BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 867.07 bits (2239), Expect = 0.000e+0
Identity = 446/869 (51.32%), Postives = 598/869 (68.81%), Query Frame = 0

Query:  112 LPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRT-EDQP-EARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYV------RAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLEN--GSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRL 970
            LP+S++ILLE+ +R  +   V + D++ I+NW+     N EI F P RV++QDFTGVPAIVD AAMRDAV KLGGD  KINP  P +LVIDHS+ VD+    +AL KN +LEF+RN ER+ FL+WG S+F N  ++PP +GIVHQVN+EYLAR  FT  +      +PD++VGTDSHTTM+NGLGV+GWGVGGIEAEA MLGQ I+M++P+VVG+++ G       +TD+VLT+T+ LR +GVVG+FVEFYG G+ HL +ADRATI+NM PEYGAT G FP+D  ++ YLRLT RE  +++ ++EY     + R  ED+  +  ++  + LD+GTVV S +GPKRPQDR+A+TDM     + +      + +A   + K   V       I  D   + L  G+V IAAITSCTNTSNP V++ +GLLA+ AV KGLTV P++KTSL+PGS VVT YL+ + ++  LD LGF +VGYG  TCIGNSGPL + +      GDL  + VLSGNRNFEGRI+   + N+LASPPLV+AYA+AG + +D   + LG D     ++L+DIWP++ EIQ V    I  +MF+  YS V  G+ERW ++   +   Y W+ +STYI++PP+F  MS++  + K IKNA  L  LG S T DHISPAG    + PA +YL  + ++ ++FNSYGSRRGN  +M RGTFAN+RL N+   G  G  T +      M ++DA+M+Y++    LIV+AGK+YG GSSRDWAAKG  LLGV+AV+ ESYERIHRSNLVGMG++PLQ+ EGES  S G +GKE +S+E   N    + +D+ V    G  IK  +  VR DT  E  YY+HGGIL+Y++R++
Sbjct:   34 LPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRGKNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTD----LVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPI-----IAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDG-SIKSFKATVRFDTELELTYYRHGGILNYMIRKV 892          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 710.294 bits (1832), Expect = 0.000e+0
Identity = 392/898 (43.65%), Postives = 574/898 (63.92%), Query Frame = 0

Query:  112 LPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQ-------------------------------ALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPE-ARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSE--KTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGP-LPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLG-QDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITES-DRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSH-GLTGKEIYSIEGLENGSRH--VDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRR 969
            LP+SL++LLE  +R        +++ Q  + W       D       RV++QDFTGVPA++DLAA+R++V K GG    ++ L PA+LV+DH V VDY    Q                               AL  N   EFERN ER+ FL+W  SAF N  V+PP TG++HQVN+EYLAR V  +   G V  +PD++VGTD+HTTM+NGLGVLGWGVG ++A+A M G  +T+  P+VVG ++KG + + +T+TDLVL +T+ LR  GV G FVEF+G  L  L +ADRATI+NM PEYGA  G FPIDE T+ YL  +GR+   IE V++Y     MFR+ D+ E  R+ + +E+D+  ++P ++GPKR +D++++ +++   K+A +++   K      G      A  +  DG  FT+  G V +AAI+SCTNTSNP V++ +GLLA+ AV  GL+V   VKTSLAPGS VVT YL+++ +M  L  LGF++VGYGC+TC  NSGP LP++I  A++ G+L    +LSGNRNFEGR+  DI+ N+LASP +V+AYA+AG I +++ ++PLG     G  +FLK+IWPS++EIQE+    +   ++++ YS V  G++ W ++ +  S D++ W+SDSTYI+ P Y   +    S+ +  KN R L  LGD+IT+DHISPAGS    SPA +YL+  G+A +++NSYG+RRGN+ VM+RGTF+N+RL+N L     G +T + P +   +I++AS +Y+ +GV L  I GK +G G+SRDWA +G  LLGV+A++  S++  +RSNLV  G+LPLQ     + +S+  L G E  SI+  +N +    +D+VV    GK  +  + +VR+D   E D +  GG++  ++ +
Sbjct:   40 LPYSLRVLLECAVR-------KRSETQGQV-W------KDVYERILRRVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQ---GGVL-FPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQ--GHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYIKEIG---SNARNWKNMRCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLL-NKVGPYTLHLPSEVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQI----TSISYESLKGTECLSIDIRDNITPKCLIDVVV----GKAERSFKAVVRVDNDYETDVFHAGGVIAQIVSK 905          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 129.028 bits (323), Expect = 2.035e-30
Identity = 147/526 (27.95%), Postives = 225/526 (42.78%), Query Frame = 0

Query:  153 IAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTL-VGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMN-FLASPPLVVAYALAGNIQVDMHKD----PLGQDQQGNDVFLKDIWPSQ 672
            +   P RV MQD T   A+  L  +   + K+           P+ +  DH +     G     +K+     + NAE Y FL    + +       P +GI+HQ+ +E  A              +P  L VGTDSHT    GLG L  GVGG +A   M      +  P+V+G KL GK+    +  D++L V  +L   G  G  VE++GEG+  +      TI NM  E GAT  +FP +     YL  T R  D   L  +Y   + +  + D    +Y   +E+D+ T+ P + GP  P     + ++ EK                            ++G    ++DG      I SCTN+S   +   + ++ + A+AKG+  K      + PGS+ +   +E+  L   + + G  V+   C  CIG      + I    +N     + V S NRNF GR  A+   + F+ SP + VA +LAG++  D   D    P G + + N  F  D  PSQ
Sbjct:  672 LRLRPDRVAMQDATAQMAM--LQFISSGLPKVA---------VPSTIHCDHLIQAQIEG-----KKDLARAKDLNAEVYNFLSTSGAKY-GVGFWKPGSGIIHQIILENYA--------------FPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRS-DIANLADKY---REVLLSPDG-GCKYDQVVEIDLNTLEPHVNGPFTPDLATPIGELGEKAK--------------------------KNGWPLEVKDG-----LIGSCTNSSYEDMARXTAII-KQALAKGMKSKS--NFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWD--RQNITKGEKN-----TIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAIKAPDGSELKLNAPF-GDELPSQ 1119          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000009029 (supercontig:LSalAtl2s:LSalAtl2s566:257091:257615:-1 gene:EMLSAG00000009029 transcript:EMLSAT00000009029 description:"augustus_masked-LSalAtl2s566-processed-gene-2.7")

