EMLSAG00000006453, EMLSAG00000006453-689219 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000006453
Unique NameEMLSAG00000006453-689219
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592955346|gb|GAXK01003210.1| (TSA: Calanus finmarchicus comp187791_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 136.346 bits (342), Expect = 6.161e-35
Identity = 65/130 (50.00%), Postives = 86/130 (66.15%), Query Frame = 0 
Query:    4 AFKLVPLLSSILVFVASSPKQKGFCWEPGSNPFVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTDGEDFVFQIIAREDKRPKYGIEYKYSQMVTSYF 133
            A+ ++PLL +         +   FCW+P  NPF G P TER+DS  VR+ W  +F K   C++V+FLIKS+P  +PS+YKLSD T KG+ SAT+ V  GEDF+FQ+IARE+K    GIEY YS+  TS F
Sbjct:  882 AWVVIPLLCT---------QVSTFCWKPNHNPFTGAPDTERVDSTTVRVTWENVFPKGRGCEDVEFLIKSHPLDTPSNYKLSDLTLKGKRSATMAVPQGEDFIFQVIARENKGTGIGIEYAYSRPATSVF 1244

HSP 2 Score: 114.005 bits (284), Expect = 2.889e-27
Identity = 64/155 (41.29%), Postives = 92/155 (59.35%), Query Frame = 0 
Query:  199 EFIGYMNKNSVRYVHEFMRDKKAC----SEQSVGFIVSSPKTSTVKCCSSPWGGN-----CYIEFAECDGSCIPQDWISDNWPDCLDGSDEPXXXXXXXXXXXXXXQCVSCGXNXXXAVSLCSDKNYI--HLT-NCVKELLGDGNCSLCISDYFE 341
            +FI +MNKN++  V  F+ +KK+C    S Q  G +V        KCCS+PWG N     CY +FA CDG CIP DW+SD WPDC+DG+DE    ++ G+ +  +L CV C      A  LC + + +   LT NCV+ +LG G C++CI ++ +
Sbjct:   84 QFIKHMNKNALATVKTFVGEKKSCAGGYSGQEAGHLVEG--EGETKCCSTPWGDNSPQAVCYEQFARCDGRCIPHDWVSDGWPDCMDGADE-ATLTIDGKVMPYQLGCVHCAGVVLSAAFLCIESSSLTSGLTRNCVENILGPGQCNVCIQEFMD 539
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592791449|gb|GAXK01163119.1| (TSA: Calanus finmarchicus comp3623268_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 56.9954 bits (136), Expect = 1.575e-8
Identity = 36/116 (31.03%), Postives = 64/116 (55.17%), Query Frame = 0 
Query:    2 YSAFKLVPLLSSILVFVASSPKQKGFCWEPGSNP-FVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLM-VTDGEDFVFQIIAREDK 115
            +S    + L+S IL+ +ASS     +C   G+NP + G PK E++    V+++W  +  +  +C +   ++K    H+ +DY L+D       + T+  +T  E++ FQ+IAREDK
Sbjct:   32 WSLIHQLALISHILLPLASS-----YCSGVGNNPHWSGAPKIEQVTLTSVKVSWHGLLQRE-DCAD-QLIVKHWKTHNANDYSLTDLLSVETNNYTVENLTTMEEYTFQVIAREDK 358
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592759133|gb|GAXK01195280.1| (TSA: Calanus finmarchicus comp89200_c2_seq1 transcribed RNA sequence)

