conserved hypothetical protein, maker-scaffold108_size357748-snap-gene-2.20 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000005066 (supercontig:LSalAtl2s:LSalAtl2s266:205294:402426:1 gene:EMLSAG00000005066 transcript:EMLSAT00000005066 description:"maker-LSalAtl2s266-augustus-gene-3.8") HSP 1 Score: 1060.44 bits (2741), Expect = 0.000e+0 Identity = 522/895 (58.32%), Postives = 672/895 (75.08%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGS-QVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISS-KYVEGFFVRFRDMSGGSQKFNMKTVM-KQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNV-TLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHPQD---VGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHWKNGSD---LVVQGQKSGECFYNDNSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLA-MQNRIRSVHGKTFMALPQ 890
L +DFR P DT +A+GD V+KC P+GNPKPSV W KNG +L + R+ +TE G+LVI++ VK+D+G CQA N+VG R + L + VKP F+ +P D+T+ G ++ +IC V+GDP+P ++W R+ D LP+ +D L I P D+G+Y C+A N VG V S ++VHA P KPKDQR+GMNGIAKFEC GNPPPS+FWTKEG+QELMF+GT +GQ VS+DGTL IQGVRKED GFY+CSALSVAGST KAYLEVTA+ D PPPII MG ANQTLP+NTIA+LPC+ASG PKP+I+W+KNQ P+ +SSRI++E+SGTLVIDNL DT LYTCTA SESGETSWSASLS DPKNPN+IFHKTPDPSTFP PPT+PKIV+RRSTS+TISWRR+ TG+SPL+GYT+EYFS ++ETGWVVAA RITSETYT+NNLKPD+SY+FLVRAENSHGMSPP+++SERVRTL++ + +D +N+ EV N L+ KVV+L SIEAISSTTIRVSW +L S YVEGFFVR+RDMSGGSQKFNMKTVM + D I ++VITNLRK+TEYEVFL PF+K IEGQPSNSLHVQT+ D PSAPP+ ++ E +N TSA I SPPPPQHRNG+L GYQ+HVKGNGSTFHSN+ TLNATT +ILSNL+L+++Y++RA A T+ GLGPFS+P F MDP IK +++HP + G+++SEPWF+ L+++ + + F+ I++YR++++ K SL V + YED++++ G++W+N + WK G+ + + + + F +++ LYAEVG+ NGF T + +P+PYATTTLA MQN++R ++G TF++LPQ
Sbjct: 125 LREDFRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNGDILDTSQSN-GRIIITEVGNLVIQEVVKSDEGQXACQAENIVGIRETEEAQLSIQVKPYFLSKPLDITAISGDDIDIICDVSGDPDPIVIWSRD-DGPLPENKVSIDANRLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISVHAKPAIQTKPKDQRIGMNGIAKFECSAGGNPPPSLFWTKEGSQELMFAGTKYGQRSVSEDGTLFIQGVRKEDEGFYICSALSVAGSTATKAYLEVTALRDTPPPIIIMGTANQTLPLNTIAILPCKASGAPKPTISWYKNQSPISIDDSSRILVESSGTLVIDNLISSDTGLYTCTASSESGETSWSASLSXXDPKNPNVIFHKTPDPSTFPDPPTRPKIVDRRSTSITISWRRSPNTGESPLMGYTLEYFSSDLETGWVVAANRITSETYTINNLKPDTSYMFLVRAENSHGMSPPTRVSERVRTLQSLS-ENDGINIHEVSNALMSKVVQLTSIEAISSTTIRVSWSILPESMSYVEGFFVRYRDMSGGSQKFNMKTVMLETDGIVNHVITNLRKYTEYEVFLMPFYKKIEGQPSNSLHVQTMEDVPSAPPSDLLIEPMNSTSAIIRWSPPPPQHRNGVLLGYQIHVKGNGSTFHSNITTLNATTTSYILSNLTLHQKYSVRAVARTKIGLGPFSQPSFFVMDPTNIKYAIITHPNEGNINGNIMSEPWFIGLLTAVFVAISGTFIGIIIYRKKFNSTK-SLSQNTVASRHYEDITKIAINPDGSRGTVWINDD-------WKCGTKSTGIYDEAETNQRYFRPSDNNNTDLYAEVGEP-------NGFSTFGGNHRITEPSPYATTTLAVMQNKVRKMNGNTFISLPQ 1001 HSP 2 Score: 145.976 bits (367), Expect = 9.957e-36 Identity = 135/443 (30.47%), Postives = 202/443 (45.60%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK--RVTLTETGSLVIRDAVKA----DQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQ-----VDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGT-LVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYA-VESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKN--PNIIFHKTPDPSTFPQPPTKPKIVNRRSTSV---TISWRRNHQTGQSPL 432
E+P + + + L C++ G P+P+++W K+G+++ PK RV L +GSL AV+ D G Y C+A N VGT S TL V V + F P D G + C G+P+P + W + D+ S + G LVI + DEG +C+A N VG + ++ L++ P FL KP D C V G+P P + W+++ + VS D L I+ VR ED G Y C A + G A + V A P I P +Q + +N IA C A GNP PS+ W K +Q+ ++A + + + GTL I +R ED Y C+A S +G T+ A L V ++ P II T + + P I+ +++ TISW +N QSP+
Sbjct: 33 EQPTSMTVPKNEPVTLNCKA-DGIPEPNITWYKDGELV---PTAPKTHRVIL-PSGSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLREDFRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNGDILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVGIRETEEAQLSIQVKPYFLSKPLDITAISGDDIDIICDVSGDPDPIVIWSRDDGP--------LPENKVSIDANRLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISVHA-----KPAIQTKPKDQRIGMNGIAKFECSAGGNPPPSLFWTKEGSQELMFAGTKYGQRSVSEDGTLFIQGVRKEDEGFYICSALSVAGSTATKAYLEVTALRDTPPPIIIMGTANQTL---PLNTIAILPCKASGAPKPTISWYKN----QSPI 450 HSP 3 Score: 115.161 bits (287), Expect = 3.463e-26 Identity = 88/290 (30.34%), Postives = 139/290 (47.93%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKGIL---------VIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG-STLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
P ++P +T VTL C G PEP++ W ++ +L +P + + IL + + HD G+Y CEATN VG S++V L V F + P D V I +C GNP PS+ WTK G +++ + ++G++ +++ G LVIQ V K D G C A ++ G +A L + Q P P + T + C SG+P P + W ++ PL +++ I+A+ L I ++R ED +Y C A ++ G S S+SV
Sbjct: 29 PQITEQPTSMTVPKNEPVTLNCKADGIPEPNITWYKDGEL-VPTAPKTHRVILPSGSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLREDFRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNG--DILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVGIRETEEAQLSI-----QVKPYFLSKPLDITAISGDDIDIICDVSGDPDPIVIWSRDDGPL---PENKVSIDAN-RLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISV 306 HSP 4 Score: 71.2478 bits (173), Expect = 1.063e-12 Identity = 61/203 (30.05%), Postives = 95/203 (46.80%), Query Frame = 0
Query: 189 VHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSG-TTHGQMHVSQDGTL----VIQGVRKEDGGFYVCSALSVAGSTLAK-AYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQ-ASGNPKPSINWFKNQQPLYAVESS-RIMIEASGTLVIDNLRMEDTALYTCTAGSESG-ETSWSASLSVE 382
+ PT + PK++ V +N C DG P P+I W K+G EL+ + TH + S G+L +Q ++ DGG Y C A + G+ ++ L+V + + + P + + I V+ C GNPKPS+ W KN L +S+ RI+I G LVI + D C A + G + A LS++
Sbjct: 31 ITEQPTSMTVPKNEPVTLN------CKADGIPEPNITWYKDG--ELVPTAPKTHRVILPS--GSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLRED----FRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNGDILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVGIRETEEAQLSIQ 219
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000004040 (supercontig:LSalAtl2s:LSalAtl2s215:590464:702398:-1 gene:EMLSAG00000004040 transcript:EMLSAT00000004040 description:"maker-LSalAtl2s215-snap-gene-7.10") HSP 1 Score: 344.739 bits (883), Expect = 6.430e-103 Identity = 264/892 (29.60%), Postives = 422/892 (47.31%), Query Frame = 0
Query: 15 PDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNG-KVLHELEDEPKRVTLTETGSLVIRDAV----KADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLV-AGDPEPHLVWRRENDLALPKGSQ-----VDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTV-MKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLS--LNEQYTIRASAFTQAGLGPFSRPVTFTMDP-------------AYIKSPVVSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNAN------SGHLHHHWKNGSDLVVQGQKSGECFYNDNSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLA 872
P+ T + + D L+C + G P P + W +NG K +D R L GSL V + D G Y C ARN +G+ S ++ F P D G LIC G+P P + W++ + + + D+G LVIP + P DEG Y C A N G K +Q + L V P+ P++ + + CV +G P P I W+K + G G L+I+ D Y C A + AG A A AI PPI + P+NQ + V A L C A+GNP P + W K + L + D+ LY C A S SG++ WS+SL V + I F PD S FP P+KP + N S S+T++W + H+ G SPL GY ++Y++ W + SE +T+++L P+++ LVRA N +G+SPPS +S+ ++TL S ++ +++ L +++ L+ ++ S I++ W++LIS ++VEG+ ++ R GS++ + +V + S+ +++LR T+Y +F+ PF I+G+PSN V T D PS+ P + ++N+++ I PP + NG L G++V + N S+ SN TL F+++ LS Y + +A+ + G+GPFS V+ ++P + SP + P ++ E WF+ F+S + +VL++ F A++ RR+ G ++GH VP+ + +D+S H +RD +W+ +N +G H + N L Q + N + GD EV T + K S GD PYATT+L
Sbjct: 39 PESTIVPKNDPVTLRCDT-SGXPLPEIIWRRNGRKXTTAADDHRSRRVLLPNGSLFFLRTVHSRKETDGGIYSCVARNTLGSVESL-------LRDKFRGEPVDSDVILGQTAELICXPPKGNPSPTVFWKKNGQILDLETEEERLYIKDEGTLVIPDSIPGDEGSYQCIAKNIAGTKATQRIHLEVGIVPSITKAPENASIASGKEIRLVCVAEGKPKPMISWSKSAGSLPLSRSRIEG-------GLLIIKNAITTDEDVYRCMAENSAGMDTATA-----AIRIHSPPIFLVKPSNQKIAVGAPASLDCIAAGNPTPVLFWMK-----------------------EGLSLSDSGLYXCQARSVSGQSVWSSSLVVSEGGGL-IDFKPMPDLSQFPGSPSKPSLANATSHSITVTWSKPHRVGGSPLKGYQLDYYTLASPLHWT-TVYNVHSEFHTIDDLDPETTLWVLVRALNGYGLSPPSPVSKSLKTLAPDPNSRTGMDPWSIRSKLGGRIMILIGAISVGSRKIKLEWELLISGEFVEGYHIQIR----GSKEPHYDSVTISGGGTRSHTLSDLRPNTKYFIFILPFNNRIKGRPSNLKVVSTKEDVPSSSPDRIKIRLVNVSTGLIQWHTPPSRDLNGDLSGFKVLIFVN-SSLESNYTLEPDVNSFLITELSSDFETAYGAKVAAYNRQGVGPFSPMVSININPMSGDIHGVHRNELSEFFSPEEASPHGFDIVLQEVWFVSFVSVMTLVLVIAFGAVICIRRR-KGELKNMGHYNVPIHKVDDIS--HVNFGNRD-HLWLXSNWKSSETNGKGKHIFIN--KLTNFTQNATNNSTNTMRSNSNRMDKDGDYAEVDTPKSTDTHQKDLVSKGD-GPYATTSLV 873 HSP 2 Score: 183.341 bits (464), Expect = 1.287e-47 Identity = 92/268 (34.33%), Postives = 141/268 (52.61%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG 274
L D FR EP D+ + G TA L C P+GNP P+V W KNG++L +LE E +R+ + + G+LVI D++ D+G Y C A+N+ GT+ + L V + P + PE+ + G E+ L+C+ G P+P + W + S+++ G+L+I A DE +Y C A N G + + + +H+ P FLVKP +Q++ + A +C+ GNP P +FW KE G+ D G Y C A SV+G
Sbjct: 124 LRDKFRGEPVDSDVILGQTAELICXPPKGNPSPTVFWKKNGQIL-DLETEEERLYIKDEGTLVIPDSIPGDEGSYQCIAKNIAGTKATQRIHLEVGIVPSITKAPENASIASGKEIRLVCVAEGKPKPMISWSKSAGSLPLSRSRIEGGLLIIKNAITTDEDVYRCMAENSAGMDTATAAIRIHSPPIFLVKPSNQKIAVGAPASLDCIAAGNPTPVLFWMKE--------------------------GLSLSDSGLYXCQARSVSG 364 HSP 3 Score: 111.309 bits (277), Expect = 5.041e-25 Identity = 86/295 (29.15%), Postives = 130/295 (44.07%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE-------------NDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLA-KAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
P+ + PE VTL C +G P P ++WRR + LP GS + + + D G+YSC A N +G V + F +P D V + A+ C GNP P++FW K G +++ T ++++ +GTLVI D G Y C A ++AG+ + +LEV + P I P N ++ L C A G PKP I+W K+ L SR IE G L+I N D +Y C A + +G + +A++ + P
Sbjct: 33 PLILSHPESTIVPKNDPVTLRCDTSGXPLPEIIWRRNGRKXTTAADDHRSRRVLLPNGSLF--FLRTVHSRKETDGGIYSCVARNTLGSVESLL------RDKFRGEPVDSDVILGQTAELICXPPKGNPSPTVFWKKNG--QILDLETEEERLYIKDEGTLVIPDSIPGDEGSYQCIAKNIAGTKATQRIHLEVGIV-----PSITKAPENASIASGKEIRLVCVAEGKPKPMISWSKSAGSL---PLSRSRIEG-GLLIIKNAITTDEDVYRCMAENSAGMDTATAAIRIHSP 308
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000005454 (supercontig:LSalAtl2s:LSalAtl2s293:185286:259880:1 gene:EMLSAG00000005454 transcript:EMLSAT00000005454 description:"maker-LSalAtl2s293-augustus-gene-2.25") HSP 1 Score: 180.259 bits (456), Expect = 3.391e-46 Identity = 184/726 (25.34%), Postives = 303/726 (41.74%), Query Frame = 0
Query: 22 QGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGS----LVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFI--QRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVD---------KGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKE----GNQELMFSGTTHGQMHVSQ-------DGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEAS-------GTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYF----SFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKY---VEGFFVRFRDMSGGSQK-FNMKTV---MKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFH---SNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLG 700
+G+ A L C G+ SV+W +G + +T T G LVI +G+Y C A N G +S L VN P + ++V S+G + + GD + W + G + IL+I E G Y+C A+N G S + L ++ P + V+P D+ AK +C DG P P I W K + E + + + + VS DGTL+I+ ++K + GFY+C A + G A A + V Q PP + QT ++ VL C+A G + W N + L A R I T+ I D+AL+TC A + G S +L +++ P+ P K++++ +V +SW+ + G S L Y +E+ S++ + V+ V +LKP +SY F + A+N G+S PS D + +E + V++ + A+ T+RVSWK ++ + + G++V ++ + +K + +TV +Q + I+NL FTEY V + F K +G S P+ P + L+ S ++T S PP +GI++GY+V + G ++ S+ T ++ M L+ L Y+I+ AFT G G
Sbjct: 1922 EGNFAQLTCVVTSGDLPLSVTWTFHGDKV----GTQTGITTTNLGPRLSLLVINSVGYRHRGNYTCXASNNAG-HDSYTAKLRVNEPPSLVPLNFGKNVFSEGSFAQVVCTITEGDEPIKISWSFHGESLEDSGQNIGIAITNIGSRTSILIINSVEHKHRGNYTCSASNKAGNSSDTAQLKINVRPRWTVEPTDKHFAQGSEAKIDCKSDGFPQPKIVWRKAIGLPVSNEQXPTPSDYKDLDVSNDRNMKIIDGTLIIKNIQKSNEGFYLCKASNGIGGISAVAKISV-----QAPPSFEIKKRTQTALISDNTVLQCEAKGEKPIGVLWNMNNKRLDAKVDDRYTIREEVIDDGVLSTISIKRTERGDSALFTCVATNAFGSDDTSINLIIQEN----------------PETPYDLKVLDKAGRTVKLSWQTPYN-GNSNLTRYVIEFKPTKGSWKNDIDHVLVPGD--QNQAVVYSLKPANSYHFRIVAQNVIGVSGPS----------------DTITIETAEEAPSGPPVDI-RVSAVDQQTLRVSWKPPLTEHWNGDILGYYVGYKATANSEEKPYLFETVEFRKEQGHEHQLQISNLEVFTEYAVVVQAFNKIGQGPMSXEXLXHXAEGAPTESPQDIDLVTLSSQSIKVTWSSPPLASAHGIIKGYKV-IYGPSKVWYDPSSHATKISSDMQAELTGLQRYTNYSIQVLAFTNGGDG 2600 HSP 2 Score: 124.405 bits (311), Expect = 7.613e-29 Identity = 159/738 (21.54%), Postives = 297/738 (40.24%), Query Frame = 0
Query: 20 IAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRV--TLTETGS----LVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRR------------------ENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQ-ELMFSGTTHGQMHVSQDG---TLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEAS-GTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWR---RNHQTGQSPLIGYTVEYFSF--EMETGWVVAALRITSET-----YTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWK---VLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYV---ITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKG---NGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFT 709
++G A + C G+ +SW +G+ LED + + +T GS L+I +G+Y C A N G +S L +NV+P + P D GSE + C G P+P +VWR+ + D++ + ++ G L+I + +EG Y C+A+N +G +S ++V A P+F +K + Q ++ +C G P + W + + + V DG T+ I+ + D + C A + GS L + + P + + A +T+ ++ P + N + FK + + + +++ V+ +L+ ++ + A + G + S ++++E + PS PP ++ ++ +SW+ H G ++GY V Y + E ++ + E ++NL+ + Y +V+A N G P S A S D++L +SS +I+V+W + + ++G+ V + G K I S + +T L+++T Y + + F +G + T D P PP++V A L M+ I +S P+ +NG + Y V++K + ++ +++ M + NLS +Y +A T G GP S+ VT T
Sbjct: 2016 FSEGSFAQVVCTITEGDEPIKISWSFHGE---SLEDSGQNIGIAITNIGSRTSILIINSVEHKHRGNYTCSASNKAG-NSSDTAQLKINVRPRWTVEPTDKHFAQGSEAKIDCKSDGFPQPKIVWRKAIGLPVSNEQXPTPSDYKDLDVSNDRNMKIIDGTLIIKNIQKSNEGFYLCKASNGIGGISAVAKISVQAPPSFEIKKRTQTALISDNTVLQCEAKGEKPIGVLWNMNNKRLDAKVDDRYTIREEVIDDGVLSTISIKRTERGDSALFTCVATNAFGSDDTSINLIIQENPETPYDLKVLDKAGRTVKLSWQT--PYNGNSNLTRYVIEFKPTKGSWKNDIDHVLVPGDQNQAVVYSLKPANSYHFRIVAQNVIGVSGPSDTITIETAEEA---------PS---GPPVDIRVSAVDQQTLRVSWKPPLTEHWNGD--ILGYYVGYKATANSEEKPYLFETVEFRKEQGHEHQLQISNLEVFTEYAVVVQAFNKIGQGPMSXEXLXHXAEGAPTESPQDIDLV-----------------TLSSQSIKVTWSSPPLASAHGIIKGYKVIY-----GPSKVWYDPSSHATKISSDMQAELTGLQRYTNYSIQVLAFTNGGDGTKTEVFTTTTEQDIP-GPPSSVKA--LAMSDDSILVSWQLPKEQNGKIIQYTVYIKELDRSRDIAPTSRKVDSLQMSLQIDNLSSKARYEFWVTAHTSIGGGPRSKKVTLT 2708 HSP 3 Score: 119.783 bits (299), Expect = 1.862e-27 Identity = 131/496 (26.41%), Postives = 213/496 (42.94%), Query Frame = 0
Query: 14 EPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVK-----PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVW-----RRENDLALPKGSQVDKG-----ILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGV-RKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP-SINWFKNQQPLYA---VESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKN--PNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPS 487
EP + G + KC +GNP V WLKNGK ++ E+ +L I K D+G Y C RN + S T + + P FI E+ K G +++ C+ GDP P++ W +N+ A+ GS D L I + + G Y C+A++ VGK+S L V P ++ K + +V C V G P +I W K+G Q L F + + V +GTL+I + RKED Y C A + G + A++ LEV+ +E P I+ + ++ A + C P I+W + + + +S++ AS L I ++ + YTCTA +++G S+S SL+V +P + P D F Q IV++ +T+SW T++ E G + + T+N++ S V+ ++N G++ S
Sbjct: 191 EPKTQVVDYGRSVTFKCNY-KGNPIKEVFWLKNGKSINHNEN-----------TLRINSVKKDDRGMYQCFIRN---EQESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLSISCIAKGDPSPNIKWFIYGKEVKNEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNVFGAP-YVKKMRPMKVVAGKSMFITCPVAGYPISNIVWEKDGRQ-LPF----NDRQTVFPNGTLIISDIQRKEDASVYTCVAXNDEGYS-ARSDLEVSVMER--PEIVPFFFGSDSVDEGLYAQVSCIVQRGDTPIKISWSLKGDTVSSEPGMTTSQVGPRAS-ILSIHSVGYRHSGTYTCTAQNKAGIISYSTSLTVNEPPSLMPLTFGRDILDEGEFAQLIC---IVSKGDEPLTMSW--------------TLKGDDVSSEPGLSTTKIGTRTSILTINSVGYRHSGVYTCTSQNEAGVATQS 644 HSP 4 Score: 100.138 bits (248), Expect = 1.862e-21 Identity = 109/417 (26.14%), Postives = 167/417 (40.05%), Query Frame = 0
Query: 22 QGDTAVLKCQSPRGNPKPSVSWLK-----NGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQ-RPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAH---PTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGT---LVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNL-RMEDTALYTCTAGSESGETSWSASLSVEDPKNPNII----FHKTPDPSTFPQPPTKPKIVNRRSTSVTISW 421
+G + L C + +P PS W K N K L +L+D K V G+L+I++A D G Y+C N VG + T+ P+ + P+ G VT C G+P + W + K ++ L I + D GMY C N + L + P F+ ++ V G C+ G+P P+I W G + T G + L I V+ + GG Y C A S G A L V P + M P + + C +G P +I W K+ + L + R + +GTL+I ++ R ED ++YTC A ++ G ++ S L V + P I+ + D + Q IV R T + ISW
