BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 766.148 bits (1977), Expect = 0.000e+0
Identity = 364/607 (59.97%), Postives = 465/607 (76.61%), Query Frame = 0

Query:   12 MRNLTTP----ANMSRRGSSSSVGPPPLASSHQGRYKGLLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKA-GGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPLSTEEISKQTEPEWKLAP 597
            MRNL TP    A+ SRRGSS  +    L+S +Q RYKGL+ST G+VKFSAWKRE +I +   HEF+L KI EK+P+++W+KD+++GR E +G V S V+K+ASGLTK GF K DV+ + CSN+VEYW++AL  W CGG VMPVNCE+  D LE Q+ E+K K++VCDDFNY D LD  ENT +++ +I+IG E  A    I M DL+ DDGK  P ++   W +DV ++PFTS SSG + GI+HT+KSLM+ F+SPDGAANH+ DQ+IGDSI C N+FFHM+GFY  AL SIYG+S+Y+LSE+S+  F+E +VD KT + + Y WQIR+LSQS  +  YDLS LK +++  G+LSSTIRME+L+R P++KYVREAYGLNECGLVTLTYP+EKKNSIA++K  + P+DH+IP+GLPNMY+ +KII+RQ    V+GPD+QGEIC+KS Q  +GYLN++    FD++ +FHTGDLGYYD+QG++Y++EKI NLI FWMYEVAP++LESRLLGSNNIVDAAVVGIP+KENG                EENLTNLMESRLQDHERIRGGLYF+QNIPRDENWKVLR VL  Y+P     I +  +   +L P
Sbjct:    1 MRNLATPSFSGASNSRRGSSVGL-VSMLSSPNQARYKGLISTAGIVKFSAWKREXEIQKLNFHEFILQKIGEKEPDRIWIKDLSIGRMERFGNVHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQDKVILMTDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSG-AKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSEQNFLEALVDXKTASISCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKEESSAFDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVLAEYKPHVNNLIKRPNKLFKQLHP 605          

HSP 2 Score: 64.3142 bits (155), Expect = 7.824e-11
Identity = 31/94 (32.98%), Postives = 54/94 (57.45%), Query Frame = 0

Query:  748 LSYWLKVYINPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNV 841
            + +W+   + P++LE  ++    + D +V        G +PRAFVVLK G+E T E + +   +RL D + +RGG+ F   +P+D N K++ +V
Sbjct:  489 IQFWM-YEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSV 581          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 155.992 bits (393), Expect = 3.598e-40
Identity = 134/568 (23.59%), Postives = 250/568 (44.01%), Query Frame = 0

Query:   36 ASSHQGRYKGLLSTPG---VVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDL-IEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGD------SIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFD---SEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG---------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQ 575
            A      +   L  PG   ++++S ++ E  +P     +F+   +++    +  +  +T GRT TY  V S   K  S L +LG  K DV+ +   N  E+ +  +     G  +  +N     + +  Q+  + AK ++      +++    E    ++ +IVIG E     C    ++ I DDG     R  +   +D+  LP++S ++G   G+  TH +L+         + H     IGD      SIA   +F   +   +++    +GV + +L ++    F+  +V  K     + P  +  ++ S  I    LS +K +         T+  + + +  +    +E YG+ EC  VT   PK+         ++     ++IP       T  KII+  + + V GP E GE+C+   Q   GY    NK+  D     G+ HTGD+ YY+  G  Y +++++ LI     +VAP+ LE  +     + D A VG+PD+ NG               E+ + + +  ++  H+++  G+ F+  +P+++  K+LR  L+
Sbjct:   27 AKQSXSHFGAPLIKPGEEKLIQYSPYE-EVVLPSIPYADFIWENLEDYKEREALVCGMT-GRTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIH---NFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDG--------IIYGSCGNLIP------NTIGKIIDIDTGK-VLGPGENGELCVSGPQIMQGYYK--NKKATDKIIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELK 572          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000002083 (supercontig:LSalAtl2s:LSalAtl2s138:399496:447452:1 gene:EMLSAG00000002083 transcript:EMLSAT00000002083 description:"maker-LSalAtl2s138-snap-gene-4.7")

HSP 1 Score: 118.627 bits (296), Expect = 1.239e-30
Identity = 68/189 (35.98%), Postives = 103/189 (54.50%), Query Frame = 0

Query:  668 RNRSRRGSMDCILEDKPSALGATGGRKGSQAATSGKRRSSETRRVSAGGTRGGGGSRRGSGSEIPIVPLTGSASSAVEGGLSYWLKVYINPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNVEKLSQEAHVVDTHFI 856
            R + RR SMD I             R+G  +   G     ++RR+S+       GSRRGS   +  +    S+    +  L+YWL++ IN   LE  + SHPAV+D  V+  T+ G+G LPR +V LK GY+ ++E+IL   +A++ DT+ LRGG+V  DK+ +D NGK++ N+EK  +     D   I
Sbjct:   27 REKDRRSSMDRI-------------REGEVSVNVGSSAPGKSRRMSSAR-----GSRRGS--XVDALSSLESSHDMGQNNLTYWLQITINTEALEEYLKSHPAVEDAVVRGVTIEGIGELPRGYVSLKQGYDTSSEDILSNMNAKVLDTEQLRGGLVIIDKIGRDMNGKVIMNLEKYDKNVVGTDNDLI 195          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 71.2478 bits (173), Expect = 6.283e-13
Identity = 111/501 (22.16%), Postives = 204/501 (40.72%), Query Frame = 0

Query:  105 VDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWD-EDVAFLPFTSASSGCSAGIMHTHKSLM-AWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAI----------IKY--DLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQN-FIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSN-NIVDAAVV-----------GIPDKENG----EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRD 572
            V K A  L + G  K DV+ +   N  + ++   A   C G V      I  + L  +V E +AK ++C +  Y  V D++         I+I  + +    +     I+   +T  P   + +D + +A + +TS ++G   GI     SL    F SP    N          I C   F H+SGF      SI    V +    S      L V+    T +  P+   ++S S  +          I+Y  +   +K++  +G      I  +L + F  ++ V   Y + E G + L    ++ N      +                  +V I +  S E + GP+  GE+  ++     +GYLN  ++ K+ F  +G+  +GD+ +Y+  G ++Y  +I+ ++      V P  +E+ +   N NI++A +            GI   + G     E +   +  R+ D++ +   +   + +PR+ N K+LR+
Sbjct:   83 VPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTYYKVKDLSS--------IIINLDTQLHAILE----IDMSTQTQIPE-QIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLLFLSPKTDGNML--------ITCN--FAHVSGFNYSCFWSI-NFDVCTFHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNM------------------EVYIRDIHSGERL-GPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKIQTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRN 540          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 70.0922 bits (170), Expect = 1.149e-12
Identity = 79/322 (24.53%), Postives = 141/322 (43.79%), Query Frame = 0

