BLAST of aconitase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 1363.59 bits (3528), Expect = 0.000e+0
Identity = 656/895 (73.30%), Postives = 768/895 (85.81%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVL-NGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVS----GKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +PF  +L++ ++ G    K++D++SL P Y +LP+SIR+LLESAVRNCDNFQV E DV  IL W  N+      E+PFRP RVILQDFTGVPA+VDFAAMRDAV++LGGDP  INP CP+DLVIDHS+QVDF R   AL KNE+LEF+RNKERFTFLKWGA +F+NMLI+PPGSGIVHQVNLEYLARVCFTD+D+V+PDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLP+V+GY+ISG    LV STD+VLTITKHLRQ+GVVG+FVEFYGPG+  LSIADRATI+NMCPEYGAT+G FPVD  SI+YL+ TNR+++K+K I+ YL+   ++R+Y D S DPTFS++V LDL +VV S SGPKRP DRV+V+DMK DFL CLS  IGFKG+ +P +K   TVP +  + KE+++ HG+VLIAAITSCTNTSNPSVMLGAGL+AKKAV+KGL+V PYIKTSLSPGSGVVTYYLK SGVIPYLDALGFSIVGYG MTCIGNSGPLE+ V     KGDLVC GVLSGNRNFEGRI+  TRANYLASPPLVIAYAIAGRVDIDFE EALG DA+ +P++LRDIWP+R+EIQ VEKKY++P+MFE+VYSK+TTGN+RWN LEAP+G LYPWD  STYIKSPPFFD M++ELP P+K++NAL LLNL  S T DHISPAG IARN PAAR+L SK I+PREFNSYGSRRGNDA+MARGTFANIRLVNKF+ G+ GPKT+H+ S ++MDVFDAA EYKKEN+HL++LAG+DYGCGSSRDWAAKGPFL GVRAV+AESYERIHRSNLVGMGIVPLQY  GE+++SLG + KE+F+V +P +L PQQ L+++V       KSFK   RFDTELELTYYRHGGILNYMIRK+I+
Sbjct:    3 NPFDKILKNFEIGGK-TYKYYDVSSLDPSYHKLPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRG--KNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKVIS 894          

BLAST of aconitase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 869.381 bits (2245), Expect = 0.000e+0
Identity = 452/919 (49.18%), Postives = 623/919 (67.79%), Query Frame = 0

Query:   29 LLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQ--VLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFV--------------RTP-----------------DALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGP-LEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDAD-GKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEG-KLYPWDQNSTYIKSPPFFDGMTKELPA-PRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQV-SGKKSFKVLARFDTELELTYYRHGGILNYMIRK 909
            LL+D +V G  + K++D+  +   Y +LP+S+RVLLE AVR     Q  V +    +IL+                  RV+LQDFTGVPA++D AA+R++V + GG P++++  CP+DLV+DH VQVD+               +TP                 DAL  N+  EFERNKERF FLKW   AFKN+ ++PPG+G++HQVNLEYLARV      +++PDSVVGTD+HTTMINGLGV+GWGVG ++A+AVM G P+++  P+V+G +I G +    TSTD+VL ITK LRQ GV G FVEF+GP +  LS+ADRATI+NMCPEYGA VG+FP+D  +I YL Q+ RD + I+ +E Y+  V M R+ SD +++  F+ ++E+DL+ ++P +SGPKR  D+VS++++  DF + L    G KGYGL + +        ++G+   + HG VL+AAI+SCTNTSNPSVMLGAGL+AKKAVE GLSV   +KTSL+PGSGVVT YL+ESGV+PYL  LGF IVGYGC TC  NSGP L + + +AIK+G+LVCCG+LSGNRNFEGR+  D RANYLASP +VIAYAIAGR+DI+ + E LG ++  G+ +FL++IWPSR+EIQ +E+K VIP ++ +VYSK++ GN  W+ L  P     +PW  +STYIK P +     KE+ +  R  +N   LL L D++T+DHISPAGSI R+SPAA +L S G+ PRE+NSYG+RRGN  VM RGTF+N+RL N  + + GP TLH+PS     +F+A+E YK +   L  + G+++G G+SRDWA +GPFL GVRA++A S++  +RSNLV  GI+PLQ  S        L   E  ++ I  ++ P+  ++V V   ++SFK + R D + E   +  GG++  ++ K
Sbjct:   15 LLKDFQV-GERSYKYYDVTQI-QGYEELPYSLRVLLECAVRKRSETQGQVWKDVYERILR------------------RVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRS-SDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYI----KEIGSNARNWKNMRCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLLNKVGPYTLHLPSEVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQITS---ISYESLKGTECLSIDIRDNITPKCLIDVVVGKAERSFKAVVRVDNDYETDVFHAGGVIAQIVSK 905          

BLAST of aconitase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 140.584 bits (353), Expect = 4.408e-34
Identity = 151/522 (28.93%), Postives = 230/522 (44.06%), Query Frame = 0

Query:   89 QNQSVPSGVE-VPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFL-----KWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSV-VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDP----TFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRAN-YLASPPLVIAYAIAGRVDIDFETEAL 598
            +NQ +  G   +  RP RV +QD T   A++ F +         G P++     PS +  DH +Q       D L++ ++L    N E + FL     K+G   +K      PGSGI+HQ+ LE  A          +P  + VGTDSHT    GLG +  GVGG +A  VM   P  +  PKV+G K++GK+    +  DV+L +   L   G  G  VE++G GV S+S     TI NM  E GAT   FP + +   YL  TNR            +   +   Y +    P     + +VVE+DL ++ P ++GP  P                L+  IG  G    K           NG    +  G      I SCTN+S   +     ++ K+A+ KG+         ++PGS  +   ++  G+   +   G  ++   C  CIG          + I KG+     V S NRNF GR  A+   + ++ SP + +A ++AG +D D  ++A+
Sbjct:  661 KNQDIVRGESYLRLRPDRVAMQDATAQMAMLQFIS--------SGLPKV---AVPSTIHCDHLIQAQIEGKKD-LARAKDL----NAEVYNFLSTSGAKYGVGFWK------PGSGIIHQIILENYA----------FPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRS-----------DIANLADKYREVLLSPDGGCKYDQVVEIDLNTLEPHVNGPFTPD---------------LATPIGELGEKAKK-----------NGWPLEVKDG-----LIGSCTNSSYEDMARXTAII-KQALAKGMKSKSNF--YITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWD------RQNITKGEKNTI-VTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAI 1098          

BLAST of aconitase vs. SwissProt
Match: gi|2506131|sp|P09339.4|ACNA_BACSU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 957.207 bits (2473), Expect = 0.000e+0
Identity = 477/888 (53.72%), Postives = 637/888 (71.73%), Query Frame = 0

