triple functional domain partial, maker-scaffold462_size163801-snap-gene-0.52 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of triple functional domain partial vs. L. salmonis genes
Match: EMLSAG00000002417 (supercontig:LSalAtl2s:LSalAtl2s145:455239:514648:-1 gene:EMLSAG00000002417 transcript:EMLSAT00000002417 description:"augustus_masked-LSalAtl2s145-processed-gene-5.0") HSP 1 Score: 1264.6 bits (3271), Expect = 0.000e+0 Identity = 655/988 (66.30%), Postives = 763/988 (77.23%), Query Frame = 0
Query: 311 FSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM--ELDASGS----------MNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSD-----------------------------SQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGR-STGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
S+CEGG+SYDSGYSGRDS+A NM CNPDMQA IP IMQ+LD VHSGQQQLLQLWQIKK KLDQC QLRLFEQDCEKMFDWIHH+R FL NYV IG S+ +KK+Q+DH+ FT+ +NNVF +++ IL++A+KLIE+NHYASQHVR VA RLDK WK+FA +G + +TDI LE+SIH HQ+LYE MCQ+YTEVHSTSKKLLYQLDHLVQ + ++ +KH + + G P D DYSEGASHVL+VIH IL HHR+LE KWH KKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRS+ KARAYQKSHETFEGVVQNTYTNAEKLLAAA+ELAQTGECNA+DIYSVAHELESHIS FAQRVE RR++LELA FY+H EEL WLS+L+QEL SE+VS+SLE +ERLIEQFSNQRDSTLDA A+TI EGKTL+EE+K N + ++++SG + +V + LD+L Q++EL +W+TRKMRLDLC QLRLFERDALEL SQYELWA+QL NGD+PR++KETE +LRNL++H+ HIQ AT+EVAQRGQ+LL+ E+SGLT+M+D ERE+D+E+ S RR+KLEQ IQL QFE DANQVIRWI+SAEGML AGFS+PG L DAEQ+KKEHEQFQTAIEKTHASAVHVRQRAE LL NNH++P VK+IAD VT+RWQ LVT+AEERHKLVTASLNFYKTAEQVCSVLDSLE+EY+RDDDWL R ST +S+ D+V + Q INKHQEQKEAFLKACTLARRTAETF+KYSNRSLQYF + EA+F GPE+KVK ILE+LL+QENRVLEYWTTKKKRL+Q+QQFCLFERSA+QSL WIKEEGDIYL++H + VGTTKEET++LL EHNSFK A+
Sbjct: 162 LSSCEGGSSYDSGYSGRDSSAXNMACNPDMQACIPQIMQLLDQVHSGQQQLLQLWQIKKVKLDQCVQLRLFEQDCEKMFDWIHHNREAFLSNYVEIGHSYSLAKKIQNDHDQFTIAANNVFVDVSGILTEASKLIENNHYASQHVRNVATRLDKTWKEFA-------------------------------EG-------AVSTDIPVLEASIHHHQTLYENMCQAYTEVHSTSKKLLYQLDHLVQICN-VKEDVQRKHSAMNKG--PSNVDPTADYSEGASHVLSVIHEILGHHRSLEVKWHAKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSIAKARAYQKSHETFEGVVQNTYTNAEKLLAAASELAQTGECNADDIYSVAHELESHISGFAQRVEVRRRILELAAMFYAHVEELNGWLSDLRQELMSEDVSDSLEESERLIEQFSNQRDSTLDAIANTITEGKTLIEEVKINAMNRGDKVNSSGGNIVSEVESSSVVAVNAALDKLNVQKEELNNLWSTRKMRLDLCLQLRLFERDALELLSQYELWADQLNNGDIPRELKETEIKLRNLTDHVNHIQXATFEVAQRGQDLLQRFESSGLTIMADVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEREMDIEDFSSIRRVKLEQRIQLCQFECDANQVIRWIQSAEGMLTAGFSVPGSLIDAEQLKKEHEQFQTAIEKTHASAVHVRQRAETLLNNNHYDPQSVKDIADRVTSRWQQLVTQAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKRDDDWLSRHSTPSSE--KDRVTFILQGINKHQEQKEAFLKACTLARRTAETFIKYSNRSLQYF--SHNPEATFAGPESKVKA----------ILEKLLSQENRVLEYWTTKKKRLDQHQQFCLFERSARQSLEWIKEEGDIYLNTH-TNVGTTKEETQSLLQEHNSFKEKAK 1093 HSP 2 Score: 96.2857 bits (238), Expect = 4.933e-20 Identity = 81/374 (21.66%), Postives = 165/374 (44.12%), Query Frame = 0
Query: 367 IKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTK-WSQGCDMTSAMSAATD----ISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIA 732
I++ KL+Q QL FE D ++ WI + S+ S ++++L+ +H F ++ + A L+ +NHY Q V+ +A R+ W+ +ER ++ S+ F++ A+Q +DS+ + + + D S S + ++ + I++HQ E ++ T T++ + + +Q S H + P K V A++ +L + W KK +L Q LF+ +Q L+W++ G+++L + +G + ++ ++ + H +F+ + T + LL A L + G +A I + E++ F++R++E R LE ++
Sbjct: 799 IRRVKLEQRIQLCQFECDANQVIRWIQSAEGMLTAGF-SVPGSLIDAEQLKKEHEQFQTAIEKTHASAVHVRQRAETLLNNNHYDPQSVKDIADRVTSRWQQLVTQAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKRDDDWLSRHSTPSSEKDRVTFILQGINKHQEQKEAFLKACTLARRTAETFIKYSNRSLQYFS---------HNPEATFAGPESK------------VKAILEKLLSQENRVLEYWTTKKKRLDQHQQFCLFERSARQSLEWIKEEGDIYLNTHTNVGTTKEETQSLLQEHNSFKEKAKETREKVKLLLQLADTLVEKGHAHAPTIKNWVTEVDETYKDFSRRMDEYRVKLEKSLG 1150 HSP 3 Score: 87.8113 bits (216), Expect = 1.962e-17 Identity = 84/362 (23.20%), Postives = 161/362 (44.48%), Query Frame = 0
Query: 615 KKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEE---------------VSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLE----ELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCI 957
+++KL QR+ L F+ D QV+ W+Q+ E L + SL A +K HE F+ ++ T+ +A + A L + + + +A + S + EER KL+ ++ FY E++ S L L++E + ++ V+ L+G I + Q+++ L AC +T ++ L+ E +G + V++ L++L +Q + + E W T+K RLD Q LFER A Q W + + GD+ + + ++ + ++ E A+ +E +K+L TL+ + + ++ + + E D + R+KLE+ +
Sbjct: 800 RRVKLEQRIQLCQFECDANQVIRWIQS-AEGMLTAGFSVPGSLIDAEQLKKEHEQFQTAIEKTHASAVHVRQRAETLLNNNHYDPQSVKDIADRVTSRWQQLVTQAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKRDDDWLSRHSTPSSEKDRVTFILQG----INKHQEQKEAFLKACTLARRTAETFIKYSNRSLQYFSHNPEATFAGPESKVKAILEKLLSQENRVLEYWTTKKKRLDQHQQFCLFERSA----RQSLEWIK--EEGDIYLNTHTNVGTTKEETQSLLQEHNSFKEKAKETREKVKLLLQLADTLVEKGHAHAPT-IKNWVTEVDETYKDFSRRMDEYRVKLEKSL 1149 HSP 4 Score: 67.781 bits (164), Expect = 2.388e-11 Identity = 36/141 (25.53%), Postives = 72/141 (51.06%), Query Frame = 0
Query: 337 NPDMQASIP--IIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDE 475
NP+ + P + +L+ + S + ++L+ W KK +LDQ Q LFE+ + +WI +L+ + ++G + +E++ L +HN F + + +L A L+E H + ++ +D+ +KDF+ +DE
Sbjct: 1000 NPEATFAGPESKVKAILEKLLSQENRVLEYWTTKKKRLDQHQQFCLFERSARQSLEWIKEEGDIYLNTHTNVGTTKEETQSLLQEHNSFKEKAKETREKVKLLLQLADTLVEKGHAHAPTIKNWVTEVDETYKDFSRRMDE 1140
BLAST of triple functional domain partial vs. L. salmonis genes
Match: EMLSAG00000007876 (supercontig:LSalAtl2s:LSalAtl2s462:46517:122067:-1 gene:EMLSAG00000007876 transcript:EMLSAT00000007876 description:"maker-LSalAtl2s462-augustus-gene-1.20") HSP 1 Score: 90.5077 bits (223), Expect = 2.838e-18 Identity = 197/957 (20.59%), Postives = 389/957 (40.65%), Query Frame = 0
Query: 340 MQASIPI----IMQMLDTVHSGQQQLLQLW-------QIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILS-DAA--KLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAE---LAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAI---AFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQR---DSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQ----LQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAA---GFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERH-KLVTASL--NFYKTAEQVCSVLDSLEKEYRRDDDWLGRSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQG--SEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSARLFMRT 1261
M+ +P+ I++ + + ++Q+L+ + ++K+ KL+ + + F +D E++ WI+ + Q + ++++ LQ V ++ N I++ D A ++I +H+ S +R L + W L E+ L +++ Q +Q D V W D + ++A LE E + + + E Y++ + T+ + EH + E+ V K++ + A L + N G+K KL ++ F D + + W+ V + G G L +A Q+ HE GV ++ +K+L E L Q +AE I + E+ + + ++RR+ L+ + F + ++L SW+++++ ++++E+++ + GAE L+E + + D D+ +T G+ LL + + VQ L L ++++L E+W R++ + C L+LF RD + Q + W + L N DL + E+ ++ H AQ +E +K L+ L+ Y Q ++ R + LE+ +QR+ L + QF+ DA++ WI E + A + P L Q +H+ F+ + + + E L++++H ++K + VT W+GL ++ KL AS + + E + L +E + +D G+ + + K AL+ ++ HQ++ + R ++ F K + Q E F E K + L L N++ + +K+ LE + Q + A ++ +W+KE+ + + G ++ +EALL H +F S F T
Sbjct: 820 MEQPVPVKEVKILETAEDIQERREQVLRRYVEFKDAARVKREKLEDSRRFQYFRRDAEELESWIYE-KLQ-----AASDENYKDPTNLQAKIQKHQAFEAEVAAHSNAIVTLDNAGMEMITQDHFQSMEIREKLDELHRLWDLLLSKLAEKGMRLQQALVLVQFLRQ-SDEVMFWI--TDKEAFVTADEYGHDLEH--------VEVLQRKFEEFQKDMASQEYRVSEVGDTADKLVGDEHPESET----VIKKKEEV-----QAAWARLKKLAN-------------GRKEKLFGAHEIQRFNRDADETIAWITEKDAVLSSDDYGKG--LASVQALQRKHE---GVERDLAALEDKVLTLGQEADRLCQIHPEHAEQIKAKHQEISKNWEQLLDKAKDRRQKLDESYHLHRFLADYKDLTSWINDMQAVIEADELAKDVNGAEALLESHAEHKSEIDVREDSFRATAEAGQMLLNS-----------GHSASDEVQEKLINLANEKNKLLELWEERRVLYEQCMDLQLFYRD----TEQTDTWMGKQEAFLTNKDLGDSLDSVEAMIKK------HEDFEKSLAAQ--EEKIKALDEFATKLIKGQHYAADEVAQKRALLLERRALLLEK-SAQRKAMLHDSLCFQQFDRDADETKGWIN--EKLKVATDDSYLDPTNLNGKVQ---KHQNFEVELNANKSRFEDLINTGEKLIESDHVMSSQIKAKLEEVTNLWEGLCNSTNKKSIKLEEASQQQTYNRGIEDLELWLSEVEGQLGSED--YGKDLTSVQNLQKKHALLEADVSSHQDRVDG-------VRIASDQFAKSGHFDADNIVTAQRMVEEGHFASDEIKTR------------LGLLHDHWNQLKDKAAQRKQDLEDSLQAHQYFADANEAESWMKEKEPL---AGNEEYGRDEDGSEALLKRHEAFMSDLEAFGNT 1679 HSP 2 Score: 78.5666 bits (192), Expect = 1.277e-14 Identity = 120/504 (23.81%), Postives = 219/504 (43.45%), Query Frame = 0
Query: 628 FQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERR-KLLELAIA--FYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEEL--KSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYN----GQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRW-IKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHF--NPGRVKEIADGVTTRWQGLVTRA-EERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG--RSTGNSDHHHDKVALVAQLINKHQ 1116
F DV++ DW+Q E + +G+ L+ +A Q+ HE E + +L A +L T +AE I E+ + + +R+ KLL+ F S +L+SW++ +K + ++E++ + GAE L+E+ R + +DA + T + ++L SN ++E VQ L+ + A R++L + W R+M+LD C +L+LF RD Q E W +Q+ + E +++ N+ I + + + +E ++ L L++ Y G + +VL R +L+E ++R KL + L QF DA+++ W ++ + + P +Q Q +H+ F+ + V + L+ + V+ ++ +W+ L + E+ KL A N +T + +V D D WLG S N+D +A V L+ KHQ
Sbjct: 1852 FHRDVEETKDWIQEKDEALSIDD--LGKDLRSVQALQRKHEGLERDLAALGDKIRRLDEGATKLMTTHSESAEVIQEKKKEIYEEWTQLIAKAGKRKDKLLDSYDLQRFLSDYRDLMSWINSMKSLIANDELASDVTGAEALLERHQEHR-TEIDARSGTFQAFELFGQQLLHGSNYASIE---------VQEKLESMSAAREDLEKAWIARRMQLDECLELQLFYRDC----EQAENW---MQSRETFLASDEVDNEGDNVEALIKKHEDFDKAIGNQ-EEKIESLSTYADQLIASDHYAKGDVGDKKKEVL-----SRWEELKEALIEKRSKLGESQTLQQFSRDADEIENWMLEKLQLAEEENYKDPANIQSKHQ---KHQAFEAELGANSDRIQSVLSMGQNLIDRRKCAGSEDAVQNRLRSISEQWEILTQKTCEKSMKLKEA--NRQRTF--IAAVKDL--------DFWLGEVESLLNTDDSGKDLASVQNLMKKHQ 2315 HSP 3 Score: 65.855 bits (159), Expect = 8.791e-11 Identity = 133/703 (18.92%), Postives = 278/703 (39.54%), Query Frame = 0
Query: 372 LDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQR-VEERRKLLELAI--AFYSHTEELISWLSELKQELQSEEVSE--SLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELW---AEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNF 1066
++ H+++ F +D E+ DWI + +G+ + + LQ H + I R+ A KL+ ++ +++ ++ + + W +R L S + Y D ++ + + + A+D++ E+ + +HQ + TE+ + S +Q L G+ H S ++ + +K + ++ LE W ++++L + L L+LF D +Q +W+Q+ E FL + + A K HE F+ + N E L A +L + D+ E+ S + +E+R KL E F +E+ +W+ E Q + E + +++ + + F + + D S ++ G+ L++ K +GS ++VQ+ L + Q + L + + M+L ++ R F + + W E L N D D + + ++NL + +++ R +K + +L+S Q+ + +Q I +R + +L + R+ L + L QF D WIK + +L + L + +KK+H++F++ + + V+ E L+ + +K+ + W+ L A R + + S+ +
Sbjct: 1843 FERAHEVQRFHRDVEETKDWIQEKDEAL--SIDDLGKDLRSVQALQRKHEGLERDLAALGDKIRRLDEGATKLMTTHSESAEVIQEKKKEIYEEWTQLIAKAGKRKDKLLDSYDLQRFLSDYRDLMSWINSMKSLIANDELASDVTGAEALLERHQ-------EHRTEIDARSGT--FQAFELF--------GQQLLHGSNYASIEVQEK----------------LESMSAAREDLEKAWIARRMQLDECLELQLFYRDCEQAENWMQSR-ETFLASD-EVDNEGDNVEALIKKHEDFDKAIGNQEEKIESLSTYADQLIASDHYAKGDVGDKKKEVLSRWEELKEALIEKRSKLGESQTLQQFSRDADEIENWMLEKLQLAEEENYKDPANIQSKHQKHQAFEAELGANSDRIQSVLSMGQNLIDRRK---------CAGSEDAVQNRLRSISEQWEILTQKTCEKSMKLKEANRQRTF----IAAVKDLDFWLGEVESLLNTD---DSGKDLASVQNLMKKHQLVEADILAHEDR----IKDMNEQADSLISSGQFEAK-EIQDKRSSINDRYKHILDLAAHRQSLLNEASTLHQFFRDIADEESWIKEKK-LLVSSDDYGRDLIGVQNLKKKHKRFESELASHEPTIKSVQDAGETLIGVSQMGETEIKQRLRQLNDLWEELKEMASTRGRKLDESITY 2486 HSP 4 Score: 58.151 bits (139), Expect = 1.975e-8 Identity = 124/601 (20.63%), Postives = 233/601 (38.77%), Query Frame = 0
Query: 245 LEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKV--LKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEV-FLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIA---FYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLD 839
L+ F+ ++M ++ M+ + +++ A DV GA E H E + ++ + + GQ LL G++Y ASI + + L+++ + ++ L + W ++ +LD+C +L+LF +DCE+ +W+ R FL + + + L H F N I + + A +LI S+HYA V + W++ L E+ + LG S Q ++ D + W + D + ++S +HQ+ E+ + S ++ S + G++ + +G SE A V + +I + L K K +KL + R F VK + WL GEV L G+ L + K H+ E + + + A L +G+ A++I + R+ LL A F+ + SW+ E K + S++ L G + L ++ S +A + ++ ++ G T+ + ++ L +L +EL E+ +TR +LD
Sbjct: 1955 LQRFLSDYRDLMSWINSMKSLIANDELASDVTGAEALLERHQEHRTEIDARSGTFQAFELFGQQLLH-------GSNY---------------------ASIEV-QEKLESMSAAREDLEKAWIARRMQLDECLELQLFYRDCEQAENWM-QSRETFLASD-EVDNEGDNVEALIKKHEDFDKAIGNQEEKIESLSTYADQLIASDHYAKGDVGDKKKEVLSRWEELKEALIEKRSKLGESQTLQQFSRD-ADEIENWMLEKLQLAEEENYKDPANIQSKHQKHQAF-------EAELGANSDRI----------QSVLSMGQNLIDRRKCAG------------SEDA--VQNRLRSISEQWEILTQKTCEKSMKLKEANRQRTFIAAVKDLDFWL---GEVESLLNTDDSGKDLASVQNLMKKHQLVEADILAHEDRIKDMNEQADSLISSGQFEAKEIQDKRSSINDRYKHILDLAAHRQSLLNEASTLHQFFRDIADEESWIKEKKLLVSSDDYGRDLIGVQNLKKKHKR--------FESELASHEPTIKSVQDAGETLIGVSQMGETEIKQRLRQLNDLWEELKEMASTRGRKLD 2481 HSP 5 Score: 53.5286 bits (127), Expect = 4.722e-7 Identity = 116/573 (20.24%), Postives = 218/573 (38.05%), Query Frame = 0