Query:  284 FFHMSGFYTVALASIY-----GVSVYSLSEYSDNAFVELIVDNKTQTANLY----PWQIRLLSQSKAII-KYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIV-DAAVVGIPDKENGEENLT---------------NLMESRLQDHER-----IRGGLYFIQNIPRDENWKVLRDVL 574
            FFH  GF T  L +I      G+ + S +EY D +   L    +T   N+      + +++   S  +  ++DLS +  +   GG +++    +   +FP++K + + YG  E   ++L    +    +                  P    Q++I+N   N    GP+  G+I +K       Y+  D++     +GFF+ GD+GYYDS G I++  + ++L+    + V P  +ES +    + V D  V G  DK NG E L+               N++E   + ++      ++GG+     IPR+ N K+ R  L
Sbjct:  252 FFHPGGF-TEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQHLGRL-----------------FPKY--QLRILN--PNGEPLGPNSVGDIVVKPGYGMRRYIG-DHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDK-NGIERLSGVIVLHHIEKGLSLQNVLERTFEAYKFPRYKWLQGGVRVTSAIPRNANGKIFRGRL 549          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 68.1662 bits (165), Expect = 5.595e-12
Identity = 101/465 (21.72%), Postives = 198/465 (42.58%), Query Frame = 0

Query:  155 EFDLLEEQVTEAKAKVIVCDDFNY--------EDVLDMA--ENTELLKHVIVIGTEGK------AGGCIPMND-LIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFF-HMSGFYTVALASIYGVSVYSLSEYSD-NAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGL-PNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGY-LNEDNKRLFDS-----EGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGEENLTNLMESRLQD-------------HERIRG-----GLYFIQNIPRDENWKVLRDVLQ 575
            E D L E+    KA VI+  D  Y        ++V+D A  ++++ +KHV+V    GK      +G  + + D +++ + +  P  ++    ED  F  +TS S+G   G++H+    + +          + +  I   +A   W   H    Y   +     V   S   Y D + + +L+ +         P  IR L + +    Y L+  KS + + G +   I +E         +  +  G N C +V   +  E      T +  E   + V  +G+ P +     +++ + NE ++  ++QG +C+K+    +   + +D+KR  ++      G++ TGD  Y   +G I  + +I+++I+   + +    +E  L   +NIV+AAVVG P    GE     ++   L +              + I G        F +++P+  + K++R +L+
Sbjct:  112 EKDELGEREFNCKANVIITVDEGYRGGRVIPLKEVVDEAIKKSSQPIKHVLVAKRSGKGHVKMKSGRDVYLEDAMLQVESECHPESMS---SEDPLFYLYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVLFESTPTYPDPSRYWKLVEELGITQFYTSPTAIRQLMKYED--SYVLNSDKSSLRTLGSVGEPINIEAW------NWYNDLVGRNRCDIVDTWWQTETGGICITPRPSELNAEIVPALGMRPFLGINPVLLDDKGNE-IKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLVKDNISGFAVPEKFQFCEDLPKTRSGKIMRRILR 564          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 61.2326 bits (147), Expect = 6.494e-10
Identity = 43/156 (27.56%), Postives = 77/156 (49.36%), Query Frame = 0

Query:  440 GPDEQGEICIKSAQ-NFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLG-SNNIVDAAVVGI---------------PDKENGEENLTNLMESRLQDHERIRGGLYFIQN-IPRDENWKVLRDVLQ 575
            GP++ GE+  + A     GYLN  E+N+++F+ +G+ HTGD  +Y  QG  ++  +I  +I      V P  LE  L    ++I++  VV +                DK+   E +   +  ++ +++RI G L   +  +PR+   K+LR  +Q
Sbjct:  329 GPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEKKQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQ 484          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 53.1434 bits (126), Expect = 2.800e-7
Identity = 61/259 (23.55%), Postives = 115/259 (44.40%), Query Frame = 0

Query:  287 MSGFYTVALASIYGVSV-YSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYD-------LSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGEE 535
            +S F  +  A + G  + + L  +  N +V L       T  L    I L   SK ++  D       L+C+ +   +    + T+ + +LE     K +R  YG  E G V+          IA  K  ++    +  VG P  + + KI+++  N  +   +  GE+ +++     GY  +   +K++   + ++H+GDLG    +G    I ++++++      + P  +E  LL   +IV+  +VG+PD   GEE
Sbjct:   19 LSHFCVINNALLTGNRIGFDLKYHXINLWVPLFHTFGCVTGTL----ISLCYGSKLVLPSDGFDPTACLNCIPAEKCTYLFGTPTMYIAVLE---AQKKLRNIYGATETGPVSF---------IACPK--DSFEVRINTVGPPLGHIEAKIVDQ--NGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEE 257          

HSP 2 Score: 51.9878 bits (123), Expect = 7.375e-7
Identity = 49/211 (23.22%), Postives = 98/211 (46.45%), Query Frame = 0

Query:  329 YPWQIRLLSQ--SKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGEE 535
            YP  ++   +  S  + K+++  +K  I+ GG        +  E+   +K +   YG  E G V+          IA  K  ++    +  VG P  + + KI+++  N  +   +  GE+ +++     GY  +   +K++   + ++H+GDLG    +G    I ++++++      + P  +E  LL   +IV+  +VG+PD   GEE
Sbjct:  289 YPMFVKDFPKTLSGKVKKFEMKAIKLGIT-GGSNCLVEACKDFEKHLNMKSLMNIYGATETGPVSF---------IACPK--DSFEVRINTVGPPLGHIEAKIVDQ--NGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEE 485          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75296548|sp|Q7XXL2.2|4CLL9_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 9)

HSP 1 Score: 187.963 bits (476), Expect = 1.071e-49
Identity = 141/539 (26.16%), Postives = 259/539 (48.05%), Query Frame = 0

Query:   69 IHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP--PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLS--CLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPK-------EKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDK----------------ENGEENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            + EF+L   +        ++    GR+ TYG+VA +  + A  L  +G  K  V+ +   N   Y +V+L     G     VN       +++QV +++AK++V ++  ++ V D          VI +G   +  G I  + L+    +T      ++ +   D+  LP++S ++G S G+M +H++L++   S   A      +  G  +  G   FFH+ G   +  A++ +  +V  +  +    F+  +VD++   A L P  +  + +S    ++DLS   LKSV+++   L+  +      +FP V+ V EAYGL E   +TLT+          KK+S+                 LPN+  +VK ++  +  ++   +  GE+C++S     GY    E+ +R  D +G+ HTGD+GY D  G ++ +++I+ LI +  ++VAP  LE+ LL   ++ DAAV G+PD+                E GEE +   +  R+  ++R+R  L+ +  IP+  + K+LR  L++
Sbjct:   35 VPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTARFARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSEAKLVVANEVAFDKVKDAGVP------VIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSDLCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAV---APETAGQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLALKSVMTAAAPLAPDLLAAFQRKFPGVQ-VEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGFI--------------LPNL--EVKFVDPDTGRSLPA-NTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEEEIVAYVAERVASYKRVR-VLHIVDAIPKSVSGKILRRQLRD 545          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75311450|sp|Q9LQ12.1|4CLL1_ARATH (RecName: Full=4-coumarate--CoA ligase-like 1; AltName: Full=4-coumarate--CoA ligase isoform 10; Short=At4CL10)

HSP 1 Score: 185.652 bits (470), Expect = 5.702e-49
Identity = 154/529 (29.11%), Postives = 265/529 (50.09%), Query Frame = 0