Sbjct: 104 EGLSFALICPA-MASPIPSFRWYKFMEANNNKKLVKLDDRVKXVG----GTLIIKEAKVEDSGKYLCMVNNSVGGESV--ETVLTVTAPLSVDVEPKTQVVDYGRSVTFKCNYKGNPIKEVFWLKNG-----KSINHNENTLRINSVKKDDRGMYQCFIRNEQESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLSISCIAKGDPSPNIKWFIYGKEVKNEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNVFG----APYVKKMRPMK--VVAGKSMFITCPVAGYPISNIVWEKDGRQLPF--NDRQTVFPNGTLIISDIQRKEDASVYTCVAXNDEGYSARS-DLEVSVMERPEIVPFFFGSDSVDEGLYAQVSC---IVQRGDTPIKISW 496 HSP 5 Score: 95.9005 bits (237), Expect = 3.737e-20 Identity = 83/278 (29.86%), Postives = 114/278 (41.01%), Query Frame = 0
Query: 117 KGGSEVTLICLVAGDPEPHLVWRR----ENDLALPKGSQVDK---GILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWF------KNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
K G LIC P P W + N+ L K K G L+I +A+ D G Y C N VG S VLTV A + V+PK Q V F+C GNP +FW K G H + TL I V+K+D G Y C + S A A L++ D PP I+ P +++ C A G+P P+I WF KN+ + + + L I ++ + Y C A S+ G+ S A L+V
Sbjct: 103 KEGLSFALICPAMASPIPSFRWYKFMEANNNKKLVKLDDRVKXVGGTLIIKEAKVEDSGKYLCMVNNSVGGESVETVLTVTAPLSVDVEPKTQVVDYGRSVTFKCNYKGNPIKEVFWLKNGKS------INH------NENTLRINSVKKDDRGMYQCFIRNEQESAQATAELKLGGRFD-PPEFISHFEETIVKPGGYLSI-SCIAKGDPSPNIKWFIYGKEVKNEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNV 366 HSP 6 Score: 84.3445 bits (207), Expect = 1.459e-16 Identity = 97/382 (25.39%), Postives = 154/382 (40.31%), Query Frame = 0
Query: 22 QGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTS-KGGSEVTLICLVAGDPEP-HLVWRRENDL--ALPKGSQVDKG----ILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFL-VKPKDQRVGMNGIAKFECVVD-GNPPPSIFWTKEGN---QELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP-SINW-----FKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
+G+ A + C RG+ S++W G V+ + + L I+ G Y CQA+N G+ +L VN P + S K G L CLV EP + W + D+ + P + G +L+I G Y+C AT G + S L V+ P+ L V I + C+V G+ P I W+ +G+ E + T G ++ L+I V G Y C A + AG A L V ++ P I+A + ++ + L C S +P +I W + +P ++ ++ I S L+I N+ + YTC A + G + SA L V +P
Sbjct: 849 EGEFAQIVCIVSRGDEPLSITWSLYGAVISSEPSISTNLLGSRXSMLXIQSVGYRHNGIYTCQAKNEAGSMRQS-ASLIVNESPSILPFSFGAESVKEGDINQLTCLVNRGDEPLTITWSLKGDVVSSXPSITTTMIGTRASMLMITNVGYRHSGTYTCRATXSAGXATHSAELKVNESPSILPFSFGSNNVKEGDITQLTCLVSRGDEPVRITWSLKGDVISSEPSITTTMFG----TRTSLLMITNVGYRHSGXYTCRASNAAGXATHSAELNV----NESPSILAFSFGSDSVKEGDMTQLTCFVSRGDEPLTITWSLKGDVVSSEP--SITTTMIGTRVS-MLMITNVGYRHSGTYTCRATNSGGTATHSAELKVNEP 1218 HSP 7 Score: 83.5741 bits (205), Expect = 2.099e-16 Identity = 103/391 (26.34%), Postives = 156/391 (39.90%), Query Frame = 0
Query: 20 IAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTL---TETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTS-KGGSEVTLICLVAGDPEP-HLVWRRENDLALPK--------GSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTF-LVKPKDQRVGMNGIAKFECVVD-GNPPPSIFWTKEGN---QELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPII---AMGPANQTLPVNT--IAVLPCQASGNPKP-SINWFKNQQPLYAVES--SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
+ +GD L C RG+ ++W G V + EP T T T L+I + G Y C+A N G L+VN P + S K G L C V+ EP + W + D+ + G++V +L+I G Y+C ATN G + S L V+ P V +A+ C V G+ P I W+ +G+ E + T G ++ L+I V G Y C A + AGS A L V + PPII + G PVN +A L C +P SI W + + S + + + L+I ++ + YTC A ++ G ++SA L + +P
Sbjct: 1039 VKEGDITQLTCLVSRGDEPVRITWSLKGDV---ISSEPSITTTMFGTRTSLLMITNVGYRHSGXYTCRASNAAGXATHS-AELNVNESPSILAFSFGSDSVKEGDMTQLTCFVSRGDEPLTITWSLKGDVVSSEPSITTTMIGTRVS--MLMITNVGYRHSGTYTCRATNSGGTATHSAELKVNEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVSSEPSITTTMIG----TRTSMLMITNVGYRHSGSYTCRASNAAGSATHSASLLV-----KEPPIINPFSFGAE----PVNEGDVAQLTCLVRRGDEPLSITWSLKGDIISSEPSMTTTMFGTKASMLMITDVGYRHSGTYTCRASNDGGFDTYSAELKINEP 1410 HSP 8 Score: 80.4925 bits (197), Expect = 1.799e-15 Identity = 97/406 (23.89%), Postives = 158/406 (38.92%), Query Frame = 0
Query: 5 FSLGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTS-KGGSEVTLICLVAGDPEP-HLVWRRENDLALPKGSQVDKGI------LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHP-----TFLVKPKDQRVGMNGIAKFECVV-DGNPPPSIFWTKEGN---QELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP-SINWFKNQQPLYAVESSRIMIEASGT--LVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNII 390
FS G D + +GD L C RG+ +++W G V+ + T L+I + G Y C+A N GT L VN PI + S G L C V+ EP + W + D+ + S I L+I G Y+C A+N G + S L V P +F +P V +A+ C+V G+ P SI W+ +G+ E + T G ++ L+I V G Y C A + G A L++ ++PP I + + + A + C + +P +I W + + + S + + T L+I + + +TC A + +G +S S+ +P P I+
Sbjct: 1128 FSFGSD--------SVKEGDMTQLTCFVSRGDEPLTITWSLKGDVVSSEPSITTTMIGTRVSMLMITNVGYRHSGTYTCRATNSGGTATHS-AELKVNEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVSSEPSITTTMIGTRTSMLMITNVGYRHSGSYTCRASNAAGSATHSASLLVKEPPIINPFSFGAEP----VNEGDVAQLTCLVRRGDEPLSITWSLKGDIISSEPSMTTTMFG----TKASMLMITDVGYRHSGTYTCRASNDGGFDTYSAELKI----NEPPQIAPFSFSPHVIDEGSYAQISCSVTKGDEPLTITWSLHGAVISSEPSITTTMIGTRTSILIISEVGYRHSGKFTCRASNPAGSVRYSTEXSINEP--PRIV 1510 HSP 9 Score: 78.9518 bits (193), Expect = 5.090e-15 Identity = 97/396 (24.49%), Postives = 173/396 (43.69%), Query Frame = 0
Query: 10 DFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTE--------TGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALP---KGSQVDKGILVIPKAE-PHDEGMYSCEATNPVGKVSQS-VVLTVHAHPT---FLVKPKDQRVGMNGIAKFECVVD-GNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP-SINWFKNQQPLYA---VESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
+F ++T + G + C + +G+P P++ W GK E+++E +T+ T L I G Y CQA + VG + S L+V P ++++ + G + + C VAG P ++VW ++ LP + + G L+I + D +Y+C A N G ++S + ++V P F G+ A+ C+V G+ P I W+ +G+ G T Q+ + L I V G Y C+A + AG L V ++PP ++ + L A L C S +P +++W + + + +++I S L I+++ + +YTCT+ +E+G + SA L V +P
Sbjct: 273 EFISHFEETIVKPGGYLSISCIA-KGDPSPNIKWFIYGK---EVKNE-GAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVG-KISHMAKLNVFGAP-YVKKMRPMKVVAGKSMFITCPVAGYPISNIVWEKDGR-QLPFNDRQTVFPNGTLIISDIQRKEDASVYTCVAXNDEGYSARSDLEVSVMERPEIVPFFFGSDSVDEGL--YAQVSCIVQRGDTPIKISWSLKGDTVSSEPGMTTSQVG-PRASILSIHSVGYRHSGTYTCTAQNKAGIISYSTSLTV----NEPPSLMPLTFGRDILDEGEFAQLICIVSKGDEPLTMSWTLKGDDVSSEPGLSTTKIGTRTS-ILTINSVGYRHSGVYTCTSQNEAGVATQSAELKVNEP 652 HSP 10 Score: 75.0998 bits (183), Expect = 8.718e-14 Identity = 103/424 (24.29%), Postives = 169/424 (39.86%), Query Frame = 0
Query: 20 IAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPI---FIQRPEDVTSKGGSEVTLICLVAGDPEP-HLVWRRENDLALPKGSQ------VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPT---FLVKPKDQRVGMNGIAKFEC-VVDGNPPPSIFWTKEG---NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQAS-GNPKPSINWFKNQQPLYAVESSRIMIEASGT--LVIDNLRMEDTALYTCTAGSESGETSWSASLSV-EDPKNPNIIFHKT-PDPSTFPQPPTKPKIVNRRSTSVTISW 421
+ +GD A L C +G+ ++W G V+ + T T L+I + G Y C+A N G+ +L V PI F E V G L CLV EP + W + D+ + S +L+I G Y+C A+N G + S L ++ P F P + A+ C V G+ P +I W+ G + E + T G ++ L+I V G + C A + AGS + ++PP I+ ++++ + A L C S G+ SI+W + + S + T L+I ++ + YTC A + +G +++++L V E P+ F K + F Q +VNR +TI+W
Sbjct: 1231 VNEGDMAQLACFVSKGDEPLRITWSLKGDVVSSEPSITTTMIGTRTSMLMITNVGYRHSGSYTCRASNAAGSATHS-ASLLVKEPPIINPFSFGAEPVNE--GDVAQLTCLVRRGDEPLSITWSLKGDIISSEPSMTTTMFGTKASMLMITDVGYRHSGTYTCRASNDGGFDTYSAELKINEPPQIAPFSFSP--HVIDEGSYAQISCSVTKGDEPLTITWSLHGAVISSEPSITTTMIG----TRTSILIISEVGYRHSGKFTCRASNPAGSVRYSTEXSI----NEPPRIVPFSFGSESIDEGSFAQLTCSISHGDVPVSISWSLKGDVISSEPSITTTMVGGRTSILIISSVGYRHSGDYTCNAKNIAGMVTYTSTLLVNEAPEIGPFSFSKNIMNEGDFAQLTC---VVNRGDLPLTITW 1638 HSP 11 Score: 71.2478 bits (173), Expect = 1.148e-12 Identity = 105/435 (24.14%), Postives = 171/435 (39.31%), Query Frame = 0
Query: 11 FREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTL---TETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPI---FIQRPEDVTSKGGSEVTLICLVAGDPEP-HLVWRRENDLALPKGS------QVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFL-VKPKDQRVGMNGIAKFECVVD-GNPPPSIFWTKEGN---QELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP-SINWF---KNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPK--NPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISW 421
F EP + +GD A L C RG+ S++W G ++ EP T T+ L+I D G Y C+A N G ++ L +N P F P + GS + C V EP + W + + S IL+I + G ++C A+NP G V S +++ P + + + A+ C + G+ P SI W+ +G+ E + T G + L+I V G Y C+A ++AG + L V ++ P I + + A L C + P +I W +N + +S + S LVI++ + YTC A +++G + SA V +P P ++ + F Q +VNR +TI+W
Sbjct: 1322 FGAEP----VNEGDVAQLTCLVRRGDEPLSITWSLKGDII---SSEPSMTTTMFGTKASMLMITDVGYRHSGTYTCRASND-GGFDTYSAELKINEPPQIAPFSFSPHVIDE--GSYAQISCSVTKGDEPLTITWSLHGAVISSEPSITTTMIGTRTSILIISEVGYRHSGKFTCRASNPAGSVRYSTEXSINEPPRIVPFSFGSESIDEGSFAQLTCSISHGDVPVSISWSLKGDVISSEPSITTTMVG----GRTSILIISSVGYRHSGDYTCNAKNIAGMVTYTSTLLV----NEAPEIGPFSFSKNIMNEGDFAQLTCVVNRGDLPLTITWSFHGENIGTNTGIITSNLGPRMS-MLVINSAQHHHQGEYTCQAINKAGIRTHSAEXKVNEPPLIAPFSFANEVLNEGDFAQVTC---VVNRGDLPLTITW 1734 HSP 12 Score: 70.8626 bits (172), Expect = 1.708e-12 Identity = 96/398 (24.12%), Postives = 153/398 (38.44%), Query Frame = 0
Query: 22 QGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIF--IQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPK--------GSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTF-----------------LVKP---KDQRVGMNGIAKFECVV-DGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQA-SGNPKPSINWFKNQQPL---YAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
+GD A L C RG+ +++W +G+ + LVI A QG+Y CQA N G R VN P+ +V ++G + GD + W + P G ++ +LVI A+ H +G Y C+A N G + S L V+ TF L+ P ++ + A+ CV+ G+ P I W+ GN +G + + L I V G Y C A + AG A L+V ++PP I + + A L C SG+ S+ W + + + ++ + S LVI+++ YTC A + +G S++A L V +P
Sbjct: 1617 EGDFAQLTCVVNRGDLPLTITWSFHGENIGTNTGIITSNLGPRMSMLVINSAQHHHQGEYTCQAINKAGIRTHS-AEXKVNEPPLIAPFSFANEVLNEGDFAQVTCVVNRGDLPLTITWSFHGENIGPNTGISTNNLGPRIS--LLVINSAQHHHQGEYICQAQNKAGIQTHSAELKVNG--TFILMRVADYFYFWFSEPPLIAPFSFANEVMNEGDFAQISCVITSGDLPLLITWSFHGNTIGTNTGIATTNLG-PRMSVLAINSVNYSHQGKYTCQAKNKAGIRTHTAELKV----NEPPMIGPFAFPKEVMNEGNFAQLTCVVTSGDLPLSVTWTFHGDKVGTQTGITTTNLGPRLS-LLVINSVGYRHRGNYTCXASNNAGHDSYTAKLRVNEP 2003 HSP 13 Score: 68.9366 bits (167), Expect = 6.884e-12 Identity = 102/405 (25.19%), Postives = 160/405 (39.51%), Query Frame = 0
Query: 5 FSLGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVK-ADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRP--EDVTSKGGSEVTLICLVAGDPEPHLV-WRRENDLALPKGSQ------VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFL-VKPKDQRVGMNGIAKFECVVD-GNPPPSIFWTKEG---NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP-SINWFKNQQPLY---AVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNII 390
+ GDD E G+ A L C G+ +++W G V+ +E G + AV G Y C N G+ +S L VN P + ++V ++G ++C+V EP + W + S +L I G+Y+C+ATN G SQ VL V+ P L + + A+ C+V G+ P SI W+ G + E S G S+ L IQ V G Y C A + AGS A L I ++ P I+ +++ I L C + +P +I W + ++ ++ I AS L+I N+ + YTC A +G + SA L V + +P+I+
Sbjct: 658 LTFGDDVLNE--------GEFAQLFCIVRVGDEPLTLTWALQGDVIS-----------SEPGLSTTQLAVGYXHSGTYTCTVTNKAGS-DSQSAILKVNEPPRILPLTFGQEVMNEG-QFAQVVCIVTEGDEPLSIGWXLQGXXIXSXPSLSXSSLGTRTSMLTIQSIGYRHSGVYTCKATNKAGSGSQXAVLRVNEPPEILPLSFGKPSMDEGEFAQIVCIVSRGDEPLSITWSLYGAVISSEPSISTNLLG----SRXSMLXIQSVGYRHNGIYTCQAKNEAGSMRQSASL----IVNESPSILPFSFGAESVKEGDINQLTCLVNRGDEPLTITWSLKGDVVSSXPSITTTMIGTRAS-MLMITNVGYRHSGTYTCRATXSAGXATHSAELKVNE--SPSIL 1030 HSP 14 Score: 64.6994 bits (156), Expect = 1.381e-10 Identity = 54/214 (25.23%), Postives = 88/214 (41.12%), Query Frame = 0
Query: 14 EPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKV------------LHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN--------DLALPKGSQVDKGIL---VIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRV 204
EP D AQG A + C+S G P+P + W K + +L+ R G+L+I++ K+++G Y+C+A N +G S + V P F + T+ L C G+ ++W N D + +D G+L I + E D +++C ATN G S+ L + +P P D +V
Sbjct: 2108 EPTDKHFAQGSEAKIDCKS-DGFPQPKIVWRKAIGLPVSNEQXPTPSDYKDLDVSNDRNMKIIDGTLIIKNIQKSNEGFYLCKASNGIGGI-SAVAKISVQAPPSFEIKKRTQTALISDNTVLQCEAKGEKPIGVLWNMNNKRLDAKVDDRYTIREEVIDDGVLSTISIKRTERGDSALFTCVATNAFGSDDTSINLIIQENPE---TPYDLKV 2316 HSP 15 Score: 55.0694 bits (131), Expect = 1.098e-7 Identity = 54/221 (24.43%), Postives = 94/221 (42.53%), Query Frame = 0
Query: 264 FYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETG-WVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGM 483
F+V + S+ G +K + +T + P I + G + + I LPC A G P P+++W + P+ E+ RI G+L I + +D A YTC ++ G+ + L V P P PP + + + S+T+ + +S + G+T+ Y + E G W A++ SE + ++ L Y A N G+
Sbjct: 2688 FWVTAHTSIGGGPRSKK-VTLTPTDRIPAKIASFGNSYSAIAKTDIQ-LPCIAVGFPIPALHWKIDGMPI--PENDRIRQLPDGSLQITRISKDDAAQYTCMVNNKYGQDTVIHQLIVNGP----------------PDPP-EMSLTAQTXDSITVKIKPTKNVDKSSIHGFTIHY---KPEFGDWSTASVPYGSEEFILDELLCGQRYHLYATAYNDIGI 2884
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000005601 (supercontig:LSalAtl2s:LSalAtl2s2:790586:820681:1 gene:EMLSAG00000005601 transcript:EMLSAT00000005601 description:"snap_masked-LSalAtl2s2-processed-gene-8.21") HSP 1 Score: 170.629 bits (431), Expect = 2.344e-43 Identity = 177/670 (26.42%), Postives = 279/670 (41.64%), Query Frame = 0
Query: 79 GDYVCQARNMVGTRNSGP-----GTLHVNVKPIFIQRPE-DVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKGI------LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGI-AKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKP--SINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV-EDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSF-------EMETGWVVAALRITSETYTV--NNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWK---VLISSKYVEGFFVRFRDMSGGSQKFNMKTVMK--QDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHV-----KGNGSTFHSNVTLNATTMH------FILSNLSLNEQYTIRASAFTQAGLGPFSRPVT 707
G Y CQ R G GP T+ V KP F +R + G+++++ C V G PEP ++W R N + + + + L I D GM+ C A N G V+ L V LV P ++G C +G P P++ W Q T G++ + +DG+++I VR D G Y C + AG+T +A+L V + Q I P + + + +A L C+ S + ++W+ + + A S RI I GTL I R D YTC S G A+L V E P P I+ + ++ N + SV +SW G SP+I + ++Y S + + W+ A I+S T+ +NLK ++Y+F + A NS G P S S RV L S L L A S T I + W+ + + G+ +R+R ++ + V K Q N Y+IT L + +YE+ + F G S+S+ ++T P + P+ V A+ ++ + ++ PP PQ NGI QGY++ + S VT+ ++ ++ NL Y + +T G G S P T
Sbjct: 298 GVYSCQVRMRSG---GGPTLTKDATVKVTEKPYFYRRMSLETFGDFGNDISIPCHVKGIPEPSVIWYR-NGIPIDEVPNTRYSVDPEDRSLKINFMRNEDSGMFQCSAVNYAGDVNGYTWLRVKTSSPVLVDPPKNTSALDGKDVSIPCKAEGAPLPNVTWYFNDGQI-----TYSGRIQILEDGSMLISKVRSTDQGKYSCIRSNTAGNTRGEAWLGVL-VRXQ----ITSPPVDSKVILGHVASLHCKVSSDQDVPYEVHWYHEGRMINAAMSHRINILDDGTLKIAEARASDAGDYTCEVKSTGGNDHRVAALDVIELPYAPTRIYAE--------------RVANAQK-SVNVSWTPGFD-GNSPIIKFIIQYHSVPQSGPIPKDDLNWITALANISSTARTILLSNLKSSAAYIFRLSAVNSVGEGPQSLPSNRV-VLPQEPPSGPPLGLVG---------------SARSETEIMIQWQPPNEQAQNGDILGYIIRYRLFGYNDSPWSYRNVSKPLQRN---YLITELITWKDYEIQIAAFNNKGTGAYSSSIKLKTREGVPYSAPSGVRAQAIDSSVVRVWWLPPDPQKINGINQGYKLQAWIGDPRDTDIKPQSTVTVAPDLLNPLSEQTAVIDNLKPWTAYNVTVLCYTSPGDGKRSPPET 918 HSP 2 Score: 100.138 bits (248), Expect = 1.520e-21 Identity = 108/399 (27.07%), Postives = 184/399 (46.12%), Query Frame = 0
Query: 20 IAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPE-DVTSKGG-SEVTLICLVAGDPEPHLVWRRENDLAL--PKGSQVDKGILVIPKAEPHDEGMYSCEATNP-VGKVSQSV--VLTVHAH------PTFLVKPKDQRVGMNG-IAKFECVVDGNPPPSI--FWTKEG----NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSA--LSVAGSTLAK-AYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASG-TLVIDNLRMEDTALYTCTAGSESGET---SW-----SASLSVEDPKN 386
+A+ T +L+CQ+ G+P+P W ++G VL E E T + I+ +KAD G+Y C ARN GT S + V F P D+ K G + + ++A PEP ++W+ ++ L K + + LVI + D Y ATN +G+ S L V+ P ++ PKD V + +++ +C+ + P I W K+G ++ +S + + TL + V + G Y C S G TL K A ++VT ++P M N I++ PC G P+PS+ W++N P+ V ++R ++ +L I+ +R ED+ ++ C+A + +G+ +W S+ + V+ PKN
Sbjct: 51 VAERRTKILQCQA-VGSPQPQYRWTRDGVVLSEFSSE----TFYK-----IQSVMKADAGNYQCAARNTAGTILSDTIPITVAYMTPFDPAPNPDLQVKAGHAAIFKFPVLASVPEPSVIWQTDDGTQLYGVKYAMTSENDLVILSVDSSDSRRYRTRATNTQLGQEENSPYGFLEVYGEDGADVPPEIVIPPKDLTVNKDTPVSELQCIANARPLSEIELTWHKDGVPIDETQVSYS------FNDLWNRTLSLLRVNLDHSGVYSCQVRMRSGGGPTLTKDATVKVT---EKPYFYRRMSLETFGDFGNDISI-PCHVKGIPEPSVIWYRNGIPIDEVPNTRYSVDPEDRSLKINFMRNEDSGMFQCSAVNYAGDVNGYTWLRVKTSSPVLVDPPKN 429 HSP 3 Score: 96.2857 bits (238), Expect = 2.697e-20 Identity = 99/390 (25.38%), Postives = 161/390 (41.28%), Query Frame = 0
Query: 17 DTRIAQGDTAVLKCQSPRGNPKPSVSW-LKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARN-MVGTRNSGP-GTLHV------NVKPIFIQRPEDVTSKGGSEVT-LICLVAGDP--EPHLVWRRENDLALP-KGSQVDKGI-------LVIPKAEPHDEGMYSCEATNPVG---KVSQSVVLTVHAHPTFLVKPKDQRVGMNG-IAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVS-QDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