Query:   40 NLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFT---DSDIV-YPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPF-VLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNL------ADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKK----SFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +LK  + + +G +  +LP+SI+VLLES +R  D F + +  V  + KW   +     ++VPF+P+RVILQDFTGVPAVVD A++R A+  +GGDP+ INP+ P DLVIDHSVQVD   T DAL+ N +LEFERN ER+ FL W  KAF N   VPP +GIVHQVNLE+LA V      D ++V YPD++VGTDSHTTMING+GV+GWGVGGIEAEA MLGQP    +P+V+G K+ GK+ +  T+TD+ L +T+ LR+ GVVGKFVEF+GPG+A L +ADRATIANM PEYGAT G+FPVD ++++YL+ T RD   I  +EAY  + G+   Y+  ++DP F++VVE+DL+ +  +LSGPKRP D + +S M+  F   L +  G +G+GL  ++    + F +LNG+E V+  GA+ IAAITSCTNTSNP V++GAGL+AKKAVE GL V  Y+KTSL+PGS VVT YL  SG++PY+  LGF++VGYGC TCIGNSGPL   + EA+ K DL+   VLSGNRNFEGRIH   + NYLASPPLV+AYA+AG V+I+ +T+ +G   DG+ V+  DIWPS +EI A+ K+ V P +F + Y  +   N RWN++E  +  LY WD +STYI++PPFF+ M+ E   P KVE   PL  L       DSVTTDHISPAG+I +++PA ++L  KG++PR+FNSYGSRRGN  VM RGTFANIR+ N+   G  G  T + P+ EV  ++DA  +YK++   LV+LAG+DYG GSSRDWAAKG  L G+R V+AES+ERIHRSNLV MG++PLQ+  GENAD+LGLT KE   V +   ++P+  + V+   +     +F+ + RFD+E+E+ YYRHGGIL  ++R+ +
Sbjct:   30 SLKALEDSGIG-KVSKLPYSIKVLLESVLRQVDGFVIKKEHVENLAKWGTAEL--KDIDVPFKPSRVILQDFTGVPAVVDLASLRKAMAAVGGDPDKINPEIPVDLVIDHSVQVDKAGTEDALAVNMDLEFERNAERYKFLSWAKKAFNNYQAVPPATGIVHQVNLEFLASVVHAIEEDGELVTYPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPVPEVIGAKLVGKLPNGTTATDLALKVTQVLREKGVVGKFVEFFGPGIAELPLADRATIANMAPEYGATCGFFPVDEEALNYLRLTGRDPEHIDVVEAYCRSNGLF--YTPDAEDPQFTDVVEIDLSQIEANLSGPKRPQDLIPLSAMQETFKKQLVSPAGNQGFGLNAEEENKEIKFKLLNGEETVMKTGAIAIAAITSCTNTSNPYVLIGAGLVAKKAVELGLKVPNYVKTSLAPGSKVVTGYLVNSGLLPYMKELGFNLVGYGCTTCIGNSGPLSPEIEEAVAKNDLLITSVLSGNRNFEGRIHPLVKGNYLASPPLVVAYALAGTVNINLKTDPIGVGKDGQNVYFNDIWPSMDEINALVKQTVTPELFRKEYETVFDDNKRWNEIETTDEALYKWDNDSTYIQNPPFFEEMSVE---PGKVE---PLKGLRVVGKFGDSVTTDHISPAGAIGKDTPAGKYLQEKGVSPRDFNSYGSRRGNHEVMMRGTFANIRIKNQIAPGTEGGFTTYWPTGEVTSIYDACMKYKEDKTGLVVLAGKDYGMGSSRDWAAKGTNLLGIRTVIAESFERIHRSNLVFMGVLPLQFKQGENADTLGLTGKEVIEVDVDETVRPRDLVTVRAINEDGNVTTFEAVVRFDSEVEIDYYRHGGILQMVLREKM 906          

BLAST of aconitase vs. SwissProt
Match: gi|254806048|sp|A0JMA0.1|IREB2_XENTR (RecName: Full=Iron-responsive element-binding protein 2; Short=IRE-BP 2)

HSP 1 Score: 944.495 bits (2440), Expect = 0.000e+0
Identity = 448/756 (59.26%), Postives = 578/756 (76.46%), Query Frame = 0

Query:  156 VRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +  P+A  K+ E+EF RNKER  F KW  KAF N+ ++PP +G VHQVNLE+L+RV   +   +YPDSV+GTDSHTTM+NGLG++G GVGGIE+EA MLG PI++ LP+V+G +++G ++ L TS DVVL+ITKHL+Q GV G FVEF+G GV+ LS+ADR TIANMCPEYGATV +FPVDS ++ +LKQT  D   +   E YL AV ++R   +  Q P +S+V++++L S+VP +SGPKRP DR+SV DMK DF  CL+   G KG+ +P+ K +  VP      E+ +SHG V+IAA+TSCTN  NPSVML AGL+AKKAVE GL+V PYIKTSLSPGSG VTYYL  SGV+PYL  LGF I+GYGC  C+GN+ PL E +  AIK+G+LV CGV SGN++FEG   +   ANYLASPPLV+AYA+AG V+ID +TEALG++A G+ +FLRDIWPSREE+  VE+  VIP+MF E+  KI   N RWN L+APE  L+PWD  ST+I+SPPFF  + K  P  + +E A  LL L DSVTTDH+SPAGSI R SPAA++L  K + PREFNSYG+RRGNDAVM RGTFAN++L NK +G+ GPKT H+PS ++MDVFDAAE Y+K    L+I+AG+ YG G+SRDWAAKGPFL GVR V+AESYE+IH+ +LVGMGI PLQ++SGENA++LGL+AKE+++  +P DL P+  +EV+ +  K+F V+A FD E E+T+Y+HGGIL+Y+ RK +
Sbjct:  204 IAEPEAALKSLEIEFNRNKERLQFFKWCTKAFHNVAVIPPETGTVHQVNLEFLSRVVMEEKGFIYPDSVLGTDSHTTMVNGLGILGLGVGGIESEAAMLGVPITLTLPEVIGCELTGAINPLATSIDVVLSITKHLKQAGVAGTFVEFFGNGVSQLSVADRTTIANMCPEYGATVAFFPVDSVTLRHLKQTGVDVQSVSTFETYLQAVKLLRQ--ENVQQPEYSKVLQINLNSIVPYVSGPKRPQDRISVMDMKKDFEACLNEKTGLKGFQIPEKKQSIMVPVTYENSEYSLSHGCVVIAAVTSCTNNCNPSVMLTAGLLAKKAVEAGLTVKPYIKTSLSPGSGTVTYYLSASGVLPYLSKLGFDIIGYGCARCVGNTNPLPESIVTAIKEGELVACGVFSGNKHFEGNRCSCVCANYLASPPLVVAYALAGTVNIDLQTEALGENAQGEKIFLRDIWPSREEVLEVEETMVIPSMFSELKLKIEKQNTRWNLLDAPESTLFPWDLRSTFIRSPPFFHKLEKIPPPIQPIEKAHVLLYLGDSVTTDHMSPAGSIPRTSPAAKYLIQKNLIPREFNSYGARRGNDAVMTRGTFANMKLFNKLVGKTGPKTFHLPSGQIMDVFDAAELYQKAEIPLIIIAGKKYGLGNSRDWAAKGPFLLGVRVVIAESYEKIHKDHLVGMGIAPLQFLSGENAETLGLSAKEQYSFSLPVDLTPRHKIEVKTNTGKTFHVIAAFDNEAEVTFYKHGGILSYVARKYL 957          

HSP 2 Score: 143.28 bits (360), Expect = 1.275e-33
Identity = 67/135 (49.63%), Postives = 92/135 (68.15%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASL-GPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVR 157
            +PF +L+  L   G  +  FF+++ L   EY  LP+ IRV+LE+ VRNCD   V E D   IL W   ++     E+PF PARV+LQDFTG+PA+VDFAAMRDA+ + G DP+ +NP CP+DL+ DHS+Q+DF +
Sbjct:    4 NPFHYLVEPLS--GTSDKTFFNVSKLKATEYDSLPYCIRVVLEAVVRNCDGVLVKEQDAFNILNW---KATCEFKEIPFLPARVMLQDFTGIPAMVDFAAMRDAISKFGRDPKQVNPACPTDLIADHSLQLDFTK 133          

BLAST of aconitase vs. SwissProt
Match: gi|30173505|sp|P70920.2|ACNA_BRADU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 929.472 bits (2401), Expect = 0.000e+0
Identity = 459/881 (52.10%), Postives = 617/881 (70.03%), Query Frame = 0