Query: 248 FMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRK--VLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESS-----IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQN-HGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELE-----------------SHISSFAQRVEERRKLLELAI---AFYSHTEELISWLSELKQELQSEEVSESLEGAERLI---EQFSNQRDSTLDACASTIAEGK 789
F+ ++ ++ M+ + ++ A DVNGA E H E K + V + GQ+LL +SG+S D + + L + + + +LL+LW+ ++ +QC L+LF +D E+ W+ FL N +G S + + H F I + A KLI+ HYA+ V A L + +R +L S+ F Q+ + D W + + ATD S L+ + + +HQ+ EV + K + + L+ T + +H +S + A + + + L + K IKL + + + ++ + WL G++ + G+ L + QK H E V + + + A+ + A++G +A++I + +E H + + +R++ LE ++ +++ E SW+ E + +EE +G+E L+ E F S L+A +TI + K
Sbjct: 1189 FLADYKDLTSWINDMQAVIEADELAKDVNGAEALLESHAEHKSEIDVREDSFRATAEAGQMLL------------NSGHSASDE-----------------VQEKLINLANEKNKLLELWEERRVLYEQCMDLQLFYRDTEQTDTWMGKQEA-FLTNK-DLGDSLDSVEAMIKKHEDFEKSLAAQEEKIKALDEFATKLIKGQHYAADEVAQKRALLLERRALLLEKSAQRKAMLHDSLCF-QQFDRDADETKGW-----INEKLKVATDDSYLDPTNLNGKVQKHQNF---------EVELNANK--SRFEDLINTGEKLIESDHVM----------------------SSQIKAKLEEVTNLWEGLCNSTNKKSIKLEEASQQQTYNRGIEDLELWLSEVEGQL---GSEDYGKDLTSVQNLQKKHALLEADVSSHQDRVDGVRIASDQFAKSGHFDADNIVTAQRMVEEGHFASDEIKTRLGLLHDHWNQLKDKAAQRKQDLEDSLQAHQYFADANEAESWMKEKEPLAGNEEYGRDEDGSEALLKRHEAFM----SDLEAFGNTIQDLK 1684
BLAST of triple functional domain partial vs. L. salmonis genes
Match: EMLSAG00000012718 (supercontig:LSalAtl2s:LSalAtl2s965:459122:472828:1 gene:EMLSAG00000012718 transcript:EMLSAT00000012718 description:"maker-LSalAtl2s965-augustus-gene-3.36") HSP 1 Score: 58.9214 bits (141), Expect = 1.033e-8 Identity = 143/735 (19.46%), Postives = 287/735 (39.05%), Query Frame = 0
Query: 323 GYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHH-----RCQFLHNYVSIGRSFQESKKLQDD-HNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELA---IAFYSHTEELISWLSELKQEL-QSEEVSESLEGAERLIEQFSN-QRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNG-QSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTR 1045
G+ ++ C+P II++ + + +L+++ ++S+L++ L F D +WI + H+ +I + K ++ + H+H T +++ + N L+E H+ +R + + W + +R L +V FHQ D V W D+ +S+ D ES++ +T+ + E+ K +D L +S + GE +G + V + +I + ++ L+ +K +L L+L + V W+ ++ + + G+ ++ + + FE + + + A +L N+EDI ++L S + ++ E++R+ L A ++ E ++W+ + K+ L Q++E+ L G L + S +RD +A +T L+ L+ ++ V+ +L +EL ++ R +L+ L F +D S + N D P + E E L + I+S T + A K+ + + D QY + R++ L + +E + ++ R+ L Q + L F DA Q + E +L+ P L+ AE + K+HE T +E V Q A+ L+ HF ++++ AD ++ R
Sbjct: 591 GFVEEETPDGYKPCDP------SIIIERVQALEDAYSELVRIAVERRSRLEESKLLWQFYWDMAXEENWIKEXENILSQGDIGHDLTTINLLLSKHKSIETEIHSHETALQSSLEAGNN--------LMERGHFGEDKIRERSVEVQGMWNSLIDLMTDRKKRLTEAVDFHQFLTD-ADDVDTWM--LDILRLVSS-DDTGKDESNV-------QTLLKKQKEITDELKSYQSTIDTLHSQASQL----------------------GETDRDGPA-VKERLESIDNRYKELQELARLRKQRLLDALSLYKLCTEADGVEQWITEKEKMLV--TMTPGKDIEDCEIMKHRFDGFEREMNANASRVAVVNQLARQLLHVDHPNSEDITDRQNQLNSRWADLREKAEQKREELGSAHGVQTYHIECRETVTWIEDKKRVLEQTDELKMDLTGIMTLQRKLSGMERD---------LAAIETKLKSLEEEAEKIKEIHPEEAEVVRERNAKLRNVWEELNQMLKVRDAKLEEAGDLHRFLKDLDHFQSWLTKTESSIANEDTPSSLAEAEKLLTQHQQIHEEIESYTNDYASMMAYGEKVTADP--STFDDPQYMFLRERLKALKDGWEE----VNQMWENRQALLSQSLNLQLFNRDAKQAEVLLSQQEHLLSKD-ETPANLEQAESLIKKHEALLTTMEANDDKMNGVLQFAQRLVSEEHFGSDKIQKKADDISER 1259
BLAST of triple functional domain partial vs. SwissProt
Match: gi|257050981|sp|O75962.2|TRIO_HUMAN (RecName: Full=Triple functional domain protein; AltName: Full=PTPRF-interacting protein) HSP 1 Score: 1026.93 bits (2654), Expect = 0.000e+0 Identity = 531/1189 (44.66%), Postives = 780/1189 (65.60%), Query Frame = 0
Query: 78 GHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-ELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL--QNGDL-PRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKI---LENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG---RSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G + D ++A+DV+ +L+E++A LSGGRD+RGG I+TF A +R R ED + L+ YL +P E+V GF VI+DMRGS W ++KP+LK+LQE F IH IIKP+NFWQKQRT+ GS K++FETN +SL+ L KV+DPS LT + DG L Y+H WIE+R A ED++ +T M+ RL+ +++ L + D+ GAR E H+++K+KV+K P+E++D GQ LLQR + E +SG SG N D+Q +P + MLD +HS +Q L Q+W ++K KLDQC QLRLFEQD EKMFDWI H++ FL++Y IG S + +LQ HNHF M NV+ NINRI+S A +L+ES HYASQ +R +A++L++ WK FA LDER+T+L +S +FHQKA++Y+ +V W + C + +++ LE +IH HQ +YE + +Y+EV K LL +L + S+ + +YS+ HVL VIH +L H R LE W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK HE FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY AH+LE I F +RVE+R+ LL+++++F++H +EL +WL EL++EL + +ES+E + LI++F Q+ +TL + I EG+ L+++L+ + I+ + + S+N +++ L +L + ++ E++ RK++L+L QLR+FERDA+++ S E W ++L Q D D+ E +L++ ++ + + T++V +GQ+LL+ ++ SG+ L+ D + +RVQ LLE++ E++ +L+ Q R LEQC+QL + + QV+ WI++ E ML AG LQ+AEQ+++EHEQFQ AIEKTH SA+ V+Q+AEA+LQ NH++ +++ A+ V + WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EY+R++DW G + NS+ H V +I+KH EQKEAFLKACTLARR A+ FLKY +R+ G K PE +VK IL L +ENRVL YWT +K+RL+Q QQ+ +FERSAKQ+L WI + G+ YLS+H S G++ + T+ LL EH F+ +A+
Sbjct: 53 GFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSG-SG----------NADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKAC---GEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSS------------------DSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDH-----VTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMP---GMVTHIKAPEQQVKN----------ILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTS-TGSSIQHTQELLKEHEEFQITAK 1190 HSP 2 Score: 115.931 bits (289), Expect = 1.351e-24 Identity = 160/764 (20.94%), Postives = 326/764 (42.67%), Query Frame = 0
Query: 330 TASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKL-LYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVL-------DWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGE---CN--------AEDIYSVAHELESHISSFAQRVEERRKLLELAIAF---YSHTEELISWLSELKQELQSEEVS-ESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTR----KMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHI----AHIQSATYEVAQRGQEL-LKILENSGLTL--MSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWI-KSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEE 1055
++ ++T + + ++ ++ ++ V Q+QL +WQ +K +L Q QL +F+QD +++ DWI +H FL + +G+S ++ LQ H F + N ++N +++L A +L ++ + + A +L+ +DF +++R +L +SV FH +V + W + Q + L + +S V K+ Q L T + I+ GE + R S + K ++ +H+ V+ + + +E + +KIKL L LR+F+ D ++ D L F +++ I Q+ + + T + + + LL E+ +G C+ +D+ HE + + A E+ RK LE + + ++++ W+ + L + ++ SL+ AE+L EQF + + T + + + +L+ +N M++ V S +L + ++ ++ N K +C L E+ +Y+ + D ET+ +S+H+ A +++ T +A+R ++ LK L + + + M + +V+ +L + +RE + + R+ +L+QC Q FE A Q + WI + E L+ S +Q +++ KEHE+FQ ++T + Q A+ + H + +K+ V R++ R E+
Sbjct: 520 SSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHT----HVKELWTWLEEL--------------------QKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTP----HNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAE--QRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAA---EQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQ---ANHYDMDM-IRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQ-------EYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACT--LARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEK 1237 HSP 3 Score: 93.5893 bits (231), Expect = 9.356e-18 Identity = 84/434 (19.35%), Postives = 175/434 (40.32%), Query Frame = 0
Query: 319 SYDSGYSGRD---------STASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTK----------WSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
++D + G+D ++ + C+ D+ + + +L+ +H QQ+L + + L+QC QLR + + +++ WI + L+ + S QE+++LQ +H F + ++ A ++++NHY +R A ++ +W+ +++R ++ SV F++ ++Q + W G D S ++ + I +H E ++ T + L L +N + H P V +++ + + W +K +L Q +F+ KQ L+W+ ++GE +L + G S+Q + K HE F+ + T + L+ A + G +A +I ++ F+ R+E+ R LE A+
Sbjct: 842 TFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRV-QDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGE-SMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPM---ISKHLEQKEAFLKACTLARRNADVFLKYLH---------RNSVNMPGMVTHIKAP-------------EQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGI 1248
BLAST of triple functional domain partial vs. SwissProt
Match: gi|1133968692|sp|F1M0Z1.4|TRIO_RAT (RecName: Full=Triple functional domain protein; AltName: Full=PTPRF-interacting protein) HSP 1 Score: 1019.99 bits (2636), Expect = 0.000e+0 Identity = 528/1189 (44.41%), Postives = 778/1189 (65.43%), Query Frame = 0
Query: 78 GHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-ELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL--QNGDL-PRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKI---LENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG---RSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G + D ++A+DV+ +L+E++A LSGGRD+RGG I+TF A +R R ED + L+ YL +P E+V GF VI+DMRGS W ++KP+LK+LQE F IH IIKP+NFWQKQRT+ GS K++FETN +SL+ L KV+DPS LT + DG L Y+H WIE+R A ED++ + M+ RL+ +++ L + D+ GAR + H+++K+KV+K P+E++D GQ LLQR + E +SG SG N D+Q +P + MLD +HS +Q L Q+W ++K KLDQC QLRLFEQD EKMFDWI H++ FL++Y IG S + +LQ HNHF M NV+ NINRI+S A +L+ES HYASQ ++ +A +L++ WK FA LDER+T+L +S +FHQKA++Y+ +V W + C + +++ LE +IH HQ +YE + +Y+EV K LL +L + S+ + +YS+ HVL VIH +L H R LE W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK HE FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY AH+LE I F +RVE+R+ LL+++++F++H +EL +WL EL++EL + +ES+E + LI++F Q+ +TL + I EG+ L+++L+ + I+ + + S+N +++ L +L + ++ E++ RK++L+L QLR+FERDA+++ S E W ++L Q D D+ E +L++ ++ + + T++V +GQ+LL+ ++ SG+ L+ D + +RVQ LLE++ E++ +L+ Q R LEQC+QL + + QV+ WI++ E ML AG LQ+AEQ+++EHEQFQ AIEKTH SA+ V+Q+AEA+LQ NH++ +++ A+ V + WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EY+R++DW G + NS+ H V +I+KH EQKEAFLKACTLARR A+ FLKY +R+ G K PE +VK IL L +ENRVL YWT +K+RL+Q QQ+ +FERSAKQ+L WI + G+ YLS+H S G++ + T+ LL EH F+ +A+
Sbjct: 53 GFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRMRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHIALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNAAHMLSRLEELQDVLAKKELPQDLEGARNMIDEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSG-SG----------NADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGASHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKAC---GEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSS------------------DSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDH-----VTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRN---SVSMPGMVTHIKAPEQQVKN----------ILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTS-TGSSIQHTQELLKEHEEFQITAK 1190 HSP 2 Score: 115.931 bits (289), Expect = 1.328e-24 Identity = 160/764 (20.94%), Postives = 327/764 (42.80%), Query Frame = 0
Query: 330 TASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKL-LYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVL-------DWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGE---CN--------AEDIYSVAHELESHISSFAQRVEERRKLLELAIAF---YSHTEELISWLSELKQELQSEEVS-ESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTR----KMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHI----AHIQSATYEVAQRGQEL-LKILENSGLTL--MSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWI-KSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEE 1055
++ ++T + + ++ ++ ++ V Q+QL +WQ +K +L Q QL +F+QD +++ DWI +H FL + +G+S ++ LQ H F + N ++N +++L A +L ++ + + A +L+ +DF +++R +L +SV FH +V + W + Q + L + +S V K+ Q L T + I+ GE + R S + K ++ +H+ V+ + + +E + +KIKL L LR+F+ D ++ D L F +++ I Q+ + + T + + + LL E+ +G C+ +D+ HE + + A E+ RK LE + + ++++ W+ + L + ++ SL+ AE+L EQF + + T + + + +L+ +N M++ V S +L + ++ ++ N K +C L E+ +Y+ + D ET+ +S+H+ A +++ T +A+R ++ LK L + +++ M + +V+ +L + +RE + + R+ +L+QC Q FE A Q + WI + E L+ S +Q +++ KEHE+FQ ++T + Q A+ + H + +K+ V R++ R E+
Sbjct: 520 SSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHT----HVKELWTWLEEL--------------------QKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTP----HNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAE--QRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAA---EQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQ---ANHYDMDM-IRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQ-------EYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACT--LARRNADVFLKYLHRNSVSMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEK 1237 HSP 3 Score: 92.4337 bits (228), Expect = 1.822e-17 Identity = 89/441 (20.18%), Postives = 179/441 (40.59%), Query Frame = 0