D ++ G A+ K P+PSV W +G L+ + + +T LVI +D Y +A N +G + P G L V +V P + P+D+T + V+ L C+ P E L W ++ +P +QV L + + G+YSC+ G +++ + V P F + + G G C V G P PS+ W + G + + + V +D +L I +R ED G + CSA++ AG +L V P++ P N + +PC+A G P P++ W+ N + S RI I G+++I +R D Y+C + +G T A L V
Sbjct: 135 DLQVKAGHAAIFKFPVLASVPEPSVIWQTDDGTQLYGV-----KYAMTSENDLVILSVDSSDSRRYRTRATNTQLGQEENSPYGFLEVYGEDGADVPPEIVIPPKDLTVNKDTPVSELQCIANARPLSEIELTWHKD---GVPIDETQVSYSFNDLWNRTLSLLRVNLDHSGVYSCQVRMRSGGGPTLTKDATVKVTEKPYFYRRMSLETFGDFGNDISIPCHVKGIPEPSVIWYRNG---IPIDEVPNTRYSVDPEDRSLKINFMRNEDSGMFQCSAVNYAGDVNGYTWLRVKTSS----PVLVDPPKNTSALDGKDVSIPCKAEGAPLPNVTWYFNDGQI--TYSGRIQILEDGSMLISKVRSTDQGKYSCIRSNTAGNTRGEAWLGV 507 HSP 4 Score: 75.485 bits (184), Expect = 5.502e-14 Identity = 76/311 (24.44%), Postives = 129/311 (41.48%), Query Frame = 0
Query: 400 FPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGW--VVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYV---EGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRP 705
P PP + + N + SV + + G S + +TV+ S W + V NL P Y + A N G S PS+ + + +T++A H V +I ++ +T +RV W L ++ G+ + +R ++ S + N ++ + +++V+ L +FT YEV L + PS + +T P A P V AE + T+ + +H NGIL+GY+V+ G F + T L+ L +Y I+ A+T+ G G S P
Sbjct: 1023 LPSPPIRLAVSNIQPFSVYLQFTPGFD-GNSSITKWTVQALS-ARNASWTSIFDEYDPKGNAILVKNLIPYMEYSLRLIANNVVGASEPSEPTRQFQTIQA------------PPKHAPQNV----TIRSMXATELRVRWIPLSQGEWYGIPRGYNISYR-INKDSSELNSISI-EDPTRNTFVLDGLEEFTLYEVVLHAYNDLGTSDPSTVILARTREAVPGAGPNDVSAEATSSTTILVRWGDVLKRHSNGILEGYKVYFGAKGVPFQYKNIGSNQTHQTTLTELKKYTEYAIQVLAYTRIGDGALSAP 1313
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000003860 (supercontig:LSalAtl2s:LSalAtl2s209:184550:379550:-1 gene:EMLSAG00000003860 transcript:EMLSAT00000003860 description:"maker-LSalAtl2s209-augustus-gene-4.12") HSP 1 Score: 168.318 bits (425), Expect = 1.328e-42 Identity = 182/734 (24.80%), Postives = 295/734 (40.19%), Query Frame = 0
Query: 10 DFREEPDDTRIAQGDTAVLKCQSPRGNPKPSV--SWLKNGKVLHELEDEPKRVTLTETGSL---VIRDAVK--ADQGDYVCQAR-NMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVA--GDPEPHL----VWRRENDLALPKGSQVD---KGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQ--DGTLVIQGVRKE-DGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV--------------------EDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLE-EVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDM-SGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGP 701
+F +EP D + + ++ +L C + + V W +NG L + D R L GSL I +++ D+G Y C A +G S L V P F P D+ G CL+ + E L + R ++D L ++ G+L I EP D YSC + S V++ P F+VKP+++ V +C +G P P I W K+G M +H +++ G+L IQ + E D G Y C A + S A +EV + P + P+NQ C+ P + W+KN + + V S I L I L D +Y C A + G SA L V + + IF P P + + +T++W + + ++GYTV Y E V+ + R + L P +SY F V A N +G S+M + DD+++ V+N + +S +I V+W + GF +++ G F T++K+ Y +T+LR++T+Y +++ N EG S + T +D P+ PPT V E + S + PPP + +NGI+ GY++ K G T +A+ F++ L + +Y ++ +A T G GP
Sbjct: 13 EFTKEPSDIFMKENESGLLLCSASTTSTASPVEYKWKRNGHYL-QYPDFNGRRRLLSNGSLHFSWIYNSLTDPRDEGIYQCVAXVKGLGKIVSKTAVLIVKDPPSFELEPSDLQVFLGQTAAFQCLLETPDNSEDELSSDNIQRLKDDQPLILNHRISIKPSGMLEIINVEPSDRAKYSCVYGEELKTSS-----LVNSPPNFIVKPRNRAVLQGTTLILDCAANGVPQPEITWLKDGASLDM----SHLDSRLTKLGKGSLKIQDLNAETDSGAYQCRAENTEDSQDVTAIVEVHSA-----PEFVVRPSNQMCSAKGDITFECKVQSFPPAKVQWYKNGELI--VTSDYFQIVDGSNLRILGLLDSDAGMYQCMATNSVGNVQSSAQLKVISNVVSPSLSSVSSSLDSSPSNDDQGSFIFLDXP------SAPIXFHVAIXATRFITLTWS-SPLNPEGTILGYTVYYKEKGSERERVMNSTRGELSEVNIQGLTPGTSYEFRVLAFNENGRGEYSEM--------ITVSTQDDIDVPGPVRN---------LRCRPTASFSILVAWDTPSTDT---GFITQYKVCHRQGEYGFERSTIVKKMK---YHLTDLREYTDYTFWVSASNANGEGVHSEEIKCMTYSDIPADPPTNVTLEAASSRSILLRWEPPPKESQNGIISGYKLRYKKKGRGKSEIFTTDASRRLFVIPKLFVGTEYQVKIAAMTVNGTGP 699 HSP 2 Score: 55.0694 bits (131), Expect = 1.105e-7 Identity = 69/260 (26.54%), Postives = 115/260 (44.23%), Query Frame = 0
Query: 460 YTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSW------KVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHV-QTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGST----FHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTF 708
+ + +LKP+S YV +RA N+ G P + E VRT + EEV LL V L +I +SS +I ++W K + S Y + VR+ ++ K+ + + + I +LR EYE F K P + + + +TL PS+ P ++ I ++ + LS P++ NG + GY + N + + A T L+ L ++ +Y + SA G GP S+ V+F
Sbjct: 774 FRITDLKPNSEYVISLRAYNNIGDGRP--IYETVRTQEEYE--------EEVLTPLLPP-VGLHTI-VLSSYSIVLTWVDSSLPKNQVVSDY-RYYIVRYAPVNKSPIKYQYRNSTDLNCM----IDDLRANAEYE-FTVKIIKGRRMSPWSLVAMNKTLEAEPSSAPRDLI--IDSVGDGTVELSWIAPKYPNGRINGYIIQYTKNRKAAEIEWXXEAXVGAXTXA-TLNGLDMDAKYFFKMSARNNKGYGPTSQVVSF 1012
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000004009 (supercontig:LSalAtl2s:LSalAtl2s214:573899:577288:-1 gene:EMLSAG00000004009 transcript:EMLSAT00000004009 description:"maker-LSalAtl2s214-augustus-gene-5.7") HSP 1 Score: 164.081 bits (414), Expect = 1.460e-41 Identity = 141/496 (28.43%), Postives = 227/496 (45.77%), Query Frame = 0
Query: 10 DFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIR---DAVKADQGDYVCQARNMVGTRNSGPGTLHVN-VKPIFIQRPEDVTSKGGSEVTLICLV-AGDPEPHLVWRREND---LALPKGSQVD-KGILVIPKAEPHDEGMYSCEATNPVGK-VSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKE-GNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGP-ANQTLPVNTIAV-LPCQASGNPKPSINWFKNQQP------LYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP--------KNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLI-GYTVEYFSFEMETGWVVAALRI-TSETYTVNNLKPDSSYVFLVR 476
+ P +T + +G+ L+C+S G P P + W KNG L P R+ L + L +R + D GDY C A N G+ +S TL + +K F P D + G ++ L C AG PEP + W+R+ D L K ++D +G L+I + DEG Y C A N K +S++V L V+A P F+ KPKD A +C V G+P P + WTKE G+ ++ ++ + L I+ V D G Y+C+A + AG + ++L ++ PP+I P N + V+ + L C SG+P P + W + L + I+++ GTL I+N + + YTCTA +++G + + V DP N + + +T + I STS+ + W H T + I G+ + Y E V LR + ++ ++NL+ S Y ++
Sbjct: 23 EITHHPLNTTVIRGEPLTLECRS-SGFPPPQIRWFKNGSPLFT---SPDRMILPDGSLLFLRVFHSKKETDSGDYHCTATNSQGSASSHIATLEIAYMKDEFKALPRDTEATLGRDIVLECSPPAGHPEPVVKWKRDGDHLDLTSSKRIKIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKAVRLNVNAPPHFISKPKDINAIAGEEAFIDCRVGGDPYPDVTWTKERGDIDI-------SKLKIVSGKGLRIKNVNSHDSGRYICTAKNRAGXISSSSFLTISI-----PPVITERPLMNVQVRVSEDPIELHCGVSGSPSPFVYWSREADAEAGLLILPGEDKHGIIVKVDGTLYIENPQTMHSGHYTCTAVNDAGSAIARSHVLVYDPIHQDSFPDTNAGLELDEARIVTTLEEGVRISDISAVSSTSLKVKW---HITARHKYIDGFYIRYRKVEDPIVATVRVLRSDLTTSFVIDNLEEHSEYEVFIQ 499 HSP 2 Score: 164.081 bits (414), Expect = 1.739e-41 Identity = 95/285 (33.33%), Postives = 145/285 (50.88%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN-DLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKP---KDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQE---LMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEV 284
+ D+F+ P DT G VL+C P G+P+P V W ++G H KR+ + G+L+I+ K+D+G Y C A N+ + S L+VN P FI +P+D+ + G E + C V GDP P + W +E D+ + K V L I HD G Y C A N G +S S LT+ P +P RV + I + C V G+P P ++W++E + E L+ G + V DGTL I+ + G Y C+A++ AGS +A++++ V
Sbjct: 116 MKDEFKALPRDTEATLGRDIVLECSPPAGHPEPVVKWKRDGD--HLDLTSSKRIKIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKAVRLNVNAPPHFISKPKDINAIAGEEAFIDCRVGGDPYPDVTWTKERGDIDISKLKIVSGKGLRIKNVNSHDSGRYICTAKNRAGXISSSSFLTISIPPVITERPLMNVQVRVSEDPI-ELHCGVSGSPSPFVYWSREADAEAGLLILPGEDKHGIIVKVDGTLYIENPQTMHSGHYTCTAVNDAGSAIARSHVLV 397 HSP 3 Score: 139.813 bits (351), Expect = 6.295e-34 Identity = 80/268 (29.85%), Postives = 147/268 (54.85%), Query Frame = 0
Query: 498 AFAGSDDDLNLEEVQ-NHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFT---MDPAYIKSPVVSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVA-IVMYRRQW 760
+F ++ L L+E + L++ V + I A+SST+++V W + KY++GF++R+R + V++ D S+VI NL + +EYEVF+ P+F+NI G+P++ + T D PS P A +LN T+ ++ + PQH NG L+GY++ +KGNG++ ++ V + I++ ++ N+ Y I+ S G GPFS P+ + D A+ + H + W + ++ V+ +LIF++ + Y+R++
Sbjct: 405 SFPDTNAGLELDEARIVTTLEEGVRISDISAVSSTSLKVKWHITARHKYIDGFYIRYRKVE--DPIVATVRVLRSDLTTSFVIDNLEEHSEYEVFIQPYFQNIVGKPASIKRIITHPDLPSEAPLIREARLLNSTTVFLSWNTISPQHINGPLRGYEILIKGNGTSVNNTVDAETNEVTLIVNGMAPNKIYMIQISVINSIGHGPFSSPLEISKGFFDEAHNPHLPIDHENKD---VKTTWIIAIVA--VLTFVLIFISGVFFYKRKF 665
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000001666 (supercontig:LSalAtl2s:LSalAtl2s128:488193:500323:-1 gene:EMLSAG00000001666 transcript:EMLSAT00000001666 description:"maker-LSalAtl2s128-augustus-gene-5.5") HSP 1 Score: 158.303 bits (399), Expect = 1.393e-39 Identity = 117/398 (29.40%), Postives = 185/398 (46.48%), Query Frame = 0
Query: 9 DDFREEPDDTR-IAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGD---YVCQARNMVGTRNSGPGTLHVNV-------------KPIF--IQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDK--GILVIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAV-LPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVED 383
++F +EP T+ + +G+ + C SP G PKPSV WLK + H+ + R+T+ G+L + K D + Y C A + +H+ V +P F + ++ + G E+ L C+ G P P + WR++ P + +D LVI + D G Y+CEA+N VG S S+ + VHA P F+V+P+ F C DG P PSI W G + + H ++ D + I V K D G Y C+A +V G Y+ V + PP I + P V+ A+ + C+ G PKP + WF N + L R ++ SG L+I +++ D YTC A ++ G+ S S +L+V++
Sbjct: 142 NNFVDEPPKTKTVEEGEPFGIPCNSPTGWPKPSVYWLKMSQNWHKTLNS-SRITVDPEGTLWFSNITKEDSSEDFLYACSASSYFRNEYKVGNKVHLQVIQSGSNAGLQNKREPDFQYANKRSEIALR-GKEMKLWCIFGGTPLPEIRWRKQGGPLPPGRTTLDNYGKTLVIKHVDFEDAGDYTCEASNGVGIAKSYSISIEVHARPRFIVEPEIMITTEGESVDFLCQADGYPTPSIQWIYNGAK--IEKAPPHPNRFITPD-RITITNVSKSDTGNYGCNASNVNGYVYKDVYVNVLDL----PPEITVPPEETAQTVDGKAMTMKCETFGAPKPIVKWFHNNEEL---TGGRYKVQDSGDLLIQDVKFFDAGNYTCKAENKFGKASASGTLTVKE 527 HSP 2 Score: 138.658 bits (348), Expect = 1.863e-33 Identity = 172/689 (24.96%), Postives = 266/689 (38.61%), Query Frame = 0
Query: 35 GNPKPSVSWLKNGKVLHELEDEPKRVTLTETG-SLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKG-----------ILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTV-HAHPTFLVKPKDQRVGMNGIA-KFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQA--SGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRI-TSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQ-PSNSLHVQTLADTPS-APPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGS---TFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGP 701
G P P + W K G L P R TL G +LVI+ D GDY C+A N VG S ++ V+ +P FI PE + + G V +C G P P + W + G++++K + I D G Y C A+N G V + V + V P V P++ ++G A +C G P P + W N+EL T G+ V G L+IQ V+ D G Y C A + G A L V I+ GP + + A C A + I+W KN + + R + + +L I D+ YTC A S SASL V+D NP PS VN S ++W ++P++ Y + Y + W VA + S+T ++ P ++Y F V A N G S PS SE T + D N+E + V+ + I + ++V + ++ D++ + VI N ++ Y++ + + E + + + + + PS AP + +++ SA ++ P NG +GY++ N S F TT F+ S + Y R AF A GP
Sbjct: 280 GTPLPEIRWRKQGGPL-----PPGRTTLDNYGKTLVIKHVDFEDAGDYTCEASNGVGIAKSYSISIEVHARPRFIVEPEIMITTEGESVDFLCQADGYPTPSIQW-------IYNGAKIEKAPPHPNRFITPDRITITNVSKSDTGNYGCNASNVNGYVYKDVYVNVLDLPPEITVPPEETAQTVDGKAMTMKCETFGAPKPIVKWF-HNNEEL-----TGGRYKVQDSGDLLIQDVKFFDAGNYTCKAENKFGKASASGTLTVKEHTR-----ISTGPKDYEVEAGDSATFRCNAVYDSELELDIHWLKNGKLIDFDIEPRFIQSSDQSLTITKTTELDSGQYTCLAKSSLDSAQASASLIVQDVPNP---------PSLL--------WVNCNSEDAVVTWAP-MGDNRAPILNYKMLYNTSFTPDTWEVATDTVPASDTSFKVSMSPWTNYTFKVIARNQVGDSFPSGHSETCLTPERIPFKNPD-NVEGRGTSPNNLVIYWTPMPQIEHNAPKFQYRVYWKKDEEDASWI-IEDIADW-------------RVKELVIDNQPTYSRYQIKVVAHNRIGEAKMKAKPVIGWSGENQPSEAPLNFTLLDVVGPRSAYVSWDPVSLDSINGDFKGYKIQTWTNSSGEEKFREIPWGKDTTEAFVQSFKPFSLNYA-RVLAFNGAYNGP 911 HSP 3 Score: 73.559 bits (179), Expect = 2.018e-13 Identity = 99/384 (25.78%), Postives = 146/384 (38.02%), Query Frame = 0
Query: 27 VLKCQSPRGNPKPSVSWLKNG-----KVLHE-LEDEPKRVTL-------TETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQR-PEDVTSKGGSEVTLICLV-AGDPEPHLVWRR--ENDLALPKGSQVD---KGILV---IPKAEPHDEGMYSCEATNPVG---KVSQSVVLTV-----------HAHPTFLVKPKDQRVGMNGIA-KFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDG-TLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESG 371
+++C++ G P P W KNG +V E + +P R T E G + V +G Y C A N GT S + + F+ P+ T + G + C G P+P + W + +N S++ +G L I K + ++ +Y+C A++ KV V L V P F K + + G K C+ G P P I W K+G G+ + G TLVI+ V ED G Y C A + G +AK+Y +IE P + P CQA G P PSI W N + + I N+ DT Y C A + +G
Sbjct: 55 IIECEA-EGEPAPMYRWEKNGEPYDWQVYDERISQQPGRGTFLITKPRDEEIGEIFDYRRV---EGQYQCFATNEWGTATSNSVYVRKSELNNFVDEPPKTKTVEEGEPFGIPCNSPTGWPKPSVYWLKMSQNWHKTLNSSRITVDPEGTLWFSNITKEDSSEDFLYACSASSYFRNEYKVGNKVHLQVIQSGSNAGLQNKREPDFQYANKRSEIALRGKEMKLWCIFGGTPLPEIRWRKQGGP------LPPGRTTLDNYGKTLVIKHVDFEDAGDYTCEASNGVG--IAKSY--SISIEVHARPRFIVEPEIMITTEGESVDFLCQADGYPTPSIQWIYNGAKIEKAPPHPNRFITPDRITITNVSKSDTGNYGCNASNVNG 424 HSP 4 Score: 56.9954 bits (136), Expect = 2.571e-8 Identity = 62/232 (26.72%), Postives = 95/232 (40.95%), Query Frame = 0
Query: 165 HDEGMYS----CEATNPVGKVSQSVVLTVHAHPT--FLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQD---GTLVIQGVRKED----------GGFYVCSALSVAGSTLAKAYL----EVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQA-SGNPKPSINWFKNQQPLY-AVESSRIMIEASGTLVIDNLRMEDTA---LYTCTAGS 368
H GMYS C + QS + PT L + K ++ + EC +G P P W K G + + + +SQ GT +I R E+ G Y C A + G+ + + E+ D+P P +T+ +PC + +G PKPS+ W K Q + + SSRI ++ GTL N+ ED++ LY C+A S
Sbjct: 3 HKSGMYSFLILCSILPSSIGIVQSPPRMIKQPPTDELLFQVKSRQDENDKPFIIECEAEGEPAPMYRWEKNGEP---YDWQVYDE-RISQQPGRGTFLITKPRDEEIGEIFDYRRVEGQYQCFATNEWGTATSNSVYVRKSELNNFVDEP-------PKTKTVEEGEPFGIPCNSPTGWPKPSVYWLKMSQNWHKTLNSSRITVDPEGTLWFSNITKEDSSEDFLYACSASS 223
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000011601 (supercontig:LSalAtl2s:LSalAtl2s800:5918:93267:1 gene:EMLSAG00000011601 transcript:EMLSAT00000011601 description:"maker-LSalAtl2s800-augustus-gene-1.51") HSP 1 Score: 144.436 bits (363), Expect = 2.935e-35 Identity = 184/715 (25.73%), Postives = 288/715 (40.28%), Query Frame = 0
Query: 19 RIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVK-ADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFI--QRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKGI-----------LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKF-ECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTL-------VIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSF--EMETGWVVAALRITSETYTVN----NLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKY---VEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNA--TTMHFI-LSNLSLNEQYTIRASAFTQAGL 699
+ QG + C G P S+ W ++G R ++ E G+LVIR+A K D+G Y C A N ++G + V P + P + S+G ++ G+ W R+ ++D+GI LVI + G Y+C A+N G S + VL P ++ P+D ++G F C V+G P P++ W K G Y C+A + S ++K + +T PP + + +L C+ +G+ I W +N P+ E +R IE TL VI + +D TC A ++ G LSVED P P +I N S S+ I W H G S ++ Y VEY + E + G ++T+ VN NL+P + Y F V AEN HG+S S + +RT GS + VE++S SST +RV W S + + + + ++++ S M +S ++T+LRKFT Y++ + P+ N EG +S+ P+ PPT L+ +S ++ S P P+ RNGI++GY V + S L T I L++L Y+I AFT+AG+
Sbjct: 11 KAIQGKDLRISCPV-SGYPIQSILWQRDGYK----XPSNGRQSVFENGTLVIREARKLEDEGYYTCSAFNPRDESHTGTIQIQVLSPPTIMPFLFPNKLLSEGMRSAVSCQILEGNLPIRFSWNRDGRPIGEDSPKLDRGIYIRSTDQYSSTLVIEELRFRHRGNYTCVASNSAGYASYTTVLIA---PKWIRIPEDVH-AVDGAEXFIHCEVEGYPKPNLSWNKVTT-------------------------------GMYTCTADNGVRSGISKT-INLTV---NSPPRFLEEKVHLAVRKGEKGILICEVAGDENIDILWRRNGMPISEGEDARYSIENHYTLYGSVSRFVISPILDQDAGPVTCEAVNQYGRDQKDFILSVED----------------IPSSPRGVRISNVGSRSLEIGWEIPHD-GNSHILNYLVEYSNLPDERDKG-EKQRRKMTAPGDAVNLIIQNLRPGTKYKFNVLAENRHGISSESH-AVIIRTQEEPPGSPP-------------RNVEVLSK---SSTELRVLWTSPPISNWNSELLSYKIGYKEVGSNSGLQYMYQTSSSHLSESVLLTSLRKFTSYQIIIWPY--NKEGIGPSSI--------PNGPPTEGRCMSLSPSSLEVLWSKPEPEKRNGIIRGYIVKYYPMEKYYDSEGALQTKETDSEIIRLNSLMKFTNYSINVHAFTRAGV 636 HSP 2 Score: 67.3958 bits (163), Expect = 1.726e-11 Identity = 59/204 (28.92%), Postives = 92/204 (45.10%), Query Frame = 0
Query: 190 HAHPTFLVKP---KDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRK-EDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQA-SGNPKPSINWFKNQQPLYAVESS---------RIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASL 379
H P +VK KD R+ C V G P SI W ++G + ++G+ V ++GTLVI+ RK ED G+Y CSA + + +++ + PP I+ N+ L + + CQ GN +W ++ +P+ E S R + S TLVI+ LR YTC A + +G S++ L
Sbjct: 3 HIRPIPIVKAIQGKDLRIS--------CPVSGYPIQSILWQRDG-----YKXPSNGRQSVFENGTLVIREARKLEDEGYYTCSAFNPRDES-HTGTIQIQVL--SPPTIMPFLFPNKLLSEGMRSAVSCQILEGNLPIRFSWNRDGRPI--GEDSPKLDRGIYIRSTDQYSSTLVIEELRFRHRGNYTCVASNSAGYASYTTVL 188
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000003654 (supercontig:LSalAtl2s:LSalAtl2s199:341388:472828:1 gene:EMLSAG00000003654 transcript:EMLSAT00000003654 description:"maker-LSalAtl2s199-augustus-gene-4.26") HSP 1 Score: 138.658 bits (348), Expect = 1.907e-33 Identity = 162/700 (23.14%), Postives = 270/700 (38.57%), Query Frame = 0