HSP 1 Score: 76.6406 bits (187), Expect = 1.083e-16
Identity = 35/76 (46.05%), Postives = 48/76 (63.16%), Query Frame = 0

Query:   26 LRFGVKASGCSGFSYFIELGKQVNEDDQVFDSHGVKVVIDKQSLELVDGTEIDYVQDDLSALFKFNNPKVTASCGC 101
            LR GVK  GC+G SY ++   Q  + D+     GVK+ IDK++   + GTE+DYV  +LSA F FNNP +  +CGC
Sbjct:   54 LRIGVKQRGCNGMSYVLDYAIQKEKHDEEVIQDGVKIFIDKKAQLSILGTEMDYVDSNLSAEFVFNNPNIKGTCGC 129          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|88909585|sp|Q8NQ98.2|ACNA_CORGL (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1046.96 bits (2706), Expect = 0.000e+0
Identity = 518/920 (56.30%), Postives = 658/920 (71.52%), Query Frame = 0

Query:  101 CALPDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKD--------------------------------RDAKAGDVL--------TKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTR--YQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLE-----NGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
             A+P  +K   LP+SLK+L ENLLRTE+G +++   I+AI NWDA + P+ EI FTPARV+MQDFTGVP +VDLA MR+AV  LGGD   +NPL PAE+VIDHSV+V+ FG P AL KN E+E+ERN ERY+FLRWG  +F NF+VVPP TGIVHQVNIEYLAR VF    + E  AYPDT +GTDSHTTM NGLG+LGWGVGGIEAEAAMLGQ ++ML+P+VVGFKL G++P G TATD+VLT+T+MLR+ GVV KFVEFYG G+  +PLA+RATI NM+PE+G+TC +FPIDEET  YLRLTGR ++Q+ LV+ YA AQGM+  ED  EA Y+  LELD+ TVVPS+AGPKRPQDRI L++  E+  + +  Y  D                                  A +G+ L        +K     +  G  +T++ G V IA+ITSCTNTSNP V++ +GL+AR A  KGL  KPWVKT  APGS+VV  Y ++A+L  DL+ +GF + G+GCTTCIGNSGPLPE I  A+   DLT ++VLSGNRNFEGRI  D+KMN+LASP +V+AYA+AG +  D   + LGQDQ GNDVFLKDIWPS +EI++ +   I++ +++  Y++VFKGD++W  + +   D + W+ +STYI+  PYF GM  +P  +  I+ ARVLA LGDS+TTDHISPA S    +PA +YL E GV R D+NS GSRRGNH+VMMRGTFAN+RL+N+L     GG+TR   Q    +  IYDAS+ Y+  G+PL+V+ GKEYGTGSSRDWAAKGT LLG++AV+TES+ERIHRSNL+GMGV+PLQF  GES  S GL G E + I GL         + V +    E+G ++   + +VRIDTP E DYY+HGGIL YVLR++A S
Sbjct:   27 SAVPGMEK---LPYSLKVLGENLLRTEDGANITNEHIEAIANWDASSDPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVAALGGDPNDVNPLNPAEMVIDHSVIVEAFGRPDALAKNVEIEYERNEERYQFLRWGSESFSNFRVVPPGTGIVHQVNIEYLARVVF----DNEGLAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPVGVTATDVVLTITEMLRDHGVVQKFVEFYGSGVKAVPLANRATIGNMSPEFGSTCAMFPIDEETTKYLRLTGRPEEQVALVEAYAKAQGMWLDEDTVEAEYSEYLELDLSTVVPSIAGPKRPQDRILLSEAKEQFRKDLPTYTDDAVSVDTSIPATRMVNEGGGQPEGGVEADNYNASWAGSGESLATGAEGRPSKPVTVASPQGGEYTIDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAEKGLKSKPWVKTICAPGSQVVDGYYQRADLWKDLEAMGFYLSGFGCTTCIGNSGPLPEEISAAINEHDLTATAVLSGNRNFEGRISPDVKMNYLASPIMVIAYAIAGTMDFDFENEALGQDQDGNDVFLKDIWPSTEEIEDTIQQAISRELYEADYADVFKGDKQWQELDVPTGDTFEWDENSTYIRKAPYFDGMPVEPVAVTDIQGARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDEHGVERHDYNSLGSRRGNHEVMMRGTFANIRLQNQLV-DIAGGYTRDFTQEGAPQAFIYDASVNYKAAGIPLVVLGGKEYGTGSSRDWAAKGTNLLGIRAVITESFERIHRSNLIGMGVVPLQFPAGESHESLGLDGTETFDITGLTALNEGETPKTVKVTATKENG-DVVEFDAVVRIDTPGEADYYRHGGILQYVLRQMAAS 937          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81669260|sp|O53166.1|ACNA_MYCTU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1037.33 bits (2681), Expect = 0.000e+0
Identity = 527/914 (57.66%), Postives = 659/914 (72.10%), Query Frame = 0

