BLAST of EMLSAG00000000629 vs. GO
Match: - (symbol:CELE_Y48C3A.20 "Protein Y48C3A.20" species:6239 "Caenorhabditis elegans" [GO:0001522 "pseudouridine synthesis" evidence=IEA] [GO:0003723 "RNA binding" evidence=IEA] [GO:0009451 "RNA modification" evidence=IEA] [GO:0009982 "pseudouridine synthase activity" evidence=IEA] InterPro:IPR020103 GO:GO:0003723 GO:GO:0009982 GO:GO:0001522 SUPFAM:SSF55120 eggNOG:COG1258 GeneTree:ENSGT00390000007529 KO:K07583 EMBL:AL117203 RefSeq:NP_496819.1 UniGene:Cel.14029 ProteinModelPortal:Q9U287 SMR:Q9U287 STRING:6239.Y48C3A.20 PaxDb:Q9U287 EnsemblMetazoa:Y48C3A.20.1 EnsemblMetazoa:Y48C3A.20.2 GeneID:174977 KEGG:cel:CELE_Y48C3A.20 UCSC:Y48C3A.20 CTD:174977 WormBase:Y48C3A.20 HOGENOM:HOG000021933 InParanoid:Q9U287 OMA:CAYCYSI NextBio:886264 Uniprot:Q9U287)

HSP 1 Score: 134.035 bits (336), Expect = 5.753e-33
Identity = 92/247 (37.25%), Postives = 136/247 (55.06%), Query Frame = 0

Query:  226 LPQTPWI--IDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKT-----IFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVV--VIP-SDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGC---DIDIEALDVQAINLDWPP 457
            LPQ+PW    D  R+   S+ E + +  KK F   D++F +SGRED+D RMLG+GRPF  E  N R T     I   E L++V+ AIN   +DV++ SL+ + +   + L  GE+ K K Y +    ++P SD + T      K+P   + + QKTPVRV+ RR+   R+R +  + F           D  F   L+ +    +KEFVHGDF RT PSL  ++     ++DI  LDV+ ++L+WPP
Sbjct:  224 LPQSPWSEDKDAPREPGNSVSEKVCDLLKKQFGASDSRFVTSGREDIDVRMLGTGRPFVVELRNCRTTAPVRGIKYLETLKTVQEAINVQ-KDVKVVSLTRVPRDVSEKLCVGEEDKRKQYCAYCYSILPISDSQFT--AAQSKIP---LKIVQKTPVRVMKRRALLDRERWIYSMNFLKL-------DDHHFEVRLETQAGTYIKEFVHGDFGRTRPSLAELLDVEHGEVDILELDVEKVDLEWPP 457          

BLAST of EMLSAG00000000629 vs. GO
Match: - (symbol:pus10 "tRNA pseudouridine synthase Pus10" species:243232 "Methanocaldococcus jannaschii DSM 2661" [GO:0009982 "pseudouridine synthase activity" evidence=IDA] [GO:0031119 "tRNA pseudouridine synthesis" evidence=IDA] HAMAP:MF_01893 InterPro:IPR004114 InterPro:IPR005912 InterPro:IPR020103 Pfam:PF02926 PROSITE:PS51165 GO:GO:0000049 EMBL:L77117 GO:GO:0009982 SUPFAM:SSF55120 GO:GO:0031119 eggNOG:COG1258 KO:K07583 PIR:A64305 RefSeq:NP_247004.1 ProteinModelPortal:Q60346 STRING:243232.MJ0041 EnsemblBacteria:AAB98022 GeneID:1450879 KEGG:mja:MJ_0041 OMA:PQTRWPC ProtClustDB:PRK14554 TIGRFAMs:TIGR01213 Uniprot:Q60346)

HSP 1 Score: 96.2857 bits (238), Expect = 3.653e-20
Identity = 80/242 (33.06%), Postives = 112/242 (46.28%), Query Frame = 0

Query:  225 NLPQTPWIIDGVR------------KLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRF--IFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAIN 452
             +PQT W     R            K   S+EEII +PF +  K  DAKF+ +GRED+D RMLG GRPF  E   P+       +L  +   IN   + V + +L   ++ D    K    +  KTYR++V   SD     ++   +      T+ QKTP RVLHRR++  R R V  +K        S   D  F  I        +KE + GD  RT PS+ SI+  +   + LDV  I+
Sbjct:  219 GIPQTRWPCRKCRGKGCELCNYTGKKYPISVEEIIAKPFLEATKGVDAKFHGAGREDIDVRMLGDGRPFVLEIKEPK---IRKIDLNKIAEEINKDGR-VEVLNLEFGVRKDKVIFKNTPHR--KTYRALVEC-SDKITDEELKLLEKELENRTIYQKTPKRVLHRRADLERIRKVYKVK-------TSKVDDNHFEMIIYCDGGLYIKELISGDDGRTNPSVSSILNKNCICKELDVLKIH 446          

BLAST of EMLSAG00000000629 vs. GO
Match: - (symbol:pus10 "tRNA pseudouridine synthase Pus10" species:186497 "Pyrococcus furiosus DSM 3638" [GO:0009982 "pseudouridine synthase activity" evidence=IDA] [GO:0031119 "tRNA pseudouridine synthesis" evidence=IDA] HAMAP:MF_01893 InterPro:IPR004114 InterPro:IPR005912 InterPro:IPR020103 Pfam:PF02926 PROSITE:PS51165 SMART:SM00981 GO:GO:0000049 EMBL:AE009950 GO:GO:0009982 SUPFAM:SSF55120 GO:GO:0031119 eggNOG:COG1258 KO:K07583 OMA:PQTRWPC ProtClustDB:PRK14554 TIGRFAMs:TIGR01213 RefSeq:NP_578868.1 ProteinModelPortal:Q8U1R6 STRING:186497.PF1139 PRIDE:Q8U1R6 EnsemblBacteria:AAL81263 GeneID:1469008 KEGG:pfu:PF1139 HOGENOM:HOG000227928 BioCyc:MetaCyc:MONOMER-16703 Uniprot:Q8U1R6)

HSP 1 Score: 89.7373 bits (221), Expect = 4.112e-18
Identity = 75/231 (32.47%), Postives = 107/231 (46.32%), Query Frame = 0

Query:  225 NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVV-----VIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDV 448
             +PQTP           SI  II   FKK F      F  +GREDVD RMLG+GRPF  E   PRK   +   L+ +E  IN S    ++  L+L   +  +  +    +  K Y ++V     +   +VE  +  L          + Q+TP RVL+ R++ +R R V D+K E          D+ F   L  +    +KE + GD  RTTPS+  I+G +   E LDV
Sbjct:  172 GIPQTP-----APGFKESIATIICRAFKKHF-HGKCIFKGAGREDVDVRMLGNGRPFVVEIKRPRKRKVN---LKDIEEEINQSG---KVEVLNLRFITPEEAERILTTRHRKVYEAIVYVKDGITKEEVEKVVKSL------KNAEIKQRTPRRVLNSRADLVRVRKVYDVKGELI-------DDKHFKLRLVTDGGLYIKELISGDRGRTTPSVSEILGKEAWCEILDV 377          

BLAST of EMLSAG00000000629 vs. GO
Match: - (symbol:PF14_0023 "Putative uncharacterized protein" species:36329 "Plasmodium falciparum 3D7" [GO:0008150 "biological_process" evidence=ND] InterPro:IPR020103 GO:GO:0003723 EMBL:AE014187 GO:GO:0009982 GO:GO:0001522 SUPFAM:SSF55120 RefSeq:XP_001348196.1 ProteinModelPortal:Q8IM68 SMR:Q8IM68 STRING:5833.PF14_0023-1 EnsemblProtists:PF14_0023:mRNA GeneID:811587 KEGG:pfa:PF14_0023 EuPathDB:PlasmoDB:PF3D7_1402100 HOGENOM:HOG000281398 ProtClustDB:CLSZ2432776 Uniprot:Q8IM68)

HSP 1 Score: 60.8474 bits (146), Expect = 1.819e-8
Identity = 27/62 (43.55%), Postives = 34/62 (54.84%), Query Frame = 0

Query:  222 FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAF 283
            +   + QT W+I+ +     SIEE I   F  IF F  A F  SGRED D RM+  GRPF +
Sbjct:  431 YNKEISQTKWLINNISNSVLSIEECISNIFNNIFSFSTATFIGSGREDKDARMMNIGRPFVY 492          

HSP 2 Score: 60.4622 bits (145), Expect = 2.486e-8
Identity = 49/157 (31.21%), Postives = 81/157 (51.59%), Query Frame = 0

Query:  311 DVRINSLSLLLKSDL--KTLKEGEDQKTKTYRSVVVIPSDVEM-TLDVLNGKLPASK-------VTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEP-TVKEFVHGDFKRTTPSLKSIIGCD--IDIEALDVQAINLD 454
            ++++N+LS      L  K +K GE++K K Y+ ++   S +   T+  +N ++   +       + + QKTP+RVLHRRS   R+R +  +K     K  S       ++ L +    +KEFV+GD  RT P+LK   G D  ++I  LDV  +  D
Sbjct:  730 EIKLNNLSFSTNYPLVKKLMKYGEERK-KEYKCIIYHSSKMNKDTIQKINDQISNHEPQTLTYVLKIIQKTPIRVLHRRSLINRERKIFQIKLIYLHKHFS------LLYLLAESGLYIKEFVNGDKGRTIPNLKQFFGDDTYVNILNLDVATLIYD 879          

BLAST of EMLSAG00000000629 vs. GO
Match: - (symbol:PF14_0023 "hypothetical protein, conserved" species:5833 "Plasmodium falciparum" [GO:0008150 "biological_process" evidence=ND] InterPro:IPR020103 GO:GO:0003723 EMBL:AE014187 GO:GO:0009982 GO:GO:0001522 SUPFAM:SSF55120 RefSeq:XP_001348196.1 ProteinModelPortal:Q8IM68 SMR:Q8IM68 STRING:5833.PF14_0023-1 EnsemblProtists:PF14_0023:mRNA GeneID:811587 KEGG:pfa:PF14_0023 EuPathDB:PlasmoDB:PF3D7_1402100 HOGENOM:HOG000281398 ProtClustDB:CLSZ2432776 Uniprot:Q8IM68)

HSP 1 Score: 60.8474 bits (146), Expect = 1.819e-8
Identity = 27/62 (43.55%), Postives = 34/62 (54.84%), Query Frame = 0

Query:  222 FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAF 283
            +   + QT W+I+ +     SIEE I   F  IF F  A F  SGRED D RM+  GRPF +
Sbjct:  431 YNKEISQTKWLINNISNSVLSIEECISNIFNNIFSFSTATFIGSGREDKDARMMNIGRPFVY 492          