Query: 15 PDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLIC-LVAGDPEPHLVWRRENDLALPKGS-QVDKGI----LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQD---------------------GTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKN-QQPLYAVESSRI-MIEASG------TLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRIT----SETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKF------------------NMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVK 657
P + G +L C G P + W K G+ L R T G+L+I+ +D Y+C +N VG R G L P K ++C + +GD + W ++ + P + Q+ + L+ + + G Y C A+N + + L V P++L+ PKD +C GNP P++ W + + +SG H+S+D G+L++ V ED G Y C + G+ ++ V Q P G N+T A L C A GN ++ W ++ + A +SR ++EA+ TL I ++R D+ + C AG++ G +S S LSV + P+PP+ I S S+ ++W + G SP++ Y V+Y E W + S + L+P + Y V + N G PS++ V TL+ E++ N+ K V + EA +++ + G++VR+ + N+ + + ++ +LR FT+Y+V++ F + S V TL PS PP V E + TS I P HRNGI+ GY++ +
Sbjct: 7 PRNKXAVSGGDVLLLCPV-SGYPIERIVWSKEGRELRG-----PRPTPLSNGTLLIQKVEASDATTYICVVQNRVGQRAQGKIHLLAIKAPKIAPFHFAPDLKEDDRAQVVCSITSGDLPIQISWEKDGKVLEPDSNIQIQNNVFGSSLLFFRLKAQHSGFYKCIASNAAETTNFTSQLVVKVPPSWLLAPKDIVTRHRSQVVLDCQASGNPRPTVSWRRAHSSS--YSGN-----HISRDKDGSYYEYVQTSEPGRSFHPNGSLILHHVTPEDEGQYRCEVQNGVGAISKSVFVTV-----QVPVYFGTGSQNRTAVAGKDASLVCNALGNSPINLEWKTGLREKIGARMTSRYSIVEANSSGGVLSTLTIRSVRRSDSGEFICEAGNDFGTSSISIFLSVVES----------------PEPPSSVHITELSSRSINLAWYPAYD-GNSPVLQYIVQY--KEGGVHWRYHHNKTVGGGESTGIKLGGLRPYTEYNMRVMSVNEVGAGDPSEVITAV-TLQ-----------EDIANYDY-KAVFMYPPEA------------SLANGRILGYYVRYGATDESLDEIRHQQHTNPNNGLISSSSPNLARMTVRGGRTQALVGDLRPFTKYQVYVVAFNEKGLSPHSTKTIVSTLEGVPSGPPLKVRCEAESSTSLSIAWRQPEVAHRNGIILGYKISYR 644 HSP 2 Score: 101.293 bits (251), Expect = 6.430e-22 Identity = 92/312 (29.49%), Postives = 138/312 (44.23%), Query Frame = 0
Query: 11 FREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQ-GDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE-------NDLALPK-GSQVD-------------KGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGI-AKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQD------GTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPP 293
F PD + + D A + C G+ +SW K+GKVL D ++ GS ++ +KA G Y C A N T N L V V P ++ P+D+ ++ S+V L C +G+P P + WRR N ++ K GS + G L++ P DEG Y CE N VG +S+SV +TV P + R + G A C GN P ++ W K G +E + + T V + TL I+ VR+ D G ++C A + G++ +L V +E PP
Sbjct: 96 FHFAPD---LKEDDRAQVVCSITSGDLPIQISWEKDGKVLEP--DSNIQIQNNVFGSSLLFFRLKAQHSGFYKCIASNAAETTNF-TSQLVVKVPPSWLLAPKDIVTRHRSQVVLDCQASGNPRPTVSWRRAHSSSYSGNHISRDKDGSYYEYVQTSEPGRSFHPNGSLILHHVTPEDEGQYRCEVQNGVGAISKSVFVTVQV-PVYFGTGSQNRTAVAGKDASLVCNALGNSPINLEW-KTGLREKIGARMTSRYSIVEANSSGGVLSTLTIRSVRRSDSGEFICEAGNDFGTSSISIFLSV--VESPEPP 397
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000006220 (supercontig:LSalAtl2s:LSalAtl2s33:28635:33298:1 gene:EMLSAG00000006220 transcript:EMLSAT00000006220 description:"maker-LSalAtl2s33-augustus-gene-0.24") HSP 1 Score: 118.627 bits (296), Expect = 2.866e-27 Identity = 92/300 (30.67%), Postives = 130/300 (43.33%), Query Frame = 0
Query: 6 SLGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-----KPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALP--KGSQVDKGI-LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVK---PKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPI 294
SL D P T + +G+T L C + G P P+ SW K+G L D R ++ E G+L I +A D Y C A N +G R S + VN+ KPIF P + S G V C+ G P P W + N + + + D G L I D G Y+C A N G S LTV + PT +V P D G + C G P PSI W K+G ++ +S DG+L++ V + G Y C A + A + + A L V +++ P+
Sbjct: 74 SLSDVGSYHPTRTDVLKGETIRLTCGAT-GFPTPTFSWFKDGGRLSSFHD---RFSVGEDGTLTITNAQLDDSAXYRCSATNYLG-RASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTCISTGHPYPDTTWWKNNRIVSSDNRITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTVQSRPTPIVMLEIPHDMNAGAGSTVQLACRASGYPKPSITWKKDGRP----ITESYLAYRLSPDGSLILFNVSRVYNGNYQCVASNDAETKQSNAVLSVFIDKEKLYPL 364 HSP 2 Score: 112.079 bits (279), Expect = 3.074e-25 Identity = 99/380 (26.05%), Postives = 158/380 (41.58%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGS-LVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALP---KGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVH-----AHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGT-LVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMG-PANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
+EP D + G + C++ G P P + W K+G+++ R +L S L I A +D G Y P G + L C G P P W ++ + S + G L I A+ D Y C ATN +G+ S + + V+ + P F P++ + GI +F C+ G+P P W K N ++ ++ ++ VS G L IQ VR +D G Y C A + G + A L V + +P PI+ + P + + L C+ASG PKPSI W K+ +P+ + + G+L++ N+ Y C A +++ +A LSV
Sbjct: 18 KEPTDADVNPGGSTFFTCKAS-GVPSPFIIWKKDGQIVSR-----SRTSLVNGESTLRISSASLSDVGSY----------------------------HPTRTDVLKGETIRLTCGATGFPTPTFSWFKDGGRLSSFHDRFSVGEDGTLTITNAQLDDSAXYRCSATNYLGRASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTCISTGHPYPDTTWWK--NNRIV---SSDNRITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTV---QSRPTPIVMLEIPHDMNAGAGSTVQLACRASGYPKPSITWKKDGRPITESYLA-YRLSPDGSLILFNVSRVYNGNYQCVASNDAETKQSNAVLSV 354 HSP 3 Score: 99.7525 bits (247), Expect = 1.730e-21 Identity = 73/290 (25.17%), Postives = 121/290 (41.72%), Query Frame = 0
Query: 104 KPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGT-LVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFH 392
+P+ + P D G C +G P P ++W+++ + + + G E+T + S S V + H T ++K + R+ C G P P+ W K+G + F H + V +DGTL I + +D Y CSA + G + A + V PI P N I C ++G+P P W+KN + + + +RI + SG L I N+R++D YTC A + G + SA+L+V+ P ++
Sbjct: 13 RPLITKEPTDADVNPGGSTFFTCKASGVPSPFIIWKKDGQIVSRSRTSLVNG-----------------ESTLRISSASLSDVGSYHPTRTDVLKGETIRL--------TCGATGFPTPTFSWFKDGGRLSSF----HDRFSVGEDGTLTITNAQLDDSAXYRCSATNYLGRASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTCISTGHPYPDTTWWKNNRIVSS--DNRITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTVQSRPTPIVMLE 271
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036496|sp|Q9HCK4.2|ROBO2_HUMAN (RecName: Full=Roundabout homolog 2; Flags: Precursor) HSP 1 Score: 499.59 bits (1285), Expect = 2.837e-156 Identity = 331/942 (35.14%), Postives = 482/942 (51.17%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQ--VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSG---TTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVT-AIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNID-------SYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEIL---NMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRP---VTFTMDPAYIKSPVVSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYED--MSRLHPACSSRDGSMWVNANSGHLHHHWKNGS----DLVVQGQKSGECFYNDNSDGQALYAEVGDTDEVLTTFN--------GFPTSKSDTSNG---DPAPYATTTLAMQNRIRSVHGKTFMALPQHDPSSQDFLSHKTTSSSGGDSL 912
L DDFR+ P D +A G+ A+L+CQ PRG+P+P++ W K+ KV ++D+ +R+++ G L+I + K+D G Y C NMVG R+S P L V +P F++RP + V C V GDP+P + W+++ D LP+G D L I K DEG Y C A N VGK+ S LTV A P F+V+P+DQ V F C GNP P++FW KEG+Q L+F + + VS G L I +++ D G+Y+C AL+VAGS LAKA LEVT + D+PPPII GPANQTL V+ A+L C+A+G+P P I+W K + + R I+ GTL I NLR+ DT YTC A S SGETSWSA L V + K D S P PP+KP++ + SVT+SW+ G P Y +E FS + W A + + YTV L+P++ Y+F+VRA N G+S PS MS+ VRT + + ++ +VQ L D +V L + ++ TT++V+W V ++++G+ V +R SG ++ N+D S V+ NL+K YE+ + P+F +G S S V+T + PSAPP +V + N TS ++ PPPP H+NGI+Q Y++ GN + FH N T++A I+ L QY + +A T AG+G S P + + I S + + D++ +P F+ + V+++ F + +RR+ + L + AV QR + MS SR G + N+G + W S L V SG + G L G D+ T + F T S S+ PYATT + N I +A+ DP + + KT G SL
Sbjct: 131 LRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKD-KV--RIDDKEERISI-RGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKD-DADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLK-EGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTE---SGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPG-TPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRT-QDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSG------LQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRK---KRKGLSNYAVTFQRGDGGLMSN-----GSRPGLL----NAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHE------LAVDLPDPQWKSSIQQKTDLMGFGYSL 1037 HSP 2 Score: 174.866 bits (442), Expect = 2.870e-43 Identity = 122/388 (31.44%), Postives = 181/388 (46.65%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK-RVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVN-VKPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD--KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVE----SSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVED 383
E P D +++G+ L C++ G P P++ W K+G+ + +D+P+ L +GSL V K D+G YVC ARN +G S +L V ++ F Q P DV G L C G PEP + W+++ K ++ G L+I D GMY+C TN VG + S LTV PTFL +P +Q V +F C V G+P P++ W K+ + G+ + D TL I+ D G Y+C A + G A A L V A PP + P +Q + PC+ GNP+P++ W K +Q L+ + +SR + +G L I N++ D Y C A + +G A L V D
Sbjct: 35 EHPSDVIVSKGEPTTLNCKA-EGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDD------ADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRA-----PPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTD 410 HSP 3 Score: 96.2857 bits (238), Expect = 7.285e-19 Identity = 84/297 (28.28%), Postives = 123/297 (41.41%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREND-------------LALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGK-VSQSVVLTVH-AHPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVS-QDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
P ++ P DV G TL C G P P + W ++ + + LP GS I+ +++P DEG Y C A N +G+ VS++ L V F P D V A EC G+P P+I+W K+ + + +S + G L+I RK D G Y C ++ G + E+T E P P NQ + CQ G+P+P++ W K+ L R I+ TL I D Y C A + G+ SA+L+V P
Sbjct: 31 PRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKP-DEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVR------IDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDP-AELTVFER---PTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADL---PRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAP 313
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036483|sp|Q7TPD3.2|ROBO2_MOUSE (RecName: Full=Roundabout homolog 2; Flags: Precursor) HSP 1 Score: 489.96 bits (1260), Expect = 7.089e-152 Identity = 331/946 (34.99%), Postives = 480/946 (50.74%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQ--VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVH----AHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTT---HGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVT-AIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNID-------SYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEIL---NMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTM---DPAYIKSPVVSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYED--MSRLHPACSSRDGSMWVNANSGHLHHHWKNGS----DLVVQGQKSGECFYNDNSDGQALYAEVGDTDEVLTT-----------FNGFPTSKSDTSNGDPAPYATTTLAMQNRIRSVHGKTFMALPQHDPSSQDFLSHKTTSSSGGDSL 912
L DDFR+ P D +A G+ A+L+CQ PRG+P+P++ W K+ KV ++D+ +R+++ G L+I + K+D G Y C NMVG R+S P L V +P F++RP + V C V GDP+P + W+++ D LP+G D L I KA DEG Y C A N VGKV S LTV A P F+V+P+DQ V F C GNP P++FW KEG+Q L+F + + VS G L I +++ D G+Y+C AL+VAGS LAKA LEVT + D+PPPII GP NQTL V+ A+L C+A+G P P I+W K + + R I+ GTL I NLR+ DT YTC A S SGETSWSA L V + K D + P PP+KP++ + SVT+SW+ G P Y +E FS + W A + + YTV L+P++ Y+F+VRA N G+S PS MS+ VRT + + ++ +VQ L D VV L + ++ TT++V+W V ++++G+ V +R SG TV + N+D S V+ NL+K YE+ + P+F +G S S V+T + PSAPP +V + N TS ++ PPP H+NGI+Q Y++ GN + FH N T++A ++ L QY + +A T AG+G S P + + I S + + D++ +P F+ + V+++ F + +RR+ + L + AV QR + MS SR G + N+G ++ W S L V SG + G L G D+ T F T S + PYATT + N I +A+ DP + + KT G SL
Sbjct: 131 LRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKD-KV--RIDDKEERISI-RGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKD-DADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLK-EGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTES---GATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPG-TPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRT-QDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSG----LQASTVWQ--NLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRK---KRKGLSNYAVTFQRGDGGLMSN-----GSRPGLL----NAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHE------LAVDLPDPQWKSSVQQKTDLMGFGYSL 1041 HSP 2 Score: 174.866 bits (442), Expect = 3.742e-43 Identity = 122/388 (31.44%), Postives = 181/388 (46.65%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK-RVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVN-VKPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD--KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVE----SSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVED 383
E P D +++G+ L C++ G P P++ W K+G+ + +D+P+ L +GSL V K D+G YVC ARN +G S +L V ++ F Q P DV G L C G PEP + W+++ K ++ G L+I D GMY+C TN VG + S LTV PTFL +P +Q V +F C V G+P P++ W K+ + G+ + D TL I+ D G YVC A + G A A L V + PP + P +Q + PC+ GNP+P++ W K +Q L+ + +SR + +G L I N++ D Y C A + +G A L V D
Sbjct: 35 EHPSDVIVSKGEPTTLNCKA-EGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDD------ADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTV-RVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTD 414 HSP 3 Score: 94.7449 bits (234), Expect = 1.971e-18 Identity = 83/295 (28.14%), Postives = 122/295 (41.36%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREND-------------LALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGK-VSQSVVLTVH-AHPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVS-QDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVE 382
P ++ P DV G TL C G P P + W ++ + + LP GS I+ +++P DEG Y C A N +G+ VS++ L V F P D V A EC G+P P+I+W K+ + + +S + G L+I RK D G Y C ++ G + E+T E P P NQ + CQ G+P+P++ W K+ L R I+ TL I D Y C A + G+ SA+L+V
Sbjct: 31 PRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKP-DEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVR------IDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDP-AELTVFER---PTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADL---PRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVR 311
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|449061888|sp|G5EBF1.1|SAX3_CAEEL (RecName: Full=Protein sax-3; AltName: Full=Sensory axon guidance 3; Flags: Precursor) HSP 1 Score: 478.404 bits (1230), Expect = 2.854e-149 Identity = 272/714 (38.10%), Postives = 399/714 (55.88%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQV--DKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSG--TTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTA------IEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFA----GSDDDLNLEEVQNHLL-DKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRD--MSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPF---FKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLG 700
L +DFR P + G+ AVL+C PRG P+P VSW K+ K L ++D P R TL G+L+I ++D G Y C A NMVG R S P L V KP F Q P+D+T G+ V C V GDP+P + W+R+N+ + + D L I + +P DEG Y C A NP G + S L V A P+F KP DQ V G A FEC + G P P+ FW+KEG Q+L+F + G+ VS GTL I+ VR+ D G YVC+ ++ AGS+L+KA L+VT +PPP I G NQTL V + A+LPCQASG P P I+W ++ P+ + SRI ++G+L I +L+ DT +YTC A +E GE++WSASL+VED N F + PDPS FP PT+P IVN T V + W +G P+ GY ++Y+S ++ W + S Y + LKP SY+F++RAEN G+ PS S V T + A + +++ + L +++++L ++ I+ST +R+ WK + ++G+++++R + +Q N+ + + ++YV++NL FT YE F+ P+ +I G PSNS+ V T PS PP V +LN+T+ +I+ P NGIL+G+Q+ + G + N+T N L +L Y IR +A + G+G
Sbjct: 131 LREDFRVRPRTVQALGGEMAVLECSPPRGFPEPVVSWRKDDKEL-RIQDMP-RYTLHSDGNLIIDPVDRSDSGTYQCVANNMVGERVSNPARLSVFEKPKFEQEPKDMTVDVGAAVLFDCRVTGDPQPQITWKRKNEPMPVTRAYIAKDNRGLRIERVQPSDEGEYVCYARNPAGTLEASAHLRVQAPPSFQTKPADQSVPAGGTATFECTLVGQPSPAYFWSKEGQQDLLFPSYVSADGRTKVSPTGTLTIEEVRQVDEGAYVCAGMNSAGSSLSKAALKVTTKAVTGNTPAKPPPTIEHGHQNQTLMVGSSAILPCQASGKPTPGISWLRDGLPI-DITDSRISQHSTGSLHIADLKKPDTGVYTCIAKNEDGESTWSASLTVED-HTSNAQFVRMPDPSNFPSSPTQPIIVNVTDTEVELHWNAPSTSGAGPITGYIIQYYSPDLGQTWFNIPDYVASTEYRIKGLKPSHSYMFVIRAENEKGIGTPSVSSALVTTSKPAAQVALSDKNKMDMAIAEKRLTSEQLIKLEEVKTINSTAVRLFWKKRKLEELIDGYYIKWRGPPRTNDNQYVNVTS----PSTENYVVSNLMPFTNYEFFVIPYHSGVHSIHGAPSNSMDVLTAEAPPSLPPEDVRIRMLNLTTLRISWKAPKADGINGILKGFQIVIVGQAPNNNRNITTNERAASVTLFHLVTGMTYKIRVAARSNGGVG 836 HSP 2 Score: 160.999 bits (406), Expect = 7.238e-39 Identity = 121/392 (30.87%), Postives = 181/392 (46.17%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPS---VSWLKNGK-VLHELEDEPKRVTLTETGSLVI------RDAVKADQGDYVCQARNMVGTRNSGPGTLHVN-VKPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRREND----LALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGT-LVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQ-----PLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