Query:  110 ATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYA--------KDRDAKAGDVL-----------------------TKSAIEITQDGNT-------------FTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTR--YQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLE--NGSRHVDLVVNDESGKEIKRIEL--LVRIDTPREWDYYQHGGILHYVLRRLANS 973
            A LP+SLK+L ENLLR E+G +++K  I+AI NWD KA P+ EI +TPARVVMQDFTGVP IVDLA MR+A+  LGG+  K+NPLAPA+LVIDHSV+ D FG   A E+N E+E++RN ERY+FLRWGQ AFD+FKVVPP TGIVHQVNIEYLA  V T++      AYPDT VGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQ ++ML+P+VVGF+L G++  G TATD+VLTVT+MLR  GVVGKFVEFYGEG+A +PLA+RAT+ NM+PE+G+T  IFPIDEET+ YLR TGR  +Q+ LV+ YA AQGM+  + + E  ++  LEL++  VVPS+AGPKRPQDRIAL        E +  Y          D  +K  +V+                       T  +     DG               F L+ GAV IAAITSCTNTSNP V++ + LLARNAV KGLT KPWVKT++APGS+VV DY +++ L   L+KLGF +VGYGCTTCIGNSGPLPE I  AV + DL+V++VLSGNRNFEGRI+ D+KMN+LASPPLV+AYALAG +  D    PLGQD+ G +VFL+DIWPSQ+++ + + A INQ MF ++Y++VFKGD+RW  +P    + + W+ +STY++ PPYF GM+  P  +  I  ARVLA LGDS+TTDHISPAG+    +PA +YL E GV RKD+NS+GSRRGNH+VM+RGTFAN+RLRN+L     GG+TR   QP   +  IYDA+  Y    +PL+V  GKEYG+GSSRDWAAKGTLLLGV+AV+ ES+ERIHRSNL+GMGV+PLQF EG+S  S GL G E++ I G++  N  +    V    +  +   IE   +VRIDTP E DYY++GGIL YVLR +  S
Sbjct:   34 AKLPYSLKVLAENLLRNEDGSNITKDHIEAIANWDPKAEPSIEIQYTPARVVMQDFTGVPCIVDLATMREAIADLGGNPDKVNPLAPADLVIDHSVIADLFGRADAFERNVEIEYQRNGERYQFLRWGQGAFDDFKVVPPGTGIVHQVNIEYLASVVMTRDG----VAYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFRLTGEIQPGVTATDVVLTVTEMLRQHGVVGKFVEFYGEGVAEVPLANRATLGNMSPEFGSTAAIFPIDEETIKYLRFTGRTPEQVALVEAYAKAQGMWH-DPKHEPEFSEYLELNLSDVVPSIAGPKRPQDRIALAQAKSTFREQIYHYVGNGSPDSPHDPHSKLDEVVEETFPASDPGQLTFANDDVATDETVHSAAAHADGRVSNPVRVKSDELGEFVLDHGAVVIAAITSCTNTSNPEVMLGAALLARNAVEKGLTSKPWVKTTIAPGSQVVNDYYDRSGLWPYLEKLGFYLVGYGCTTCIGNSGPLPEEISKAVNDNDLSVTAVLSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFQTQPLGQDKDGKNVFLRDIWPSQQDVSDTIAAAINQEMFTRNYADVFKGDDRWRNLPTPSGNTFEWDPNSTYVRKPPYFEGMTAKPEPVGNISGARVLALLGDSVTTDHISPAGAIKPGTPAARYLDEHGVDRKDYNSFGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTQPGGPQAFIYDAAQNYAAQHIPLVVFGGKEYGSGSSRDWAAKGTLLLGVRAVIAESFERIHRSNLIGMGVIPLQFPEGKSASSLGLDGTEVFDITGIDVLNDGKTPKTVCVQATKGDGATIEFDAVVRIDTPGEADYYRNGGILQYVLRNILKS 942          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|38604821|sp|Q8CPC2.1|ACNA_STAES (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1011.52 bits (2614), Expect = 0.000e+0
Identity = 492/864 (56.94%), Postives = 640/864 (74.07%), Query Frame = 0

Query:  108 KIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEIT-QDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRH--VDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLR 968
            KI+ LP+S+++LLE++LR E+   ++   I+A+  +   A    E+ F P+RV++QDFTGVPA+VDLA++R A+  +GGD+ KINP  P +LVIDHSV VD +  P+ALE+N +LEFERN ERY+FL W   AFDN+  VPP TGIVHQVN+EYLA  V  ++ +GE TA+PDTLVGTDSHTTM+NG+GVLGWGVGGIEAEA MLGQ     +P+V+G +L   +P+G+TATDL L VT+ LR  GVVGKFVEF+G G+ HLPLADRATIANMAPEYGATCG FP+DEE+L Y++LTGR+++ IELVKEY     MF   ++ +  YT  ++LD+ TV  SL+GPKRPQD I L+DM ++  + V A A ++             EI   DG+  T++ G + IAAITSCTNTSNPYV++ +GL+A+ AV KGL V  +VKTSLAPGSKVVT YL  + L   LD LGF++VGYGCTTCIGNSGPL   I  AV + DL V+SVLSGNRNFEGRIH  +K N+LASP LVVAYALAG + +D+  +P+G+ + G DV+L+DIWPS +E+ + V   +   +F + Y NV+  +E W  I +T+   Y+++ +STYIQNP +F G+SK+P  I+P+K+ RV+   GDS+TTDHISPAG+   D+PAGKYL +  VA ++FNSYGSRRGNH+VM+RGTFAN+R++N+LAPGTEGG+T Y P    M IYDA+MKY+E+G  L+V+AG +YG GSSRDWAAKGT LLGVK V+ +SYERIHRSNLV MGVLPLQF++GES  + GL GKE  S++  E+   H  V++    E+G EI   + +VR D+  E DYY+HGGIL  VLR
Sbjct:   35 KISKLPYSIRVLLESVLRQEDDFVITDDHIKALSKF-GNAGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMFFDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINFNDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPGKIEPLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTGEIMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVDINEDVQPHDLVNVTAKKENG-EIINFKAIVRFDSLVELDYYRHGGILQMVLR 896          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81674773|sp|Q5HPJ0.1|ACNA_STAEQ (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1011.13 bits (2613), Expect = 0.000e+0
Identity = 492/864 (56.94%), Postives = 640/864 (74.07%), Query Frame = 0