Query: 319 SYDSGYSGRD---------STASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTK----------WSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAV---IHNILDHHRTLETK----WHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
++D + G+D ++ + C+ D+ + + +L+ +H QQ+L + + L+QC QLR + + +++ WI + L+ + S QE+++LQ +H F + ++ A ++++NHY +R A ++ +W+ +++R ++ SV F++ ++Q + W G D S ++ + I +H E ++ T + L L H+ S V +H+ A + NIL+ E + W +K +L Q +F+ KQ L+W+ ++GE +L + G S+Q + K HE F+ + T + L+ A + G +A +I ++ F+ R+E+ R LE A+
Sbjct: 842 TFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRV-QDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGE-SMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPM---ISKHLEQKEAFLKACTLARRNADVFLKYL--------------HRNSVSMPGMV---------------THIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGI 1248
BLAST of triple functional domain partial vs. SwissProt
Match: gi|257051075|sp|Q0KL02.3|TRIO_MOUSE (RecName: Full=Triple functional domain protein) HSP 1 Score: 1016.91 bits (2628), Expect = 0.000e+0 Identity = 526/1189 (44.24%), Postives = 778/1189 (65.43%), Query Frame = 0
Query: 78 GHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-ELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL--QNGDL-PRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKI---LENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG---RSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G + D ++A+DV+ +L+E++A LSGGRD+RGG I+TF A +R R ED + L+ YL +P E+V GF VI+DMRGS W ++KP+LK+LQE F IH IIKP+NFWQKQRT+ GS K++FETN +SL+ L KV+DPS LT + DG L Y+H WIE+R A E+++ + M+ RL+ +++ L + D+ GAR + H+++K+KV+K P+E++D GQ LLQR + + +SG SG N D+Q +P + MLD +HS +Q L Q+W ++K KLDQC QLRLFEQD EKMFDWI H++ FL++Y IG S + +LQ HNHF M NV+ NINRI+S A +L+ES HYASQ ++ +A +L++ WK FA LDER+T+L +S +FHQKA++Y+ +V W + C + +++ LE +IH HQ +YE + +Y+EV K LL +L + S+ + +YS+ HVL VIH +L H R LE W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK HE FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY AH+LE I F +RVE+R+ LL+++++F++H +EL +WL EL++EL + +ES+E + LI++F Q+ +TL + I EG+ L+++L+ + I+ + + S+N +++ L +L + ++ E++ RK++L+L QLR+FERDA+++ S E W ++L Q D D+ E +L++ ++ + + T++V +GQ+LL+ ++ SG+ L+ D + +RVQ LLE++ E++ +L+ Q R LEQC+QL + + QV+ WI++ E ML AG LQ+AEQ+++EHEQFQ AIEKTH SA+ V+Q+AEA+LQ NH++ +++ A+ V + WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EY+R++DW G + NS+ H V +I+KH EQKEAFLKACTLARR A+ FLKY +R+ G K PE +VK IL L +ENRVL YWT +K+RL+Q QQ+ +FERSAKQ+L WI + G+ YLS+H S G++ + T+ LL EH F+ +A+
Sbjct: 53 GFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHIALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEEYISNAAHMLSRLEELQDVLAKKELPQDLEGARNMIDEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSG-SG----------NADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKAC---GEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSS------------------DSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDH-----VTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRN---SVSMPGMVTHIKAPEQQVKN----------ILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTS-TGSSIQHTQELLKEHEEFQITAK 1190 HSP 2 Score: 115.931 bits (289), Expect = 1.295e-24 Identity = 160/764 (20.94%), Postives = 327/764 (42.80%), Query Frame = 0
Query: 330 TASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKL-LYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVL-------DWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGE---CN--------AEDIYSVAHELESHISSFAQRVEERRKLLELAIAFY---SHTEELISWLSELKQELQSEEVS-ESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTR----KMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHI----AHIQSATYEVAQRGQEL-LKILENSGLTL--MSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWI-KSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEE 1055
++ ++T + + ++ ++ ++ V Q+QL +WQ +K +L Q QL +F+QD +++ DWI +H FL + +G+S ++ LQ H F + N ++N +++L A +L ++ + + A +L+ +DF +++R +L +SV FH +V + W + Q + L + +S V K+ Q L T + I+ GE + R S + K ++ +H+ V+ + + +E + +KIKL L LR+F+ D ++ D L F +++ I Q+ + + T + + + LL E+ +G C+ +D+ HE + + A E+ RK LE + + ++++ W+ + L + ++ SL+ AE+L EQF + + T + + + +L+ +N M++ V S +L + ++ ++ N K +C L E+ +Y+ + D ET+ +S+H+ A +++ T +A+R ++ LK L + +++ M + +V+ +L + +RE + + R+ +L+QC Q FE A Q + WI + E L+ S +Q +++ KEHE+FQ ++T + Q A+ + H + +K+ V R++ R E+
Sbjct: 520 SSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHT----HVKELWTWLEEL--------------------QKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTP----HNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAE--QRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAA---EQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQ---ANHYDMDM-IRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQ-------EYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACT--LARRNADVFLKYLHRNSVSMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEK 1237 HSP 3 Score: 92.8189 bits (229), Expect = 1.747e-17 Identity = 89/441 (20.18%), Postives = 180/441 (40.82%), Query Frame = 0
Query: 319 SYDSGYSGRD---------STASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTK----------WSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAV---IHNILDHHRTLETK----WHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
++D + G+D ++ + C+ D+ + + +L+ +H QQ+L + + L+QC QLR + + +++ WI + L+ + S QE+++LQ +H F + ++ A ++++NHY +R A ++ +W+ +++R ++ SV F++ ++Q + W G D S ++ + I +H E ++ T + L K+ R+S P +H+ A + NIL+ E + W +K +L Q +F+ KQ L+W+ ++GE +L + G S+Q + K HE F+ + T + L+ A + G +A +I ++ F+ R+E+ R LE A+
Sbjct: 842 TFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRV-QDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGE-SMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPM---ISKHLEQKEAFLKACTLARRNADVFL-------------------KYLHRNSVSMPGM----------VTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGI 1248
BLAST of triple functional domain partial vs. SwissProt
Match: gi|123917562|sp|Q1LUA6.1|TRIO_DANRE (RecName: Full=Triple functional domain protein) HSP 1 Score: 1001.89 bits (2589), Expect = 0.000e+0 Identity = 522/1190 (43.87%), Postives = 760/1190 (63.87%), Query Frame = 0
Query: 85 LRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTA------SNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-ELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL---QNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKI---LENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRS-----TGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
++A++V+ +L+E++A LSGGRD+RGG ++TF + +R R ED + L+ YL G+P EDV GF VI+DMRGS W ++KP+LK+LQE F IH IIKP+NFWQKQRT+ GS K++FET +SL+ L KV+DPS LT D +G+L+Y+H WIE+R A EDF + ++ RL+ M E + D D++GAR+ E H +K++V+K PVE +DN GQ LLQR + E Y+ R N D Q +P I +L+ +HS +Q L Q W I+K +LDQC QLRLFEQD EKMFDWI H++ FL Y IG + + +LQ HNHF M NV+ NINRI+S +L+E+ HYASQ ++ ++ +L++ WK FA LDER+ +L +S FHQK QY+ +V W + C + +++ LE +IH HQ LYE + +Y+EV K LL +L + SA + +Y++ +HVL +IH +L H R LE W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK HE FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY AH+LE I F +RVE+R+ LL++++AF++H +EL +WL EL++EL + +ES+E + LI++F Q+ +TL + I EG+ L+++L+ I+ + + S+N ++S L +L + ++ E++ RK++L+L QLR+FERDA+++ S + W E+L N D+ E +L++ ++ + + + V +GQELL+ ++ SG+ L+ D + +RVQ LLE++ E++ +L+ Q R LEQC+QL + + QV+ WI++ E ML AG LQ+AEQ+++EHEQFQ AIEKTH SA+ V+Q+AEALLQ NH++ +++ A+ V + WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EY+R++DW G + SDH V +I+KH EQKEAFLKACTLARR A+ FLKY +R+ G + K PE +VK IL LL +ENRVL +WT +K+RL+Q QQ+ +FERSAKQ+L WI + G+ YLS+H S G++ T+ LL EH F +A+
Sbjct: 1 MKAMEVLPILKEKVAFLSGGRDKRGGPVLTFPSRTNHDRIRHEDLRRLIAYLAGIPSEDVCKHGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHIALIIKPDNFWQKQRTNFGSSKFEFETTMVSLEGLSKVVDPSQLTADFEGSLDYNHEEWIEIRVAFEDFTGNARHLLARLEEMHETVTRKDLPQDLDGARRMIEEHAALKKRVIKAPVEEVDNEGQRLLQRIQSSE-------SYANRTVPVPPGQREGQGQPNADTQGLVPRITALLEKLHSTRQNLHQSWHIRKLQLDQCFQLRLFEQDAEKMFDWIMHNKGLFLAGYTEIGNNHPHAMELQTQHNHFAMNCMNVYVNINRIMSVGNRLLEAGHYASQQIKQISGQLEQEWKAFAAALDERSALLEMSATFHQKCDQYMSNVDSWCKAC---GEVDLPSELQELEDAIHHHQGLYEHITAAYSEVSQDGKALLDKLQRPLTPGSA------------------DSLTASANYTKAVNHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQFTVNVIKEGEDLIQQLRDLAISSNKTPHNSSINHIESVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLDSWNEELSQQMNEFDTEDLTLAEQRLQHHADKALTMNNLAFHVIHQGQELLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDVAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEALLQANHYDMDMIRDCAENVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNCESDH-------VTPMISKHLEQKEAFLKACTLARRNADVFLKYMHRN---SVSMPGMLSHVKAPEQQVKN----------ILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTS-TGSSIHHTQELLKEHEDFHITAK 1141 HSP 2 Score: 106.686 bits (265), Expect = 9.398e-22 Identity = 154/762 (20.21%), Postives = 326/762 (42.78%), Query Frame = 0
Query: 330 TASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKL-LYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRK-----NVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGE---CN--------AEDIYSVAHELESHISSFAQRVEERRKLLELAIAFY---SHTEELISWLSELKQELQSEEVS-ESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTR----KMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHI----AHIQSATYEVAQRGQEL-LKILENSGLTL--MSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWI-KSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEE 1055
+A ++T + + ++ ++ ++ V Q+QL +WQ +K +L Q QL +F+QD +++ DWI +H FL + +G+S ++ LQ H F + N ++N +++L A +L ++ + + A +L+ +DF +++R +L +SV FH +V + W + Q + L + +S V K+ Q L T + I+ GE + R + K ++ +H+ +V+ + + +E + +KIKL L LR+F+ D ++ L + E ++ + + Q+ + + T + + ++LL E+ +G C+ +D+ HE + + A E+ R+ LE + + ++++ W+ + L + ++ SL+ AE+L EQF + + T + + + LL+ +N M++ + N V S +L + ++ ++ N K +C L E+ +Y+ + D E++ +S+H+ A +++ T +A+R ++ LK + + +++ M + +V+ +L + +RE + + R+ +L+QC Q FE A Q + WI + E L+ S + +++ KEHE F ++T + Q A+ H + +K+ V R++ R ++
Sbjct: 471 SADSLTASANYTKAVNHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHT----HVKELWTWLEEL--------------------QKELLDDVYAESVEAVQDLIKRFGQQQQTTLQFTVNVIKEGEDLIQQLRDLAISSNKTP----HNSSINHIESVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLDSWNEELSQQMNEFDTEDLTLAEQRLQHHADKALTMNNLAFHVIHQGQELLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDVAA---EQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEALLQ---ANHYDMDMIRDCAEN-VASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQ-------EYKREEDWCGGADKLGPNCESDHVTPMISKHLEQKEAFLKACT--LARRNADVFLKYMHRNSVSMPGMLSHVKAPEQQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFHITAKQTKERVKLLIQLADGFCDKGHSHAAEIKKWVTAVDKRYRDFSLRMDK 1188 HSP 3 Score: 87.4261 bits (215), Expect = 6.262e-16 Identity = 112/546 (20.51%), Postives = 218/546 (39.93%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNH--NDF-ADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTK----------WSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAV---IHNILDHHRTLETK----WHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAE 764
+EL L F + +++ LD E+L+ N+F +D+ A + + H + + L I GQ LLQ + + ++ + C+ D+ + + +L+ +H QQ+L + + L+QC QLR + + +++ WI + L+ + S QE+++LQ +H F + ++ A L+++NHY +R A + +W+ +++R ++ SV F++ ++Q + W G D + ++ + I +H E ++ T + L K+ R+S P SHV A + NIL+ E + W +K +L Q +F+ KQ L+W+ + GE +L + G S+ + K HE F + T + L+ A G +A +I ++ F+ R+++ R LE A+ S + ++ ++LQ + + S G+E
Sbjct: 733 LELFLQLRIFERDAIDIISDLDSWNEELSQQMNEFDTEDLTLAEQRLQHHADKALTMNNLAFHVIHQ-GQELLQYVNEVQ-------------ASGVELLCDRDVDMATRV-QDLLEFLHEKQQELDVAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGE-SMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEALLQANHYDMDMIRDCAENVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNCESDHVTPM---ISKHLEQKEAFLKACTLARRNADVFL-------------------KYMHRNSVSMPGM----------LSHVKAPEQQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFHITAKQTKERVKLLIQLADGFCDKGHSHAAEIKKWVTAVDKRYRDFSLRMDKYRTSLEKALGISSDS-------NKASKDLQLDIIPASAPGSE 1223