HSP 2 Score: 60.4622 bits (145), Expect = 2.486e-8
Identity = 49/157 (31.21%), Postives = 81/157 (51.59%), Query Frame = 0

Query:  311 DVRINSLSLLLKSDL--KTLKEGEDQKTKTYRSVVVIPSDVEM-TLDVLNGKLPASK-------VTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEP-TVKEFVHGDFKRTTPSLKSIIGCD--IDIEALDVQAINLD 454
            ++++N+LS      L  K +K GE++K K Y+ ++   S +   T+  +N ++   +       + + QKTP+RVLHRRS   R+R +  +K     K  S       ++ L +    +KEFV+GD  RT P+LK   G D  ++I  LDV  +  D
Sbjct:  730 EIKLNNLSFSTNYPLVKKLMKYGEERK-KEYKCIIYHSSKMNKDTIQKINDQISNHEPQTLTYVLKIIQKTPIRVLHRRSLINRERKIFQIKLIYLHKHFS------LLYLLAESGLYIKEFVNGDKGRTIPNLKQFFGDDTYVNILNLDVATLIYD 879          

BLAST of EMLSAG00000000629 vs. C. finmarchicus
Match: gi|592824268|gb|GAXK01130300.1| (TSA: Calanus finmarchicus comp70521_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 310.071 bits (793), Expect = 6.705e-98
Identity = 212/489 (43.35%), Postives = 302/489 (61.76%), Query Frame = 0

Query:    4 SYKESVFQALEEAQDICFRCKLRYSGLRE--VYKYRTSEAQNGSEG-KISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFXXXXXXXXXXXXRERSLTIFLTEKI-----EKFRSDDIIPIKQVWKWLFPTELKAEK--KYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPS--------LFESN-------------LPQTPWIIDGVRKLDTSXXXXXXXXXXXXXXXXXXXXYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINXXXXXXXXXXXXXXEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKL-PASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGC-DIDIEALDVQAINLDWPPGS 459
            S K+ + + L +A   C +C  R+ G +   VY     E +   EG K +K N C  C+G LQD +M+P L++V  SI  S YD+  FS+++SLPI LSLR+ SL + L  ++          +DI+PIKQVWK+++P  +  E   ++  G  +DFFAEL++++ ED  E++ +  +CKEEY  R K  + Y+MG+ +RQG+EKSL  V+++ F ++Y VPP +PS        +F S+             LPQTPW I+GVR  +TSIEE+I E   +  K  + KF +SGREDVD RMLGSGRPFAFE VNPRKT F+HEEL  +E  INS    V++NSL ++ K D+K LKEGE++K K Y ++ V     E   D++  KL   + +TL Q+TP+RVLHRRSNA+R++ V ++K E   +E    +  + + S      VKEFVHGDF RT P++ S++GC   DI ALDV+ + L WPPG 
Sbjct:  100 SDKDLIIRTLADA-GACGKCCHRFVGEKGSMVYLGIHQEEKVEMEGNKRAKPNACVVCLGALQDCYMVPKLDQVVASIINSGYDADKFSLSLSLPICLSLRQHSLLVHLATRLPASCMAGMEPEDIVPIKQVWKYIYPDLVAREVSLEHQTGDVTDFFAELQLEWPEDSKELSCMVTLCKEEYATRAKNIHVYNMGIFSRQGVEKSLTGVTSAQFIENYPVPPEVPSESFQLSMKMFRSSVYIGGRYCKYDRELPQTPWFINGVRMRETSIEEVISERMNERVKAAEMKFLASGREDVDVRMLGSGRPFAFECVNPRKTRFTHEELLELEDHINSHHAGVKVNSLKVVTKEDIKKLKEGEEEKRKRYTALCVT---AEPCQDIMFCKLEKMTDLTLQQETPIRVLHRRSNAIREKVVHNMKVE---REGMKDNIFKLLVSTSAGTYVKEFVHGDFSRTVPNIGSLLGCSSADILALDVEEVELAWPPGG 1545          

BLAST of EMLSAG00000000629 vs. C. finmarchicus
Match: gi|592824267|gb|GAXK01130301.1| (TSA: Calanus finmarchicus comp70521_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 94.7449 bits (234), Expect = 4.698e-22
Identity = 46/98 (46.94%), Postives = 65/98 (66.33%), Query Frame = 0

Query:  363 SKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGC-DIDIEALDVQAINLDWPPGS 459
            + +TL Q+TP+RVLHRRSNA+R++ V ++K E   +E    +  + + S      VKEFVHGDF RT P++ S++GC   DI ALDV+ + L WPPG 
Sbjct:  100 TDLTLQQETPIRVLHRRSNAIREKVVHNMKVE---REGMKDNIFKLLVSTSAGTYVKEFVHGDFSRTVPNIGSLLGCSSADILALDVEEVELAWPPGG 384          

BLAST of EMLSAG00000000629 vs. C. finmarchicus
Match: gi|592839949|gb|GAXK01117595.1| (TSA: Calanus finmarchicus comp3618049_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 34.6538 bits (78), Expect = 1.913e-1
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = 0

Query:  107 IFLTEKIEKFRSDDIIPIKQVWKWLFP 133
            + L  ++   +S+DI+PIKQVWK ++P
Sbjct:  267 VHLASRMAGMQSEDILPIKQVWKKIYP 347          

BLAST of EMLSAG00000000629 vs. C. finmarchicus
Match: gi|592839950|gb|GAXK01117594.1| (TSA: Calanus finmarchicus comp3618049_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 33.113 bits (74), Expect = 1.286e+0
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = 0

Query:  107 IFLTEKIEKFRSDDIIPIKQVWKWLFP 133
            + L  ++   +S+DI+PIKQVWK ++P
Sbjct:  501 VHLASRMAGMQSEDILPIKQVWKKIYP 581          

BLAST of EMLSAG00000000629 vs. L. salmonis peptides
Match: EMLSAP00000000629 (pep:novel supercontig:LSalAtl2s:LSalAtl2s109:774756:806135:1 gene:EMLSAG00000000629 transcript:EMLSAT00000000629 description:"maker-LSalAtl2s109-snap-gene-8.30")

HSP 1 Score: 956.822 bits (2472), Expect = 0.000e+0
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query:    1 MTLSYKESVFQALEEAQDICFRCKLRYSGLREVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLFESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPGSMDNSNNVVS 468
            MTLSYKESVFQALEEAQDICFRCKLRYSGLREVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLFESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPGSMDNSNNVVS
Sbjct:    1 MTLSYKESVFQALEEAQDICFRCKLRYSGLREVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLFESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPGSMDNSNNVVS 468          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|121942830|sp|Q3MIT2.1|PUS10_HUMAN (RecName: Full=Putative tRNA pseudouridine synthase Pus10; AltName: Full=Coiled-coil domain-containing protein 139; AltName: Full=tRNA pseudouridine 55 synthase; Short=Psi55 synthase; AltName: Full=tRNA pseudouridylate synthase; AltName: Full=tRNA-uridine isomerase)

HSP 1 Score: 248.44 bits (633), Expect = 2.749e-75
Identity = 145/456 (31.80%), Postives = 238/456 (52.19%), Query Frame = 0

Query:   37 RTSEAQNGSEG-KISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEK----FRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSL----------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVV-----VIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPG 458
            R S +  GS   K S  N C+ C+G+LQ+      +++V Q ++ S ++      ++S P  LS+RE +  + + +++ K       DDI+ +K+ +KW+       E    + G S F   +   + E  ++ + ++ +C + +     K  K    + TR  + K+L  +   DF   +  PPN P                        +  NLPQTPWIIDG RKL++S+EE+I +    +FK +   F SSGREDVD R LG+GRPFA E VNP +  F+ +E++ ++  IN+S+  +++  L L+ +  +  +KEGE++KTKTY +++     +   D+E   D+ + K       + QKTP+RVLHRR  A+R R +  +       E     +  F   LK +    +KEFVHGDF RT P++ S++    DI  LDV+++++DWPP 
Sbjct:   90 RISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCF-----KPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLK-------IDQKTPLRVLHRRPLAVRARVIHFM-------ETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPA 526          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|81905037|sp|Q9D3U0.1|PUS10_MOUSE (RecName: Full=Putative tRNA pseudouridine synthase Pus10; AltName: Full=Coiled-coil domain-containing protein 139; AltName: Full=tRNA pseudouridine 55 synthase; Short=Psi55 synthase; AltName: Full=tRNA pseudouridylate synthase; AltName: Full=tRNA-uridine isomerase)

HSP 1 Score: 239.965 bits (611), Expect = 4.098e-72
Identity = 136/444 (30.63%), Postives = 230/444 (51.80%), Query Frame = 0

Query:   48 KISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEK----FRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSL----------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVV-----VIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPG 458
            K SK   C+ C+G+LQ+      + +V Q ++ S ++     +++S P  LS+RE +  + + +++ K       +D++ +K+ +KW+       E      G S F   +   + E  ++ + +  +C++ +     K  K    + TR  + K+L  +   DF   +  PPN P                        +  NLPQTPWIIDG RK+++S+EE+I +    +F+ +   F SSGREDVD R LG+GRPFA E +NP +  F+ +E++ ++  IN S+  +++  L L+ +  +  +KEGE++KTKTY +++     +   D+    D+ + K       + QKTP+RVLHRR  A+R R +  +K            +  F   LK +    +KEFVHGDF RT P+L S++    DI  LDV+++++DWPP 
Sbjct:  100 KPSKLRVCNVCLGILQEFCEKGFITKVCQKVEASGFEFTSVVLSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRNDVVQLKEAYKWITHPLFSEELGVPTDGKSLFEVSVVFAHPETAEDCHFLGEVCRDCF-----KPAKNKQSVFTRMAVLKALSKIKEEDFLGQFPCPPNSPKTVCTVLEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGERKMESSVEELISDHLLAVFRAESFNFSSSGREDVDVRTLGNGRPFAVELLNPHRVHFTSQEMKELQQTINKSSDKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNRAIQKKDIGFLDDLKDLK-------IDQKTPLRVLHRRPLAVRTRAIHSMKTHYL-------DEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNLGSLMNVTADILELDVESVDVDWPPA 524          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|82184788|sp|Q6ING2.1|PUS10_XENLA (RecName: Full=Putative tRNA pseudouridine synthase Pus10; AltName: Full=Coiled-coil domain-containing protein 139; AltName: Full=tRNA pseudouridine 55 synthase; Short=Psi55 synthase; AltName: Full=tRNA pseudouridylate synthase; AltName: Full=tRNA-uridine isomerase)