Query:   55 QLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSD------------IVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQA------GPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVH-IPADLKPQQTLEVQ-VSGKKSFK---VLARFDTELELTYYRHGGILNYMIRKMIA 912
            +LP+S++VLLE+ +RN D   V + D++ + KW + +S+    E+ FRPARV++QDFTGVPAVVD AAMR+A+Q+LGGD E INP  P DLVIDHSV V+F     A +KN   E+++N+ER+ FLKWG  AF N  +VPPG+GI HQVNLEYL++  +T  +            + YPDS+VGTDSHTTM+NGL V+GWGVGGIEAEA MLGQP+SM+LP V+G+K+ G M   VT+TD+VLT+T+ LR++GVVGKFVEF+GPG+  LS+AD+ATIANM PEYGAT G+FPVD+ +IDYLK + R A ++  ++AY  A G+ R  +  S DP F+E + LDLA VVPS++GPKRP  R+++  +   F   L+    +K    P  +      F + GK++ I HG V+IAAITSCTNTSNPSV++GAGL+A+ A  KGL   P++KTSL+PGS VV  YL +SG+  +LD +GF++VG+GC TCIGNSGPL E +S++I    +V   VLSGNRNFEGR+  D +ANYLASPPLV+A+A+AG V  +   E LG+  DGKPV+L+DIWP+ +EI A  KK+V  ++F++ Y+ +  G+  W K++  E + Y W+ +STY+++PP+F+GM KE      +  A  L    D +TTDHISPAGSI   SPA ++L    + P +FN YG+RRGN  VM RGTFANIR+ N  +  A      G  T H P  E M ++DAA +Y++E   LV+ AG +YG GSSRDWAAKG  L GVRAV+ +S+ERIHRSNLVGMG++PL +  G +  SLGL   E+ T+  +  DLKP+Q L  + VSG  S +   +L R DT  EL YYR+GGIL+Y++RK+ A
Sbjct:   38 KLPYSMKVLLENLLRNEDGRSVKKADIVAVSKWLRKKSLEH--EIAFRPARVLMQDFTGVPAVVDLAAMRNAMQKLGGDAEKINPLVPVDLVIDHSVIVNFFGDNKAFAKNVTEEYKQNQERYEFLKWGQAAFSNFSVVPPGTGICHQVNLEYLSQTVWTKKEKMTVGKKTGTFEVAYPDSLVGTDSHTTMVNGLAVLGWGVGGIEAEACMLGQPLSMLLPNVVGFKLKGAMKEGVTATDLVLTVTQMLRKLGVVGKFVEFFGPGLDHLSVADKATIANMAPEYGATCGFFPVDAAAIDYLKTSGRAAPRVALVQAYAKAQGLFR--TAKSADPVFTETLTLDLADVVPSMAGPKRPEGRIALPSVAEGFSVALANE--YKKTEEPAKR------FAVEGKKYEIGHGDVVIAAITSCTNTSNPSVLIGAGLLARNAAAKGLKAKPWVKTSLAPGSQVVAAYLADSGLQAHLDKVGFNLVGFGCTTCIGNSGPLPEEISKSINDNGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAHALAGSVTKNLAVEPLGEGKDGKPVYLKDIWPTSKEINAFMKKFVTASIFKKKYADVFKGDTNWRKIKTVESETYRWNMSSTYVQNPPYFEGMKKEPEPVTDIVEARILAMFGDKITTDHISPAGSIKLTSPAGKYLSEHQVRPADFNQYGTRRGNHEVMMRGTFANIRIKNFMLKGADGNIPEGGLTKHWPDGEQMSIYDAAMKYQQEQVPLVVFAGAEYGNGSSRDWAAKGTRLLGVRAVICQSFERIHRSNLVGMGVLPLTFEEGTSWSSLGLKGDEKVTLRGLVGDLKPRQKLTAEIVSGDGSLQRVSLLCRIDTLDELDYYRNGGILHYVLRKLAA 906          

BLAST of aconitase vs. SwissProt
Match: gi|38604821|sp|Q8CPC2.1|ACNA_STAES (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 477/892 (53.48%), Postives = 620/892 (69.51%), Query Frame = 0

Query:   43 FFDLASLGPE----YGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFT-DSD---IVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLN-GKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNL------ADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKK------SFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            ++DL +L  +      +LP+SIRVLLES +R  D+F V+  D IK L    N       EVPF+P+RVILQDFTGVPAVVD A++R A+  +GGD   INP+ P DLVIDHSVQVD    P+AL +N +LEFERN ER+ FL W  KAF N   VPP +GIVHQVNLEYLA V    D D     +PD++VGTDSHTTMING+GV+GWGVGGIEAEA MLGQP    +P+V+G +++  +    T+TD+ L +T+ LR+ GVVGKFVEF+GPGV  L +ADRATIANM PEYGAT G+FPVD +S+ Y+K T RD   I+ ++ YL    M   +    +DP +++V++LDL++V  SLSGPKRP D + +SDMK +F   ++A  G +G+GL + +         N G +  +  G + IAAITSCTNTSNP VMLGAGL+AKKAVEKGL V  ++KTSL+PGS VVT YL++SG+  YLD LGF++VGYGC TCIGNSGPL   + +A+   DL+   VLSGNRNFEGRIH   +ANYLASP LV+AYA+AG VDID + E +G+  DGK V+L+DIWPS +E+     K V P +F E Y  +   N+ WN+++  +  LY +D NSTYI++P FF G++KE   P K+E   PL +L       DSVTTDHISPAG+I +++PA ++L    +  R FNSYGSRRGN  VM RGTFANIR+ N+   G  G  T + P+ E+M ++DAA +YK++   LV+LAG DYG GSSRDWAAKG  L GV+ V+A+SYERIHRSNLV MG++PLQ+  GE+A++LGL  KEE +V I  D++P     V V+ KK      +FK + RFD+ +EL YYRHGGIL  ++R  +A
Sbjct:   22 YYDLQTLEEKGLAKISKLPYSIRVLLESVLRQEDDF-VITDDHIKALSKFGNAGNEG--EVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMF--FDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINFNDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKE---PGKIE---PLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTGEIMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVDINEDVQPHDL--VNVTAKKENGEIINFKAIVRFDSLVELDYYRHGGILQMVLRNKLA 900          

BLAST of aconitase vs. SwissProt
Match: gi|81674773|sp|Q5HPJ0.1|ACNA_STAEQ (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 477/892 (53.48%), Postives = 620/892 (69.51%), Query Frame = 0