Query:  108 KIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEIT-QDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRH--VDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLR 968
            KI+ LP+S+++LLE++LR E+   ++   I+A+  +   A    E+ F P+RV++QDFTGVPA+VDLA++R A+  +GGD+ KINP  P +LVIDHSV VD +  P+ALE+N +LEFERN ERY+FL W   AFDN+  VPP TGIVHQVN+EYLA  V  ++ +GE TA+PDTLVGTDSHTTM+NG+GVLGWGVGGIEAEA MLGQ     +P+V+G +L   +P+G+TATDL L VT+ LR  GVVGKFVEF+G G+ HLPLADRATIANMAPEYGATCG FP+DEE+L Y++LTGR+++ IELVKEY     MF   ++ +  YT  ++LD+ TV  SL+GPKRPQD I L+DM ++  + V A A ++             EI   DG+  T++ G + IAAITSCTNTSNPYV++ +GL+A+ AV KGL V  +VKTSLAPGSKVVT YL  + L   LD LGF++VGYGCTTCIGNSGPL   I  AV + DL V+SVLSGNRNFEGRIH  +K N+LASP LVVAYALAG + +D+  +P+G+ + G DV+L+DIWPS +E+ + V   +   +F + Y NV+  +E W  I +T+   Y+++ +STYIQNP +F G+SK+P  I+P+K+ RV+   GDS+TTDHISPAG+   D+PAGKYL +  VA ++FNSYGSRRGNH+VM+RGTFAN+R++N+LAPGTEGG+T Y P    M IYDA+MKY+E+G  L+V+AG +YG GSSRDWAAKGT LLGVK V+ +SYERIHRSNLV MGVLPLQF++GES  + GL GKE  S++  E+   H  V++    E+G EI   + +VR D+  E DYY+HGGIL  VLR
Sbjct:   35 KISKLPYSIRVLLESVLRQEDDFVITDDHIKALSKF-GNAGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMFFDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINFNDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPGKIEPLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTGEIMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVDINEDVQPHDFVNVTAKKENG-EIINFKAIVRFDSLVELDYYRHGGILQMVLR 896          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81600603|sp|Q5SMF6.1|ACNA_THET8 (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1009.98 bits (2610), Expect = 0.000e+0
Identity = 509/864 (58.91%), Postives = 643/864 (74.42%), Query Frame = 0

Query:  108 KIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPND-EIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRT-EDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLEN--GSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVL 967
            +++ LPFS++++LE+LLR E+G  V++ DI+A+  W  +  P +  +    ARV++QDFTGVPA+VDLAAMRDA+   GGD ++INP+ PA+LVIDHSV VD FGT  A   N E E+ERN ERY  L+W Q+A +NF+VVPP TGIVHQVNIEYL + V T + +G   A+PD+LVGTDSHTTMVNGLGVLGWGVGGIEAEA MLGQ   ML P+VVGFKL G++PEGATATDLVLTVT+MLR  GVVGKFVEFYG G+A L   DRATIANMAPEYGAT G FP+DEETL YLR TGR ++ +ELV+ Y  A G+FRT E + + +Y+  LELD+  V PSLAGPKRPQDR+ L ++ +    ++    K+R     +   +  + + +    F L  G+V IAAITSCTNTSNP V++ +GLLA+ AV  GL  KPWVKTSLAPGSKVVTDYLE + LM  L+ LGF +VGYGCTTCIGNSGPLPE I  AV  G+L V++VLSGNRNFEGRI+  +K N+LASP LVVAYALAG + +D   +PLG D  G  ++LKDIWPS +EI+E +   ++  +FKK YS VF+GDERW A+P    + Y W+ +STYIQNPP+F  + +    ++ I+ ARVL  LGDS+TTDHISPAG+    SPAG+YL  +GV  +DFNSYGSRRGNH+VMMRGTFAN+R++N +  G EGG+ +  PE     +Y+ +M+Y+  G PL+VIAGKEYGTGSSRDWAAKGT LLG++AV+ ES+ERIHRSNLVGMGVLPL+F  GE++ + GLTG E+Y I GLE+    + VD+V   E G E+ R + + R+DTP E DYY++GGIL  VL
Sbjct:   33 EVSRLPFSIRVMLESLLRNEDGYQVTREDIEALARW--RPDPGEINVPLKLARVILQDFTGVPAVVDLAAMRDAIKAKGGDPKRINPVVPADLVIDHSVQVDAFGTAYAFFYNVEKEYERNRERYLLLKWAQNALENFRVVPPGTGIVHQVNIEYLTKVVMTGKRDGLTLAFPDSLVGTDSHTTMVNGLGVLGWGVGGIEAEAVMLGQPYYMLAPRVVGFKLYGELPEGATATDLVLTVTEMLRKHGVVGKFVEFYGPGVAKLSTPDRATIANMAPEYGATMGFFPVDEETLNYLRQTGRPEELVELVEAYTKAVGLFRTPEAEEKVQYSEYLELDLSAVEPSLAGPKRPQDRVPLKEVKKSFLAHLTKPVKERGFGLSEDQLQRKVLVKRRDEEFELTHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLDRKPWVKTSLAPGSKVVTDYLEMSGLMPFLEALGFHLVGYGCTTCIGNSGPLPEDIAKAVEEGNLVVAAVLSGNRNFEGRINPHVKANYLASPMLVVAYALAGRMDIDFTTEPLGFDPNGKPIYLKDIWPSMEEIREAIRKTLDPELFKKEYSKVFEGDERWQALPAPTGELYQWDPESTYIQNPPFFEDLGE--RKVEDIRGARVLLVLGDSVTTDHISPAGAIPVKSPAGQYLISKGVKPEDFNSYGSRRGNHEVMMRGTFANIRIKNLMLDGIEGGYAKKLPEGDVDFVYNVAMRYKAEGTPLLVIAGKEYGTGSSRDWAAKGTYLLGIRAVLAESFERIHRSNLVGMGVLPLEFLPGENRETLGLTGYEVYDILGLEDLKPRKLVDIVARREDGSEV-RFQAIARLDTPVEVDYYKNGGILQTVL 891          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|2506131|sp|P09339.4|ACNA_BACSU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1003.05 bits (2592), Expect = 0.000e+0
Identity = 484/867 (55.82%), Postives = 630/867 (72.66%), Query Frame = 0

