGPSEITLFCNLD-DCPT--VWSEWTPWSSCSSTCGGGSK------KRTRVCSGMQCEGNQLETSLCNDRPC 1528          

HSP 4 Score: 64.3142 bits (155), Expect = 2.342e-10
Identity = 72/283 (25.44%), Postives = 104/283 (36.75%), Query Frame = 0

Query:  244 YGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSS-------SNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDC------EWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNR 513
            + +WS CS +CG GT++R R  +      G  C  D  E+  CN    CPV   WS + +W+ CS +CG G ++  +      S     C G+      C+ NPC      TE    S          + D  +S S    P   PL      +S +    +  T  T  + +    T T      +                         S  N+   S K  ++C       W+ +  WSVCS  CG G + RNR     +RN    C G + Q   C R
Sbjct: 1141 WSEWSTCSKTCGKGTKKRNRKCIDSKFRNGNPCNADLNETENCNE-QDCPV---WSPWTEWTECSRSCGKGMRSKMKICLDLTS-----CSGKTKYMELCNENPCPSLTPWTEWTPCSLSCGGGXQRRERDCLVSRSGFLNPCFEPLEEIQRCNSESCPKWSEWTEWTPCSESCGGGTQTRVRDCVSR------------------------SACNSLGDSDKEKRECSVNACPSWTSWSNWSVCSASCGGGTRIRNRECXLNARNKA-PCIGDSVQREECGR 1389          

HSP 5 Score: 60.8474 bits (146), Expect = 2.859e-9
Identity = 35/87 (40.23%), Postives = 48/87 (55.17%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKS---CRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIR 323
            W+ + +WS CSV+CG G Q+R R  L P    G +   C GD  E + CN    CP   +W+ +  WS CS TCG GGR+  +R  +
Sbjct: 1016 WTQWTEWSTCSVTCGGGNQKRIRDCLTPRNLNGSNIYGCDGDTWEMKACNV-RKCP---QWAEWSSWSECSVTCGSGGRRNRKRECK 1098          

HSP 6 Score: 59.6918 bits (143), Expect = 6.809e-9
Identity = 69/273 (25.27%), Postives = 96/273 (35.16%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMIL--------------------------QPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAAL-KDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYR 486
            W+S+  WS CS SCG GT+ R R                             + S++  K C G  + S  CN    CP    W  +GQWSSCSA+CGGG Q  +R            CL           +P  R+G   ES                    P   P   TLF +     T+ +  T  S+ ++T    +   T   +        N++  +L  D+P                       WS + +W+VCS  CG G Q R
Sbjct: 1343 WTSWSNWSVCSASCGGGTRIRNRECXLNARNKAPCIGDSVQREECGRRNRKRKCSSDQSKDCTGSGLISEPCNENKDCPSG--WXEWGQWSSCSASCGGGSQLRKRE-----------CL-----------SPGGRYGPFLESEG------------------PCPGPSEITLFCNLDDCPTVWSEWTPWSSCSSTCGGGSKKRTRVCSGMQCE--GNQLETSLCNDRPCYS--------------------WSHWGSWTVCSVSCGQGTQKR 1551          

HSP 7 Score: 58.151 bits (139), Expect = 1.968e-8
Identity = 78/311 (25.08%), Postives = 114/311 (36.66%), Query Frame = 0

Query:  242 SSYGQWSRCSVSCGTGTQQRKRMI---LQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRR---------NIRRRASGSGRPCLGEKAETRSCSTNPCSRF------------------------GAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            S++ +W+ C+  CGTG + R+R     + PST     C+    E   CN    CP   EWS+   W+SCS +CGGG Q  RR         NI  +  G      G   E R C++N C  +                        G     R   S+   D    + F     +S     L P S  T   TT  +T     T      +      T        N   A LK+       + + NNS+  +       +  +  WS CS  CG G+Q R R         G+ C G + +++ C+ + C
Sbjct:  471 SNWSEWTICTKKCGTGERSRERTCNGEIYPSTQ--NPCQISLYERELCNT-QPCPSYTEWSD---WASCSVSCGGGIQKRRRSCVLPKFSSNIDLKCEG------GPPVEKRDCNSNACPNWSSWGSWSSCSLSCGEGKRRKTRVCGYFDAERMKISDKVSDLCPGNGFKEEKCNSNRCPLLGPWSEWTECSTTCGSTGIRQRTRECGINSGLLRGVT--------NACQARLKE-------IESCNNSKCPV-------YGPWSEWSACSTTCGEGIQKRKREC------SGDFCEGASIETKKCSGNVC 741          

HSP 8 Score: 57.3806 bits (137), Expect = 2.899e-8
Identity = 38/120 (31.67%), Postives = 57/120 (47.50%), Query Frame = 0

Query:  238 DC-LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE 356
            DC +WS + +W+ CS SCG G + + ++ L  +     SC G       CN    CP    W+   +W+ CS +CGGG Q   R+     SG   PC     E + C++  C ++   TE
Sbjct: 1187 DCPVWSPWTEWTECSRSCGKGMRSKMKICLDLT-----SCSGKTKYMELCNENP-CPSLTPWT---EWTPCSLSCGGGXQRRERDCLVSRSGFLNPCFEPLEEIQRCNSESCPKWSEWTE 1297          

HSP 9 Score: 56.225 bits (134), Expect = 6.346e-8
Identity = 37/103 (35.92%), Postives = 52/103 (50.49%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSC 343
            WS + +W+ CS SCG GTQ R R  +  S +   S      E R+C+  A CP    W+++  WS CSA+CGGG +   R     A     PC+G+  +   C
Sbjct: 1292 WSEWTEWTPCSESCGGGTQTRVRDCV--SRSACNSLGDSDKEKRECSVNA-CP---SWTSWSNWSVCSASCGGGTRIRNRECXLNARNKA-PCIGDSVQREEC 1387          

HSP 10 Score: 56.225 bits (134), Expect = 6.904e-8
Identity = 111/490 (22.65%), Postives = 179/490 (36.53%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDE-------PWEGKWEEGVRV--ECVGGSKKSPSSASTSDDTGSVDC----------------LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIR--RRASGSG-RPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDID---------NSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCE-WSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGS 518
            WSD+SS S    S     + RE  + I      +PC     E  VC+ Q+CP F        C +         +C G Y+           R     +R++R +F                  G+  H   LD  E       P+   W E       C GG +K        D +   DC                +++ +  W+ CSVSCG+G  + K  I     +  +SC+ D I+   CN    CP    W+ + +WS+CS TCGGG Q   R+    R  +GS    C G+  E ++C+   C ++   +     S                    SF+     + +   LF     + T +      +       S  +T + T    T       +++  ++    + DL+ T N        QDC  WS +  W+ CS+ CG G++ + +  +  +      C G+     +CN + C S
Sbjct:  802 WSDWSSCSSSCGS-GRRTRIRECGLPITRGSNNNPCLKPLFEIEVCNPQKCPSFSPWSEWSECSV---------SCGGGYR--------KKSRECKASSRESRQVF-----------------RGNPCH-GPLDFVEECNKQRCPFWSSWGEWTSCSQSCNGGRQKRYRECLNDDRSLGEDCNGDSYEEKACNKETCPIYNKWSDWTSCSVSCGSGGXRTK--IRTCKRDAPQSCK-DEIKKEMCNEDQPCP---SWTQWTEWSTCSVTCGGGNQKRIRDCLTPRNLNGSNIYGCDGDTWEMKACNVRKCPQWAEWSSWSECSVTCGSGGRRNRKRECKEFQFSFNEDVVVN-MEKDLFCPGRYSQTQSCNNGIKNCPFPGEWSEWSTCSKTCGKGTKKRNRKCIDSKFRNGNPCNADLNETENCNE-----QDCPVWSPWTEWTECSRSCGKGMRSKMKICLDLT-----SCSGKTKYMELCNENPCPS 1238          

HSP 11 Score: 52.373 bits (124), Expect = 9.476e-7
Identity = 39/115 (33.91%), Postives = 52/115 (45.22%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPST---NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGR-PCLGEKAETRSCSTNPCSRF 351
            WS + QWS CS SCG G Q R R     S+       +C G   E + CN    CP   E SN+ +W+ C+  CG G ++  R        S + PC     E   C+T PC  +
Sbjct:  414 WSFWSQWSSCSASCGGGKQSRSRTCQVSSSIFLGVNVTCPGSPEEFQNCNTNK-CP---ELSNWSEWTICTKKCGTGERSRERTCNGEIYPSTQNPCQISLYERELCNTQPCPSY 524          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000002798 (pep:novel supercontig:LSalAtl2s:LSalAtl2s16161:6:765:1 gene:EMLSAG00000002798 transcript:EMLSAT00000002798 description:"augustus_masked-LSalAtl2s16161-processed-gene-0.0")

HSP 1 Score: 77.0258 bits (188), Expect = 1.704e-15
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETR 341
            VD  WSS+  W +CS SCG G +QR R    PS  +GG SC G++++ + CN    CP++  WS + QW+SC+ TCG GR+  +RN      G GRPC G     R
Sbjct:   13 VDGKWSSWQSWGQCSASCGNGRRQRARECNSPSPAHGGLSCFGNSVDXQVCNV-RECPIDASWSLWTQWTSCTKTCGEGRRERQRNCDGALFG-GRPCTGNSTVER 116          

HSP 2 Score: 62.003 bits (149), Expect = 1.149e-10
Identity = 40/114 (35.09%), Postives = 59/114 (51.75%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQ---CNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTN 346
            +D  WS + QW+ C+ +CG G ++R+R     +  GG+ C G++   R    C     C ++  WS++ QWS+CS TCG G +  RR+    A    GR C G   E   CS N
Sbjct:   70 IDASWSLWTQWTSCTKTCGEGRRERQRNC-DGALFGGRPCTGNSTVERDVIPCWIQVVCSLDGHWSSWAQWSTCSTTCGAGNRQRRRDCNNPAPKFGGRRCAGADFENGPCSAN 182          

HSP 3 Score: 50.447 bits (119), Expect = 6.615e-7
Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPV 294
            S+D  WSS+ QWS CS +CG G +QR+R    P+   GG+ C G   E+  C+A   CP+
Sbjct:  128 SLDGHWSSWAQWSTCSTTCGAGNRQRRRDCNNPAPKFGGRRCAGADFENGPCSANQNCPI 187          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000009829 (pep:novel supercontig:LSalAtl2s:LSalAtl2s63:1003125:1030351:1 gene:EMLSAG00000009829 transcript:EMLSAT00000009829 description:"maker-LSalAtl2s63-augustus-gene-10.41")

HSP 1 Score: 78.1814 bits (191), Expect = 1.508e-14
Identity = 47/142 (33.10%), Postives = 73/142 (51.41%), Query Frame = 0

Query:  208 KWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGT-GTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATC---GGGRQTXRRNIRRRASGSGRPC-LGEKAETRSC 343
            K + G R+ C+GGS            T  +D  W+ +  W  C++ CG  G + R+R   +P  ++GGK C GD  ++ +C     CP++C WSN+G W+ CS TC   G G +  RR++   A+  G  C  G   E+ +C
Sbjct: 2003 KRQLGTRMHCLGGSHFIDERCFPQKCT--IDGGWTDWLNWEDCNILCGKKGKRIRRRYCAKPIPSHGGKDCYGDKKQTGKCPKLEPCPIHCIWSNWGHWTKCSVTCGPIGYGYRERRRHVAVXANYGGSNCDKGAYLESENC 2142          

HSP 2 Score: 58.151 bits (139), Expect = 1.995e-8
Identity = 37/138 (26.81%), Postives = 63/138 (45.65%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCG---TGTQQRKRMILQPSTNGGKSC-RGDAIESRQC---------NAGATCPVNCEWSNFGQWSSCSATCGGGR---------QTXRRNIRRRASGSGRPC-----LGEKAETRSCSTNP 347
            + C+WS++G W++CSV+CG    G ++R+R +   +  GG +C +G  +ES  C              CP++C+WS +     C +   G               R +++RA  +G+ C       EK+    CS  P
Sbjct: 2089 IHCIWSNWGHWTKCSVTCGPIGYGYRERRRHVAVXANYGGSNCDKGAYLESENCPHCEMEKSKQCIPYCPIDCDWSPWNFMGECLSCYHGDSLPPNSKNYPAKKIRFVKKRAKYNGKECETKGATQEKSVDLDCSQVP 2226          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000001236 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1201:44563:70317:1 gene:EMLSAG00000001236 transcript:EMLSAT00000001236 description:"maker-LSalAtl2s1201-snap-gene-0.14")

HSP 1 Score: 73.1738 bits (178), Expect = 3.528e-13
Identity = 41/113 (36.28%), Postives = 60/113 (53.10%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQ-RKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQT----XRRNIRRRASGSGRPCLGEKAETRSC 343
            VD  WS++  W  C+ +CG G ++ ++R  + P   NGGK C G+ +E   C     CPV+C +S +G WS CS +CG G         R+I + A   G  C G K E ++C
Sbjct:  553 VDGGWSTWQNWGECNRACGEGGKRPKRRXCIHPFPENGGKDCSGNGVEYGPCEVLKPCPVHCLYSEWGVWSPCSVSCGNGPGVGTVERNRHIYQEAKYGGIKCGGIKREIKNC 665          

HSP 2 Score: 55.4546 bits (132), Expect = 9.682e-8
Identity = 90/405 (22.22%), Postives = 145/405 (35.80%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSC-NG---GVQFRERKILILARQGGSPCRGERKESRVC------STQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVT--NNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCL------WSSYGQWSRCSVSCG-TGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNC---EWSNFGQWSSCSATCGGGRQTXRR-----NIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD 440
            + C +S++  WS CS SC NG   G   R R I   A+ GG  C G ++E + C         Q       +P   CP ++ +   +    G+  +   N    +       +  +K R+++   G    + GK   L  G +  +            +EE V    V    + P      D       +      + S+  WS C+  CG  G Q+R R+ +     GG S   ++    +      CP  C   EW+ +  WS C  TCG G+   RR     N  ++       C+G + E + C    C        S+H     DID     S + I    P    L  ++  T  + +T     +             T    T N  + +E +  +KD
Sbjct:  612 VHCLYSEWGVWSPCSVSCGNGPGVGTVERNRHIYQEAKYGGIKCGGIKREIKNCIHCNYTKNIQMEDIQNCIP--YCP-IDCVWEEWGQITGKCVICWGNQAGQFPKAVAKLRSRKKIRFVYIPPG----NGGKECILSDGQNRQKDQF---------YEEAVLPCKVSEEIERPQKNEHFDTYKCKSTMVQGKPFFESWTMWSGCTAVCGRDGIQKRTRVCV-----GGSSKECESYTKFKSEETMPCPSVCDPPEWTMWENWSKCEVTCGIGQVARRRTCMAXNRDKKFRKEAITCIGIQREQKKCDMGACG-------SKH-----DIDYEGEDS-TGISGQWPSDYKLVEANHETVNLNSTKQDEDSLLVQNLEQQQPYDTKQHQTNNRIVKSEPDVVIKD 982          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000011499 (pep:novel supercontig:LSalAtl2s:LSalAtl2s796:74576:77471:1 gene:EMLSAG00000011499 transcript:EMLSAT00000011499 description:"augustus_masked-LSalAtl2s796-processed-gene-0.0")

HSP 1 Score: 68.5514 bits (166), Expect = 9.118e-12
Identity = 47/154 (30.52%), Postives = 65/154 (42.21%), Query Frame = 0

Query:  220 GSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCG------------------TGTQQR-------------KRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETR 341
            G+K S    S      S+ C WS +  WS CSVSCG                  +GT+               K+ +L P   GGK C GD +E+++C +GA CPV+ +WS +     C + CG G +T  R          GRPC    +  R
Sbjct:  154 GTKDSSPKFSAIIQNSSISCRWSKWSSWSHCSVSCGNSPGRRXRSRSQNGSRLCSGTENESKKCIVNRCAQDCKKKVLLPHLYGGKPCIGDDLETKRC-SGADCPVDGDWSQWSHXGXCQSKCGVGNRTRTRACDNPPPENGGRPCEENNSNVR 306          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000010397 (pep:novel supercontig:LSalAtl2s:LSalAtl2s68:326217:339785:1 gene:EMLSAG00000010397 transcript:EMLSAT00000010397 description:"maker-LSalAtl2s68-snap-gene-3.35")

HSP 1 Score: 68.1662 bits (165), Expect = 9.435e-12
Identity = 49/132 (37.12%), Postives = 71/132 (53.79%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQ---CNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNS 364
            VD  WS + +W+ CS SC  G + R R   +P   GG+SC G+    +    C     C V+ +WSN+ +WSSC+ TCGGG +  RRN    A    G+ CLG + +T++C T+ C   G  ++ R  S  S
Sbjct:  399 VDAEWSLWSRWTACSKSCDRGQKSRMRHCGEP-LFGGQSCPGNITIEKDVIDCWVRQVCTVDGKWSNWLEWSSCTVTCGGGTRQRRRNCDNPAPKFGGQRCLGSEFDTQNCETDSCPIDGRWSQWRQWSQCS 529          

HSP 2 Score: 67.781 bits (164), Expect = 1.309e-11
Identity = 77/276 (27.90%), Postives = 107/276 (38.77%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC---------SRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNEC 501
            +D  W+ + +WS CS SCGTG + R R    P    GG  C G   E+ +C +   CP+   WS++ +W   S +CG G RQ  RR      +  G  C+GE ++T +     C         SR+ A ++S      S + +     F     S P   T+             T     +     S+ T +    T       DN        Q     +  T N    S     D  WS +  WS CSK CG G Q R R   +P R GG  C
Sbjct:  285 IDGEWARWTRWSPCSTSCGTGFRSRSRACSDPIPRYGGNRCSGSPTETVKCFS-TFCPIAGRWSSWQZWGQLSVSCGEGTRQRQRRCDSPAPAHRGASCIGESSQTNTLFIKECPVDAEWSLWSRWTACSKSCDRGQKSRMRHCGEPLFGG--QSCPGNITIEKDVIDCWVRQVCTVDGKWSNWLEWSSCTVTCGGGTRQRRRNCDNPA-PKFGGQRCLGSEFDTQNCETDSCP--IDGRWSQWRQWSQCSKTCGSGSQKRERVCNNP-RFGGKAC 553          

HSP 3 Score: 65.0846 bits (157), Expect = 8.319e-11
Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRR 320
            +VD  WS++ +WS C+V+CG GT+QR+R    P+   GG+ C G   +++ C    +CP++  WS + QWS CS TCG G Q   R
Sbjct:  457 TVDGKWSNWLEWSSCTVTCGGGTRQRRRNCDNPAPKFGGQRCLGSEFDTQNCET-DSCPIDGRWSQWRQWSQCSKTCGSGSQKRER 541          

BLAST of EMLSAG00000000316 vs. L. salmonis peptides
Match: EMLSAP00000007552 (pep:novel supercontig:LSalAtl2s:LSalAtl2s436:271825:290086:-1 gene:EMLSAG00000007552 transcript:EMLSAT00000007552 description:"maker-LSalAtl2s436-snap-gene-3.19")

HSP 1 Score: 66.2402 bits (160), Expect = 4.145e-11
Identity = 43/121 (35.54%), Postives = 62/121 (51.24%), Query Frame = 0

Query:  228 ASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN---IRRRASGSGRPCLGEKAETRSCS 344
            +S ++ + SV   W  + QW+ C+VSCG G + R R+   PS  NGG  C+G A++S  C+ G     +  +S + QWS CS  C G R    RN      +    GR C G + E R C+
Sbjct:  468 SSCNEASCSVHGNWGKWTQWTYCTVSCGRGRKSRSRLCNNPSLQNGGNHCQGRALDSTFCSNGLCSIADYGFSEWTQWSKCSVDCIGDRGNNVRNRFCSTSKLQFGGRNCKGSRKEKRLCT 588          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|110287971|sp|Q2PC93.1|SSPO_CHICK (RecName: Full=SCO-spondin; Flags: Precursor)

HSP 1 Score: 101.293 bits (251), Expect = 4.551e-20
Identity = 81/290 (27.93%), Postives = 128/290 (44.14%), Query Frame = 0

Query:  228 ASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGG-RQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD---QPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHP-SRNGGNECPGRAFQSRICN 512
            A  SD++   DC+ S +G WS+CS SCG G   R+R++L+ +  GG +C    +++R C   A CPV   W+ +G WSSC A CGGG R   R          G+PC GE  +++ C+  PC       ++R       +          +P    +   L  +S+  +                 +    S           + ++V   L+D   Q +  D + T  ++   I     C WS +  W++C + CGVG+Q R RS  +P + NGG  C G   + R C+
Sbjct: 2565 ADGSDESSCADCILSPWGGWSQCSHSCGLGVTSRQRVLLRGALPGG-TCHTPRLDTRACFLRA-CPVPGAWAAWGVWSSCDAECGGGMRSRTRSCTDPPPKNGGQPCAGEALQSQPCNLQPCG------DTRECGPGMVLVQEGDCVQGLVPPCPQVCGDLSATSSCQSPCQEGCRCPPGLFLQEGTCVNASQCHCHQGQQRWLPSQV--FLRDGCSQCVCRDGVVTCEDTACPIA----CAWSAWSLWTLCDRSCGVGMQERFRSPSNPAAANGGAPCDGDTREVRECH 2840          

HSP 2 Score: 83.5741 bits (205), Expect = 1.000e-14
Identity = 115/480 (23.96%), Postives = 164/480 (34.17%), Query Frame = 0

Query:   64 DCKWSDFSSWSDCSQSCNGGVQFRERKIL-ILARQGGSPCRGERKESRVC--STQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE--SRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNA----------ALKDQPIPDDDLSTTNNSRSSIKRVQDCE---------WSDYDTWSVCSKPC-GVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHTC 516
            D  W+ +  WS C     GG   R R      AR GG PC GE ++SR C  +T  CP   G +    C             D     T   +P A   P  +  +    +        W+  K      GS W + G    +  E +  E V+  C   + KS +    ++     D  WS +G WS CS  C  GTQ   R    P+   GG+ C G +   R C A   CP    W  +  W  CSA+CGGG Q   R+           C G   ++++C+T+ C   G       R          S +     I          FP          T T               +        N+  D +             AL  +  P   + +   S + +    +C          +S +  WS CS+ C G+GV  R R   +P    GG +C G    S+ C    C
Sbjct: 3477 DGAWAAWGPWSGCGGC--GGQAVRTRSCSSPPARFGGLPCAGEARQSRACPWATSSCPECAGGLVAFTCGKPCPHSCEDLREDTACMATPRCLP-ACACPHGQLLQDGDCVPPELCRCAWAPSK-----NGSIWEQDGAVPMQ--ELQPGETVQRHCQNCTCKSGTLQCHAEPGCRADGGWSPWGPWSPCSPGCQAGTQLASRQCNNPTPQLGGRGCSGHSQRQRPCPATEGCPEEEPWGEWSPWGPCSASCGGGEQLRHRDCPPPGG-----CPGLALQSKTCNTHVCREAGCPPGRLYRECQQGEGCPYSCAHLAGRI--------ACFPGGCQEGCHCPTGTLLHHGHCLQECPCVLTAEVLRKLRNSSADLQAAPHLLGTRGPPLALDQELPPGSTIHSACTSCTCLHGRLNCSEPVCPRDGGFSPWGPWSSCSRSCGGLGVMTRRRGCTNPEPARGGRDCAGPRSDSKYCQSPEC 3933          

HSP 3 Score: 80.8777 bits (198), Expect = 7.415e-14
Identity = 76/289 (26.30%), Postives = 106/289 (36.68%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCS-------RFGAKTESRHSSSN-----SDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNR-SIVHPSRNGGNECPGRAFQSRICNRHTC 516
            WSS+  WS CS SC     QR R        G  +C G  ++   C+  A CPV   W  +  WS CSA C  G QT  R     A G G  C G   +TR+C+T PC        ++    E RHS         D+   ++ +    P        L   + T    +            P       T    T     +  ++  + +  P+P                   C WS++  WS CS  C VG++ R R  IV P   GG  C G + +   C+   C
Sbjct: 2852 WSSWTPWSPCSRSCFHHVDQRGRRHRFRHCEGMGTCPGLGVQEEPCDT-APCPVAGVWMPWSAWSECSAPCDAGVQTRSRTCTPPAFG-GAECTGPHLQTRNCNTRPCGAQCPDTMQYLTAEECRHSEGRCPWICQDLGAGVACTAQCQPGCH-CPAGLLLQNGTCVPPSHCLCHHRGHLYQPGDINALDTCNNCTC----VTGQMVCSTETCPVP-------------------CTWSNWTAWSTCSHSCDVGMRRRYRVPIVPPLAGGGPPCQGPSMEVEFCSLQPC 3114          