HSP 1 Score: 235.728 bits (600), Expect = 1.270e-70
Identity = 146/437 (33.41%), Postives = 238/437 (54.46%), Query Frame = 0

Query:   55 CSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEK----FRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSL----------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMT-LDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPGSMDN 462
            C  C+G+LQ       +E+V   I ++ Y+ KDF ++ISLP  LS+RE S  +   +++ K        DI+ +K+ +KW+    L  +        S F   +   + E D + + ++ +C++ +     K  K    + TR  + K+L+ +   DFR  +  PP+ P                        +  NLPQTPWIIDG RK+++S+EE+I +     F+     F SSGREDVD R LG GRPFA E +NP K  F+ +E+++++  IN+S + +++  L ++ +  +  +KEGE++KTK Y +++ I   V    L +L+G     ++T++QKTP+RVLHRR  A R RT+  ++ E          +  F   LK +    +KEFVHGDF RT P++ SI+  + DI  LDV+++++DWPP S+D+
Sbjct:   96 CPLCLGILQQFCEPEFIEKVFVKINSAVYELKDFVLSISLPAQLSVREHSAWLQAKQEMGKHGHSLDKTDIVQLKEAYKWIIHPLLSDQLGIQADSKSLFEVAVVFTHPETDGDCHFLATVCRDCF-----KPTKNKQSVFTRMAVVKALEKIKEEDFRVQFPFPPSSPETTCEVVDIQCNHSPVFVAGRYNKYSRNLPQTPWIIDGERKIESSVEELITDHLVAAFRSSSFNFSSSGREDVDVRTLGKGRPFAIELLNPHKVQFTGQEIKALQQKINTSDK-IKVRDLQIVSREAVAHMKEGEEEKTKCYCALIWIEKMVRNEDLQLLDG---LEELTIAQKTPLRVLHRRPLASRSRTIHTMRTEYV-------DEHHFRLYLKTQAGTYIKEFVHGDFGRTKPNVGSIMETNADILELDVESVDVDWPP-SLDD 515          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|74870264|sp|Q9VPK7.2|PUS10_DROME (RecName: Full=Putative tRNA pseudouridine synthase Pus10; AltName: Full=Coiled-coil domain-containing protein 139 homolog; AltName: Full=tRNA pseudouridine 55 synthase; Short=Psi55 synthase; AltName: Full=tRNA pseudouridylate synthase; AltName: Full=tRNA-uridine isomerase)

HSP 1 Score: 187.963 bits (476), Expect = 1.203e-52
Identity = 145/498 (29.12%), Postives = 241/498 (48.39%), Query Frame = 0

Query:   19 ICFRCKLRYSGLREVYKYRT-----------------SEAQNGSEG----KISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEK----IEKFRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHM--GLVTRQ--------------------GIEKSLQSVSNSDFRDSYSVPPNIPSLFESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIF--KFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPGSMDNSNN 465
            +C  C+LRY   R   +YR                  +E    SEG    K ++   CS C+G+    F    L  +  S   ++YD +   +AI LP+SL LR+ ++   L  +    I++    D+ PIK+  K +    +  EK   +   +     + + ++ + +E+  + ++ KE +  +     +  +  GL+ +Q                     +E+S++ +++ D +                L  TPWI++G R ++ SIEEIII      F  K     F SSGREDVD R LG GRPF  E  N +++  + +E  ++E A+++S   V I+ L ++ + +L  +K GE+QK K YR++  +   V  ++ +L     ++   + QKTP+RVLHRR    R RT+  +       ++  G+ +  I  +  +    +KE VHG+F RTTPSL SIIG  +DI+ALDV  I+LDW P  +DNS +
Sbjct:   15 VCEICQLRYLKARGA-EYRNINETLQKLDVKLNENVENEELVISEGSQPSKRARLGVCSTCLGLFSKDFQNELLSSILAS-DFAKYDCQKIVLAICLPMSLQLRQLAMWFALRRRFGPSIDENNPPDV-PIKEAVKLIL-HPIICEKLAKVYDANGLMINIDLTHSLEGEEVEKLVKLNKEAFPAKASHQKRIEISRGLLEKQYQPSKVKAETYEKYFQIPCSNVEESIK-LTSIDLQGPLICVAGRYRKLSRELSHTPWILNGQRLMEDSIEEIIIRHVGPHFTEKLGKITFMSSGREDVDVRCLGKGRPFVLEIPNAKRSCLTKDEAFAMERAVDASGM-VSIHHLQVVPREELTHIKTGEEQKKKFYRALCALQEPV--SVKILEQLQISASFNIQQKTPIRVLHRRPLHTRPRTIYSVT-----AKVHRGNPKALIIDIVTQAGTYIKELVHGEFGRTTPSLSSIIGKPMDIQALDVVGIDLDW-PHEVDNSKH 498          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|332319823|sp|A1RX33.1|PUS10_THEPD (RecName: Full=tRNA pseudouridine synthase Pus10; AltName: Full=tRNA pseudouridine 54/55 synthase; Short=Psi54/55 synthase)

HSP 1 Score: 120.553 bits (301), Expect = 1.219e-28
Identity = 81/234 (34.62%), Postives = 123/234 (52.56%), Query Frame = 0

Query:  222 FESNLPQTPWIIDGVR-KLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQD-VRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAI 451
            +   LPQ PW     R K +TSIEE+I++P  ++F+ + AKF+++GRED+D R LG+GRPF  E   PRK    + +L+ +   INS     + +  L+   +  +K LK       K Y + V    P D E   ++       S   ++Q+TP RVLHRR + LRK+ V  L+     ++     +  F    +    VKEF+HGD  RTTPS+   +G ++    LDV +I
Sbjct:  217 YARGLPQNPWPQPDERIKFNTSIEELIVKPALELFEAEKAKFHAAGREDIDVRTLGTGRPFVLEIKKPRK---RNIDLKVLAEKINSGAGGLIEVLDLAYTDRKTIKKLKSLASIAKKAYVARVKFEKPVDDEKLAEISK---VFSNAVINQRTPTRVLHRRVDKLRKKIVYRLEARKISQD-----EVEFYLETQGGFYVKEFIHGDNGRTTPSIAEFLGNNVLSIELDVVSI 439          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|74513032|sp|O29113.1|PUS10_ARCFU (RecName: Full=tRNA pseudouridine synthase Pus10; AltName: Full=tRNA pseudouridine 54/55 synthase; Short=Psi54/55 synthase)

HSP 1 Score: 108.227 bits (269), Expect = 9.439e-25
Identity = 83/242 (34.30%), Postives = 127/242 (52.48%), Query Frame = 0

Query:  225 NLPQTPWII-----DGV-------RKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAIN 452
            N+ QT W+      +G        +K  +S+EE+I  P  ++FK  DAK + +GREDVD RMLG+GRPF  E + PRK      +L+ +E AINS    V +  L       ++ +K   ++  KTYR+ VV    VE   + L   L + K  + Q+TP+RV HRR++ +R R + D +       L + + R  +   + E    +KE V GD  RT PSL   +G +  ++ LDV A++
Sbjct:  131 NISQTRWLCGYCNGEGCEVCNFTGKKYVSSVEELIAMPAVRLFKARDAKLHGAGREDVDARMLGTGRPFVLEVIEPRKRFV---DLKELEEAINSQKW-VAVRDLEYTDAEKVREVK--TERHRKTYRAKVVFEEKVER--ERLIEALESLKGEIRQRTPMRVSHRRADRVRVRRLYDAR-------LIHHTGRVAVVEFEAEAGLYIKELVSGDNGRTRPSLAEKVGVNARVDRLDVIAVS 357          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|74558834|sp|Q8TUV7.1|PUS10_METKA (RecName: Full=tRNA pseudouridine synthase Pus10; AltName: Full=tRNA pseudouridine 54/55 synthase; Short=Psi54/55 synthase)

HSP 1 Score: 104.375 bits (259), Expect = 4.476e-23
Identity = 78/240 (32.50%), Postives = 119/240 (49.58%), Query Frame = 0

Query:  225 NLPQTPWIIDGVR-----------KLDT-SIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSST-QDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFI-FSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDV 448
             +PQT W     R           KL T S+EE+I    K  F  +  KF+++GRED+D RMLG+GRPF  E + P++    + +L+ +E  IN     DV++  L      D+  +K+  ++  K YR+ V    P   +   +VL G     +  + Q+TP RVLHRR++ +R++ V + K       +    DR  I F       VKE + GD  RT PSL  ++  + + E LDV
Sbjct:  198 GIPQTKWPCPRCRGAGCPNCDFTGKLYTESVEELIGMVLKDAFLAESHKFHAAGREDIDVRMLGNGRPFVMELLYPKR---RNVDLKEIEGEINRKVGDDVQVVGLEYGDPEDVGKVKDLSERSRKRYRAWVKFGKPVPEDKLREVLKG---LERSVIEQRTPRRVLHRRADKVRRKRVHEAKL------IEYDGDRAVIEFLCDPGLYVKELISGDAGRTRPSLAELVEVEAECERLDV 425          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|332319822|sp|D5TZL3.1|PUS10_THEAM (RecName: Full=tRNA pseudouridine synthase Pus10; AltName: Full=tRNA pseudouridine 54/55 synthase; Short=Psi54/55 synthase)

HSP 1 Score: 99.3673 bits (246), Expect = 2.307e-21
Identity = 73/229 (31.88%), Postives = 112/229 (48.91%), Query Frame = 0

Query:  225 NLPQTPWII-DGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDV-EMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAI 451
            N+   PW+   G RK   SI+E +       FK    K +++GREDVD R LG GRP   E V P   +    ++  V   I S   +VR++  +   + D++ LK+   +K K YR +VV  + + E  L+ L      + + + Q+TP+R+L R+ + LR R V    +E   K +S+ S    ++       VKE VH D  RT P   SI+  ++    LDV  I
Sbjct:  223 NISHVPWLAKQGGRKYPLSIQEYLESRLADPFKAKKVKIHAAGREDVDARTLGPGRPLVIEIVEP---LVRSYDIEDVNKLIRSDLVEVRVHKPA--SRRDIELLKQTSREKRKVYRLLVVSSTPISEAQLNELEAYF--NDIAIQQRTPIRILTRKKDVLRVRKV----YEVRTKPVSSTSFEALVYC-DGGLYVKELVHCDQGRTNPCFASILNTELKPVELDVLYI 439          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|2495757|sp|Q60346.1|PUS10_METJA (RecName: Full=tRNA pseudouridine synthase Pus10; AltName: Full=tRNA pseudouridine 54/55 synthase; Short=Psi54/55 synthase)