Query:  108 KIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQ-DGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLV----VNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRR 969
            K++ LP+S+K+LLE++LR  +G  + K  ++ +  W      + ++ F P+RV++QDFTGVPA+VDLA++R A+  +GGD  KINP  P +LVIDHSV VD  GT  AL  N +LEFERNAERY+FL W + AF+N++ VPP TGIVHQVN+E+LA  V   EE+GE+  YPDTLVGTDSHTTM+NG+GVLGWGVGGIEAEA MLGQ     VP+V+G KL GK+P G TATDL L VTQ+LR  GVVGKFVEF+G G+A LPLADRATIANMAPEYGATCG FP+DEE L YLRLTGR+ + I++V+ Y  + G+F T D  + ++T  +E+D+  +  +L+GPKRPQD I L+ M E   + + + A ++            I+    +G    ++ GA+ IAAITSCTNTSNPYV++ +GL+A+ AV  GL V  +VKTSLAPGSKVVT YL  + L+  + +LGF++VGYGCTTCIGNSGPL   I  AV   DL ++SVLSGNRNFEGRIH  +K N+LASPPLVVAYALAG + +++  DP+G  + G +V+  DIWPS  EI  +V   +   +F+K Y  VF  ++RW  I  T+   Y W++DSTYIQNPP+F  MS +P  ++P+K  RV+   GDS+TTDHISPAG+   D+PAGKYL E+GV+ +DFNSYGSRRGNH+VMMRGTFAN+R++N++APGTEGG+T Y P     SIYDA MKY+E+   L+V+AGK+YG GSSRDWAAKGT LLG++ V+ ES+ERIHRSNLV MGVLPLQFK+GE+  + GLTGKE+  ++ ++   R  DLV    +N++    +   E +VR D+  E DYY+HGGIL  VLR 
Sbjct:   41 KVSKLPYSIKVLLESVLRQVDGFVIKKEHVENLAKWGTAELKDIDVPFKPSRVILQDFTGVPAVVDLASLRKAMAAVGGDPDKINPEIPVDLVIDHSVQVDKAGTEDALAVNMDLEFERNAERYKFLSWAKKAFNNYQAVPPATGIVHQVNLEFLASVVHAIEEDGELVTYPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPVPEVIGAKLVGKLPNGTTATDLALKVTQVLREKGVVGKFVEFFGPGIAELPLADRATIANMAPEYGATCGFFPVDEEALNYLRLTGRDPEHIDVVEAYCRSNGLFYTPDAEDPQFTDVVEIDLSQIEANLSGPKRPQDLIPLSAMQETFKKQLVSPAGNQGFGLNAEEENKEIKFKLLNGEETVMKTGAIAIAAITSCTNTSNPYVLIGAGLVAKKAVELGLKVPNYVKTSLAPGSKVVTGYLVNSGLLPYMKELGFNLVGYGCTTCIGNSGPLSPEIEEAVAKNDLLITSVLSGNRNFEGRIHPLVKGNYLASPPLVVAYALAGTVNINLKTDPIGVGKDGQNVYFNDIWPSMDEINALVKQTVTPELFRKEYETVFDDNKRWNEIETTDEALYKWDNDSTYIQNPPFFEEMSVEPGKVEPLKGLRVVGKFGDSVTTDHISPAGAIGKDTPAGKYLQEKGVSPRDFNSYGSRRGNHEVMMRGTFANIRIKNQIAPGTEGGFTTYWPTGEVTSIYDACMKYKEDKTGLVVLAGKDYGMGSSRDWAAKGTNLLGIRTVIAESFERIHRSNLVFMGVLPLQFKQGENADTLGLTGKEVIEVD-VDETVRPRDLVTVRAINEDG--NVTTFEAVVRFDSEVEIDYYRHGGILQMVLRE 904          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|122426093|sp|Q1RKD5.1|ACNA_RICBR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 991.875 bits (2563), Expect = 0.000e+0
Identity = 490/868 (56.45%), Postives = 631/868 (72.70%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSR---HVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            +  LP+SL++L EN+LRT      SK ++     W      + EI F PARV+MQDFTGVPAIVDLAAMRDA+ K+GGD  KINPL P +LVIDHSV VD + +  + +KN  +E  RN ERY+FL+WGQ AF+NFKVVPP TGI HQVN+EYLA+ V+    NG   AYPD+LVGTDSHTTMVNGL VLGWGVGGIEAEAAMLGQ +TM++P+V+G KL GK+   ATATDLVL +T+MLR   VVGKFVEFYGEGL  + +ADRATI+NMAPEYGATCG FPID+ET+ YL LTGR+ +QI+LV+EYA AQ ++   D   A YT  LELD+  V  SLAGP+RPQDR+ L D+S    + +  ++ +  +    + TK A+        + + +G V IAAITSCTNTSNP V++ + LLA+ A+ +GL VKPWVKTSLAPGSKVVT+YL+ + L   LD+LGF++VGYGCTTCIGNSGPL   I   +    L V+SVLSGNRNFEGRI+   K ++LASP LVVAYAL+G++ +D+   PLG++ +G DV+LKDIWPS++EI +V+  +IN +MF + YS++F G + W ++ +T S  Y W+  STYI NPPYF  +    S IK IK+AR+LA  GDSITTDHISPAGS +  SPA KYLT+  ++  DFNSYGSRRGNH+VMMRGTFAN+R++NE+  G EGG+T  Q ++ + +IYDA+M Y+ NGV  ++ AGKEYG+GSSRDWAAKG  LLGVKAV+ ES+ERIHRSNLVGMGVLPL F    ++    L G E   I GL    +    V  ++  ++G E++ I+L+++I T  E +Y +HG I+H+V+  L N+
Sbjct:   36 LKKLPYSLRVLFENVLRTG-----SKQNLMVFKEWLKNKKSDAEIDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYASGSSFDKNVAMEMRRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVW--HSNG--VAYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGTATATDLVLKITEMLRKKKVVGKFVEFYGEGLKAMTIADRATISNMAPEYGATCGFFPIDQETIKYLELTGRDKEQIKLVEEYAKAQDLWCNFDDA-AEYTDILELDLSEVTSSLAGPRRPQDRVNLGDVSSGFKKELSTFSSNNIS----IDTKHAVA----NQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKAIEQGLKVKPWVKTSLAPGSKVVTEYLKSSGLNQYLDQLGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRINPLTKASYLASPILVVAYALSGSLNIDLTNHPLGKNDKGRDVYLKDIWPSKEEIDKVIANSINSSMFVEKYSDIFSGTKEWQSLEVTSSSNYAWDKSSTYINNPPYFENIGSKNS-IKDIKSARILAIFGDSITTDHISPAGSISKTSPAAKYLTDHQISPIDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLKNMQQTIYDAAMDYKANGVSAVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGVLPLIFTNNMTRFDLKLDGSESIDIIGLNEHIKPYNSVKCIIKKQNG-EMQTIDLILQIFTDNEINYIKHGSIMHFVVENLKNN 883          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|60391209|sp|Q6GH55.1|ACNA_STAAR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 989.178 bits (2556), Expect = 0.000e+0
Identity = 495/868 (57.03%), Postives = 635/868 (73.16%), Query Frame = 0