HSP 1 Score: 56.9954 bits (136), Expect = 2.490e-8
Identity = 44/134 (32.84%), Postives = 75/134 (55.97%), Query Frame = 0 
Query:   10 LLSSILVFVASS--PKQKGFCWEPGSNP-FVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTD---GEDFVFQIIAREDKRPKYGIEYK-YSQMVTSYFRED 136
            +L+ I+  +A++  P Q  +C   G+NP F   P  +++    V+++W  +  +   C +  F++KS  R +P+DYK+SD     Q S   +VTD    +D+VFQ +AREDK    GI  K +++   +YFR +
Sbjct:  497 MLNIIVTLLAATLQPAQ-AYCAGIGANPGFKDAPLVQQVTLTSVKVSWHGLVTR-AECAD-QFIVKSWNRRNPNDYKMSDLLPLNQFS--YIVTDLVPNQDYVFQAVAREDK----GILGKDWNKSPKAYFRTN 871
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592759135|gb|GAXK01195278.1| (TSA: Calanus finmarchicus comp89200_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 56.6102 bits (135), Expect = 4.230e-8
Identity = 42/133 (31.58%), Postives = 75/133 (56.39%), Query Frame = 0 
Query:   10 LLSSILVFVASSPK-QKGFCWEPGSNP-FVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTD---GEDFVFQIIAREDKRPKYGIEYK-YSQMVTSYFRED 136
            +L+ I+  +A++ +  + +C   G+NP F   P  +++    V+++W  +  +   C +  F++KS  R +P+DYK+SD     Q S   +VTD    +D+VFQ +AREDK    GI  K +++   +YFR +
Sbjct:  483 MLNIIVTLLAATLRPAQAYCAGIGANPGFKDAPLVQQVTLTSVKVSWHGLVTR-AECAD-QFIVKSWNRRNPNDYKMSDLLPLNQFS--YIVTDLVPNQDYVFQAVAREDK----GILGKDWNKSPKAYFRTN 857
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592759136|gb|GAXK01195277.1| (TSA: Calanus finmarchicus comp89200_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 56.6102 bits (135), Expect = 4.865e-8
Identity = 42/133 (31.58%), Postives = 75/133 (56.39%), Query Frame = 0 
Query:   10 LLSSILVFVASSPK-QKGFCWEPGSNP-FVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTD---GEDFVFQIIAREDKRPKYGIEYK-YSQMVTSYFRED 136
            +L+ I+  +A++ +  + +C   G+NP F   P  +++    V+++W  +  +   C +  F++KS  R +P+DYK+SD     Q S   +VTD    +D+VFQ +AREDK    GI  K +++   +YFR +
Sbjct:  612 MLNIIVTLLAATLRPAQAYCAGIGANPGFKDAPLVQQVTLTSVKVSWHGLVTR-AECAD-QFIVKSWNRRNPNDYKMSDLLPLNQFS--YIVTDLVPNQDYVFQAVAREDK----GILGKDWNKSPKAYFRTN 986
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592847122|gb|GAXK01110422.1| (TSA: Calanus finmarchicus comp220589_c7_seq42 transcribed RNA sequence)

HSP 1 Score: 36.1946 bits (82), Expect = 3.461e-2
Identity = 20/30 (66.67%), Postives = 20/30 (66.67%), Query Frame = 0 
Query:  253 EFAECDGS-CIPQDWISDNWPDCLDGSDEP 281
            EFA  DGS CIP  W  D   DCLDGSDEP
Sbjct:  244 EFACADGSKCIPGRWFCDTSVDCLDGSDEP 333
[Close]
BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592847124|gb|GAXK01110420.1| (TSA: Calanus finmarchicus comp220589_c7_seq40 transcribed RNA sequence)

HSP 1 Score: 36.5798 bits (83), Expect = 3.618e-2
Identity = 20/30 (66.67%), Postives = 20/30 (66.67%), Query Frame = 0 
Query:  253 EFAECDGS-CIPQDWISDNWPDCLDGSDEP 281
            EFA  DGS CIP  W  D   DCLDGSDEP
Sbjct:  244 EFACADGSKCIPGRWFCDTSVDCLDGSDEP 333
[Close]
BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592759134|gb|GAXK01195279.1| (TSA: Calanus finmarchicus comp89200_c0_seq3 transcribed RNA sequence)

HSP 1 Score: 37.7354 bits (86), Expect = 3.658e-2
Identity = 21/78 (26.92%), Postives = 45/78 (57.69%), Query Frame = 0 
Query:   10 LLSSILVFVASSPK-QKGFCWEPGSNP-FVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLS 85
            +L+ I+  +A++ +  + +C   G+NP F   P  +++    V+++W  +  +   C +  F++KS  R +P+DYK+S
Sbjct:    1 MLNIIVTLLAATLRPAQAYCAGIGANPGFKDAPLVQQVTLTSVKVSWHGLVTR-AECAD-QFIVKSWNRRNPNDYKMS 228
[Close]
BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592847170|gb|GAXK01110374.1| (TSA: Calanus finmarchicus comp220589_c5_seq33 transcribed RNA sequence)