HSP 1 Score: 96.2857 bits (238), Expect = 2.552e-20
Identity = 80/242 (33.06%), Postives = 112/242 (46.28%), Query Frame = 0

Query:  225 NLPQTPWIIDGVR------------KLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRF--IFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAIN 452
             +PQT W     R            K   S+EEII +PF +  K  DAKF+ +GRED+D RMLG GRPF  E   P+       +L  +   IN   + V + +L   ++ D    K    +  KTYR++V   SD     ++   +      T+ QKTP RVLHRR++  R R V  +K        S   D  F  I        +KE + GD  RT PS+ SI+  +   + LDV  I+
Sbjct:  219 GIPQTRWPCRKCRGKGCELCNYTGKKYPISVEEIIAKPFLEATKGVDAKFHGAGREDIDVRMLGDGRPFVLEIKEPK---IRKIDLNKIAEEINKDGR-VEVLNLEFGVRKDKVIFKNTPHR--KTYRALVEC-SDKITDEELKLLEKELENRTIYQKTPKRVLHRRADLERIRKVYKVK-------TSKVDDNHFEMIIYCDGGLYIKELISGDDGRTNPSVSSILNKNCICKELDVLKIH 446          

BLAST of EMLSAG00000000629 vs. SwissProt
Match: gi|74543374|sp|Q9HIN9.1|PUS10_THEAC (RecName: Full=tRNA pseudouridine synthase Pus10; AltName: Full=tRNA pseudouridine 54/55 synthase; Short=Psi54/55 synthase)

HSP 1 Score: 95.5153 bits (236), Expect = 3.064e-20
Identity = 69/230 (30.00%), Postives = 112/230 (48.70%), Query Frame = 0

Query:  222 FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAI 451
            F  ++PQT WI    R    ++E +I   F +     +   + SGREDVD RMLG+GR F  E  NPR   F   EL  V   IN+S + V I  +       +  +K   ++  K Y ++VV    ++ +  +L   +  +   + Q+TP+RV  RRS+ +R R +  +          + ++   + S +    +KE V+GD  RT PSL  + G  ++++ LDV  I
Sbjct:  169 FRRDMPQTRWI---HRPNGDTVESVIGSVFTRYAGGTNYYLHGSGREDVDVRMLGNGREFVLEVENPRYREF---ELDPVVKEINTSGKGVEIFDVKFSSHDAVSEVK--LEKHRKVYDALVVSDRPIDESR-LLEACINLTGKNIYQRTPLRVAQRRSDLVRTRRIDQVDLVGV-----SANEAEILISAEAGTYIKELVNGDGGRTRPSLSEMYGSPLNVKELDVIKI 384          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: EEB10994.1 (conserved hypothetical protein [Pediculus humanus corporis])

HSP 1 Score: 279.641 bits (714), Expect = 7.747e-88
Identity = 182/500 (36.40%), Postives = 252/500 (50.40%), Query Frame = 0

Query:   17 QDICFRCKLRYSGLREVYKYRTSEAQNGSEGKI-----------------SKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDDIIP-----------IKQVWKWLFPTELKA--EKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLF----------------------ESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSD----VEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLK--QEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPG 458
             ++C  C LRY  L E    + S + N  +G+I                 +K NPCSAC+G+LQ       L  V  S+  S YD  D + A+SLPIS  +R RS+ + L  K ++F  +   P           IK+VWKWL   ++     K    G   +F   + I Y +D+ E  S+ +M  EE+  R     K+H  ++TR+ +EK L       F + Y VPP  P+ F                        NL QTPW+I+G R++++S+EEII +P     K    KF SSGREDVD R LG+GRPF  E +NPRK     + +  +E  IN ST DV +  L ++ K DL  +K GE+ KTKTY    +I  D    +    + LN       + ++Q+TP+RVLHRR  A+R RTV  LK  A   E+ N +   F  SLK      +KEFVHGDF RTTP+L  I+  + DI ALDV+ + L WPP 
Sbjct:   18 NNLCNYCILRY--LNE----KNSNSYNDVDGRIKKLLNAQDEDYLPEIKKTKLNPCSACLGLLQLSDSSEQLNTVINSVIESGYDCDDVTFALSLPISFLIRSRSIWLHLINKFKEFFENKYYPHEIKQEYLVVSIKEVWKWLSSQKIAKMIGKNANNGVDGNFHVNIHINYDDDNLETASLFKMFPEEFQYRNHHKRKFHGEILTRKNVEKMLLETDLKSFDNIYPVPPKTPNTFFQFSTVQCMHDSIYVAGRYNKLSRNLSQTPWMINGERRMESSVEEIIEKPISNYIKNSGTKFSSSGREDVDVRTLGNGRPFVVEILNPRKVKIDQQYMLEIEKEINKSTNDVFVRDLQIVQKKDLHHIKAGEETKTKTYTCFCLIKLDNNDEIPEIKEKLNNLSQIKDLVINQRTPIRVLHRRPLAVRPRTVYWLK--ADFHEIKN-NHLFFKLSLKVGAGTYIKEFVHGDFGRTTPNLSEILEKETDILALDVEEVALQWPPN 508          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: gb|KFM73523.1| (putative tRNA pseudouridine synthase Pus10, partial [Stegodyphus mimosarum])

HSP 1 Score: 244.973 bits (624), Expect = 1.523e-74
Identity = 162/513 (31.58%), Postives = 257/513 (50.10%), Query Frame = 0

Query:    6 KESVFQALEEAQ---DICFRCKLRYSGLREVYKYRT-------------------------SEAQNGSEGKISKAN------PCSACIGVLQDHF--MLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIE---KFRSDDIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIP----------------------SLFESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPP 457
            K S FQA++E     ++C RC LR+ G++E   Y T                         +E    SE +I   +      PC+AC+G+LQ+    ML  +EE S     S ++  DF + +S P+ L LR+R   ++L E+++        +I  +K VWKW+  ++L    K      S+F   L + Y +   E   + + C E +  R KK       + TR  I K+++ VS S+    ++ PP+ P                      + +   L QTPWI+DG R++D+S+ E+I +  ++    D   F +SGREDVD +MLG+GRPF  E +NPR+   S  E++ +E  IN+ST  + +  L ++ K D   LKEGE+QK KTY ++ ++     ++ + ++  L    + + QKTP+RVLHRR+ A R+R +  +  +     L +          +    VKEFVHGDF RT P+L  ++  + DI  LDV+ I+LDWPP
Sbjct:    3 KSSDFQAIKECLEPLNVCNRCILRFFGMKEYTSYVTKSSIKKTFQQLLDEKICELPAAKHETEDLVASEEEICPPHKKHHFLPCAACLGILQESCDDMLAKVEEASAK---SGHEFDDFVLQLSFPVVLDLRDRLFHLYLQEQLKDSYTILCSEIPSVKDVWKWVIGSQLGKITKTDFTPCSNFQISLLVTYPDTAKECFKLFQKCPEAFPNR-KKRKATEEEIYTRAAILKAVELVS-SEINSLFTFPPSPPDKTCIFHDILCTRSPLYLGGRYNKYSRELSQTPWIVDGERRMDSSVNELITDVVQRRICADKIVFSASGREDVDVKMLGNGRPFVLEILNPRRIKLSDAEMKDIENEINASTDSIAVKDLQIITKQDTLLLKEGEEQKRKTYSAICIVKK--TLSQNDIDKLLLLKDLKIQQKTPIRVLHRRNLATRERLLHSIDAKLLDSHLLH-----LKLETQAGTYVKEFVHGDFGRTLPNLGMLLDTEADILELDVECIHLDWPP 503          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: EFX74077.1 (hypothetical protein DAPPUDRAFT_324687 [Daphnia pulex])

HSP 1 Score: 240.35 bits (612), Expect = 8.285e-74
Identity = 136/388 (35.05%), Postives = 219/388 (56.44%), Query Frame = 0

Query:   20 CFRCKLRYSGLR--EVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPCL-EEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEK-----FRSDDIIPIKQVWKWLFP-TELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVP-PNIPSL-------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMT---LDVLNGKLPASKVTLSQKTPVRV 375
            C+RC +R++  R  E Y+ +  + +   E K+ K +PC  C+G++QD F+  C+ +E+SQ +  S+YDS  FS  +SLP+++ LRE+++   +TEK  +     F+ D +  +K VWK++F        KK L   +S F  ++ + Y ++  E  ++  MC E++  R K++  Y  G+ TR      L  +S+ +F+  Y++  PN+ +                    F   L QTPW+I+G + +DTSI+E+I +  +  FK    KF SSGREDVD R LG GRPFA E ++P +T+F+  E+  V+  IN ST  ++I  L L+ K  L  LKEGE++K+K YR++ V      +T   LD +NG    +++ L QKTP+RV
Sbjct:   26 CWRCAIRFTRERKPEAYQQKNLDLKQEPEEKVQKTSPCVICLGLVQDQFIKECITDEISQRVIESDYDSPTFSCTVSLPVAIMLREQAVWFLVTEKFPELLSTIFKIDHVTSLKDVWKFVFSEALELKLKKKLEYSSSSFQIQVNVLYQDEISECGALVDMCPEKFKHRYKQTRVYREGIFTRNAAHSVLSGISSLNFKKHYTLKVPNVGTHYGVVQCSHTSIHIAGRYQKFSRKLCQTPWLIEGEKHMDTSIQELICKHLEPFFKTQSVKFSSSGREDVDVRTLGLGRPFAIELLDPHRTLFTQVEITQVQIIINKSTDAIQIRDLQLVTKEQLSVLKEGEEEKSKIYRALCVTLDGSTLTAEDLDRING---TTELVLMQKTPLRV 410          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: XP_394978.3 (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1 [Apis mellifera])

HSP 1 Score: 241.121 bits (614), Expect = 4.708e-73
Identity = 165/489 (33.74%), Postives = 250/489 (51.12%), Query Frame = 0