Query:   71 EFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASIYGV-SVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLK--SVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTY-PKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYL--NEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            EF+L  ++E   N  +++ VT G+  TYG V  +  ++A  LT LG  K  V+ +   N  EY ++AL     GG     N       +++QV  + A+ I+ D  NYE V  +         VIV+G E K  G +   DL+E   K            D+  LPF+S ++G   G+M TH++L+A   S          +MIG  +  G   FFH+ G   +  A++     V ++S Y    F+  ++ ++   A + P  I  L ++  + ++DLS LK  SV+++   L+  +      +FP V+ V+EAYGL E   +TLT+   EK   IA          + +   LPN+  +VK I+  +  ++   +  GE+C++S     GY    E+  +  D +G+ HTGD+GY D  G I+ +++I+ LI +  ++VAP  LE+ LL   ++ D AVV +PD+E G                EE++ N + + +  ++++R  ++F+ +IP+  + K++R +L++
Sbjct:   30 EFVLQGVEEYTENVAFVEAVT-GKAVTYGDVVRDTKRLAKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGIITDATNYEKVKSLG------LPVIVLGEE-KIEGAVNWKDLLEAGDKCGDTDNEEILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVR---SEMIGQIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSVMTAAAPLAPELLTAFEAKFPNVQ-VQEAYGLTEHSCITLTHGDPEKGQGIA--------KRNSVGFILPNL--EVKFIDPDTGRSLP-KNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVINPKATEKEEDILNFVAANVAHYKKVR-AVHFVDSIPKSLSGKIMRRLLRD 534          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|41688574|sp|Q27757.2|LUCI_PHOPE (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 182.185 bits (461), Expect = 8.409e-48
Identity = 139/504 (27.58%), Postives = 237/504 (47.02%), Query Frame = 0

Query:  107 KIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCE-IEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIE-------DDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIP--VGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            ++A    K G  + D I +C  N ++++L  +A+   G    PV+ + IE +L+   +   K ++I C    ++ VL++    + ++ +I++      GG   +N+ I        D  K  P   N   D+ VA + F+S ++G S G+M THK+++A F       +H  D   G++I           F H  G  T       G  V  +  + +  F++ + D K ++  L P  +    +S  + KYDLS LK + S G  LS  I   + +RF  + +VR+ YGL E                 T+ VL TP+  V P   G    +  VK+++  + + + GP+E GE+  K       Y N  E  K + + +G+  +GD+ YYD+ G  Y ++++++LI +  Y+VAP  +E  LL    IVDA V GIPD+  G                E+ + N + S++   + +RGG+ F+  IP+    K+ R VL+ 
Sbjct:   61 RLAESFKKYGLKQNDTIAVCSENGLQFFLPLIASLYLGIIAAPVSDKYIERELIH-SLGIVKPRIIFCSKNTFQKVLNVKSKLKYVETIIILDLNEDLGGYQCLNNFISQNSDINLDVKKFKPNSFN--RDDQVALVMFSSGTTGVSKGVMLTHKNIVARF-------SHCKDPTFGNAINPTTAILTVIPFHHGFGMTTTLGYFTCGFRVALMHTFEEKLFLQSLQDYKVESTLLVPTLMAFFPKSALVEKYDLSHLKEIASGGAPLSKEIGEMVKKRF-KLNFVRQGYGLTE----------------TTSAVLITPDTDVRPGSTGKIVPFHAVKVVDPTTGK-ILGPNETGELYFKGDMIMKSYYNNEEATKAIINKDGWLRSGDIAYYDNDGHFYIVDRLKSLIKYKGYQVAPAEIEGILLQHPYIVDAGVTGIPDEAAGELPAAGVVVQTGKYLNEQIVQNFVSSQVSTAKWLRGGVKFLDEIPKGSTGKIDRKVLRQ 536          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|74897041|sp|Q54P77.1|4CL1_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 178.718 bits (452), Expect = 1.228e-46
Identity = 146/573 (25.48%), Postives = 253/573 (44.15%), Query Frame = 0

Query:   46 LLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVL-DMAENTELLKHVIVIGTEGKA------------GGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-------GDSIACGNWFFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEP 579
            ++   G   F++      IP + + + +L  I+ K P++V L D    +  +   VA  ++K+A GL KL   K DV+ +   N  EY  +       GG    VN +   + L   +     + +      YE +  D+      ++ VI++   G+             G  +  N LI ++GK  P  + +   +D A +PF+S ++G   G+  +H ++++         N Y  Q I        D++     FFH+ G     +  +  G  V  L ++    F+ELI   K   + + P    + ++S  + K+DLS L+++ S    LSS +   + ERF     +++ YG  E        P     S  +A +L           LPN     KII+ ++ EN+ G  E+GEICIK     +GY N +     + D +GF  TGD+GY D  G  + +++ + LI    ++V P  LE+ LL    + DA VVG+   + G                E+ L +    ++ +++  RGG++FI  IP+    K+LR  L+++ P
Sbjct:    2 IIKVKGQTYFTSKYPNIIIPEKPVPQLILKHIRSK-PDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLINNNGKDYP-IVRIDPKKDTAIIPFSSGTTGLFKGVCLSHHNIVS---------NTYQTQTIETSTYKKNDTVMGILPFFHIYGLMLFLMLMVKQGHRVVVLPKFEPVRFLELIQKYKVAISFIVPPVAIMFAKSPIVDKFDLSSLRTLFSGAAPLSSEVEDLIKERFKGRLVIKQGYGATELSPACFVIPSGLVKS-GSAGIL-----------LPNQLA--KIISPETGENL-GMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDFNP 548          

HSP 2 Score: 72.0182 bits (175), Expect = 9.549e-12
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP V D  V   +   +G +PR FVV+K     T +E+L +AH ++A+  H RGGI F   +PK   GKL+
Sbjct:  457 VPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLL 539          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|74897042|sp|Q54P78.1|4CL2_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 177.948 bits (450), Expect = 2.556e-46
Identity = 147/568 (25.88%), Postives = 252/568 (44.37%), Query Frame = 0

Query:   51 GVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVL-DMAENTELLKHVIVIGTEGKA------------GGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-------GDSIACGNWFFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEP 579
            G + F++      IP + I   +L  I+ K P++V L D    +  +   VA  ++K+A GL KL   K DV+ +   N  EY  +       GG    VN +   + L   +     + +      YE +  D+      ++ VI++   G+             G  +  N LI ++GK  P  + +   +D A +PF+S ++G   G+  +H +L++         N +  Q +        D++     FFH+ G  T  +  +  G  V  L ++    F++LI   K   + + P    + ++S  + K+DLS L+++ S    LS  +   + ERF     +++ YG  E        P     S  +A +L           LPN    VKII+ ++ EN+ G  E+GEICIK     +GY N +     + D +GFF TGD+GY D  G  + +++ + LI    ++V P  LE+ LL    + DA VVG+   + G                E+ L +    ++ +++  RGG++FI  IP+    K+LR  L++  P
Sbjct:    7 GQIYFTSKYPNIIIPEKPIPHLILKHIRSK-PDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIGQLTLSSDGIVMSFNQLINNNGKDYP-IVRIDLKKDTAIIPFSSGTTGLFKGVCLSHHNLVS---------NTHQTQTVETTNYKKNDTVMGQLPFFHIYGLMTYLILMVKQGHCVVILPKFEFVRFLDLIQKYKVAISFIVPPIAIMFAKSPIVDKFDLSSLRTLFSGAAPLSREVEDLIKERFKGKLIIKQGYGATELSPACFVIPSGLIKS-GSAGIL-----------LPNQL--VKIISPETGENL-GMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFFKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDINP 548          