Query:   43 FFDLASLGPE----YGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFT-DSD---IVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLN-GKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNL------ADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKK------SFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            ++DL +L  +      +LP+SIRVLLES +R  D+F V+  D IK L    N       EVPF+P+RVILQDFTGVPAVVD A++R A+  +GGD   INP+ P DLVIDHSVQVD    P+AL +N +LEFERN ER+ FL W  KAF N   VPP +GIVHQVNLEYLA V    D D     +PD++VGTDSHTTMING+GV+GWGVGGIEAEA MLGQP    +P+V+G +++  +    T+TD+ L +T+ LR+ GVVGKFVEF+GPGV  L +ADRATIANM PEYGAT G+FPVD +S+ Y+K T RD   I+ ++ YL    M   +    +DP +++V++LDL++V  SLSGPKRP D + +SDMK +F   ++A  G +G+GL + +         N G +  +  G + IAAITSCTNTSNP VMLGAGL+AKKAVEKGL V  ++KTSL+PGS VVT YL++SG+  YLD LGF++VGYGC TCIGNSGPL   + +A+   DL+   VLSGNRNFEGRIH   +ANYLASP LV+AYA+AG VDID + E +G+  DGK V+L+DIWPS +E+     K V P +F E Y  +   N+ WN+++  +  LY +D NSTYI++P FF G++KE   P K+E   PL +L       DSVTTDHISPAG+I +++PA ++L    +  R FNSYGSRRGN  VM RGTFANIR+ N+   G  G  T + P+ E+M ++DAA +YK++   LV+LAG DYG GSSRDWAAKG  L GV+ V+A+SYERIHRSNLV MG++PLQ+  GE+A++LGL  KEE +V I  D++P     V V+ KK      +FK + RFD+ +EL YYRHGGIL  ++R  +A
Sbjct:   22 YYDLQTLEEKGLAKISKLPYSIRVLLESVLRQEDDF-VITDDHIKALSKFGNAGNEG--EVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMF--FDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINFNDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKE---PGKIE---PLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTGEIMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVDINEDVQPHDF--VNVTAKKENGEIINFKAIVRFDSLVELDYYRHGGILQMVLRNKLA 900          

BLAST of aconitase vs. nr
Match: gi|1227979290|ref|XP_021921229.1| (cytoplasmic aconitate hydratase-like isoform X2 [Zootermopsis nevadensis] >gi|1227979292|ref|XP_021921230.1| cytoplasmic aconitate hydratase-like isoform X3 [Zootermopsis nevadensis])

HSP 1 Score: 1384.39 bits (3582), Expect = 0.000e+0
Identity = 669/882 (75.85%), Postives = 763/882 (86.51%), Query Frame = 0

Query:   30 LRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            L+ L V+G    KFFDL SLG  Y +LPFSIRVLLESAVRNCDNFQV E DV  IL W ++Q+VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV++LGGDP+ INP CPSDLVIDHSVQVDFVRTPDAL KN++LEFERNKERF FLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV F    ++YPDSVVGTDSHTTMINGLGV+GWGVGGIEAEAVMLGQ ISM+LPKV+GYKI+G+++   TSTD+VLTITKHLRQ+GVVGKFVEF+G GVA LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNR   K++ +E YL +V M RNY+D S+DP FSEVV LDLA+VV S+SGPKRPHDRV V DM TDF  CL+  IGFKG+GLP+DKL  +  F+  G+E+V+ HG+V+IAAITSCTNTSNPSVMLGAGL+AKKA++ GL+V PYIKTSLSPGSGVVTYYLKESGVIPYL ALGF IVGYGCMTCIGNSGPL E ++EAI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFE E LG   DGKPVFL +IWP R EI  VE+KYVIP MF EVY+KI TG+  W  L+APEGKLYPWD +STYIK+PPFF+ MTKELP    +E A  LLNL DSVTTDHISPAGSIARNSPAAR+L ++G+TPREFNSYGSRRGNDAVMARGTFANIRLVNKF+GQAGPKT+HIPS E+MDVFDAAE Y+KE   L+ L G+DYG GSSRDWAAKGPFL G+RAV+AESYERIHRSNLVGMGIVPLQY+ G+NA+SLGLT KE++T+ IP +++P Q L V++   KSF+V+ RFDTE++LTY+RHGGILNYMIRK++
Sbjct:   48 LKKLSVDGK-EYKFFDLPSLGSSYDKLPFSIRVLLESAVRNCDNFQVKEQDVEAILDWEKSQAVPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPDRINPVCPSDLVIDHSVQVDFVRTPDALQKNQDLEFERNKERFMFLKWGAKAFQNMLIVPPGSGIVHQVNLEYLARVVFDSEGVLYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQAISMLLPKVVGYKITGQLNQYATSTDLVLTITKHLRQLGVVGKFVEFFGSGVAELSIADRATISNMCPEYGATVGFFPVDKNSLAYLRQTNRSENKVRLMEEYLKSVRMFRNYTDESEDPVFSEVVTLDLATVVSSVSGPKRPHDRVPVVDMPTDFRQCLTNKIGFKGFGLPEDKLNASGSFIHEGREYVLHHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAIDAGLTVLPYIKTSLSPGSGVVTYYLKESGVIPYLTALGFDIVGYGCMTCIGNSGPLPESIAEAIEKNDLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFEKEPLGHSPDGKPVFLHEIWPLRNEIHDVEQKYVIPAMFREVYAKIETGSSNWANLKAPEGKLYPWDPSSTYIKNPPFFEAMTKELPNIEPIEEARVLLNLGDSVTTDHISPAGSIARNSPAARYLAARGLTPREFNSYGSRRGNDAVMARGTFANIRLVNKFVGQAGPKTIHIPSGEMMDVFDAAERYRKEKVPLIALVGKDYGSGSSRDWAAKGPFLLGLRAVIAESYERIHRSNLVGMGIVPLQYLEGQNAESLGLTGKEKYTIRIPENIRPLQELTVELVNGKSFQVVVRFDTEVDLTYFRHGGILNYMIRKIL 928          

BLAST of aconitase vs. nr
Match: gi|758375084|gb|AJO69941.1| (IRP1A [Lepeophtheirus salmonis])

HSP 1 Score: 1383.62 bits (3580), Expect = 0.000e+0
Identity = 663/895 (74.08%), Postives = 776/895 (86.70%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVL-NGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVS----GKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +PF  +L++ ++ G    K++D++SL P Y +LP+SIR+LLESAVRNCDNFQV E DV  IL W  N+      E+PFRP RVILQDFTGVPA+VDFAAMRDAV++LGGDP  INP CP+DLVIDHS+QVDF R   AL KNE+LEF+RNKERFTFLKWGA +F+NMLI+PPGSGIVHQVNLEYLARVCFTD+D+V+PDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLP+V+GY+ISG    LV STD+VLTITKHLRQ+GVVG+FVEFYGPG+  LSIADRATI+NMCPEYGAT+GYFPVD  SI+YL+ TNR+++K+K I+ YL+   ++R+Y D S DPTFS++V LDL +VV S SGPKRP DRV+V+DMK DFL CLS  IGFKG+ +P +K   TVP +  + KE+++ HG+VLIAAITSCTNTSNPSVMLGAGL+AKKAV+KGL+V PYIKTSLSPGSGVVTYYLK SGVIPYLDALGFSIVGYGCMTCIGNSGPLE+ V E + KGDLVC GVLSGNRNFEGRI+  TRANYLASPPLVIAYAIAGRVDIDFE EALG DA+ +P++LRDIWP+R+EIQ VEKKY++P+MFE+VYSK+TTGN+RWN LEAP+G LYPWD  STYIKSPPFFD M++ELP P+K++NAL LLNL DS TTDHISPAGSIARNSPAAR+L SK I+PREFNSYGSRRGNDA+MARGTFANIRLVNKF+ G+ GPKT+H+ S ++MDVFDAA EYKKEN+HL++LAG+DYGCGSSRDWAAKGPFL GVRAV+AESYERIHRSNLVGMGIVPLQY  GE+++SLG + KE+F+V +P +L PQQ L+++V       KSFK   RFDTELELTYYRHGGILNYMIRK+I+
Sbjct:    3 NPFDKILKNFEIGGK-TYKYYDVSSLDPSYHKLPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRG--KNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGYFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGCMTCIGNSGPLEDYVVEGVNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGDSFTTDHISPAGSIARNSPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKVIS 894          

BLAST of aconitase vs. nr
Match: gi|1227979288|ref|XP_021921228.1| (cytoplasmic aconitate hydratase-like isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 1373.61 bits (3554), Expect = 0.000e+0
Identity = 669/900 (74.33%), Postives = 763/900 (84.78%), Query Frame = 0