HSP 4 Score: 74.3294 bits (181), Expect = 7.979e-12
Identity = 87/313 (27.80%), Postives = 122/313 (38.98%), Query Frame = 0

Query:   59 FDGRIDCK---------WSDFSSWSDCSQSCNG-GVQFRERKIL-ILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSAST--------SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDA---IESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPC 348
              GR++C          +S +  WS CS+SC G GV  R R        +GG  C G R +S+ C + +CP     VP    P   V GA         +      PW+   P  K                          T +H  R    T E    +   G  V    G ++ P +            +D   ++C W+ +G W+ CS SCG G QQR R    P  + G+ C G     ++ R C+  A C V+  WS +  WS C  TCGGGR    R+  R      G+ C GE+ +   C+  PC
Sbjct: 3864 LHGRLNCSEPVCPRDGGFSPWGPWSSCSRSCGGLGVMTRRRGCTNPEPARGGRDCAGPRSDSKYCQSPECP----AVPTTE-PGPGVAGA---------EEEEGFGPWSPWSPCSK--------------------------TCTHPERPATKTRE----RPCVGTAVCSGDGFQEQPCNLPLCSDVPPCQGEDCAGLNCSWAPWGPWTECSRSCGVGRQQRLRAYSPPGAS-GRWCPGILSAFVQRRFCSLQA-CKVDGAWSAWSPWSRCDRTCGGGRAVRTRSCTRPPPKNGGQRCPGERHQLHLCNAQPC 4130          

HSP 5 Score: 61.2326 bits (147), Expect = 5.897e-8
Identity = 47/128 (36.72%), Postives = 62/128 (48.44%), Query Frame = 0

Query:  230 TSDDTGS-VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAG-ATCPVNCEWSNFGQWSSCSATC-GGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            T +DT   + C WS++  W+ C  SCG G Q+R R    P + NGG  C GD  E R+C+   AT   +  WS++  WS CS +C     Q  RR+  R   G G  C G   +   C T PC   G 
Sbjct: 2781 TCEDTACPIACAWSAWSLWTLCDRSCGVGMQERFRSPSNPAAANGGAPCDGDTREVRECHTPCATAEPSSGWSSWTPWSPCSRSCFHHVDQRGRRHRFRHCEGMGT-CPGLGVQEEPCDTAPCPVAGV 2907          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|341942022|sp|Q60519.2|SEM5B_MOUSE (RecName: Full=Semaphorin-5B; AltName: Full=Semaphorin-G; Short=Sema G; Flags: Precursor)

HSP 1 Score: 92.8189 bits (229), Expect = 1.225e-17
Identity = 77/279 (27.60%), Postives = 114/279 (40.86%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTT-TTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHTC 516
            W+++  W++CS SCG G Q R+R    P+  +GG+ C G + E R CN    CPV   W+++G WS CS  CGGG Q+     RRR+  +G  C G   E ++C+   C      T                            +T   P + T            T     P           T T   P D     A     + +D L + + S  ++    +  W+ +  WS CS+ C +G + R R+  +P  RNGG  C G A + + CN   C
Sbjct:  609 WTAWSSWAQCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQS-----RRRSCENGNSCPGCGVEFKTCNPEACPEVRRNTP---------------------------WTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADG--TGACDTDALVEDLLRSGSTSPHTL----NGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQAC 849          

HSP 2 Score: 90.8929 bits (224), Expect = 4.530e-17
Identity = 90/284 (31.69%), Postives = 117/284 (41.20%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPC 348
            W+ + SWS CS +C GGVQ R R       + G+ C G   E + C+ + CP    + P      VNV     A  + R++ T  + P      +    R+       A G G                    DTD       E+ +R            S STS  T  ++  W+++G WS CS  C  G + RKR    P   NGG  C GDA E + CN  A CPV   WS +  WS CSA+CGGG     R+    A   G   CLG   E   CST  C
Sbjct:  667 WASWGSWSKCSNNCGGGVQSRRRSC-----ENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGG-ARQEQRFRFT-CRAPLPDPHGLQFGKRRTETRTCPADGTG------------------ACDTD----ALVEDLLR------------SGSTSPHT--LNGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQA-CPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCSTQAC 906          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|212276522|sp|Q9P283.4|SEM5B_HUMAN (RecName: Full=Semaphorin-5B)

HSP 1 Score: 89.7373 bits (221), Expect = 9.906e-17
Identity = 95/308 (30.84%), Postives = 122/308 (39.61%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPCSRF-------------GAKTESRH 359
            W+ + SWS CS +C GG+Q R R     A + G+ C G   E + C+ + CP    + P      VNV     A  + R++ T  + P A    +    R+       A G G                    DTD   E     G                STS  T  V   W+++G WS CS  C  G + RKR    P   NGG  C GDA E + CN  A CPV   WS +  WS CSA+CGGG     R+    A   G   CLG   E   C+T  C                GA++ SRH
Sbjct:  725 WASWGSWSKCSSNCGGGMQSRRR-----ACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGG-ARQEQRFRFTC-RAPLADPHGLQFGRRRTETRTCPADGSG------------------SCDTDALVEVLLRSG----------------STSPHT--VSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQA-CPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRH 988          

HSP 2 Score: 84.7297 bits (208), Expect = 3.315e-15
Identity = 49/129 (37.98%), Postives = 67/129 (51.94%), Query Frame = 0

Query:  235 GSVDCL--------------WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            G +DCL              W+ +  W+ CS SCG G Q R+R    P+  +GG+ C G + E R CN    CPV   W+++G WS CS+ CGGG Q+     RRRA  +G  CLG   E ++C+   C
Sbjct:  647 GGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQS-----RRRACENGNSCLGCGVEFKTCNPEGC 770          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|109939725|sp|Q13591.3|SEM5A_HUMAN (RecName: Full=Semaphorin-5A; AltName: Full=Semaphorin-F; Short=Sema F; Flags: Precursor)

HSP 1 Score: 87.8113 bits (216), Expect = 4.254e-16
Identity = 91/320 (28.44%), Postives = 129/320 (40.31%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKIL-ILARQGGSPCRGERKESRVCSTQQC--PHFV--GDVPDVNCPL-----------VNVIGATYANCDGRYKVTNNK--------VPWASERPV------------YKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPC 348
            W+ ++SWS CS +C  G Q R+R       R GG  C G+ +E R C+      PH    G  P   C             +   G   A C+  Y+  N           PW    PV            +++T K R    N   +G    +R  ++  S    SG  TD         G+  + +   + S           +V+  WS++  WS+CS  C  G + RKR+   P    GG  C G ++E ++CN    CPV+  WS +  W+ CSATCGGG     R+    A    G  CLG   E   C+T PC
Sbjct:  598 WTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVG---RQRIEMRYCSSDGTSGCSTD---------GLSGDFLRAGRYS---------AHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEYQECNI-LPCPVDGVWSCWSPWTKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQPC 895          

HSP 2 Score: 84.7297 bits (208), Expect = 3.346e-15
Identity = 46/116 (39.66%), Postives = 60/116 (51.72%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKT 355
            W+ +  WS CS +CG G Q R+R    P+  +GG+ C G   E R CN    CP +  W+ +G W  C+A CGGG Q      RRR   +G  C G   E +SC+TNPC      T
Sbjct:  598 WTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQA-----RRRICENGPDCAGCNVEYQSCNTNPCPELKKTT 708          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|85542049|sp|Q96RW7.2|HMCN1_HUMAN (RecName: Full=Hemicentin-1; AltName: Full=Fibulin-6; Short=FIBL-6; Flags: Precursor)

HSP 1 Score: 86.6557 bits (213), Expect = 1.368e-15
Identity = 46/115 (40.00%), Postives = 58/115 (50.43%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            +S +  W  CSV+CG G Q+R R+  QP   NGGK C+G  +E R C     CPV+  WS +  W  C+ +CG G QT  R          GRPC G   E   C+  PC   GA
Sbjct: 4532 FSQWSAWRACSVTCGKGIQKRSRLCNQPLPANGGKPCQGSDLEMRNCQ-NKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHGGRPCEGNAVEIIMCNIRPCPVHGA 4645          

HSP 2 Score: 75.8702 bits (185), Expect = 2.346e-12
Identity = 42/114 (36.84%), Postives = 59/114 (51.75%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGG-RQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFG 352
            WS++  W  CS SCG GTQ R R+   P    GG  C G   + + CN    CP++ +W+ +  WS+CS +CGGG RQ  R          GR C G   ++  C+++PC   G
Sbjct: 4646 WSAWQPWGTCSESCGKGTQTRARLCNNPPPAFGGSYCDGAETQMQVCNE-RNCPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCEGSDVQSDFCNSDPCPTHG 4758          

HSP 3 Score: 72.0182 bits (175), Expect = 3.463e-11
Identity = 46/119 (38.66%), Postives = 60/119 (50.42%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRR--NIRRRASGSGRPCLGEKAETRSCSTNPCSRFG 352
            VD  WS +  W  C+ SCG G Q R R    PS  +GG+ C G+A+E   CN    CPV+  WS +  W +CS +CG G QT  R  N    A G G  C G + + + C+   C   G
Sbjct: 4585 VDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHGGRPCEGNAVEIIMCNI-RPCPVHGAWSAWQPWGTCSESCGKGTQTRARLCNNPPPAFG-GSYCDGAETQMQVCNERNCPIHG 4701          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|1076761195|sp|D3YXG0.1|HMCN1_MOUSE (RecName: Full=Hemicentin-1; AltName: Full=Fibulin-6; Short=FIBL-6; Flags: Precursor)

HSP 1 Score: 85.8853 bits (211), Expect = 2.466e-15
Identity = 48/116 (41.38%), Postives = 60/116 (51.72%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            LWS+   W  CSV+CG G Q+R R+   P   NGG+ C+G   E+R C+    CPV+  WS +  W  CS +CG G QT  R      A   GRPC G   ET  C+  PC   G 
Sbjct: 4533 LWSA---WRPCSVTCGKGIQKRSRLCDNPPPANGGRPCQGADSEARHCH-NKLCPVDGHWSEWSFWEDCSRSCGHGNQTRTRTCSNPPAQHGGRPCEGHAVETIMCNIRPCPVHGV 4644          

HSP 2 Score: 71.633 bits (174), Expect = 4.132e-11
Identity = 43/114 (37.72%), Postives = 58/114 (50.88%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPC 348
            VD  WS +  W  CS SCG G Q R R    P + +GG+ C G A+E+  CN    CPV+  W+ +  WS+CS +CG G QT  R       S  G  C G + + + C+   C
Sbjct: 4584 VDGHWSEWSFWEDCSRSCGHGNQTRTRTCSNPPAQHGGRPCEGHAVETIMCNI-RPCPVHGVWNAWQPWSACSKSCGKGSQTRMRLCNNPPPSFGGAHCSGAETQMQVCNERHC 4696          

HSP 3 Score: 71.2478 bits (173), Expect = 6.563e-11
Identity = 39/118 (33.05%), Postives = 61/118 (51.69%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTNPCSRFG 352
            V  +W+++  WS CS SCG G+Q R R+   P  + GG  C G   + + CN    CPV+  W+ +  WS+C+ +CGGG +   R+         G  C G   ++  C+++PC   G
Sbjct: 4641 VHGVWNAWQPWSACSKSCGKGSQTRMRLCNNPPPSFGGAHCSGAETQMQVCNE-RHCPVDGRWATWSSWSACTVSCGGGARKRTRDCSDPVPQYGGNKCEGTGVQSDFCNSDPCPTHG 4757          

HSP 4 Score: 68.5514 bits (166), Expect = 3.620e-10
Identity = 45/144 (31.25%), Postives = 69/144 (47.92%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSP 382
            WS +  W  CS +C  G  +R R    P  +NGG++C G   + ++CN    CPV+  W  +  WS CS +CGGG +T +R       +  GR C G+  +   C+   C     +       + +DI+  I+   +TI T SP
Sbjct: 4759 WSPWSGWGTCSRTCNGGQMRRYRTCDNPRPSNGGRACGGPDTQIQRCNTD-MCPVDGSWGTWHSWSHCSVSCGGGERTRKRLCDNPVPTKGGRSCPGDATQVSRCNMQACPGGPQRARGSVIGNINDIEFGIAFLNATI-TDSP 4900          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|341942072|sp|Q8CG65.2|SSPO_MOUSE (RecName: Full=SCO-spondin; Flags: Precursor)

HSP 1 Score: 85.5001 bits (210), Expect = 2.681e-15
Identity = 181/783 (23.12%), Postives = 273/783 (34.87%), Query Frame = 0

Query:   17 RPTHKRLSRKPRRAE-----LDGKTSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQG-GSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGL----GWSIGKREY-----LKTGSHWHRSGLDTDEPWEGKWEEGV-RVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSC------RGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCE-----------------------------WSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC---GSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTL--AVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSC-GRGIQFRTRRVAV-TPKNGGSNCVGSNKDTRSCNVKSCNFFTTTTRPPTT 740
            R  H+  +R P RA      L    + TT      +  E   +      G     WS +S WS CS+SC GG++ R R     + QG G  C G + +   C  Q CP         NC  +   GA Y+ C           P + +  V+   R     +   G +    G    K  Y     L TG   H +G     P      +G     C+ G        + +D    V   W  + +W+ CS  C   T+ R R  + P+  +GG  C       G   +   C     CPV+  WS +G WSSC A C G     R       S  G+PCLG   ++R C              R+SS+   +          +P   P   +    S  +T    +    S     P   +             P+D   +   +   IP++   +  ++ SS + ++  E                             WS +  W  CS  CG G Q R+R    P       CPG A QSRIC+ H C   G  A +   R    ++G      +   G     S+       +  EG  Q ++   +     L + ++  ELG   A + +               Y +  G   D+   L   VE   G+   T   + GN   +  + + S    ++          V+  +  +G WS CS+SC G G + RTR+  + T   GG +C G  +D   C    C     +T  P T
Sbjct: 3040 RHRHRFCARPPHRAPFSLVLLTTVAAPTTLCPGPEAEEEPCLLPGCNQAG----GWSPWSPWSGCSRSCGGGLRSRTRACDQPSPQGLGDFCEGPQAQGEACQAQPCP-------VTNCSAME--GAEYSPC-------GPPCPRSCDDLVHCVWRCQPGCYCPLGKVLSADGAICVKPSYCSCLDLLTGKR-HHAGTQLMRP------DGCNHCTCMEGR------LNCTDLPCQVSGDWCPWSKWTACSQPCRGQTRTRSRACVCPAPQHGGSPCPEESGGTGVQHQMEACPNATACPVDGAWSPWGPWSSCDA-CLGQSYRSRVCSHPPISDGGKPCLGGYQQSRPC--------------RNSST---LCTDCGGGQDLLPCGQPCPHSCQDLSLGSTCQPGSAGCQSGCGCPPGQLSQDGLCV------FPVDCHCHFQPRAMGIPENRSRSVGSTLSSWESLEPGEVVTGPCDNCTCVAGILQCHEVPSCPGPGIWSSWGPWEKCSVSCGGGEQLRSRQCARP------PCPGLAQQSRICHIHVCRETGCPAGR-LYRECQPSDG-CPFSCAHVTGQVACFSERCK-EGCHCPEGTFQHHVACVQECPCVLTVLLLQ-ELGLASAALGS---------------YPTLLG---DEGKPLGPGVELLPGQMLQT---DCGNCSCVHGKLSCSMVECSR----------VHGSFGPWGMWSLCSRSCGGLGTRTRTRQCVLPTLAPGGLSCRGPLQDLEYCFSPECPGTAGSTVEPVT 3724          

HSP 2 Score: 75.8702 bits (185), Expect = 2.709e-12
Identity = 75/286 (26.22%), Postives = 113/286 (39.51%), Query Frame = 0

Query:  231 SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN-IRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD---QPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRN-GGNECPGRAFQSRIC 511
            SD+   VDC+ + +  WS CS SCG G   + R +L+    GG SC  D   S+ C   A CPV   W+ +G W++CS +CGGG Q+ +R+ +       G PC G   E   C+   C       E      N+++          +P   P  + L P +  + +                 +     +         +     A L D   Q I +           S    Q C WS +  W+ C + CG GV+ R RS  +P    GG+ C G   + + C
Sbjct: 2329 SDEDNCVDCVLAPWSGWSDCSRSCGLGLIFQHRELLRLPLPGG-SCLLDQFRSQSCFVQA-CPVAGAWAEWGPWTACSVSCGGGHQSRQRSCVDPPPKNGGAPCPGPSHEKAPCNLQLCPG-DTDCEPGLVHVNAELCQK-----GLVPPCPP--SCLDPEANRSCSGHCMEGCRCPPGLLLQDSHCLPLSECPCLVGQKLIQPRLAFLLDNCSQCICEKGTLLCKPGACS----QSCGWSAWSPWTACDRSCGSGVRARFRSPTNPPVAFGGSPCEGDRQELQAC 2600          

HSP 3 Score: 62.7734 bits (151), Expect = 2.255e-8
Identity = 47/119 (39.50%), Postives = 59/119 (49.58%), Query Frame = 0

Query:  235 GSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAI----ESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPC 348
            GS +C W+S+  W  CS SCG G QQR+     P   GG  C  D +    E R CN  A CPV   WS++  WS C  +CGGGR    R+         G  C+GE+   R C+  PC
Sbjct: 3790 GSGNCFWTSWAPWEPCSRSCGVG-QQRRLRAYHPPGPGGHWCP-DILTAYQERRFCNLRA-CPVPGGWSHWSPWSWCDRSCGGGRSLRSRSCSSPPPKNGGTSCVGERHHVRPCNPMPC 3905          

HSP 4 Score: 58.151 bits (139), Expect = 6.410e-7
Identity = 68/287 (23.69%), Postives = 113/287 (39.37%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSC---GTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSR--SSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVH-PSRNGGNECPGRAFQSRICNRHTC-GSGA 520
            W+ +  WS CS SC   G     R+R  L PS+     C G+A +   C+       +  W  +  WS+CSA+C GG QT  R+    A G+   CLG   +TR C+ +PC            ++    +    S+   +    P              +  T +   +    P      ++    +     +  ++ A      +  ++ +  +     +S +    C WS +  WS CS+ C VG++ R R+    P+  GG EC G    +  C+   C G GA
Sbjct: 2611 WTPWTSWSSCSQSCLVPGGDPGWRQRSRLCPSSRD-TFCPGEATQEEPCSPPVCPVPS-AWGLWASWSTCSASCNGGIQTRGRSCSGSAPGNPV-CLGPHTQTRDCNMHPC------------TAQCPGNMVFRSAEQCLEEGGPCPQLCL---AQDPGVECTGSCAPSCNCPPGLFLHNASCLPRSQCPCQLHGQLYAPGAVAHLDCNNCTCISGEMVCTSKRCPVACGWSPWTPWSPCSQSCNVGIRRRFRAGTEPPAAFGGAECQGPNLDAEFCSLRPCRGPGA 2879          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|81864798|sp|Q700K0.1|SSPO_RAT (RecName: Full=SCO-spondin; Flags: Precursor)

HSP 1 Score: 83.5741 bits (205), Expect = 1.222e-14
Identity = 117/506 (23.12%), Postives = 167/506 (33.00%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQG-GSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDD--------TGSVDCL---------WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIES------RQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSC--STNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSS-------------------IKRVQDCE----------WSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            W  +S WS CS+SC GG++ R R       QG G  C G + +   C  Q CP         NC  +   GA Y+ C                 P  +      +  W+            Y   G      G    +P          ++ + G +  P S     D         G ++C          W  + +W+ CS  C   T+ R R  + P+  +GG  C  +A E+        C     CPV+  WS +G WS C A C G     R       S  G PCLG   ++R C  S+ PC+  G   +                    +P   P   +    S  +T    ++   S     P   +             P D   +   K   IP++   +  ++ SS                   +  V  C           WS +  W  CS PCG G Q R+R    P       CPG A QSR C+ H C
Sbjct: 3240 WGPWSPWSGCSRSCGGGLRSRTRACDKPPPQGLGDFCEGPQAQGEACQAQPCP-------VANCSTIE--GAEYSPCG---------------PPCPRSCDDLVHCMWHCQ-------PGCYCPPGKVLSADGAICVQP-----HHCSCLDLLTGKRHHPGSQLMRPDGCNHCTCMEGRLNCTDLPCQVSGDWCPWSEWTACSQPCRGQTRTRSRACVCPAPQHGGAPCPEEAGETGVQHQMEACPNPTACPVDGAWSPWGSWSPCDA-CLGQSYRSRMCSHPPPSDGGTPCLGGHQQSRPCRNSSTPCTDCGGGQD-------------------LLPCGQPCPHSCQDLSLGSTCQPGSSGCQSGCGCPPGQLSQDGLCV------FPADCHCHFQPKAMGIPENQSRSVGSALSSWESLEPGEVVTGPCDNCTCVAGVLQCHEVPSCPGPGIWSSWGPWEKCSVPCGGGEQLRSRQCARP------PCPGLAQQSRTCHIHVC 3677          

HSP 2 Score: 80.4925 bits (197), Expect = 1.019e-13
Identity = 76/286 (26.57%), Postives = 115/286 (40.21%), Query Frame = 0

Query:  231 SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRN-IRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD---QPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVH-PSRNGGNECPGRAFQSRIC 511
            SD+   VDC+ + +  WS CS SCG G   + R +L+P   GG SC  D + S+ C   A CPV   W+ +G W  CS +CGGG Q+ +R+ +       G PC G   E   C+   C       E      N+++          +P   P  + L P +  + +                 +     +         +     A L D   Q I +  +     +  S    Q C WS +  W+ C   CG GV+ R RS  + P+  GG+ C G   + + C
Sbjct: 2478 SDEDDCVDCVLAPWSGWSDCSRSCGLGLIFQHRELLRPPLPGG-SCLLDQLRSQPCFVQA-CPVAGAWAKWGPWEPCSVSCGGGHQSRQRSCVDPPPKNGGAPCPGPSHEKVLCNLQLCPG-DTDCEPGRVHVNAELCQK-----GLLPPCPP--SCLDPEANRSCSGHCVEGCRCPPGLFLQDSHCLPLSECPCLVGQKLMQPGLAFLLDNCSQCICESGILLCKPAACS----QSCGWSAWSPWTACDHSCGSGVRARFRSPTNPPAAFGGSPCEGDRQELQAC 2749          

HSP 3 Score: 72.0182 bits (175), Expect = 3.810e-11
Identity = 76/292 (26.03%), Postives = 116/292 (39.73%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSC---GTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRV-------QDCEWSDYDTWSVCSKPCGVGVQYRNRS-IVHPSRNGGNECPGRAFQSRICNRHTC-GSGA 520
            W+ +  WS CS SC   G    +R+R  L PS+     C G+A +   C+  + CPV   W  +  WS+CSA+C GG QT  R+    A G+   CLG   +TR C+ +PC            ++    +    S+   +    P              +  T +     +  P      ++    +     +  ++ A     P     L   N + SS + V         C WS +  WS CS+ C VG++ R R+    P+  GG EC G    +  C    C G GA
Sbjct: 2760 WTPWTSWSSCSQSCLVPGGDPGRRQRSRLCPSSRD-TFCPGEATQEEPCSP-SLCPVPSAWGLWASWSACSASCNGGIQTRGRSCSGSAPGNPV-CLGPHTQTRDCNVHPC------------TAQCPGNMVFRSAEKCLEEGGPCPQLCL---AQDPGVECTGSCAPGCSCPPGLFLHNASCLPRSQCPCQLHGQLYA-----PGAVARLDCNNCTCSSGEMVCTSERCPVACGWSPWTPWSPCSQSCNVGIRRRFRAGTAPPAAFGGAECRGPNLDAEFCTLRPCQGPGA 3028          

HSP 4 Score: 65.4698 bits (158), Expect = 3.403e-9
Identity = 48/121 (39.67%), Postives = 61/121 (50.41%), Query Frame = 0

Query:  235 GSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAI----ESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPCSR 350
            GS +C W+S+  W  CS SCG G QQR+     P   GG  C  D +    E R CN  A CPV   WS++  WS C  +CGGGR    R+         G  C+GE+   RSC+  PC +
Sbjct: 3939 GSENCFWTSWAPWEPCSRSCGVG-QQRRLRAYHPPGPGGHWCP-DILTAYQERRFCNLRA-CPVPGGWSHWSPWSWCDRSCGGGRSLRSRSCSSPPPKNGGASCVGERHHVRSCNPMPCEK 4056          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|8134718|sp|Q62217.1|SEM5A_MOUSE (RecName: Full=Semaphorin-5A; AltName: Full=Semaphorin-F; Short=Sema F; Flags: Precursor)