BLAST of triple functional domain partial vs. SwissProt
Match: gi|160011671|sp|A2CG49.1|KALRN_MOUSE (RecName: Full=Kalirin; AltName: Full=Protein Duo; AltName: Full=Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain) HSP 1 Score: 993.03 bits (2566), Expect = 0.000e+0 Identity = 535/1207 (44.32%), Postives = 778/1207 (64.46%), Query Frame = 0
Query: 76 IGGHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-----ELDASGSMNSV---------QSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL--QNGDL-PRDVKETESQLRNLSEHIAHIQSATYEVAQRGQEL---LKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG-----RSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASF-KGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
+ G +R D L+A DV+ +L+E++A +SGGRD+RGG I+TF A +R R ED + L+ YL VP EDV GF VIIDMRGS W +KP+LK LQE F +IH IIKP+NFWQKQ+T+ GS K+ FET+ +S++ L K++DPS LT++ DG+L+Y+H WIELR +LE+F + ++ RL+ ++E L +F DV G+R+ + H ++K+KVLK PVE +D GQ LLQ + G+SGR+ + D Q+ +P I +LD +HS +Q L Q+W ++K KLDQC QLRLFEQD EKMFDWI H++ FL ++ IG S+Q + LQ HNHF M S N + NINRI+S A++L E+ HYASQ ++ ++ +LD+ WK FA LDER+T+L +S +FHQKA+Q++ V W C M S +++ LE +IH HQSLYE + Q+YTEV K LL ++Q + N E + +YS+ VL V+H +L H R LE+ W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK H+ FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY A LE I F +RVE+R+ LL+++++F++HT+EL +W+ +L++E+ + ++S++ + LI+QF Q+ +TLDA + I EG+ L+++L+S ++ D++ S N +S L +L + ++ E+++ RK++LD+ QLR+FE+ +E++++ + W E L Q D D+ E +L+ +E + + T+EV Q+GQ+L + ++ SG+ L+ + + ++VQ LLE++ E++ +LE Q +LEQC+QL + + QV+ WI++ E ML A L +AEQ+++EHEQFQ AIEKTH SA+ V+Q+AEALLQ H++ ++E A+ V WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EYRRD+DW G DH V L++KH EQKEAFLKACTLARR AE FLKY +R+ ++ S AS +GPE +VK IL LL +ENRVL +WT KK+RL+Q QQ+ +FERSAKQ+L WI+E G+ YLS+H S T EET+ LL E+ F+ A+
Sbjct: 3 LSGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSD-------GFSGRNCIPGSA----DFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAW---CKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALL----DVLQRPLSPGNSE--------------SLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDH-------VIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRN----NVSMPSVASHTRGPEQQVKA----------ILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTG-ETTEETQELLKEYGEFRVPAK 1155
BLAST of triple functional domain partial vs. SwissProt
Match: gi|160380715|sp|P97924.3|KALRN_RAT (RecName: Full=Kalirin; AltName: Full=Huntingtin-associated protein-interacting protein; AltName: Full=PAM COOH-terminal interactor protein 10; Short=P-CIP10; AltName: Full=Protein Duo; AltName: Full=Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain) HSP 1 Score: 989.564 bits (2557), Expect = 0.000e+0 Identity = 534/1207 (44.24%), Postives = 777/1207 (64.37%), Query Frame = 0
Query: 76 IGGHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-----ELDASGSMNSV---------QSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL--QNGDL-PRDVKETESQLRNLSEHIAHIQSATYEVAQRGQEL---LKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG-----RSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASF-KGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
+ G +R D L+A DV+ +L+E++A +SGGRD+RGG I+TF A +R R ED + L+ L VP EDV GF VIIDMRGS W +KP+LK LQE F +IH IIKP+NFWQKQ+T+ GS K+ FET+ +S++ L K++DPS LT++ DG+L+Y+H WIELR +LE+F + ++ RL+ ++E L +F DV G+R+ + H ++K+KVLK PVE +D GQ LLQ + G+SGR+ + D Q+ +P I +LD +HS +Q L Q+W ++K KLDQC QLRLFEQD EKMFDWI H++ FL ++ IG S+Q + LQ HNHF M S N + NINRI+S A++L E+ HYASQ ++ ++ +LD+ WK FA LDER+T+L +S +FHQKA+Q++ V W C M S +++ LE +IH HQSLYE + Q+YTEV K LL ++Q + N E + +YS+ VL V+H +L H R LE+ W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK H+ FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY A LE I F +RVE+R+ LL+++++F++HT+EL +W+ +L++E+ + ++S++ + LI+QF Q+ +TLDA + I EG+ L+++L+S ++ D++ S N +S L +L + ++ E+++ RK++LD+ QLR+FE+ +E++++ + W E L Q D D+ E +L+ +E + + T+EV Q+GQ+L + ++ SG+ L+ + + ++VQ LLE++ E++ +LE Q +LEQC+QL + + QV+ WI++ E ML A L +AEQ+++EHEQFQ AIEKTH SA+ V+Q+AEALLQ H++ ++E A+ V WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EYRRD+DW G DH V L++KH EQKEAFLKACTLARR AE FLKY +R+ ++ S AS +GPE +VK IL LL +ENRVL +WT KK+RL+Q QQ+ +FERSAKQ+L WI+E G+ YLS+H S T EET+ LL E+ F+ A+
Sbjct: 3 LSGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARTNHDRIRQEDLRKLVTNLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSD-------GFSGRNCIPGSA----DFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAW---CKMCSEGGLPSEMQDLELAIHHHQSLYEQVTQAYTEVSQDGKALL----DVLQRPLSPGNSE--------------SLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAVSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDVDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIEKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEMDH-------VIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRN----NVSMPSVASHTRGPEQQVKA----------ILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEYYLSTHTSTG-ETTEETQELLKEYGEFRVPAK 1155
BLAST of triple functional domain partial vs. SwissProt
Match: gi|160380714|sp|O60229.2|KALRN_HUMAN (RecName: Full=Kalirin; AltName: Full=Huntingtin-associated protein-interacting protein; AltName: Full=Protein Duo; AltName: Full=Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain) HSP 1 Score: 983.786 bits (2542), Expect = 0.000e+0 Identity = 534/1214 (43.99%), Postives = 776/1214 (63.92%), Query Frame = 0
Query: 78 GHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITM-----ELDASGSMNSV---------QSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQL--QNGDL-PRDVKETESQLRNLSEHIAHIQSATYEVAQRGQEL---LKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIE---------KTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLG-----RSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASF-KGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G +R D L+A DV+ +L+E++A +SGGRD+RGG I+TF A +R R ED + L+ YL VP EDV GF VIIDMRGS W +KP+LK LQE F +IH IIKP+NFWQKQ+T+ GS K+ FET+ +S++ L K++DPS LT++ DG+L+Y+H WIELR +LE+F + ++ RL+ ++E L +F DV G+R+ + H ++K+KVLK PVE +D GQ LLQ + G+SGR+ + D Q+ +P I +LD +HS +Q L Q+W ++K KLDQC QLRLFEQD EKMFDWI H++ FL ++ IG S+Q + LQ HNHF M S N + NINRI+S A++L E+ HYASQ ++ ++ +LD+ WK FA LDER+T+L +S +FHQKA+Q++ V W C M S +++ LE +IH HQ+LYE + Q+YTEV K LL ++Q + N E + +YS+ VL V+H +L H R LE+ W +K++LHQRL L +FQ+DV+QVLDW++NHGE FL K+ G+G+SL +ARA QK H+ FE V QNTYTNA+KLL AA +LAQTGEC+ E+IY A LE I F +RVE+R+ LL+++++F++HT+EL +W+ +L++E+ + ++S++ + LI+QF Q+ +TLDA + I EG+ L+++L+S ++ D++ S N +S L +L + ++ E+++ RK++LD+ QLR+FE+ +E++++ + W E L Q D D+ E +L+ +E + + T+EV Q+GQ+L + ++ SG+ L+ + + ++VQ LLE++ E++ +LE Q +LEQC+QL + + QV+ WI++ E ML A L +AEQ+++EHEQFQ AIE KTH SA+ V+Q+AE LLQ H++ ++E A+ V WQ L+ + E+R KLV AS+ FYKT+EQVCSVL+SLE+EYRRD+DW G DH V LI+KH EQKEAFLKACTLARR AE FLKY +R+ ++ S AS +GPE +VK IL LL +ENRVL +WT KK+RL+Q QQ+ +FERSAKQ+L WI+E G+ YLS+H S T EET+ LL E+ F+ A+
Sbjct: 23 GSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRCSD-------GFSGRNCIPGSA----DFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQYALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAW---CKMCSEGGLPSEMQDLELAIHHHQTLYEQVTQAYTEVSQDGKALL----DVLQRPLSPGNSE--------------SLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEMLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPSEARDSAVSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYITEVQASGIELICEKDIDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGGRDKLGPAAEIDH-------VIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRN----NVSMPSVASHTRGPEQQVKA----------ILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGEFYLSTHTSTG-ETTEETQELLKEYGEFRVPAK 1182
BLAST of triple functional domain partial vs. SwissProt
Match: gi|82184254|sp|Q6GNM9.1|SES1B_XENLA (RecName: Full=SEC14 domain and spectrin repeat-containing protein 1-B) HSP 1 Score: 181.03 bits (458), Expect = 3.467e-46 Identity = 172/697 (24.68%), Postives = 301/697 (43.19%), Query Frame = 0
Query: 85 LRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYK----FETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPV---ENIDNVGQLLL-----QRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQ--IKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNN---VFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSY-TEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGA 763
+ A ++ +L+++LA LSGG+DRR G I+T ++ ++ L YLL +P E + GF VI+D R S W VK ++ +LQ ++ V ++KP+ FW K+ T K K FE +S + L + I+P+ LT++ G+L YDH W+ R E F +ST ++D L V+ NG+ K ++ E LP E + G LL +RF+ +GG S+ S M D + P +M++LD++ + ++ + K+++LD+ +Q ++ W+ + L IG S + S+ LQ H + V+ +N+ + AA L + ++ + RL A L+ R +L +++ FH +Q SQ D M A T ++I Q L E +S T + +K LD + +S KD + + H+ V+ ++ + E +++K+ Q + L +ED Q +DWL + L+ ++ +G Q+ + + H F V Q+TY +LL A L Q+ C + +L F EER + LE+A+AF+S+ E+++ +L + + E + +E
Sbjct: 1 MDATVILPILKKKLAFLSGGKDRRSGLILTIPLCS--DQTSMDELSVTLDYLLSIPSEKCKARGFTVIVDGRKSQWNVVKTVVLMLQNVVPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSGNKLTRYIEPNQLTEEFGGSLTYDHMDWLNKRLVFEKFTKESTSLLDELAVIN------------NGSDKGSQNEKERSVDFNYLPSVDPETVLQTGHELLSELQQRRFNGSDGGVSW-----------SPM---DDELLAQPQVMKLLDSLREQYTRYQEVCRQRSKRTQLDE------IQQKVMQVVSWLEGPGSEQLRTQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELNQQI--AALLNAGDEEDLVELKTLQQRLSDVCYRQASQLEFRQNLLQIALEFHSVSQDL-------SQQLDGLLGMLCADVAPTDGATIQQTLKLLEEKLKSVDTGLQGLREKGQGLLDQITNQASWAYG-----------------KDVSTENKDNVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQAVDWLNELLDALLKTHIRLGDDSQETKILLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPKLNRVWKQFTITSEERVQRLEMALAFHSNAEKILQECPDLGETVMDFEQFDEVEAV 637
BLAST of triple functional domain partial vs. SwissProt
Match: gi|81873131|sp|Q80UK0.1|SESD1_MOUSE (RecName: Full=SEC14 domain and spectrin repeat-containing protein 1; AltName: Full=Huntingtin-interacting protein-like protein) HSP 1 Score: 179.489 bits (454), Expect = 1.785e-45 Identity = 170/691 (24.60%), Postives = 287/691 (41.53%), Query Frame = 0
Query: 85 LRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYK----FETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPV---ENIDNVGQLLL-----QRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYAS-QHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEG 762
+ A ++ +L+++LA LSGG+DRR G I+T E+ ++ L YLL +P E + GF VI+D R S W VK ++ +LQ ++ V ++KP+ FW K+ T K K FE +S + L + I+P LT+D G+L YDH W+ R E F +ST ++D L ++ NG+ K E E + LP E + G LL +RF+ +GG S+ S M D + P +M++LD++ + ++ + + + QL +Q ++ +W+ + L IG S + S+ LQ H + F+ + A L+ + ++ + +L A L+ R +L ++ FH AQ SQ D M +SI Q L E +S V + G+ + + KD + E H+ V+ ++ + E +++K+ Q + L +ED Q ++WL + L+ ++ +G Q+ + + H F V Q+TY +LL A L Q+ C + L F EER LE+AIAF+S+ E+++ E + + EE E +E
Sbjct: 1 MEASVILPILKKKLAFLSGGKDRRSGLILTIPLC--LEQTSMDELSVTLDYLLSIPSEKCKARGFTVIVDGRKSQWNVVKTVVLMLQNVVPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQLTEDFGGSLTYDHMDWLNKRLVFEKFTKESTSLLDELALIN------------NGSDKGNEQEKERSVDLNFLPSVDPETVLQTGHELLSELQQRRFNGSDGGVSW-----------SPMD---DELLAQPQVMKLLDSLREQYTRYQEVCRQRSKR----TQLEEIQQKVMQVVNWLEGPGSEQLRAQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELNQQIAALLNAGDEEDLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDL-------SQQLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVD----------------VGLQGLREKGQGLLDQISNQASWAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDASQAVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPRLNRVWKQFTVASEERVHRLEMAIAFHSNAEKILQDCPEEPEAMNDEEQFEEIEA 636
BLAST of triple functional domain partial vs. SwissProt
Match: gi|82184515|sp|Q6GPX2.1|SES1A_XENLA (RecName: Full=SEC14 domain and spectrin repeat-containing protein 1-A) HSP 1 Score: 178.718 bits (452), Expect = 2.917e-45 Identity = 169/690 (24.49%), Postives = 292/690 (42.32%), Query Frame = 0
Query: 90 VMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYK----FETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPV---ENIDNVGQLLL-----QRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLW--QIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYAS-QHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSY-TEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGA 763