Query:  108 KIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAI----EIT-QDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRH--VDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLR 968
            K++ LP+S+++LLE+LLR E+   ++   I+A+  +  K     E+ F P+RV++QDFTGVPA+VDLA++R A+  +GGD+ KINP  P +LVIDHSV VD +  P+ALE+N +LEFERN ERY+FL W   AFDN+  VPP TGIVHQVN+EYLA  V  ++ +GE TA+PDTLVGTDSHTTM+NG+GVLGWGVGGIEAEA MLGQ     +P+V+G +L   +P+GATATDL L VTQ LR  GVVGKFVEF+G G+ HLPLADRATIANMAPEYGATCG FP+D+E+L Y++LTGR D+ I LVKEY     MF   ++ +  YT  +ELD+ TV  SL+GPKRPQD I L+DM       V A A ++    G  L KS      EI  +DG+  T++ G + IAAITSCTNTSNPYV++ +GL+A+ AV KGL V  +VKTSLAPGSKVVT YL  A L   LD LGF++VGYGCTTCIGNSGPL   I  A+ + DL V+SVLSGNRNFEGRIH  +K N+LASP LVVAYALAG++ +D+  +P+G+   G DV+LKDIWPS KE+ + V + +   +F + Y+NV+  +E W  I +T+   Y+++ +STYIQNP +F G+SK+P  I P+   RV+   GDS+TTDHISPAG+   D+PAGKYL +  V  ++FNSYGSRRGNH+VM+RGTFAN+R++N+LAPGTEGG+T Y P +  M I+DA+MKY+E+G  L+V+AG +YG GSSRDWAAKGT LLGVK V+ +SYERIHRSNLV MGVLPL+FK+GES  S GL G E  S+   EN   H  V +    + G ++   + +VR D+  E DYY+HGGIL  VLR
Sbjct:   35 KVSKLPYSIRVLLESLLRQEDDFVITDDHIKALSQF-GKDGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSDEHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKSSFENSVTAPAGNQ----GHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRGAGLQPYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGSVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYNNNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYVKVTAKKQDG-DLVEFDAMVRFDSLVEMDYYRHGGILQMVLR 896          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|54036686|sp|P63434.1|ACNA_STAAW (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|54036700|sp|P99148.1|ACNA_STAAN RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|54040671|sp|P63433.1|ACNA_STAAM RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|60391208|sp|Q6G9K9.1|ACNA_STAAS RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|81694556|sp|Q5HG69.1|ACNA_STAAC RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 988.793 bits (2555), Expect = 0.000e+0
Identity = 495/868 (57.03%), Postives = 634/868 (73.04%), Query Frame = 0

Query:  108 KIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAI----EIT-QDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRH--VDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLR 968
            K++ LP+S+++LLE+LLR E+   ++   I+A+  +  K     E+ F P+RV++QDFTGVPA+VDLA++R A+  +GGD+ KINP  P +LVIDHSV VD +  P+ALE+N +LEFERN ERY+FL W   AFDN+  VPP TGIVHQVN+EYLA  V  ++ +GE TA+PDTLVGTDSHTTM+NG+GVLGWGVGGIEAEA MLGQ     +P+V+G +L   +P+GATATDL L VTQ LR  GVVGKFVEF+G G+ HLPLADRATIANMAPEYGATCG FP+D+E+L Y++LTGR D+ I LVKEY     MF   ++ +  YT  +ELD+ TV  SL+GPKRPQD I L+DM       V A A ++    G  L KS      EI  +DG+  T++ G + IAAITSCTNTSNPYV++ +GL+A+ AV KGL V  +VKTSLAPGSKVVT YL  A L   LD LGF++VGYGCTTCIGNSGPL   I  A+ + DL V+SVLSGNRNFEGRIH  +K N+LASP LVVAYALAG + +D+  +P+G+   G DV+LKDIWPS KE+ + V + +   +F + Y+NV+  +E W  I +T+   Y+++ +STYIQNP +F G+SK+P  I P+   RV+   GDS+TTDHISPAG+   D+PAGKYL +  V  ++FNSYGSRRGNH+VM+RGTFAN+R++N+LAPGTEGG+T Y P +  M I+DA+MKY+E+G  L+V+AG +YG GSSRDWAAKGT LLGVK V+ +SYERIHRSNLV MGVLPL+FK+GES  S GL G E  S+   EN   H  V +    + G ++   + +VR D+  E DYY+HGGIL  VLR
Sbjct:   35 KVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQF-GKDGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSDEHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKSSFENSVTAPAGNQ----GHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYNNNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYVKVTAKKQDG-DLVEFDAMVRFDSLVEMDYYRHGGILQMVLR 896          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|75535948|sp|Q4UK20.1|ACNA_RICFE (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 971.074 bits (2509), Expect = 0.000e+0
Identity = 484/865 (55.95%), Postives = 627/865 (72.49%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSR---HVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRL 970
            +  LP+SL++L EN+LR+ NG   SK ++     W      + EI F PARV+MQDFTGVPAIVDLAAMRDA+ K+GGD  KINPL P +LVIDHSV VD +    + +KN ++E +RN ERY+FL+WGQ AF+NFKVVPP TGI HQVN+EYLA+ V+ K+      AYPD+LVGTDSHTTMVNGL VLGWGVGGIEAEAAMLGQ +TM++P+V+G KL GK+   ATATDLVLTVT+MLR   VVGKFVEF+GEGL +L +ADRATI+NM+PEYGATCG FPID+ET+ YL LTGRE  QI+LV++YA  Q ++  + + EA YT  LELD+  V  SLAGP+RPQDR+ L D++      +  +  +      D+  K A+        + + +G V IAAITSCTNTSNP V++ + LLA+ A+  GL VKPWVKTSLAPGSKVVT+YL+ + L   LD+LGF++VGYGCTTCIGNSGPL   I   +    L V+SVLSGNRNFEGRI+   K ++L SP LVVAYAL+G + +D++  P+G++     ++LKDIWPS++EI EV+  +IN +MF + YS++F G + W  + IT S  YNWN +STYI NPPYF  +    ++IK IK+A++LA  GDSITTDHISPAGS +  SPA KYLT+  +   DFNSYGSRRGNH+VMMRGTFAN+R++NE+  G EGG+T  Q   ++ +IYDA+M Y+ N VP+++ AGKEYG+GSSRDWAAKG  LLGVKAV+ ES+ERIHRSNLVGMG+LPL F    +++   L G E   I GL    +    V  ++  ++G E + I+L+++I T  E +Y +HG I+H+V+  L
Sbjct:   36 LKKLPYSLRVLFENVLRS-NG---SKQNLLVFKEWLKTKESDAEIDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYAAKDSFDKNVQMEMKRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVWHKDG----LAYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGIATATDLVLTVTEMLRKKKVVGKFVEFFGEGLKNLTIADRATISNMSPEYGATCGFFPIDQETIKYLELTGREKTQIKLVEKYANEQNLWY-DFEHEAEYTEILELDLSMVHSSLAGPRRPQDRVDLNDVANNFKHELPNFGIENK----DIDKKYAVA----NQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKALEHGLKVKPWVKTSLAPGSKVVTEYLKLSGLDKYLDELGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRINPLTKASYLGSPILVVAYALSGTLNIDLNNQPIGKN-----IYLKDIWPSKEEIDEVIANSINSSMFIEKYSDIFSGTKEWKDLQITTSSTYNWNKNSTYINNPPYFEDIGSK-NNIKDIKSAKILAIFGDSITTDHISPAGSISKTSPAAKYLTDNHIEPLDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLSSTQQTIYDAAMDYKANDVPVVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGILPLTFTGNNTRLDLKLDGSETIDIIGLSEQIKPYNPVKCMIKKQTG-ETRTIDLILQIFTDNEINYIKHGSIMHFVVENL 876          