Query:   17 QDICFRCKLRYSGLREVYKY----RTSEAQNGSEGKISKANP---CSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLFP--TELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY---SVPPNIPSLFES-------------------NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAF-PKELSN------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            Q+ C RC  R+ G R +  +    + ++     E K    N    C AC+G+LQ+      + ++   IK   YDS  F+ A+++PI +SLRER L I    ++    +        +  +K VWKW+     E   +K+  L   S F  E+ + Y  ++ E  ++ ++  E   M  K   KY     +++ IE  +  +++ +F   +   S   N   L E+                    L QTPW I+G +K+ TS+++I+  P   + K    KF SSGREDVD R + SGRPFA E +NPR T  + + L  +   IN S+Q V+I S L +L + DLK LKEGE+ KTK YR++ +    S   ++L+ LN       + + QKTP+RVLHRR  + R+R + +++     P+EL N       ++  FI  +K +    VKEFVHGDF RT PSL  I+  +IDI ALDV  INL+WP
Sbjct:   18 QNCCLRCCFRFIGWRTLNCFEDPIKFAKHVRYIETKDISFNDEILCMACLGILQNKIQEQVITKIQAEIKKQNYDSGTFTCALTIPICISLRERFLRIQCATQLNLSENTLLDLKVKLQNVKDVWKWIMTPKIEQAIKKQVDLITPSPFLIEIILTYKSNEKECETLLKIYNELSNMGNKSKRKYDDNKFSKKNIETIMTKITDKEFSQHFKALSFETNNSILIENIMCSHSSIFIGGRYNKLSRELSQTPWFINGEKKMQTSVQDILCNPIADVTKAQSIKFLSSGREDVDVRNIYSGRPFAVELINPRLTKITEKLLSDLINKINLSSQQVQIVSNLKVLSRFDLKKLKEGENVKTKFYRALCICRNASKNVLSLEKLNN---LKHLKIIQKTPMRVLHRRPLSPRERLIYEMRARWVKPQELKNLNISSEDANIFFILDIKTQAGTYVKEFVHGDFGRTKPSLCDILNVEIDIVALDVTGINLNWP 503          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: XP_006566712.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2 [Apis mellifera])

HSP 1 Score: 236.113 bits (601), Expect = 3.055e-71
Identity = 155/444 (34.91%), Postives = 233/444 (52.48%), Query Frame = 0

Query:   55 CSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLFP--TELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY---SVPPNIPSLFES-------------------NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAF-PKELSN------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            C AC+G+LQ+      + ++   IK   YDS  F+ A+++PI +SLRER L I    ++    +        +  +K VWKW+     E   +K+  L   S F  E+ + Y  ++ E  ++ ++  E   M  K   KY     +++ IE  +  +++ +F   +   S   N   L E+                    L QTPW I+G +K+ TS+++I+  P   + K    KF SSGREDVD R + SGRPFA E +NPR T  + + L  +   IN S+Q V+I S L +L + DLK LKEGE+ KTK YR++ +    S   ++L+ LN       + + QKTP+RVLHRR  + R+R + +++     P+EL N       ++  FI  +K +    VKEFVHGDF RT PSL  I+  +IDI ALDV  INL+WP
Sbjct:   57 CMACLGILQNKIQEQVITKIQAEIKKQNYDSGTFTCALTIPICISLRERFLRIQCATQLNLSENTLLDLKVKLQNVKDVWKWIMTPKIEQAIKKQVDLITPSPFLIEIILTYKSNEKECETLLKIYNELSNMGNKSKRKYDDNKFSKKNIETIMTKITDKEFSQHFKALSFETNNSILIENIMCSHSSIFIGGRYNKLSRELSQTPWFINGEKKMQTSVQDILCNPIADVTKAQSIKFLSSGREDVDVRNIYSGRPFAVELINPRLTKITEKLLSDLINKINLSSQQVQIVSNLKVLSRFDLKKLKEGENVKTKFYRALCICRNASKNVLSLEKLNN---LKHLKIIQKTPMRVLHRRPLSPRERLIYEMRARWVKPQELKNLNISSEDANIFFILDIKTQAGTYVKEFVHGDFGRTKPSLCDILNVEIDIVALDVTGINLNWP 497          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: XP_006566716.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X3 [Apis mellifera])

HSP 1 Score: 233.032 bits (593), Expect = 9.249e-71
Identity = 156/442 (35.29%), Postives = 234/442 (52.94%), Query Frame = 0

Query:   57 ACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLF-PTELKAEKKYL-LGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY---SVPPNIPSLFES-------------------NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAF-PKELSN------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            AC+G+LQ+      + ++   IK   YDS  F+ A+++PI +SLRER L I    ++    +        +  +K VWKW+  P   +A KK + L   S F  E+ + Y  ++ E  ++ ++  E   M  K   KY     +++ IE  +  +++ +F   +   S   N   L E+                    L QTPW I+G +K+ TS+++I+  P   + K    KF SSGREDVD R + SGRPFA E +NPR T  + + L  +   IN S+Q V+I S L +L + DLK LKEGE+ KTK YR++ +    S   ++L+ LN       + + QKTP+RVLHRR  + R+R + +++     P+EL N       ++  FI  +K +    VKEFVHGDF RT PSL  I+  +IDI ALDV  INL+WP
Sbjct:    2 ACLGILQNKIQEQVITKIQAEIKKQNYDSGTFTCALTIPICISLRERFLRIQCATQLNLSENTLLDLKVKLQNVKDVWKWIMTPKIEQAIKKQVDLITPSPFLIEIILTYKSNEKECETLLKIYNELSNMGNKSKRKYDDNKFSKKNIETIMTKITDKEFSQHFKALSFETNNSILIENIMCSHSSIFIGGRYNKLSRELSQTPWFINGEKKMQTSVQDILCNPIADVTKAQSIKFLSSGREDVDVRNIYSGRPFAVELINPRLTKITEKLLSDLINKINLSSQQVQIVSNLKVLSRFDLKKLKEGENVKTKFYRALCICRNASKNVLSLEKLNN---LKHLKIIQKTPMRVLHRRPLSPRERLIYEMRARWVKPQELKNLNISSEDANIFFILDIKTQAGTYVKEFVHGDFGRTKPSLCDILNVEIDIVALDVTGINLNWP 440          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: XP_006566715.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X3 [Apis mellifera])

HSP 1 Score: 233.032 bits (593), Expect = 9.249e-71
Identity = 156/442 (35.29%), Postives = 234/442 (52.94%), Query Frame = 0

Query:   57 ACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLF-PTELKAEKKYL-LGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY---SVPPNIPSLFES-------------------NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAF-PKELSN------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            AC+G+LQ+      + ++   IK   YDS  F+ A+++PI +SLRER L I    ++    +        +  +K VWKW+  P   +A KK + L   S F  E+ + Y  ++ E  ++ ++  E   M  K   KY     +++ IE  +  +++ +F   +   S   N   L E+                    L QTPW I+G +K+ TS+++I+  P   + K    KF SSGREDVD R + SGRPFA E +NPR T  + + L  +   IN S+Q V+I S L +L + DLK LKEGE+ KTK YR++ +    S   ++L+ LN       + + QKTP+RVLHRR  + R+R + +++     P+EL N       ++  FI  +K +    VKEFVHGDF RT PSL  I+  +IDI ALDV  INL+WP
Sbjct:    2 ACLGILQNKIQEQVITKIQAEIKKQNYDSGTFTCALTIPICISLRERFLRIQCATQLNLSENTLLDLKVKLQNVKDVWKWIMTPKIEQAIKKQVDLITPSPFLIEIILTYKSNEKECETLLKIYNELSNMGNKSKRKYDDNKFSKKNIETIMTKITDKEFSQHFKALSFETNNSILIENIMCSHSSIFIGGRYNKLSRELSQTPWFINGEKKMQTSVQDILCNPIADVTKAQSIKFLSSGREDVDVRNIYSGRPFAVELINPRLTKITEKLLSDLINKINLSSQQVQIVSNLKVLSRFDLKKLKEGENVKTKFYRALCICRNASKNVLSLEKLNN---LKHLKIIQKTPMRVLHRRPLSPRERLIYEMRARWVKPQELKNLNISSEDANIFFILDIKTQAGTYVKEFVHGDFGRTKPSLCDILNVEIDIVALDVTGINLNWP 440          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: XP_006566714.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X3 [Apis mellifera])

HSP 1 Score: 233.032 bits (593), Expect = 9.249e-71
Identity = 156/442 (35.29%), Postives = 234/442 (52.94%), Query Frame = 0

Query:   57 ACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLF-PTELKAEKKYL-LGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY---SVPPNIPSLFES-------------------NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAF-PKELSN------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            AC+G+LQ+      + ++   IK   YDS  F+ A+++PI +SLRER L I    ++    +        +  +K VWKW+  P   +A KK + L   S F  E+ + Y  ++ E  ++ ++  E   M  K   KY     +++ IE  +  +++ +F   +   S   N   L E+                    L QTPW I+G +K+ TS+++I+  P   + K    KF SSGREDVD R + SGRPFA E +NPR T  + + L  +   IN S+Q V+I S L +L + DLK LKEGE+ KTK YR++ +    S   ++L+ LN       + + QKTP+RVLHRR  + R+R + +++     P+EL N       ++  FI  +K +    VKEFVHGDF RT PSL  I+  +IDI ALDV  INL+WP
Sbjct:    2 ACLGILQNKIQEQVITKIQAEIKKQNYDSGTFTCALTIPICISLRERFLRIQCATQLNLSENTLLDLKVKLQNVKDVWKWIMTPKIEQAIKKQVDLITPSPFLIEIILTYKSNEKECETLLKIYNELSNMGNKSKRKYDDNKFSKKNIETIMTKITDKEFSQHFKALSFETNNSILIENIMCSHSSIFIGGRYNKLSRELSQTPWFINGEKKMQTSVQDILCNPIADVTKAQSIKFLSSGREDVDVRNIYSGRPFAVELINPRLTKITEKLLSDLINKINLSSQQVQIVSNLKVLSRFDLKKLKEGENVKTKFYRALCICRNASKNVLSLEKLNN---LKHLKIIQKTPMRVLHRRPLSPRERLIYEMRARWVKPQELKNLNISSEDANIFFILDIKTQAGTYVKEFVHGDFGRTKPSLCDILNVEIDIVALDVTGINLNWP 440          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: XP_006566713.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X3 [Apis mellifera])

HSP 1 Score: 233.032 bits (593), Expect = 9.249e-71
Identity = 156/442 (35.29%), Postives = 234/442 (52.94%), Query Frame = 0