HSP 1 Score: 81.6481 bits (200), Expect = 3.200e-14
Identity = 44/116 (37.93%), Postives = 57/116 (49.14%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKT 355
            W+ +  WS CS +CG G Q R+R    P+  +GG+ C G   E R CN    CP +  W+ +G W  C+A CGGG Q      RRR   +G  C G   E   C+TN C      T
Sbjct:  598 WTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQA-----RRRTCENGPDCAGSNVEYHPCNTNACPELKKTT 708          

HSP 2 Score: 67.0106 bits (162), Expect = 9.840e-10
Identity = 80/284 (28.17%), Postives = 116/284 (40.85%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPC 348
            W+ +  W  C+  C GG+Q R R       + G  C G   E   C+T  CP      P      VN+     ++  G Y+              +++T K R    N   +G    +R  ++  S    SG  TD         G+  + +   + S           +V+  WS++  WS+CS  C  G + RKR+   P    GG  C G ++E ++CN    CPV+  WS +  WS CSATCGGG     R+    A    G  CLG   E   C+T  C
Sbjct:  656 WTGWGPWERCTAQCGGGIQARRRTC-----ENGPDCAGSNVEYHPCNTNACPELKKTTPWTPWTPVNI-----SDNGGHYEQR------------FRYTCKARLPDPNLLEVG---RQRIEMRYCSSDGTSGCSTD---------GLSGDFLRAGRYS---------AHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNI-LPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTC 895          

BLAST of EMLSAG00000000316 vs. SwissProt
Match: gi|667467090|sp|B3EWY9.1|MLP_ACRMI (RecName: Full=Mucin-like protein)

HSP 1 Score: 81.6481 bits (200), Expect = 3.721e-14
Identity = 45/114 (39.47%), Postives = 65/114 (57.02%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASG-SGRPCLGEKAETRSCSTNPC 348
            +D  ++ +  WS CSV+CG G Q+R R   +P  +NGG+ C GD +E+R+C     CPV+  ++ +G WS CS TC  G Q   R+         GR C+G   ETR+C+  PC
Sbjct:  197 IDGNFTQWEIWSGCSVTCGKGVQRRFRSCTKPPPSNGGQDCIGDRLETREC-LKPPCPVDGNFTEWGAWSKCSQTCENGTQVRFRSCTNPPPAFGGRDCMGPTNETRACNDGPC 309          

HSP 2 Score: 73.559 bits (179), Expect = 9.259e-12
Identity = 47/123 (38.21%), Postives = 64/123 (52.03%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTNPCSRFGAKTE 356
            SVD  ++ +  +  CS SCG G Q R R    P   N G  C G   E+R CN G+ CP++  ++ +  WS CS TCG G Q   R+  +   S  G+ C+G++ ETR C   PC   G  TE
Sbjct:  139 SVDGGFTEWSPFGPCSTSCGPGIQVRFRNCTNPPPINNGSDCVGPRNETRPCNNGS-CPIDGNFTQWEIWSGCSVTCGKGVQRRFRSCTKPPPSNGGQDCIGDRLETRECLKPPCPVDGNFTE 260          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: gb|EEC16558.1| (F-spondin, putative [Ixodes scapularis])

HSP 1 Score: 84.7297 bits (208), Expect = 1.087e-15
Identity = 46/123 (37.40%), Postives = 65/123 (52.85%), Query Frame = 0

Query:  229 STSDDTGS--VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +TSD+  S  +DC+ + + QW  CS +CG G ++R+RMI     NGGK C    ++ R+C     CPV+C  + + +W  CS TCG G  Q   R I+R     G  C     E R C+  PC
Sbjct:  539 TTSDEPTSPVIDCVLTPWSQWGPCSKTCGNGRRERRRMIKLNPQNGGKPCPKKLVQRRRCKENPPCPVDCMLTPWSEWRPCSKTCGVGAVQERHRTIKRHPKNGGSTC-DATFERRYCTLPPC 660          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: gb|KFM73053.1| (Hemicentin-1, partial [Stegodyphus mimosarum])

HSP 1 Score: 79.7221 bits (195), Expect = 5.243e-14
Identity = 54/145 (37.24%), Postives = 74/145 (51.03%), Query Frame = 0

Query:  210 EEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFG 352
            +EGV   C G +    S+A+ +     V   WS++ +W +CS SCG G Q R+R    P+  NGGK+C G + E ++CN    C VN EW N+ +W  CSA+CG G R+  R+         G  C G   ET SC    C   G
Sbjct:  660 DEGVYA-CFGNNSLG-SAAAEAQLIVQVHGKWSNWQEWGQCSASCGDGEQSRQRFCNDPAPKNGGKTCIGLSKEVKKCNE-RPCSVNGEWGNWLEWERCSASCGVGQRKRQRKCDNPSPQYGGENCTGLDIETDSCILMKCPVDG 801          

HSP 2 Score: 74.7146 bits (182), Expect = 1.953e-12
Identity = 41/108 (37.96%), Postives = 59/108 (54.63%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETR 341
            SV+  W ++ +W RCS SCG G ++R+R    PS   GG++C G  IE+  C     CPV+  WS +  W  CS +CG G Q   R  +       G+PC+GE  E++
Sbjct:  741 SVNGEWGNWLEWERCSASCGVGQRKRQRKCDNPSPQYGGENCTGLDIETDSCIL-MKCPVDGRWSMWLDWQPCSVSCGKGAQIRIRECKNPEPLHGGQPCIGESEESQ 847          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: gb|KFM57644.1| (Hemicentin-1, partial [Stegodyphus mimosarum])

HSP 1 Score: 74.7146 bits (182), Expect = 6.823e-13
Identity = 47/111 (42.34%), Postives = 60/111 (54.05%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRR-RASGSGRPCLGEKAETRSC 343
            + D  W  +G W+ CS+SCG G + R R    P+  N GK C GD+ +S QC     CP + +WS++G+WSSCS TCG GG Q   R   R   S  G  C G   E  SC
Sbjct:   86 AADGNWGDWGAWTECSISCGEGVRHRYRACDNPAPQNRGKDCEGDSQQSEQCVNVRECPRDGKWSSWGKWSSCSVTCGRGGIQHRYRACNRPPPSAGGAHCKGLNHEQISC 196          

HSP 2 Score: 68.9366 bits (167), Expect = 4.158e-11
Identity = 40/85 (47.06%), Postives = 49/85 (57.65%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCG-TGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRR 320
            D  WSS+G+WS CSV+CG  G Q R R   +P  + GG  C+G   E   C A   CPV+ EWS++  WS CS TCG G Q   R
Sbjct:  146 DGKWSSWGKWSSCSVTCGRGGIQHRYRACNRPPPSAGGAHCKGLNHEQISCQAPGRCPVDGEWSDWTNWSPCSVTCGAGEQRRWR 230          

HSP 3 Score: 59.6918 bits (143), Expect = 4.169e-8
Identity = 34/105 (32.38%), Postives = 53/105 (50.48%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSC 343
            W  +  W+ CSV+CG G ++R R    P  + GGK C GD +E++ C     C  +  W ++G W+ CS +CG G +   R     A    G+ C G+  ++  C
Sbjct:   33 WGEWSVWNACSVTCGKGLRKRHRNCDSPRPSKGGKWCTGDHLETQVCEFPFRCAADGNWGDWGAWTECSISCGEGVRHRYRACDNPAPQNRGKDCEGDSQQSEQC 137          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: gb|EFA12053.2| (semaphorin-5c [Tribolium castaneum])

HSP 1 Score: 73.1738 bits (178), Expect = 4.815e-12
Identity = 86/328 (26.22%), Postives = 121/328 (36.89%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILA-RQGGSPCRGERKESRVCSTQ--QCPHFV-----------GDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPW------EGKWEEGVRVECVGGSKKSPSSASTSDDTGSVD-CL--------WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNC-EWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNS 364
            WS +S+WS CS +C   V+ R R     A   GG  C G+ +    C  +   CP              GD    +C          + C  R +V NN  P                      G  +  G  E  K     HR  + T  PW       G + +  R  C    K S    +  ++  + D C         WS++G W  CSV CG G Q R R         G  C G ++++R+CN       +C EW  + +WS C+A+CG G +T  R         G  C G   E   C   PC     ++ES H ++ S
Sbjct:  586 WSVWSAWSACSATCGTAVKTRSRTCTNPAPAHGGRVCVGQDRTEAFCDAEIPPCPAMPQDGGWGEWSDWGDCSPSHC----------SGCKRRTRVCNNPPP--------------------ENGGQFCAGNDEEFKFECPEHRKTVST--PWISLTNSSGDYNKRFRCTCKAPVKMSLIKVAMKEEVCNNDNCFTKDSEHPKWSAWGPWGECSVQCGGGLQNRTRYC------EGIGCTGPSVQTRECNKH-----HCREWGCWSEWSPCNASCGWGVKTRHRVCL------GSNCSGHDWEKEPCQNAPC-----ESESLHYNAPS 859          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: gb|EEC01160.1| (Semaphorin-5A, putative, partial [Ixodes scapularis])

HSP 1 Score: 72.7886 bits (177), Expect = 6.984e-12
Identity = 49/149 (32.89%), Postives = 72/149 (48.32%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNG-GKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGR---PCLGEKAETRSCSTNPCSRFGAKT----ESRHSSSNSDIDNSISSSFSTI 377
            +D  WS +G+WS+CSV CG G Q R+R+   P  +G G  C+G + E R CN       +  WS + ++S CS TCGGG    R     R  GSG     C GE  +   C   PC    A      E+R  S+   +++ + +  + +
Sbjct:  498 IDGDWSEWGEWSQCSVPCGGGVQLRERLCDSPRPSGKGAECQGPSREQRDCNIHE---CHSFWSCWSEYSECSVTCGGGGTKSRTRTCVREPGSGTESGACEGEATQVAPCDAAPCPTPLASVPRMQETREISTGIRVEHLVGACIACL 643          

HSP 2 Score: 67.0106 bits (162), Expect = 3.885e-10
Identity = 43/120 (35.83%), Postives = 59/120 (49.17%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPV--------NCEWSNFGQWSSCSATCGGGRQTXRRNIR--RRASGSGRPCLGEKAETRSCSTNPCS 349
            W+ +  WS CS SCG   + R+R    P   +GG+ C G   E   C++   CPV           WS +G WS CSA CGGG Q  RR     +   G G  C   +++ R+C+T+ CS
Sbjct:  314 WTEWSAWSACSQSCGIAVKSRRRTCSNPKPKHGGRVCLGPEQEQIYCSSNPPCPVPTTPAVTRQSSWSEWGAWSECSAQCGGGFQWRRRQCHSPKGRDGCGGGC---ESDYRACNTHACS 430          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: XP_016772940.1 (PREDICTED: coadhesin-like isoform X2 [Apis mellifera])

HSP 1 Score: 67.3958 bits (163), Expect = 9.235e-11
Identity = 39/115 (33.91%), Postives = 59/115 (51.30%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATC-PVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            WS++G WS C+  CG G + R R+   P  +N    C   A+E++ C  G  C  ++  WSN+ +W+ CS  CG G +T +R+     +  G  C G   E + C+ N CS  G 
Sbjct:  187 WSAWGIWSSCTAICGKGLKYRTRICNSPIPSNYKLICHDSALETKNC-LGLNCRKLSRTWSNWSKWTMCSVECGSGIRTRKRSCSEIQNLQGTSCKGVSKEIKGCTINNCSINGM 300          

HSP 2 Score: 65.4698 bits (158), Expect = 4.933e-10
Identity = 36/100 (36.00%), Postives = 56/100 (56.00%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAE 339
            WS++ +W+ CSV CG+G + RKR   +     G SC+G + E + C     C +N  WS++  WSSC+++CG G Q   R     + +G+G  C G  +E
Sbjct:  245 WSNWSKWTMCSVECGSGIRTRKRSCSEIQNLQGTSCKGVSKEIKGCTIN-NCSINGMWSSWTIWSSCTSSCGIGTQLRNRMCNNPSPNGNGTSCFGSASE 343          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: gb|KPM09935.1| (Thrombospondin repeats protein [Sarcoptes scabiei])

HSP 1 Score: 66.6254 bits (161), Expect = 9.450e-11
Identity = 46/138 (33.33%), Postives = 69/138 (50.00%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCS---------VSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-CLGEKAETRSCSTNPCSR-FGAKTESRHSS 361
            SV+  WS++G WS C          + CG GTQ+R R    PS  N G+ C G++I+  +CN     P+   WS++ QWS CS  C    Q  +R  +  +S  G   C+G+    ++CS   C + FG   + R+ S
Sbjct:  121 SVNGEWSNWGDWSPCQLNSNLIEQGIQCGIGTQKRIRHCNNPSPVNNGEFCHGESIQVSECNILCP-PIEGLWSSWTQWSICSQDC---TQIKKRICKNLSSQYGESNCIGQDFMIQNCSGGLCRKTFGKFNDDRNIS 254          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: EDO64815.2 (AGAP002268-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 68.1662 bits (165), Expect = 2.243e-10
Identity = 44/127 (34.65%), Postives = 61/127 (48.03%), Query Frame = 0

Query:  234 TGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMIL----QPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA--------SGSGRPCLGEKAETRSCSTNPC 348
            T  V   WS +G WS CS +CG+GTQ R R+ L     P+     +C G+ +E + C     CPVN  W  +  WS+CS +C     +  R+IR R+        S  G+ C+GE  E   C    C
Sbjct: 2076 TIDVPVSWSPWGAWSPCSATCGSGTQFRSRICLLVNGSPAHGERFNCVGENVELKACEL-LPCPVNGGWGEWTGWSNCSLSC-VSEFSGVRSIRHRSRACDKPAPSLGGKQCVGEAYEEEPCHVKYC 2200          

HSP 2 Score: 66.2402 bits (160), Expect = 1.005e-9
Identity = 38/114 (33.33%), Postives = 54/114 (47.37%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPC 348
            +D  W+++  W+ CS  CG G   R R    P   +GG  C G   E + C     C V+  WS + +WS C+ +CG G +  RR          G+PC+G+  E   CST  C
Sbjct: 2202 IDGGWTAWSSWTGCSEPCGFGRSLRMRSCSNPVPRHGGLPCDGAESEVKACKV-QECHVDGGWSEWERWSPCNKSCGKGIKKRRRYCNNPEPKAGGKPCVGQNVEIAQCSTKQC 2314          

HSP 3 Score: 63.5438 bits (153), Expect = 6.042e-9
Identity = 38/124 (30.65%), Postives = 58/124 (46.77%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGT------GTQQRKRMILQPSTN-GGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRAS-GSGRPCLGEKAETRSCSTNPCSRFGAKTE 356
            W  +  WS CS+SC +        + R R   +P+ + GGK C G+A E   C+    CP++  W+ +  W+ CS  CG GR    R+         G PC G ++E ++C    C   G  +E
Sbjct: 2143 WGEWTGWSNCSLSCVSEFSGVRSIRHRSRACDKPAPSLGGKQCVGEAYEEEPCHV-KYCPIDGGWTAWSSWTGCSEPCGFGRSLRMRSCSNPVPRHGGLPCDGAESEVKACKVQECHVDGGWSE 2265          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: XP_016772939.1 (PREDICTED: coadhesin-like isoform X1 [Apis mellifera])

HSP 1 Score: 66.2402 bits (160), Expect = 2.322e-10
Identity = 39/120 (32.50%), Postives = 58/120 (48.33%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCE------WSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            WS++G WS C+  CG G + R R+   P  +N    C   A+E++ C       +NC       WSN+ +W+ CS  CG G +T +R+     +  G  C G   E + C+ N CS  G 
Sbjct:  187 WSAWGIWSSCTAICGKGLKYRTRICNSPIPSNYKLICHDSALETKNCLG-----LNCRKLSTGTWSNWSKWTMCSVECGSGIRTRKRSCSEIQNLQGTSCKGVSKEIKGCTINNCSINGM 301          

HSP 2 Score: 65.0846 bits (157), Expect = 5.242e-10
Identity = 36/100 (36.00%), Postives = 56/100 (56.00%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAE 339
            WS++ +W+ CSV CG+G + RKR   +     G SC+G + E + C     C +N  WS++  WSSC+++CG G Q   R     + +G+G  C G  +E
Sbjct:  246 WSNWSKWTMCSVECGSGIRTRKRSCSEIQNLQGTSCKGVSKEIKGCTIN-NCSINGMWSSWTIWSSCTSSCGIGTQLRNRMCNNPSPNGNGTSCFGSASE 344          

BLAST of EMLSAG00000000316 vs. Select Arthropod Genomes
Match: XP_016772937.1 (PREDICTED: coadhesin-like isoform X1 [Apis mellifera])

HSP 1 Score: 66.2402 bits (160), Expect = 2.322e-10
Identity = 39/120 (32.50%), Postives = 58/120 (48.33%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCE------WSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            WS++G WS C+  CG G + R R+   P  +N    C   A+E++ C       +NC       WSN+ +W+ CS  CG G +T +R+     +  G  C G   E + C+ N CS  G 
Sbjct:  187 WSAWGIWSSCTAICGKGLKYRTRICNSPIPSNYKLICHDSALETKNCLG-----LNCRKLSTGTWSNWSKWTMCSVECGSGIRTRKRSCSEIQNLQGTSCKGVSKEIKGCTINNCSINGM 301          

HSP 2 Score: 65.0846 bits (157), Expect = 5.242e-10
Identity = 36/100 (36.00%), Postives = 56/100 (56.00%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAE 339
            WS++ +W+ CSV CG+G + RKR   +     G SC+G + E + C     C +N  WS++  WSSC+++CG G Q   R     + +G+G  C G  +E
Sbjct:  246 WSNWSKWTMCSVECGSGIRTRKRSCSEIQNLQGTSCKGVSKEIKGCTIN-NCSINGMWSSWTIWSSCTSSCGIGTQLRNRMCNNPSPNGNGTSCFGSASE 344          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|1129173223|gb|OLP82647.1| (Hemicentin-1 [Symbiodinium microadriaticum])

HSP 1 Score: 128.257 bits (321), Expect = 6.496e-26
Identity = 94/291 (32.30%), Postives = 128/291 (43.99%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLV--NVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVEC---VGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +DC+WS + +WS CS+SCNGG   R+R   + A  GG  C G   E  VC+ Q CP        V+C  V  +  G+  A+C G  ++    V  A E              W  GGL                                     EC     G  K+  + S ++    VDC WSS+ ++S CS +C  GT  RKR+   P +NGGK C GDA ES  CN    CP +C+WS + QW+ CS  CGGG+    R+I       G PC+G+  +   C+   C
Sbjct: 2120 VDCQWSLWDAWSACSRSCNGGTYTRQRAEQVPAAHGGRSCSGSADEDGVCNVQGCP--------VDCKWVPWSDWGSCSASCGGGTRMQTRVVEVAPE--------------W--GGL-------------------------------------ECETDASGGPKTQKTESCNEQPCPVDCQWSSWSKFSLCSKTCDAGTMSRKRLK-APESNGGKPCTGDAEESNFCNTQG-CPRDCQWSEWTQWTPCSKLCGGGKIKRFRDIAIPRKNGGEPCVGQAEQEADCNVLDC 2347          

HSP 2 Score: 92.4337 bits (228), Expect = 4.524e-15
Identity = 44/111 (39.64%), Postives = 60/111 (54.05%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            DC WS + QW+ CS  CG G  +R R I  P  NGG+ C G A +   CN    CPV CEW+++  WS C  TC GG +  +R+ +++    G PC G       C++ PC
Sbjct: 2294 DCQWSEWTQWTPCSKLCGGGKIKRFRDIAIPRKNGGEPCVGQAEQEADCNV-LDCPVACEWNDWSDWSVCPVTCDGGIRVKQRSKKQQEQSGGLPCAGNDTAREFCNSEPC 2403          

HSP 3 Score: 92.0485 bits (227), Expect = 6.837e-15
Identity = 49/133 (36.84%), Postives = 66/133 (49.62%), Query Frame = 0

Query:  216 ECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +C GG+  S      S DT  VDC W+ +  W  CS SCG G   R R +  P  +GGK C G   + R CN    CP++C  +++  W SCS +CG G++   R +   A   G PC G    +R C   PC
Sbjct: 1819 DCAGGNSTS-EKKYCSVDTCPVDCEWNDWQDWRGCSTSCGPGRGLRMRDVRTPMLHGGKDCEGGFSQERDCNL-RFCPLHCALADWSDWGSCSVSCGDGQEARERRVAVEADYGGIPCTGPTEASRGCFLAPC 1949          

HSP 4 Score: 86.2705 bits (212), Expect = 3.648e-13
Identity = 46/112 (41.07%), Postives = 61/112 (54.46%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            VDCL   + QWS CS++CG G+Q R R I      GGK C     E+  C+ G  CPV+C+WS +  WS+CS +C GG  T +R  +  A+  GR C G   E   C+   C
Sbjct: 2064 VDCLLGDWSQWSPCSMTCGKGSQTRLRGIRNGPAYGGKECDPKRNETVPCDLG-ICPVDCQWSLWDAWSACSRSCNGGTYTRQRAEQVPAAHGGRSCSGSADEDGVCNVQGC 2174          

HSP 5 Score: 83.9593 bits (206), Expect = 2.104e-12
Identity = 48/136 (35.29%), Postives = 70/136 (51.47%), Query Frame = 0

Query:  222 KKSPSSASTSDDTGS-------VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRG--DAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            K  PS+ ++   TG+        DC  S +  WS CSVSCG G  +R R +  P+  GG+ C G     E + C+   TCPV+CEW+++  W  CS +CG GR    R++R      G+ C G  ++ R C+   C
Sbjct: 1759 KPCPSNQTSIQQTGTCSLKQCPTDCKLSDWTLWSDCSVSCGPGQAERSRQVQVPALFGGRDCAGGNSTSEKKYCSVD-TCPVDCEWNDWQDWRGCSTSCGPGRGLRMRDVRTPMLHGGKDCEGGFSQERDCNLRFC 1893          

HSP 6 Score: 81.6481 bits (200), Expect = 1.106e-11
Identity = 43/111 (38.74%), Postives = 58/111 (52.25%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            DC W  +  W  CSV+CGTG  +R R   +   +GG  C G   + + C+  A CPV+C   ++ QWS CS TCG G QT  R IR   +  G+ C  ++ ET  C    C
Sbjct: 2009 DCEWDVWTDWEACSVTCGTGVSRRTRARKKYEKDGGHVCWGTEDDEQACSLEA-CPVDCLLGDWSQWSPCSMTCGKGSQTRLRGIRNGPAYGGKECDPKRNETVPCDLGIC 2118          

HSP 7 Score: 80.1073 bits (196), Expect = 2.590e-11
Identity = 38/112 (33.93%), Postives = 54/112 (48.21%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            V C W+ +  W  C+ SCG+G Q R R +       G  C G A +SR C +   CP +CEW  +  W +CS TCG G     R  ++     G  C G + + ++CS   C
Sbjct: 1951 VHCQWTDWTDWGECTSSCGSGQQARTRAVAVQPAYEGDLCEGFASDSRPCESLPVCPTDCEWDVWTDWEACSVTCGTGVSRRTRARKKYEKDGGHVCWGTEDDEQACSLEAC 2062          

HSP 8 Score: 79.7221 bits (195), Expect = 3.721e-11
Identity = 49/150 (32.67%), Postives = 75/150 (50.00%), Query Frame = 0

Query:  214 RVECVGGSKKSPSSASTSDDTG-------SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDA------IESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSR 350
            R E V  +    S + ++D+ G        VDC W  +  W  CS SCG GT+ + R++      GG  C  DA       ++  CN    CPV+C+WS++ ++S CS TC  G  + R+ ++   S  G+PC G+  E+  C+T  C R
Sbjct: 2146 RAEQVPAAHGGRSCSGSADEDGVCNVQGCPVDCKWVPWSDWGSCSASCGGGTRMQTRVVEVAPEWGGLECETDASGGPKTQKTESCNE-QPCPVDCQWSSWSKFSLCSKTCDAGTMS-RKRLKAPESNGGKPCTGDAEESNFCNTQGCPR 2293          