++ +L+++LA LSGG+DRR G I+T ++ ++ L YLL +P E + GF VI+D R S W VK ++ +LQ ++ V ++KP+ FW K+ T K K FE +S + L + I+PS LT+ G+L YDH W+ R E F +ST ++D L V+ NG+ K ++ E LP E + G LL +RF+ +GG S+ S M D + P +M++LD++ + ++ + K+++LD+ +Q ++ W+ + L IG SF+ S+ Q H + F+ + A L+ + ++ + RL A L+ R +L +++ FH AQ SQ D M + S+I Q L E +S T + K LD + ++ KD + + H+ V+ ++ + E +++K+ Q + L +ED Q +DWL + L+ ++ +G Q+ + + H F V Q+TY +LL A L Q+ C + +L F EER LE+A+AF+S+ E+++ +L + + E + +E
Sbjct: 6 ILPILKKKLAFLSGGKDRRSGLILTIPLCS--DQTSMDELSVTLDYLLSIPSEKCKARGFTVIVDGRKSQWNVVKTVVLMLQNVVPAEVSLVCVVKPDEFWDKKVTHFCFWKEKDRLGFEVILVSGNKLTRYIEPSQLTEQFGGSLTYDHMDWLNKRLVFEKFTKESTSLLDELAVIN------------NGSDKGSQDEKERSVDFNYLPSVDPETVLQTGHELLSELQQRRFNGSDGGVSW-----------SPMD---DELLAQPQVMKLLDSLREQYTRYQEVCRQRSKRTQLDE------IQQKVMQVVGWLEGPGSEQLRTQWGIGDSFRASQAFQQKHEEIESQHSEWFAVYVELNQQIAALLNAGDEEDLVELKTLQQRLSDVCYRQASQLEFRQNLLQIALEFHSVAQDL-------SQQLDGLLGMLCVDVAPSDGSAIQQTLKLLEEKLKSVDTGLQGLRDKGQGLLDQITNQATWAYG-----------------KDVSTENKDNVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQAVDWLNELLDALLKTHIRLGDDSQETKILLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPKLNRVWKQFTITSEERVHRLEMALAFHSNAEKILQECPDLGEAVMEFEQFDEVEAV 637
BLAST of triple functional domain partial vs. nr
Match: gi|1325254111|ref|XP_023330642.1| (triple functional domain protein-like isoform X4 [Eurytemora affinis]) HSP 1 Score: 1706.42 bits (4418), Expect = 0.000e+0 Identity = 842/1246 (67.58%), Postives = 989/1246 (79.37%), Query Frame = 0
Query: 23 HPHGYAGAVPKHG---GSSHARSAQGVPQASGGHHGPLTRAPAPSATDPVAQIRRPIGGHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRG-STWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSS-AIQNGEHKKHES-----RHSGVPPPKK-DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRD-VKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
H HG A P HG G SH G HGPL P + + G R D LR +DVM + E LA+L+GGRDRRGG ++T PRRERARPED+K LL YLLGVPC+DVR++GF V+IDMRG S+WTTVKPILKVLQ+ ++ IH+ IIKP+ FWQKQRT+LGSQKYKFET+ I L+SL K +DPS LT DL+G L+YDHT WIE+RC LEDF+WQS+E++DR++ MREDLNHNDFADDVNGA+K+ ++HNE K+K+ K+PVENID +GQ LLQR STCEGGNSYDSGY GRDSTASNMT NPD+Q +IP IMQ+LD VHSGQQQLLQLW++K++KLD C QLR+FEQDCEKMFDWI +R FL NYV IGR++Q++K +Q++HN FT SNNV+ NI+RIL+ A KLIE HYA+ HVR VA+RLD WK FAG LDERTTVL LSVLFHQKA+ YV++V+ WS GC++ ++DI+ LE+SIH HQ+LYE MCQ+YTEVHSTSKKLLYQLDHLVQ S+ +Q KKH S R PPPK D DY EGA HVL+VIH IL HHR LE KWH KKIKLHQRLALRLFQEDVKQVLDWL+ HGEVFLRKNVGIGR+LQK+R YQKSHETFEGVVQNTYTNAEKLLAAA ELAQTGECNAEDIYSVA ELE+HI+SFA RVE RR +L+ A+ FYSH EEL SWL+ELK EL SEEV+E+ E AERL++QFS QR+STLDA STI EG++LL EL GI E+D++GS+ SVQSTL RL +R+ELGE+W TRK+RLDLC QLRLFERDALELS+Q+E+W E+L G LPR+ V++ E R L + I+HIQSATY V QRGQ+LL++ E+SG+ LM+DSQYNG++RVQVLLEY+QER +D+EEL + RR+KLEQC+ L+ ++ DA+QV+RWI++ E ML A FSIPG L DAE +KKEHEQFQ AIEKTH+SAVHVRQRAE LLQ NHF+P VKEIAD VT RWQ LVTRAEERHKLVTASLNFYKTAEQVCSVLDSLE+EY+R+DDWL R T HD+ L+AQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYF E ++GPE KVK ILE+LLTQENRVLEYWT KKKRL+QNQQ+ LFERSA+QSLAWIKEEGDIYLS+H ++VG KEETE LL EHNSFK AR
Sbjct: 29 HQHGPGVASPPHGAIGGQSH------------GLHGPLAHRGGPGGGKEEGKFL--LSGARRVDGLRCLDVMKEMEEGLALLTGGRDRRGGPVLTLPQNPRRERARPEDYKKLLDYLLGVPCDDVREMGFTVVIDMRGNSSWTTVKPILKVLQDNYSTSIHTALIIKPDTFWQKQRTNLGSQKYKFETHLIGLESLHKQVDPSQLTVDLEGTLSYDHTIWIEMRCGLEDFVWQSSELLDRMEDMREDLNHNDFADDVNGAKKSMDLHNESKKKIHKVPVENIDGMGQRLLQRLSTCEGGNSYDSGYCGRDSTASNMTLNPDLQVAIPQIMQLLDQVHSGQQQLLQLWKVKRNKLDHCLQLRMFEQDCEKMFDWIFSNRDVFLGNYVEIGRNYQDAKTIQEEHNQFTAASNNVYVNISRILTAAGKLIEGGHYAAVHVRDVASRLDSTWKGFAGGLDERTTVLALSVLFHQKAETYVENVSGWSGGCEV---QGVSSDITVLETSIHHHQALYEAMCQAYTEVHSTSKKLLYQLDHLVQVSAQTVQESPQKKHSSGCPQKRKEPRPPPKSTDPAADYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYSHVEELGSWLAELKGELASEEVAETAENAERLLDQFSTQRESTLDALQSTIQEGESLLRELGEQGIDQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGD---HDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFNHPNHPE--YRGPELKVKN----------ILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTH-TQVGKNKEETEVLLQEHNSFKEKAR 1241 HSP 2 Score: 97.0561 bits (240), Expect = 2.675e-16 Identity = 89/436 (20.41%), Postives = 185/436 (42.43%), Query Frame = 0
Query: 304 GQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTKWSQGCD--MTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
GQ LLQ F + DS Y+G + +L+ + + +L +++ KL+QC L ++ D ++ WI + ++ SI S +++ L+ +H F + S+ + A L++ NH+ V+ +A + W++ +ER ++ S+ F++ A+Q +DS+ + + D + S D +TL + I++HQ E ++ T T++ L ++ ++Q H H +Y V ++ +L + W KK +L Q LF+ +Q L W++ G+++L + +G++ ++ + H +F+ + T + LL A L + G +A +I E++ +F+ R+++ R+ LE ++
Sbjct: 903 GQDLLQLFESSGINLMADSQYNGETR-----------------VQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASF-SIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKY------SNRSLQYFNHPNHP---------------EYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGL 1299 HSP 3 Score: 95.9005 bits (237), Expect = 6.270e-16 Identity = 160/724 (22.10%), Postives = 295/724 (40.75%), Query Frame = 0
Query: 339 DMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEK---LL----AAAAELAQTGECNAEDIYSVAHE-LESHISSFAQRVEERRKLLELAIA---FYSHTEELISWLSELKQELQ-SEEVSESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDS---QYNG-QSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKS-AEGMLAAGFSIPGCLQDAEQIKKEHEQFQ------------------TAIEKTHASAVHVRQRAEAL 1024
D Q ++ ++ + + +QL W KK KL Q LRLF++D +++ DW+ H FL V IGR+ Q+S+ Q H F N ++N ++L+ A +L ++ ++ + VA L+ + FA ++ R +L +VLF+ +V+ + W ++ +++ T E++ + Q T+ ST LD L S IQ GE E G+ D D + + V + + + L W +K++L L LRLF+ D ++ + GE + RA ++ + + TY+ ++ LL ++ L + N E V E L+ + RR LE ++ + S +++ W+ + L S + SL AE L EQF + T + +TLL+ + ++ A N Q+ + R + + N K +C L ER+ + + W + +GD R + ++ + A +++ T +A+R E N L + +Y G + +V+ +LE + +E + E + ++ +L+Q Q FE A Q + WIK + L+ + ++ E + +EH F+ T +EK HA A +++Q E +
Sbjct: 586 DYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYS----HVEELGSWL--AELKGELASEEVAETAENA-------ERLLDQFSTQREST-------LDAL---QSTIQEGESLLRELGEQGI-----DQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREY----KREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACT--LARRTAETFLKYSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEV 1275 HSP 4 Score: 65.4698 bits (158), Expect = 9.244e-7 Identity = 40/165 (24.24%), Postives = 79/165 (47.88%), Query Frame = 0
Query: 324 YSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDER----TTVLGLSV 484
YS R N +P+ + + +L+ + + + ++L+ W KK +LDQ Q LFE+ + WI +L + +G++ +E++ L +HN F + + +L A L+E H + +++ +D+ +K+F+ +D+ LGLSV
Sbjct: 1137 YSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGLSV 1301
BLAST of triple functional domain partial vs. nr
Match: gi|1325254094|ref|XP_023330411.1| (triple functional domain protein-like isoform X2 [Eurytemora affinis]) HSP 1 Score: 1701.8 bits (4406), Expect = 0.000e+0 Identity = 830/1206 (68.82%), Postives = 980/1206 (81.26%), Query Frame = 0
Query: 55 GPLTRAPAPSATDPVAQIRRPIGGHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRG-STWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSS-AIQNGEHKKHESRHSGVPPPKK-DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRD-VKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G L+R+ P + + + G R D LR +DVM + E LA+L+GGRDRRGG ++T PRRERARPED+K LL YLLGVPC+DVR++GF V+IDMRG S+WTTVKPILKVLQ+ ++ IH+ IIKP+ FWQKQRT+LGSQKYKFET+ I L+SL K +DPS LT DL+G L+YDHT WIE+RC LEDF+WQS+E++DR++ MREDLNHNDFADDVNGA+K+ ++HNE K+K+ K+PVENID +GQ LLQR STCEGGNSYDSGY GRDSTASNMT NPD+Q +IP IMQ+LD VHSGQQQLLQLW++K++KLD C QLR+FEQDCEKMFDWI +R FL NYV IGR++Q++K +Q++HN FT SNNV+ NI+RIL+ A KLIE HYA+ HVR VA+RLD WK FAG LDERTTVL LSVLFHQKA+ YV++V+ WS GC++ ++DI+ LE+SIH HQ+LYE MCQ+YTEVHSTSKKLLYQLDHLVQ S+ +Q KKHE R PPPK D DY EGA HVL+VIH IL HHR LE KWH KKIKLHQRLALRLFQEDVKQVLDWL+ HGEVFLRKNVGIGR+LQK+R YQKSHETFEGVVQNTYTNAEKLLAAA ELAQTGECNAEDIYSVA ELE+HI+SFA RVE RR +L+ A+ FYSH EEL SWL+ELK EL SEEV+E+ E AERL++QFS QR+STLDA STI EG++LL EL GI E+D++GS+ SVQSTL RL +R+ELGE+W TRK+RLDLC QLRLFERDALELS+Q+E+W E+L G LPR+ V++ E R L + I+HIQSATY V QRGQ+LL++ E+SG+ LM+DSQYNG++RVQVLLEY+QER +D+EEL + RR+KLEQC+ L+ ++ DA+QV+RWI++ E ML A FSIPG L DAE +KKEHEQFQ AIEKTH+SAVHVRQRAE LLQ NHF+P VKEIAD VT RWQ LVTRAEERHKLVTASLNFYKTAEQVCSVLDSLE+EY+R+DDWL R T HD+ L+AQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYF E ++GPE KVK ILE+LLTQENRVLEYWT KKKRL+QNQQ+ LFERSA+QSLAWIKEEGDIYLS+H ++VG KEETE LL EHNSFK AR
Sbjct: 80 GNLSRSSPP---EDLEYLNNNPDGARRVDGLRCLDVMKEMEEGLALLTGGRDRRGGPVLTLPQNPRRERARPEDYKKLLDYLLGVPCDDVREMGFTVVIDMRGNSSWTTVKPILKVLQDNYSTSIHTALIIKPDTFWQKQRTNLGSQKYKFETHLIGLESLHKQVDPSQLTVDLEGTLSYDHTIWIEMRCGLEDFVWQSSELLDRMEDMREDLNHNDFADDVNGAKKSMDLHNESKKKIHKVPVENIDGMGQRLLQRLSTCEGGNSYDSGYCGRDSTASNMTLNPDLQVAIPQIMQLLDQVHSGQQQLLQLWKVKRNKLDHCLQLRMFEQDCEKMFDWIFSNRDVFLGNYVEIGRNYQDAKTIQEEHNQFTAASNNVYVNISRILTAAGKLIEGGHYAAVHVRDVASRLDSTWKGFAGGLDERTTVLALSVLFHQKAETYVENVSGWSGGCEV---QGVSSDITVLETSIHHHQALYEAMCQAYTEVHSTSKKLLYQLDHLVQVSAQTVQESPQKKHEPR----PPPKSTDPAADYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYSHVEELGSWLAELKGELASEEVAETAENAERLLDQFSTQRESTLDALQSTIQEGESLLRELGEQGIDQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGD---HDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFNHPNHPE--YRGPELKVKN----------ILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTH-TQVGKNKEETEVLLQEHNSFKEKAR 1259 HSP 2 Score: 97.4413 bits (241), Expect = 2.169e-16 Identity = 89/436 (20.41%), Postives = 185/436 (42.43%), Query Frame = 0
Query: 304 GQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTKWSQGCD--MTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
GQ LLQ F + DS Y+G + +L+ + + +L +++ KL+QC L ++ D ++ WI + ++ SI S +++ L+ +H F + S+ + A L++ NH+ V+ +A + W++ +ER ++ S+ F++ A+Q +DS+ + + D + S D +TL + I++HQ E ++ T T++ L ++ ++Q H H +Y V ++ +L + W KK +L Q LF+ +Q L W++ G+++L + +G++ ++ + H +F+ + T + LL A L + G +A +I E++ +F+ R+++ R+ LE ++
Sbjct: 921 GQDLLQLFESSGINLMADSQYNGETR-----------------VQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASF-SIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKY------SNRSLQYFNHPNHP---------------EYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGL 1317 HSP 3 Score: 95.5153 bits (236), Expect = 6.714e-16 Identity = 160/724 (22.10%), Postives = 295/724 (40.75%), Query Frame = 0
Query: 339 DMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEK---LL----AAAAELAQTGECNAEDIYSVAHE-LESHISSFAQRVEERRKLLELAIA---FYSHTEELISWLSELKQELQ-SEEVSESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDS---QYNG-QSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKS-AEGMLAAGFSIPGCLQDAEQIKKEHEQFQ------------------TAIEKTHASAVHVRQRAEAL 1024
D Q ++ ++ + + +QL W KK KL Q LRLF++D +++ DW+ H FL V IGR+ Q+S+ Q H F N ++N ++L+ A +L ++ ++ + VA L+ + FA ++ R +L +VLF+ +V+ + W ++ +++ T E++ + Q T+ ST LD L S IQ GE E G+ D D + + V + + + L W +K++L L LRLF+ D ++ + GE + RA ++ + + TY+ ++ LL ++ L + N E V E L+ + RR LE ++ + S +++ W+ + L S + SL AE L EQF + T + +TLL+ + ++ A N Q+ + R + + N K +C L ER+ + + W + +GD R + ++ + A +++ T +A+R E N L + +Y G + +V+ +LE + +E + E + ++ +L+Q Q FE A Q + WIK + L+ + ++ E + +EH F+ T +EK HA A +++Q E +
Sbjct: 604 DYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYS----HVEELGSWL--AELKGELASEEVAETAENA-------ERLLDQFSTQREST-------LDAL---QSTIQEGESLLRELGEQGI-----DQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREY----KREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACT--LARRTAETFLKYSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEV 1293 HSP 4 Score: 65.4698 bits (158), Expect = 9.019e-7 Identity = 40/165 (24.24%), Postives = 79/165 (47.88%), Query Frame = 0
Query: 324 YSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDER----TTVLGLSV 484
YS R N +P+ + + +L+ + + + ++L+ W KK +LDQ Q LFE+ + WI +L + +G++ +E++ L +HN F + + +L A L+E H + +++ +D+ +K+F+ +D+ LGLSV
Sbjct: 1155 YSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGLSV 1319
BLAST of triple functional domain partial vs. nr
Match: gi|1325254098|ref|XP_023330490.1| (triple functional domain protein-like isoform X3 [Eurytemora affinis] >gi|1325254103|ref|XP_023330570.1| triple functional domain protein-like isoform X3 [Eurytemora affinis]) HSP 1 Score: 1698.72 bits (4398), Expect = 0.000e+0 Identity = 826/1188 (69.53%), Postives = 971/1188 (81.73%), Query Frame = 0