BLAST of aconitate hydratase vs. nr
Match: gi|1085192357|gb|OGT79362.1| (aconitate hydratase 1 [Gammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_61_13])

HSP 1 Score: 1231.47 bits (3185), Expect = 0.000e+0
Identity = 594/863 (68.83%), Postives = 709/863 (82.16%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            +ATLP+SLKILLENLLR E+G+++   DI+A+  WD KA P+ EIAFTPARV+MQDFTGVPA+VDLAAMRDA+ +LGGD  +INPL+PAELVIDHSV VD FG+  A E+NAE+EFERN ERYEFLRWGQ AF NFKVVPP TGIVHQVN+E+LART+F +E+NG + A+PDT+VGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ITML+PQV+GF+L G++PEGATATDLVLTVTQMLR  GVVGKFVEF+G GLA LPLADRATIANMAPEYGATCGIFPIDEETL YLRL+GRED QI LV+ YA  QGM+R    P ARYT T+ LDMG++ P LAGPKRPQDR+ L D+ + + + +      R   AGD   +SA  +   G +F ++DGAV IAAITSCTNTSNP V+V +GLLA+ A  +GLT KPWVKTSLAPGS VV DYL+KA L   LD++GF++VGYGCTTCIGNSGPLPEAI   +R  DL VS+VLSGNRNFEGRIHA++KMN+LASPPLVVAYA+AGN+ VD+  D LG D  G  V+L+DIWP+QKEI + + A IN  MF+KSY NV++GD RW+ + +  ++RY W  DSTY+QNPPYFVGMSK P  IK IK ARVLA LGDSITTDHISPAG+   DSPAG+YL E GVA  DFNSYGSRRGNH+VMMRGTFAN+RLRN L  G EGG T++ P   ++SIYDASM+YQ++ VPL+++AGKEYGTGSSRDWAAKGTLLLGV+AV+ ES+ERIHRSNLVGMGVLPL +++GE+  S GL G E+Y+IEGL+ G+  V +V  D+SGK  KR++  VR+DTP+EW+YY +GGILHYVLR+LA
Sbjct:   32 VATLPYSLKILLENLLRFEDGINIEAGDIEALAKWDPKAEPDKEIAFTPARVLMQDFTGVPAVVDLAAMRDAMARLGGDPARINPLSPAELVIDHSVQVDVFGSANAAERNAEIEFERNQERYEFLRWGQQAFSNFKVVPPDTGIVHQVNLEFLARTIFAQEKNGVLEAFPDTVVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPITMLIPQVIGFELTGRLPEGATATDLVLTVTQMLRKKGVVGKFVEFFGSGLATLPLADRATIANMAPEYGATCGIFPIDEETLRYLRLSGREDQQIALVEAYAKEQGMWRDPAAPVARYTDTMTLDMGSIEPCLAGPKRPQDRVTLRDVRKVSQDRMGEMQAARKGGAGD---RSA-SVNHGGKSFEVKDGAVVIAAITSCTNTSNPSVMVGAGLLAKKARERGLTAKPWVKTSLAPGSLVVRDYLDKAGLTPYLDQMGFNIVGYGCTTCIGNSGPLPEAISEGIRKEDLVVSAVLSGNRNFEGRIHAEVKMNYLASPPLVVAYAIAGNMNVDLQNDSLGNDAAGKPVYLRDIWPTQKEINDAMAAAINSGMFRKSYGNVYQGDARWSGLKVPSTNRYEWRGDSTYVQNPPYFVGMSKAPPGIKQIKGARVLAKLGDSITTDHISPAGNIKKDSPAGRYLVEHGVAPADFNSYGSRRGNHEVMMRGTFANIRLRNLLLTGVEGGMTKHMPSGEQLSIYDASMRYQKDNVPLVILAGKEYGTGSSRDWAAKGTLLLGVRAVLAESFERIHRSNLVGMGVLPLTYRDGENAESLGLAGTEVYTIEGLKEGATEVTVVATDDSGKS-KRLQARVRVDTPKEWEYYTNGGILHYVLRQLA 889          

BLAST of aconitate hydratase vs. nr
Match: gi|739473592|ref|WP_037333493.1| (aconitate hydratase AcnA [Salinisphaera hydrothermalis] >gi|666936519|gb|KEZ78951.1| aconitate hydratase [Salinisphaera hydrothermalis C41B8])