HSP 9 Score: 77.7962 bits (190), Expect = 1.532e-10
Identity = 37/112 (33.04%), Postives = 60/112 (53.57%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            + C  + +  W  CSVSCG G + R+R +   +  GG  C G    SR C   A CPV+C+W+++  W  C+++CG G+Q   R +  + +  G  C G  +++R C + P 
Sbjct: 1895 LHCALADWSDWGSCSVSCGDGQEARERRVAVEADYGGIPCTGPTEASRGCFL-APCPVHCQWTDWTDWGECTSSCGSGQQARTRAVAVQPAYEGDLCEGFASDSRPCESLPV 2005          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|1087804337|ref|XP_018670827.1| (PREDICTED: fibrillin-1-like [Ciona intestinalis])

HSP 1 Score: 126.716 bits (317), Expect = 1.180e-25
Identity = 97/300 (32.33%), Postives = 136/300 (45.33%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP---------HFVGDVPDVNCPLVNVI----GATYANCDGRYKVTNNK-VPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            ++C+WS +S ++ CS SC GG Q R R + I A  GG+ C G+  E+RVC+TQ CP          F  D   V+C   N       A  A C G     + +     +  P   H     +  WN   +    G +E         R      E   G          +G S  +  S   +D    V C W  +  W +CS SCG G+Q R+R +L   + GG +C G+A + + CN    CP++CEW+ +  W  CS TCGGG Q+ +R +  +A   G PC G   ETR CS N C
Sbjct:  362 VNCQWSSWSPFNSCSMSCGGGNQTRSRSVAISAFCGGNSCAGDPTETRVCNTQCCPVNCQWSSWTEF--DSCSVSCGGGNQTRSRSVAVSALCGGNSCAGDPRDTRVCNSLPCPIHCAWESWAQWNECNVTCGGGMQE---------RKRQVAVEAMHGG---------IGCSGFTSESRLCNDICCPVACEWEGWASWGKCSKSCGAGSQDRRRHVLVEESCGGATCAGNATQVQLCND-VCCPLDCEWTEWTGWERCSTTCGGGTQSRKREVATQAFCQGLPCTGATNETRECSLNCC 640          

HSP 2 Score: 100.523 bits (249), Expect = 1.203e-17
Identity = 83/287 (28.92%), Postives = 128/287 (44.60%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNE-------VNAALKDQPIPDD--DLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            C W+S+ +++ CSVSCG G Q R R +   +  GG SC GD  +++ CN    CPVNC+WS++  + SCS +CGGG QT  R+    A   G  C+G+  +TR C+T+ C         + SS +     S+S        S  +  + F    +     T T   +T         ++ T   + + +    N+       V+A         D  D    N+    I     C W  +  W+ C+  CG G+Q R R +   + +GG  C G   +SR+CN   C
Sbjct:  252 CQWTSWTEFNSCSVSCGGGNQSRSRTVAISAFCGGNSCAGDPTDTKVCNT-HCCPVNCQWSSWTAFDSCSVSCGGGNQTRSRSFAISAFCGGNSCVGDPTDTRVCNTHCC-----PVNCQWSSWSPFNSCSMSCGGGNQTRSRSVAISAFCGGNSCAGDPTETRVCNTQCCPVNCQWSSWTEFDSCSVSCGGGNQTRSRSVAVSALCGGNSCAGDPRDTRVCNSLPCPIH----CAWESWAQWNECNVTCGGGMQERKRQVAVEAMHGGIGCSGFTSESRLCNDICC 528          

HSP 3 Score: 100.523 bits (249), Expect = 1.355e-17
Identity = 94/303 (31.02%), Postives = 140/303 (46.20%), Query Frame = 0

Query:  218 VGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA-KTESRHSSSNSDIDNSISS-SFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDD--DLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
             G   K  SS S +    +V C W S+  W+RCS +CG GTQ R R   + ++ GG SC G + +SR CN G  CPV+C W ++G++ SCS  CGGG QT  R++  R+S  G  C G   ++++C+T  C    +  +  R+ S + +      + S S    SS   +    SST + T  T         T+ T   + S +      +      ++A         D  D    N     +    +C+WS +  +  CS  CG G Q R+RS    +  GGN C G    +R+CN H C
Sbjct:   63 CGSCNKPSSSRSCNTHCCAVSCRWGSWSGWARCSRNCGGGTQTRSRGYSRHASCGGSSCAGPSSQSRSCNTGC-CPVHCSWGSWGRYGSCSRNCGGGTQTRSRSVAVRSSCGGSGCSGLSTQSQTCNTQCCPVHCSWGSWGRYGSCSRNCGGGTQTRSRSVAIQSSCGGSGCSGSSTQSQTCNTQCCPRHCQWTSWTEFNSCSVSCGGGNQSRSRTVAISAFCGGNSCAGDPTDTKVCNTHCCPV----NCQWSSWTAFDSCSVSCGGGNQTRSRSFAISAFCGGNSCVGDPTDTRVCNTHCC 360          

HSP 4 Score: 90.8929 bits (224), Expect = 1.161e-14
Identity = 51/112 (45.54%), Postives = 67/112 (59.82%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            V+C WSS+  +  CSVSCG G Q R R     +  GG SC GD  ++R CN    CPVNC+WS++  ++SCS +CGGG QT  R++   A   G  C G+  ETR C+T  C
Sbjct:  306 VNCQWSSWTAFDSCSVSCGGGNQTRSRSFAISAFCGGNSCVGDPTDTRVCNT-HCCPVNCQWSSWSPFNSCSMSCGGGNQTRSRSVAISAFCGGNSCAGDPTETRVCNTQCC 416          

HSP 5 Score: 85.1149 bits (209), Expect = 6.590e-13
Identity = 92/314 (29.30%), Postives = 142/314 (45.22%), Query Frame = 0

Query:  214 RVECVGGSKKSPSSASTSDDTGS--VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNE-------VNAALKDQPIPDD--DLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
               C G S   PSS S S +TG   V C W S+G++  CS +CG GTQ R R +   S+ GG  C G + +S+ CN    CPV+C W ++G++ SCS  CGGG QT  R++  ++S  G  C G   ++++C+T  C R    T     +S      S+S        S  +  + F    +     T T   +T         ++ T   + + +    N+       ++A         D  D    N     +    +C+WS +  ++ CS  CG G Q R+RS+   +  GGN C G   ++R+CN   C
Sbjct:  113 HASCGGSSCAGPSSQSRSCNTGCCPVHCSWGSWGRYGSCSRNCGGGTQTRSRSVAVRSSCGGSGCSGLSTQSQTCNT-QCCPVHCSWGSWGRYGSCSRNCGGGTQTRSRSVAIQSSCGGSGCSGSSTQSQTCNTQCCPRHCQWTSWTEFNS-----CSVSCGGGNQSRSRTVAISAFCGGNSCAGDPTDTKVCNTHCCPVNCQWSSWTAFDSCSVSCGGGNQTRSRSFAISAFCGGNSCVGDPTDTRVCNTHCCPV----NCQWSSWSPFNSCSMSCGGGNQTRSRSVAISAFCGGNSCAGDPTETRVCNTQCC 416          

HSP 6 Score: 74.3294 bits (181), Expect = 1.421e-9
Identity = 44/108 (40.74%), Postives = 61/108 (56.48%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGSGRPCLGEKAETRSC 343
            +DC W+ +  W RCS +CG GTQ RKR +   +   G  C G   E+R+C+    CPV+C  S +  W SCSATCG  G +T  RNI R +   G+ C  +  +T +C
Sbjct:  586 LDCEWTEWTGWERCSTTCGGGTQSRKREVATQAFCQGLPCTGATNETRECSLNC-CPVDCVMSEWSVWGSCSATCGPHGYKTRTRNITRESQCGGQTCSNDLIQTSTC 692          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|957848767|ref|XP_014674902.1| (PREDICTED: SCO-spondin-like [Priapulus caudatus])

HSP 1 Score: 124.79 bits (312), Expect = 6.255e-25
Identity = 131/508 (25.79%), Postives = 195/508 (38.39%), Query Frame = 0

Query:   30 AELDGKTSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKK------------SPSSASTSD------DTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNE-VNAALKDQPIPDDDLST--TNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            +EL+   S   S + M S+    A  +  F  ++D  W  +S W++C+ SCNGG Q R R+  +  + GG PCRGE  E+R C   +C            P +   G+   + +  + V +     A+E   +                         ++    WH        PW  +W E   V C GG +              P    T++      D   VD +W S+  W RCSVSCG G   R R  + P  +GG +C G++ E   C++   CPV+  W ++ +W+ C+  CGGG     R       G G PC G+  E   C+T PC+  G   E  H  +N  +       + +     P +     +  T  T    T   +        +  +  T          +   V      +P    +  T   N    +I  V    W  +  W  C+  CG GVQ R RS   P  +GG ECPG    SR CN   C
Sbjct: 1297 SELESYGSFKESLEAMFSVESCSAAEILYFVVQVDGFWHPWSEWTECTVSCNGGKQARFREC-VEEQYGGVPCRGETTENRPCGNDKC------------PELKSYGSFKESLEAMFSVESCS---AAEILYF------------------------VVQVDGFWH--------PWS-EWTE-CTVSCNGGKQARFRECVEEQYGGVPCRGETTENRPCGNDKCPVDGVWRSWTSWERCSVSCGGGENSRSRTCIGP-FHGGAACDGNSSEWGNCSSNP-CPVDGAWLDWSEWTGCNVPCGGGTTRRNRTCVEPLYG-GYPCRGDPVEIHPCNTAPCAVDGVWEEWTH-WTNCTLSCGGGEQWHSRRCEGPYHGGADCAGPTNVTRACNTHNCAVDGRWRAWSDWSMCTLECGGGEGRRNRTCVEPLYGGRPCGGREKETRPCNEHPCAIDGV----WRPWAAWEACNVSCGGGVQMRRRSCFGP-LHGGAECPGSPHDSRRCNDQAC 1745          

HSP 2 Score: 92.0485 bits (227), Expect = 6.382e-15
Identity = 127/530 (23.96%), Postives = 189/530 (35.66%), Query Frame = 0

Query:  216 ECVGGSKKSPSSASTSDDTGS-------VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE-SRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCE----WSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNK----ISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
             CVG          T DD  S       VD  W  +  W  C+V+CG G Q R R    P  + G  C GDA + R CN    CP++ E   +  WS C+ TCGGG Q   R+      G G  CL   +ET+ C  N C   G   E S  +        S+ S       + P Y        T       T               T  T T  + +   + E   AL           + N + S I   Q C     W  +  WS C+  CG G  Y+NRS   P  + G +C G + + + CN H C        + V       ++   +   G  N     +   Y GR        E+L           ++L + G    Q     R +     +  +  +W        W D             +T  +++    R++   R T    +  +  +    P   + +WS + +WS CS +CG     R  RV     +GG +C G+  +  SC+  +C+
Sbjct:  808 HCVGPYHGGDPCNGTEDDGRSCHERECPVDGFWQEWSDWVSCNVTCGGGQQTRVRECQAP-LHDGIDCGGDAEQERSCND-QPCPIDGELEEWSDWSECTVTCGGGIQNRSRSCVGPFYG-GLECLAALSETQKCRENYCPIDGVWREWSEWTECVVTCGGSVQSRQRGC--TGPYYGGADCDGETLEEQVCNTNPCPVNGEWQEWGEWTECTLTCGSGSQDRERECTKALH-----GGFNCSGNATNSRICNTQFCPIDGVWRQWSDWSECNVTCGGGQSYKNRSCSGPYYS-GLDCQGPSTEHKSCNEHHCPV------DGVFTPWTDWSECSVTCGGGWQNHSRECVGTMYGGRPCPGNSSEERLCNENLCPTMCGMDLALQGLSTDQIGRKARME-----LKSSWTQWS------DWSD-----------CSSTCGSSAKQSRRRECIVRETTCVGSDGEARLCSLHPCPEDGQWSDWSKWSECSATCGSASHTR-YRVCEGRAHGGLDCAGAATERASCDSVACS 1297          

HSP 3 Score: 92.0485 bits (227), Expect = 6.713e-15
Identity = 157/712 (22.05%), Postives = 254/712 (35.67%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVR-VECV---GGSKKSPSSASTSDDTGSVDC-------------------LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNS-----DIDNSISSSFSTIPTSSP--------------LYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDC----EWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            +D  W ++S W  C+ +C GG Q R R+       G   C G+ ++ R C+ Q             CP+           DG  +  ++   W+          +NR    +   +G   G  E L   S   +   +   P +G W E     ECV   GGS +S     T    G  DC                    W  +G+W+ C+++CG+G+Q R+R   + + +GG +C G+A  SR CN    CP++  W  +  WS C+ TCGGG+    R+       SG  C G   E +SC+ + C   G  T     S  S        N       T+    P              L  T+         ++T            +S T  S  +  ++T     +    + + + I  +     ++  + +  +  C    +WSD+  WS CS  CG     R R +     +GG +C G A +   C+   C                          L +Y    +S    F+ E     +  I +F  Q D    W    E  +      N G++    +  +E       GV    + T    C N +      S    ++ +    +V + +  ++     + +G    W  + EW+ C+ SC  G Q R R   V  + GG  C G   + R C    C
Sbjct:  836 VDGFWQEWSDWVSCNVTCGGGQQTRVRECQAPLHDG-IDCGGDAEQERSCNDQP------------CPI-----------DGELEEWSD---WSECTVTCGGGIQNR----SRSCVGPFYGGLECLAALSETQKC-RENYCPIDGVWREWSEWTECVVTCGGSVQSRQRGCTGPYYGGADCDGETLEEQVCNTNPCPVNGEWQEWGEWTECTLTCGSGSQDRERECTK-ALHGGFNCSGNATNSRICNT-QFCPIDGVWRQWSDWSECNVTCGGGQSYKNRSCSGPYY-SGLDCQGPSTEHKSCNEHHCPVDGVFTPWTDWSECSVTCGGGWQNHSRECVGTMYGGRPCPGNSSEERLCNENLCPTMCGMDLALQGLSTDQIGRKARMELKSSWTQWSDWSDCSST---CGSSAKQSRRRECIVRETTCVGSDGEARLCSLHPCPEDGQWSDWSKWSECSATCGSASHTRYR-VCEGRAHGGLDCAGAATERASCDSVACSE------------------------LESYGSFKESLEAMFSVESCSAAE--ILYFVVQVD--GFWHPWSEWTECTVSC-NGGKQARFRECVEE----QYGGVPCRGETTENRPCGN-DKCPELKSYGSFKESLEAMFSVESCSAAEILYFVVQVDGF---WHPWSEWTECTVSCNGGKQARFREC-VEEQYGGVPCRGETTENRPCGNDKC 1470          

HSP 4 Score: 92.0485 bits (227), Expect = 6.770e-15
Identity = 91/310 (29.35%), Postives = 134/310 (43.23%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPD-----VNCPLVNVIGATYAN--CDGRYKVTNNKV-PWASERPVYKH--TRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC------SRFGAKTE 356
            +D  W D+S W+ C+  C GG   R R   +    GG PCRG+  E   C+T  C   V  V +      NC L    G  + +  C+G Y    +   P    R    H      R+  W+     WS+     L+ G    R      EP  G           GG +K   +   ++   ++D +W  +  W  C+VSCG G Q R+R    P  +GG  C G   +SR+CN  A CP++  W  +  W  C  +CGG  ++  R+      G G PC+G+  +TR+C+ +PC      S +G  TE
Sbjct: 1527 VDGAWLDWSEWTGCNVPCGGGTTRRNR-TCVEPLYGGYPCRGDPVEIHPCNTAPCA--VDGVWEEWTHWTNCTLSCGGGEQWHSRRCEGPYHGGADCAGPTNVTRACNTHNCAVDGRWRAWSD----WSMCT---LECGGGEGRRNRTCVEPLYGG-------RPCGGREKE--TRPCNEHPCAIDGVWRPWAAWEACNVSCGGGVQMRRRSCFGP-LHGGAECPGSPHDSRRCNDQA-CPIDGVWLEWAPWDICPVSCGGSEESRMRDCNGPYYGGG-PCVGKAQQTRACNDHPCPIDGKWSSWGGWTE 1814          

HSP 5 Score: 86.6557 bits (213), Expect = 2.895e-13
Identity = 95/350 (27.14%), Postives = 143/350 (40.86%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDG----RYKVTNNKVPWASERPVYKHTRK---NRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGS-GRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIP-TSSPLYTTLFPSSTTTTTITTTTTT 403
            +D +W  +S WS C+  C GG + R  +  +    GG PC G  KE+R C+   C      +  V  P      A   +C G    R +     +   +E P   H  +   ++    +   L W+      +  G        D + P+ G         CVG   K+  + + +D    +D  WSS+G W+ CS SCG G   R R+ + P  + GK C  +  E+ +CN    CP+  EW  +G W  CS +C GG ++ +R     + G    PC+G+K E   C+ + CS       +     N   D  IS  +   P    PL    FP    T T  T  T 
Sbjct: 1637 VDGRWRAWSDWSMCTLECGGG-EGRRNRTCVEPLYGGRPCGGREKETRPCNEHPCA-----IDGVWRPWA-AWEACNVSCGGGVQMRRRSCFGPLHGGAECPGSPHDSRRCNDQACPIDGVWLEWAPWDICPVSCGGSEESRMRDCNGPYYGG------GPCVG---KAQQTRACNDHPCPIDGKWSSWGGWTECSTSCGVGFVNRSRVCVGP-FHEGKLCSENHTETAECNE-TPCPIPGEWFEWGPWGRCSVSCMGGLRSRKRECDMDSYGEITAPCIGKKDEIGECNKHGCS-----VRNCADLVNKAQDEVISGYYGIDPDQEGPLQN--FPVWCDTLTDPTGITV 1961          

HSP 6 Score: 86.2705 bits (212), Expect = 3.760e-13
Identity = 74/280 (26.43%), Postives = 106/280 (37.86%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            VD +WS++  W  C+ SC  G   RKR  + P  +G K+C G  ++  +CN   +CPV+  W ++  W +C ATCGG  Q   R+ R    G GR C+G   E R+C+  PC                                +  +   F S    T   T+    S   +   S       TT   T   ++     A  D+  P D                   W  ++ W  C++ CG G + R R    P  NG  +C G    S  CN H C
Sbjct:  487 VDGIWSTWLDWMECNASCNGGVHMRKRDCVGPFYSG-KNCTGTNMDLAKCNT-HSCPVDGIWKDWQDWRACHATCGGALQFRSRHCRTPKYG-GRDCIGLSQEVRACNEEPCP------------------------------GNEAWLLEFSSWAPWTACNTSCGGGSQVRSRACSIPFAMRNTTRNCTGLFVEQR---ACNDEYCPVDG-----------------SWVTWNPWQACNRTCGGGRRLRTRLCYAPMYNGA-DCVGDNTDSESCNEHAC 712          

HSP 7 Score: 80.1073 bits (196), Expect = 2.576e-11
Identity = 78/285 (27.37%), Postives = 110/285 (38.60%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE-SRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTT-----TTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            D  W ++  W+ CSVSCG G   R R  + P  +GG  C G   + R C+    CPV+  W  +  W SC+ TCGGG+QT  R  +      G  C G+  + RSC+  PC   G   E S  S         I +   +     P Y  L   +  + T                 +  T    T   +  +            A  D    ++ +  TN    +       EW ++  W+ C+  CG G Q R R     + +GG  C G A  SRICN   C
Sbjct:  782 DGYWLAWQPWTPCSVSCGWGNHTRSRHCVGP-YHGGDPCNGTEDDGRSCHE-RECPVDGFWQEWSDWVSCNVTCGGGQQTRVRECQAPLH-DGIDCGGDAEQERSCNDQPCPIDGELEEWSDWSECTVTCGGGIQNRSRSC--VGPFYGGLECLAALSETQKCRENYCPIDGVWREWSEWTECVVTCGGSVQSRQRGCTGPYYGGADCDGETLEEQVCNTNPCPVNG------EWQEWGEWTECTLTCGSGSQDRERECTK-ALHGGFNCSGNATNSRICNTQFC 1054          

HSP 8 Score: 76.2554 bits (186), Expect = 4.237e-10
Identity = 116/477 (24.32%), Postives = 170/477 (35.64%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEE-----GVRVECVGGSK------------------KSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTT----TPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            W ++ SWS C+ SC  G + R R         G  C GE  ES+ C  + CP           P +          DG +       P +       HTR    +    GG        +         RS  + + P +G W+E        V C GG +                   +    S +D    +D     +  WS C+V+CG G Q R R  + P   GG  C     E+++C     CP++  W  + +W+ C  TCGG  Q+ +R       G G  C GE  E + C+TNPC   G   E    +  +    S S       T + L+     S   T +    T            +  S    +     +  N        + L  Q  P  +  + N     +  V    W+D   WS CS  CG G Q  +R  V  +  GG  CPG + + R+CN + C
Sbjct:  718 WREWESWSPCTVSCGRGHRHRSRGCNGAGD--GIQCPGETYESQPCRKRACPQP---------PPLGTASRIRPE-DGYWLAWQPWTPCSVSCGWGNHTRSRHCVGPYHGG--------DPCNGTEDDGRSCHERECPVDGFWQEWSDWVSCNVTCGGGQQTRVRECQAPLHDGIDCGGDAEQERSCNDQPCPIDGELEEWSDWSECTVTCGGGIQNRSRSCVGPFY-GGLECLAALSETQKCREN-YCPIDGVWREWSEWTECVVTCGGSVQSRQRGCTGPYYG-GADCDGETLEEQVCNTNPCPVNGEWQEWGEWTECTLTCGSGSQDRERECTKA-LHGGFNCSGNATNSRICNTQFCPIDGVWRQWSDWSECNVTCGGGQSYKNRSCSGPYYSGLDCQG-PSTEHKSCNEHHCPVDGVFT-PWTD---WSECSVTCGGGWQNHSRECVG-TMYGGRPCPGNSSEERLCNENLC 1164          

HSP 9 Score: 72.4034 bits (176), Expect = 5.231e-9
Identity = 169/741 (22.81%), Postives = 256/741 (34.55%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEE--GVRVECVGGSKKSPSSASTSDDTGSVDCL-----------------------WSSYGQWSRCSVSCGTGTQQRKRMILQPST--NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE------------------SRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRV--QDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRH-------------------TCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLY-IXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETC------IHKTAQEWKYASRAGVWKDDDPTLAVECFNG------ETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            WSD+  WS+CS +C GG + R R    + +     C+G   E + C+T    HF        CP V+ I +T+ +        N  V          H RK   +     G   +    +  K  +H        D  W+  W++       C GG+ +  S    +   G  DC+                       +SS+  W+ C+ SCG G+Q R R    P    N  ++C G  +E R CN    CPV+  W  +  W +C+ TCGGGR+  R  +      +G  C+G+  ++ SC+ + C   G   E                  SR  +   D       ++ + P          P  T +          +    TP S +      T +       + V       P       T ++ RS  +R    D  W ++  W  C+  CG G Q R R    P  + G +C G A Q R CN                     TCG G        +    G   L+    L    K  ++Y       RE  E    +        S      GP  G          ++ C      ++   QEW      G W +      + C +G      E T   +  F      S   T S    TQ   I    +GV  +WS   +WS C+ +CG G  ++ R  +  P   G +C G + + +SCN   C
Sbjct:  439 WSDWEDWSECSATCGGGGRNRSRTCNGVVKD----CKGNYTEIQECNT----HF--------CP-VDGIWSTWLDWMECNASCNGGV----------HMRKRDCVGPFYSGKNCTGTNMDLAKCNTH----SCPVDGIWK-DWQDWRACHATC-GGALQFRSRHCRTPKYGGRDCIGLSQEVRACNEEPCPGNEAWLLEFSSWAPWTACNTSCGGGSQVRSRACSIPFAMRNTTRNCTGLFVEQRACND-EYCPVDGSWVTWNPWQACNRTCGGGRRL-RTRLCYAPMYNGADCVGDNTDSESCNEHACPDAGTWREWESWSPCTVSCGRGHRHRSRGCNGAGDGIQCPGETYESQPCRKRACPQPPPLGTASRIRPEDGYWLAWQPWTPCSVSCGWGNHTRSR------HCVGPYHGGDPCN----GTEDDGRSCHERECPVDGFWQEWSDWVSCNVTCGGGQQTRVRECQAPLHD-GIDCGGDAEQERSCNDQPCPIDGELEEWSDWSECTVTCGGGIQNRSRSCVGPFYGG--LECLAALSETQKCRENYCPIDGVWREWSEWTECVVTCGGSVQSRQRGCTGPYYGGADCDGETLEEQVCNTNPCPVNGEWQEW------GEWTE----CTLTCGSGSQDRERECTKALHGGF----NCSGNATNSRICNTQFCPI----DGVWRQWS---DWSECNVTCGGGQSYKNRSCS-GPYYSGLDCQGPSTEHKSCNEHHC 1109          