Query:   30 LRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRT------------------PDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            L+ L V+G    KFFDL SLG  Y +LPFSIRVLLESAVRNCDNFQV E DV  IL W ++Q+VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV++LGGDP+ INP CPSDLVIDHSVQVDFVRT                  PDAL KN++LEFERNKERF FLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV F    ++YPDSVVGTDSHTTMINGLGV+GWGVGGIEAEAVMLGQ ISM+LPKV+GYKI+G+++   TSTD+VLTITKHLRQ+GVVGKFVEF+G GVA LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNR   K++ +E YL +V M RNY+D S+DP FSEVV LDLA+VV S+SGPKRPHDRV V DM TDF  CL+  IGFKG+GLP+DKL  +  F+  G+E+V+ HG+V+IAAITSCTNTSNPSVMLGAGL+AKKA++ GL+V PYIKTSLSPGSGVVTYYLKESGVIPYL ALGF IVGYGCMTCIGNSGPL E ++EAI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFE E LG   DGKPVFL +IWP R EI  VE+KYVIP MF EVY+KI TG+  W  L+APEGKLYPWD +STYIK+PPFF+ MTKELP    +E A  LLNL DSVTTDHISPAGSIARNSPAAR+L ++G+TPREFNSYGSRRGNDAVMARGTFANIRLVNKF+GQAGPKT+HIPS E+MDVFDAAE Y+KE   L+ L G+DYG GSSRDWAAKGPFL G+RAV+AESYERIHRSNLVGMGIVPLQY+ G+NA+SLGLT KE++T+ IP +++P Q L V++   KSF+V+ RFDTE++LTY+RHGGILNYMIRK++
Sbjct:   48 LKKLSVDGK-EYKFFDLPSLGSSYDKLPFSIRVLLESAVRNCDNFQVKEQDVEAILDWEKSQAVPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPDRINPVCPSDLVIDHSVQVDFVRTHRPDLDMNGQVAECQYSKPDALQKNQDLEFERNKERFMFLKWGAKAFQNMLIVPPGSGIVHQVNLEYLARVVFDSEGVLYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQAISMLLPKVVGYKITGQLNQYATSTDLVLTITKHLRQLGVVGKFVEFFGSGVAELSIADRATISNMCPEYGATVGFFPVDKNSLAYLRQTNRSENKVRLMEEYLKSVRMFRNYTDESEDPVFSEVVTLDLATVVSSVSGPKRPHDRVPVVDMPTDFRQCLTNKIGFKGFGLPEDKLNASGSFIHEGREYVLHHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAIDAGLTVLPYIKTSLSPGSGVVTYYLKESGVIPYLTALGFDIVGYGCMTCIGNSGPLPESIAEAIEKNDLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFEKEPLGHSPDGKPVFLHEIWPLRNEIHDVEQKYVIPAMFREVYAKIETGSSNWANLKAPEGKLYPWDPSSTYIKNPPFFEAMTKELPNIEPIEEARVLLNLGDSVTTDHISPAGSIARNSPAARYLAARGLTPREFNSYGSRRGNDAVMARGTFANIRLVNKFVGQAGPKTIHIPSGEMMDVFDAAERYRKEKVPLIALVGKDYGSGSSRDWAAKGPFLLGLRAVIAESYERIHRSNLVGMGIVPLQYLEGQNAESLGLTGKEKYTIRIPENIRPLQELTVELVNGKSFQVVVRFDTEVDLTYFRHGGILNYMIRKIL 946          

BLAST of aconitase vs. nr
Match: gi|1183238293|ref|XP_020710808.1| (cytoplasmic aconitate hydratase-like [Athalia rosae])

HSP 1 Score: 1359.36 bits (3517), Expect = 0.000e+0
Identity = 641/888 (72.18%), Postives = 756/888 (85.14%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +P+ +LL+ +K+ G  + +++D+ + G +Y +LPFSIRVLLESAVRNCD FQV ++DV KIL+W  NQ +P GVEV F+PARVILQDFTGVPAVVDFAAMRDAV++LGGDP+ INP CPSDLVIDHSVQVDF R  +AL KNE+LEFERNKERF FLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARV F  + ++YPDSVVGTDSHTTMINGLGV+GWG GGIEAEAVMLGQ ISM+LP V+GYK+ G ++  VTSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA LSIADRATI+NMCPEYGATVG+F VD QS+ YL+QT+R    +K IE YL  V M+RNY+D+SQDP FSEVV LDL++VV S+SGPKRPHDRVSVS+MK DF +CLSA IGFKG+GL  +KL     F   GK++ ++HGAV+IAAITSCTNTSNPSVMLGAGL+A+KAV+ GL+VAPYIKTSLSPGSGVVTYYLKESGVIP L  LGF +VGYGCMTCIGNSGPL + + +AI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAG V IDFE E LG  ADGK ++LRD+WP+R EIQAVE+K+VIP MF+EVYSKI +G+  W+ L+AP G+LYPWD  STYIK PP+FDG+TK +P    +  A  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+ P+EFNSYGSRRGNDAVMARGTFANIRLVNKFIG+AGP+T++IP+NE MD+FDAAE+Y ++N  L+ L G++YG GSSRDWAAKGPFLQG++AV+AESYERIHRSNLVGMGI+PLQY+ GE+A+SLGLT  E + + IP D +P QT+EV     K F VL RFDTE++LTY+RHGGILNYMIR M+
Sbjct:    6 NPYKNLLKTIKI-GETDCQYYDVTNFGDKYDKLPFSIRVLLESAVRNCDEFQVKKSDVEKILEWETNQKIPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPDKINPICPSDLVIDHSVQVDFTRGDNALKKNEDLEFERNKERFMFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVVFDANGLLYPDSVVGTDSHTTMINGLGVLGWGCGGIEAEAVMLGQAISMLLPNVVGYKLEGVLNQYVTSTDLVLTITKNLRQLGVVGKFVEFFGPGVAELSIADRATISNMCPEYGATVGFFAVDEQSLAYLRQTSRSDKHVKYIEEYLRTVKMLRNYNDSSQDPVFSEVVTLDLSTVVSSVSGPKRPHDRVSVSEMKKDFTSCLSAKIGFKGFGLKPEKLKVEATFQFEGKDYKLNHGAVVIAAITSCTNTSNPSVMLGAGLLAQKAVKAGLTVAPYIKTSLSPGSGVVTYYLKESGVIPSLTQLGFDVVGYGCMTCIGNSGPLPDSMIDAIEKNDLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGTVLIDFEAEPLGYRADGKAIYLRDVWPTRAEIQAVEQKHVIPAMFKEVYSKIESGSGSWSSLQAPGGQLYPWDDESTYIKHPPYFDGLTKTIPPTNPIVKARVLLNLGDSVTTDHISPAGSIARNSPAARYLAKRGLKPKEFNSYGSRRGNDAVMARGTFANIRLVNKFIGKAGPRTIYIPTNEEMDIFDAAEKYAEDNVPLIALVGKEYGSGSSRDWAAKGPFLQGIKAVIAESYERIHRSNLVGMGIIPLQYLPGESAESLGLTGLELYNISIPDDYRPGQTIEVTTDDGKKFNVLVRFDTEVDLTYFRHGGILNYMIRTMV 892          

BLAST of aconitase vs. nr
Match: gi|939243160|ref|XP_014240878.1| (cytoplasmic aconitate hydratase-like isoform X2 [Cimex lectularius])