HSP 1 Score: 36.1946 bits (82), Expect = 3.694e-2
Identity = 20/30 (66.67%), Postives = 20/30 (66.67%), Query Frame = 0 
Query:  253 EFAECDGS-CIPQDWISDNWPDCLDGSDEP 281
            EFA  DGS CIP  W  D   DCLDGSDEP
Sbjct:   27 EFACADGSKCIPGRWFCDTSVDCLDGSDEP 116
[Close]
BLAST of EMLSAG00000006453 vs. C. finmarchicus
Match: gi|592847121|gb|GAXK01110423.1| (TSA: Calanus finmarchicus comp220589_c7_seq43 transcribed RNA sequence)

HSP 1 Score: 36.1946 bits (82), Expect = 4.075e-2
Identity = 20/30 (66.67%), Postives = 20/30 (66.67%), Query Frame = 0 
Query:  253 EFAECDGS-CIPQDWISDNWPDCLDGSDEP 281
            EFA  DGS CIP  W  D   DCLDGSDEP
Sbjct:  250 EFACADGSKCIPGRWFCDTSVDCLDGSDEP 339
[Close]
BLAST of EMLSAG00000006453 vs. L. salmonis peptides
Match: EMLSAP00000006453 (pep:novel supercontig:LSalAtl2s:LSalAtl2s3535:2974:4429:-1 gene:EMLSAG00000006453 transcript:EMLSAT00000006453 description:"augustus_masked-LSalAtl2s3535-processed-gene-0.0")

HSP 1 Score: 694.115 bits (1790), Expect = 0.000e+0
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0 
Query:    1 MYSAFKLVPLLSSILVFVASSPKQKGFCWEPGSNPFVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTDGEDFVFQIIAREDKRPKYGIEYKYSQMVTSYFREDTAPDIGFKEVLTTEKLRVTKIKKSSLMTTTTPKYSRNAAKDKCVLPLKKFNSNNILSNKLLKEFIGYMNKNSVRYVHEFMRDKKACSEQSVGFIVSSPKTSTVKCCSSPWGGNCYIEFAECDGSCIPQDWISDNWPDCLDGSDEPELESLSGRSLSEELQCVSCGXNXXXAVSLCSDKNYIHLTNCVKELLGDGNCSLCISDYFE 341
            MYSAFKLVPLLSSILVFVASSPKQKGFCWEPGSNPFVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTDGEDFVFQIIAREDKRPKYGIEYKYSQMVTSYFREDTAPDIGFKEVLTTEKLRVTKIKKSSLMTTTTPKYSRNAAKDKCVLPLKKFNSNNILSNKLLKEFIGYMNKNSVRYVHEFMRDKKACSEQSVGFIVSSPKTSTVKCCSSPWGGNCYIEFAECDGSCIPQDWISDNWPDCLDGSDEPELESLSGRSLSEELQCVSCGXNXXXAVSLCSDKNYIHLTNCVKELLGDGNCSLCISDYFE
Sbjct:    1 MYSAFKLVPLLSSILVFVASSPKQKGFCWEPGSNPFVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTDGEDFVFQIIAREDKRPKYGIEYKYSQMVTSYFREDTAPDIGFKEVLTTEKLRVTKIKKSSLMTTTTPKYSRNAAKDKCVLPLKKFNSNNILSNKLLKEFIGYMNKNSVRYVHEFMRDKKACSEQSVGFIVSSPKTSTVKCCSSPWGGNCYIEFAECDGSCIPQDWISDNWPDCLDGSDEPELESLSGRSLSEELQCVSCGXNXXXAVSLCSDKNYIHLTNCVKELLGDGNCSLCISDYFE 341
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BLAST of EMLSAG00000006453 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold25_size650667-processed-gene-5.22 (protein:Tk06721 transcript:snap_masked-scaffold25_size650667-processed-gene-5.22-mRNA-1 annotation:"hypothetical protein AaeL_AAEL007475")