HSP 10 Score: 70.8626 bits (172), Expect = 1.838e-8
Identity = 81/318 (25.47%), Postives = 115/318 (36.16%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP--HFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSI---------GKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSAST---------------------SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +D  WS +  W +C+ SCNGGV  R+R   +     G  C G   +   C+T  CP      D  D          A +A C G  +  +            +H R  +Y   +  GL   +         G   +L   S W         PW           C GGS+    + S                      +D+   VD  W ++  W  C+ +CG G + R R+   P  N G  C GD  +S  CN  A CP    W  +  WS C+ +CG G +   R      +G G  C GE  E++ C    C
Sbjct:  487 VDGIWSTWLDWMECNASCNGGVHMRKRDC-VGPFYSGKNCTGTNMDLAKCNTHSCPVDGIWKDWQDWR--------ACHATCGGALQFRS------------RHCRTPKYGGRDCIGLSQEVRACNEEPCPGNEAWLLEFSSW--------APWTA-----CNTSCGGGSQVRSRACSIPFAMRNTTRNCTGLFVEQRACNDEYCPVDGSWVTWNPWQACNRTCGGGRRLRTRLCYAPMYN-GADCVGDNTDSESCNEHA-CPDAGTWREWESWSPCTVSCGRGHRHRSRGC--NGAGDGIQCPGETYESQPCRKRAC 766          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|1087804328|ref|XP_018670824.1| (PREDICTED: fibrillin-1-like [Ciona intestinalis])

HSP 1 Score: 119.783 bits (299), Expect = 1.500e-23
Identity = 93/298 (31.21%), Postives = 135/298 (45.30%), Query Frame = 0

Query:   65 CKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP---HFVGDVPDVNCPLVNVIG--------ATYANCDGRYKV---TNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            C+W  ++ ++ CS SC GG Q R R + + A  GG+ C G+  ++RVC+T+ CP    +    P  +C +    G        A  A C G       T+ +V   +  P   H     +  WN   +    G +E         R      E   G          +G S  +  S S +D    V C W  +  W  CS SCG G+Q R+R +L   + GG +C G+A + + CN    CP++CEW+ +  W  CS TCGGG Q+ +R +  +A   G PC G   ETR CS N C
Sbjct:  309 CEWWGWTEFNSCSVSCGGGNQTRSRSVAVSALCGGNSCAGDPTDTRVCNTECCPVNCQWSSWSPFNSCSMSCGGGNQTRSRSVAISAFCGGNSCAGDPTDTRV--CNSLPCPIHCAWGSWAQWNECNVTCGGGMQE---------RKRQVAVEAMHGG---------IGCSGFTSESRSCNDICCPVACEWEGWASWGECSKSCGAGSQDRRRHVLVEESCGGATCAGNATQVQLCND-VCCPLDCEWTEWTGWERCSTTCGGGTQSRKREVATQAFCQGLPCTGATNETRECSLNCC 585          

HSP 2 Score: 93.5893 bits (231), Expect = 1.921e-15
Identity = 85/306 (27.78%), Postives = 134/306 (43.79%), Query Frame = 0

Query:  218 VGGSKKS-PSSASTSDDTGSV--DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSR----FGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
             GGS+ S P++ S   +T      C W  +  ++ CSVSCG GTQ R R +   +  GG SC G+  ++R CN    CPV+C W N+G++ SCS  CGGG QT  R++  + S  G  C G   +++ C+   C R    +G    +  S S    + + S S +             P+ T              ++ +P ++ + S      T +  +   ++A         D   T     +S+     C W  +  W+ C+  CG G+Q R R +   + +GG  C G   +SR CN   C
Sbjct:  173 CGGSRCSGPATQSQGCNTQCCPRHCEWWGWTGFNACSVSCGGGTQSRSRSVAVSALCGGNSCPGNPTDTRVCNT-QCCPVHCSWGNWGRYGSCSRNCGGGTQTRSRSVAIQPSCGGSGCPGSTVQSQRCNAQCCPRHCEWWGWTEFNSCSVSCGGGNQTRSRSVAVSALCGGNSCAGDPTDTRVCNTECCPVNCQWSSWSPFNSCSMSCGGGNQTRSRSV--AISAFCGGNSCAGDPTDT--RVCNSLPCPIHCAWGSWAQWNECNVTCGGGMQERKRQVAVEAMHGGIGCSGFTSESRSCNDICC 473          

HSP 3 Score: 74.3294 bits (181), Expect = 1.266e-9
Identity = 44/108 (40.74%), Postives = 61/108 (56.48%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGSGRPCLGEKAETRSC 343
            +DC W+ +  W RCS +CG GTQ RKR +   +   G  C G   E+R+C+    CPV+C  S +  W SCSATCG  G +T  RNI R +   G+ C  +  +T +C
Sbjct:  531 LDCEWTEWTGWERCSTTCGGGTQSRKREVATQAFCQGLPCTGATNETRECSLNC-CPVDCVMSEWSVWGSCSATCGPHGYKTRTRNITRESQCGGQTCSNDLVQTSTC 637          

HSP 4 Score: 72.0182 bits (175), Expect = 6.995e-9
Identity = 42/110 (38.18%), Postives = 61/110 (55.45%), Query Frame = 0

Query:  239 CLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            C W S+  +S CS SCG G Q + R + + +  GG  C G A +S+ CN    CP +CEW  +  +++CS +CGGG Q+  R++   A   G  C G   +TR C+T  C
Sbjct:  141 CRWGSWSVFSSCSRSCGGGIQTKTRSVARSAYCGGSRCSGPATQSQGCNT-QCCPRHCEWWGWTGFNACSVSCGGGTQSRSRSVAVSALCGGNSCPGNPTDTRVCNTQCC 249          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|942139375|ref|XP_014342845.1| (PREDICTED: SCO-spondin [Latimeria chalumnae])

HSP 1 Score: 118.627 bits (296), Expect = 5.405e-23
Identity = 128/529 (24.20%), Postives = 206/529 (38.94%), Query Frame = 0

Query:  229 STSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQ-PSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRAS-GSGRPCLGEKAETRSCSTNPCSRFGAK------TESRHSSSNSDIDNSISSSFSTIPTSSPLY------TTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVH-PSRNGGNECPGRAFQSRICNRHTC-GSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAG----IRNDGQETC-------IHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNG-GSNCVGSNKDTRSCNVKSCN 729
            S  + +  VDC WS++  W+ C  SCG G QQR R     P+TNGG  C GD ++ ++C +  + PV   WS +  WS+C+++C  G QT  R          G  C G   +TR C+T PC     K       E  HSS  +     I    + +  ++  Y        LF  + +   +             P  T +       T        E+    +  P+                   DC WS +  WS CSK C VG + R R+  + P+  GG +C G A     C+   C GS ++                      G +++ S    G +    + R ++ +    S+  + N+   G  +     G       DG  TC       ++ T +   Y  +  V ++D+     +C   E        +   + + R     +  + Q+S        VN +WS +  WS CS SCG G+Q R R     P +G G  C+G  ++ + CN+++C+
Sbjct: 2875 SCDESSCPVDCSWSAWSPWTPCDKSCGVGVQQRYRSPSNPPATNGGSPCVGDTVDVQECQSSCS-PVAGGWSFWSPWSTCTSSCDSGVQTRNRTCTNPGPVYGGADCPGPHIQTRDCNTQPCQEACPKNMVYRSAEECHSSGGACPRVCI-DLMANVECTTACYDGCYCDEVLFLQNDSCVPLNECMCYHLGKLYEPGETVSWDRCNNCTCAF----GEMTCGTEPCPV-------------------DCGWSGWTQWSTCSKTCDVGARRRYRAGTNPPAALGGRQCEGFAMDLEFCSMQPCKGSWSN---------------------WGPWSECSVPCGGGY----KNRTRISVFSRNSEFATCNIHRCGAHMQSCPKGKVWKACADGPVTCMDLSLKALNTTCEPGCYCPKETVLQNDECVPVSDCPCAENGVI----YSPGETVLRDCNNCSCVSGQISNCTEVICDVNGDWSAWTPWSECSASCGTGLQSRYRFCTDPPPSGNGLPCIGPEREGQPCNMQTCS 3349          

HSP 2 Score: 95.9005 bits (237), Expect = 4.332e-16
Identity = 98/381 (25.72%), Postives = 146/381 (38.32%), Query Frame = 0

Query:  231 SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRR-RASGSGRPCLGEKAETRSCSTNPCSR---------FGAKTESRHSS------SNSDIDNSISSSFSTIP---TSSPLYT------------------TLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIK---RVQDCE---------WSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHTCGSG---------ADQC------CERVIVKANGNAKLDQSLYLGAY 546
            SD+   VDC+ S +  WS CS +CG G   R+R +L+ +   G+ C     +SR C   A CPVN  WS +  WS C A C GG +  +R+         G  CLG+  +T  C+  PC           + A TE   +S      S  D+++  + S S I      S LY                      P+        +         +   S+     + +  +  TP D      ++ +     +   TN     +     VQ+C+         WS +  WS C+  C  GVQ RNR+  +P    GG +CPG   Q+R CN   C            A++C      C RV +    N +   + Y G Y
Sbjct: 2662 SDEINCVDCILSPWTSWSDCSHTCGLGVIFRQREVLRHALPEGE-CDEANFDSRSCFIQA-CPVNGLWSEWSDWSECDAKCNGGVRIKKRSCTDPPPKNGGHDCLGDAVQTELCNLQPCGDSQECGPEMIYVAATECEKNSLPPCPLSCKDLNSQTNCSSSCIEGCRCPSGLYLQEGHCINVSECYCYYSAKVRQPAEIFIKDNCSQCVCQDGIISCDESSCPVDCSWSAWSPWTPCDKSCGVGVQQRYRSPSNPPATNGGSPCVGDTVDVQECQSSCSPVAGGWSFWSPWSTCTSSCDSGVQTRNRTCTNPGPVYGGADCPGPHIQTRDCNTQPCQEACPKNMVYRSAEECHSSGGACPRVCIDLMANVECTTACYDGCY 3040          

HSP 3 Score: 85.8853 bits (211), Expect = 5.059e-13
Identity = 122/492 (24.80%), Postives = 192/492 (39.02%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVC--STQQCP-------HFVGDVPDVNCP------LVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHW-HRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSS---------SNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQ----------DCEWSDYDTWSVCSKPC-GVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHTC 516
            +D  WS +  WS CS      V+ RE      AR GG PC GE K+SR C  +T  C        HF    P   CP       ++ +    + C         +V    +  V     + R  + N+     ++G  E     S W  R+  +  EP      E V+  C+  + ++     TSD    ++  WSS+ QW+ CS SC  GT+ R R    P+  NGGK C G + + R+CN    C  +  WS +  WS+C+ TCGGG Q   R   +        C G   ++++C+T  C   G   +  +           S + ++N +                ++  + +            T       +T +S +    +    +      +L D+ +P D  +  N S  + +  Q          D   S + TWS CS  C G+G   R+RS  +P+  NGG  C G   + + C    C
Sbjct: 3553 VDGGWSSWGLWSPCSGCSGASVRMRE-CTNPPARFGGLPCEGETKQSRGCHDNTTVCSDCGGGQVHFECGKP---CPRSCDDLHISTVCLDSSECQPSCGCLTGQV---LQDGVCIEPAECRCKYQNS-----TLGIPEAENRTSAWTDRASWEYIEP-----GETVQAPCLNCTCETGHLNCTSDPLCRLNGGWSSWSQWTPCSQSCRGGTRYRFRDCNNPAPQNGGKGCLGISQQQRECNLQG-CDESKVWSEWSPWSACTVTCGGGEQMRTRTCLQLE------CQGLPVQSKACNTQVCFEVGCPVDRLYRECLNGEGCPYSCAHLNNRMDCFTDGCEEGCHCPVDMYLHNGSCVKDCPCILDRETLQQFQNHSTNSSESPVVLSGVRKV-----ISLGDEILPGDG-AYHNCSYCTCQHGQLNCTFHLCPVDGGMSLWSTWSTCSLSCGGLGQMTRSRSCTNPAPANGGKGCSGPTAEIKYCQTPEC 4014          

HSP 4 Score: 80.4925 bits (197), Expect = 2.328e-11
Identity = 73/283 (25.80%), Postives = 110/283 (38.87%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDN--SISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTP----IDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            ++  + +WS CS +CG G   R R         G SC G+A++ ++C+    CPV+C  S + +WS CS+TCGGG  T  R I   A   G PC       + C+T  C+      +   S +N+   +   + +    +     L             +  +      +    T     S TT       P    I +E N+ +           T N S  +     DC WS++  WS CS  CG GVQ   R         G EC G + + + C    C
Sbjct: 4927 VYGPWSEWSECSATCGGGHMTRNRTC--EDIADGLSCAGEAVQMKECST-HPCPVDCVVSKWSEWSECSSTCGGGVATKNRTIAAPAEHGGEPCPLLLRLQKPCNTQNCTPECPGNQISSSCANACPHSCTDLQTGAKCLQDDCELGCACPSDQVLQDGVCVSPQECPCSLIASTIPWLISLTTEDRLKIYPAGAVIYHECNSCICQN-------GTFNCSEDNCNV--DCAWSEWSPWSPCSVTCGSGVQTSARYQTQQRMYEGKECIGPSSRKQPCTLSDC 5197          

HSP 5 Score: 75.485 bits (184), Expect = 6.741e-10
Identity = 79/297 (26.60%), Postives = 113/297 (38.05%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPS-TNGGKSC---------RGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNP--CS-------RFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNE-VNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS-RNGGNECPGRAFQSRICNRHTC 516
            W  + +W+ CS +CG     R R    P   NGG+SC          G  +E +QC + + CPV+  WS++G WS CS  C G     R      A   G PC GE  ++R C  N   CS        F        S  +  I      S    P+   L   +                 ++T   P +   TS  T   +       E V A   +       L+ T++    +       WS +  W+ CS+ C  G +YR R   +P+ +NGG  C G + Q R CN   C
Sbjct: 3490 WCEWSEWTPCSKTCGYEMISRYRTCSCPKPQNGGESCVGIEKYYGETGVQLERKQCPSVSFCPVDGGWSSWGLWSPCSG-CSGASVRMRECTNPPARFGGLPCEGETKQSRGCHDNTTVCSDCGGGQVHFECGKPCPRSCDDLHISTVCLDSSECQPSCGCLTGQVLQDGVCIEPAECRCKYQNSTLGIPEAENRTSAWTDRASWEYIEPGETVQAPCLNCTCETGHLNCTSDPLCRLNG----GWSSWSQWTPCSQSCRGGTRYRFRDCNNPAPQNGGKGCLGISQQQRECNLQGC 3781          

HSP 6 Score: 71.2478 bits (173), Expect = 1.567e-8
Identity = 79/292 (27.05%), Postives = 116/292 (39.73%), Query Frame = 0

Query:  241 WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSP---LYTTLFPSSTTTTTITTTTTTTSTTTTT-----PTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTT------NNSRSSIKRVQDCEWSDYDTWSVCSKPCGV-GVQYRNRSIVHPSRNG-GNECPGRAFQSRICNRHTC 516
            WS +GQWS CS SCG G Q R R       +  + C+G+  +++QC   A CPV+ EWS +  WS+C+  C G        IRRR       C+  +   R CS  P S          +S   DI   +      + TS P   +     P   T + +T                 P      S        + P + E       Q +  +    T         R S +   +C WS + +W  C  PCGV  VQ+  RS  +P+++G G +C G   ++R C  + C
Sbjct: 1709 WSPWGQWSECSRSCGAGAQTRLRSCTSQPEDVLRHCQGEESQTQQCFTVA-CPVDGEWSEWTSWSNCTRNCSG------VVIRRRE------CIPPQNGGRHCSALPGS----------TSVTLDIKPCLLEGCPNV-TSCPGDLVTRDCAPCPLTCSDLTNKANCEEDMPCISGCWCPKGLVLDSDHRCVHPEDCPCEAEGVKYWPGQLVKVNCQICTCQKGRLEQCRQSPECSVNCGWSSWSSWGDCLGPCGVQSVQWSFRSPNNPTKHGNGKQCRGIYRKARRCQTNPC 1976          

HSP 7 Score: 66.6254 bits (161), Expect = 3.962e-7
Identity = 44/128 (34.38%), Postives = 57/128 (44.53%), Query Frame = 0

Query:  224 SPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPST-NGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCL--GEKAETRSCSTNPC 348
            +P      D    VD  W+ +  WS C V+CG GTQ R R  + P   N G  C G   E++ C+    CPV+  W  +  W+ CS +CG G  T RRN       SG       E  E + C   PC
Sbjct: 4391 TPEGTYCLDIDCKVDGTWTPWSPWSDCPVTCGQGTQIRNRACINPPPRNNGSDCEGPETEAQNCST-LPCPVDGGWCEWSDWTPCSQSCGPGIVTRRRNCSCLLLESGGAVCSDSESIEMQHCYNQPC 4517          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|1129209234|gb|OLQ10778.1| (Hemicentin-1 [Symbiodinium microadriaticum])

HSP 1 Score: 116.701 bits (291), Expect = 1.789e-22
Identity = 89/293 (30.38%), Postives = 122/293 (41.64%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGA------TYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRV-ECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +DC+W D+S WS C++SC GGV  R+R + +    GG PC G+ ++   C+            DV+CPL    G         A+CDG  +          E+PV                          L+  +  H   L          E+GV V EC             +    S DC WS +  W  C+VSC  G + R R +  P+  GG  C GDA E   CN  A CPV+C  S++  W  C+ +CG G Q  RR    +A+G G  C     E +SC   PC
Sbjct:  798 VDCQWYDWSPWSFCTKSCGGGVSKRKRTVAVKETTGGHPCEGDEEDLGRCN------------DVDCPLDCQWGDWTSWSDCTASCDGGVR--------QREKPVI-------------------------LEAKAQGHACSL----------EDGVEVGEC-------------NTQRCSRDCEWSDWADWGACTVSCSGGQKARTRGVKIPAVEGGADCSGDASEEMSCNLRA-CPVDCVPSDWSSWEPCTTSCGNGTQVRRRYAEVQATGGGLDCDNNFLEEQSCYEGPC 1021          

HSP 2 Score: 89.3521 bits (220), Expect = 4.058e-14
Identity = 46/130 (35.38%), Postives = 68/130 (52.31%), Query Frame = 0

Query:  222 KKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPC-LGEKAETRSCSTNPCSR 350
            +K  +S  T+    +VDC W  +  WS C+ SCG G  +RKR +    T GG  C GD  +  +CN    CP++C+W ++  WS C+A+C GG +   + +   A   G  C L +  E   C+T  CSR
Sbjct:  783 EKDQASRDTNSSIEAVDCQWYDWSPWSFCTKSCGGGVSKRKRTVAVKETTGGHPCEGDEEDLGRCN-DVDCPLDCQWGDWTSWSDCTASCDGGVRQREKPVILEAKAQGHACSLEDGVEVGECNTQRCSR 911          

HSP 3 Score: 83.1889 bits (204), Expect = 2.570e-12
Identity = 41/116 (35.34%), Postives = 65/116 (56.03%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGT----GTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            V C+W  +G WS C  SCG+     +  R+R +++  + GG  C+GDA   ++C     CPV+C W+++G+W +C+ +CGGG     RN    A+  G  C G   +  +CS+ PC
Sbjct: 1023 VSCVWGDWGSWSSCGASCGSLDSYVSSIRERDVVE--SAGGLPCQGDATRRKRCGFD-ECPVDCRWNDWGEWGACTRSCGGGLSERLRNASVAAAFGGSDCEGATRDVMACSSKPC 1135          

HSP 4 Score: 68.1662 bits (165), Expect = 1.020e-7
Identity = 38/121 (31.40%), Postives = 58/121 (47.93%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTES 357
            VDC W+ +G+W  C+ SCG G  +R R     +  GG  C G   +   C++   CPV+C  S++  W+ CS  CG G     R+    A   G+ C     E ++C  + C+   A  +S
Sbjct: 1081 VDCRWNDWGEWGACTRSCGGGLSERLRNASVAAAFGGSDCEGATRDVMACSS-KPCPVDCVLSDWTPWTECSHHCGNGSALRYRSEAHAAEYGGKACPETSHEVQACMGSDCTPTSALIKS 1200          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|762092679|ref|XP_011428773.1| (PREDICTED: SCO-spondin-like isoform X3 [Crassostrea gigas])

HSP 1 Score: 115.546 bits (288), Expect = 3.809e-22
Identity = 88/283 (31.10%), Postives = 123/283 (43.46%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD-QPI--PDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            VD +W+ + QWS C+VSCG GT+ R R    P   G K C G   E+  CNA  +CPV+  W  +  W SCS TCGGG Q   R       G G  C+G   E+R C+ +PC   G  T +    S+  +  +  + +       PLY  +  +   T        +          +   S   T          E N    D  P   P ++  T N     I  V    W  + TW+ CS+PCG G Q R+R+   P  N G++C G   Q++ CN H+C
Sbjct:  212 VDGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKG-KECDGPKDETESCNA-FSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHG-GETCIGHSEESRKCNEHPCPVDGVWT-AWADWSDCSVTCANGTQWRNRTCVGPLYGGMNCTGDETQEKICVAASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFFDGDPCEGPTEERLTCNTFNCPIDGV----WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYN-GSDCLGDWNQTKTCNTHSC 485          

HSP 2 Score: 102.449 bits (254), Expect = 3.272e-18
Identity = 138/532 (25.94%), Postives = 204/532 (38.35%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTT---------STTTTTTTTNTPIDNEV----NAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNK------ISDSYNGR---FAYEREGREQLYIXFFKSQADSLNLW-----VIGPEL--------GQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKD--DDPTLAVECFNGETTTTTNSN--FGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            +VD  W  +  W+ C+VSCG G+  R+R   QP   GG++C G ++E + CN    CPV+  ++ +G WS CS TCG G Q+  R+      G G+ C G+  +T+ C+T PC   G      + S+ SD   S    F        +Y T                +  T    P+S              T   T    T + N          K     D+D S+ N+    I  V    W+ +  W  CS+ CG G Q R R+   P   GG  C G   ++R CN H C            V  N +   D S+  G   +      I   Y G+    A+E       +      +  S   W       G  +        G F  G    G E    +   +       GVWKD       ++ C  G  T   + N  F      +     S    TQ       P  V+ E++ + +W  C  +CG G Q R+ R    P +GG NC G+ ++T++CN ++C
Sbjct:  486 AVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQP-LFGGENCTGPSVELQDCNT-FPCPVDGVFTAWGDWSVCSLTCGNGTQSRNRSCEGPYFG-GKNCTGDWGQTKDCNTFPCPVDGFWL---NWSNWSDCSKSCGRGF--------MYRTRVCEDPKYGGSECVGVSNETMPCNPSSCPVDGLWLSWGGWGTCNVTCGGGTRLRNRTCDGPYFGGKSCEGEDEDSSSCNDFPCPIDGV----WNPWSIWDNCSESCGGGNQSRYRTCDGPYY-GGMNCSGNDTEARDCNTHHCPVDG--------VFGNWSQWYDCSVTCGGGVQWRNRSCIGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQ--PCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHGGANCTGPDESSQACNTQ-------PCPVDGEFTDWTDWGKCDVTCGGGSQLRS-RACNGPFHGGQNCSGAWEETQTCNSQNC 980          

HSP 3 Score: 93.2041 bits (230), Expect = 2.328e-15
Identity = 134/541 (24.77%), Postives = 197/541 (36.41%), Query Frame = 0

Query:   36 TSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKI-LILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDV--------NCPLVNVIGATY------------ANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEP-------------WEGKWE-----EGVRVECVGGSK------------------KSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAAL---KDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            T S +   +   +R      M+     +   WS +SSW +CS +C  G + R R         GG  C     E + C+   CP     V  +         C +    G  Y             NC G Y+ T +  P   E PV      N ++ W+   +    G R   +T    +  G + D P              +G W+     E   V C GG +                   S  S   ++    VD +W+++  WS CSV+C  GTQ R R  + P   GG +C GD  + + C A A+CPVN  W  + +W SC+ TCGGG +  RR         G PC G   E  +C+T  C   G   E+  + ++            +   + P Y           T T  T + +        +  TS   +    +          L   ++   P  +L   N     +  V    ++ +  WSVCS  CG G Q RNRS   P   GG  C G   Q++ CN   C
Sbjct:   73 TGSESFLHQCLRVRADQCSVMAGVQCMVRGAWSLWSSWGNCSVTCENGTRTRTRVCNHPPPNFGGRECPDTDIEYKPCTMIMCP-----VDGIWREWSTWSECSVSCANGTKYRTRSCLGPFHEGQNCSGDYRETTDCFP--KECPV--DGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKGKECDGPKDETESCNAFSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHGGETCIGHSEESRKCNEHPCPVDGVWTAWADWSDCSVTCANGTQWRNRTCVGP-LYGGMNCTGDETQEKICVA-ASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFF-DGDPCEGPTEERLTCNTFNCPIDGV-WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYNGSDCLGDWNQTKTCNTHSCAVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGV----FTAWGDWSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNTFPC 595          