HSP 1 Score: 1350.5 bits (3494), Expect = 0.000e+0
Identity = 651/892 (72.98%), Postives = 757/892 (84.87%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            S  +P+  LL+ L V G     ++DL  LGP+YG+LP+S+RVLLESAVRNCDNFQV E DV  IL W  NQ V  GVEVPF+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDF RT DAL KNEELEFERNKERFTFLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV FTD   +YPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQ ISM++P+V+GYK+ G++    TSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA+LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNRDA K++A+E YL AVG++R++ D+ +DP FS+V+ELDL++VV  +SGPKRP D+VSV+DMK +F  CL+  +GF G+GL  + ++ T  F+  GKE+ I HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL V  Y+KTSLSPGSGVVT+YL+ESGVIPYL+ LGFSIVG+GCMTCIGNSGPL + + +AI+K +LVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFETE LG   DGK VFLRD+WPSR EI  VEK  VIP MF +VYS+I  G++ W  L AP+GKLYPWDQ STYIKSPPFF GMTKELP  + ++NA  LL L DSVTTDHISPAGSIARNSPAARFL S+G+TPREFNSYGSRRGND +MARGTFANIRLVN F+   GP+T+H PSNE +DVFDAAE YK+E   L+++ G+DYG GSSRDWAAKGPFL G++AV+AESYERIHRSNLVGMG+VPLQ+++G+NA SLGLT KE F++ IP+DL+P QT+ VQ    KSF V  RFDTE++L YYRHGGILNYMIRKM+A
Sbjct:    2 SGSNPYDKLLKSLSVGGV-TYNYYDLQGLGPKYGKLPYSVRVLLESAVRNCDNFQVKEKDVENILNWEVNQKVEGGVEVPFKPARVILQDFTGVPAVVDFAAMRDAVKSLGGDPDKINPICPSDLVIDHSVQVDFSRTNDALQKNEELEFERNKERFTFLKWGAKAFRNMLIVPPGSGIVHQVNLEYLARVVFTDGSTLYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQAISMLIPQVVGYKLVGELSQYATSTDLVLTITKNLRQLGVVGKFVEFFGPGVANLSIADRATISNMCPEYGATVGFFPVDVNSLQYLRQTNRDAVKVQAVEQYLKAVGLLRDFKDSGEDPVFSQVIELDLSTVVTCISGPKRPQDKVSVTDMKVEFAKCLTNKVGFNGFGLSPEVMSKTGKFIHEGKEYTIRHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLEVPAYVKTSLSPGSGVVTHYLRESGVIPYLNKLGFSIVGFGCMTCIGNSGPLPDYMVDAIEKNELVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFETEPLGVGVDGKQVFLRDVWPSRTEIHHVEKVTVIPGMFRDVYSRIENGSNSWRSLSAPDGKLYPWDQKSTYIKSPPFFAGMTKELPKAQPIKNARVLLLLGDSVTTDHISPAGSIARNSPAARFLASRGLTPREFNSYGSRRGNDDIMARGTFANIRLVNHFVSAPGPRTVHFPSNEELDVFDAAERYKQEGTPLILIVGKDYGSGSSRDWAAKGPFLLGIKAVIAESYERIHRSNLVGMGVVPLQFLNGQNAKSLGLTGKEVFSIDIPSDLRPLQTVSVQTDAGKSFDVTVRFDTEVDLLYYRHGGILNYMIRKMLA 892          

BLAST of aconitase vs. nr
Match: gi|939243158|ref|XP_014240876.1| (cytoplasmic aconitate hydratase-like isoform X1 [Cimex lectularius])

HSP 1 Score: 1350.5 bits (3494), Expect = 0.000e+0
Identity = 650/892 (72.87%), Postives = 757/892 (84.87%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +  +P+  LL+ L V G     ++DL  LGP+YG+LP+S+RVLLESAVRNCDNFQV E DV  IL W  NQ V  GVEVPF+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDF RT DAL KNEELEFERNKERFTFLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV FTD   +YPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQ ISM++P+V+GYK+ G++    TSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA+LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNRDA K++A+E YL AVG++R++ D+ +DP FS+V+ELDL++VV  +SGPKRP D+VSV+DMK +F  CL+  +GF G+GL  + ++ T  F+  GKE+ I HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL V  Y+KTSLSPGSGVVT+YL+ESGVIPYL+ LGFSIVG+GCMTCIGNSGPL + + +AI+K +LVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFETE LG   DGK VFLRD+WPSR EI  VEK  VIP MF +VYS+I  G++ W  L AP+GKLYPWDQ STYIKSPPFF GMTKELP  + ++NA  LL L DSVTTDHISPAGSIARNSPAARFL S+G+TPREFNSYGSRRGND +MARGTFANIRLVN F+   GP+T+H PSNE +DVFDAAE YK+E   L+++ G+DYG GSSRDWAAKGPFL G++AV+AESYERIHRSNLVGMG+VPLQ+++G+NA SLGLT KE F++ IP+DL+P QT+ VQ    KSF V  RFDTE++L YYRHGGILNYMIRKM+A
Sbjct:   11 TGSNPYDKLLKSLSVGGV-TYNYYDLQGLGPKYGKLPYSVRVLLESAVRNCDNFQVKEKDVENILNWEVNQKVEGGVEVPFKPARVILQDFTGVPAVVDFAAMRDAVKSLGGDPDKINPICPSDLVIDHSVQVDFSRTNDALQKNEELEFERNKERFTFLKWGAKAFRNMLIVPPGSGIVHQVNLEYLARVVFTDGSTLYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQAISMLIPQVVGYKLVGELSQYATSTDLVLTITKNLRQLGVVGKFVEFFGPGVANLSIADRATISNMCPEYGATVGFFPVDVNSLQYLRQTNRDAVKVQAVEQYLKAVGLLRDFKDSGEDPVFSQVIELDLSTVVTCISGPKRPQDKVSVTDMKVEFAKCLTNKVGFNGFGLSPEVMSKTGKFIHEGKEYTIRHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLEVPAYVKTSLSPGSGVVTHYLRESGVIPYLNKLGFSIVGFGCMTCIGNSGPLPDYMVDAIEKNELVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFETEPLGVGVDGKQVFLRDVWPSRTEIHHVEKVTVIPGMFRDVYSRIENGSNSWRSLSAPDGKLYPWDQKSTYIKSPPFFAGMTKELPKAQPIKNARVLLLLGDSVTTDHISPAGSIARNSPAARFLASRGLTPREFNSYGSRRGNDDIMARGTFANIRLVNHFVSAPGPRTVHFPSNEELDVFDAAERYKQEGTPLILIVGKDYGSGSSRDWAAKGPFLLGIKAVIAESYERIHRSNLVGMGVVPLQFLNGQNAKSLGLTGKEVFSIDIPSDLRPLQTVSVQTDAGKSFDVTVRFDTEVDLLYYRHGGILNYMIRKMLA 901          

BLAST of aconitase vs. nr
Match: gi|242018544|ref|XP_002429734.1| (aconitase, putative [Pediculus humanus corporis] >gi|212514746|gb|EEB16996.1| aconitase, putative [Pediculus humanus corporis])

HSP 1 Score: 1347.8 bits (3487), Expect = 0.000e+0
Identity = 645/871 (74.05%), Postives = 748/871 (85.88%), Query Frame = 0