E P D +++G A L C + KPS ++W K+G+ V+ E + +TGSL + ++ +D G Y C A N G S G+L + ++ F RP V + GG L C G PEP + WR+++ +P+ + G L+I + D G Y C A N VG +VS L+V P F +PKD V + F+C V G+P P I W K N+ + + + ++++D L I+ V+ D G YVC A + AG+ A A+L V Q PP PA+Q++P A C G P P+ W K Q P Y R + +GTL I+ +R D Y C + +G + A+L V
Sbjct: 35 EHPIDVVVSRGSPATLNCGA-----KPSTAKITWYKDGQPVITNKEQVNSHRIVLDTGSLFLLKVNSGKNGKDSDAGAYYCVASNEHGEVKSNEGSLKLAMLREDFRVRPRTVQALGGEMAVLECSPPRGFPEPVVSWRKDDKELRIQDMPRYTLHSDGNLIIDPVDRSDSGTYQCVANNMVGERVSNPARLSVFEKPKFEQEPKDMTVDVGAAVLFDCRVTGDPQPQITW-KRKNEPMPVT-----RAYIAKDNRGLRIERVQPSDEGEYVCYARNPAGTLEASAHLRV-----QAPPSFQTKPADQSVPAGGTATFECTLVGQPSPAYFWSKEGQQDLLFPSYVSADGRTKVSPTGTLTIEEVRQVDEGAYVCAGMNSAGSSLSKAALKV 410
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036480|sp|O89026.1|ROBO1_MOUSE (RecName: Full=Roundabout homolog 1; Flags: Precursor) HSP 1 Score: 482.641 bits (1241), Expect = 3.440e-148 Identity = 286/772 (37.05%), Postives = 435/772 (56.35%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQ--VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFS---GTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVT-AIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQK------FNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPP--TTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPV-----VSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQ 759
L DDFR+ P D +A G+ AV++CQ PRG+P+P++SW K+G L+D+ +R+T+ G L+I K+D G YVC NMVG R S L V +P F++RP ++ C GDP P + WR++ D LPK D L I K D G Y+C A N VGK S LTV P F+VKP+DQ V + F+C GNP P+IFW +EG+Q L+FS + + VSQ G L I V++ D G+Y+C L+VAGS + KAYLEVT I D+PPP+I GP NQT+ V+ +L C A+G+P P+I W K+ L + + SRI SG L I ++ DT YTCTA + SGE +WSA + V++ P + + DP+ P P+KP++ + +VT+SW+ N +G +P Y +E FS + W AA + +ET+ + LKP++ Y+FLVRA N++G+S PSQ+S+ V+T + + ++ ++VQ L + V+ L + +SS+++ V W V S+Y++G+ + +R SG S F ++T K +S VI +LRK YE+ PFF +G S +TL + PSAPP TV N T+ +T PPP +NG++Q Y+V GN + +H N T++ +T ++ +L +Y++ +A T AG G S P +D +PV VS Q + D++ +P F+ + + +++++F +I +YR +
Sbjct: 129 LRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGS---PLDDKDERITI-RGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKD-DGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGV-LVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVP-VQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATP-TSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKT-QDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRP-SGASHGESEWLVFEVRTPTK----NSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSH--GNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVF-SIWLYRHR 883 HSP 2 Score: 165.622 bits (418), Expect = 2.588e-40 Identity = 119/388 (30.67%), Postives = 186/388 (47.94%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK-RVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQ--VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVV-LTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVE----SSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVED 383
E P D +++G+ A L C++ G P P++ W K G+ + +D+P+ L +GSL V + D+G Y+C ARN +G S +L V + + F Q P DV G + C G PEP + W+++ K + + G L+I D G Y C TN VG+ V LTV P+F+ +P + V ++ A+F+C G+P P++ W K+ + EL S + + D TL I+ V D G Y C A ++ G A A L V Q PP + P +Q + + C+A+GNP+P+I W + +Q L++ + SSR + +G L I N++ D Y C + +G A L V D
Sbjct: 33 EHPSDLIVSKGEPATLNCKA-EGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKD-DGELPKS-----RYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTV-----QEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTD 408 HSP 3 Score: 106.686 bits (265), Expect = 4.937e-22 Identity = 92/302 (30.46%), Postives = 132/302 (43.71%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVW-----RREND--------LALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGK-VSQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEG------NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
P ++ P D+ G TL C G P P + W R E D + LP GS I+ K+ P DEG+Y C A N +G+ VS + L V F P D V + A EC G+P P+I W K+G ++ + G G L+I RK D G YVC ++ G ++ E+T +E P P+N + V+ A C+A G+P P++ W K+ L SR I TL I + D YTC A + G+ SA+L+V++P
Sbjct: 29 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP-DEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRG-----------GKLMITYTRKSDAGKYVCVGTNMVGERESEV-AELTVLER---PSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGEL---PKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP 311
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036500|sp|Q9Y6N7.1|ROBO1_HUMAN (RecName: Full=Roundabout homolog 1; AltName: Full=Deleted in U twenty twenty; AltName: Full=H-Robo-1; Flags: Precursor) HSP 1 Score: 474.552 bits (1220), Expect = 4.828e-145 Identity = 284/772 (36.79%), Postives = 430/772 (55.70%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQ--VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFS---GTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVT-AIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQK------FNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPP--TTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPV-----VSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQ 759
L DDFR+ P D +A G+ AV++CQ PRG+P+P++SW K+G L+D+ +R+T+ G L+I K+D G YVC NMVG R S L V +P F++RP ++ C GDP P + WR++ D LPK D L I K D G Y+C A N VGK S LTV P F+VKP+DQ V + F+C GNP P+IFW +EG+Q L+FS + + VSQ G L I V++ D G+Y+C L+VAGS + KAYLEVT I D+PPP+I GP NQT+ V+ VL C A+G+P P+I W K+ L + + SRI +G L I ++ DT YTC A + SGE +WSA + V++ P + + DP+ P P+KP++ + +VT+SW+ N +G +P Y +E FS + W A + +ET + LKP++ Y+FLVRA N++G+S PSQ+S+ V+T S ++ ++VQ L + V+ L + +SS++I V W V S+Y++G+ + +R SG + F ++T K +S VI +LRK YE+ PFF +G S +TL + PSAPP TV N T+ ++ PPP +NG++Q Y+V GN + +H N T++ +T ++ L +Y++ +A T AG G S P +D +PV VS Q + D++ +P F+ + + +++++F +I +YR +
Sbjct: 168 LRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGS---PLDDKDERITI-RGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKD-DGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGV-LVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVP-VQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATP-TSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTS-QGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRP-SGANHGESDWLVFEVRTPAK----NSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAH--GNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVF-SIWLYRHR 922 HSP 2 Score: 166.777 bits (421), Expect = 1.263e-40 Identity = 120/388 (30.93%), Postives = 186/388 (47.94%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK-RVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD--KGILVIPKAEPHDEGMYSCEATNPVGKVSQSVV-LTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVE----SSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVED 383
E P D +++G+ A L C++ G P P++ W K G+ + +D+P+ L +GSL V + D+G YVC ARN +G S +L V + + F Q P DV G + C G PEP + W+++ K ++ G L+I D G Y C TN VG+ V LTV P+F+ +P + V ++ A+F+C G+P P++ W K+ + EL S + + D TL I+ V D G Y C A ++ G A A L V Q PP + P +Q + + C+A+GNP+P+I W + +Q L++ + SSR + +G L I N++ D Y C + +G A L V D
Sbjct: 72 EHPSDLIVSKGEPATLNCKA-EGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKD-DGELPKS-----RYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTV-----QEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTD 447 HSP 3 Score: 107.457 bits (267), Expect = 2.724e-22 Identity = 92/302 (30.46%), Postives = 132/302 (43.71%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVW-----RREND--------LALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGK-VSQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEG------NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
P ++ P D+ G TL C G P P + W R E D + LP GS I+ K+ P DEG+Y C A N +G+ VS + L V F P D V + A EC G+P P+I W K+G ++ + G G L+I RK D G YVC ++ G ++ E+T +E P P+N + V+ A C+A G+P P++ W K+ L SR I TL I + D YTC A + G+ SA+L+V++P
Sbjct: 68 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP-DEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRG-----------GKLMITYTRKSDAGKYVCVGTNMVGERESEV-AELTVLER---PSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGEL---PKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP 350 HSP 4 Score: 69.3218 bits (168), Expect = 1.188e-10 Identity = 72/257 (28.02%), Postives = 107/257 (41.63%), Query Frame = 0
Query: 193 PTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTL----VIQGVR-KEDGGFYVCSALSVAGSTLA-KAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQ-ASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGE-TSWSASLSV-EDP---KNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTV 437
P + P D V A C +G P P+I W K G + + G+L ++ G + + D G YVC A + G ++ A LEV + D P++ + V AV+ CQ G+P+P+I+W K+ PL + RI I G L+I R D Y C + GE S A L+V E P K P+ + D + F K R T+ WR++ G+ P Y +
Sbjct: 68 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDD----FRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDD-KDERITIRG-GKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEF-------KCEARGDPVPTVRWRKDD--GELPKSRYEI 309
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036462|sp|O55005.1|ROBO1_RAT (RecName: Full=Roundabout homolog 1; Flags: Precursor) HSP 1 Score: 473.781 bits (1218), Expect = 1.109e-144 Identity = 310/939 (33.01%), Postives = 486/939 (51.76%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQ--VDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFS---GTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVT-AIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQK------FNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPP--TTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPV-----VSHPQDVGDLISEPWFLIFLSSIVIVLMLIF-VAIVMYRRQWSGHKSSLGHLA-------VPVQRYED-------------MSRLHPACSSRDGSMWVNANSGHLHHHWK---------NGSDLVVQGQKSGECF--YNDNSDGQ---------ALYAEVGDTDEV--LTTFN--GFPTSKSDTSNGDPAPYATTTLAMQNRIRSVH 881
L DDFR+ P D +A G+ AV++CQ PRG+P+P++SW K+G L+D+ +R+T+ G L+I K+D G YVC NMVG R S + V +P F++RP ++ C GDP P WR++ D LPK D L I K D G Y+C A N VGK S LTV P F+VKP+DQ V + F+C GNP P+IFW +EG+Q L+FS + + VSQ G L + V++ D G+Y+C L+VAGS + KAYLEVT I D+PPP+I GP NQT+ V+ L C A+G+P P+I W K+ L + + SRI SG L I ++ DT YTCTA + SGE +WSA + V++ P + + DP+ P P+KP++ + +VT+ W+ N +G +P Y +E FS + W A + +ET+ + LKP++ Y+FLVRA N++G+S PSQ+S+ V+T + + ++ ++VQ L + V+ L + +SS+++ V W V S+Y++G+ + +R SG S F ++T K +S VI +LRK YE+ PFF +G S +TL + PSAPP TV N T+ +T PPP +NG++Q Y+V GN + +H N T++ +T ++ L +Y++ +A T AG G S P +D +PV VS Q + D++ +P F+ + + +++++F + + +R++ +G S+ + P Y+ ++ PA W N+G+ H+ N + + +C YN+ D + +Y +V ++++ + TFN + +G P PYATT L N I +++
Sbjct: 168 LRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGS---PLDDKDERITI-RGGKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTFGWRKD-DGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRKDGV-LVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVP-VQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLLWQPNLNSGATP-TSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKT-QDVPPTTQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRP-SGASHGESEWLVFEVRTPTK----NSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEERPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSH--GNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLSSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTW--PNTGNSHNDCSINCCTASNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLINNMN 1088 HSP 2 Score: 162.925 bits (411), Expect = 2.134e-39 Identity = 117/388 (30.15%), Postives = 185/388 (47.68%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK-RVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD--KGILVIPKAEPHDEGMYSCEATNPVGKVSQSVV-LTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVE----SSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVED 383
E P D +++G+ A L C++ G P P++ W K G+ + +D+P+ L +GSL V + D+G Y+C ARN +G S +L V + + F Q P DV G + C G PEP + W+++ K ++ G L+I D G Y C TN VG+ V +TV P+F+ +P + V ++ A+F+C G+P P+ W K+ + EL S + + D TL I+ V D G Y C A ++ G A A L V Q PP + P +Q + + C+A+GNP+P+I W + +Q L++ + SSR + +G L + N++ D Y C + +G A L V D
Sbjct: 72 EHPSDLIVSKGEPATLNCKA-EGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTFGWRKD-DGELPKS-----RYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTV-----QEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTD 447 HSP 3 Score: 107.071 bits (266), Expect = 3.637e-22 Identity = 93/302 (30.79%), Postives = 131/302 (43.38%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVW-----RREND--------LALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGK-VSQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEG------NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
P ++ P D+ G TL C G P P + W R E D + LP GS I+ K+ P DEG+Y C A N +G+ VS + L V F P D V + A EC G+P P+I W K+G ++ + G G L+I RK D G YVC ++ G +K +VT +E P P+N + V+ A C+A G+P P+ W K+ L SR I TL I + D YTC A + G+ SA+L+V++P
Sbjct: 68 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP-DEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRG-----------GKLMITYTRKSDAGKYVCVGTNMVGERESKV-ADVTVLER---PSFVKRPSNLAVTVDDSAEFKCEARGDPVPTFGWRKDDGEL---PKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP 350
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036492|sp|Q96MS0.2|ROBO3_HUMAN (RecName: Full=Roundabout homolog 3; AltName: Full=Roundabout-like protein 3; Flags: Precursor) HSP 1 Score: 398.282 bits (1022), Expect = 9.264e-119 Identity = 259/719 (36.02%), Postives = 373/719 (51.88%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKG--ILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTT---HGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVT-AIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQ----PPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEV---QNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLS--PPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTM 710
L DDFR+ P + +A G+ AVL+C PRG+P+PSVSW K+G L E E R+T+ G L++ +K+D G YVC A NM G R S + V +P F++RP + + VT +C V GDP P L WR+E D LP G + L I DEG Y+C A N VG+ S L+VH P + +P+DQ F+C GNPPP+IFW KEG+Q L+F + G+ VS G L I V++ D G+YVC A+SVAGS LAKA LE+ A D PP+I GPANQTL + + LPC+ +GNP+PS+ W K+ Q L + + A+GTL I N++ D Y+C A S +GE +WS L + + + +PDP T P P++P + S+T++W+ N QTG + + Y +E FS W A + ET+TV+ L+P++ Y+FLVRA + G+S PS +SE VRT S +E+ Q L + V L + T++VSW V + V+GF V +R + M ++ + S V+ L T+ ++ + + G S S+ + PS PP V + ++ IT+S PP P +NG++ YQ+ GN S FH N + +L L Y +A T AG+G S PV +
Sbjct: 164 LRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLKEEEG---RITI-RGGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKE-DGELPTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQG-DDLQFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEATWSGWLKMRE------DWGVSPDPPTEPSSPPGAPSQPVVTEITKNSITLTWKPNPQTGAA-VTSYVIEAFSPAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVRT----QDSSPSRPVEDPWRGQQGLAEVAVRLQEPIVLGPRTLQVSWTVDGPVQLVQGFRVSWRVAGPEGGSWTMLD-LQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGAESLSVTRSIPEEAPSGPPQGVAVALGGDGNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSAMLRGLVPGLLYRTLVAAATSAGVGVPSAPVLVQL 864 HSP 2 Score: 149.058 bits (375), Expect = 3.740e-35 Identity = 116/387 (29.97%), Postives = 175/387 (45.22%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPK-RVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICLVA-GDPEPHLVWRREN-DLALPKGS-QVDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES----SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVE 382
E+P D +++G+ A L C++ G P+P++ W KNG + + ++P+ L +G+L V + D+G Y C ARN +G S +L V V + F Q P +V G L C+ G PEP + WR++ L +G + G L++ D GMY C A+N G + S + + V P+FL +P +Q V + F C V G+PPP + W KE + G+ + D +L I V ED G Y C A + G A L V PP + P +Q C+ GNP P+I W K +Q L+ +S R + G L I ++ D Y C A S +G A L ++