HSP 4 Score: 88.5817 bits (218), Expect = 6.716e-14
Identity = 85/297 (28.62%), Postives = 121/297 (40.74%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP-----HFVGDVPDVNCPLVNVIGATYANCDGRYKVTNN------KVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +D  + ++S W DCS +C GGVQ+R R   I    GG  C G  +ES  C+T  CP        G+    N      I     +CDG +   NN           + +P         +  W    L    G +  L+          D + P+ G         C G      SS + +     VD  ++ +  W +C V+CG G+Q R R    P  +GG++C G   E++ CN+   CP +  W  +  W  C+ TCGGG Q   R+      G G  C G   E R C  NPC
Sbjct:  762 VDGVFGNWSQWYDCSVTCGGGVQWRNRSC-IGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLR----------DCNGPFHGG------ANCTG---PDESSQACNTQPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGP-FHGGQNCSGAWEETQTCNS-QNCPEDGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRYG-GAECAGAGEEQRDCGKNPC 1035          

HSP 5 Score: 79.7221 bits (195), Expect = 3.030e-11
Identity = 80/299 (26.76%), Postives = 115/299 (38.46%), Query Frame = 0

Query:  227 SASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA-KTESRHSSSNSDIDNSISSSFSTIPTSSPLY--------TTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            S+S +D    +D +W+ +  W  CS SCG G Q R R    P   GG +C G+  E+R CN    CPV+  + N+ QW  CS TCGGG Q   R+      G G+ C G   E+ +C+T+PC   G  K+     + N      I           P +           +    T                  + +      T     N P     N        PD+     N     +    D E++D+  W  C   CG G Q R+R+   P  +GG  C G   +++ CN   C
Sbjct:  697 SSSCNDFPCPIDGVWNPWSIWDNCSESCGGGNQSRYRTCDGPYY-GGMNCSGNDTEARDCNT-HHCPVDGVFGNWSQWYDCSVTCGGGVQWRNRSCIGPFYG-GKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDC--DGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHGGANCTG-----PDESSQACNTQPCPV----DGEFTDWTDWGKCDVTCGGGSQLRSRACNGP-FHGGQNCSGAWEETQTCNSQNC 980          

HSP 6 Score: 74.7146 bits (182), Expect = 1.023e-9
Identity = 89/316 (28.16%), Postives = 122/316 (38.61%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIG--ATYANCDGRYKVTNNKVPWASER---PVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWE--GKWEEGVRVECVGGSKKSPSSASTSDDTGS------------------VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            ID  W+ +S W +CS+SC GG Q R R        GG  C G   E+R C+T              CP+  V G  + + +C          V W +     P Y             GG   S    E     +H        D  W+  G+W+    V C GG +K           G+                  VD +W  +  W  CS+SCG G Q R R    P  +GG +C G    S+ CN    CPV+ E++++  W  C  TCGGG Q   R       G G+ C G   ET++C++  C   G 
Sbjct:  707 IDGVWNPWSIWDNCSESCGGGNQSRYR-TCDGPYYGGMNCSGNDTEARDCNTHH------------CPVDGVFGNWSQWYDCS---VTCGGGVQWRNRSCIGPFY-------------GGKDCSGAFEESSTCNTH----PCPIDGKWKSWGEWDT-CNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGP-FHGGANCTGPDESSQACNT-QPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGPFHG-GQNCSGAWEETQTCNSQNCPEDGV 985          

HSP 7 Score: 65.0846 bits (157), Expect = 8.730e-7
Identity = 42/117 (35.90%), Postives = 56/117 (47.86%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGS-GRPCLGEKAETRSCSTNPCSRFGA 353
            D +W ++  W  C+V+CG G Q R R    P   GG  C G   E R C     CP+  +W ++  WS CSATCG G +   R+    + G    PC G    T  C+T PC  + A
Sbjct:  983 DGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRY-GGAECAGAGEEQRDCGKNP-CPIPGDWMSWSGWSRCSATCGTGVRWRTRDCNETSYGDLTAPCEGTSNGTEDCNTFPCRPYAA 1097          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|762092677|ref|XP_011428772.1| (PREDICTED: SCO-spondin-like isoform X2 [Crassostrea gigas])

HSP 1 Score: 115.161 bits (287), Expect = 4.748e-22
Identity = 88/283 (31.10%), Postives = 123/283 (43.46%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD-QPI--PDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            VD +W+ + QWS C+VSCG GT+ R R    P   G K C G   E+  CNA  +CPV+  W  +  W SCS TCGGG Q   R       G G  C+G   E+R C+ +PC   G  T +    S+  +  +  + +       PLY  +  +   T        +          +   S   T          E N    D  P   P ++  T N     I  V    W  + TW+ CS+PCG G Q R+R+   P  N G++C G   Q++ CN H+C
Sbjct:  212 VDGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKG-KECDGPKDETESCNA-FSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHG-GETCIGHSEESRKCNEHPCPVDGVWT-AWADWSDCSVTCANGTQWRNRTCVGPLYGGMNCTGDETQEKICVAASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFFDGDPCEGPTEERLTCNTFNCPIDGV----WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYN-GSDCLGDWNQTKTCNTHSC 485          

HSP 2 Score: 100.523 bits (249), Expect = 1.520e-17
Identity = 178/717 (24.83%), Postives = 263/717 (36.68%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWA--SERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEG------KWEE-----GVRVE-----CVG---GSKKSPSSASTSDDTGS----VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNK------ISDSYNGR---FAYEREGREQLYIXFFKSQADSLNLW-----VIGPEL--------GQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKD--DDPTLAVECFNGETTTTTNSN--FGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            +D  ++++ +WS CS SC G ++ R R   I    GG  C G R +S VC         GD P   CP+           DG + + ++   W   SE      T++ RY   N        G R     GS  H+   D   P +G       W E     G  ++     C G     K+   + S S +  +    VD ++  +  W+ CS+SCG+G Q R R+ + P   GG  C G   ++R CN    CPV+  W ++G W  C  TCGGG QT  R       G G PC G   +T+ C+T PC   G                              L+ +     T   T    T   + T   P     +             ++E +++  D P P D +                 W+ +  W  CS+ CG G Q R R+   P   GG  C G   ++R CN H C            V  N +   D S+  G   +      I   Y G+    A+E       +      +  S   W       G  +        G F  G    G E    +   +       GVWKD       ++ C  G  T   + N  F      +     S    TQ       P  V+ E++ + +W  C  +CG G Q R+ R    P +GG NC G+ ++T++CN ++C
Sbjct:  707 VDGLFTEWLTWSHCSVSCGGSLRNRTRSC-IGPFFGGKDCDGPRNDSEVC---------GDTP---CPV-----------DGEWSLWSS---WGGCSETCGKGQTQRYRYCTEN------KFGGRN-CSGGSLQHQDCNDFPCPIDGVFNPWSNWTECSKTCGTGIQSRNRTCDGPYHNGKECMGNYSDSQECNTHHCPVDGVFMEWSTWTDCSLSCGSGKQSRSRICVGPFY-GGSGCDGSFQDNRTCND-HHCPVDGVWDDWGPWKDCDVTCGGGNQTRERTCTGPFYG-GAPCNGSWNDTQECNTFPCPVDG------------------------------LWLSWGGWGTCNVTCGGGTRLRNRTCDGPYFGGKSCEG----------EDEDSSSCNDFPCPIDGV-----------------WNPWSIWDNCSESCGGGNQSRYRTCDGPYY-GGMNCSGNDTEARDCNTHHCPVDG--------VFGNWSQWYDCSVTCGGGVQWRNRSCIGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQ--PCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHGGANCTGPDESSQACNTQ-------PCPVDGEFTDWTDWGKCDVTCGGGSQLRS-RACNGPFHGGQNCSGAWEETQTCNSQNC 1310          

HSP 3 Score: 93.2041 bits (230), Expect = 2.449e-15
Identity = 134/541 (24.77%), Postives = 197/541 (36.41%), Query Frame = 0

Query:   36 TSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKI-LILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDV--------NCPLVNVIGATY------------ANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEP-------------WEGKWE-----EGVRVECVGGSK------------------KSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAAL---KDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            T S +   +   +R      M+     +   WS +SSW +CS +C  G + R R         GG  C     E + C+   CP     V  +         C +    G  Y             NC G Y+ T +  P   E PV      N ++ W+   +    G R   +T    +  G + D P              +G W+     E   V C GG +                   S  S   ++    VD +W+++  WS CSV+C  GTQ R R  + P   GG +C GD  + + C A A+CPVN  W  + +W SC+ TCGGG +  RR         G PC G   E  +C+T  C   G   E+  + ++            +   + P Y           T T  T + +        +  TS   +    +          L   ++   P  +L   N     +  V    ++ +  WSVCS  CG G Q RNRS   P   GG  C G   Q++ CN   C
Sbjct:   73 TGSESFLHQCLRVRADQCSVMAGVQCMVRGAWSLWSSWGNCSVTCENGTRTRTRVCNHPPPNFGGRECPDTDIEYKPCTMIMCP-----VDGIWREWSTWSECSVSCANGTKYRTRSCLGPFHEGQNCSGDYRETTDCFP--KECPV--DGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKGKECDGPKDETESCNAFSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHGGETCIGHSEESRKCNEHPCPVDGVWTAWADWSDCSVTCANGTQWRNRTCVGP-LYGGMNCTGDETQEKICVA-ASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFF-DGDPCEGPTEERLTCNTFNCPIDGV-WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYNGSDCLGDWNQTKTCNTHSCAVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGV----FTAWGDWSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNTFPC 595          

HSP 4 Score: 88.5817 bits (218), Expect = 7.262e-14
Identity = 85/297 (28.62%), Postives = 121/297 (40.74%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP-----HFVGDVPDVNCPLVNVIGATYANCDGRYKVTNN------KVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +D  + ++S W DCS +C GGVQ+R R   I    GG  C G  +ES  C+T  CP        G+    N      I     +CDG +   NN           + +P         +  W    L    G +  L+          D + P+ G         C G      SS + +     VD  ++ +  W +C V+CG G+Q R R    P  +GG++C G   E++ CN+   CP +  W  +  W  C+ TCGGG Q   R+      G G  C G   E R C  NPC
Sbjct: 1092 VDGVFGNWSQWYDCSVTCGGGVQWRNRSC-IGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLR----------DCNGPFHGG------ANCTG---PDESSQACNTQPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGP-FHGGQNCSGAWEETQTCNS-QNCPEDGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRYG-GAECAGAGEEQRDCGKNPC 1365          

HSP 5 Score: 84.7297 bits (208), Expect = 9.399e-13
Identity = 128/512 (25.00%), Postives = 180/512 (35.16%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE---------SRHSSSNSDIDNSISSSFSTIPTSSP------LYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDP--TLAVECFNGETT---TTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            VD  W ++  WS CS SCG G   R R+   P   GG  C G + E+  CN  ++CPV+  W+ +  WS C  TCG G Q   R       G G  C G + E + C T  C   G  TE         S   S  +   + I   F       P         T  P     +  ++    + T     T      T       N    +  +    D P P D +                 ++ +  W+ CSK CG G+Q RNR+   P  N G EC G    S+ CN H C            V    +   D SL  G+               ++ R ++ +                   G F  G   DG     ++T  +  +    GVW D  P     V C  G  T   T T   +G           S   T + +      +G+   W  +G W  C+ +CG G + R  R    P  GG +C G ++D+ SCN   C
Sbjct:  597 VDGFWLNWSNWSDCSKSCGRGFMYRTRVCEDPKY-GGSECVGVSNETMPCNP-SSCPVDGMWNTWQAWSDCFGTCGVGVQYRNRTCDGPFYG-GNNCTGPENENQECFTKECPVDGLFTEWLTWSHCSVSCGGSLRNRTRSCIGPFFGGKDCDGPRNDSEVCGDTPCPVDGEWSLWSSWGGCSETCGKGQTQRYRYCTENKFGGRNCSGGSLQHQDCNDFPCPIDGV-----------------FNPWSNWTECSKTCGTGIQSRNRTCDGPYHN-GKECMGNYSDSQECNTHHCPVDG--------VFMEWSTWTDCSLSCGS--------------GKQSRSRICV-------------------GPFYGGSGCDGSFQ-DNRTCND-HHCPVDGVWDDWGPWKDCDVTCGGGNQTRERTCTGPFYGGAP-----CNGSWNDTQECNTFPCPVDGL---WLSWGGWGTCNVTCGGGTRLR-NRTCDGPYFGGKSCEGEDEDSSSCNDFPC 1035          

HSP 6 Score: 83.5741 bits (205), Expect = 2.133e-12
Identity = 79/281 (28.11%), Postives = 112/281 (39.86%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            +VD  W  +  W+ C+VSCG G+  R+R   QP   GG++C G ++E + CN    CPV+  ++ +G WS CS TCG G Q+  R+      G G+ C G+  +T+ C+T PC   G      + S+ SD   S    F        +Y T                +  T    P+S               P+D   N                                 +  WS C   CGVGVQYRNR+   P   GGN C G   +++ C    C
Sbjct:  486 AVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQP-LFGGENCTGPSVELQDCNT-FPCPVDGVFTAWGDWSVCSLTCGNGTQSRNRSCEGPYFG-GKNCTGDWGQTKDCNTFPCPVDGFWL---NWSNWSDCSKSCGRGF--------MYRTRVCEDPKYGGSECVGVSNETMPCNPSS--------------CPVDGMWNT--------------------------------WQAWSDCFGTCGVGVQYRNRTCDGPFY-GGNNCTGPENENQECFTKEC 705          

HSP 7 Score: 81.2629 bits (199), Expect = 1.237e-11
Identity = 122/478 (25.52%), Postives = 177/478 (37.03%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGA--TYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEE-----GVRVECVGGSK----------------KSPSSASTSDDT--GSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLY---TTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            W  +S W  C+ +C GG + R R+       G  PC G  +E   C+T             NCP+  V  A  T+ +C  R   T  +V           +R     F+N       +G     KT +  H   +D      G W E        V C GGS                   PS      +T    VD +++++G WS CS++CG GTQ R R    P   GGK+C GD  +++ CN    CPV+  W N+  WS CS +CG G     R       G G  C+G   ET  C+ + C   G    +  + S+      +   +       P Y       P +      T         T   T +  + +   +    T          KD   P +D     ++   +    D EWS + +W  CS+ CG G   R R     ++ GG  C G + Q + CN   C
Sbjct:  381 WKPWSEWDSCNVTCGGGEKARRRECNGPFFDG-DPCEGPTEERLTCNT------------FNCPIDGVWEAWTTWTSCS-RPCGTGEQV----------RSRNCAGPFYNGSDC---LGDWNQTKTCNT-HSCAVD------GYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGVFTAWGDWSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNT-FPCPVDGFWLNWSNWSDCSKSCGRGFMYRTRVCEDPKYG-GSECVGVSNETMPCNPSSCPVDGMWN-TWQAWSDCFGTCGVGVQYRNRTCDGPFYGGNNCTGPENENQECFTKECPVDGLFTEWLTWSHCSVSCGGSLRNRTRSCIGPFFGGKDCDGPRNDSEVCGDTPCPV----DGEWSLWSSWGGCSETCGKGQTQRYRYCTE-NKFGGRNCSGGSLQHQDCNDFPC 815          

HSP 8 Score: 74.7146 bits (182), Expect = 9.996e-10
Identity = 89/316 (28.16%), Postives = 122/316 (38.61%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIG--ATYANCDGRYKVTNNKVPWASER---PVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWE--GKWEEGVRVECVGGSKKSPSSASTSDDTGS------------------VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            ID  W+ +S W +CS+SC GG Q R R        GG  C G   E+R C+T              CP+  V G  + + +C          V W +     P Y             GG   S    E     +H        D  W+  G+W+    V C GG +K           G+                  VD +W  +  W  CS+SCG G Q R R    P  +GG +C G    S+ CN    CPV+ E++++  W  C  TCGGG Q   R       G G+ C G   ET++C++  C   G 
Sbjct: 1037 IDGVWNPWSIWDNCSESCGGGNQSRYR-TCDGPYYGGMNCSGNDTEARDCNTHH------------CPVDGVFGNWSQWYDCS---VTCGGGVQWRNRSCIGPFY-------------GGKDCSGAFEESSTCNTH----PCPIDGKWKSWGEWDT-CNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGP-FHGGANCTGPDESSQACNT-QPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGPFHG-GQNCSGAWEETQTCNSQNCPEDGV 1315          

HSP 9 Score: 65.0846 bits (157), Expect = 9.736e-7
Identity = 42/117 (35.90%), Postives = 56/117 (47.86%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGS-GRPCLGEKAETRSCSTNPCSRFGA 353
            D +W ++  W  C+V+CG G Q R R    P   GG  C G   E R C     CP+  +W ++  WS CSATCG G +   R+    + G    PC G    T  C+T PC  + A
Sbjct: 1313 DGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRY-GGAECAGAGEEQRDCGKNP-CPIPGDWMSWSGWSRCSATCGTGVRWRTRDCNETSYGDLTAPCEGTSNGTEDCNTFPCRPYAA 1427          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|762092675|ref|XP_011428771.1| (PREDICTED: SCO-spondin-like isoform X1 [Crassostrea gigas])

HSP 1 Score: 115.161 bits (287), Expect = 4.829e-22
Identity = 88/283 (31.10%), Postives = 123/283 (43.46%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD-QPI--PDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            VD +W+ + QWS C+VSCG GT+ R R    P   G K C G   E+  CNA  +CPV+  W  +  W SCS TCGGG Q   R       G G  C+G   E+R C+ +PC   G  T +    S+  +  +  + +       PLY  +  +   T        +          +   S   T          E N    D  P   P ++  T N     I  V    W  + TW+ CS+PCG G Q R+R+   P  N G++C G   Q++ CN H+C
Sbjct:  212 VDGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKG-KECDGPKDETESCNA-FSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHG-GETCIGHSEESRKCNEHPCPVDGVWT-AWADWSDCSVTCANGTQWRNRTCVGPLYGGMNCTGDETQEKICVAASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFFDGDPCEGPTEERLTCNTFNCPIDGV----WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYN-GSDCLGDWNQTKTCNTHSC 485          

HSP 2 Score: 100.523 bits (249), Expect = 1.520e-17
Identity = 178/717 (24.83%), Postives = 263/717 (36.68%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWA--SERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEG------KWEE-----GVRVE-----CVG---GSKKSPSSASTSDDTGS----VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNK------ISDSYNGR---FAYEREGREQLYIXFFKSQADSLNLW-----VIGPEL--------GQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKD--DDPTLAVECFNGETTTTTNSN--FGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            +D  ++++ +WS CS SC G ++ R R   I    GG  C G R +S VC         GD P   CP+           DG + + ++   W   SE      T++ RY   N        G R     GS  H+   D   P +G       W E     G  ++     C G     K+   + S S +  +    VD ++  +  W+ CS+SCG+G Q R R+ + P   GG  C G   ++R CN    CPV+  W ++G W  C  TCGGG QT  R       G G PC G   +T+ C+T PC   G                              L+ +     T   T    T   + T   P     +             ++E +++  D P P D +                 W+ +  W  CS+ CG G Q R R+   P   GG  C G   ++R CN H C            V  N +   D S+  G   +      I   Y G+    A+E       +      +  S   W       G  +        G F  G    G E    +   +       GVWKD       ++ C  G  T   + N  F      +     S    TQ       P  V+ E++ + +W  C  +CG G Q R+ R    P +GG NC G+ ++T++CN ++C
Sbjct:  707 VDGLFTEWLTWSHCSVSCGGSLRNRTRSC-IGPFFGGKDCDGPRNDSEVC---------GDTP---CPV-----------DGEWSLWSS---WGGCSETCGKGQTQRYRYCTEN------KFGGRN-CSGGSLQHQDCNDFPCPIDGVFNPWSNWTECSKTCGTGIQSRNRTCDGPYHNGKECMGNYSDSQECNTHHCPVDGVFMEWSTWTDCSLSCGSGKQSRSRICVGPFY-GGSGCDGSFQDNRTCND-HHCPVDGVWDDWGPWKDCDVTCGGGNQTRERTCTGPFYG-GAPCNGSWNDTQECNTFPCPVDG------------------------------LWLSWGGWGTCNVTCGGGTRLRNRTCDGPYFGGKSCEG----------EDEDSSSCNDFPCPIDGV-----------------WNPWSIWDNCSESCGGGNQSRYRTCDGPYY-GGMNCSGNDTEARDCNTHHCPVDG--------VFGNWSQWYDCSVTCGGGVQWRNRSCIGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQ--PCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHGGANCTGPDESSQACNTQ-------PCPVDGEFTDWTDWGKCDVTCGGGSQLRS-RACNGPFHGGQNCSGAWEETQTCNSQNC 1310          

HSP 3 Score: 93.5893 bits (231), Expect = 2.232e-15
Identity = 134/541 (24.77%), Postives = 197/541 (36.41%), Query Frame = 0

Query:   36 TSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKI-LILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDV--------NCPLVNVIGATY------------ANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEP-------------WEGKWE-----EGVRVECVGGSK------------------KSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAAL---KDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            T S +   +   +R      M+     +   WS +SSW +CS +C  G + R R         GG  C     E + C+   CP     V  +         C +    G  Y             NC G Y+ T +  P   E PV      N ++ W+   +    G R   +T    +  G + D P              +G W+     E   V C GG +                   S  S   ++    VD +W+++  WS CSV+C  GTQ R R  + P   GG +C GD  + + C A A+CPVN  W  + +W SC+ TCGGG +  RR         G PC G   E  +C+T  C   G   E+  + ++            +   + P Y           T T  T + +        +  TS   +    +          L   ++   P  +L   N     +  V    ++ +  WSVCS  CG G Q RNRS   P   GG  C G   Q++ CN   C
Sbjct:   73 TGSESFLHQCLRVRADQCSVMAGVQCMVRGAWSLWSSWGNCSVTCENGTRTRTRVCNHPPPNFGGRECPDTDIEYKPCTMIMCP-----VDGIWREWSTWSECSVSCANGTKYRTRSCLGPFHEGQNCSGDYRETTDCFP--KECPV--DGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKGKECDGPKDETESCNAFSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHGGETCIGHSEESRKCNEHPCPVDGVWTAWADWSDCSVTCANGTQWRNRTCVGP-LYGGMNCTGDETQEKICVA-ASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFF-DGDPCEGPTEERLTCNTFNCPIDGV-WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYNGSDCLGDWNQTKTCNTHSCAVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGV----FTAWGDWSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNTFPC 595          

HSP 4 Score: 88.5817 bits (218), Expect = 7.449e-14
Identity = 85/297 (28.62%), Postives = 121/297 (40.74%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP-----HFVGDVPDVNCPLVNVIGATYANCDGRYKVTNN------KVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +D  + ++S W DCS +C GGVQ+R R   I    GG  C G  +ES  C+T  CP        G+    N      I     +CDG +   NN           + +P         +  W    L    G +  L+          D + P+ G         C G      SS + +     VD  ++ +  W +C V+CG G+Q R R    P  +GG++C G   E++ CN+   CP +  W  +  W  C+ TCGGG Q   R+      G G  C G   E R C  NPC
Sbjct: 1092 VDGVFGNWSQWYDCSVTCGGGVQWRNRSC-IGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLR----------DCNGPFHGG------ANCTG---PDESSQACNTQPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGP-FHGGQNCSGAWEETQTCNS-QNCPEDGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRYG-GAECAGAGEEQRDCGKNPC 1365          