Query:   42 KFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMING-LGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            K+FDL + G  Y +LP+SIRVLLESAVRNCDNFQV E DV  IL+W  NQ VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDFV+T DAL KNE+LEFERNKERFTFLKWGAKAF+NMLIVPPGSGIVHQVNLE+LAR+ F   +++YPDSVVGTDSHTTMING LGVVGWGVGGIEAEAVMLGQ ISM+LP+V+GYKI+G +   VTSTD+VLTITKHLRQ+GVVGKFVEF+GPGV++LSIADRATI+NMCPEYGATVG+FPVD +S+DYLK+TNR  +KIK +E YL    M+R+Y+D++QDP +S+V+ELDL +VV S+SGPKRPHDRVSVS+MKTDFLNCL+  +GFKG+GL  ++      F   GK++++ HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAV+ GL+  PYIKTSLSPGSGVVTYYLKESGV+PYL+ LGFS+VGYGCMTCIGNSGPL   V EAI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAY IAGRVDIDFETE LG   DGK ++LRDIWP+R EIQ VEKK+VIP MFEEVY KI  G+  W  ++AP  KLYPW++ STYIK PPFFDGMT+ LP  + V+NA  LLNL DSVTTDHISPAGSIARNSPAARFL SKG+ PR+FNSYGSRRGNDAVMARGTFANIRLVNKF+ Q GPKT+HIP+ E MDVFDAAE YK EN  L+ILAG+DYG GSSRDWAAKGP+L G++AV+AE+YERIHRSNLVGMGI+PLQY+ G++A+ L LT KE +T+ IP D+KP Q + V ++  +SF    R DTE+ELTYY++GGILNYMIRKM+
Sbjct:   22 KYFDLCAFGDAYQRLPYSIRVLLESAVRNCDNFQVTENDVKNILQWETNQKVPGGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKLLGGDPDKINPICPSDLVIDHSVQVDFVKTSDALQKNEDLEFERNKERFTFLKWGAKAFRNMLIVPPGSGIVHQVNLEHLARLVFNVDNLLYPDSVVGTDSHTTMINGRLGVVGWGVGGIEAEAVMLGQSISMLLPEVVGYKITGTLGPYVTSTDLVLTITKHLRQLGVVGKFVEFFGPGVSALSIADRATISNMCPEYGATVGFFPVDQESLDYLKKTNRPVSKIKVMEHYLKTTKMLRDYADSTQDPVYSQVIELDLGTVVSSVSGPKRPHDRVSVSEMKTDFLNCLTNKVGFKGFGLNAEQTKAMCSFNYEGKDYILKHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVQAGLTTLPYIKTSLSPGSGVVTYYLKESGVVPYLEQLGFSVVGYGCMTCIGNSGPLPPSVVEAIEKADLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYTIAGRVDIDFETEPLGMTPDGKEIYLRDIWPTRAEIQEVEKKHVIPVMFEEVYGKIQNGSLSWQSIQAPPSKLYPWNETSTYIKKPPFFDGMTRALPGVKSVKNARVLLNLGDSVTTDHISPAGSIARNSPAARFLASKGLMPRDFNSYGSRRGNDAVMARGTFANIRLVNKFMNQPGPKTIHIPTGEEMDVFDAAERYKMENTPLIILAGKDYGSGSSRDWAAKGPYLLGIKAVIAETYERIHRSNLVGMGIIPLQYLEGQDAEKLKLTGKETYTIDIPDDVKPLQKITVNLNDGRSFDTRLRLDTEVELTYYKNGGILNYMIRKML 892          

BLAST of aconitase vs. nr
Match: gi|998504501|ref|XP_015512413.1| (PREDICTED: cytoplasmic aconitate hydratase-like [Neodiprion lecontei])

HSP 1 Score: 1345.49 bits (3481), Expect = 0.000e+0
Identity = 653/888 (73.54%), Postives = 755/888 (85.02%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +P+  LL+ +++ G  + +++D+AS G +Y +LPFSIRVLLESAVRNCD FQV +TDV KILKW  NQ VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDF R  D+L KNEELEFERNKERF FLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARV F +  ++YPDSVVGTDSHTTMINGLGV+GWGVGGIEAEAVMLGQ ISM+LPKV+GYK+ G ++  VTSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA LSIADRATI+NMCPEYGATVG+F VD QS+ YL+QT+R    +  IE YL  V M+RNY+D SQDP FSEVV LDLA+VV S+SGPKRPHDRVSVSDMK DFL+CLSA IGFKG+GL  +KL T   F   GK++ ++HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL+VAPYIKTSLSPGSGVVTYYLKESGVIP L  LGF +VGYGCMTCIGNSGPL + + +AI+K +LVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAG V IDF+TE LG+ ADGK +FLRDIWP+R EIQ VE+K+VIP MF EVYSKI  G+  W+ L+APEGKLYPWD  STYIK PP+F+G+TKELP  R +  A  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+ P+EFNSYGSRRGNDAVMARGTFANIRLVNKFIG+AGP+T++IP+NE MD+FDAAE+Y  +   L+ L G++YG GSSRDWAAKGPFLQG+RAV+AESYERIHRSNLVGMGI+PLQY+ G+ ADSLGLT  E + + IP D +P QT+ V     K F V+ RFDTE++LTY++HGGILNYMIR M+
Sbjct:    5 NPYKRLLKTIEIGGI-DCQYYDVASFGDKYDKLPFSIRVLLESAVRNCDEFQVKKTDVEKILKWETNQKVPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKSLGGDPDKINPICPSDLVIDHSVQVDFTRGDDSLKKNEELEFERNKERFMFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVVFNNDGVLYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQAISMLLPKVVGYKLEGILNQYVTSTDLVLTITKNLRQLGVVGKFVEFFGPGVAELSIADRATISNMCPEYGATVGFFAVDQQSLSYLRQTSRSEKHVTYIEEYLRTVKMLRNYNDPSQDPVFSEVVTLDLATVVSSVSGPKRPHDRVSVSDMKNDFLSCLSAKIGFKGFGLKPEKLKTEGKFQFEGKDYTLNHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLTVAPYIKTSLSPGSGVVTYYLKESGVIPSLMQLGFDVVGYGCMTCIGNSGPLPDSMVDAIEKNELVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGTVIIDFDTEPLGRRADGKEIFLRDIWPTRAEIQTVEQKHVIPAMFTEVYSKIEKGSGSWSSLQAPEGKLYPWDAESTYIKHPPYFEGLTKELPPARPIRRARVLLNLGDSVTTDHISPAGSIARNSPAARYLAKRGLKPKEFNSYGSRRGNDAVMARGTFANIRLVNKFIGKAGPRTIYIPTNEEMDIFDAAEKYVNDGVPLIALVGKEYGSGSSRDWAAKGPFLQGIRAVIAESYERIHRSNLVGMGIIPLQYLPGQTADSLGLTGLELYDIIIPTDCRPGQTITVTTDDGKRFDVIVRFDTEVDLTYFKHGGILNYMIRTMV 891          

BLAST of aconitase vs. nr
Match: gi|665796130|ref|XP_008545838.1| (PREDICTED: cytoplasmic aconitate hydratase [Microplitis demolitor])