Query: 78 GHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRG-STWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSS-AIQNGEHKKHES-----RHSGVPPPKK-DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRD-VKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G R D LR +DVM + E LA+L+GGRDRRGG ++T PRRERARPED+K LL YLLGVPC+DVR++GF V+IDMRG S+WTTVKPILKVLQ+ ++ IH+ IIKP+ FWQKQRT+LGSQKYKFET+ I L+SL K +DPS LT DL+G L+YDHT WIE+RC LEDF+WQS+E++DR++ MREDLNHNDFADDVNGA+K+ ++HNE K+K+ K+PVENID +GQ LLQR STCEGGNSYDSGY GRDSTASNMT NPD+Q +IP IMQ+LD VHSGQQQLLQLW++K++KLD C QLR+FEQDCEKMFDWI +R FL NYV IGR++Q++K +Q++HN FT SNNV+ NI+RIL+ A KLIE HYA+ HVR VA+RLD WK FAG LDERTTVL LSVLFHQKA+ YV++V+ WS GC++ ++DI+ LE+SIH HQ+LYE MCQ+YTEVHSTSKKLLYQLDHLVQ S+ +Q KKH S R PPPK D DY EGA HVL+VIH IL HHR LE KWH KKIKLHQRLALRLFQEDVKQVLDWL+ HGEVFLRKNVGIGR+LQK+R YQKSHETFEGVVQNTYTNAEKLLAAA ELAQTGECNAEDIYSVA ELE+HI+SFA RVE RR +L+ A+ FYSH EEL SWL+ELK EL SEEV+E+ E AERL++QFS QR+STLDA STI EG++LL EL GI E+D++GS+ SVQSTL RL +R+ELGE+W TRK+RLDLC QLRLFERDALELS+Q+E+W E+L G LPR+ V++ E R L + I+HIQSATY V QRGQ+LL++ E+SG+ LM+DSQYNG++RVQVLLEY+QER +D+EEL + RR+KLEQC+ L+ ++ DA+QV+RWI++ E ML A FSIPG L DAE +KKEHEQFQ AIEKTH+SAVHVRQRAE LLQ NHF+P VKEIAD VT RWQ LVTRAEERHKLVTASLNFYKTAEQVCSVLDSLE+EY+R+DDWL R T HD+ L+AQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYF E ++GPE KVK ILE+LLTQENRVLEYWT KKKRL+QNQQ+ LFERSA+QSLAWIKEEGDIYLS+H ++VG KEETE LL EHNSFK AR
Sbjct: 87 GARRVDGLRCLDVMKEMEEGLALLTGGRDRRGGPVLTLPQNPRRERARPEDYKKLLDYLLGVPCDDVREMGFTVVIDMRGNSSWTTVKPILKVLQDNYSTSIHTALIIKPDTFWQKQRTNLGSQKYKFETHLIGLESLHKQVDPSQLTVDLEGTLSYDHTIWIEMRCGLEDFVWQSSELLDRMEDMREDLNHNDFADDVNGAKKSMDLHNESKKKIHKVPVENIDGMGQRLLQRLSTCEGGNSYDSGYCGRDSTASNMTLNPDLQVAIPQIMQLLDQVHSGQQQLLQLWKVKRNKLDHCLQLRMFEQDCEKMFDWIFSNRDVFLGNYVEIGRNYQDAKTIQEEHNQFTAASNNVYVNISRILTAAGKLIEGGHYAAVHVRDVASRLDSTWKGFAGGLDERTTVLALSVLFHQKAETYVENVSGWSGGCEV---QGVSSDITVLETSIHHHQALYEAMCQAYTEVHSTSKKLLYQLDHLVQVSAQTVQESPQKKHSSGCPQKRKEPRPPPKSTDPAADYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYSHVEELGSWLAELKGELASEEVAETAENAERLLDQFSTQRESTLDALQSTIQEGESLLRELGEQGIDQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGD---HDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFNHPNHPE--YRGPELKVKN----------ILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTH-TQVGKNKEETEVLLQEHNSFKEKAR 1255 HSP 2 Score: 96.6709 bits (239), Expect = 3.064e-16 Identity = 89/436 (20.41%), Postives = 185/436 (42.43%), Query Frame = 0
Query: 304 GQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTKWSQGCD--MTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
GQ LLQ F + DS Y+G + +L+ + + +L +++ KL+QC L ++ D ++ WI + ++ SI S +++ L+ +H F + S+ + A L++ NH+ V+ +A + W++ +ER ++ S+ F++ A+Q +DS+ + + D + S D +TL + I++HQ E ++ T T++ L ++ ++Q H H +Y V ++ +L + W KK +L Q LF+ +Q L W++ G+++L + +G++ ++ + H +F+ + T + LL A L + G +A +I E++ +F+ R+++ R+ LE ++
Sbjct: 917 GQDLLQLFESSGINLMADSQYNGETR-----------------VQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASF-SIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKY------SNRSLQYFNHPNHP---------------EYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGL 1313 HSP 3 Score: 95.1301 bits (235), Expect = 1.050e-15 Identity = 160/724 (22.10%), Postives = 295/724 (40.75%), Query Frame = 0
Query: 339 DMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEK---LL----AAAAELAQTGECNAEDIYSVAHE-LESHISSFAQRVEERRKLLELAIA---FYSHTEELISWLSELKQELQ-SEEVSESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDS---QYNG-QSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKS-AEGMLAAGFSIPGCLQDAEQIKKEHEQFQ------------------TAIEKTHASAVHVRQRAEAL 1024
D Q ++ ++ + + +QL W KK KL Q LRLF++D +++ DW+ H FL V IGR+ Q+S+ Q H F N ++N ++L+ A +L ++ ++ + VA L+ + FA ++ R +L +VLF+ +V+ + W ++ +++ T E++ + Q T+ ST LD L S IQ GE E G+ D D + + V + + + L W +K++L L LRLF+ D ++ + GE + RA ++ + + TY+ ++ LL ++ L + N E V E L+ + RR LE ++ + S +++ W+ + L S + SL AE L EQF + T + +TLL+ + ++ A N Q+ + R + + N K +C L ER+ + + W + +GD R + ++ + A +++ T +A+R E N L + +Y G + +V+ +LE + +E + E + ++ +L+Q Q FE A Q + WIK + L+ + ++ E + +EH F+ T +EK HA A +++Q E +
Sbjct: 600 DYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYS----HVEELGSWL--AELKGELASEEVAETAENA-------ERLLDQFSTQREST-------LDAL---QSTIQEGESLLRELGEQGI-----DQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREY----KREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACT--LARRTAETFLKYSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEV 1289 HSP 4 Score: 65.4698 bits (158), Expect = 9.646e-7 Identity = 40/165 (24.24%), Postives = 79/165 (47.88%), Query Frame = 0
Query: 324 YSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDER----TTVLGLSV 484
YS R N +P+ + + +L+ + + + ++L+ W KK +LDQ Q LFE+ + WI +L + +G++ +E++ L +HN F + + +L A L+E H + +++ +D+ +K+F+ +D+ LGLSV
Sbjct: 1151 YSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGLSV 1315
BLAST of triple functional domain partial vs. nr
Match: gi|1325254069|ref|XP_023330210.1| (triple functional domain protein-like isoform X1 [Eurytemora affinis] >gi|1325254085|ref|XP_023330286.1| triple functional domain protein-like isoform X1 [Eurytemora affinis] >gi|1325254090|ref|XP_023330347.1| triple functional domain protein-like isoform X1 [Eurytemora affinis]) HSP 1 Score: 1698.72 bits (4398), Expect = 0.000e+0 Identity = 830/1211 (68.54%), Postives = 980/1211 (80.92%), Query Frame = 0
Query: 55 GPLTRAPAPSATDPVAQIRRPIGGHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRG-STWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSS-AIQNGEHKKHES-----RHSGVPPPKK-DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRD-VKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G L+R+ P + + + G R D LR +DVM + E LA+L+GGRDRRGG ++T PRRERARPED+K LL YLLGVPC+DVR++GF V+IDMRG S+WTTVKPILKVLQ+ ++ IH+ IIKP+ FWQKQRT+LGSQKYKFET+ I L+SL K +DPS LT DL+G L+YDHT WIE+RC LEDF+WQS+E++DR++ MREDLNHNDFADDVNGA+K+ ++HNE K+K+ K+PVENID +GQ LLQR STCEGGNSYDSGY GRDSTASNMT NPD+Q +IP IMQ+LD VHSGQQQLLQLW++K++KLD C QLR+FEQDCEKMFDWI +R FL NYV IGR++Q++K +Q++HN FT SNNV+ NI+RIL+ A KLIE HYA+ HVR VA+RLD WK FAG LDERTTVL LSVLFHQKA+ YV++V+ WS GC++ ++DI+ LE+SIH HQ+LYE MCQ+YTEVHSTSKKLLYQLDHLVQ S+ +Q KKH S R PPPK D DY EGA HVL+VIH IL HHR LE KWH KKIKLHQRLALRLFQEDVKQVLDWL+ HGEVFLRKNVGIGR+LQK+R YQKSHETFEGVVQNTYTNAEKLLAAA ELAQTGECNAEDIYSVA ELE+HI+SFA RVE RR +L+ A+ FYSH EEL SWL+ELK EL SEEV+E+ E AERL++QFS QR+STLDA STI EG++LL EL GI E+D++GS+ SVQSTL RL +R+ELGE+W TRK+RLDLC QLRLFERDALELS+Q+E+W E+L G LPR+ V++ E R L + I+HIQSATY V QRGQ+LL++ E+SG+ LM+DSQYNG++RVQVLLEY+QER +D+EEL + RR+KLEQC+ L+ ++ DA+QV+RWI++ E ML A FSIPG L DAE +KKEHEQFQ AIEKTH+SAVHVRQRAE LLQ NHF+P VKEIAD VT RWQ LVTRAEERHKLVTASLNFYKTAEQVCSVLDSLE+EY+R+DDWL R T HD+ L+AQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYF E ++GPE KVK ILE+LLTQENRVLEYWT KKKRL+QNQQ+ LFERSA+QSLAWIKEEGDIYLS+H ++VG KEETE LL EHNSFK AR
Sbjct: 80 GNLSRSSPP---EDLEYLNNNPDGARRVDGLRCLDVMKEMEEGLALLTGGRDRRGGPVLTLPQNPRRERARPEDYKKLLDYLLGVPCDDVREMGFTVVIDMRGNSSWTTVKPILKVLQDNYSTSIHTALIIKPDTFWQKQRTNLGSQKYKFETHLIGLESLHKQVDPSQLTVDLEGTLSYDHTIWIEMRCGLEDFVWQSSELLDRMEDMREDLNHNDFADDVNGAKKSMDLHNESKKKIHKVPVENIDGMGQRLLQRLSTCEGGNSYDSGYCGRDSTASNMTLNPDLQVAIPQIMQLLDQVHSGQQQLLQLWKVKRNKLDHCLQLRMFEQDCEKMFDWIFSNRDVFLGNYVEIGRNYQDAKTIQEEHNQFTAASNNVYVNISRILTAAGKLIEGGHYAAVHVRDVASRLDSTWKGFAGGLDERTTVLALSVLFHQKAETYVENVSGWSGGCEV---QGVSSDITVLETSIHHHQALYEAMCQAYTEVHSTSKKLLYQLDHLVQVSAQTVQESPQKKHSSGCPQKRKEPRPPPKSTDPAADYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYSHVEELGSWLAELKGELASEEVAETAENAERLLDQFSTQRESTLDALQSTIQEGESLLRELGEQGIDQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGD---HDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFNHPNHPE--YRGPELKVKN----------ILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTH-TQVGKNKEETEVLLQEHNSFKEKAR 1268 HSP 2 Score: 97.0561 bits (240), Expect = 2.361e-16 Identity = 89/436 (20.41%), Postives = 185/436 (42.43%), Query Frame = 0
Query: 304 GQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTKWSQGCD--MTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAF 733
GQ LLQ F + DS Y+G + +L+ + + +L +++ KL+QC L ++ D ++ WI + ++ SI S +++ L+ +H F + S+ + A L++ NH+ V+ +A + W++ +ER ++ S+ F++ A+Q +DS+ + + D + S D +TL + I++HQ E ++ T T++ L ++ ++Q H H +Y V ++ +L + W KK +L Q LF+ +Q L W++ G+++L + +G++ ++ + H +F+ + T + LL A L + G +A +I E++ +F+ R+++ R+ LE ++
Sbjct: 930 GQDLLQLFESSGINLMADSQYNGETR-----------------VQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASF-SIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACTLARRTAETFLKY------SNRSLQYFNHPNHP---------------EYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGL 1326 HSP 3 Score: 95.5153 bits (236), Expect = 7.308e-16 Identity = 160/724 (22.10%), Postives = 295/724 (40.75%), Query Frame = 0
Query: 339 DMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEK---LL----AAAAELAQTGECNAEDIYSVAHE-LESHISSFAQRVEERRKLLELAIA---FYSHTEELISWLSELKQELQ-SEEVSESLEGAERLI---EQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDS---QYNG-QSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKS-AEGMLAAGFSIPGCLQDAEQIKKEHEQFQ------------------TAIEKTHASAVHVRQRAEAL 1024
D Q ++ ++ + + +QL W KK KL Q LRLF++D +++ DW+ H FL V IGR+ Q+S+ Q H F N ++N ++L+ A +L ++ ++ + VA L+ + FA ++ R +L +VLF+ +V+ + W ++ +++ T E++ + Q T+ ST LD L S IQ GE E G+ D D + + V + + + L W +K++L L LRLF+ D ++ + GE + RA ++ + + TY+ ++ LL ++ L + N E V E L+ + RR LE ++ + S +++ W+ + L S + SL AE L EQF + T + +TLL+ + ++ A N Q+ + R + + N K +C L ER+ + + W + +GD R + ++ + A +++ T +A+R E N L + +Y G + +V+ +LE + +E + E + ++ +L+Q Q FE A Q + WIK + L+ + ++ E + +EH F+ T +EK HA A +++Q E +
Sbjct: 613 DYQEGAGHVLSVIHEILAHHRQLEAKWHQKKIKLHQRLALRLFQEDVKQVLDWLEKHGEVFLRKNVGIGRNLQKSRVYQKSHETFEGVVQNTYTNAEKLLAAAEELAQTGECNAEDIYSVARELENHIASFAARVEGRRHILDSAVLFYS----HVEELGSWL--AELKGELASEEVAETAENA-------ERLLDQFSTQREST-------LDAL---QSTIQEGESLLRELGEQGI-----DQEMDSTGSVASVQSTLTRLSGEREELGELWTTRKLRLDLCLQLRLFERDALELSAQFEVWGEELHTGPLPRNSVEDAERASRQLDDQISHIQSATYSVVQRGQDLLQLFESSGINLMADSQYNGETRVQVLLEYLQERGVDMEELAAVRRLKLEQCVSLTTYQSDASQVMRWIQTTESMLMASFSIPGSLHDAELLKKEHEQFQVAIEKTHSSAVHVRQRAETLLQLNHFDPAGVKEIADSVTNRWQNLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEREY----KREDDWLARHTSGDHDRATLIAQLINKHQEQKEAFLKACT--LARRTAETFLKYSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEV 1302 HSP 4 Score: 65.4698 bits (158), Expect = 9.651e-7 Identity = 40/165 (24.24%), Postives = 79/165 (47.88%), Query Frame = 0
Query: 324 YSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDER----TTVLGLSV 484
YS R N +P+ + + +L+ + + + ++L+ W KK +LDQ Q LFE+ + WI +L + +G++ +E++ L +HN F + + +L A L+E H + +++ +D+ +K+F+ +D+ LGLSV
Sbjct: 1164 YSNRSLQYFNHPNHPEYRGPELKVKNILEKLLTQENRVLEYWTNKKKRLDQNQQYVLFERSARQSLAWIKEEGDIYLSTHTQVGKNKEETEVLLQEHNSFKEKARETREKVKLLLQLADTLVEKGHAHAPNIKQWVEEVDETYKNFSSRMDQYREKLEASLGLSV 1328
BLAST of triple functional domain partial vs. nr
Match: gi|1227985877|ref|XP_021924607.1| (kalirin isoform X5 [Zootermopsis nevadensis]) HSP 1 Score: 1555.42 bits (4026), Expect = 0.000e+0 Identity = 775/1227 (63.16%), Postives = 966/1227 (78.73%), Query Frame = 0
Query: 65 ATDPVAQIRRPIGGH-----------WRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNG-EH--KKHES-----------RHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRST----------GNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
ATD + RRP H R D LRA+DVM +L+E++A+L+GGRDRRGG +++F ATPRRERA+P+D++ LLQYLL +PC++VR+ GF V++DMRGSTW+TVKPILKVLQE F +H +IIKP+NFWQKQRTSLGS KYKFETN ISL++L K+ID + LT DL+G L+YDH+ WI++R ALEDFMWQ+ +++DRLD ++EDL+ NDFADDV GA+ ++H E+K+K++K PVE+ID++GQ LLQR S + + YDSGYSGRDS AS++ NPD+QAS+P I+Q L+ + + QQ LLQLW KK KLDQC QLRLFEQDCEKMFDWI H+R FL NYV IG S+Q +K+LQ++HNHFTM S NV+ NINRIL+ A++LIESNHYA+QH+R VAARLD+ WK+FA LDERT+VL LSVLFH KA+QYV++V+ W+ C+ MS ++I LE++IHQHQ+LYE+MCQ+YTEVHSTSKKLLYQLDHLVQ + Q G EH +KH + R +G P DYSEGASHVLAVIH IL+HHRTLE KWHGKKIKLHQRLALRLFQEDVKQVLDWL NHGEVFLRKNVGIGR+LQKAR YQKSHE FE V QNTYTNAEKLL AA ELAQTGECNA++IYSVAHELESH++SFA RVE+RR+ L+LA+ FYSH +EL W+ EL+QELQ++E +++LE AERL+EQ QRDS+LDAC STIA G+TLL+EL+S G++ E+D++GS+ +V++ LDRL QR+EL E+W TRK++LDLC +LRLFERDALE+SSQ ELW+E+LQ+ +L RD ++ E LR +E ++H+Q+ T++V Q GQEL + E SG++LM+DSQY+ Q+RVQVLLE++ ERE+DLE+L +R+KLEQC+QL QF+ DANQVI WI++ E ML A F+IP CLQDAEQ+KKEHEQFQ AIEKTH SAV V+ RAEAL+ NH++P V+EIA+ VT RWQ LVT AEERHKLVTAS+NFYKTAEQVCSVLDSLE+EY+RD+DW G T S DK V+QLINKHQEQKEAFLKACTLARRTAETFLKY+ RSLQY+ QG E S + E +VK ILE+LL QENRVLE+WT +KKRL+Q QQ+ LFERSAKQ++ WI + G+IYLS+H + VG +KEETE LL EHN FK +A+