Sbjct: 68 EQPPDLLVSRGEPATLPCRA-EGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLKEEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGE------LPTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHV-----PPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIK 442 HSP 3 Score: 103.219 bits (256), Expect = 4.852e-21 Identity = 88/299 (29.43%), Postives = 131/299 (43.81%), Query Frame = 0
Query: 102 NVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWR-------------RENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
+ P +++P D+ G TL C G P P++ W R + L LP G+ I+ +A P DEG+Y+C A N +G S++ L V F P + V + A ECV G+P PS+ W K+G + G++ + + G L++ K D G YVC A ++AG + A EV +E P P NQ + + C+ G+P P + W K L + R I + +L I ++ ED YTC A + G S SLSV P
Sbjct: 61 DAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVHGRRARP-DEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGAR----LKEEEGRITI-RGGKLMMSHTLKSDAGMYVCVASNMAGERESAA-AEVMVLER---PSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGEL---PTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVP 346
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|49036501|sp|Q9Z2I4.2|ROBO3_MOUSE (RecName: Full=Roundabout homolog 3; AltName: Full=Retinoblastoma-inhibiting gene 1 protein; Short=Rig-1; Flags: Precursor) HSP 1 Score: 391.349 bits (1004), Expect = 2.329e-116 Identity = 266/774 (34.37%), Postives = 403/774 (52.07%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKGSQVDKG--ILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTT---HGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIE-DQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPK----IVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLE-----EVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLS--PPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTM--DPAYIKSPVVSH--PQDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQ 759
L DDFR+ P + +A G+ AV++C P+G+P+P V+W K GK+ +L++E R+T+ G L++ K+D G Y+C A NM G R SG L V +P F++RP + + V +C V GDP+P+L WR+ +D LP G + L I + DEG Y+C A N VG+ S L+VH P F+ KP++Q V F+C GNPPP+IFW KEG+Q L+F + G++ VS G L I V+ DGG+YVC A+SVAGS LAKA LE+ D PPII GPANQTL + + LPC+ GNP+P+I W K+++ L + S+ + +GTL I +++ D Y+C A S GE +W++ L ++ + +P P+T P P P + + S+T++W+ N Q+G + Y +E FS W A + ETYT++ L+P++ Y+FLVRA + G+S PS +SE V+T D L+ + Q L + V + + T++VSW V + V+GF V +R ++G Q ++ + S V+ L + ++ + + G S + + PS PP V + ++ +T+S PP P RNG++ YQ+ GN S FH N + S L + Y +A T AG+G S PV + PA P VS + + ++ +P FL S+ L+L F A +YRRQ
Sbjct: 164 LRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTW-KKGKI--KLKEEEGRITI-RGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRK-DDGELPAGRYEIRSDHSLWIDQVSSEDEGTYTCVAENSVGRAEASGSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPMGRLLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIKGASIDGLPPIILQGPANQTLVLGSSVWLPCRVIGNPQPNIQWKKDERWLQG-DDSQFNLMDNGTLHIASIQEMDMGFYSCVAKSSIGEATWNSWLRKQE------DWGASPGPATGPSNPPGPPSQPIVTEVTANSITLTWKPNPQSGAT-ATSYVIEAFSQAAGNTWRTVADGVQLETYTISGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVQT------QDSSLSRPAEDPWKGQRGLAEVAVRMQEPTVLGPRTLQVSWTVDGPVQLVQGFRVSWR-IAGLDQGSWTMLDLQSPHKQSTVLRGLPPGAQIQIKVQVQGQEGLGAESPFVTRSIPEEAPSGPPQGVAVALGGDRNSSVTVSWEPPLPSQRNGVITEYQIWCLGNESRFHLNRSAAGWARSVTFSGLLPGQIYRALVAAATSAGVGVASAPVLVQLPFPPAAEPGPEVSEGLAERLAKVLRKPAFLAGSSAACGALLLGFCA-ALYRRQ 916 HSP 2 Score: 150.984 bits (380), Expect = 1.006e-35 Identity = 117/387 (30.23%), Postives = 176/387 (45.48%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNG-KVLHELEDEPKRVTLTETGSLVIRDAV-----KADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRREN-DLALPKGS-QVDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES----SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVE 382
E+P D +++G+ A L C++ G P+P++ W KNG +V ED L +G+L V + D+G Y C ARN +G S +L V V + F Q P +V G + C+ G PEP + W++ L +G + G L++ D GMY C A+N G + S + L V P+FL +P +Q V + F C V G+P P++ W K+ + G+ + D +L I V ED G Y C A + G A L V PP P NQT+ C+ GNP P+I W K +Q L+ +S R+++ G L I +++ D Y C A S +G A L ++
Sbjct: 68 EQPPDLVVSRGEPATLPCRA-EGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRSRPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKKGKIKLKEEEGRITIRGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRKDDGE------LPAGRYEIRSDHSLWIDQVSSEDEGTYTCVAENSVGRAEASGSLSVHV-----PPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPMGRLLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIK 442 HSP 3 Score: 99.7525 bits (247), Expect = 5.647e-20 Identity = 87/299 (29.10%), Postives = 135/299 (45.15%), Query Frame = 0
Query: 102 NVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWR-------------RENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDP 384
+ P +++P D+ G TL C G P P++ W R + L LP G+ I+ ++ P DEG+Y+C A N +G S++ L V F P + V + A ECV G+P P + W K+G +L G++ + + G L++ K D G Y+C A ++AG + A E+ +E P P NQ + + C+ G+P+P+++W K+ L A R I + +L ID + ED YTC A + G S SLSV P
Sbjct: 61 DAMPRIVEQPPDLVVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRSRP-DEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTW-KKGKIKLK---EEEGRITI-RGGKLMMSHTFKSDAGMYMCVASNMAGERESGA-AELVVLER---PSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRKDDGELPA---GRYEIRSDHSLWIDQVSSEDEGTYTCVAENSVGRAEASGSLSVHVP 346
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|81872103|sp|Q63155.2|DCC_RAT (RecName: Full=Netrin receptor DCC; AltName: Full=Tumor suppressor protein DCC; Flags: Precursor) HSP 1 Score: 205.297 bits (521), Expect = 7.848e-53 Identity = 201/717 (28.03%), Postives = 307/717 (42.82%), Query Frame = 0
Query: 11 FREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAV-----KADQGDYVCQAR-NMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRR-ENDL-ALPKGSQV---DKGILVIPKAEPHDEGMYSCEATNPV----GKVSQSVVLT---VHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRIT---SETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKV-LISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNID----SYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGP 701
F EP D +G +L C + P + W K+G +L D+ R GSL+I++ + K D+G Y C+A G+ S + V F+ + E +T+ G V L C V GDP P + W++ + DL +P S+V G L I + +P D G+Y C A NP G ++ +L+ +H FL +P + A EC V G PPPS W + G + + + + S L+I V +D G Y C + A A L V PP P+N + C SG P P++NW KN + S I L I + D Y C A +E+G SA L V P P S P P V S V +SWR + + + +TV +FS E + AL T S TV NLKP++ Y F V A N G P + S+ ++ E+Q + VE + + S T+I ++W+ ++ V+G+ + ++S G K+ NI+ SY + L+KFTEY + + + G ++ + V TL+D PSAPP V E++N S +++ PPP +NG + GY++ + TL + ++ + L QY+ + SA T G GP
Sbjct: 42 FVSEPSDAVTMRGGNVLLNCSAESDRGVPVIKWKKDGLILALGMDD--RKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVMVAGPLRFLSQTESITAFMGDTVLLKCEVIGDPMPTIHWQKNQQDLNPIPGDSRVVVLPSGALQISRLQPGDSGVYRCSARNPASTRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLR-GEEVIQLRSKKYSLLGGSN---LLISNVTDDDSGTYTCVVTYKNENISASAELTVLV-----PPWFLNHPSNLYAYESMDIEFECAVSGKPVPTVNWMKNGD--VVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKPA--------IPSSSILPSAPRDVVPVLVSSRFVRLSWRPPAE-AKGNIQTFTV-FFSREGDNR--ERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWG---PGESSQPIKVA----------TQPELQ---VPGPVENLHAVSASPTSILITWEPPAYANGPVQGYRLFCTEVSTG----------KEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEME-TLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGP 706
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|296434474|sp|P43146.2|DCC_HUMAN (RecName: Full=Netrin receptor DCC; AltName: Full=Colorectal cancer suppressor; AltName: Full=Immunoglobulin superfamily DCC subclass member 1; AltName: Full=Tumor suppressor protein DCC; Flags: Precursor) HSP 1 Score: 204.912 bits (520), Expect = 1.110e-52 Identity = 202/717 (28.17%), Postives = 306/717 (42.68%), Query Frame = 0
Query: 11 FREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAV-----KADQGDYVCQAR-NMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRR-ENDLA-LPKGSQV---DKGILVIPKAEPHDEGMYSCEATNPV----GKVSQSVVLT---VHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRIT---SETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKV-LISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNID----SYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGP 701
F EP D +G +L C + P + W K+G L DE R GSL+I++ + K D+G Y C+A G+ S + V F+ + E VT+ G V L C V G+P P + W++ + DL +P S+V G L I + +P D G+Y C A NP G ++ +L+ +H FL +P + A EC V G PPPS W + G + + + + S L+I V +D G Y C + A A L V PP P+N + C SG P P++NW KN + S I L I + D Y C A +E+G SA L V P P S P P V S V +SWR + + + +TV +FS E + AL T S TV NLKP++ Y F V A N G P + S+ ++ E+Q + VE + + S T+I ++W+ ++ V+G+ + ++S G K+ NI+ SY + L+KFTEY + + + G ++ + V TL+D PSAPP V E++N S +++ PPP +NG + GY++ + TL + ++ + L QY+ + SA T G GP
Sbjct: 42 FLSEPSDAVTMRGGNVLLDCSAESDRGVPVIKWKKDGIHLALGMDE--RKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPLRFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLR-GEEVIQLRSKKYSLLGGSN---LLISNVTDDDSGMYTCVVTYKNENISASAELTVLV-----PPWFLNHPSNLYAYESMDIEFECTVSGKPVPTVNWMKNGD--VVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQTSAQLIVPKPA--------IPSSSVLPSAPRDVVPVLVSSRFVRLSWRPPAE-AKGNIQTFTV-FFSREGDNR--ERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWG---PGESSQPIKVA----------TQPELQ---VPGPVENLQAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTG----------KEQNIEVDGLSYKLEGLKKFTEYSLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEME-TLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGP 706
BLAST of conserved hypothetical protein vs. nr
Match: gi|1339093992|ref|XP_023708253.1| (protein sax-3-like isoform X2 [Cryptotermes secundus]) HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0 Identity = 453/1001 (45.25%), Postives = 620/1001 (61.94%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN-DLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQT--GQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYI-KSPVVSHPQDVGD---LISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHWKNGSDLVVQGQKSGEC----FYNDNSDGQAL------YAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTL--AMQNRIRSVHGKTFMALPQHDPSSQDFLSHKTTSSSGGDSLSNGQKSP--------------GGDF---------------SAGKRTGSSSGHSGGGGNFY--------LPNWSELF 951
L D+FR P DTR+A G+TA+L+C P+G+P+PS+ W KNG+ ++ + KRV + + G+L+I D D+G Y C A+NMVG R S P TL V+VKP F + P DVT G V C V GDP+P+++WRR++ + + + +D L I EP DEG+Y C+A N VG VS LTVH+ PTF KP+DQ+VG+NG+A FECV GNPPPS+FWTKEG+Q LMF G ++G +HV+ +GTL IQGV++ED GF VCSALSVAGST +A+L+VT+++D PPPI+ +GPANQTLP+ ++A LPCQA G P+P I W+KN PL + + RI + GTL ID+L++ D+ LYTCTA SESGETSWSASL+VE PN+ H+ PDPST+P P +P I+N +SVTISW ++ G SPLIGYT+EYFS +++TGWVVAA R+T++T T+ +LKPD+SYVFLVRAENSHG+S PS +S+ RTL A + L+E + L KVVEL ++ ISST++R+ W +L+ YVEGF++RFRD+SGGSQ++NM TV+ + Y + NLRKFT+YE FL PFFK+IEGQPSNS VQT D PSAPP V +LN T+A + SPPPPQH NGIL GY++ +KGN S + +TLNATT +L+NL+ YT R A+T GLGP S PV MDP+ + + P +HP + GD ++ E WFL+ + V+ L+L FV ++ RR+ + K LGHL VPV D+S+L+ + ++W++ W+ ++ SG C N+ + Q L YAEV DT + T ++ T + D PAPYATTTL +M R + F+ + P + + ++ S LS+ +P G + AG R S+ H+ LPNW+E
Sbjct: 8 LRDEFRPMPKDTRVAAGETALLECGPPKGHPQPSLVWRKNGQTINI--EASKRVRVVDGGNLMIADVRPGDEGKYQCVAQNMVGIRESDPATLTVHVKPFFSKEPSDVTVLKGQSVQFQCRVGGDPDPNILWRRDDGKMPIGRAQILDDKSLRIENVEPVDEGLYICDAENLVGSVSARASLTVHSPPTFTTKPQDQKVGLNGLASFECVATGNPPPSVFWTKEGSQVLMFPGNSYGHLHVTPEGTLKIQGVQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVQIGPANQTLPLQSVATLPCQAIGTPQPKIKWYKNGSPLIS-QGPRIKVMDPGTLHIDDLQLTDSGLYTCTASSESGETSWSASLTVEKTAAPNL--HRMPDPSTYPSAPPQPHILNTTQSSVTISWHEATKSKPGASPLIGYTIEYFSSDLQTGWVVAANRVTADTITIGDLKPDTSYVFLVRAENSHGLSVPSPVSDVARTLGADRRAVPQHELDEARARLGTKVVELRDVQPISSTSVRLIWDILVGEAYVEGFYIRFRDLSGGSQQYNMVTVLNAGST-GYTVANLRKFTKYEFFLVPFFKSIEGQPSNSKIVQTAEDVPSAPPGNVQVGMLNATAAFVRWSPPPPQHHNGILLGYKIQIKGNSSKILAQMTLNATTTSVLLNNLTTGGSYTARVVAYTHVGLGPLSAPVPLVMDPSLLHQYPPRAHPSEGGDGSSVVRETWFLLLIGGTVLTLVLGFVGMLYLRRRQALSKE-LGHLNVPVVNANDISQLN--LMNGKETLWID-------RGWRPANE-----DSSGICVETKLLNNQATNQDLNSSATDYAEV-DTRNLTTFYSNCRTKEPDI----PAPYATTTLINSMHRRDNMDNSHLFVPIAMGAPGGGEAKTSSSSDSCVKPDLSSLDTNPELGNKSSSSPSSEIGSVYIDDSGLPMRRVQPASVAGCRKFSAPSHTAVNSGGGQQAQGQTALPNWAEFL 982
BLAST of conserved hypothetical protein vs. nr
Match: gi|1339093990|ref|XP_023708251.1| (protein sax-3-like isoform X1 [Cryptotermes secundus]) HSP 1 Score: 828.935 bits (2140), Expect = 0.000e+0 Identity = 453/1005 (45.07%), Postives = 620/1005 (61.69%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN-DLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQT--GQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYI-KSPVVSHPQDVGD---LISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHWKNGSDLVVQGQKSGEC----FYNDNSDGQAL------YAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTL--AMQNRIRSVHGKTFMALPQHDPSSQDFLSHKTTSSSGGDSLSNGQKSP--------------GGDF-------------------SAGKRTGSSSGHSGGGGNFY--------LPNWSELF 951
L D+FR P DTR+A G+TA+L+C P+G+P+PS+ W KNG+ ++ + KRV + + G+L+I D D+G Y C A+NMVG R S P TL V+VKP F + P DVT G V C V GDP+P+++WRR++ + + + +D L I EP DEG+Y C+A N VG VS LTVH+ PTF KP+DQ+VG+NG+A FECV GNPPPS+FWTKEG+Q LMF G ++G +HV+ +GTL IQGV++ED GF VCSALSVAGST +A+L+VT+++D PPPI+ +GPANQTLP+ ++A LPCQA G P+P I W+KN PL + + RI + GTL ID+L++ D+ LYTCTA SESGETSWSASL+VE PN+ H+ PDPST+P P +P I+N +SVTISW ++ G SPLIGYT+EYFS +++TGWVVAA R+T++T T+ +LKPD+SYVFLVRAENSHG+S PS +S+ RTL A + L+E + L KVVEL ++ ISST++R+ W +L+ YVEGF++RFRD+SGGSQ++NM TV+ + Y + NLRKFT+YE FL PFFK+IEGQPSNS VQT D PSAPP V +LN T+A + SPPPPQH NGIL GY++ +KGN S + +TLNATT +L+NL+ YT R A+T GLGP S PV MDP+ + + P +HP + GD ++ E WFL+ + V+ L+L FV ++ RR+ + K LGHL VPV D+S+L+ + ++W++ W+ ++ SG C N+ + Q L YAEV DT + T ++ T + D PAPYATTTL +M R + F+ + P + + ++ S LS+ +P G + AG R S+ H+ LPNW+E
Sbjct: 8 LRDEFRPMPKDTRVAAGETALLECGPPKGHPQPSLVWRKNGQTINI--EASKRVRVVDGGNLMIADVRPGDEGKYQCVAQNMVGIRESDPATLTVHVKPFFSKEPSDVTVLKGQSVQFQCRVGGDPDPNILWRRDDGKMPIGRAQILDDKSLRIENVEPVDEGLYICDAENLVGSVSARASLTVHSPPTFTTKPQDQKVGLNGLASFECVATGNPPPSVFWTKEGSQVLMFPGNSYGHLHVTPEGTLKIQGVQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVQIGPANQTLPLQSVATLPCQAIGTPQPKIKWYKNGSPLIS-QGPRIKVMDPGTLHIDDLQLTDSGLYTCTASSESGETSWSASLTVEKTAAPNL--HRMPDPSTYPSAPPQPHILNTTQSSVTISWHEATKSKPGASPLIGYTIEYFSSDLQTGWVVAANRVTADTITIGDLKPDTSYVFLVRAENSHGLSVPSPVSDVARTLGADRRAVPQHELDEARARLGTKVVELRDVQPISSTSVRLIWDILVGEAYVEGFYIRFRDLSGGSQQYNMVTVLNAGST-GYTVANLRKFTKYEFFLVPFFKSIEGQPSNSKIVQTAEDVPSAPPGNVQVGMLNATAAFVRWSPPPPQHHNGILLGYKIQIKGNSSKILAQMTLNATTTSVLLNNLTTGGSYTARVVAYTHVGLGPLSAPVPLVMDPSLLHQYPPRAHPSEGGDGSSVVRETWFLLLIGGTVLTLVLGFVGMLYLRRRQALSKE-LGHLNVPVVNANDISQLN--LMNGKETLWID-------RGWRPANE-----DSSGICVETKLLNNQATNQDLNSSATDYAEV-DTRNLTTFYSNCRTKEPDI----PAPYATTTLINSMHRRDNMDNSHLFVPIAMGAPGGGEAKTSSSSDSCVKPDLSSLDTNPELGNKSSSSPSSEIGSVYIVCVPDDSGLPMRRVQPASVAGCRKFSAPSHTAVNSGGGQQAQGQTALPNWAEFL 986
BLAST of conserved hypothetical protein vs. nr
Match: gi|1339093996|ref|XP_023708255.1| (protein sax-3-like isoform X4 [Cryptotermes secundus]) HSP 1 Score: 828.55 bits (2139), Expect = 0.000e+0 Identity = 453/1005 (45.07%), Postives = 620/1005 (61.69%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN-DLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQT--GQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYI-KSPVVSHPQDVGD---LISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHWKNGSDLVVQGQKSGEC----FYNDNSDGQAL------YAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTL--AMQNRIRSVHGKTFMALPQHDPSSQDFLSHKTTSSSGGDSLSNGQKSP--------------GGDF-------------------SAGKRTGSSSGHSGGGGNFY--------LPNWSELF 951
L D+FR P DTR+A G+TA+L+C P+G+P+PS+ W KNG+ ++ + KRV + + G+L+I D D+G Y C A+NMVG R S P TL V+VKP F + P DVT G V C V GDP+P+++WRR++ + + + +D L I EP DEG+Y C+A N VG VS LTVH+ PTF KP+DQ+VG+NG+A FECV GNPPPS+FWTKEG+Q LMF G ++G +HV+ +GTL IQGV++ED GF VCSALSVAGST +A+L+VT+++D PPPI+ +GPANQTLP+ ++A LPCQA G P+P I W+KN PL + + RI + GTL ID+L++ D+ LYTCTA SESGETSWSASL+VE PN+ H+ PDPST+P P +P I+N +SVTISW ++ G SPLIGYT+EYFS +++TGWVVAA R+T++T T+ +LKPD+SYVFLVRAENSHG+S PS +S+ RTL A + L+E + L KVVEL ++ ISST++R+ W +L+ YVEGF++RFRD+SGGSQ++NM TV+ + Y + NLRKFT+YE FL PFFK+IEGQPSNS VQT D PSAPP V +LN T+A + SPPPPQH NGIL GY++ +KGN S + +TLNATT +L+NL+ YT R A+T GLGP S PV MDP+ + + P +HP + GD ++ E WFL+ + V+ L+L FV ++ RR+ + K LGHL VPV D+S+L+ + ++W++ W+ ++ SG C N+ + Q L YAEV DT + T ++ T + D PAPYATTTL +M R + F+ + P + + ++ S LS+ +P G + AG R S+ H+ LPNW+E