HSP 1 Score: 247.284 bits (630), Expect = 1.291e-74
Identity = 141/378 (37.30%), Postives = 200/378 (52.91%), Query Frame = 0 
Query:   14 ILVFVASSPKQ--KGFCWEPGSNPFVGQPKTERLDSDRVRINWSEIFNKNGNCKNVDFLIKSNPRHSPSDYKLSDFTQKGQTSATLMVTDGEDFVFQIIAREDKRPKYGIEYKYSQMVTSYFREDTAPDIGFKEVLT---------TEKLRVTKIKKSSLMTTTTPKY-----------SRNAAK-------------DKCVLPLKKFN----SNNILSNKLLKEFIGYMNKNSVRYVHEFMRDKKACSEQS-VGFIVSSPKTSTVKCCSSPWGG----------NCYIEFAECDGSCIPQDWISDNWPDCLDGSDEPELESLSGRSLSEELQCVSCGXNXXXAVSLCSDKNYIHLTNCVKELLGDGNCSLCISDYFE 341
            + +F+A  P       CW+PGSNPF GQP+  R   D V++ WS+IF+   +C+ VDF+IKS+PR  P+ YKLSDFT K Q SAT+ V  G D+VFQIIARE+K  + GIEYKYS MVT+Y  +  + D      L          T ++R T     + M  TTPK            SR                 DKC+  L   N     +N+ +++++  FI ++N+ S   V +F+  +K C  Q   GF V+ P    V CCSSPW             C  ++  CDG CIPQ W++D WPDCLDGSDE E      R+L ++L C+ C      +++LCS+        CV++++G+G+C+ CI D+  
Sbjct:  620 VTIFIALIPASVLGNLCWKPGSNPFYGQPRATRTGLDYVKLEWSDIFDGGSDCQLVDFMIKSHPRFKPAQYKLSDFTLKSQRSATVKVERGVDYVFQIIARENKGAQLGIEYKYSPMVTNYAMKLGSSDRVVNAQLASSPRQSPPITHEIRTTPPTSPAPM--TTPKTFLVPITRALPSSRERTNDQGLTGPTLFSPLDKCIPALSASNGFGHGSNMKTDRIIVNFIRFLNRQSQVEVGQFLATRKGCDAQHKKGFEVTCPGRQAV-CCSSPWTSCSSDRLGESQACVSDYFTCDGHCIPQKWVNDGWPDCLDGSDETEGVDRMARTLPQDLGCLQCSSVVLASMALCSNSGSGVTRQCVEDMMGEGDCNSCIGDFLR 994
The following BLAST results are available for this feature:
BLAST of EMLSAG00000006453 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000006453 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592955346|gb|GAXK01003210.1|6.161e-3550.00TSA: Calanus finmarchicus comp187791_c0_seq1 trans... [more]
gi|592791449|gb|GAXK01163119.1|1.575e-831.03TSA: Calanus finmarchicus comp3623268_c0_seq1 tran... [more]
gi|592759133|gb|GAXK01195280.1|2.490e-832.84TSA: Calanus finmarchicus comp89200_c2_seq1 transc... [more]
gi|592759135|gb|GAXK01195278.1|4.230e-831.58TSA: Calanus finmarchicus comp89200_c0_seq2 transc... [more]
gi|592759136|gb|GAXK01195277.1|4.865e-831.58TSA: Calanus finmarchicus comp89200_c0_seq1 transc... [more]
gi|592847122|gb|GAXK01110422.1|3.461e-266.67TSA: Calanus finmarchicus comp220589_c7_seq42 tran... [more]
gi|592847124|gb|GAXK01110420.1|3.618e-266.67TSA: Calanus finmarchicus comp220589_c7_seq40 tran... [more]
gi|592759134|gb|GAXK01195279.1|3.658e-226.92TSA: Calanus finmarchicus comp89200_c0_seq3 transc... [more]
gi|592847170|gb|GAXK01110374.1|3.694e-266.67TSA: Calanus finmarchicus comp220589_c5_seq33 tran... [more]
gi|592847121|gb|GAXK01110423.1|4.075e-266.67TSA: Calanus finmarchicus comp220589_c7_seq43 tran... [more]