HSP 2 Score: 72.0182 bits (175), Expect = 1.106e-11
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP V D  V   +   +G +PR FVV+K     T +E+L +AH ++A+  H RGGI F   +PK   GKL+
Sbjct:  457 VPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLL 539          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|12229666|sp|Q9S777.1|4CL3_ARATH (RecName: Full=4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumarate--CoA ligase isoform 3; Short=At4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 175.252 bits (443), Expect = 2.621e-45
Identity = 144/544 (26.47%), Postives = 251/544 (46.14%), Query Frame = 0

Query:   55 FSAWKRETDIPRQY-IHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLV---TLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPD------------KENG----EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQ 575
            F +   + DIP    +H +   K+     +K  L   + G++ TYG+      ++ASGL KLG  K DVI +   N  E+    + A   G      N       L +Q+  + AK+I+      + + ++ EN  L     +   E     C+P + LI DD   P         +D A LPF+S ++G   G++ THKSL+          N  +     D I C    FH+    +V L S+  G +V  + ++   A ++LI  ++   A L P  +  L+++  +  YDLS ++ V+S    L   ++  L  R P    + + YG+ E G V   +L + KE   + + +             G      ++K+++ ++  ++ G ++ GEICI+  Q    YLN  E      D EG+ HTGD+GY D    I+ +++++ +I F  ++V P  LES L+  ++I DAAVV   D            + NG    EE++   +  ++  ++R+   ++F+ +IP+  + K+LR  L+
Sbjct:   35 FRSKLPDIDIPNHLPLHTYCFEKLSSVS-DKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTL-----ITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQA-ILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGS------------CGTVVRNAELKVVHLETRLSL-GYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLH-KVFFVASIPKSPSGKILRKDLK 557          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|61213879|sp|Q26304.1|LUCI_LUCMI (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 174.096 bits (440), Expect = 4.991e-45
Identity = 129/503 (25.65%), Postives = 232/503 (46.12%), Query Frame = 0

Query:  107 KIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDD---GKTPPPRLNL---SWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPR----DENWKVLRDVLQ 575
            ++A  +   G    + I +C  N  E+++  LA    G  V P N       L   +  A+  ++         VL++ +    +K ++++ ++   GG   M   I+     G  P   + +   +  + VA L  +S S+G   G+  TH+  +  F       +H  D + G+ ++ G        F H  G +T       G  V  L+++ +  F+  + D K  +  L P    +L++S+ I K+DLS L  + S G  L+  +   +  RF  +  VR+ YGL E     +  P+      A+ KV        +P+       +VK+I+  + + + G + +GEIC+K     +GY N  E  +   D EG+ HTGD+GYYD     + ++++++LI +  Y+V P  LES LL   NI DA V G+PD + G                E+ + + + S++ +H+R+RGG+ F+  +P+      + KV+R++L+
Sbjct:   63 RLAEAMKNFGMKPEEHIALCSENCEEFFIPVLAGLYIGVAVAPTNEIYTLRELNHSLGIAQPTIVFSSRKGLPKVLEVQKTVTCIKKIVILDSKVNFGGHDCMETFIKKHVELGFQPSSFVPIDVKNRKQHVALLMNSSGSTGLPKGVRITHEGAVTRF-------SHAKDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYFACGYRVVMLTKFDEELFLRTLQDYKCTSVILVPTLFAILNKSELIDKFDLSNLTEIASGGAPLAKEVGEAVARRF-NLPGVRQGYGLTETTSAFIITPEGDDKPGASGKV--------VPL------FKVKVIDLDTKKTL-GVNRRGEICVKGPSLMLGYSNNPEATRETIDEEGWLHTGDIGYYDEDEHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKIDAKVIREILK 542          

HSP 2 Score: 60.077 bits (144), Expect = 4.456e-8
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNV 841
            + P  LE V++ HP + D  V     P  G LP A VV++ G   T +EI+ + ++++ +   LRGG+ F D++PK   GK+   V
Sbjct:  451 VPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKIDAKV 536          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|182627620|sp|Q54P79.2|4CL3_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 172.555 bits (436), Expect = 1.711e-44
Identity = 148/570 (25.96%), Postives = 250/570 (43.86%), Query Frame = 0

Query:   51 GVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVL-DMAENTELLKHVIVIGTEGKA------------GGCIPMNDLIEDDGKT-PPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-------GDSIACGNWFFHMSGFYT-VALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPL 580
            G   F++      IP + I   +L  I+ K P++V L D    +  +   VA  ++K+A GL KL   K DV+ +   N  EY  +       GG    VN +   + L   +     + +      YE +  D+      ++ VI++   G+             G  +  N L  ++GK  P  R++L+  +D A +PF+S ++G   G+  +H +L++         N Y  Q I        DS+     FFH  G    + L    G  + +L ++    F+ELI   K   + + P    + ++S  + K+DLS L+++      L S I   + ERF     +++ YG  E        P     S ++  +L            PN+    KII+ ++ EN+ G  E+GEICIK     +GY N +     + D +GF  TGD+GY D  G  + I++ + LI    ++V P  LE+ LL    + DA VVG+   + G                E+ L +    ++ +++  RGG++FI  IP+    K+LR  L+++  L
Sbjct:    7 GQTYFTSKYPNIIIPEKPIPHLILKHIRYK-PDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLTNNNGKDYPIVRIDLT--KDTAIIPFSSGTTGLFKGVCLSHYNLVS---------NTYQTQTIETSTYKKNDSVIGVLPFFHSFGLMLHIMLMVKQGYRIVTLPKFEPVRFLELIKKYKVAMSFIVPPIAIMFAKSPIVDKFDLSSLRTLFCGAAPLGSEIEDLIKERFKGRLVIKQGYGATELSPCCFVTPNGLVKSGSSGTLL------------PNLLA--KIISSETGENL-GMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYFFIIDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDFNNL 549          

HSP 2 Score: 72.0182 bits (175), Expect = 9.972e-12
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP V D  V   +   +G +PR FVV+K     T +E+L +AH ++A+  H RGGI F   +PK   GKL+
Sbjct:  457 VPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLL 539          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 172.555 bits (436), Expect = 1.776e-44
Identity = 127/488 (26.02%), Postives = 230/488 (47.13%), Query Frame = 0