Sbjct: 8 LRDEFRPMPKDTRVAAGETALLECGPPKGHPQPSLVWRKNGQTINI--EASKRVRVVDGGNLMIADVRPGDEGKYQCVAQNMVGIRESDPATLTVHVKPFFSKEPSDVTVLKGQSVQFQCRVGGDPDPNILWRRDDGKMPIGRAQILDDKSLRIENVEPVDEGLYICDAENLVGSVSARASLTVHSPPTFTTKPQDQKVGLNGLASFECVATGNPPPSVFWTKEGSQVLMFPGNSYGHLHVTPEGTLKIQGVQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVQIGPANQTLPLQSVATLPCQAIGTPQPKIKWYKNGSPLIS-QGPRIKVMDPGTLHIDDLQLTDSGLYTCTASSESGETSWSASLTVEKTAAPNL--HRMPDPSTYPSAPPQPHILNTTQSSVTISWHEATKSKPGASPLIGYTIEYFSSDLQTGWVVAANRVTADTITIGDLKPDTSYVFLVRAENSHGLSVPSPVSDVARTLGADRRAVPQHELDEARARLGTKVVELRDVQPISSTSVRLIWDILVGEAYVEGFYIRFRDLSGGSQQYNMVTVLNAGST-GYTVANLRKFTKYEFFLVPFFKSIEGQPSNSKIVQTAEDVPSAPPGNVQVGMLNATAAFVRWSPPPPQHHNGILLGYKIQIKGNSSKILAQMTLNATTTSVLLNNLTTGGSYTARVVAYTHVGLGPLSAPVPLVMDPSLLHQYPPRAHPSEGGDGSSVVRETWFLLLIGGTVLTLVLGFVGMLYLRRRQALSKE-LGHLNVPVVNANDISQLN--LMNGKETLWID-------RGWRPANE-----DSSGICVETKLLNNQATNQDLNSSATDYAEV-DTRNLTTFYSNCRTKEPDI----PAPYATTTLINSMHRRDNMDNSHLFVPIAMGAPGGGEAKTSSSSDSCVKPDLSSLDTNPELGNKSSSSPSSEIGSVYIVCVPDDSGLPMRRVQPASVAGCRKFSAPSHTAVNSGGGQQAQGQTALPNWAEFL 986
BLAST of conserved hypothetical protein vs. nr
Match: gi|1339093994|ref|XP_023708254.1| (protein sax-3-like isoform X3 [Cryptotermes secundus]) HSP 1 Score: 828.165 bits (2138), Expect = 0.000e+0 Identity = 453/1005 (45.07%), Postives = 620/1005 (61.69%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN-DLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQT--GQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYI-KSPVVSHPQDVGD---LISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHWKNGSDLVVQGQKSGEC----FYNDNSDGQAL------YAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTL--AMQNRIRSVHGKTFMALPQHDPSSQDFLSHKTTSSSGGDSLSNGQKSP--------------GGDF-------------------SAGKRTGSSSGHSGGGGNFY--------LPNWSELF 951
L D+FR P DTR+A G+TA+L+C P+G+P+PS+ W KNG+ ++ + KRV + + G+L+I D D+G Y C A+NMVG R S P TL V+VKP F + P DVT G V C V GDP+P+++WRR++ + + + +D L I EP DEG+Y C+A N VG VS LTVH+ PTF KP+DQ+VG+NG+A FECV GNPPPS+FWTKEG+Q LMF G ++G +HV+ +GTL IQGV++ED GF VCSALSVAGST +A+L+VT+++D PPPI+ +GPANQTLP+ ++A LPCQA G P+P I W+KN PL + + RI + GTL ID+L++ D+ LYTCTA SESGETSWSASL+VE PN+ H+ PDPST+P P +P I+N +SVTISW ++ G SPLIGYT+EYFS +++TGWVVAA R+T++T T+ +LKPD+SYVFLVRAENSHG+S PS +S+ RTL A + L+E + L KVVEL ++ ISST++R+ W +L+ YVEGF++RFRD+SGGSQ++NM TV+ + Y + NLRKFT+YE FL PFFK+IEGQPSNS VQT D PSAPP V +LN T+A + SPPPPQH NGIL GY++ +KGN S + +TLNATT +L+NL+ YT R A+T GLGP S PV MDP+ + + P +HP + GD ++ E WFL+ + V+ L+L FV ++ RR+ + K LGHL VPV D+S+L+ + ++W++ W+ ++ SG C N+ + Q L YAEV DT + T ++ T + D PAPYATTTL +M R + F+ + P + + ++ S LS+ +P G + AG R S+ H+ LPNW+E
Sbjct: 8 LRDEFRPMPKDTRVAAGETALLECGPPKGHPQPSLVWRKNGQTINI--EASKRVRVVDGGNLMIADVRPGDEGKYQCVAQNMVGIRESDPATLTVHVKPFFSKEPSDVTVLKGQSVQFQCRVGGDPDPNILWRRDDGKMPIGRAQILDDKSLRIENVEPVDEGLYICDAENLVGSVSARASLTVHSPPTFTTKPQDQKVGLNGLASFECVATGNPPPSVFWTKEGSQVLMFPGNSYGHLHVTPEGTLKIQGVQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVQIGPANQTLPLQSVATLPCQAIGTPQPKIKWYKNGSPLIS-QGPRIKVMDPGTLHIDDLQLTDSGLYTCTASSESGETSWSASLTVEKTAAPNL--HRMPDPSTYPSAPPQPHILNTTQSSVTISWHEATKSKPGASPLIGYTIEYFSSDLQTGWVVAANRVTADTITIGDLKPDTSYVFLVRAENSHGLSVPSPVSDVARTLGADRRAVPQHELDEARARLGTKVVELRDVQPISSTSVRLIWDILVGEAYVEGFYIRFRDLSGGSQQYNMVTVLNAGST-GYTVANLRKFTKYEFFLVPFFKSIEGQPSNSKIVQTAEDVPSAPPGNVQVGMLNATAAFVRWSPPPPQHHNGILLGYKIQIKGNSSKILAQMTLNATTTSVLLNNLTTGGSYTARVVAYTHVGLGPLSAPVPLVMDPSLLHQYPPRAHPSEGGDGSSVVRETWFLLLIGGTVLTLVLGFVGMLYLRRRQALSKE-LGHLNVPVVNANDISQLN--LMNGKETLWID-------RGWRPANE-----DSSGICVETKLLNNQATNQDLNSSATDYAEV-DTRNLTTFYSNCRTKEPDI----PAPYATTTLINSMHRRDNMDNSHLFVPIAMGAPGGGEAKTSSSSDSCVKPDLSSLDTNPELGNKSSSSPSSEIGSVYIVCVPDDSGLPMRRVQPASVAGCRKFSAPSHTAVNSGGGQQAQGQTALPNWAEFL 986
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238832918|ref|XP_022253067.1| (roundabout homolog 1-like isoform X2 [Limulus polyphemus]) HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0 Identity = 427/892 (47.87%), Postives = 563/892 (63.12%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKG-SQVDKGI-LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHP------QDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHW-----KNGSDLVVQGQKSGECFYND---NSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLAMQNRIRSVHG 882
L DDFR P D ++A+ D+A L+C PRG+P P VSW KNG ++ E RV +T +G+LVI D + DQG YVC A NM G RNS P L V VKP FI+ PEDV + V + CLV GDP P + W R D +P G +Q+ K L I + DEG+Y CEA N VG S S LTVH+ P+F VKPK+QRVG+NGIAKFECV GNPPPSIFWTKEGNQ LMF ++G+ V+ GTL+I GV KED G+YVCSALSV ST+ KA+L+V A+ D PPPII +GPANQTLP NT+A+LPC+A G P P+I WF + V + R I SGTL ID+L+ D+ LYTCTA SESGETSWSA+LSVE P+NPN IFH+ DPSTFP PP KP +N T++T+ W R+ + G SPLIGYTVEY+S +++ GW+VAA R+ E++ V+NL+PDS YVFLVRAENSHG+S PS++S+ + TL + + +LEE + +L +V+L AISST +++ W++ + YVEGF++RFRD SG SQK+NM TV+ SYV+T+LRKFT+YE FL PF+K++EG PSNS QTL D PSAPP + ++ N+T A I+ S PPPQ+RNGILQGY +H+ N S HSN+T N TT + NL+ +Y+++A AFT G GPFS PV MD +Y+ S P + D + +PWF+ + I+ +L+ IF ++ R+ + K+ HL V + ED+ A S ++W+N H W K G ++ + E ND S L EV +D + T S+ PAPYATTTL + ++ G
Sbjct: 129 LRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGL---PVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRR-DGKMPIGRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHSQPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQNTMAMLPCEAEGIPGPNIQWFFKSASV-DVNNPRFSILDSGTLQIDDLQPLDSGLYTCTASSESGETSWSATLSVESPRNPNAIFHRMFDPSTFPGPPGKPTAINVTETAITLRWLRSSKLGDSPLIGYTVEYYSSDLKRGWMVAARRVKRESFVVHNLRPDSHYVFLVRAENSHGLSVPSEISDVICTLGLPSHILPEYDLEEARYYLSTSIVKLKDARAISSTAVKLMWEIQSNLGYVEGFYIRFRDSSGRSQKYNMITVL-NGAATSYVLTDLRKFTKYEFFLVPFYKSVEGPPSNSRKAQTLEDVPSAPPDNLNVKLKNLTVAVISWSSPPPQYRNGILQGYNIHILKNTSQLHSNITTNGTTTLITVYNLTAGHKYSVKAVAFTSVGNGPFSAPVALVMDSSYLNDQHDSGPASTSLTSSIHDTLQQPWFIALIGGILCLLLSIFFIVLFLCRRMAWKKALEAHLPVAAHKSEDVR----AGVSNHEALWIN-------HTWHPTDGKTGQNISESKLLNKETSANDLNYASVYSPLQYEVNGSDYAEVDSHSMTTFYKKGSSSVPAPYATTTLINPSSYKTCTG 1003 HSP 2 Score: 157.532 bits (397), Expect = 3.127e-35 Identity = 125/406 (30.79%), Postives = 177/406 (43.60%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSL------VIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD---KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES-SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKN-PNIIFHKTPDPSTFPQ 402
E P D + + A L C++ G P P + W NGK++ E R+ L + V+ D G Y C ARN +G S TL V + + F P D+ TL C G P+P + W + +G +V G LVI D+G Y C A N G + S +L+V P F+ +P+D N C+VDG+P P++ W + + + G+ + ++ +L I VR ED G Y+C A + G A L V + P A+ P NQ + +N IA C ASGNP PSI W K NQ ++ S R + + GTL+I + ED Y C+A S T A L V + P I P T PQ
Sbjct: 36 EHPRDLLVKKQQPAKLNCKA-DGVPTPVIEWYHNGKLV---EGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRRDGKMPI------GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHS-----QPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQ 426 HSP 3 Score: 94.3597 bits (233), Expect = 7.744e-16 Identity = 84/295 (28.47%), Postives = 128/295 (43.39%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE--------NDLALPKGSQVDKGIL-VIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG-STLAKAYLEVTA---IEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKN--QQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
P + P D+ K L C G P P + W N + L G V L V+ D G Y C A N +G+ S++ L V F P+D +V A EC G+P P + W K G + + G++ ++ G LVI+ VR+ D G YVC A ++AG + A L V +P ++A+ +NQ + + C G+P P++ W + + P+ R I+ + +L I +R ED LY C A + G S SA+L+V
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGKLVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNG----LPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLAL--SNQNVDI------ACLVDGDPTPTVTWHRRDGKMPI-----GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTV 309 HSP 4 Score: 77.7962 bits (190), Expect = 9.851e-11 Identity = 63/198 (31.82%), Postives = 87/198 (43.94%), Query Frame = 0
Query: 193 PTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTL-----VIQGVRKEDGGFYVCSALSVAGSTLAK-AYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQ-ASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESG-ETSWSASLSVE 382
P P+D V AK C DG P P I W G + GT++ M V+ G V+ G R +D G Y C A + G ++ A LEV + D P + + A L C G+P P ++W KN P+ + E R+ I SG LVI+++R D Y C A + +G S A LSV
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGK---LVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDD----FRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDS-EGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVR 221
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238832916|ref|XP_022253064.1| (roundabout homolog 1-like isoform X1 [Limulus polyphemus]) HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0 Identity = 427/892 (47.87%), Postives = 563/892 (63.12%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKG-SQVDKGI-LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHP------QDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHW-----KNGSDLVVQGQKSGECFYND---NSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLAMQNRIRSVHG 882
L DDFR P D ++A+ D+A L+C PRG+P P VSW KNG ++ E RV +T +G+LVI D + DQG YVC A NM G RNS P L V VKP FI+ PEDV + V + CLV GDP P + W R D +P G +Q+ K L I + DEG+Y CEA N VG S S LTVH+ P+F VKPK+QRVG+NGIAKFECV GNPPPSIFWTKEGNQ LMF ++G+ V+ GTL+I GV KED G+YVCSALSV ST+ KA+L+V A+ D PPPII +GPANQTLP NT+A+LPC+A G P P+I WF + V + R I SGTL ID+L+ D+ LYTCTA SESGETSWSA+LSVE P+NPN IFH+ DPSTFP PP KP +N T++T+ W R+ + G SPLIGYTVEY+S +++ GW+VAA R+ E++ V+NL+PDS YVFLVRAENSHG+S PS++S+ + TL + + +LEE + +L +V+L AISST +++ W++ + YVEGF++RFRD SG SQK+NM TV+ SYV+T+LRKFT+YE FL PF+K++EG PSNS QTL D PSAPP + ++ N+T A I+ S PPPQ+RNGILQGY +H+ N S HSN+T N TT + NL+ +Y+++A AFT G GPFS PV MD +Y+ S P + D + +PWF+ + I+ +L+ IF ++ R+ + K+ HL V + ED+ A S ++W+N H W K G ++ + E ND S L EV +D + T S+ PAPYATTTL + ++ G
Sbjct: 129 LRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGL---PVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRR-DGKMPIGRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHSQPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQNTMAMLPCEAEGIPGPNIQWFFKSASV-DVNNPRFSILDSGTLQIDDLQPLDSGLYTCTASSESGETSWSATLSVESPRNPNAIFHRMFDPSTFPGPPGKPTAINVTETAITLRWLRSSKLGDSPLIGYTVEYYSSDLKRGWMVAARRVKRESFVVHNLRPDSHYVFLVRAENSHGLSVPSEISDVICTLGLPSHILPEYDLEEARYYLSTSIVKLKDARAISSTAVKLMWEIQSNLGYVEGFYIRFRDSSGRSQKYNMITVL-NGAATSYVLTDLRKFTKYEFFLVPFYKSVEGPPSNSRKAQTLEDVPSAPPDNLNVKLKNLTVAVISWSSPPPQYRNGILQGYNIHILKNTSQLHSNITTNGTTTLITVYNLTAGHKYSVKAVAFTSVGNGPFSAPVALVMDSSYLNDQHDSGPASTSLTSSIHDTLQQPWFIALIGGILCLLLSIFFIVLFLCRRMAWKKALEAHLPVAAHKSEDVR----AGVSNHEALWIN-------HTWHPTDGKTGQNISESKLLNKETSANDLNYASVYSPLQYEVNGSDYAEVDSHSMTTFYKKGSSSVPAPYATTTLINPSSYKTCTG 1003 HSP 2 Score: 157.532 bits (397), Expect = 3.102e-35 Identity = 125/406 (30.79%), Postives = 177/406 (43.60%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSL------VIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD---KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES-SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKN-PNIIFHKTPDPSTFPQ 402
E P D + + A L C++ G P P + W NGK++ E R+ L + V+ D G Y C ARN +G S TL V + + F P D+ TL C G P+P + W + +G +V G LVI D+G Y C A N G + S +L+V P F+ +P+D N C+VDG+P P++ W + + + G+ + ++ +L I VR ED G Y+C A + G A L V + P A+ P NQ + +N IA C ASGNP PSI W K NQ ++ S R + + GTL+I + ED Y C+A S T A L V + P I P T PQ
Sbjct: 36 EHPRDLLVKKQQPAKLNCKA-DGVPTPVIEWYHNGKLV---EGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRRDGKMPI------GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHS-----QPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQ 426 HSP 3 Score: 94.7449 bits (234), Expect = 7.551e-16 Identity = 84/295 (28.47%), Postives = 128/295 (43.39%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE--------NDLALPKGSQVDKGIL-VIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG-STLAKAYLEVTA---IEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKN--QQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
P + P D+ K L C G P P + W N + L G V L V+ D G Y C A N +G+ S++ L V F P+D +V A EC G+P P + W K G + + G++ ++ G LVI+ VR+ D G YVC A ++AG + A L V +P ++A+ +NQ + + C G+P P++ W + + P+ R I+ + +L I +R ED LY C A + G S SA+L+V
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGKLVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNG----LPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLAL--SNQNVDI------ACLVDGDPTPTVTWHRRDGKMPI-----GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTV 309 HSP 4 Score: 77.7962 bits (190), Expect = 9.770e-11 Identity = 63/198 (31.82%), Postives = 87/198 (43.94%), Query Frame = 0
Query: 193 PTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTL-----VIQGVRKEDGGFYVCSALSVAGSTLAK-AYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQ-ASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESG-ETSWSASLSVE 382
P P+D V AK C DG P P I W G + GT++ M V+ G V+ G R +D G Y C A + G ++ A LEV + D P + + A L C G+P P ++W KN P+ + E R+ I SG LVI+++R D Y C A + +G S A LSV
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGK---LVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDD----FRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDS-EGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVR 221
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238832920|ref|XP_022253073.1| (roundabout homolog 1-like isoform X3 [Limulus polyphemus]) HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0 Identity = 427/892 (47.87%), Postives = 563/892 (63.12%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKG-SQVDKGI-LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHP------QDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHW-----KNGSDLVVQGQKSGECFYND---NSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLAMQNRIRSVHG 882
L DDFR P D ++A+ D+A L+C PRG+P P VSW KNG ++ E RV +T +G+LVI D + DQG YVC A NM G RNS P L V VKP FI+ PEDV + V + CLV GDP P + W R D +P G +Q+ K L I + DEG+Y CEA N VG S S LTVH+ P+F VKPK+QRVG+NGIAKFECV GNPPPSIFWTKEGNQ LMF ++G+ V+ GTL+I GV KED G+YVCSALSV ST+ KA+L+V A+ D PPPII +GPANQTLP NT+A+LPC+A G P P+I WF + V + R I SGTL ID+L+ D+ LYTCTA SESGETSWSA+LSVE P+NPN IFH+ DPSTFP PP KP +N T++T+ W R+ + G SPLIGYTVEY+S +++ GW+VAA R+ E++ V+NL+PDS YVFLVRAENSHG+S PS++S+ + TL + + +LEE + +L +V+L AISST +++ W++ + YVEGF++RFRD SG SQK+NM TV+ SYV+T+LRKFT+YE FL PF+K++EG PSNS QTL D PSAPP + ++ N+T A I+ S PPPQ+RNGILQGY +H+ N S HSN+T N TT + NL+ +Y+++A AFT G GPFS PV MD +Y+ S P + D + +PWF+ + I+ +L+ IF ++ R+ + K+ HL V + ED+ A S ++W+N H W K G ++ + E ND S L EV +D + T S+ PAPYATTTL + ++ G
Sbjct: 129 LRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGL---PVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRR-DGKMPIGRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHSQPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQNTMAMLPCEAEGIPGPNIQWFFKSASV-DVNNPRFSILDSGTLQIDDLQPLDSGLYTCTASSESGETSWSATLSVESPRNPNAIFHRMFDPSTFPGPPGKPTAINVTETAITLRWLRSSKLGDSPLIGYTVEYYSSDLKRGWMVAARRVKRESFVVHNLRPDSHYVFLVRAENSHGLSVPSEISDVICTLGLPSHILPEYDLEEARYYLSTSIVKLKDARAISSTAVKLMWEIQSNLGYVEGFYIRFRDSSGRSQKYNMITVL-NGAATSYVLTDLRKFTKYEFFLVPFYKSVEGPPSNSRKAQTLEDVPSAPPDNLNVKLKNLTVAVISWSSPPPQYRNGILQGYNIHILKNTSQLHSNITTNGTTTLITVYNLTAGHKYSVKAVAFTSVGNGPFSAPVALVMDSSYLNDQHDSGPASTSLTSSIHDTLQQPWFIALIGGILCLLLSIFFIVLFLCRRMAWKKALEAHLPVAAHKSEDVR----AGVSNHEALWIN-------HTWHPTDGKTGQNISESKLLNKETSANDLNYASVYSPLQYEVNGSDYAEVDSHSMTTFYKKGSSSVPAPYATTTLINPSSYKTCTG 1003 HSP 2 Score: 157.532 bits (397), Expect = 3.074e-35 Identity = 125/406 (30.79%), Postives = 177/406 (43.60%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSL------VIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD---KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES-SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKN-PNIIFHKTPDPSTFPQ 402
E P D + + A L C++ G P P + W NGK++ E R+ L + V+ D G Y C ARN +G S TL V + + F P D+ TL C G P+P + W + +G +V G LVI D+G Y C A N G + S +L+V P F+ +P+D N C+VDG+P P++ W + + + G+ + ++ +L I VR ED G Y+C A + G A L V + P A+ P NQ + +N IA C ASGNP PSI W K NQ ++ S R + + GTL+I + ED Y C+A S T A L V + P I P T PQ
Sbjct: 36 EHPRDLLVKKQQPAKLNCKA-DGVPTPVIEWYHNGKLV---EGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRRDGKMPI------GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHS-----QPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQ 426 HSP 3 Score: 94.7449 bits (234), Expect = 7.422e-16 Identity = 84/295 (28.47%), Postives = 128/295 (43.39%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE--------NDLALPKGSQVDKGIL-VIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG-STLAKAYLEVTA---IEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKN--QQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