HSP 5 Score: 84.7297 bits (208), Expect = 1.006e-12
Identity = 128/512 (25.00%), Postives = 180/512 (35.16%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE---------SRHSSSNSDIDNSISSSFSTIPTSSP------LYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDP--TLAVECFNGETT---TTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            VD  W ++  WS CS SCG G   R R+   P   GG  C G + E+  CN  ++CPV+  W+ +  WS C  TCG G Q   R       G G  C G + E + C T  C   G  TE         S   S  +   + I   F       P         T  P     +  ++    + T     T      T       N    +  +    D P P D +                 ++ +  W+ CSK CG G+Q RNR+   P  N G EC G    S+ CN H C            V    +   D SL  G+               ++ R ++ +                   G F  G   DG     ++T  +  +    GVW D  P     V C  G  T   T T   +G           S   T + +      +G+   W  +G W  C+ +CG G + R  R    P  GG +C G ++D+ SCN   C
Sbjct:  597 VDGFWLNWSNWSDCSKSCGRGFMYRTRVCEDPKY-GGSECVGVSNETMPCNP-SSCPVDGMWNTWQAWSDCFGTCGVGVQYRNRTCDGPFYG-GNNCTGPENENQECFTKECPVDGLFTEWLTWSHCSVSCGGSLRNRTRSCIGPFFGGKDCDGPRNDSEVCGDTPCPVDGEWSLWSSWGGCSETCGKGQTQRYRYCTENKFGGRNCSGGSLQHQDCNDFPCPIDGV-----------------FNPWSNWTECSKTCGTGIQSRNRTCDGPYHN-GKECMGNYSDSQECNTHHCPVDG--------VFMEWSTWTDCSLSCGS--------------GKQSRSRICV-------------------GPFYGGSGCDGSFQ-DNRTCND-HHCPVDGVWDDWGPWKDCDVTCGGGNQTRERTCTGPFYGGAP-----CNGSWNDTQECNTFPCPVDGL---WLSWGGWGTCNVTCGGGTRLR-NRTCDGPYFGGKSCEGEDEDSSSCNDFPC 1035          

HSP 6 Score: 83.5741 bits (205), Expect = 2.225e-12
Identity = 79/281 (28.11%), Postives = 112/281 (39.86%), Query Frame = 0

Query:  236 SVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            +VD  W  +  W+ C+VSCG G+  R+R   QP   GG++C G ++E + CN    CPV+  ++ +G WS CS TCG G Q+  R+      G G+ C G+  +T+ C+T PC   G      + S+ SD   S    F        +Y T                +  T    P+S               P+D   N                                 +  WS C   CGVGVQYRNR+   P   GGN C G   +++ C    C
Sbjct:  486 AVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQP-LFGGENCTGPSVELQDCNT-FPCPVDGVFTAWGDWSVCSLTCGNGTQSRNRSCEGPYFG-GKNCTGDWGQTKDCNTFPCPVDGFWL---NWSNWSDCSKSCGRGF--------MYRTRVCEDPKYGGSECVGVSNETMPCNPSS--------------CPVDGMWNT--------------------------------WQAWSDCFGTCGVGVQYRNRTCDGPFY-GGNNCTGPENENQECFTKEC 705          

HSP 7 Score: 80.8777 bits (198), Expect = 1.334e-11
Identity = 122/478 (25.52%), Postives = 177/478 (37.03%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGA--TYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEE-----GVRVECVGGSK----------------KSPSSASTSDDT--GSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLY---TTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            W  +S W  C+ +C GG + R R+       G  PC G  +E   C+T             NCP+  V  A  T+ +C  R   T  +V           +R     F+N       +G     KT +  H   +D      G W E        V C GGS                   PS      +T    VD +++++G WS CS++CG GTQ R R    P   GGK+C GD  +++ CN    CPV+  W N+  WS CS +CG G     R       G G  C+G   ET  C+ + C   G    +  + S+      +   +       P Y       P +      T         T   T +  + +   +    T          KD   P +D     ++   +    D EWS + +W  CS+ CG G   R R     ++ GG  C G + Q + CN   C
Sbjct:  381 WKPWSEWDSCNVTCGGGEKARRRECNGPFFDG-DPCEGPTEERLTCNT------------FNCPIDGVWEAWTTWTSCS-RPCGTGEQV----------RSRNCAGPFYNGSDC---LGDWNQTKTCNT-HSCAVD------GYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGVFTAWGDWSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNT-FPCPVDGFWLNWSNWSDCSKSCGRGFMYRTRVCEDPKYG-GSECVGVSNETMPCNPSSCPVDGMWN-TWQAWSDCFGTCGVGVQYRNRTCDGPFYGGNNCTGPENENQECFTKECPVDGLFTEWLTWSHCSVSCGGSLRNRTRSCIGPFFGGKDCDGPRNDSEVCGDTPCPV----DGEWSLWSSWGGCSETCGKGQTQRYRYCTE-NKFGGRNCSGGSLQHQDCNDFPC 815          

HSP 8 Score: 74.7146 bits (182), Expect = 1.069e-9
Identity = 89/316 (28.16%), Postives = 122/316 (38.61%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIG--ATYANCDGRYKVTNNKVPWASER---PVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWE--GKWEEGVRVECVGGSKKSPSSASTSDDTGS------------------VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA 353
            ID  W+ +S W +CS+SC GG Q R R        GG  C G   E+R C+T              CP+  V G  + + +C          V W +     P Y             GG   S    E     +H        D  W+  G+W+    V C GG +K           G+                  VD +W  +  W  CS+SCG G Q R R    P  +GG +C G    S+ CN    CPV+ E++++  W  C  TCGGG Q   R       G G+ C G   ET++C++  C   G 
Sbjct: 1037 IDGVWNPWSIWDNCSESCGGGNQSRYR-TCDGPYYGGMNCSGNDTEARDCNTHH------------CPVDGVFGNWSQWYDCS---VTCGGGVQWRNRSCIGPFY-------------GGKDCSGAFEESSTCNTH----PCPIDGKWKSWGEWDT-CNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGP-FHGGANCTGPDESSQACNT-QPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGPFHG-GQNCSGAWEETQTCNSQNCPEDGV 1315          

BLAST of EMLSAG00000000316 vs. nr
Match: gi|405954292|gb|EKC21775.1| (SCO-spondin [Crassostrea gigas])

HSP 1 Score: 115.161 bits (287), Expect = 4.978e-22
Identity = 88/283 (31.10%), Postives = 123/283 (43.46%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKD-QPI--PDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            VD +W+ + QWS C+VSCG GT+ R R    P   G K C G   E+  CNA  +CPV+  W  +  W SCS TCGGG Q   R       G G  C+G   E+R C+ +PC   G  T +    S+  +  +  + +       PLY  +  +   T        +          +   S   T          E N    D  P   P ++  T N     I  V    W  + TW+ CS+PCG G Q R+R+   P  N G++C G   Q++ CN H+C
Sbjct:  201 VDGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKG-KECDGPKDETESCNA-FSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHG-GETCIGHSEESRKCNEHPCPVDGVWT-AWADWSDCSVTCANGTQWRNRTCVGPLYGGMNCTGDETQEKICVAASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFFDGDPCEGPTEERLTCNTFNCPIDGV----WEAWTTWTSCSRPCGTGEQVRSRNCAGPFYN-GSDCLGDWNQTKTCNTHSC 474          

HSP 2 Score: 93.9745 bits (232), Expect = 1.534e-15
Identity = 136/541 (25.14%), Postives = 196/541 (36.23%), Query Frame = 0

Query:   36 TSSTTSTKKMSSLRERLAIFMSQFDGRIDCKWSDFSSWSDCSQSCNGGVQFRERKI-LILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDV--------NCPLVNVIGATY------------ANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEP-------------WEGKWE-----EGVRVECVGGSK------------------KSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAAL---KDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            T S +   +   +R      M+     +   WS +SSW +CS +C  G + R R         GG  C     E + C+   CP     V  +         C +    G  Y             NC G Y+ T +  P   E PV      N ++ W+   +    G R   +T    +  G + D P              +G W+     E   V C GG +                   S  S   ++    VD +W+++  WS CSV+C  GTQ R R  + P   GG +C GD  + + C A A+CPVN  W  + +W SC+ TCGGG +  RR         G PC G   E  +C+T  C   G   E+  + ++            +   + P Y           T T  T + +        +  TS   +    +          L   ++   P  +L   N     +  V    W D   WSVCS  CG G Q RNRS   P   GG  C G   Q++ CN   C
Sbjct:   62 TGSESFLHQCLRVRADQCSVMAGVQCMVRGAWSLWSSWGNCSVTCENGTRTRTRVCNHPPPNFGGRECPDTDIEYKPCTMIMCP-----VDGIWREWSTWSECSVSCANGTKYRTRSCLGPFHEGQNCSGDYRETTDCFP--KECPV--DGVWNGWMQWSECTVSCGYGTRNRSRTCKGPYYKGKECDGPKDETESCNAFSCPVDGSWKTWNGWESCSVTCGGGIQNRTRECNLPLHGGETCIGHSEESRKCNEHPCPVDGVWTAWADWSDCSVTCANGTQWRNRTCVGP-LYGGMNCTGDETQEKICVA-ASCPVNGVWKPWSEWDSCNVTCGGGEKARRRECNGPFF-DGDPCEGPTEERLTCNTFNCPIDGVW-EAWTTWTSCSRPCGTGEQVRSRNCAGPFYNGSDCLGDWNQTKTCNTHSCAVDGYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGVFT-AWGD---WSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNTFPC 584          

HSP 3 Score: 92.0485 bits (227), Expect = 5.885e-15
Identity = 124/484 (25.62%), Postives = 186/484 (38.43%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCD---GRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSK------------KSPSSASTSDDTGS------VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCE---------WSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            ID  ++ +S+W++CS++C  G+Q R R         G  C G   +S+ C+T  CP             V +  +T+ +C    G  K + +++      P Y             GG G   G  +  +T +  H  G+  D    G W++   V C GG++             +P + S +D          VD LW S+G W  C+V+CG GT+ R R    P   GGKSC G+  +S  CN    CP++  W+ +  W +CS +CGGG Q+  R       G G  C G   E R C+T+ C   G        + +   D S++        +       +     +     ++T    T   P      S      T N      +   ++D   P       NN        Q+C          W D+  W  CS  CG G+Q R R    P   G N C G    S+ CN   C
Sbjct:  806 IDGVFNPWSNWTECSKTCGTGIQSRNR-TCDGPYHNGKECMGNYSDSQECNTHHCPV----------DGVFMEWSTWTDCSLSCGSGKQSRSRI---CVGPFY-------------GGSG-CDGSFQDNRTCNDHHFDGVWDDW---GPWKD-CDVTCGGGNQTRERTCTGPFYGGAPCNGSWNDTQECNTFPCPVDGLWLSWGGWGTCNVTCGGGTRLRNRTCDGPYF-GGKSCEGEDEDSSSCND-FPCPIDGVWNPWSIWDNCSESCGGGNQSRYRTCDGPYYG-GMNCSGNDTEARDCNTHHCPVDGV-----FGNWSQWYDCSVTCGGGVQWRNRSCIGPFYGGKDCSGAFEESSTC--NTHPCPIDGKWKS-WGEWDTCNVTCGGGIQKRVRDCDGP---FHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHGGAN-CTGPDESSQACNTQPC 1242          

HSP 4 Score: 89.3521 bits (220), Expect = 3.747e-14
Identity = 123/479 (25.68%), Postives = 177/479 (36.95%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPD--------VNCPLVN------VIGATY--ANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGA-KTESRHSSSNSDIDNSISSSFSTIPTSSPLY--------TTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            +D  + ++S+W+DCS SC  G Q R R I +    GGS C G  +++R C+     HF G   D        V C   N        G  Y  A C+G +  T        E   +       ++ W     GW       +  G          D P+ G    G   E      +   S+S +D    +D +W+ +  W  CS SCG G Q R R    P   GG +C G+  E+R CN    CPV+  + N+ QW  CS TCGGG Q   R+      G G+ C G   E+ +C+T+PC   G  K+     + N      I           P +           +    T                  + +      T     N P     N        PD+     N     +    D E++D+  W  C   CG G Q R+R+   P  +GG  C G   +++ CN   C
Sbjct:  861 VDGVFMEWSTWTDCSLSCGSGKQSRSR-ICVGPFYGGSGCDGSFQDNRTCNDH---HFDGVWDDWGPWKDCDVTCGGGNQTRERTCTGPFYGGAPCNGSWNDTQ-------ECNTFPCPVDGLWLSWG----GWGTCN---VTCGGGTRLRNRTCDGPYFG----GKSCE-----GEDEDSSSCNDFPCPIDGVWNPWSIWDNCSESCGGGNQSRYRTCDGPYY-GGMNCSGNDTEARDCNT-HHCPVDGVFGNWSQWYDCSVTCGGGVQWRNRSCIGPFYG-GKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDC--DGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHGGANCTG-----PDESSQACNTQPCPV----DGEFTDWTDWGKCDVTCGGGSQLRSRACNGP-FHGGQNCSGAWEETQTCNSQNC 1297          

HSP 5 Score: 88.9669 bits (219), Expect = 4.551e-14
Identity = 85/297 (28.62%), Postives = 121/297 (40.74%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP-----HFVGDVPDVNCPLVNVIGATYANCDGRYKVTNN------KVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +D  + ++S W DCS +C GGVQ+R R   I    GG  C G  +ES  C+T  CP        G+    N      I     +CDG +   NN           + +P         +  W    L    G +  L+          D + P+ G         C G      SS + +     VD  ++ +  W +C V+CG G+Q R R    P  +GG++C G   E++ CN+   CP +  W  +  W  C+ TCGGG Q   R+      G G  C G   E R C  NPC
Sbjct: 1079 VDGVFGNWSQWYDCSVTCGGGVQWRNRS-CIGPFYGGKDCSGAFEESSTCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGPFHGGNNCTGPEDSYQECNTQPCPVDGVWKDWAGWGECSLSCGGGIQTRLR----------DCNGPFHGG------ANCTG---PDESSQACNTQPCPVDGEFTDWTDWGKCDVTCGGGSQLRSRACNGP-FHGGQNCSGAWEETQTCNS-QNCPEDGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRYG-GAECAGAGEEQRDCGKNPC 1352          

HSP 6 Score: 82.0333 bits (201), Expect = 7.066e-12
Identity = 130/510 (25.49%), Postives = 176/510 (34.51%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE---------SRHSSSNSDIDNSISSSFSTIPTSSP------LYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETT---TTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSC 728
            VD  W ++  WS CS SCG G   R R+   P   GG  C G + E+  CN  ++CPV+  W+ +  WS C  TCG G Q   R       G G  C G + E + C T  C   G  TE         S   S  +   + I   F       P         T  P     +  ++    + T     T      T       N    +  +    D P P D +                 WS+   W+ CSK CG G+Q RNR+   P  N G EC G    S+ CN H C            V    +   D SL  G+               ++ R ++ +  F                G    G   D +    H     W      G WKD D    V C  G  T   T T   +G           S   T + +      +G+   W   G W  C+ +CG G + R  R    P  GG +C G ++D+ SCN   C
Sbjct:  586 VDGFWLNWSNWSDCSKSCGRGFMYRTRVCEDPKY-GGSECVGVSNETMPCNP-SSCPVDGMWNTWQAWSDCFGTCGVGVQYRNRTCDGPFYG-GNNCTGPENENQECFTKECPVDGLFTEWLTWSHCSVSCGGSLRNRTRSCIGPFFGGKDCDGPRNDSEVCGDTPCPVDGEWSLWSSWGGCSETCGKGQTQRYRYCTENKFGGRNCSGGSLQHQDCNDFPCPIDGVFN--------------PWSN---WTECSKTCGTGIQSRNRTCDGPYHN-GKECMGNYSDSQECNTHHCPVDG--------VFMEWSTWTDCSLSCGS--------------GKQSRSRICVGPFYG--------------GSGCDGSFQDNRTCNDHHFDGVWD---DWGPWKDCD----VTCGGGNQTRERTCTGPFYGGAP-----CNGSWNDTQECNTFPCPVDGLWLSW---GGWGTCNVTCGGGTRLR-NRTCDGPYFGGKSCEGEDEDSSSCNDFPC 1022          

HSP 7 Score: 81.6481 bits (200), Expect = 8.438e-12
Identity = 122/478 (25.52%), Postives = 177/478 (37.03%), Query Frame = 0

Query:   67 WSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGA--TYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEE-----GVRVECVGGSK----------------KSPSSASTSDDT--GSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLY---TTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            W  +S W  C+ +C GG + R R+       G  PC G  +E   C+T             NCP+  V  A  T+ +C  R   T  +V           +R     F+N       +G     KT +  H   +D      G W E        V C GGS                   PS      +T    VD +++++G WS CS++CG GTQ R R    P   GGK+C GD  +++ CN    CPV+  W N+  WS CS +CG G     R       G G  C+G   ET  C+ + C   G    +  + S+      +   +       P Y       P +      T         T   T +  + +   +    T          KD   P +D     ++   +    D EWS + +W  CS+ CG G   R R     ++ GG  C G + Q + CN   C
Sbjct:  370 WKPWSEWDSCNVTCGGGEKARRRECNGPFFDG-DPCEGPTEERLTCNT------------FNCPIDGVWEAWTTWTSCS-RPCGTGEQV----------RSRNCAGPFYNGSDC---LGDWNQTKTCNT-HSCAVD------GYWHEWSNWTSCNVSCGGGSIWRRRRCEQPLFGGENCTGPSVELQDCNTFPCPVDGVFTAWGDWSVCSLTCGNGTQSRNRSCEGPYF-GGKNCTGDWGQTKDCNT-FPCPVDGFWLNWSNWSDCSKSCGRGFMYRTRVCEDPKYG-GSECVGVSNETMPCNPSSCPVDGMW-NTWQAWSDCFGTCGVGVQYRNRTCDGPFYGGNNCTGPENENQECFTKECPVDGLFTEWLTWSHCSVSCGGSLRNRTRSCIGPFFGGKDCDGPRNDSEVCGDTPCPV----DGEWSLWSSWGGCSETCGKGQTQRYRYCTE-NKFGGRNCSGGSLQHQDCNDFPC 804          

HSP 8 Score: 80.4925 bits (197), Expect = 2.192e-11
Identity = 113/452 (25.00%), Postives = 165/452 (36.50%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCP-------HFVGDVPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASE-RPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTT----NTPIDN------EVNAALKDQ--------PIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNR 488
            +D  W  +  W  C+ +C GG + R R        GG  C GE ++S  C+   CP         + D    +C   N   + Y  CDG Y    N     +E R    H      +F N     WS      +  G            P+ G        +C G  ++S   ++ +     +D  W S+G+W  C+V+CG G Q+R R    P  +GG +C G     ++CN    CPV+  W ++  W  CS +CGGG QT  R+      G G  C G    +++C+T PC   G  T+        D+     S   +   + P +     S     T T  +          T +     T T        N   D       E   A ++Q        PIP D                   W  +  WS CS  CG GV++R R
Sbjct:  969 VDGLWLSWGGWGTCNVTCGGGTRLRNR-TCDGPYFGGKSCEGEDEDSSSCNDFPCPIDGVWNPWSIWDNCSESCGGGNQ--SRYRTCDGPYYGGMNCSGNDTEARDCNTHHCPVDGVFGN-----WSQWYDCSVTCGGGVQWRNRSCIGPFYGG------KDCSGAFEES---STCNTHPCPIDGKWKSWGEWDTCNVTCGGGIQKRVRDCDGP-FHGGNNCTGPEDSYQECNT-QPCPVDGVWKDWAGWGECSLSCGGGIQTRLRDCNGPFHG-GANCTGPDESSQACNTQPCPVDGEFTD-WTDWGKCDVTCGGGSQLRSRACNGPFHGGQNCSGAWEETQTCNSQNCPEDGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRYGGAECAGAGEEQRDCGKNPCPIPGD-------------------WMSWSGWSRCSATCGTGVRWRTR 1380          

HSP 9 Score: 65.4698 bits (158), Expect = 6.877e-7
Identity = 42/117 (35.90%), Postives = 56/117 (47.86%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGS-GRPCLGEKAETRSCSTNPCSRFGA 353
            D +W ++  W  C+V+CG G Q R R    P   GG  C G   E R C     CP+  +W ++  WS CSATCG G +   R+    + G    PC G    T  C+T PC  + A
Sbjct: 1300 DGVWMTWSLWGDCTVTCGGGLQYRNRSCDGPRY-GGAECAGAGEEQRDCGKNP-CPIPGDWMSWSGWSRCSATCGTGVRWRTRDCNETSYGDLTAPCEGTSNGTEDCNTFPCRPYAA 1414          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold249_size238305-snap-gene-1.12 (protein:Tk01320 transcript:maker-scaffold249_size238305-snap-gene-1.12-mRNA-1 annotation:"PREDICTED: hemicentin-1-like")

HSP 1 Score: 286.189 bits (731), Expect = 1.993e-83
Identity = 158/333 (47.45%), Postives = 199/333 (59.76%), Query Frame = 0

Query:  124 VPDVNCPLVNVIGATYANCDGRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKKSPSSAST--------------SDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCS------RFGAKTESRHSSSNSDIDNSISSS---FSTIP---TSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPI 430
            VPDV CP +NV+GATYA CDG Y+VTN  V WA +RPVY+H  K+RY+FWNAGGLGWSIGKR+YL TGSHWHRSGLD+DEPW+G WE GV V CV  + +  S+ +               + D G V+CLWSSYG WS CSVSCG+G QQRKRM+LQ + NGG  CRGDAI+S+ C+A  TCPV+CEWSN+G WS CSA+CGGG ++  R +   A   GR C+G   ETR C+   CS           T +   +   D+  + + S   FST+P   T  P ++ LF  +    T    T    T            T    +  N P+
Sbjct:  260 VPDVICPTINVLGATYALCDGEYRVTNEVVDWAPDRPVYRHESKDRYVFWNAGGLGWSIGKRDYLNTGSHWHRSGLDSDEPWQGPWERGVMVNCVEDNPRDLSTQAKPEILNNRAQQRPNQAQDKGPVNCLWSSYGAWSSCSVSCGSGVQQRKRMVLQEAENGGTPCRGDAIQSQACSA-RTCPVDCEWSNYGDWSRCSASCGGGVESRERTVLTEARNGGRRCIGRNRETRICNAESCSTTTEEPNTTTTTTTTTVTREEDVPTTTTPSRTEFSTVPSTRTIQPGFSPLFDLTDQQLTAHQVTDQQLTDRQLTEGLGGAKTANGQSMRNGPL 591          

HSP 2 Score: 71.633 bits (174), Expect = 1.070e-12
Identity = 30/56 (53.57%), Postives = 40/56 (71.43%), Query Frame = 0

Query:  674 VNCEWSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSNCVGSNKDTRSCNVKSCN 729
            V+CEWS YG+WS CS SCG G++ R R V    +NGG  C+G N++TR CN +SC+
Sbjct:  443 VDCEWSNYGDWSRCSASCGGGVESRERTVLTEARNGGRRCIGRNRETRICNAESCS 498          

HSP 3 Score: 65.855 bits (159), Expect = 5.543e-11
Identity = 27/55 (49.09%), Postives = 38/55 (69.09%), Query Frame = 0

Query:   63 IDCKWSDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQC 117
            +DC+WS++  WS CS SC GGV+ RER +L  AR GG  C G  +E+R+C+ + C
Sbjct:  443 VDCEWSNYGDWSRCSASCGGGVESRERTVLTEARNGGRRCIGRNRETRICNAESC 497          

HSP 4 Score: 65.0846 bits (157), Expect = 9.666e-11
Identity = 28/54 (51.85%), Postives = 37/54 (68.52%), Query Frame = 0

Query:  463 DCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            DCEWS+Y  WS CS  CG GV+ R R+++  +RNGG  C GR  ++RICN  +C
Sbjct:  444 DCEWSNYGDWSRCSASCGGGVESRERTVLTEARNGGRRCIGRNRETRICNAESC 497          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold249_size238305-snap-gene-1.13 (protein:Tk01321 transcript:maker-scaffold249_size238305-snap-gene-1.13-mRNA-1 annotation:"PREDICTED: collectin-12-like")

HSP 1 Score: 144.05 bits (362), Expect = 2.749e-35
Identity = 91/235 (38.72%), Postives = 121/235 (51.49%), Query Frame = 0

Query: 1077 LLPTTGCNIRDISEPQSSSIEACLCTTDFCNDDLGRSPGGDATSQIQERQTTRSRFTDTTNSPTRSRFSETTKSSNFFERTTKASTFFDQKSRASTPGFRPDFNDLPDKFEFNRNDNLRKTSCPRGYTLTPTGCYQISDRRMGWIEAKKLCE--SENGNLLAIDNEEERQAILELISESSPRTRFEFWLGGNDIEIENRWXWXGF-GNTILXPDFGWLDRPVPSVEENCLTWSIT 1308
            LLP  GC +RDISEP +S +EACLCTT+ CN   G    G A   IQ             ++P R+  ++   + N        +T             RP  N             ++ + CP  +   PTGCY  +  RMGWIEAKK CE  +   NL+AI+   ER A++  IS S  R+R+EFW  GNDI+ E+ W W G  G  +  P+FGW+D P+ S EENCLTWS+T
Sbjct:  349 LLPLNGCQVRDISEPGASGVEACLCTTNLCNSRDGAV--GAAVGPIQ-------------SAPIRNPPTQPPANINPVAVPQTVTTR------------RPSQN------------QIQGSQCPPKFKQLPTGCYFFAQERMGWIEAKKECEFMTPGTNLVAIETPGERDALMSEIS-SFGRSRYEFWTAGNDIDREDEWVWSGSRGRKV--PEFGWIDLPITSAEENCLTWSLT 541          