Query:  123 ICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP-------PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------ENLTNLMESRLQDH--------ERIRGGLYFIQNIPRDENWKV----LRDVLQ 575
            I +C  N  E+++  +A    G  V P N       L   +  +K  ++       + V+ + +    +K ++++ ++    G   ++  I+ +  TPP         + +   E VA +  +S S+G   G+  TH++ +  F       +H  D + G+ ++ G        F H  G +T     I G  V  L+++ +  F++ + D K  +  L P    +L++S+ + KYDLS L  + S G  LS  +   +  RF  +  VR+ YGL E     +  P+      A+ KV        +P+       + K+I+  + +++ GP+ +GE+C+K      GY+N  E  K L D EG+ HTGD+GYYD +   + ++++++LI +  Y+V P  LES LL   +I DA V G+PD   GE        E+  N+ E  + D+        +R+RGG+ F+  +P+    K+    +R++L+
Sbjct:   80 IALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRN--TPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRF-------SHARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTSVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKV--------VPL------FKAKVIDLDTKKSL-GPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREILK 542          

HSP 2 Score: 63.5438 bits (153), Expect = 3.745e-9
Identity = 30/82 (36.59%), Postives = 49/82 (59.76%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKL 837
            + P  LE V++ HP++ D  V     P  G LP A VVL+SG   T +E++ +  +++++   LRGG+ F D++PK   GK+
Sbjct:  451 VPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 168.703 bits (426), Expect = 3.750e-43
Identity = 122/488 (25.00%), Postives = 229/488 (46.93%), Query Frame = 0

Query:  123 ICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP-------PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPD----------------KENGEENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKV----LRDVLQ 575
            I +C  N  E+++  LA    G  V P N       L   +  +K  ++       + V+ + +    +K ++++ ++    G   M++ I+ +  TP          + ++  E VA +  +S S+G   G+  TH++ +  F       +H  D + G+ ++ G        F H  G +T       G  +  L+++ +  F++ + D K  +  L P    +L++S+ + KYDLS L  + S G  LS  I   +  RF  +  VR+ YGL E     +  P+      A+ KV        +P+       + K+I+  + + + GP+ +GE+C+K      GY++  E  + + D EG+ HTGD+GYYD +   + ++++++LI +  Y+V P  LES LL   NI DA V G+PD                K   E+ + + + S++ + +R+RGG+ F+  +P+    K+    +R++L+
Sbjct:   80 IALCSENCEEFFIPVLAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTAIKTIVILDSKVDYRGYQSMDNFIKKN--TPQGFKGSSFKTVEVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRF-------SHARDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYLTCGFRIVMLTKFDEETFLKTLQDYKCSSVILVPTLFAILNRSELLDKYDLSNLVEIASGGAPLSKEIGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKV--------VPL------FKAKVIDLDTKKTL-GPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGKAIREILK 542          

HSP 2 Score: 60.8474 bits (146), Expect = 2.920e-8
Identity = 29/82 (35.37%), Postives = 47/82 (57.32%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKL 837
            + P  LE V++ HP + D  V     P  G LP A VVL+ G   T +E++ +  +++++   LRGG+ F D++PK   GK+
Sbjct:  451 VPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1325291650|ref|XP_023329934.1| (luciferin 4-monooxygenase-like [Eurytemora affinis])

HSP 1 Score: 318.161 bits (814), Expect = 1.792e-95
Identity = 149/278 (53.60%), Postives = 202/278 (72.66%), Query Frame = 0

Query:  318 IVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEP 579
            + D    TA LY WQ+R+L+ S  + KYDLS L+ +++ G +L+ T++ E+ E+ P +KYVREAYG+ E GL+T TYPK++   + +   +E P+DH++PVG PNM+T  KI++R +   V GPD QGE+C+K+ Q  +GY  ++ K L DS+GFFHTGDLGYYD +G I+++E+I NLI FWMYEV+P+VLE+RLL  N++VDAAVVGI DKENG                EENLTN +ESRLQDHER+RGGL++IQ+IPRDENWKV R VLQ + P
Sbjct:    7 LADPSISTAILYSWQVRMLTSSPLVEKYDLSHLRLLVTGGSLLNQTVKNEVFEKHPNIKYVREAYGIKEAGLLTYTYPKDRAKQMMSK--VEMPDDHLMPVGFPNMFTVFKIVDRVTGGPVSGPDMQGELCVKTCQLMVGYKGKEEKFL-DSDGFFHTGDLGYYDKEGAIHFMEEISNLISFWMYEVSPSVLEARLLCHNSVVDAAVVGIQDKENGQLPRAFVVLKSGHEETEENLTNFLESRLQDHERLRGGLHYIQHIPRDENWKVRRSVLQKFIP 281          

HSP 2 Score: 77.0258 bits (188), Expect = 7.275e-11
Identity = 34/99 (34.34%), Postives = 61/99 (61.62%), Query Frame = 0

Query:  748 LSYWLKVYINPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNVEKLSQ 846
            L+YWL V ++   LE  ++ HP + D +++   V G+G +PRA+VVL  G+    E+   +A+++L+    L GG+V  D+L +D +G ++ N+E L +
Sbjct:  382 LNYWLHVSVSTRYLEKQLLLHPGICDVAIRGVKVQGVGQVPRAYVVLNKGFTLNGEDFACWANSKLSWHHRLFGGVVMTDRLARDSSGAILVNLENLDK 480          

HSP 3 Score: 73.9442 bits (180), Expect = 7.317e-10
Identity = 38/94 (40.43%), Postives = 59/94 (62.77%), Query Frame = 0

Query:  748 LSYWLKVYINPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNV 841
            +S+W+   ++P+VLE  ++ H +V D +V        G LPRAFVVLKSG+E T E + +F  +RL D + LRGG+ +   +P+D N K+  +V
Sbjct:  183 ISFWM-YEVSPSVLEARLLCHNSVVDAAVVGIQDKENGQLPRAFVVLKSGHEETEENLTNFLESRLQDHERLRGGLHYIQHIPRDENWKVRRSV 275          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1325291645|ref|XP_023329933.1| (4-coumarate--CoA ligase 3-like [Eurytemora affinis])

HSP 1 Score: 234.958 bits (598), Expect = 6.857e-67
Identity = 118/303 (38.94%), Postives = 181/303 (59.74%), Query Frame = 0

Query:   12 MRNLTTP---ANMSRRGSSSSVGPPPLASSHQGRYKGLLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSD 311
            MRNL +    +  +RRGS +S+      +    +YKG++STPG  KFSAWKRET+IP+Q    ++L  +  +  NKVW+KD+T GR+ETY     +V+++  GL+KLG  K  V+CM  +N+VEYW+  LAAW+ G  VMPVNC I  D L  Q+ E  A  +VCD+ N ED L +      +KH+I++  +  A   +    L+  +         +S ++D  +L +TS        + HT+KSL++  FSP G +NH++DQ   + +  G WFFH +G Y+ AL++  G++++ LSEY+D
Sbjct:    1 MRNLYSRDAGSGETRRGSVASLSLT--VNPMHAKYKGIVSTPGFCKFSAWKRETEIPKQSFSSWILGNMGNQSKNKVWMKDITTGRSETYASAKIHVERVVKGLSKLGVEKGSVVCMWTTNFVEYWICCLAAWELGAAVMPVNCLIHTDKLATQLLETAAGYLVCDELNLEDALALKSKVSSIKHIILMDQDECAEDALCFRKLMNTEENVRVSLKPVSLEKDPIYLIWTSIKDE-PLLVEHTNKSLLSQMFSPSGTSNHWLDQNQSEVVFGGCWFFHTTGLYSFALSAALGLTLHVLSEYTD 300          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|919073666|ref|XP_013418047.1| (4-coumarate--CoA ligase 1 [Lingula anatina])