HSP 1 Score: 1343.56 bits (3476), Expect = 0.000e+0
Identity = 634/891 (71.16%), Postives = 745/891 (83.61%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            + P+P+ +LL+ + +       ++D++S G +Y +LPFSI++LLESAVRNCD FQV ++DV KIL W  NQ+    VEV F+PARVILQD TGVPAVVDFAAMRD V+RL GDP  INP CPSDLVIDHS+QVDFVR+ DAL KNE+LEFERNKERF FLKWGAKA KNMLIVPPGSGIVHQVNLEYLARV F   D++YPDSVVGTDSHTTMINGLG++GWGVGGIEAEAVMLGQ +SM+LPKV+GYK+ G ++  VTSTD+VLTITK+LRQ+GVVGKFVEFYGPGVA LSIADRATI+NMCPEYGATVG+FPVD QS++YL+QT R    I  IE YL AV M+R+Y+  SQDP FSE V LDLA+VV S+SGPKRPHDRVSVSDMK DF  CL+  IGFKGYGL  DK+ T   F+  G+E+ + HG+V+IAAITSCTNTSNPSVMLGAGL+AK AV+ GL+VAPYIKTSLSPGSGVVTYYL+ESGVIP L  LGF IVGYGCMTCIGNSGPLE+ ++E I+K +LVCCGVLSGNRNFEGRIH  TRANYLASP LVIAYAIAG VDIDFETE LG+ ADGK ++LRDIWP+R +IQAVE+KYVIP MF+ VY KI  G++RW  L APEG+LYPW+++STYIK PP+F+ + KELP  + +  A  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+TPREFNSYG+RRGNDAVM RGTFANIRL+NKF+G+ GPKTL++P+NE MD+FDAAE Y K+   L+ L G++YG GSSRDWAAKGP+L G+RA++AESYERIHRSNLVGMGIVPLQY+ G+ AD+LGLT  E F + IPAD +P Q + V+    K F V+ RFDTE++LTY+RHGGILNYMIR MI
Sbjct:    2 AVPNPYDNLLKSVVINSK-ERNYYDVSSFGKKYDRLPFSIKILLESAVRNCDGFQVKDSDVKKILDWEINQA-SDDVEVAFKPARVILQDLTGVPAVVDFAAMRDCVKRLNGDPNKINPICPSDLVIDHSIQVDFVRSGDALKKNEDLEFERNKERFIFLKWGAKALKNMLIVPPGSGIVHQVNLEYLARVVFDSDDLLYPDSVVGTDSHTTMINGLGILGWGVGGIEAEAVMLGQAVSMLLPKVIGYKLVGTLNQYVTSTDLVLTITKNLRQLGVVGKFVEFYGPGVAELSIADRATISNMCPEYGATVGFFPVDQQSLNYLRQTGRSEEHIDRIEKYLRAVKMLRDYNIESQDPVFSETVTLDLATVVSSVSGPKRPHDRVSVSDMKLDFNQCLTNKIGFKGYGLSADKIKTEGKFMFEGQEYTLKHGSVVIAAITSCTNTSNPSVMLGAGLLAKNAVDAGLTVAPYIKTSLSPGSGVVTYYLRESGVIPALTQLGFDIVGYGCMTCIGNSGPLEDSIAETIEKKELVCCGVLSGNRNFEGRIHPSTRANYLASPLLVIAYAIAGTVDIDFETEPLGRRADGKDIYLRDIWPTRADIQAVEQKYVIPAMFKSVYDKIEKGSNRWASLVAPEGQLYPWNEDSTYIKHPPYFETLEKELPEIKPIRKARVLLNLGDSVTTDHISPAGSIARNSPAARYLAKRGLTPREFNSYGARRGNDAVMVRGTFANIRLLNKFVGKPGPKTLYLPTNEEMDIFDAAERYGKDKVPLIALVGKEYGSGSSRDWAAKGPYLLGIRAIIAESYERIHRSNLVGMGIVPLQYLPGQTADTLGLTGVELFDIDIPADCQPHQQITVRTDTGKEFTVIVRFDTEVDLTYFRHGGILNYMIRTMI 890          

BLAST of aconitase vs. nr
Match: gi|676423145|ref|XP_009044065.1| (hypothetical protein LOTGIDRAFT_181349 [Lottia gigantea] >gi|556116868|gb|ESP05520.1| hypothetical protein LOTGIDRAFT_181349 [Lottia gigantea])

HSP 1 Score: 1338.94 bits (3464), Expect = 0.000e+0
Identity = 649/894 (72.60%), Postives = 751/894 (84.00%), Query Frame = 0

Query:   19 APSAPSPFGHLLRDLKVEGHGNLKFFDLASLGPE-YGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            A     PF  LL+    EG  + KFFDLA L  E Y +LPFSIRVLLESAVRNCD F + ++DV  IL W++ Q     VE+PF+PARVILQDFTGVPAVVDFAAMRDAV+++GGDPE INP CP+DLVIDHSVQVD  +T DAL KN+ELEF+RNKERF FLKWGAKA KNMLIVPPGSGIVHQVNLEYLARV F  +  +YPDS+VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQ ISMVLP+V+GYK+ G   S +TSTD+VLT+TKHLRQIGVVGKFVEF+GPGV SLSIADRATI+NMCPEYGAT+G+FPVD  SI YLKQT RD  K+  IE YL    M R+YS+  +DP FS++VELDL SVV   SGPKRPHD+V VSDMKTDF NCL   + FKG+ +PKDK +T VP V + KE++++HG+V+IAAITSCTNTSNPSVMLGAGL+A+ AVE GL+++PYIKTSLSPGSGVVTYYL+ESGVIPYL+ LGF +VGYGCMTCIGNSGPL EPV+E I+KGDLVC GVLSGNRNFEGRIH  TRANYLASPPLVIAYAIAG V+IDFETE LG  ADG PVFLRDIWP RE+I  VEK++V+P+MF++VYSKIT+GN RWN LEAPE  LYPWD+ STYIKSPPFF+ MTKE+  P  +ENA  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+ PREFNSYGSRRGNDAVMARGTFANIRL+NKFIG+ G +T+++P+ + MDVFDAAE Y+KEN+ ++ILAG++YG GSSRDWAAKGP++QGV+AV+AESYERIHRSNLVGMGIVP QY+ G+NA+SLGLT KE F+V +P D+K  Q L V V   KSF V  RFDTE+EL Y++HGGILNYMIR M+
Sbjct:    2 ASDGTHPFASLLKSQSFEGK-DYKFFDLAGLKDERYDKLPFSIRVLLESAVRNCDEFVIRKSDVENILNWSKTQH--EQVEIPFKPARVILQDFTGVPAVVDFAAMRDAVKKMGGDPEKINPICPADLVIDHSVQVDVSKTSDALEKNQELEFQRNKERFLFLKWGAKALKNMLIVPPGSGIVHQVNLEYLARVVFDSNGTLYPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQSISMVLPEVIGYKLCGTPGSYITSTDIVLTVTKHLRQIGVVGKFVEFFGPGVNSLSIADRATISNMCPEYGATIGFFPVDDASISYLKQTGRDDEKLILIEKYLQLNKMFRDYSNPEEDPVFSQIVELDLGSVVSCCSGPKRPHDKVPVSDMKTDFQNCLDNKVSFKGFAIPKDKQSTNVPIVYDNKEYILNHGSVVIAAITSCTNTSNPSVMLGAGLLARNAVEAGLTISPYIKTSLSPGSGVVTYYLRESGVIPYLEKLGFDVVGYGCMTCIGNSGPLPEPVAEGIEKGDLVCVGVLSGNRNFEGRIHPLTRANYLASPPLVIAYAIAGTVNIDFETEPLGTRADGTPVFLRDIWPKREDIVTVEKQFVVPSMFKDVYSKITSGNKRWNALEAPESMLYPWDEKSTYIKSPPFFEKMTKEIQPPSSIENAYVLLNLGDSVTTDHISPAGSIARNSPAARYLAGRGLAPREFNSYGSRRGNDAVMARGTFANIRLLNKFIGKPGARTVYVPTGQEMDVFDAAERYQKENKQVIILAGKEYGSGSSRDWAAKGPWMQGVKAVIAESYERIHRSNLVGMGIVPFQYLDGQNAESLGLTGKETFSVSVPEDVKAGQILTVNVKDGKSFDVKTRFDTEVELAYFKHGGILNYMIRGML 892