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BLAST of EMLSAG00000006453 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 1
Match NameE-valueIdentityDescription
EMLSAP000000064530.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s3535:2974... [more]
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BLAST of EMLSAG00000006453 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000006453 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000006453 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000006453 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 1
Match NameE-valueIdentityDescription
snap_masked-scaffold25_size650667-processed-gene-5.221.291e-7437.30protein:Tk06721 transcript:snap_masked-scaffold25_... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s3535supercontigLSalAtl2s3535:2974..4429 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionaugustus_masked-LSalAtl2s3535-processed-gene-0.0
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000006453 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000006453EMLSAT00000006453-702300Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s3535:2974..4429-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000006453-689219 ID=EMLSAG00000006453-689219|Name=EMLSAG00000006453|organism=Lepeophtheirus salmonis|type=gene|length=1456bp|location=Sequence derived from alignment at LSalAtl2s3535:2974..4429- (Lepeophtheirus salmonis)
ATGTATAGTGCATTTAAGCTGGTCCCACTTTTATCTTCAATACTAGTGTT
TGTTGCCTCAAGCCCCAAGCAAAAAGGTTTTTGCTGGGAGCCTGGAAGTA
ATCCATTTGTTGGTCAACCAAAAACTGAAAGACTTGACTCCGACAGAGTA
AGGATTAATTGGTCAGAAATTTTCAATAAGAATGGTAATTGTAAAAATGT
AGACTTCCTTATCAAATCAAATCCAAGACATAGCCCCTCTGACTACAAAC
TATCGGATTTTACACAAAAGGGACAGACCAGTGCAACTCTTATGGTAACG
GATGGAGAAGATTTTGTATTTCAAATTATAGCACGGGAAGACAAGAGACC
CAAATATGGTATTGAGTATAAATATTCACAGATGGTTACTTCATATTTCA
GAGAAGATACTGCCCCAGACATTGGGTAACTATCATTGTATTGTTTATGT
AATTGTACTTCATACTTTTTAACAAGTGAGAGGCACTGCTCCAACCTATG
TAGCAAATAATTACTAGCACATAAATGCAGAGAACTTTATTCCCTTGCTA
TTGTAAACAATAAAAATATTTCCATATTTGTGTCATAACCCTTGGAATTA
ATTTCAATTTACTTAAATATGTATGAAAATTTGTTGGAATGATAGTTTGA
AATAATAAAAATGTGACCGCAAATATTCTCATTTCAAAAATGTTTGAGTA
ACTACCCACTAGTTGTGTTATAATTTATTTTATAAAATAATGTTTAAGTA
AATTGATCATTTCAAGAAAAATCTACGCAATAAGTACATACTATATAATC
TTTGTATATATGGTAAAACTAAGTCTTTCACAAGTTCTGTTTTTCTCCAT
TCGAGGTTCAAGGAAGTCTTAACAACAGAAAAACTACGAGTCACAAAGAT
TAAAAAGTCATCATTAATGACAACTACTACGCCTAAATATTCACGCAATG
CCGCCAAGGATAAGTGTGTCCTACCCCTAAAGAAATTTAATTCGAATAAT
ATTCTATCGAACAAACTACTCAAGGAATTCATTGGATATATGAATAAAAA
CTCTGTTAGATATGTTCATGAGTTTATGCGAGACAAAAAAGCCTGTTCAG
AACAAAGTGTAGGGTTCATTGTCAGTTCCCCGAAGACAAGTACTGTTAAA
TGTTGTTCTAGCCCTTGGGGTGGTAATTGCTACATTGAATTCGCGGAATG
TGATGGATCCTGCATACCTCAAGATTGGATATCTGATAATTGGCCGGACT
GTTTGGATGGTAGTGATGAACCTGAATTAGAAAGCTTGAGTGGTAGATCC
TTGTCTGAGGAGCTACAGTGTGTTAGTTGTGGTGRAAATKTCWTTSCCGC
CGTTTCTCTTTGTAGTGATAAAAATTATATTCATTTGACCAATTGTGTAA
AAGAGCTTTTAGGTGATGGAAACTGTAGTTTGTGCATTTCTGATTATTTC
GAATAA
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