P + P D+ K L C G P P + W N + L G V L V+ D G Y C A N +G+ S++ L V F P+D +V A EC G+P P + W K G + + G++ ++ G LVI+ VR+ D G YVC A ++AG + A L V +P ++A+ +NQ + + C G+P P++ W + + P+ R I+ + +L I +R ED LY C A + G S SA+L+V
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGKLVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNG----LPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLAL--SNQNVDI------ACLVDGDPTPTVTWHRRDGKMPI-----GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTV 309 HSP 4 Score: 77.7962 bits (190), Expect = 9.935e-11 Identity = 63/198 (31.82%), Postives = 87/198 (43.94%), Query Frame = 0
Query: 193 PTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTL-----VIQGVRKEDGGFYVCSALSVAGSTLAK-AYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQ-ASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESG-ETSWSASLSVE 382
P P+D V AK C DG P P I W G + GT++ M V+ G V+ G R +D G Y C A + G ++ A LEV + D P + + A L C G+P P ++W KN P+ + E R+ I SG LVI+++R D Y C A + +G S A LSV
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGK---LVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDD----FRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDS-EGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVR 221
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238832922|ref|XP_022253078.1| (roundabout homolog 1-like isoform X4 [Limulus polyphemus]) HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0 Identity = 427/892 (47.87%), Postives = 563/892 (63.12%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKG-SQVDKGI-LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHP------QDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHW-----KNGSDLVVQGQKSGECFYND---NSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLAMQNRIRSVHG 882
L DDFR P D ++A+ D+A L+C PRG+P P VSW KNG ++ E RV +T +G+LVI D + DQG YVC A NM G RNS P L V VKP FI+ PEDV + V + CLV GDP P + W R D +P G +Q+ K L I + DEG+Y CEA N VG S S LTVH+ P+F VKPK+QRVG+NGIAKFECV GNPPPSIFWTKEGNQ LMF ++G+ V+ GTL+I GV KED G+YVCSALSV ST+ KA+L+V A+ D PPPII +GPANQTLP NT+A+LPC+A G P P+I WF + V + R I SGTL ID+L+ D+ LYTCTA SESGETSWSA+LSVE P+NPN IFH+ DPSTFP PP KP +N T++T+ W R+ + G SPLIGYTVEY+S +++ GW+VAA R+ E++ V+NL+PDS YVFLVRAENSHG+S PS++S+ + TL + + +LEE + +L +V+L AISST +++ W++ + YVEGF++RFRD SG SQK+NM TV+ SYV+T+LRKFT+YE FL PF+K++EG PSNS QTL D PSAPP + ++ N+T A I+ S PPPQ+RNGILQGY +H+ N S HSN+T N TT + NL+ +Y+++A AFT G GPFS PV MD +Y+ S P + D + +PWF+ + I+ +L+ IF ++ R+ + K+ HL V + ED+ A S ++W+N H W K G ++ + E ND S L EV +D + T S+ PAPYATTTL + ++ G
Sbjct: 122 LRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGL---PVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRR-DGKMPIGRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHSQPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQNTMAMLPCEAEGIPGPNIQWFFKSASV-DVNNPRFSILDSGTLQIDDLQPLDSGLYTCTASSESGETSWSATLSVESPRNPNAIFHRMFDPSTFPGPPGKPTAINVTETAITLRWLRSSKLGDSPLIGYTVEYYSSDLKRGWMVAARRVKRESFVVHNLRPDSHYVFLVRAENSHGLSVPSEISDVICTLGLPSHILPEYDLEEARYYLSTSIVKLKDARAISSTAVKLMWEIQSNLGYVEGFYIRFRDSSGRSQKYNMITVL-NGAATSYVLTDLRKFTKYEFFLVPFYKSVEGPPSNSRKAQTLEDVPSAPPDNLNVKLKNLTVAVISWSSPPPQYRNGILQGYNIHILKNTSQLHSNITTNGTTTLITVYNLTAGHKYSVKAVAFTSVGNGPFSAPVALVMDSSYLNDQHDSGPASTSLTSSIHDTLQQPWFIALIGGILCLLLSIFFIVLFLCRRMAWKKALEAHLPVAAHKSEDVR----AGVSNHEALWIN-------HTWHPTDGKTGQNISESKLLNKETSANDLNYASVYSPLQYEVNGSDYAEVDSHSMTTFYKKGSSSVPAPYATTTLINPSSYKTCTG 996 HSP 2 Score: 157.918 bits (398), Expect = 2.406e-35 Identity = 119/384 (30.99%), Postives = 170/384 (44.27%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSL------VIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD---KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES-SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
E P D + + A L C++ G P P + W NGK++ E R+ L + V+ D G Y C ARN +G S TL V + + F P D+ TL C G P+P + W + +G +V G LVI D+G Y C A N G + S +L+V P F+ +P+D N C+VDG+P P++ W + + + G+ + ++ +L I VR ED G Y+C A + G A L V + P A+ P NQ + +N IA C ASGNP PSI W K NQ ++ S R + + GTL+I + ED Y C+A S T A L V
Sbjct: 29 EHPRDLLVKKQQPAKLNCKA-DGVPTPVIEWYHNGKLV---EGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRRDGKMPI------GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHS-----QPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDV 397 HSP 3 Score: 94.7449 bits (234), Expect = 6.580e-16 Identity = 84/295 (28.47%), Postives = 128/295 (43.39%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE--------NDLALPKGSQVDKGIL-VIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG-STLAKAYLEVTA---IEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKN--QQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
P + P D+ K L C G P P + W N + L G V L V+ D G Y C A N +G+ S++ L V F P+D +V A EC G+P P + W K G + + G++ ++ G LVI+ VR+ D G YVC A ++AG + A L V +P ++A+ +NQ + + C G+P P++ W + + P+ R I+ + +L I +R ED LY C A + G S SA+L+V
Sbjct: 25 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGKLVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNG----LPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLAL--SNQNVDI------ACLVDGDPTPTVTWHRRDGKMPI-----GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTV 302
BLAST of conserved hypothetical protein vs. nr
Match: gi|1101360285|ref|XP_018906445.1| (PREDICTED: roundabout homolog 1 [Bemisia tabaci] >gi|1101360287|ref|XP_018906446.1| PREDICTED: roundabout homolog 1 [Bemisia tabaci]) HSP 1 Score: 785.408 bits (2027), Expect = 0.000e+0 Identity = 441/971 (45.42%), Postives = 604/971 (62.20%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRREN-DLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRN-HQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLIS--SKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKG-NGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHPQDVGDLISEPWFLIFLSSIVIVLMLIFVAIV-MYRRQWSGHKSSLGHLAVPVQRYEDMS----RLHPACSSRDGSMWVNANSGHLHHHWKNGSDLVVQGQKSGECFYNDNSDGQALYAEVGDTDEVLTTFNGFPTSKSDTSNGDPAPYATTTLAMQNRIRSVHG-KTFMALPQHDPSSQDFLSHKTTSSSGGDSLS--NGQKSPGGDFS-------------AGKRTGSSSGHSGGGGNFYLPNWSELF 951
L DDFR EP DTR+A G+TA+L+C P+G P+P++ W K+ + + KR+ L + G+L+I D K+D+G Y C NMVG + S TL V+VKP FI P + T G V C V+G+P P+++W R++ + K +D L I P DEG+Y C A N VG +S S L VH+ PTF P+DQ+VG+NGI FEC+ GNPPPS+FWTKEG+Q LMF G ++G M VS DGTL IQGV+KED GF VCSALSVAGST A+A+L+VT++ED PPPI+ +GP+NQTLP+ ++A LPCQA G P P I W+K+ SSR+ I ++GTL IDNL D+ LYTCTA SESGETSWSASLSVE K P H+TPDPSTFP PP PKI+N +S+T++W + Q G S LIGYT+EYFS +++TGWVVAA RIT+E+ T+N+LKPD+ Y+F+VRAENSHG+S PS++SE VRTL L L+E ++ L KV+ L + +ST++RV W+V+ S ++++EG ++RFRD+SGGSQK+N+ TVM + + Y ++NLRKFT+YE FL PF+K++EGQPSNS+ VQTL D PSAPP V +LNMTSA + + PPPQH NG+L GY++ VKG N S + +TLN++T L+NL+ Y IR A+T+ G GP+S PV MDP P ++ VG+ ++E WFL+ L++++ ++ F A+ + +RQ G + LGHL+VPV D+S +S ++W++ H W+ G D + + + SD YAEV DT + T +N K + G+P PYATTTL N RS + + S LS +S S N Q G D + + +R +S+ S G G++ PNWSE
Sbjct: 133 LKDDFRVEPKDTRVATGETALLECGPPKGQPEPTLIWKKDDQYIDL--GMTKRIQLVDGGNLMISDVKKSDEGKYQCLVENMVGIKESKVATLTVHVKPYFIGEPREATVLAGQSVEFECKVSGEPTPNVLWYRDDAKKTMGKAKTLDDKSLRIESVTPADEGVYVCSAENLVGTISASAPLIVHSPPTFKKSPQDQKVGLNGIVNFECLASGNPPPSVFWTKEGSQLLMFPGNSYGHMLVSSDGTLRIQGVQKEDAGFLVCSALSVAGSTTARAFLQVTSVEDVPPPIVEIGPSNQTLPLQSVATLPCQARGTPPPQIRWYKDGS--SVSASSRVSISSTGTLQIDNLEPGDSGLYTCTASSESGETSWSASLSVE--KTPGPHLHRTPDPSTFPSPPGTPKILNVTKSSITVAWDPSPKQQGASALIGYTLEYFSSDLQTGWVVAAHRITTESITINDLKPDTMYMFIVRAENSHGLSVPSEISEGVRTLGLNTVVPQHL-LDEARSRLGTKVLALKELIPAASTSVRVVWEVVSSTGAEFMEGVYLRFRDLSGGSQKYNLATVMTAEATN-YTVSNLRKFTKYEFFLVPFYKSVEGQPSNSMLVQTLEDVPSAPPDNVQVGMLNMTSAYVRWAAPPPQHHNGMLVGYKIQVKGTNNSKILAQMTLNSSTTSVQLTNLTTGGSYMIRVVAYTRVGQGPYSTPVPLLMDP---HQPPKAY---VGETVNETWFLVLLAALLCTVLSGFFAVFYLKKRQTPGKE--LGHLSVPVVNANDLSLMGGMGMGLMTSGKETLWID-------HGWRGGGDKETETKLLNTGQIKECSD----YAEV-DTRNLSTFYN---PRKEYSQPGNPTPYATTTLLAPNNNRSEECLDSGLMFGASSSSETKTLSSNDSSCKNEQSCRELNAQMRSGNDLNQLYIDEYRQRHSHSSQRKYTSNTASSGSGSYPAPNWSEFL 1072 HSP 2 Score: 188.348 bits (477), Expect = 5.426e-45 Identity = 137/406 (33.74%), Postives = 194/406 (47.78%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSL----VIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLIC-LVAGDPEPHLVWRREN---DLALPKGSQ-VDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESS---RIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASL---SVEDPKNPNIIFHKTPDPSTFP 401
E P D + + + L C++ G P+P++ W K+G+++ + +RV L TGSL V + + D G Y C ARN G S TL V V K F P+D G L C G PEP L+W++++ DL + K Q VD G L+I + DEG Y C N VG K S+ LTVH P F+ +P++ V +FEC V G P P++ W ++ ++ M G+ D +L I+ V D G YVCSA ++ G+ A A L V PP P +Q + +N I C ASGNP PS+ W K L + +++ + GTL I ++ ED C+A S +G T+ A L SVED P I P T P
Sbjct: 40 EHPSDMVVPRNEPVTLNCKA-EGKPEPTIEWYKDGELIKKTPHSNQRV-LLPTGSLFFLRVAHNKKEQDSGVYWCVARNTAGVATSRNATLQVAVLKDDFRVEPKDTRVATGETALLECGPPKGQPEPTLIWKKDDQYIDLGMTKRIQLVDGGNLMISDVKKSDEGKYQCLVENMVGIKESKVATLTVHVKPYFIGEPREATVLAGQSVEFECKVSGEPTPNVLWYRDDAKKTM------GKAKTLDDKSLRIESVTPADEGVYVCSAENLVGTISASAPLIV-----HSPPTFKKSPQDQKVGLNGIVNFECLASGNPPPSVFWTKEGSQLLMFPGNSYGHMLVSSDGTLRIQGVQKEDAGFLVCSALSVAGSTTARAFLQVTSVEDVPPP--IVEIGPSNQTLP 430 HSP 3 Score: 93.5893 bits (231), Expect = 1.405e-15 Identity = 87/310 (28.06%), Postives = 130/310 (41.94%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLA-----------LPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFEC-VVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRME-----DTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTP 395
P + P D+ VTL C G PEP + W ++ +L LP GS + V + D G+Y C A N G S++ L V F V+PKD RV A EC G P P++ W K+ + + G T ++ + G L+I V+K D G Y C ++ G +K V + P P T+ C+ SG P P++ W+++ ++ + M +A TL +LR+E D +Y C+A + G S SA L V P F K+P
Sbjct: 36 PRITEHPSDMVVPRNEPVTLNCKAEGKPEPTIEWYKDGELIKKTPHSNQRVLLPTGSLF--FLRVAHNKKEQDSGVYWCVARNTAGVATSRNATLQVAVLKDDFRVEPKDTRVATGETALLECGPPKGQPEPTLIWKKD--DQYIDLGMTK-RIQLVDGGNLMISDVKKSDEGKYQCLVENMVGIKESK----VATLTVHVKPYFIGEPREATVLAGQSVEFECKVSGEPTPNVLWYRD-------DAKKTMGKAK-TLDDKSLRIESVTPADEGVYVCSAENLVGTISASAPLIVHSPPT----FKKSP 324
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238832924|ref|XP_022253083.1| (roundabout homolog 1-like isoform X5 [Limulus polyphemus]) HSP 1 Score: 783.097 bits (2021), Expect = 0.000e+0 Identity = 408/816 (50.00%), Postives = 537/816 (65.81%), Query Frame = 0
Query: 7 LGDDFREEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNVKPIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRENDLALPKG-SQVDKGI-LVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAGSDDDLNLEEVQNHLLDKVVELMSIEAISSTTIRVSWKVLISSKYVEGFFVRFRDMSGGSQKFNMKTVMKQDNIDSYVITNLRKFTEYEVFLTPFFKNIEGQPSNSLHVQTLADTPSAPPTTVMAEILNMTSAQITLSPPPPQHRNGILQGYQVHVKGNGSTFHSNVTLNATTMHFILSNLSLNEQYTIRASAFTQAGLGPFSRPVTFTMDPAYIKSPVVSHP------QDVGDLISEPWFLIFLSSIVIVLMLIFVAIVMYRRQWSGHKSSLGHLAVPVQRYEDMSRLHPACSSRDGSMWVNANSGHLHHHWKNGSDLV 814
L DDFR P D ++A+ D+A L+C PRG+P P VSW KNG ++ E RV +T +G+LVI D + DQG YVC A NM G RNS P L V VKP FI+ PEDV + V + CLV GDP P + W R D +P G +Q+ K L I + DEG+Y CEA N VG S S LTVH+ P+F VKPK+QRVG+NGIAKFECV GNPPPSIFWTKEGNQ LMF ++G+ V+ GTL+I GV KED G+YVCSALSV ST+ KA+L+V A+ D PPPII +GPANQTLP NT+A+LPC+A G P P+I WF + V + R I SGTL ID+L+ D+ LYTCTA SESGETSWSA+LSVE P+NPN IFH+ DPSTFP PP KP +N T++T+ W R+ + G SPLIGYTVEY+S +++ GW+VAA R+ E++ V+NL+PDS YVFLVRAENSHG+S PS++S+ + TL + + +LEE + +L +V+L AISST +++ W++ + YVEGF++RFRD SG SQK+NM TV+ SYV+T+LRKFT+YE FL PF+K++EG PSNS QTL D PSAPP + ++ N+T A I+ S PPPQ+RNGILQGY +H+ N S HSN+T N TT + NL+ +Y+++A AFT G GPFS PV MD +Y+ S P + D + +PWF+ + I+ +L+ IF ++ R+ + K+ HL V + ED+S P +GS + +S + +K GS V
Sbjct: 129 LRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGL---PVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRR-DGKMPIGRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHSQPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQNTMAMLPCEAEGIPGPNIQWFFKSASV-DVNNPRFSILDSGTLQIDDLQPLDSGLYTCTASSESGETSWSATLSVESPRNPNAIFHRMFDPSTFPGPPGKPTAINVTETAITLRWLRSSKLGDSPLIGYTVEYYSSDLKRGWMVAARRVKRESFVVHNLRPDSHYVFLVRAENSHGLSVPSEISDVICTLGLPSHILPEYDLEEARYYLSTSIVKLKDARAISSTAVKLMWEIQSNLGYVEGFYIRFRDSSGRSQKYNMITVL-NGAATSYVLTDLRKFTKYEFFLVPFYKSVEGPPSNSRKAQTLEDVPSAPPDNLNVKLKNLTVAVISWSSPPPQYRNGILQGYNIHILKNTSQLHSNITTNGTTTLITVYNLTAGHKYSVKAVAFTSVGNGPFSAPVALVMDSSYLNDQHDSGPASTSLTSSIHDTLQQPWFIALIGGILCLLLSIFFIVLFLCRRMAWKKALEAHLPVAAHKSEDVSVYSPLQYEVNGSDYAEVDSHSMTTFYKKGSSSV 938 HSP 2 Score: 157.532 bits (397), Expect = 2.849e-35 Identity = 125/406 (30.79%), Postives = 177/406 (43.60%), Query Frame = 0
Query: 13 EEPDDTRIAQGDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSL------VIRDAVKADQGDYVCQARNMVGTRNSGPGTLHVNV-KPIFIQRPEDVTSKGGSEVTLICL-VAGDPEPHLVWRRENDLALPKGSQVD---KGILVIPKAEPHDEGMYSCEATNPVG-KVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFK--NQQPLYAVES-SRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKN-PNIIFHKTPDPSTFPQ 402
E P D + + A L C++ G P P + W NGK++ E R+ L + V+ D G Y C ARN +G S TL V + + F P D+ TL C G P+P + W + +G +V G LVI D+G Y C A N G + S +L+V P F+ +P+D N C+VDG+P P++ W + + + G+ + ++ +L I VR ED G Y+C A + G A L V + P A+ P NQ + +N IA C ASGNP PSI W K NQ ++ S R + + GTL+I + ED Y C+A S T A L V + P I P T PQ
Sbjct: 36 EHPRDLLVKKQQPAKLNCKA-DGVPTPVIEWYHNGKLV---EGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLALSNQNVDIACLVDGDPTPTVTWHRRDGKMPI------GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTVHS-----QPSFAVKPKNQRVGLNGIAKFECVASGNPPPSIFWTKEGNQALMFPENSYGRFSVTSLGTLIISGVIKEDKGYYVCSALSVVASTMTKAHLDVIAVADLPPPIIRLGPANQTLPQ 426 HSP 3 Score: 94.3597 bits (233), Expect = 7.790e-16 Identity = 84/295 (28.47%), Postives = 128/295 (43.39%), Query Frame = 0
Query: 105 PIFIQRPEDVTSKGGSEVTLICLVAGDPEPHLVWRRE--------NDLALPKGSQVDKGIL-VIPKAEPHDEGMYSCEATNPVGKV-SQSVVLTVHA-HPTFLVKPKDQRVGMNGIAKFECV-VDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALSVAG-STLAKAYLEVTA---IEDQPPPIIAMGPANQTLPVNTIAVLPCQASGNPKPSINWFKN--QQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESGETSWSASLSV 381
P + P D+ K L C G P P + W N + L G V L V+ D G Y C A N +G+ S++ L V F P+D +V A EC G+P P + W K G + + G++ ++ G LVI+ VR+ D G YVC A ++AG + A L V +P ++A+ +NQ + + C G+P P++ W + + P+ R I+ + +L I +R ED LY C A + G S SA+L+V
Sbjct: 32 PRITEHPRDLLVKKQQPAKLNCKADGVPTPVIEWYHNGKLVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDDFRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNG----LPVDSEGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVRVKPYFIKEPEDVLAL--SNQNVDI------ACLVDGDPTPTVTWHRRDGKMPI-----GRAQIQKNKSLRITQVRTEDEGLYMCEAENAVGIASGSATLTV 309 HSP 4 Score: 77.7962 bits (190), Expect = 9.443e-11 Identity = 67/213 (31.46%), Postives = 93/213 (43.66%), Query Frame = 0
Query: 178 VGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNPPPSIFWTKEGNQELMFSGTTHGQMHVSQDGTL-----VIQGVRKEDGGFYVCSALSVAGSTLAK-AYLEVTAIEDQPPPIIAMGPANQTLPVNTIAVLPCQ-ASGNPKPSINWFKNQQPLYAVESSRIMIEASGTLVIDNLRMEDTALYTCTAGSESG-ETSWSASLSVE 382
+ SQS + HP L+ K Q AK C DG P P I W G + GT++ M V+ G V+ G R +D G Y C A + G ++ A LEV + D P + + A L C G+P P ++W KN P+ + E R+ I SG LVI+++R D Y C A + +G S A LSV
Sbjct: 23 LASASQSRAPRITEHPRDLLVKKQQP------AKLNCKADGVPTPVIEWYHNGK---LVEGTSNRIMLVNGVGVALFFLHVLHGKRDQDTGTYWCVARNHLGEARSRNATLEVAILRDD----FRANPRDIQVAREDSATLECAPPRGHPDPKVSWEKNGLPVDS-EGGRVRITGSGNLVIEDVRQTDQGKYVCIAENMAGVRNSSPAILSVR 221 The following BLAST results are available for this feature:
BLAST of conserved hypothetical protein vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of conserved hypothetical protein vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of conserved hypothetical protein vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold108_size357748:284957..288989- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold108_size357748-snap-gene-2.20 ID=maker-scaffold108_size357748-snap-gene-2.20|Name=conserved hypothetical protein|organism=Tigriopus kingsejongensis|type=gene|length=4033bp|location=Sequence derived from alignment at scaffold108_size357748:284957..288989- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'conserved hypothetical protein' has the following synonyms
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