HSP 2 Score: 127.102 bits (318), Expect = 6.798e-30
Identity = 60/104 (57.69%), Postives = 75/104 (72.12%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSGNPPCEEGNAGKTE---YCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNANG 984
            R+LCHQCGSLFSGS NP C   +A   E   YCK GE+CL+Y+W+KS  ETSIIREC + SILLG I  PL+  R C  +DI E ++S+I AC+CDT++CN  G
Sbjct:   75 RVLCHQCGSLFSGSNNPDCTNFDASSEEFQGYCKPGESCLYYTWEKSVSETSIIRECITPSILLGPITNPLRASRTCDVRDISEFKSSSIMACICDTDKCNVGG 178          

HSP 3 Score: 106.686 bits (265), Expect = 1.854e-23
Identity = 67/169 (39.64%), Postives = 89/169 (52.66%), Query Frame = 0

Query:  738 PTTTTRRPVVKAKPIDPNRRVKCFACGSLFS-TDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVE--NDDSIMACICATDFCN----GYDGGSPVNLKXTTTPTPLRLPQSQTERPRILCHQCGSLFSGSGN 899
            P  T     V  +  +PNRRV C  CGSLFS ++ P+C  FD S+ + Q  C  GE+CL+Y++ KS  + +IIREC + SILLG I NP+  S  C  R +      SIMACIC TD CN         SP     +   TP   P+S  +   +L  +  S+  G GN
Sbjct:   56 PAPTQSAITVPPRNANPNRRVLCHQCGSLFSGSNNPDCTNFDASSEEFQGYCKPGESCLYYTWEKSVSETSIIRECITPSILLGPITNPLRASRTCDVRDISEFKSSSIMACICDTDKCNVGGTSVTANSPGGFPGSRDSTP-EEPKSSQKTTLVLTDEETSILGGLGN 223          

HSP 4 Score: 101.679 bits (252), Expect = 6.092e-22
Identity = 59/141 (41.84%), Postives = 79/141 (56.03%), Query Frame = 0

Query:  757 RVKCFACGSLFSTDAPECDKFDVSASQQQTTCARGEACLWYSYHKSAGDIAIIRECFSTSILLGSIENPIAPSHECVPRPVENDDS--IMACICATDFCNGYDGG----------SPVNLKXTTTPT---PLRLPQSQTER 882
            +++CF+CGSLF+ D  +CDKFD S      TC  GEACL YS+ KS G+ A  RECFSTSILLG I N + P + C  R +    +  + AC+C T+ CN  DG           +P+    T  P    P+ +PQ+ T R
Sbjct:  281 QLQCFSCGSLFNPDK-KCDKFDPSDPSLIQTCGPGEACLLYSWEKSTGERATARECFSTSILLGPINNQLLPLNGCQVRDISEPGASGVEACLCTTNLCNSRDGAVGAAVGPIQSAPIRNPPTQPPANINPVAVPQTVTTR 420          

HSP 5 Score: 77.7962 bits (190), Expect = 1.239e-14
Identity = 44/102 (43.14%), Postives = 57/102 (55.88%), Query Frame = 0

Query:  884 RILCHQCGSLFSGSGNPPCEE---GNAGKTEYCKEGEACLWYSWKKSNIETSIIRECFSKSILLGSIDKPLQLRRECVPQDIXETRASNIYACLCDTERCNA 982
            ++ C  CGSLF+      C++    +    + C  GEACL YSW+KS  E +  RECFS SILLG I+  L     C  +DI E  AS + ACLC T  CN+
Sbjct:  281 QLQCFSCGSLFNPDKK--CDKFDPSDPSLIQTCGPGEACLLYSWEKSTGERATARECFSTSILLGPINNQLLPLNGCQVRDISEPGASGVEACLCTTNLCNS 380          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold591_size129331-processed-gene-0.4 (protein:Tk08230 transcript:snap_masked-scaffold591_size129331-processed-gene-0.4-mRNA-1 annotation:"SCO-spondin")

HSP 1 Score: 78.9518 bits (193), Expect = 8.774e-15
Identity = 119/529 (22.50%), Postives = 195/529 (36.86%), Query Frame = 0

Query:  225 PSSASTSDDTGSVDC-LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRP-----CLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDC-EWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTCGSGADQCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLA----VEC-----FNGETTTTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCE--------WSQYGEWSPCSKSCGRGIQFRTRRVAV---TPKNGGSNC-VGSNKDTRSCNV 725
            P S   S+D  + DC  ++ + +W+ CS++CG+GT+ + R  + P       C G+      CN    CPV  EW+++GQ   C+ATCGGG +   R+    A+ +G       C G+  E R C+ + C  +   TE    S        + +    +P + P              I    T    T   PT  T     + +    T          +DQ   D +    N         Q C  W+++  W+ C+K C  G + R R  V  +R   ++CPG   +   CN   C +         +       +   +   G   ++ D  +    ++  G  +         AD+   +    E  +  A     G +T + +  Q  + A++     D    +     VEC     +N     +     G RQ+  R  T    T  +L       E   C+        WS + EWS CS  CG G +   R  +       NG S C  G  +++ +CN 
Sbjct: 1092 PGSNEESEDCNTQDCPYFTEWTEWTDCSLTCGSGTRSKVRECIVPRDGDANICNGETRTQESCNE-TPCPVWSEWTDWGQ---CTATCGGGTEKRIRDCLLAAARNGNGTNFYGCDGDTWEMRGCNEHNCPTWTEWTEFTDCSKTCGGGRRVKTRECNLPPNIPAPALALFCPGDEKVIEDCNT---GTCPKPTEWTEWGECSVSCGGGTRQKTRECVNFRDQ---DGNPCNENLVDEEPCNEQPCPAWTEWTEWTSCTKTCDKGRRKRARDCVLFTR---SDCPGEDEELEDCNPEPCPA---------LTPWGEWTQCTLTCGGGTQRRVRDCLSPISGFDSNGCMEPLEEIMVCSADACPAFTEWSEWTECSADC-GGGTKTHVRECTQNGEEAAQDTCLGDALEQVPCNDDVECPRWTEWNTWGDCSVTCGEGTRQR-GRECTRPVETNGKLLCNGEDLEVGPCDPSVPQCPMWSDWSEWSDCSDECGGGSRTHVRVCSAGNEVDANGKSPCGFGDFEESEACNA 1596          

HSP 2 Score: 77.0258 bits (188), Expect = 3.943e-14
Identity = 87/335 (25.97%), Postives = 134/335 (40.00%), Query Frame = 0

Query:  210 EEGVRVECVGGSKKSPSSASTSDDTGSVDC-LWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGA-TCPVNCEWSNFGQWSSCSATCGGGRQTXRR--NIRRRASGSGR-PC-LGEKAETRSCSTN-PCSRFGAKTESRHSSSNS--------DIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGN---------ECPGRAFQSRICNRHTCGSG 519
            EE  +  C+G + +       +DD   V+C  W+ +  W  CSV+CG GT+QR R   +P  TNG   C G+ +E   C+     CP+   WS++ +WS CS  CGGG +T  R  +       +G+ PC  G+  E+ +C+ +  C      +E    ++ S        +      SS  T     P    +F        I    T          S  +        +      + V+     Q  P+       +S+ +++      WS++  WS CSK CG G Q R+R   H +   GN          CPG   Q+R C    C  G
Sbjct: 1463 EEAAQDTCLGDALEQ---VPCNDD---VECPRWTEWNTWGDCSVTCGEGTRQRGRECTRPVETNGKLLCNGEDLEVGPCDPSVPQCPM---WSDWSEWSDCSDECGGGSRTHVRVCSAGNEVDANGKSPCGFGDFEESEACNADIKCPELALWSEWSEWNACSQSCGGGMKERARQCGSSDGTTGCPGPSRGIMF------CNIDPCPTDADWAEWGEWSQCSVPCGGGDRSRRRDCSSSVSTGYGPQTSPECPGDELEHSKCNVQACST--WSEWTMWSTCSKTCGKGAQRRSRDCQHTNLPRGNYPRLGLLASPCPGEPQQARSCILQECPLG 1780          

HSP 3 Score: 69.3218 bits (168), Expect = 8.311e-12
Identity = 111/493 (22.52%), Postives = 172/493 (34.89%), Query Frame = 0

Query:   68 SDFSSWSDCSQSCNGGVQFRERKILILARQGGSPCRGERKESRVCSTQQCPHFVGDVPDVNCPLVNVIGATYANCD-GRYKVTNNKVPWASERPVYKHTRKNRYIFWNAGGLGWSIGKREYLKTGSHWHRSGLDTDEPWEGKWEEGVRVECVGGSKK----SPSSASTSDDTGSVDCL-------------WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGK-SCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGR-PCLGEKAETRSC--STNPCSRF---------------GAKTESRHSSSNSDIDNSISS--SFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGN-----ECPGRAFQSRICNRHTC 516
            ++++ W +CS SC GG + + R+ +    Q G+PC     +   C+ Q CP +       +C            CD GR K           R     TR +              G+ E L+  +      L    PW G+W +   + C GG+++      S  S  D  G ++ L             ++ + +W+ CS  CG GT+   R   Q      + +C GDA+E   CN    CP    W+ +  W  CS TCG G +   R   R    +G+  C GE  E   C  S   C  +               G++T  R  S+ +++D +  S   F     S      +        +  +     S +             ++  TT  P  +         P P D                  +W+++  WS CS PCG G + R R        G       ECPG   +   CN   C
Sbjct: 1278 TEWTEWGECSVSCGGGTRQKTRECVNFRDQDGNPCNENLVDEEPCNEQPCPAWTEWTEWTSC---------TKTCDKGRRKRA---------RDCVLFTRSD------------CPGEDEELEDCNPEPCPALT---PW-GEWTQ-CTLTCGGGTQRRVRDCLSPISGFDSNGCMEPLEEIMVCSADACPAFTEWSEWTECSADCGGGTKTHVRECTQNGEEAAQDTCLGDALEQVPCNDDVECP---RWTEWNTWGDCSVTCGEGTRQRGRECTRPVETNGKLLCNGEDLEVGPCDPSVPQCPMWSDWSEWSDCSDECGGGSRTHVRVCSAGNEVDANGKSPCGFGDFEESEACNADIKCPELALWSEWSEWNACSQSCGGGMKERARQCGSSDGTTGCPGPSRGIMFCNIDPCPTDA-----------------DWAEWGEWSQCSVPCGGGDRSRRRDCSSSVSTGYGPQTSPECPGDELEHSKCNVQAC 1715          

HSP 4 Score: 68.5514 bits (166), Expect = 1.402e-11
Identity = 84/335 (25.07%), Postives = 121/335 (36.12%), Query Frame = 0

Query:  216 ECVGGSKKSPSSASTSDDTGSVDCL-------------WSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC------SRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTT------TTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDC---------EWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            ECVG +    SS+S  D+   ++ L             WS +G+W+ CS +CG G + R R    P T     C G   ES  CN    CP   EW+   +W+ CS TCG G ++  R       G    C GE     SC+  PC      + +G  T +    +   I + + ++       +   T  +     T  +         T T  T  T  S T        T   N P +  + A       P D+           K ++DC         EW++   W  CS  CG G + + R  V+     GN C         CN   C
Sbjct: 1018 ECVGPNANDVSSSSNGDENACMEPLEVIEACNEDTCPVWSVWGEWTICSKTCGGGERTRYRQCTDPVTGQEAFCPGSNEESEDCNT-QDCPYFTEWT---EWTDCSLTCGSGTRSKVRECIVPRDGDANICNGETRTQESCNETPCPVWSEWTDWGQCTATCGGGTEKRIRDCLLAA-----ARNGNGTNFYGCDGDTWEMRGCNEHNCPTWTEWTEFTDCSKTCGGGRRVKTRECNLPPN--IPAPALALFCPGDE-----------KVIEDCNTGTCPKPTEWTE---WGECSVSCGGGTRQKTRECVNFRDQDGNPCNENLVDEEPCNEQPC 1327          

HSP 5 Score: 63.5438 bits (153), Expect = 4.203e-10
Identity = 41/122 (33.61%), Postives = 61/122 (50.00%), Query Frame = 0

Query:  242 SSYGQWSRCSVSCGTGTQQRKRMILQP-------STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTE 356
            + +  WS+CS +CG G +Q+ R  + P       S+NG ++   + +E  +     TCPV   WS +G+W+ CS TCGGG +T  R      +G    C G   E+  C+T  C  F   TE
Sbjct:  996 TEWADWSQCSHTCGGGFRQKTRECVGPNANDVSSSSNGDENACMEPLEVIEACNEDTCPV---WSVWGEWTICSKTCGGGERTRYRQCTDPVTGQEAFCPGSNEESEDCNTQDCPYFTEWTE 1114          

HSP 6 Score: 62.3882 bits (150), Expect = 1.054e-9
Identity = 121/549 (22.04%), Postives = 196/549 (35.70%), Query Frame = 0

Query:  240 LWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIES----RQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCL----GEKAETRSCSTNP-CSRFGAKTESRHSSSNSDIDNSISSSFSTIPTSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNE------VNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPS--RNGGNEC-PGRAFQSRICNRH----------------TCGSGADQCCERVIVKANGNAKL---DQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETT---------TTTNSNFGNRQQISRRTTVSTTTTTQLSIIRNRPEGVNCE------WSQYGEWSPCSKSCGRGIQFRTRRVAVTPKNGGSN-CVGSNKDTRSCNVKSCNFFTTTT 735
            L++ + +WS CS +CG G Q+R+R    P  N    C G+  +S    R CN    CP    W+ +  WS CSA+CGGG+    R       G+ RP      GE  ET +C+ N  C   G  TE    S +    +   +    +P S+ + +   P+S          +        P  T  +  +  + T               N+ L  + + ++D  T N S           W+++  WS CS  CG G +   R    P+  +  G++C  G A +   CN                  TCG G     E+  ++  G  KL   D    L    K+                         +  +L  W    +      G        C+   A +   +S        +P   +E  N +T          T  +   G  ++   R      T  +     +  E  +C       ++++ EW+ CS +CG G + + R   V P++G +N C G  +   SCN   C  ++  T
Sbjct:  659 LFNEWSEWSPCSATCGGGGQERERNCYDP--NFRLKCDGEPRQSRFVVRACNVDP-CP---SWTQWSAWSQCSASCGGGQNARSREC-TLPDGAVRPNAECGEGESNETGNCNENVLCPVLGEWTEWSQCSVSCGGGDRSRTRTCGVPGSTQIASASTPASENPCQQPLFESEKCNDDRCPVYTPWSEWSQCSATCGGGSQRRNRQCVPANSRLFCEGVSEED-RTCNESPCP-------NWTEWGEWSECSFTCGGGTRQNTRQCELPNGEQVKGSQCGVGPATEEESCNTDQCPELGPWTEWGPCTVTCGGG-----EKTRMRECGLPKLLRDDNPCQLPLLEKME--------------------CHPEECPTLTEWADWSQCSHTCGGGFRQKTRECVGPNANDVSSSSNGDENACMEPLEVIEACNEDTCPVWSVWGEWTICSKTCGGGERTRYRQCTDPVTGQEAFCPGSNEESEDCNTQDCPYFTEWTEWTDCSLTCGSGTRSKVRECIV-PRDGDANICNGETRTQESCNETPCPVWSEWT 1166          

HSP 7 Score: 58.9214 bits (141), Expect = 1.157e-8
Identity = 74/297 (24.92%), Postives = 113/297 (38.05%), Query Frame = 0

Query:  242 SSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRR-----NIRRRASGSGRPCLGEKAETRSCSTNPCSRFGAKTESRHSSSNSDIDNSISSSFSTIP-----------------TSSPLYTTLFPSSTTTTTITTTTTTTSTTTTTPTSTTTTSTTTTTTTTNTPIDNEVNAALKDQPIPDDDLSTTNNSRSSIKRVQDCEWSDYDTWSVCSKPCGVGVQYRNRSIVHPSRNGGNECPGRAFQSRICNRHTC 516
            S + +WS C+ +C +G + R+R       N    C     E   CN    CP+  EWS   +WS CSATCGGG Q   R     N R +  G  R     +   R+C+ +PC  +   +     S++     +  S   T+P                 T +     L P     T  +  + +      + T T     +T   + +TP     +     QP+ + +    N+ R  +       ++ +  WS CS  CG G Q RNR  V    N    C G + + R CN   C
Sbjct:  609 SEWSEWSDCTKTCESGERSRER-TCDDQRNENNPCNARLTEMETCNT-QLCPLFNEWS---EWSPCSATCGGGGQERERNCYDPNFRLKCDGEPRQ---SRFVVRACNVDPCPSWTQWSAWSQCSASCGGGQNARSRECTLPDGAVRPNAECGEGESNETGNCNENVLCPVLGEWTEWSQCSVSCGGGDRSRTRTCGVPGSTQIASASTP----ASENPCQQPLFESE--KCNDDRCPV-------YTPWSEWSQCSATCGGGSQRRNRQCV--PANSRLFCEGVSEEDRTCNESPC 882          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold476_size161517-snap-gene-0.39 (protein:Tk11952 transcript:maker-scaffold476_size161517-snap-gene-0.39-mRNA-1 annotation:"collagen alpha-1 chain-like")

HSP 1 Score: 69.707 bits (169), Expect = 4.127e-12
Identity = 45/120 (37.50%), Postives = 63/120 (52.50%), Query Frame = 0

Query:  237 VDCLWSSYGQWSRCSVS---CGTGTQQRKRMILQPS-TNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSC---SATCGGGRQTXRR---NIRRRASGSGRPCLGEKAETRSCSTN 346
            VD +WS +G WS C      CG G Q+R R    P  ++GG SC GD +ES+ CN    CP+N   +++ +WSSC   + TCG G +T  R   N      G   P     +E+R+C T+
Sbjct:  325 VDGVWSGWGLWSECQFDADDCGNGMQERTRACNDPPPSHGGDSCAGDGVESQMCN--VPCPINGGLADWSEWSSCVHNTGTCGLGLETRTRTCTNPSPDFGGMSCPDADPNSESRTCFTD 442          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold835_size90571-snap-gene-0.11 (protein:Tk02833 transcript:maker-scaffold835_size90571-snap-gene-0.11-mRNA-1 annotation:"netrin receptor partial")

HSP 1 Score: 69.707 bits (169), Expect = 5.604e-12
Identity = 42/112 (37.50%), Postives = 57/112 (50.89%), Query Frame = 0

Query:  238 DCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRA-SGSGRPCLGEKAETRSCSTNPC 348
            D  WS +  WS CSV CG GTQ+R R    P + GG+ C G  ++ R C +     ++ +W+ +  WS CS  C    Q  RR     A S +GR C G   E+R+CS   C
Sbjct:  321 DGTWSMWASWSSCSVDCGKGTQKRTRSCKLPKS-GGQPCPGSPVQKRPCKSTCKGQIDGDWTQWSSWSPCSPEC---FQHRRRTCSNPAPSANGRYCDGLDLESRNCSDGFC 428          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold94_size379870-snap-gene-0.14 (protein:Tk09624 transcript:maker-scaffold94_size379870-snap-gene-0.14-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_321105")

HSP 1 Score: 62.3882 bits (150), Expect = 8.092e-10
Identity = 39/128 (30.47%), Postives = 64/128 (50.00%), Query Frame = 0

Query:  222 KKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCG-GGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC 348
            +++  +A   D+   VDCL +++  WS C+ SCG   +++ R I +  T+ G+SC       ++C    +C  +C    +GQWS CS +CG  G Q+  R +    +  G  C G + E R C    C
Sbjct:  793 ERARKAADNEDELRVVDCLVTAWSPWSDCNSSCGKAFRRKFRTIQRYPTDNGRSCPKKLERKQKCRL-PSCQQDCILGLWGQWSPCSKSCGEDGEQSRTREVIHSPNHQGDLC-GAEMEKRFCLLATC 918          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold84_size396325-snap-gene-2.37 (protein:Tk09689 transcript:maker-scaffold84_size396325-snap-gene-2.37-mRNA-1 annotation:"pan domain-containing protein")

HSP 1 Score: 56.225 bits (134), Expect = 2.430e-8
Identity = 34/117 (29.06%), Postives = 55/117 (47.01%), Query Frame = 0

Query:  522 QCCERVIVKANGNAKLDQSLYLGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGETTT 638
            +CC+ + + A G     Q   LG Y K  ++ +GR  Y++ G EQ ++ +  S      LW++G  +G  + GI N G   C      +W+Y      W ++D T+   C N   TT
Sbjct:  131 ECCQELRLDATGMGDFYQGDRLGLYRKYVETSSGRNVYQQVGGEQ-FLYYLDS-----GLWMVGDTVGNDLGGILNRGSAQCPEDLTGDWEYWGGFDEW-EEDWTMEATCQNSGGTT 240          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold327_size205035-snap-gene-1.9 (protein:Tk05413 transcript:maker-scaffold327_size205035-snap-gene-1.9-mRNA-1 annotation:"PREDICTED: spondin-1-like")

HSP 1 Score: 55.8398 bits (133), Expect = 8.821e-8
Identity = 36/123 (29.27%), Postives = 55/123 (44.72%), Query Frame = 0

Query:  247 WSRCSVSCGTGTQQRKRMILQPSTNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSCSATCGGGRQTXRRNIRRRASGSGRPCLGEKAETRSCSTNPC---SRFGAKTESRHSSSNSDI 366
            + RC   C +    R   +  PS  G    +  A+ S + +      ++C+ S + +WS CS +CG G QT  R I     G G+PC  +    + C   PC   SR+  +   RH  SN D+
Sbjct:  639 YKRCMSMCASQFGSRNWDVQNPSPFG----KLQALNSIKIDPNPRISIDCQMSAWSEWSECSKSCGIGWQTRTREIVLPPQGQGKPCPQKLERKKKCRKIPCPTDSRYWYRGSWRHMVSNDDL 757          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold444_size168727-snap-gene-0.41 (protein:Tk11375 transcript:maker-scaffold444_size168727-snap-gene-0.41-mRNA-1 annotation:"hypothetical protein CAPTEDRAFT_227712")

HSP 1 Score: 51.9878 bits (123), Expect = 6.830e-7
Identity = 32/94 (34.04%), Postives = 47/94 (50.00%), Query Frame = 0

Query:  543 LGAYNKISDSYNGRFAYEREGREQLYIXFFKSQADSLNLWVIGPELGQFIAGIRNDGQETCIHKTAQEWKYASRAGVWKDDDPTLAVECFNGET 636
            LG Y++  D ++ R AY    R +     F     +  LW++GPE+G+F  G+ N G   C     + WKYA  +G WK  D  L +EC   +T
Sbjct:    1 LGIYHREGD-FSDRPAYVHAQRTE---RLFYIDGRARGLWMVGPEIGRFSGGLANRGDTKCPENIKRPWKYADVSG-WK-SDAQLTIECHEQDT 88          

BLAST of EMLSAG00000000316 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold376_size191502-snap-gene-0.26 (protein:Tk10505 transcript:maker-scaffold376_size191502-snap-gene-0.26-mRNA-1 annotation:"hypothetical protein BRAFLDRAFT_67461")

HSP 1 Score: 51.9878 bits (123), Expect = 7.924e-7
Identity = 34/110 (30.91%), Postives = 50/110 (45.45%), Query Frame = 0

Query:  216 ECVGGSKKSPSSASTSDDTGSVDCLWSSYGQWSRCSVSCGTGTQQRKRMILQP-STNGGKSCRGDAIESRQCNAGATCPVNCEWSNFGQWSSC---SATCGGGRQTXRRN 321
            E V  ++ SP S + +     V+ +WS +  W  C+  C    + R R    P   + G  C G   E R C + A CP    W+ +G WS C   S +CG G  + +RN
Sbjct:   70 EAVNATRASPRSIAQAPLKLRVNGVWSEWSAWDSCASDCSKVKEYRNRACDNPRPKHAGSLCDGATQEERDCTSEAACP--GAWAIWGVWSPCHSASGSCGLGTGSKQRN 177