HSP 1 Score: 233.417 bits (594), Expect = 1.108e-63
Identity = 157/540 (29.07%), Postives = 264/540 (48.89%), Query Frame = 0

Query:   61 ETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-----GDSIACGNWFFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE----------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            +  IP   + +FLLA I  K  + V L     G+  T+ +V   V ++AS LT+LGFGK DV C+ C N  E+ L+ L     GG V   N     D L  Q+    A+ I+     +ED + +A+       +  +   G+A GC   ++L+ DDG   P  + ++  EDVA LP +S ++G    +M TH +++             + +++     GD++     FFH  G   + L  I Y   V  +S+++   F+E I   + +     P    LL++   +  YDLSCL+ ++ +   L      EL +RF T   V + YGL E        P   ++  A+             V +PN  T+ K+++ ++ + + G D+ GE+  +  Q   GYLN  +      D +G+ HTGD+G+YDS   +Y +E+++ LI +  ++V P  LE+ LL    I+DAAVVGIPD+E GE                + + + +  +L  ++R+RGG+ F+  IP+  + K+LR ++++
Sbjct:    9 DVAIPEVSLADFLLADIGGKYEDYVALVSAETGKEYTFNEVRFYVRRVASALTRLGFGKGDVFCIYCPNIPEFALMFLGVLAVGGVVTTANPMYRHDELIPQLKNTAAQYII----TFEDAVPIAQQAASEVGLKAVYVFGEAPGCRSFSELLADDGSMCPKYVPVNPKEDVAILPSSSGTTGLPKAVMLTHYNVVGNLIQ--------MRELVYRGKPGDALMGALPFFHSFGMLVIMLGGIKYKQKVVCISQFNPLLFLESIQKYRVRMLPCVPPMALLLAKHPIVDNYDLSCLEDILCAAAPLGVETERELKKRFNT--EVGQGYGLTETSPCVSASPPTDRHPGASG------------VLMPN--TECKVVDIETGKEL-GQDQDGELWFRGPQVMKGYLNNPQATAATVDKDGWLHTGDIGHYDSGHQLYIVERLKELIKYKGFQVPPAELEAVLLSHPAILDAAVVGIPDQEAGELPFAMVILKEGKKATKKEVADFVAGKLAPYKRLRGGVEFVDEIPKSASGKILRRLIRD 519          

HSP 2 Score: 69.3218 bits (168), Expect = 2.153e-8
Identity = 34/83 (40.96%), Postives = 50/83 (60.24%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE V++SHPA+ D +V        G LP A V+LK G +AT +E+  F   +LA    LRGG+ F D++PK  +GK++
Sbjct:  431 VPPAELEAVLLSHPAILDAAVVGIPDQEAGELPFAMVILKEGKKATKKEVADFVAGKLAPYKRLRGGVEFVDEIPKSASGKIL 513          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1176095429|ref|XP_020618278.1| (4-coumarate--CoA ligase-like [Orbicella faveolata])

HSP 1 Score: 230.335 bits (586), Expect = 2.510e-62
Identity = 151/509 (29.67%), Postives = 251/509 (49.31%), Query Frame = 0

Query:   89 DVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW--FFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYE 578
            D   GR+ +Y ++ +   + +S L K GF K DV+ +   N +EY ++       GG    VN       L+ Q+  + AK +V      E+  D A +T +   VIV+G   +A GC  ++ L+ DDG   P  + ++  EDV  LPF+S ++G S G+M TH +L+A      G      D +  D     N   F+H+     +    +  G  +  LS +   +F++ I + K   A L P  I  L++   + K+DLS +  V S    L   +   +L+R P VK +R+ +G+ E    +   P E K    +  +L           LPN+  + K++N  + E + GP++ GEIC+K      GY+N  E   +  DS+G+ HTGD+G+YD     + +++++ LI +  ++V P  LE+ L+   NI D AV+G+PD E GE              E +   +  R+   +++RGG+ FI+ IP+  + K+LR +L+N E
Sbjct:   59 DAVSGRSYSYSQLKNLTQRFSSALVKRGFSKDDVLAIYLPNVLEYPIIFYGVAFLGGVSTTVNPLYSAKELDRQLRISGAKYVVTIPPLAENAKDAAASTGV-ASVIVLG---EAPGCESLSSLLSDDGSAFPEHVQINTKEDVVALPFSSGTTGLSKGVMMTHHNLVA------GLVLLMDDNIFPDDAIVLNLLPFYHIYALLCMMGGCLEAGCKLVLLSRFEPVSFLKAIQEYKVTDAPLVPPLILFLAKHPLVDKFDLSSVVRVNSGAAPLGKELETAMLKRLPQVKRIRQGFGMTELSGASHCTPFEGKTKSGSVGLL-----------LPNL--ECKVVNPDTGE-ILGPNQDGEICVKGPLVMKGYINNPEATAKTIDSDGWLHTGDIGHYDKDEYFFIVDRLKELIKYKGFQVPPAELEALLISHPNIDDVAVIGVPDPEAGELPKAFVVRRGDVTPEEIIEFVAKRVLPQKKLRGGVEFIEQIPKSPSGKILRRMLRNQE 543          

HSP 2 Score: 65.4698 bits (158), Expect = 3.311e-7
Identity = 32/83 (38.55%), Postives = 50/83 (60.24%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP +DD +V     P  G LP+AFVV +   + T EEI+ F   R+     LRGG+ F +++PK  +GK++
Sbjct:  455 VPPAELEALLISHPNIDDVAVIGVPDPEAGELPKAFVVRRG--DVTPEEIIEFVAKRVLPQKKLRGGVEFIEQIPKSPSGKIL 535          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|919027210|ref|XP_013397407.1| (4-coumarate--CoA ligase 1 [Lingula anatina] >gi|919027212|ref|XP_013397408.1| 4-coumarate--CoA ligase 1 [Lingula anatina])

HSP 1 Score: 226.483 bits (576), Expect = 4.052e-61
Identity = 159/535 (29.72%), Postives = 265/535 (49.53%), Query Frame = 0