Query:   57 ACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLF-PTELKAEKKYL-LGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY---SVPPNIPSLFES-------------------NLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAF-PKELSN------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            AC+G+LQ+      + ++   IK   YDS  F+ A+++PI +SLRER L I    ++    +        +  +K VWKW+  P   +A KK + L   S F  E+ + Y  ++ E  ++ ++  E   M  K   KY     +++ IE  +  +++ +F   +   S   N   L E+                    L QTPW I+G +K+ TS+++I+  P   + K    KF SSGREDVD R + SGRPFA E +NPR T  + + L  +   IN S+Q V+I S L +L + DLK LKEGE+ KTK YR++ +    S   ++L+ LN       + + QKTP+RVLHRR  + R+R + +++     P+EL N       ++  FI  +K +    VKEFVHGDF RT PSL  I+  +IDI ALDV  INL+WP
Sbjct:    2 ACLGILQNKIQEQVITKIQAEIKKQNYDSGTFTCALTIPICISLRERFLRIQCATQLNLSENTLLDLKVKLQNVKDVWKWIMTPKIEQAIKKQVDLITPSPFLIEIILTYKSNEKECETLLKIYNELSNMGNKSKRKYDDNKFSKKNIETIMTKITDKEFSQHFKALSFETNNSILIENIMCSHSSIFIGGRYNKLSRELSQTPWFINGEKKMQTSVQDILCNPIADVTKAQSIKFLSSGREDVDVRNIYSGRPFAVELINPRLTKITEKLLSDLINKINLSSQQVQIVSNLKVLSRFDLKKLKEGENVKTKFYRALCICRNASKNVLSLEKLNN---LKHLKIIQKTPMRVLHRRPLSPRERLIYEMRARWVKPQELKNLNISSEDANIFFILDIKTQAGTYVKEFVHGDFGRTKPSLCDILNVEIDIVALDVTGINLNWP 440          

BLAST of EMLSAG00000000629 vs. Select Arthropod Genomes
Match: gb|EFA10881.1| (Putative tRNA pseudouridine synthase Pus10-like Protein [Tribolium castaneum])

HSP 1 Score: 223.787 bits (569), Expect = 8.761e-67
Identity = 171/491 (34.83%), Postives = 250/491 (50.92%), Query Frame = 0

Query:    1 MTLSYKESVFQALEEAQDICFRCKLRYSGLREVYKYRTSEAQNGSE--GKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD-DIIPIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTM------REKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSL----------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVI--PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT-VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPP 457
            MT S K  VF  L E    C RC LRY G      Y   + QNG +   K +K N C  C+ +LQD   L  + +  +     EYD K F   IS P+++ +RE S+ I + EK  KF  D +I+PI + W      +L  EK   LG   +  + L+I++       N+     KEE ++      R KKSN  + GL +R  I + L++++   F+   +VPP  P                        +  NL QTPW  D V+ +++S++EI+ +  + +FK     F SSGRED D RMLG GRPF  +  +P+ T    E+ R +E  I   T  + +  L  + +  L  +KEGE  K KTYR++ V+  P  +   ++ +N      +V L QKTP+RV HRRS  +R++ + ++  EA P  ++  S    +  L Q  T VKEFVHGD  RT PSL+ I+G  +DI ALDV  I+LD+PP
Sbjct:    1 MTESDKAEVFNFLLET-GCCKRCALRYVG-DNTADYDNPQ-QNGHDQLAKKTKLNSCIICLDLLQD-VTLESMVQCEELEGVKEYDCKTFINFISFPVAVLIREHSMEIHMNEKFPKFFEDRNIVPINRAWSLSIQNKL-GEK---LGKKFNKDSLLQIQF-------NTTYEFEKEESSIMFLMESRLKKSNGQNEGL-SRNVIGEFLKTINTELFKKHAAVPPEPPGKALSFDQVAYKHDYIFIAGRYCKYSRNLFQTPWANDEVQVINSSVQEILFKSLESVFKCSSLVFSSSGREDFDVRMLGRGRPFFVKICDPKVTTIPFEQFRLIEKQI-EETNLIEVRDLQFISRKLLNNIKEGEQFKQKTYRALCVVDKPEKIGEYIEKINN---MKRVKLLQKTPLRVYHRRSADVREKEIYNM--EARP--VTGASSLFELDVLTQAGTYVKEFVHGDLGRTRPSLREIMGGYVDIIALDVLDIHLDFPP 467          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|1022776077|gb|KZS19858.1| (putative tRNA pseudouridine synthase Pus10 [Daphnia magna])

HSP 1 Score: 299.671 bits (766), Expect = 1.137e-92
Identity = 173/469 (36.89%), Postives = 262/469 (55.86%), Query Frame = 0

Query:   20 CFRCKLRYSGLR--EVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPC-LEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKI-----EKFRSDDIIPIKQVWKWLFPTEL-KAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVP-PNIPSL-------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPP 457
            C+RC +R++  R  E Y+ +  E +   E K+ KA+PC  C+G++QDHF+    ++EV Q +   + DS  FS  +SLP+++ LRE+++   LTEK      +  + D +  +K VWK++F   L K   K L   +S F   + I Y ++  E  ++  +C E++  R  ++  Y  G+ TR   +  L  +S  DF+  Y++  PN+ +                    F   L QTPW+I+G + +DTSI+E+I +  +  FK    KF SSGREDVD R LG GRPFA E  +P +TIF+ +++  ++  IN+ST  ++I  L  + K  L  LK+GE++KTK YR++ V       T + L      +++ L QKTP+RVLHRR  A+R RTV ++K       L +     FI  LK +    +KEFVHGDF RT P+L  ++G  IDI ALDV A+ LDWPP
Sbjct:   51 CWRCAIRFTRERKPEAYQRKLVEFKLEPEEKLLKASPCVICLGLVQDHFIKEVFIDEVCQQVLKFDCDSATFSCMVSLPVAIMLREQAVWFLLTEKFPDLLSKNLKIDYVTSLKDVWKFVFTEALEKKLNKQLEHSSSPFQIHISILYKDEISECGALVDICPEKFKHRYNRTRVYREGIFTRNAAQSVLSGLSAEDFQKYYTLKLPNVSAHYGPVQCSHNSIHIAGRYQKFSRKLCQTPWLIEGEKHMDTSIQELICKHLEPFFKTQSVKFSSSGREDVDVRTLGRGRPFAIELTDPHRTIFTQDQITQLQNVINNSTDAIQIRDLQAVTKEQLSVLKQGEEEKTKIYRALCVTLDGSTPTEEDLAKINDITELVLMQKTPLRVLHRRPLAVRPRTVHEMK-----ATLLDDRPGHFILRLKTQAGTYIKEFVHGDFNRTVPNLCILLGRAIDIVALDVDAVELDWPP 514          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|998511988|ref|XP_015516548.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Neodiprion lecontei])

HSP 1 Score: 288.886 bits (738), Expect = 1.936e-88
Identity = 178/511 (34.83%), Postives = 265/511 (51.86%), Query Frame = 0

Query:   20 CFRCKLRYSGLREVYKYRTSEAQNGSEG-----------KISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDDII--------PIKQVWKWL-FPT--ELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSL----------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRI-NSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLK-------------FEAFPKELSN--GSDRR------------FIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            C RC LR SG+R    Y      + + G           ++   N C+AC+G+L D  +   +E+++  I   EYD   F+ A+++PI++ LRERSL I L++K E    D++I        PIK+VWKW+ FP    L A+    +  T  F  ++ + Y++D++E   +  MC + +  R ++  K+  G+ +R+ +E ++ S ++  F + Y  PP  P+                       +   L QTPW I G +K++TS++E++ +   K  K D  KF SSGREDVD R L SGRPFA E +NPR T F+   L+ +   IN ST  V I ++L ++ K  LKTLKEGED KTKTYR++ +  S     L  L+     + + + QKTPVRVLHRR  A R RT+  LK              +    E+ N    D +            F+  L  +    VKEFVHGDF RT PS+  I+  ++DI ALDV ++NL+WP
Sbjct:   21 CVRCCLRLSGVRSPTFYENPVQSSVTLGYKDDLVSEHVTEVKAKNGCTACLGILNDDAITAAIEKIASEINKQEYDCNTFTCALTIPIAVQLRERSLQINLSQKFE--FPDEVIASLKRTVPPIKEVWKWIAFPKIENLTAKMSLPISSTCPFVVDVFLVYSQDEEECKILLTMCGDTFKQRSQQKRKFSNGVFSRKSVETAMISTTDDQFVEHYPCPPTYPTSKASIDLVTCTHASFFIGGRYTKYSRELSQTPWFIGGEKKMETSVQELLCDQILKFVKADAMKFLSSGREDVDVRTLHSGRPFAVELLNPRVTKFTEARLKQLTEEINGSTNLVHIVDNLQVISKDQLKTLKEGEDTKTKTYRALCLCRS---RPLPSLDPINNINNLEIIQKTPVRVLHRRPLATRPRTIHSLKASWISSYEMAVLMCDTHDSEMDNHLALDHKLLNQVPDSEIDLFVLDLTTQAGTYVKEFVHGDFGRTKPSVCEILQSEVDIVALDVMSVNLNWP 526          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|817084490|ref|XP_012264898.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Athalia rosae])

HSP 1 Score: 285.034 bits (728), Expect = 4.038e-87
Identity = 179/505 (35.45%), Postives = 259/505 (51.29%), Query Frame = 0

Query:   20 CFRCKLRYSGLREVYKYR-------TSEAQNGSEGKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDD-----IIP-IKQVWKWLFPTELKA---EKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIP----------------------SLFESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRI-NSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFP-KELSNGSDR--------------------------RFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            C RC LR +G+R    Y        T    N S  ++    PC AC+G+L D  +   +E+++  +   EYD   F+ A+++PI++ LRERSL I L+ K E  +         +P IK+VWKW+   E++    +    +  T  F  ++ + Y +D+DE  ++ +MC   +  R ++  KY  G+ +R+ +E ++ +   + F + Y  PP+ P                      + +   L QTPW I G +K++TS++E++ +   K  K D  KF SSGREDVD R L SGRPFA E +NPR T  S   L+ +   IN+ST+ V I  +L  + +  LKTLKEGED KTKTYR++ +  S    TLDVLN       + + QKTPVRVLHRR  A R RT+  +K      KE  N   R                           FI  L  +    VKEFVHGDF RT PS+  I+  ++DI ALDV +INL+WP
Sbjct:   21 CIRCSLRLTGVRSPSHYENPVHSPVTLGYTNSSVSEVKNNYPCIACLGILDDETLTAAIEKIAAEVNNQEYDCDTFTCALTIPIAVQLRERSLQINLSRKFEFSKEAAAGFKRTVPQIKEVWKWIVLPEVEKLIDKTGVPISATCPFVIDVFLAYPQDEDECKALLKMCGNTFKKRLQQKKKYSDGVFSRKSVETAMINTLENQFVEHYPCPPSHPLSKTFINEVTCAHSSFFVGGRYNKYSRELSQTPWFICGEKKMETSVQELLCDTIVKFVKADSMKFLSSGREDVDVRTLHSGRPFAVELLNPRITKLSEAILQDLTQKINTSTELVHIVGNLQSISRDKLKTLKEGEDTKTKTYRALCLCRSQPLPTLDVLN---EIRDLEVIQKTPVRVLHRRPLATRLRTIHAMKASWISSKETINLQCRDQDSNKNPLIKVEDELLEKSNDLATNLFILDLTTQAGTYVKEFVHGDFGRTKPSICEILQIEVDIVALDVTSINLNWP 522          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|646690461|gb|KDR06864.1| (Putative tRNA pseudouridine synthase Pus10 [Zootermopsis nevadensis])