Sbjct: 23 ATDTSSSKRRPHSWHSSTQTLSRVGARRMDGLRALDVMALLQERIAVLTGGRDRRGGPVLSFPATPRRERAKPDDYRRLLQYLLAIPCDEVREHGFTVVVDMRGSTWSTVKPILKVLQEHFPGSVHIAYIIKPDNFWQKQRTSLGSHKYKFETNMISLEALPKIIDTTQLTSDLEGTLHYDHSQWIDIRLALEDFMWQAADLLDRLDDLQEDLSRNDFADDVAGAKHGIDLHCEMKKKIMKAPVEDIDHIGQRLLQRLSG-DSNSGYDSGYSGRDSEASSVVANPDLQASVPQILQNLEAIRTSQQHLLQLWHHKKLKLDQCFQLRLFEQDCEKMFDWICHNRDVFLMNYVEIGHSYQVAKELQEEHNHFTMSSRNVYVNINRILTVASRLIESNHYAAQHIRTVAARLDRTWKEFAAGLDERTSVLALSVLFHHKAEQYVENVSNWNLACE---NMSIPSEIMILETAIHQHQNLYESMCQAYTEVHSTSKKLLYQLDHLVQICN--QPGMEHTARKHVTVDGQLVYEATGRQTGNPAA------DYSEGASHVLAVIHQILNHHRTLEQKWHGKKIKLHQRLALRLFQEDVKQVLDWLANHGEVFLRKNVGIGRNLQKARVYQKSHEHFENVAQNTYTNAEKLLTAAEELAQTGECNADEIYSVAHELESHVTSFAARVEQRRQRLDLAVLFYSHDKELAGWVDELRQELQNDEAADTLEAAERLLEQCGQQRDSSLDACVSTIARGETLLQELRSAGVSAEMDSTGSVAAVEAALDRLNKQREELQELWATRKLKLDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQVLQLGQELAQAFETSGMSLMADSQYSAQTRVQVLLEFLHEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASFAIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNY-QG-ETSTRNSENRVKN----------ILEKLLCQENRVLEHWTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTH-TNVGQSKEETETLLFEHNVFKGTAK 1224 HSP 2 Score: 127.487 bits (319), Expect = 9.925e-26 Identity = 116/519 (22.35%), Postives = 224/519 (43.16%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTK-------W--------SQGCDMTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTE 738
++L L F + E+ +L++ E+L H + + D A + +HNE + + + +GQ L Q F T DS YS Q + ++++ L H + L L ++K+ KL+QC QL F+ D ++ WI + + ++ +I Q++++L+ +H F + ++ ++ A LI +NHY Q VR +A + K W+ C +ER ++ S+ F++ A+Q +DS+ + W G ++A S+ TD +T S I++HQ E ++ T T++ L +Q + + E+R + +L + +L+H W +K +L Q LF+ KQ ++W+ + GE++L + +G+S ++ H F+G + T + L+ A L + G +A I +++ F+ R+++ R LE ++ + E
Sbjct: 809 LDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQ-VLQLGQELAQAFETSGMSLMADSQYSA--------------QTRVQVLLEFL---HEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASF-AIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNYQGETSTRNSENRVKNI--------------LEKLLCQENRVLEH-------WTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTHTNVGQSKEETETLLFEHNVFKGTAKETRERVKLLIQLADSLVEKGHAHAGTIKQWVAAVDNRYKDFSSRMDKYRSQLEGSLGIQAEGE 1287
BLAST of triple functional domain partial vs. nr
Match: gi|1227985873|ref|XP_021924605.1| (triple functional domain protein isoform X3 [Zootermopsis nevadensis]) HSP 1 Score: 1555.04 bits (4025), Expect = 0.000e+0 Identity = 776/1232 (62.99%), Postives = 968/1232 (78.57%), Query Frame = 0
Query: 65 ATDPVAQIRRPIGGH-----------WRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNG-EH--KKHESR-----HSGVPPPKK-----------DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRST----------GNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
ATD + RRP H R D LRA+DVM +L+E++A+L+GGRDRRGG +++F ATPRRERA+P+D++ LLQYLL +PC++VR+ GF V++DMRGSTW+TVKPILKVLQE F +H +IIKP+NFWQKQRTSLGS KYKFETN ISL++L K+ID + LT DL+G L+YDH+ WI++R ALEDFMWQ+ +++DRLD ++EDL+ NDFADDV GA+ ++H E+K+K++K PVE+ID++GQ LLQR S + + YDSGYSGRDS AS++ NPD+QAS+P I+Q L+ + + QQ LLQLW KK KLDQC QLRLFEQDCEKMFDWI H+R FL NYV IG S+Q +K+LQ++HNHFTM S NV+ NINRIL+ A++LIESNHYA+QH+R VAARLD+ WK+FA LDERT+VL LSVLFH KA+QYV++V+ W+ C+ MS ++I LE++IHQHQ+LYE+MCQ+YTEVHSTSKKLLYQLDHLVQ + Q G EH +KH SR H+ V + + DYSEGASHVLAVIH IL+HHRTLE KWHGKKIKLHQRLALRLFQEDVKQVLDWL NHGEVFLRKNVGIGR+LQKAR YQKSHE FE V QNTYTNAEKLL AA ELAQTGECNA++IYSVAHELESH++SFA RVE+RR+ L+LA+ FYSH +EL W+ EL+QELQ++E +++LE AERL+EQ QRDS+LDAC STIA G+TLL+EL+S G++ E+D++GS+ +V++ LDRL QR+EL E+W TRK++LDLC +LRLFERDALE+SSQ ELW+E+LQ+ +L RD ++ E LR +E ++H+Q+ T++V Q GQEL + E SG++LM+DSQY+ Q+RVQVLLE++ ERE+DLE+L +R+KLEQC+QL QF+ DANQVI WI++ E ML A F+IP CLQDAEQ+KKEHEQFQ AIEKTH SAV V+ RAEAL+ NH++P V+EIA+ VT RWQ LVT AEERHKLVTAS+NFYKTAEQVCSVLDSLE+EY+RD+DW G T S DK V+QLINKHQEQKEAFLKACTLARRTAETFLKY+ RSLQY+ QG E S + E +VK ILE+LL QENRVLE+WT +KKRL+Q QQ+ LFERSAKQ++ WI + G+IYLS+H + VG +KEETE LL EHN FK +A+
Sbjct: 23 ATDTSSSKRRPHSWHSSTQTLSRVGARRMDGLRALDVMALLQERIAVLTGGRDRRGGPVLSFPATPRRERAKPDDYRRLLQYLLAIPCDEVREHGFTVVVDMRGSTWSTVKPILKVLQEHFPGSVHIAYIIKPDNFWQKQRTSLGSHKYKFETNMISLEALPKIIDTTQLTSDLEGTLHYDHSQWIDIRLALEDFMWQAADLLDRLDDLQEDLSRNDFADDVAGAKHGIDLHCEMKKKIMKAPVEDIDHIGQRLLQRLSG-DSNSGYDSGYSGRDSEASSVVANPDLQASVPQILQNLEAIRTSQQHLLQLWHHKKLKLDQCFQLRLFEQDCEKMFDWICHNRDVFLMNYVEIGHSYQVAKELQEEHNHFTMSSRNVYVNINRILTVASRLIESNHYAAQHIRTVAARLDRTWKEFAAGLDERTSVLALSVLFHHKAEQYVENVSNWNLACE---NMSIPSEIMILETAIHQHQNLYESMCQAYTEVHSTSKKLLYQLDHLVQICN--QPGMEHTARKHNSRRKPKGHTSVTVDGQLVYEATGRQTGNPAADYSEGASHVLAVIHQILNHHRTLEQKWHGKKIKLHQRLALRLFQEDVKQVLDWLANHGEVFLRKNVGIGRNLQKARVYQKSHEHFENVAQNTYTNAEKLLTAAEELAQTGECNADEIYSVAHELESHVTSFAARVEQRRQRLDLAVLFYSHDKELAGWVDELRQELQNDEAADTLEAAERLLEQCGQQRDSSLDACVSTIARGETLLQELRSAGVSAEMDSTGSVAAVEAALDRLNKQREELQELWATRKLKLDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQVLQLGQELAQAFETSGMSLMADSQYSAQTRVQVLLEFLHEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASFAIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNY-QG-ETSTRNSENRVKN----------ILEKLLCQENRVLEHWTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTH-TNVGQSKEETETLLFEHNVFKGTAK 1235 HSP 2 Score: 127.872 bits (320), Expect = 8.769e-26 Identity = 116/519 (22.35%), Postives = 224/519 (43.16%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTK-------W--------SQGCDMTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTE 738
++L L F + E+ +L++ E+L H + + D A + +HNE + + + +GQ L Q F T DS YS Q + ++++ L H + L L ++K+ KL+QC QL F+ D ++ WI + + ++ +I Q++++L+ +H F + ++ ++ A LI +NHY Q VR +A + K W+ C +ER ++ S+ F++ A+Q +DS+ + W G ++A S+ TD +T S I++HQ E ++ T T++ L +Q + + E+R + +L + +L+H W +K +L Q LF+ KQ ++W+ + GE++L + +G+S ++ H F+G + T + L+ A L + G +A I +++ F+ R+++ R LE ++ + E
Sbjct: 820 LDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQ-VLQLGQELAQAFETSGMSLMADSQYSA--------------QTRVQVLLEFL---HEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASF-AIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNYQGETSTRNSENRVKNI--------------LEKLLCQENRVLEH-------WTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTHTNVGQSKEETETLLFEHNVFKGTAKETRERVKLLIQLADSLVEKGHAHAGTIKQWVAAVDNRYKDFSSRMDKYRSQLEGSLGIQAEGE 1298
BLAST of triple functional domain partial vs. nr
Match: gi|1339092584|ref|XP_023707504.1| (kalirin isoform X2 [Cryptotermes secundus]) HSP 1 Score: 1551.18 bits (4015), Expect = 0.000e+0 Identity = 765/1203 (63.59%), Postives = 959/1203 (79.72%), Query Frame = 0
Query: 78 GHWRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEH---KKHES-----------RHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRST----------GNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
G R D LRA+DVM +L+E+LA+L+GGRDRRGG +++F ATPRRERA+P+D++ LLQYLL +PC++VR+ GF V+IDMRG+TW+TVKPILKVLQE F +H +IIKP+NFWQKQRTSLGS KYKFETN ISL++L K+ID + LT DL+G L+YDH+ WI++R ALEDF+WQ+ +++DRLD ++EDL+ NDFADDV GA+ ++H E+K+K++K PVE+ID++GQ LLQR S + + YDSGYSGRDS AS++ NPD+QAS+P I+Q L+ + + QQ LLQLW KK KLDQC QLRLFEQDCEKMFDWI H+R FL NYV IG S+Q +K+LQ++HNHFTM S NV+ NINRIL+ A++LIESNHYA+QH+R VAARLD+ WK+FA LDERT+VL LSVLFH KA+QYV++V+ W Q C+ MS ++I LE++IHQHQ+LYE+MCQ+YTEVHSTSKKLLYQLDHLVQ + Q G +KH + R +G P DYSEGASHVLAVIH IL+HHRTLE KWH KKIKLHQRLALRLFQEDVKQVLDWL NHGEVFLRKN+GIGR+LQKAR YQKSHE FE V QNTYTNAEKLL AA ELAQTGECNAE+IYSVAHELE+H++SFA RVE+RR+ L+LA+ FY+ +EL SW+ EL+QELQ++E +++LE ERL+EQ S QRDS+LDAC STIA G TLL+EL+S G++ E+D++GS+ +V++ LDRL QR+EL E+W+TRK++LDLC +LRLFERDALE+SSQ ELW+E+LQ+ +L RD ++ E LR SE ++H+Q+ T++V Q+GQEL + E SG+++M+DSQY+ Q+RVQVLLE++ ERE+DLE+L +R+KLEQC+QL QF+ DANQVI WI++ E ML A F+IP CLQDAEQ+KKEHEQFQ AIEKTH SAV V+ RAEAL+ NH++P V+EIA+ VT RWQ LVT AEERHKLVTASLNFYKTAEQVCSVLDSLE+EY+RD+DW G T S D+ A V+QLINKHQEQKEAFLKACTLARRTAETFLKY+ RSLQY+ QG E S + E +VK ILE+LL+QEN+VLE+WT +KKRL+Q QQ+ LFERSAKQ++ WI + G+IYLS+H + VG +KEETE LL EHN FK +A+
Sbjct: 48 GARRMDGLRALDVMALLQERLAVLTGGRDRRGGPVLSFPATPRRERAKPDDYRRLLQYLLAIPCDEVREQGFTVVIDMRGATWSTVKPILKVLQEHFPGSVHIAYIIKPDNFWQKQRTSLGSHKYKFETNMISLEALPKIIDTTQLTSDLEGTLHYDHSQWIDMRLALEDFVWQAADLLDRLDDLQEDLSRNDFADDVAGAKHGIDLHAEMKKKIMKAPVEDIDHIGQRLLQRLSG-DSNSGYDSGYSGRDSEASSVVANPDLQASVPQILQNLEAIRTSQQHLLQLWHHKKLKLDQCFQLRLFEQDCEKMFDWICHNRDVFLMNYVEIGHSYQVAKELQEEHNHFTMSSRNVYVNINRILTVASRLIESNHYAAQHIRTVAARLDRTWKEFAAGLDERTSVLALSVLFHHKAEQYVENVSNWKQACE---NMSIPSEILILETAIHQHQNLYESMCQAYTEVHSTSKKLLYQLDHLVQICN--QPGMEQTTRKHVTVDGQVVYEATGRQTGNP------AADYSEGASHVLAVIHQILNHHRTLEQKWHAKKIKLHQRLALRLFQEDVKQVLDWLANHGEVFLRKNIGIGRNLQKARVYQKSHEHFENVAQNTYTNAEKLLTAAEELAQTGECNAEEIYSVAHELETHVTSFAARVEQRRQRLDLAVLFYTQDKELTSWVDELRQELQNDEAADTLEATERLLEQCSQQRDSSLDACVSTIARGDTLLQELRSAGVSAEMDSTGSVAAVEAALDRLNKQREELEELWSTRKLKLDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHSESVSHMQNTTFQVLQQGQELAQAFETSGMSVMADSQYSAQTRVQVLLEFLHEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLTASFAIPSCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPADKMGAVGSNTSSSGTDRAAAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNY-QG-ETSTRNSENRVKN----------ILEKLLSQENKVLEHWTQRKKRLDQCQQYVLFERSAKQAIEWIHDTGEIYLSTH-TNVGQSKEETETLLLEHNVFKGTAK 1225 HSP 2 Score: 125.946 bits (315), Expect = 3.587e-25 Identity = 112/519 (21.58%), Postives = 217/519 (41.81%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTK-------WSQGCDMTSAM--------SAATD-ISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTE 738
++L L F + E+ +L++ E+L H + + D A + +H+E + + + GQ L Q F T DS YS Q + ++++ L H + L L ++K+ KL+QC QL F+ D ++ WI + ++ +I Q++++L+ +H F + ++ ++ A LI +NHY Q VR +A + K W+ C +ER ++ S+ F++ A+Q +DS+ + W G M S+ TD + + I++HQ E ++ T T++ L +Q + + E+R V ++ +L + W +K +L Q LF+ KQ ++W+ + GE++L + +G+S ++ H F+G + T + L+ A L + G +A I +++ F+ R+++ R LE ++ +E
Sbjct: 810 LDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHSESVSHMQNTTFQVLQQ-GQELAQAFETSGMSVMADSQYSA--------------QTRVQVLLEFL---HEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLTASF-AIPSCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASLNFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPADKMGAVGSNTSSSGTDRAAAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNYQGETSTRNSENR---------------------VKNILEKLLSQENKVLEHWTQRKKRLDQCQQYVLFERSAKQAIEWIHDTGEIYLSTHTNVGQSKEETETLLLEHNVFKGTAKETRERVKLLIQLADSLVEKGHAHASAIKQWVAAVDNRYKGFSSRMDKYRSQLEGSLGIQVESE 1288
BLAST of triple functional domain partial vs. nr
Match: gi|646711972|gb|KDR16954.1| (Triple functional domain protein, partial [Zootermopsis nevadensis]) HSP 1 Score: 1545.79 bits (4001), Expect = 0.000e+0 Identity = 762/1194 (63.82%), Postives = 953/1194 (79.82%), Query Frame = 0
Query: 83 DALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEV-----------------HSTSKKLLYQLDHLVQTSSAIQNGEHKKHESR-HSGVPPPKK--DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRSTGNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
D LRA+DVM +L+E++A+L+GGRDRRGG +++F ATPRRERA+P+D++ LLQYLL +PC++VR+ GF V++DMRGSTW+TVKPILKVLQE F +H +IIKP+NFWQKQRTSLGS KYKFETN ISL++L K+ID + LT DL+G L+YDH+ WI++R ALEDFMWQ+ +++DRLD ++EDL+ NDFADDV GA+ ++H E+K+K++K PVE+ID++GQ LLQR S + + YDSGYSGRDS AS++ NPD+QAS+P I+Q L+ + + QQ LLQLW KK KLDQC QLRLFEQDCEKMFDWI H+R FL NYV IG S+Q +K+LQ++HNHFTM S NV+ NINRIL+ A++LIESNHYA+QH+R VAARLD+ WK+FA LDERT+VL LSVLFH KA+QYV++V+ W+ C+ MS ++I LE++IHQHQ+LYE+MCQ+YTEV HSTSKKLLYQLDHLVQ I N +H +R H G ++ + DYSEGASHVLAVIH IL+HHRTLE KWHGKKIKLHQRLALRLFQEDVKQVLDWL NHGEVFLRKNVGIGR+LQKAR YQKSHE FE V QNTYTNAEKLL AA ELAQTGECNA++IYSVAHELESH++SFA RVE+RR+ L+LA+ FYSH +EL W+ EL+QELQ++E +++LE AERL+EQ QRDS+LDAC STIA G+TLL+EL+S G++ E+D++GS+ +V++ LDRL QR+EL E+W TRK++LDLC +LRLFERDALE+SSQ ELW+E+LQ+ +L RD ++ E LR +E ++H+Q+ T++V Q GQEL + E SG++LM+DSQY+ Q+RVQVLLE++ ERE+DLE+L +R+KLEQC+QL QF+ DANQVI WI++ E ML A F+IP CLQDAEQ+KKEHEQFQ AIEKTH SAV V+ RAEAL+ NH++P V+EIA+ VT RWQ LVT AEERHKLVTAS+NFYKTAEQVCSVLDSLE+EY+RD+DW G DK V+QLINKHQEQKEAFLKACTLARRTAETFLKY+ RSLQY+ QG E S + E +VK ILE+LL QENRVLE+WT +KKRL+Q QQ+ LFERSAKQ++ WI + G+IYLS+H + VG +KEETE LL EHN FK +A+