Query:   61 ETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQ 575
            +  IP   I +FLLA + +K  + + + D       TYG V ++V  I S LT+ GF K DV+C+ C N  E+ LV L     GG V   N +   + LE+Q+  A  + I+      E  + +A  TE+ K V V+G+   A GC P  +L+ D G   P  +N+S  +DVA LPF+S ++G   G+M TH +++A       + +       G     G   FFH  G   + + SI +  ++  + ++    F+  I   K +     P  +  +++  ++ KYDLSC+++++S+   L S    EL  R  T   + + YGL E G      P  +      A  +  PN            T+ K+++  + + + GP++ GE+  +  Q FIGYLN         D +G+ HTGD+G+YD    +Y  ++I+ +I F  ++VAP  LES L+    + DAAVVG PD+E G                E ++ + +  ++  ++R+RGG+  +++IP+ E+ K+LR  L+
Sbjct:    9 DVRIPWVSITDFLLADV-DKYADFIAVVDTQSDIKYTYGDVRNHVRSIGSALTRKGFRKGDVLCIYCPNVPEFPLVLLGTLAVGGIVTTCNPQYTAEELEKQMNLAHVQYIITVPECAEKAIRVAATTEI-KAVYVLGS---AQGCTPFTELLNDSGSAFPDDVNIS-PQDVALLPFSSGTTGLPKGVMLTHYNIVANLLQ---SRDFIYTGRPGVDFVLGYLPFFHGFGLLVINIGSIKFKQTIVCMRKFDFEDFLAAIEKYKIRYLFAAPPIVIRMAKHPSVAKYDLSCVETIMSASAPLGSDSEEELEARLGTCS-LTQGYGLTETGPFCSLPPLSRHLRRPGASGVLVPN------------TECKVVDPITKKEL-GPNQDGELWFRGPQVFIGYLNNPVATAATLDKDGWIHTGDIGHYDEDQYLYIKDRIKEMIKFKGFQVAPAELESVLVTHPAVEDAAVVGRPDQEAGELPMAFVVLKSGRMTTERDIMDFLAEKVAPYKRLRGGVVIVKSIPKTESGKILRRFLR 520          

HSP 2 Score: 66.2402 bits (160), Expect = 1.634e-7
Identity = 34/83 (40.96%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE V+++HPAV+D +V        G LP AFVVLKSG   T  +I+ F   ++A    LRGG+V    +PK  +GK++
Sbjct:  433 VAPAELESVLVTHPAVEDAAVVGRPDQEAGELPMAFVVLKSGRMTTERDIMDFLAEKVAPYKRLRGGVVIVKSIPKTESGKIL 515          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1126216807|ref|XP_019642430.1| (PREDICTED: probable 4-coumarate--CoA ligase 3 isoform X1 [Branchiostoma belcheri])

HSP 1 Score: 226.868 bits (577), Expect = 1.356e-60
Identity = 157/539 (29.13%), Postives = 265/539 (49.17%), Query Frame = 0

Query:   61 ETDIP-RQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQ----MIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG---------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            +  IP +Q++ EF+L    +   N V + D   GR+ T+ ++   + +++S LT+LGF ++DV+ +   N  EY +V  A    GG V  VN       L  QVT + A+ IV      E+      N   +K + +IG  G A GC P++DL+ DDG   P  + +   ED+A LP++S ++G   G+M TH S++A            +D+    +IG        FFH+ G   +    +  GV + ++  +   +F+  + + K       P     L++   +  YDLS +K V      + + I M L++R     + R+ YGL E   + +T  +E      +  VL           +PN  T+ K+I+ ++ E V GP E GE+C+   Q   GY N  E      D +G+  TGD+  YD  G  Y +++++ LI +  ++VAP  LE+ LLG   + DAAV+G+PD   G               E+++T+ +  R+  ++++RGG+ FI  IP+  + K+LR +L++
Sbjct:   79 DVHIPDKQHLTEFVLKDFDQYGDN-VAMVDGPTGRSYTFSELKDLIRRLSSALTRLGFKQQDVLALYSPNLPEYAIVLFAVTALGGIVTTVNPLYTVGELTNQVTHSGAQYIVTIADFAENARKAKSNCPNVKDIYIIG--GTAEGCKPLSDLLADDGSAMPSDVKIDPHEDIAMLPYSSGTTGLPKGVMLTHYSIIANLCQNLVKGVMLLDREEECLIGQL-----PFFHIYGLVVILFNCLMQGVRLVTVPRFEPESFLACVQNYKVTRILTVPPVAVFLAKHPIVDNYDLSLIKEVFCGAAPMGTEITMALMDRLKIPNH-RQGYGLTETSPI-VTIGREGVFVPGSFGVL-----------VPN--TKAKVIDTETGEAV-GPGEDGELCVLGPQVMKGYYNNPEATANTIDPDGWLRTGDIVRYDEDGNFYAVDRVKELIKYKGFQVAPAELEAVLLGHPGVQDAAVIGLPDDVAGELPKAFIVKKSDAVTEKDITDFVAERVAPYKKLRGGVVFIGEIPKTPSGKILRRMLKD 593          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1126216809|ref|XP_019642431.1| (PREDICTED: probable 4-coumarate--CoA ligase 3 isoform X2 [Branchiostoma belcheri])

HSP 1 Score: 226.868 bits (577), Expect = 1.411e-60
Identity = 157/539 (29.13%), Postives = 265/539 (49.17%), Query Frame = 0

Query:   61 ETDIP-RQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQ----MIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG---------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            +  IP +Q++ EF+L    +   N V + D   GR+ T+ ++   + +++S LT+LGF ++DV+ +   N  EY +V  A    GG V  VN       L  QVT + A+ IV      E+      N   +K + +IG  G A GC P++DL+ DDG   P  + +   ED+A LP++S ++G   G+M TH S++A            +D+    +IG        FFH+ G   +    +  GV + ++  +   +F+  + + K       P     L++   +  YDLS +K V      + + I M L++R     + R+ YGL E   + +T  +E      +  VL           +PN  T+ K+I+ ++ E V GP E GE+C+   Q   GY N  E      D +G+  TGD+  YD  G  Y +++++ LI +  ++VAP  LE+ LLG   + DAAV+G+PD   G               E+++T+ +  R+  ++++RGG+ FI  IP+  + K+LR +L++
Sbjct:   79 DVHIPDKQHLTEFVLKDFDQYGDN-VAMVDGPTGRSYTFSELKDLIRRLSSALTRLGFKQQDVLALYSPNLPEYAIVLFAVTALGGIVTTVNPLYTVGELTNQVTHSGAQYIVTIADFAENARKAKSNCPNVKDIYIIG--GTAEGCKPLSDLLADDGSAMPSDVKIDPHEDIAMLPYSSGTTGLPKGVMLTHYSIIANLCQNLVKGVMLLDREEECLIGQL-----PFFHIYGLVVILFNCLMQGVRLVTVPRFEPESFLACVQNYKVTRILTVPPVAVFLAKHPIVDNYDLSLIKEVFCGAAPMGTEITMALMDRLKIPNH-RQGYGLTETSPI-VTIGREGVFVPGSFGVL-----------VPN--TKAKVIDTETGEAV-GPGEDGELCVLGPQVMKGYYNNPEATANTIDPDGWLRTGDIVRYDEDGNFYAVDRVKELIKYKGFQVAPAELEAVLLGHPGVQDAAVIGLPDDVAGELPKAFIVKKSDAVTEKDITDFVTERVAPYKKLRGGVVFIGEIPKTPSGKILRRMLKD 593          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1176094477|ref|XP_020617773.1| (4-coumarate--CoA ligase 1-like [Orbicella faveolata])

HSP 1 Score: 221.476 bits (563), Expect = 2.271e-59
Identity = 145/508 (28.54%), Postives = 253/508 (49.80%), Query Frame = 0