HSP 1 Score: 281.952 bits (720), Expect = 5.482e-86
Identity = 178/508 (35.04%), Postives = 276/508 (54.33%), Query Frame = 0

Query:    3 LSYKESVFQALEEAQDICFRCKLRYSGLREVYKYRTSEA-------------QNGSEGKISKANPCSACIGVLQDHFMLP-CLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEK------IEKFRSDD-IIPIKQVWKW-LFPTELKAEKKYLLGGT-SDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSY--------------SVPPNIPSLFESN--------LPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVE-MTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLK---FEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIG--CDIDIEALDVQAINLDWPP 457
            +S   +V++ L  +   C RC LRY G R+   Y   EA              +G E K  K NPC  C+G+LQ+   +   ++++   +  + YD  +F+ A+SLPIS  LR  SL + L +K      +++ R +  +I +K+VWKW + P   KA  K +  G  SDFF  + + Y+ D+DE + +  M  + + +R+ +  KYH  L TR+ ++ +LQ+   + F+  +              ++     SLF +         L QTPW+IDG R++++S++EII +  +K  + +  KF +SGREDVD R LG GRPF  E +NP +  F+ E+++S++  IN ++++V +  L ++ K  L  LK GE++K K Y ++ +    ++  +L+ L        +TLSQKTPVRVLHRR  A R RTV  +K    E   K   N +   F   +K +    +KEFVHGDF RT PSL  ++G  C +DI ALDVQ I LDWPP
Sbjct:    7 ISESHAVYEYLR-SLGCCQRCCLRYLGERDSNSYSCVEATLMKAGMEKERDCDSGPERKFFKYNPCIVCLGLLQEELSVDDVVQKIVDGVLKASYDCDNFTCALSLPISFQLRAHSLWLHLVDKFPLSFELQELRQETYVISLKEVWKWTVAPLIAKAINKEIDSGMKSDFFVGVNMVYSGDEDECSCLLEMHADIFMIRKTQKRKYHGNLYTRKAVDLALQTTEAAHFKQFFPSPPPIPTSSVTFDNITCTHNSLFIAGRYNKFSRTLSQTPWVIDGERRMESSVQEIISDSLQKASRAEGIKFSASGREDVDVRTLGRGRPFVCELLNPHRIKFTAEQVQSLQTDINKNSKEVSVRDLQIIQKDQLMNLKAGEERKIKHYLALCICLDAIKPGSLEQLGS---IKDLTLSQKTPVRVLHRRPLATRSRTVHSMKAWLLENPTKLCKNENVTMFKLDIKTQAGTYIKEFVHGDFGRTQPSLGQLLGGVC-VDILALDVQDIELDWPP 509          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|942317071|gb|JAN40468.1| (tRNA pseudouridine synthase Pus10 [Daphnia magna])

HSP 1 Score: 281.567 bits (719), Expect = 1.093e-85
Identity = 174/503 (34.59%), Postives = 265/503 (52.68%), Query Frame = 0

Query:   20 CFRCKLRYSGLR--EVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPC-LEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKI-----EKFRSDDIIPIKQVWKWLFPTEL-KAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMR----------------------------EKKSNKYHM------GLVTRQGIEKSLQSVSNSDFRDSYSVP-PNIPSL-------------------FESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPP 457
            C+RC +R++  R  E Y+ +  E +   E K+ KA+PC  C+G++QDHF+    ++EV Q +   + DS  FS  +SLP+++ LRE+++   LTEK      +  + D +  +K VWK++F   L K   K L   +S F   + I Y ++  E  ++  +C E++  R                            EK  ++Y+       G+ TR   +  L  +S  DF+  Y++  PN+ +                    F   L QTPW+I+G + +DTSI+E+I +  +  FK    KF +SGREDVD R LG GRPFA E  +P +TIF+ +++  ++  IN+ST  ++I  L  + K  L  LK+GE++KTK YR++ V       T + L      +++ L QKTP+RVLHRR  A+R RTV ++K       L +     FI  LK +    +KEFVHGDF RT P+L  ++G  IDI ALDV A+ LDWPP
Sbjct:   26 CWRCAIRFTRERKPEAYQRKLVEFKLEPEEKLLKASPCVICLGLVQDHFIKEVFIDEVCQQVLKFDCDSATFSCMVSLPVAIMLREQAVWFLLTEKFPDLLSKNLKIDYVTSLKDVWKFVFTEALEKKLNKQLEHSSSPFQIHISILYKDEISECGALVDICPEKFKHRYNRTRVYREGXXXXXXXXXXXXLVDICPEKFKHRYNRTRVYREGIFTRNAAQSVLSGLSAEDFQKYYTLKLPNVSAHYGPVQCSHNSIHIAGRYQKFSRKLCQTPWLIEGEKHMDTSIQELICKHLEPFFKTQSVKFSASGREDVDVRTLGRGRPFAIELTDPHRTIFTQDQITQLQNVINNSTDAIQIRDLQAVTKEQLSVLKQGEEEKTKIYRALCVTLDGSTPTEEDLAKINDITELVLMQKTPLRVLHRRPLAVRPRTVHEMK-----ATLLDDRPGHFILRLKTQAGTYIKEFVHGDFNRTVPNLCILLGRAIDIVALDVDAVELDWPP 523          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|242005765|ref|XP_002423732.1| (conserved hypothetical protein [Pediculus humanus corporis] >gi|212506923|gb|EEB10994.1| conserved hypothetical protein [Pediculus humanus corporis])

HSP 1 Score: 279.641 bits (714), Expect = 3.790e-85
Identity = 182/500 (36.40%), Postives = 252/500 (50.40%), Query Frame = 0

Query:   17 QDICFRCKLRYSGLREVYKYRTSEAQNGSEGKI-----------------SKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSDDIIP-----------IKQVWKWLFPTELKA--EKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLF----------------------ESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSD----VEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLK--QEPTVKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPPG 458
             ++C  C LRY  L E    + S + N  +G+I                 +K NPCSAC+G+LQ       L  V  S+  S YD  D + A+SLPIS  +R RS+ + L  K ++F  +   P           IK+VWKWL   ++     K    G   +F   + I Y +D+ E  S+ +M  EE+  R     K+H  ++TR+ +EK L       F + Y VPP  P+ F                        NL QTPW+I+G R++++S+EEII +P     K    KF SSGREDVD R LG+GRPF  E +NPRK     + +  +E  IN ST DV +  L ++ K DL  +K GE+ KTKTY    +I  D    +    + LN       + ++Q+TP+RVLHRR  A+R RTV  LK  A   E+ N +   F  SLK      +KEFVHGDF RTTP+L  I+  + DI ALDV+ + L WPP 
Sbjct:   18 NNLCNYCILRY--LNE----KNSNSYNDVDGRIKKLLNAQDEDYLPEIKKTKLNPCSACLGLLQLSDSSEQLNTVINSVIESGYDCDDVTFALSLPISFLIRSRSIWLHLINKFKEFFENKYYPHEIKQEYLVVSIKEVWKWLSSQKIAKMIGKNANNGVDGNFHVNIHINYDDDNLETASLFKMFPEEFQYRNHHKRKFHGEILTRKNVEKMLLETDLKSFDNIYPVPPKTPNTFFQFSTVQCMHDSIYVAGRYNKLSRNLSQTPWMINGERRMESSVEEIIEKPISNYIKNSGTKFSSSGREDVDVRTLGNGRPFVVEILNPRKVKIDQQYMLEIEKEINKSTNDVFVRDLQIVQKKDLHHIKAGEETKTKTYTCFCLIKLDNNDEIPEIKEKLNNLSQIKDLVINQRTPIRVLHRRPLAVRPRTVYWLK--ADFHEIKN-NHLFFKLSLKVGAGTYIKEFVHGDFGRTTPNLSEILEKETDILALDVEEVALQWPPN 508          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|942407683|gb|JAN85771.1| (tRNA pseudouridine synthase Pus10 [Daphnia magna])

HSP 1 Score: 279.256 bits (713), Expect = 1.577e-84
Identity = 176/523 (33.65%), Postives = 265/523 (50.67%), Query Frame = 0

Query:   20 CFRCKLRYSGLR--EVYKYRTSEAQNGSEGKISKANPCSACIGVLQDHFMLPC-LEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKI-----EKFRSDDIIPIKQVWKWLFPTEL-KAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVP-PNIPSL-------------------FESNLPQTPWIIDGVRKLDTSIEEII---IEPFKK---------------------------------------------------IFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWPP 457
            C+RC +R++  R  E Y+ +  E +   E K+ KA+PC  C+G++QDHF+    ++EV Q +   + DS  FS  +SLP+++ LRE+++   LTEK      +  + D +  +K VWK++F   L K   K L   +S F   + I Y ++  E  ++  +C E++  R  ++  Y  G+ TR   +  L  +S  DF+  Y++  PN+ +                    F   L QTPW+I+G + +DTSI+E+I   +EPF K                                                    FK    KF +SGREDVD R LG GRPFA E  +P +TIF+ +++  ++  IN+ST  ++I  L  + K  L  LK+GE++KTK YR++ V       T + L      +++ L QKTP+RVLHRR  A+R RTV ++K       L +     FI  LK +    +KEFVHGDF RT P+L  ++G  IDI ALDV A+ LDWPP
Sbjct:   26 CWRCAIRFTRERKPEAYQRKLVEFKLEPEEKLLKASPCVICLGLVQDHFIKEVFIDEVCQQVLKFDCDSATFSCMVSLPVAIMLREQAVWFLLTEKFPDLLSKNLKIDYVTSLKDVWKFVFTEALEKKLNKQLEHSSSPFQIHISILYKDEISECGALVDICPEKFKHRYNRTRVYREGIFTRNAAQSVLSGLSAEDFQKYYTLKLPNVSAHYGPVQCSHNSIHIAGRYQKFSRKLCQTPWLIEGEKHMDTSIQELICKHLEPFFKTQSILHINFTKILIIFLVMLRVIKGVKFSASGREDVDVRSIQELICKHLEPFFKTQSVKFSASGREDVDVRTLGRGRPFAIELTDPHRTIFTQDQITQLQNVINNSTDAIQIRDLQAVTKEQLSVLKQGEEEKTKIYRALCVTLDGSTPTEEDLAKINDITELVLMQKTPLRVLHRRPLAVRPRTVHEMK-----ATLLDDRPGHFILRLKTQAGTYIKEFVHGDFNRTVPNLCILLGRAIDIVALDVDAVELDWPP 543          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|1058057693|gb|JAS32508.1| (hypothetical protein g.8610 [Clastoptera arizonana])