Sbjct: 2 DGLRALDVMALLQERIAVLTGGRDRRGGPVLSFPATPRRERAKPDDYRRLLQYLLAIPCDEVREHGFTVVVDMRGSTWSTVKPILKVLQEHFPGSVHIAYIIKPDNFWQKQRTSLGSHKYKFETNMISLEALPKIIDTTQLTSDLEGTLHYDHSQWIDIRLALEDFMWQAADLLDRLDDLQEDLSRNDFADDVAGAKHGIDLHCEMKKKIMKAPVEDIDHIGQRLLQRLSG-DSNSGYDSGYSGRDSEASSVVANPDLQASVPQILQNLEAIRTSQQHLLQLWHHKKLKLDQCFQLRLFEQDCEKMFDWICHNRDVFLMNYVEIGHSYQVAKELQEEHNHFTMSSRNVYVNINRILTVASRLIESNHYAAQHIRTVAARLDRTWKEFAAGLDERTSVLALSVLFHHKAEQYVENVSNWNLACE---NMSIPSEIMILETAIHQHQNLYESMCQAYTEVYNSYQALLNGLDIMVQVHSTSKKLLYQLDHLVQ----ICNQPGMEHTARKHVGKSFCRQTGNPAADYSEGASHVLAVIHQILNHHRTLEQKWHGKKIKLHQRLALRLFQEDVKQVLDWLANHGEVFLRKNVGIGRNLQKARVYQKSHEHFENVAQNTYTNAEKLLTAAEELAQTGECNADEIYSVAHELESHVTSFAARVEQRRQRLDLAVLFYSHDKELAGWVDELRQELQNDEAADTLEAAERLLEQCGQQRDSSLDACVSTIARGETLLQELRSAGVSAEMDSTGSVAAVEAALDRLNKQREELQELWATRKLKLDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQVLQLGQELAQAFETSGMSLMADSQYSAQTRVQVLLEFLHEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASFAIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCG----------DKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNY-QG-ETSTRNSENRVKN----------ILEKLLCQENRVLEHWTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTH-TNVGQSKEETETLLFEHNVFKGTAK 1164 HSP 2 Score: 125.561 bits (314), Expect = 3.480e-25 Identity = 113/503 (22.47%), Postives = 219/503 (43.54%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTE 738
++L L F + E+ +L++ E+L H + + D A + +HNE + + + +GQ L Q F T DS YS Q + ++++ L H + L L ++K+ KL+QC QL F+ D ++ WI + + ++ +I Q++++L+ +H F + ++ ++ A LI +NHY Q VR +A + K W+ C +ER ++ S+ F++ A+Q +DS+ + + D AT +S L I++HQ E ++ T T++ L +Q + + E+R + +L + +L+H W +K +L Q LF+ KQ ++W+ + GE++L + +G+S ++ H F+G + T + L+ A L + G +A I +++ F+ R+++ R LE ++ + E
Sbjct: 769 LDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQ-VLQLGQELAQAFETSGMSLMADSQYSA--------------QTRVQVLLEFL---HEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASF-AIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKR-DEDWCGDKATAVSQL---INKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNYQGETSTRNSENRVKNILEK--------------LLCQENRVLEH-------WTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTHTNVGQSKEETETLLFEHNVFKGTAKETRERVKLLIQLADSLVEKGHAHAGTIKQWVAAVDNRYKDFSSRMDKYRSQLEGSLGIQAEGE 1227
BLAST of triple functional domain partial vs. nr
Match: gi|1227985869|ref|XP_021924603.1| (triple functional domain protein isoform X1 [Zootermopsis nevadensis]) HSP 1 Score: 1538.47 bits (3982), Expect = 0.000e+0 Identity = 776/1268 (61.20%), Postives = 968/1268 (76.34%), Query Frame = 0
Query: 65 ATDPVAQIRRPIGGH-----------WRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEV------------------------------------HSTSKKLLYQLDHLVQTSSAIQNG-EH--KKHESR-----HSGVPPPKK-----------DSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRST----------GNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
ATD + RRP H R D LRA+DVM +L+E++A+L+GGRDRRGG +++F ATPRRERA+P+D++ LLQYLL +PC++VR+ GF V++DMRGSTW+TVKPILKVLQE F +H +IIKP+NFWQKQRTSLGS KYKFETN ISL++L K+ID + LT DL+G L+YDH+ WI++R ALEDFMWQ+ +++DRLD ++EDL+ NDFADDV GA+ ++H E+K+K++K PVE+ID++GQ LLQR S + + YDSGYSGRDS AS++ NPD+QAS+P I+Q L+ + + QQ LLQLW KK KLDQC QLRLFEQDCEKMFDWI H+R FL NYV IG S+Q +K+LQ++HNHFTM S NV+ NINRIL+ A++LIESNHYA+QH+R VAARLD+ WK+FA LDERT+VL LSVLFH KA+QYV++V+ W+ C+ MS ++I LE++IHQHQ+LYE+MCQ+YTEV HSTSKKLLYQLDHLVQ + Q G EH +KH SR H+ V + + DYSEGASHVLAVIH IL+HHRTLE KWHGKKIKLHQRLALRLFQEDVKQVLDWL NHGEVFLRKNVGIGR+LQKAR YQKSHE FE V QNTYTNAEKLL AA ELAQTGECNA++IYSVAHELESH++SFA RVE+RR+ L+LA+ FYSH +EL W+ EL+QELQ++E +++LE AERL+EQ QRDS+LDAC STIA G+TLL+EL+S G++ E+D++GS+ +V++ LDRL QR+EL E+W TRK++LDLC +LRLFERDALE+SSQ ELW+E+LQ+ +L RD ++ E LR +E ++H+Q+ T++V Q GQEL + E SG++LM+DSQY+ Q+RVQVLLE++ ERE+DLE+L +R+KLEQC+QL QF+ DANQVI WI++ E ML A F+IP CLQDAEQ+KKEHEQFQ AIEKTH SAV V+ RAEAL+ NH++P V+EIA+ VT RWQ LVT AEERHKLVTAS+NFYKTAEQVCSVLDSLE+EY+RD+DW G T S DK V+QLINKHQEQKEAFLKACTLARRTAETFLKY+ RSLQY+ QG E S + E +VK ILE+LL QENRVLE+WT +KKRL+Q QQ+ LFERSAKQ++ WI + G+IYLS+H + VG +KEETE LL EHN FK +A+
Sbjct: 23 ATDTSSSKRRPHSWHSSTQTLSRVGARRMDGLRALDVMALLQERIAVLTGGRDRRGGPVLSFPATPRRERAKPDDYRRLLQYLLAIPCDEVREHGFTVVVDMRGSTWSTVKPILKVLQEHFPGSVHIAYIIKPDNFWQKQRTSLGSHKYKFETNMISLEALPKIIDTTQLTSDLEGTLHYDHSQWIDIRLALEDFMWQAADLLDRLDDLQEDLSRNDFADDVAGAKHGIDLHCEMKKKIMKAPVEDIDHIGQRLLQRLSG-DSNSGYDSGYSGRDSEASSVVANPDLQASVPQILQNLEAIRTSQQHLLQLWHHKKLKLDQCFQLRLFEQDCEKMFDWICHNRDVFLMNYVEIGHSYQVAKELQEEHNHFTMSSRNVYVNINRILTVASRLIESNHYAAQHIRTVAARLDRTWKEFAAGLDERTSVLALSVLFHHKAEQYVENVSNWNLACE---NMSIPSEIMILETAIHQHQNLYESMCQAYTEVYNSYQALLNGLDIMVQVCHCFPVMGTARDCIFSNDVHSTSKKLLYQLDHLVQICN--QPGMEHTARKHNSRRKPKGHTSVTVDGQLVYEATGRQTGNPAADYSEGASHVLAVIHQILNHHRTLEQKWHGKKIKLHQRLALRLFQEDVKQVLDWLANHGEVFLRKNVGIGRNLQKARVYQKSHEHFENVAQNTYTNAEKLLTAAEELAQTGECNADEIYSVAHELESHVTSFAARVEQRRQRLDLAVLFYSHDKELAGWVDELRQELQNDEAADTLEAAERLLEQCGQQRDSSLDACVSTIARGETLLQELRSAGVSAEMDSTGSVAAVEAALDRLNKQREELQELWATRKLKLDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQVLQLGQELAQAFETSGMSLMADSQYSAQTRVQVLLEFLHEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASFAIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNY-QG-ETSTRNSENRVKN----------ILEKLLCQENRVLEHWTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTH-TNVGQSKEETETLLFEHNVFKGTAK 1271 HSP 2 Score: 127.872 bits (320), Expect = 9.648e-26 Identity = 116/519 (22.35%), Postives = 224/519 (43.16%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTK-------W--------SQGCDMTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTE 738
++L L F + E+ +L++ E+L H + + D A + +HNE + + + +GQ L Q F T DS YS Q + ++++ L H + L L ++K+ KL+QC QL F+ D ++ WI + + ++ +I Q++++L+ +H F + ++ ++ A LI +NHY Q VR +A + K W+ C +ER ++ S+ F++ A+Q +DS+ + W G ++A S+ TD +T S I++HQ E ++ T T++ L +Q + + E+R + +L + +L+H W +K +L Q LF+ KQ ++W+ + GE++L + +G+S ++ H F+G + T + L+ A L + G +A I +++ F+ R+++ R LE ++ + E
Sbjct: 856 LDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQ-VLQLGQELAQAFETSGMSLMADSQYSA--------------QTRVQVLLEFL---HEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASF-AIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNYQGETSTRNSENRVKNI--------------LEKLLCQENRVLEH-------WTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTHTNVGQSKEETETLLFEHNVFKGTAKETRERVKLLIQLADSLVEKGHAHAGTIKQWVAAVDNRYKDFSSRMDKYRSQLEGSLGIQAEGE 1334
BLAST of triple functional domain partial vs. nr
Match: gi|1227985871|ref|XP_021924604.1| (kalirin isoform X2 [Zootermopsis nevadensis]) HSP 1 Score: 1536.55 bits (3977), Expect = 0.000e+0 Identity = 775/1263 (61.36%), Postives = 966/1263 (76.48%), Query Frame = 0
Query: 65 ATDPVAQIRRPIGGH-----------WRPDALRAVDVMGVLREQLAILSGGRDRRGGAIVTFQATPRRERARPEDFKHLLQYLLGVPCEDVRDLGFAVIIDMRGSTWTTVKPILKVLQEFFAHQIHSVHIIKPENFWQKQRTSLGSQKYKFETNPISLDSLMKVIDPSNLTQDLDGALNYDHTTWIELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQYVDSVTKWSQGCDMTSAMSAATDISTLESSIHQHQSLYETMCQSYTEV------------------------------------HSTSKKLLYQLDHLVQTSSAIQNG-EH--KKHES-----------RHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTEELISWLSELKQELQSEEVSESLEGAERLIEQFSNQRDSTLDACASTIAEGKTLLEELKSNGITMELDASGSMNSVQSTLDRLGAQRDELGEVWNTRKMRLDLCHQLRLFERDALELSSQYELWAEQLQNGDLPRDVKETESQLRNLSEHIAHIQSATYEVAQRGQELLKILENSGLTLMSDSQYNGQSRVQVLLEYIQEREIDLEELGSQRRIKLEQCIQLSQFEGDANQVIRWIKSAEGMLAAGFSIPGCLQDAEQIKKEHEQFQTAIEKTHASAVHVRQRAEALLQNNHFNPGRVKEIADGVTTRWQGLVTRAEERHKLVTASLNFYKTAEQVCSVLDSLEKEYRRDDDWLGRST----------GNSDHHHDKVALVAQLINKHQEQKEAFLKACTLARRTAETFLKYSNRSLQYFTLTQGSEASFKGPEAKVKGNRLMIDCPRIILERLLTQENRVLEYWTTKKKRLEQNQQFCLFERSAKQSLAWIKEEGDIYLSSHRSRVGTTKEETEALLAEHNSFKSSAR 1256
ATD + RRP H R D LRA+DVM +L+E++A+L+GGRDRRGG +++F ATPRRERA+P+D++ LLQYLL +PC++VR+ GF V++DMRGSTW+TVKPILKVLQE F +H +IIKP+NFWQKQRTSLGS KYKFETN ISL++L K+ID + LT DL+G L+YDH+ WI++R ALEDFMWQ+ +++DRLD ++EDL+ NDFADDV GA+ ++H E+K+K++K PVE+ID++GQ LLQR S + + YDSGYSGRDS AS++ NPD+QAS+P I+Q L+ + + QQ LLQLW KK KLDQC QLRLFEQDCEKMFDWI H+R FL NYV IG S+Q +K+LQ++HNHFTM S NV+ NINRIL+ A++LIESNHYA+QH+R VAARLD+ WK+FA LDERT+VL LSVLFH KA+QYV++V+ W+ C+ MS ++I LE++IHQHQ+LYE+MCQ+YTEV HSTSKKLLYQLDHLVQ + Q G EH +KH + R +G P DYSEGASHVLAVIH IL+HHRTLE KWHGKKIKLHQRLALRLFQEDVKQVLDWL NHGEVFLRKNVGIGR+LQKAR YQKSHE FE V QNTYTNAEKLL AA ELAQTGECNA++IYSVAHELESH++SFA RVE+RR+ L+LA+ FYSH +EL W+ EL+QELQ++E +++LE AERL+EQ QRDS+LDAC STIA G+TLL+EL+S G++ E+D++GS+ +V++ LDRL QR+EL E+W TRK++LDLC +LRLFERDALE+SSQ ELW+E+LQ+ +L RD ++ E LR +E ++H+Q+ T++V Q GQEL + E SG++LM+DSQY+ Q+RVQVLLE++ ERE+DLE+L +R+KLEQC+QL QF+ DANQVI WI++ E ML A F+IP CLQDAEQ+KKEHEQFQ AIEKTH SAV V+ RAEAL+ NH++P V+EIA+ VT RWQ LVT AEERHKLVTAS+NFYKTAEQVCSVLDSLE+EY+RD+DW G T S DK V+QLINKHQEQKEAFLKACTLARRTAETFLKY+ RSLQY+ QG E S + E +VK ILE+LL QENRVLE+WT +KKRL+Q QQ+ LFERSAKQ++ WI + G+IYLS+H + VG +KEETE LL EHN FK +A+
Sbjct: 23 ATDTSSSKRRPHSWHSSTQTLSRVGARRMDGLRALDVMALLQERIAVLTGGRDRRGGPVLSFPATPRRERAKPDDYRRLLQYLLAIPCDEVREHGFTVVVDMRGSTWSTVKPILKVLQEHFPGSVHIAYIIKPDNFWQKQRTSLGSHKYKFETNMISLEALPKIIDTTQLTSDLEGTLHYDHSQWIDIRLALEDFMWQAADLLDRLDDLQEDLSRNDFADDVAGAKHGIDLHCEMKKKIMKAPVEDIDHIGQRLLQRLSG-DSNSGYDSGYSGRDSEASSVVANPDLQASVPQILQNLEAIRTSQQHLLQLWHHKKLKLDQCFQLRLFEQDCEKMFDWICHNRDVFLMNYVEIGHSYQVAKELQEEHNHFTMSSRNVYVNINRILTVASRLIESNHYAAQHIRTVAARLDRTWKEFAAGLDERTSVLALSVLFHHKAEQYVENVSNWNLACE---NMSIPSEIMILETAIHQHQNLYESMCQAYTEVYNSYQALLNGLDIMVQVCHCFPVMGTARDCIFSNDVHSTSKKLLYQLDHLVQICN--QPGMEHTARKHVTVDGQLVYEATGRQTGNPAA------DYSEGASHVLAVIHQILNHHRTLEQKWHGKKIKLHQRLALRLFQEDVKQVLDWLANHGEVFLRKNVGIGRNLQKARVYQKSHEHFENVAQNTYTNAEKLLTAAEELAQTGECNADEIYSVAHELESHVTSFAARVEQRRQRLDLAVLFYSHDKELAGWVDELRQELQNDEAADTLEAAERLLEQCGQQRDSSLDACVSTIARGETLLQELRSAGVSAEMDSTGSVAAVEAALDRLNKQREELQELWATRKLKLDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQVLQLGQELAQAFETSGMSLMADSQYSAQTRVQVLLEFLHEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASFAIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNY-QG-ETSTRNSENRVKN----------ILEKLLCQENRVLEHWTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTH-TNVGQSKEETETLLFEHNVFKGTAK 1260 HSP 2 Score: 127.487 bits (319), Expect = 1.230e-25 Identity = 116/519 (22.35%), Postives = 224/519 (43.16%), Query Frame = 0
Query: 239 IELRCALEDFMWQSTEMMDRLDVMREDLNHNDFADDVNGARKATEVHNEVKRKVLKLPVENIDNVGQLLLQRFSTCEGGNSYDSGYSGRDSTASNMTCNPDMQASIPIIMQMLDTVHSGQQQLLQLWQIKKSKLDQCHQLRLFEQDCEKMFDWIHHHRCQFLHNYVSIGRSFQESKKLQDDHNHFTMGSNNVFSNINRILSDAAKLIESNHYASQHVRGVAARLDKNWKDFAGCLDERTTVLGLSVLFHQKAQQY---VDSVTK-------W--------SQGCDMTSAMSAATDISTLESS-IHQHQSLYETMCQSYTEVHSTSKKLLYQLDHLVQTSSAIQNGEHKKHESRHSGVPPPKKDSGEDYSEGASHVLAVIHNILDHHRTLETKWHGKKIKLHQRLALRLFQEDVKQVLDWLQNHGEVFLRKNVGIGRSLQKARAYQKSHETFEGVVQNTYTNAEKLLAAAAELAQTGECNAEDIYSVAHELESHISSFAQRVEERRKLLELAIAFYSHTE 738
++L L F + E+ +L++ E+L H + + D A + +HNE + + + +GQ L Q F T DS YS Q + ++++ L H + L L ++K+ KL+QC QL F+ D ++ WI + + ++ +I Q++++L+ +H F + ++ ++ A LI +NHY Q VR +A + K W+ C +ER ++ S+ F++ A+Q +DS+ + W G ++A S+ TD +T S I++HQ E ++ T T++ L +Q + + E+R + +L + +L+H W +K +L Q LF+ KQ ++W+ + GE++L + +G+S ++ H F+G + T + L+ A L + G +A I +++ F+ R+++ R LE ++ + E
Sbjct: 845 LDLCLRLRLFERDALEVSSQLELWSEELQHTELSRDTQKAEQLLRLHNESVSHMQNTTFQ-VLQLGQELAQAFETSGMSLMADSQYSA--------------QTRVQVLLEFL---HEREMDLEDLAEMKRVKLEQCVQLCQFQNDANQVISWIRNGEAMLMASF-AIPNCLQDAEQLKKEHEQFQVAIEKTHTSAVQVKHRAEALISANHYDPQSVREIAEEVTKRWQQLVTCAEERHKLVTASINFYKTAEQVCSVLDSLEREYKRDEDWCGGGTPAEKMGALGSNASSSGTDKATAVSQLINKHQEQKEAFLKACTLARRTAETFLKYTGRSLQYYNYQGETSTRNSENRVKNI--------------LEKLLCQENRVLEH-------WTQRKKRLDQCQQYILFERSAKQAIEWIHDTGEIYLSTHTNVGQSKEETETLLFEHNVFKGTAKETRERVKLLIQLADSLVEKGHAHAGTIKQWVAAVDNRYKDFSSRMDKYRSQLEGSLGIQAEGE 1323 The following BLAST results are available for this feature:
BLAST of triple functional domain partial vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 3
BLAST of triple functional domain partial vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of triple functional domain partial vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold462_size163801:121473..141536- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold462_size163801-snap-gene-0.52 ID=maker-scaffold462_size163801-snap-gene-0.52|Name=triple functional domain partial|organism=Tigriopus kingsejongensis|type=gene|length=20064bp|location=Sequence derived from alignment at scaffold462_size163801:121473..141536- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'triple functional domain partial' has the following synonyms
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