Query:   89 DVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYV-DQMIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYE 578
            D T G   T+ ++ +     AS L K GF K DV+ +   N  EY +    A   GG V  VN     + LE Q+ + ++  +V      +     ++    +K +IVIG E  +  C+  ++L+EDDG + P +  +  D DV  +P++S ++G   G+M TH +++A     +G+    + +Q +  +I     FFH+ G   +  + ++ G  +     +    F++ + D+K   A + P  +  L++   + KYDLS ++ V      +   +   L++R P VK +R+ YG+ E  LVT   P+ ++   +   +L      ++ V    M                GP + GEICIK+     GYLN  E   R+ DSEG+  TGD+G+YD  G  + +++++ LI +  Y+VAP  LE+ L+   NI DAAV+G+PD+++GE              E++ + +  R+  H+++RGG+ F+  IP+  + K+LR  L+  E
Sbjct:   37 DGTSGHAYTFTQLKTLTKNFASSLVKRGFKKGDVLAIYLPNVPEYPIAFYGAGFIGGTVTTVNPLYTTEELERQLQDTQSSFLVTSPLFIDKARAASKAQGKIKKIIVIGDE-VSEDCLAFSNLLEDDGSSFPSQCTVDKD-DVVSMPYSSGTTGVPKGVMITHNNMIADTCIMEGSEIFTLTEQSVVMTILP---FFHIYGQLVMMGSGLHQGAKLVVQQRFEPEKFLKAMQDHKVTNAPIVPPIVLFLAKHPLVDKYDLSSVEEVTCGAAPMGEGLEEALIKRMPRVKRIRQGYGMTEV-LVTHVQPRGERKPGSVGVLLSNVESKIVDVTTGAML---------------GPHQDGEICIKAPTVMKGYLNNPEATARMIDSEGWARTGDIGHYDEDGHFFIVDRLKELIKYNAYQVAPAELEALLISHLNIDDAAVIGVPDEKSGELPKAFVVPKGEISPEDVVDFVAERVAPHKKLRGGVEFVDKIPKSASGKILRKDLRKRE 523          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|918996533|ref|XP_013390242.1| (probable 4-coumarate--CoA ligase 1 [Lingula anatina])

HSP 1 Score: 220.32 bits (560), Expect = 2.326e-58
Identity = 164/572 (28.67%), Postives = 284/572 (49.65%), Query Frame = 0

Query:   33 PPLASSHQG---RYKGLLSTPGV--VKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI--GDSIACGNWFFHMSG-FYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTV---KYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRL-FDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------ENLTNLMESRLQD--------HERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            P L+  H G   +++ LLST  V      +   E  +P     E ++ +  EK  N V L D   G T +Y  +   V + AS LT++GF K DV+ +   N  +Y  V +A    GG V  +N     D L  Q+  + + ++V    + ++ +        +K VIV G      G  P  +L+EDDG   P  ++++ DEDV  LP++S ++G   G+M THK+++A   + +   +H V   +   D +     FFH+ G   T+      GV + ++ +++ + ++ ++  ++  + ++ P     +++   I K+DLS ++ V S+   LS T    L E F TV     +++ YGL E   VT   P     S +              +G+P   T+ KI++ +SN   +GP ++GE+C++  Q   GY N          +G+FHTGD+GY D+ G ++  ++++ LI F  ++V P  LE+ +L +  ++D AV+G+ D+E GE        +    L+E  LQD        ++R+RGG+ F+Q IP+    K+LR +L+N
Sbjct:   43 PSLSLHHTGGNEKFR-LLSTSAVRSAVLQSPHPEIHVPDASFAEHMM-RDWEKYSNNVALVDGATGETVSYSMLKDRVIRCASALTRMGFKKGDVLALYAHNVPDYATVFIAVGVLGGTVTALNPLYTIDELSTQLKRSSSSILVVAPSSVKNAIAAKSQCPNVKEVIVFG---NVDGIRPFTNLLEDDGHAFPENVDINVDEDVLALPYSSGTTGLPKGVMLTHKNIVA---NVEQLRHHGVMGYVPLSDCLVGVLPFFHIYGQVVTLFTGLAAGVKIVTMPKFTLDDYLHIVHRHRATSLHVVPPIALRMAKYPDIHKFDLSSVRQVFSAAAPLSDT----LSEEFSTVTGLSDIKQCYGLTETSPVTHCCPPRSWKSGS--------------IGVPVPNTKHKIVDIESNTP-QGPMQEGELCVQGPQVMKGYANNPEATAECIKDGWFHTGDIGYLDADGHVFISDRLKELIKFKGFQVPPAELEAVILTNPAVLDVAVIGVDDEEAGELPKAFVVVKPHAQLLEIELQDYVKTKVAPYKRLRGGVEFVQEIPKTPTGKILRRMLKN 587          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1251794970|gb|PDH33281.1| (4-coumarate--CoA ligase family protein [OM182 bacterium MED-G28])

HSP 1 Score: 218.009 bits (554), Expect = 3.317e-58
Identity = 156/546 (28.57%), Postives = 269/546 (49.27%), Query Frame = 0

Query:   55 FSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNE----CGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDN--KRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYE 578
            F   K E  +P   +   +L+K+ E   +K  L D   GRT TY ++ + +  +A+GL + GF K DV  + C N  EY  + L     GG    +N     + L  Q  ++ AK+++      +  L  A+   + K + V+G   +A      ++L+ + G   PP++ +    D+  LP++S ++G S G+M TH++L     S +   +  ++ +I + +  G   FFH+ G   +   +IY GV++ ++  +    F+++I D K  T +L P  +  L++   + KYD S ++ + S    L S I     ER     Y  + YGL E    C + ++  P EK  ++                 LPN Y+  K+I+ ++   + G +E+GE+ IK     +GYLN D+  K   D +G+FH+GD+GY D  G  Y +++++ LI +  Y+VAP  LE+ L+  + I DAAV+  PD+E GE              E + + + + +  H++IR  L F+  IP+  + K+LR VL   E
Sbjct:    3 FRNDKPEIQVPNSDLTSIVLSKVDELG-DKAALIDGPTGRTLTYTQLRTQIRHLAAGLDQRGFKKGDVCAVFCPNIPEYATIFLGVAAVGGINTTINSLYSTNDLIHQFKDSGAKILITIPAFMDRALPAAQECGI-KEIFVLG---EAENATAFSELLNNPGI--PPKVTIDPKRDLVALPYSSGTTGLSKGVMLTHENL-----SSNMVLSESINPLIDEDLMVGVLPFFHIYGMVLILNLAIYRGVTLVTMPRFDLEQFLQIIEDYKISTLSLVPPLVLALAKHPLVDKYDTSSIRLISSGAAPLGSEIEEACAERLGCQIY--QGYGLTEVSGACHVNSVPVPAEKTGAVGRV--------------LPNTYS--KVIDTETGAEL-GINERGEVLIKGMHIMVGYLNNDDATKHCIDEDGWFHSGDIGYADEDGYFYIVDRLKELIKYKAYQVAPAELEALLVSHDAIADAAVIPSPDEEAGEIPKAFVVLKGDISAEEIMSWVANEVAPHKKIR-KLEFVDEIPKSASGKILRRVLVEQE 516