HSP 1 Score: 274.633 bits (701), Expect = 3.580e-83
Identity = 173/487 (35.52%), Postives = 259/487 (53.18%), Query Frame = 0

Query:    8 SVFQALEEAQDICFRCKLRYSGLREVYKYRTSEAQNGSE--------GKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKI----EKFRSDDII--PIKQVWKWLFPTELKAEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIP--------------SLFESN--------LPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPKELSNGSDRRFIFSLKQEPTVKEFVHGDFKRTTPSLKSIIG-CDIDIEALDVQAINLDWPP 457
            S++  L+     C  C LR+ G  E   Y        ++         KI K NPCSAC+  L+  F+   +++V +++    YDS+ F+IA++LPIS  LR  S   FL        E F  + ++   +K+ WK++   +++          SD    + + Y+ D DE  S+ +M    +  ++KK NK    + +R+ +E +L+  +    R  Y +PP IP              S+F +         L QTPW++DG R+L++S++EII  P +K+ K +  KF SSGREDVD RMLG+GRPFA E +NP KTIF+ EELR VE  +NS  +DV+I  L  + K  L  LK GE+ KTK Y S  +  +     +  L    P   +TL Q+TPVRVLHRR  A+R+R +  +   +  K   +    + + + +    VKEFVHGDF RTTP+L  ++G   +DI ALDV+ I L+WPP
Sbjct:   22 SLYATLQNT-GCCKVCTLRFLGENEPQNYINPYGALKNKFVFPVEHAPKILKPNPCSACLDTLKPSFIDDTVDKVIEALNKENYDSETFNIALTLPISFYLRAHSFWFFLVSNFPFFCEVFHHNGVVVTGVKEAWKYIVIPKIEKSVNKQYNPISDLTISVELSYSSDYDESLSLLKMHPNIFGPQQKK-NKEKKIIFSRKSVEPALKQTNIELLRKFYEIPPPIPITSATCKGITVQHNSIFFAGRYNKYSRTLSQTPWVLDGKRRLESSVQEIIAAPLEKLSKAEGNKFISSGREDVDVRMLGNGRPFALELINPHKTIFTFEELRHVEKMLNS--KDVKIRDLQHIFKESLAMLKVGEESKTKNYNSYCIAWNVTPEQIHSLTTTKP---LTLYQRTPVRVLHRRPAAVRERIIYWMT-ASVEKICEDTIYFKLLINTQAGTYVKEFVHGDFGRTTPNLGELLGNAKVDILALDVEEIGLNWPP 500          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|954578121|ref|XP_014613553.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Polistes canadensis])

HSP 1 Score: 269.24 bits (687), Expect = 4.556e-81
Identity = 177/505 (35.05%), Postives = 259/505 (51.29%), Query Frame = 0

Query:    9 VFQALEEAQDICFRCKLRYSGLREVYKYRTSE-----AQNGSEGKISKAN---PCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKIEKFRSD------DIIPIKQVWKWLFPTELK--AEKKYLLGGTSDFFAELRIKYAEDDDEMNSISRMCKEEY-----TMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLF----------------------ESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVI---PSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFE-AFPKELSN-------GSDRRFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            +F  L   +  C RC LR++G      Y+  +      Q+  E ++       PC AC+G+LQ       ++ + Q ++   YD   F+ A+++PISL LRERSL I++ +K+   + +        + +K +WKW+   +L+    K+  L   S F  E+   Y  D++E  ++       Y       R  +  KY     +R+ IE  + S+++  F+  Y  PP  P  F                         L QTPW IDG +K++TS+++II EP  K  K +  KF SSGREDVD R + SGRPFA E +NPR T  S E L S+   IN S+  V+I + L LL + DLK LKEGE+ KTK YR++ V    P D +  +++LN       V + QKTP+RVLHRR    R R + D++   A P+EL N         +  F+  ++ +    VKEFVHGDF RT PSL  ++  ++DI ALDV  INLDWP
Sbjct:   12 IFHVLR-LEKCCLRCCLRFTGCNNPKYYQDLDELKNLIQSSEEKQVCVKETDLPCIACLGILQSKIEESVIKRIKQKVQNENYDCSTFTCALTVPISLFLRERSLHIYVAKKLNLTKDEYSTLKYKTLNVKDIWKWIALPKLEDIIGKRPDLTTPSPFLLEIFFLYVNDENECYTLLDQLGNNYDECSKQERMNRKRKYGNNKCSRKNIESVMISITDEMFQKYYLFPPTQPDSFINIEKVLCSHSSIFIGGRYNKLSRQLCQTPWFIDGEKKMETSVQDIICEPIVKAVKAESIKFQSSGREDVDVRNIHSGRPFAIELINPRITKISTELLSSLVTIINDSSTQVKITTNLKLLTRDDLKKLKEGENVKTKFYRALCVCRNAPQD-KSNIELLN---ELKNVEIIQKTPIRVLHRRPLTPRVRYIYDMRARWAKPQELLNLPNAPTDTDNMFFVLDVRAQAGTYVKEFVHGDFGRTKPSLCDLLNTEVDIVALDVTGINLDWP 511          

BLAST of EMLSAG00000000629 vs. nr
Match: gi|1000727253|ref|XP_015586236.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X3 [Cephus cinctus])

HSP 1 Score: 268.855 bits (686), Expect = 5.618e-81
Identity = 172/489 (35.17%), Postives = 256/489 (52.35%), Query Frame = 0

Query:   20 CFRCKLRYSGLR--EVYKYRTSEAQ-----NGSEGKISKANPCSACIGVLQDHFMLPCLEEVSQSIKTSEYDSKDFSIAISLPISLSLRERSLTIFLTEKI------EKFRSDDIIPIKQVWKW-LFPTELKAEKKYLLGGT--SDFFAELRIKYAEDDDEMNSISRMCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIPSLF----------------------ESNLPQTPWIIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINS-LSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTLDVLNGKLPASKVTLSQKTPVRVLHRRSNALRKRTVSDLKFEAFPK-ELSNGSDR----------RFIFSLKQEPT--VKEFVHGDFKRTTPSLKSIIGCDIDIEALDVQAINLDWP 456
            C RC LR+ G+R  E Y+   S  +     N +E  I +  PC  C+G+LQD      +E+V   +K  ++D   F+ A+++PI++ LRERS+ I L  +              +  +K +WK+   P   +A  K+    T  + F  ++   Y+ D++E   +  +C + + +R  +  KY  G+ +R+ +E ++ + S+ +F   +  PP++PS F                        +L QTPW I+G +K+ TS++E++ E   +  + D  KF SSGREDVD R L SGRPFA E +NP+ T  +   L  +   IN ST  V+I+S L +L K DLK LKEGED KTK YR++ +     +  LD LN       + + QKTPVRVLHRR    R RT+  ++       EL N   R           FI  +K +    VKEFVHGDF RT PS+  ++G DIDI ALDV +INL+WP
Sbjct:   22 CPRCCLRFVGIRTPEYYQNVISTLKHLGYINENESVIQEL-PCVICLGILQDSITDIAVEKVVSEVKKEDHDCPTFTCALTIPIAVQLRERSVQICLGHEFGLSESSLSSLKLRVQSVKDIWKYTAIPLIERATGKHADSVTINASFIIDIFFTYSNDENECKLLLNLCGKTFHLRAVQKRKYSDGVYSRKSVEAAMLTKSDEEFSKHFISPPSLPSRFVVVEKAICNHISIYIGGRYNKFSRDLSQTPWFINGEKKVGTSVQELLCEKIVEFVRADSIKFLSSGREDVDVRTLHSGRPFAIELINPKVTNITTTNLSDLVAKINESTDLVKIDSELKILNKMDLKKLKEGEDSKTKLYRALCICHETPKNPLDSLNN---VRNLKIIQKTPVRVLHRRPLTPRTRTIHAMQARWLKAVELENIMSRCKVPNSKDSSLFILDVKTQAGTYVKEFVHGDFGRTKPSICDLLGVDIDIVALDVTSINLEWP 506          

BLAST of EMLSAG00000000629 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold899_size83673-snap-gene-0.18 (protein:Tk06728 transcript:maker-scaffold899_size83673-snap-gene-0.18-mRNA-1 annotation:"hypothetical protein FLJ32312 ")

HSP 1 Score: 151.754 bits (382), Expect = 6.523e-44
Identity = 79/160 (49.38%), Postives = 108/160 (67.50%), Query Frame = 0

Query:  232 IIDGVRKLDTSIEEIIIEPFKKIFKFDDAKFYSSGREDVDTRMLGSGRPFAFEFVNPRKTIFSHEELRSVEXAINSSTQDVRINSLSLLLKSDLKTLKEGEDQKTKTYRSVVVIPSDVEMTL--DVLNGKLPASK-VTLSQKTPVRVLHRRSNALRKRTV 388
            ++DG RK ++S+EE+++   K    FD   F SSGREDVD RMLG GRPF FE VNPRKT+F  EE+  ++  IN+ +  V++  L ++ KSD+  LKEGEDQK KTYR++  I      T+  D +  +L   K + LSQ+TP+RVLHRR NA+R+RTV
Sbjct:    1 LVDGERKAESSVEELLMAEVKPSMVFDSFNFSSSGREDVDVRMLGMGRPFMFELVNPRKTMFKPEEMADIQCRINAHSTLVQVRDLQIVSKSDIVKLKEGEDQKKKTYRALCAITPAPGQTISWDKVKEQLEGIKDLELSQRTPIRVLHRRPNAIRRRTV 160          

BLAST of EMLSAG00000000629 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold899_size83673-snap-gene-0.19 (protein:Tk06729 transcript:maker-scaffold899_size83673-snap-gene-0.19-mRNA-1 annotation:"lactate 2-monooxygenase")

HSP 1 Score: 71.2478 bits (173), Expect = 1.442e-15
Identity = 30/49 (61.22%), Postives = 37/49 (75.51%), Query Frame = 0

Query:  171 MCKEEYTMREKKSNKYHMGLVTRQGIEKSLQSVSNSDFRDSYSVPPNIP 219
            MC+ EY+ R+K++  YHMGLVTR G+EKSL  VS+  FR  Y VPPNIP
Sbjct:   35 MCQSEYSKRQKQARTYHMGLVTRVGLEKSLSDVSDEKFRKHYPVPPNIP 83