HSP 1 Score: 1230.7 bits (3183), Expect = 0.000e+0
Identity = 602/880 (68.41%), Postives = 708/880 (80.45%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIE---------------ITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            +A LP++ KILLENLLR E+G +V+  DI+A+ NWDAKA P+ EIAFTPARVV+QDFTGVPA+VDLAAMRDA+  LGG   KINP +PAELVIDHSVMVD +G+ +A + NA+LEF RN ERY FLRWGQ AFDNFKVVPP TGIVHQVN+EYLAR VF  E+ G   AYPDTLVGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQ ITML+PQV+GFKL GK+ EG TATDLVLTVT+MLR  GVVGKFVEFYG+GLA L LADRATIANMAPEYGATCGIFP+DEET+ Y+ LTGR+ +Q+EL +EYA AQG++R   QP+A YT +LELD+ TV PSLAGPKRPQDR+ L+DM       V+ + + R+ KAG  + ++  E               +      F L DG+V IAAITSCTNTSNP V++ +GLLARNA+ KG+TVKPWVKTSLAPGSKVVT+YLEKA L  DLD+LGF++VGYGCTTCIGNSGPLP+ IG AVRN +L V+SVLSGNRNFEGR+H D++MNFLASPPLVVAYALAG I  D+  DPLGQD  GNDVFL D+WPSQKEI + +  ++N  MFK+SY +VF GD RW  + + E + Y W+ DSTY+QNPPYF GM+ D  DI  IK AR LA LGDSITTDHISPAG+   DSPAG+YL EQGV   DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGGWTRYQPE  EM IYDA+MKY++ G PL+++AGKEYGTGSSRDWAAKGTLLLGVKAV+ ES+ERIHRSNLVGMGVLPLQF EG+S  S GL G E + ++GL  G++ V +    ESG+ ++  +  VRIDTP+EWDYYQ+GGILHYVLR+LA +
Sbjct:   33 VAKLPYAQKILLENLLRHEDGANVTADDIKALANWDAKAEPDTEIAFTPARVVLQDFTGVPAVVDLAAMRDAMKNLGGSPAKINPQSPAELVIDHSVMVDEYGSDKAFDLNAKLEFNRNKERYAFLRWGQGAFDNFKVVPPDTGIVHQVNLEYLARVVFGNEDTG--LAYPDTLVGTDSHTTMINGVGVLGWGVGGIEAEAAMLGQPITMLIPQVIGFKLTGKLAEGTTATDLVLTVTEMLRAKGVVGKFVEFYGDGLADLALADRATIANMAPEYGATCGIFPVDEETIRYMELTGRDAEQLELTREYAKAQGLWREAGQPDANYTDSLELDLSTVQPSLAGPKRPQDRVLLSDMQATYKREVKPFIEAREEKAGKSMAEAKEESEGGIPSKSVGGTARVNYKDAEFDLHDGSVVIAAITSCTNTSNPAVMIGAGLLARNAINKGMTVKPWVKTSLAPGSKVVTEYLEKAGLDDDLDQLGFNLVGYGCTTCIGNSGPLPDPIGEAVRNHNLNVASVLSGNRNFEGRVHGDVRMNFLASPPLVVAYALAGTIDTDLTNDPLGQDADGNDVFLADLWPSQKEIYDTIGTSLNSEMFKQSYGDVFAGDSRWQNLDVPEGEIYAWDEDSTYVQNPPYFEGMTVDVDDIPTIKGARCLAKLGDSITTDHISPAGAITQDSPAGQYLQEQGVKPADFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGWTRYQPEGREMFIYDAAMKYKDEGTPLVILAGKEYGTGSSRDWAAKGTLLLGVKAVIAESFERIHRSNLVGMGVLPLQFNEGDSHESLGLDGSETFDLDGLAAGAKSVHITATKESGETVE-FDARVRIDTPKEWDYYQNGGILHYVLRQLAKA 909          

BLAST of aconitate hydratase vs. nr
Match: gi|1180041561|ref|WP_083562433.1| (aconitate hydratase AcnA [Oceanococcus atlanticus] >gi|1178157080|gb|ORE86237.1| aconitate hydratase 1 [Oceanococcus atlanticus])

HSP 1 Score: 1229.54 bits (3180), Expect = 0.000e+0
Identity = 583/880 (66.25%), Postives = 698/880 (79.32%), Query Frame = 0

Query:  102 ALPDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKA----------GDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            A+  Q ++  LP+SLK+LLENL+R E+GV+++K DI A+INWD KA P+ EIAFTPARV++QDFTGVP +VDLAAMRDAV KLGGD  KINPL P ELVIDHSVMVD  G+  AL+ NA++EF+RN ERY FLRWGQ+AFDNFKVVPP TGIVHQVNIEYLAR VF KE      AYPDT+VGTDSHTTMVNG+GVLGWGVGGIEAEAAMLGQ +TML+PQVVG++L GK+ EG TATDLVLT+ + LR LGVVGKFVEF+GEGLA LPLADRATIANMAPEYGATCGIFP+DE T+ YL+LTGR++D++ LV+ YA A GM+R +D P+A Y+  LELD+ TV PSLAGPKRPQDRI L+DM      +++    +R  K+          G V+     ++  DG +F L DGAV IAAITSCTNTSNP V++ +GL+A+ A A+GL VKPWVKTSLAPGS+VVTDYL KA LM DL+ LGF VVGYGCTTCIGNSGPL  AI  A+++ DL  +SVLSGNRNFEGR+H+D++MNFLASPPLVVAYALAG + +D+  +PLGQDQ GNDV+LKDIWPS  EIQE + A++N  MFK  Y++V+ GD RW  + I     Y W++ STY+QNPPYF GMS D  DI  IK AR LA LGDSITTDHISPAG    D  AG+YL  +GV + DFNS GSRRGNH+VMMRGTFANVRLRN++APGTEGGWTR+ P+  +MSIY+A+MKY+    PL+V+ GKEYGTGSSRDWAAKGT+LLGVKAV+ ES+ERIHRSNLVGMGVLPLQF +G+S  S GLTG E Y IEGL+ G++ V +    + GK +   +  VRIDTP+EWDYY+HGGIL YV+R+LA
Sbjct:   25 AVASQAQLDRLPYSLKVLLENLVRHEDGVNITKDDINALINWDPKAEPSTEIAFTPARVILQDFTGVPCVVDLAAMRDAVAKLGGDATKINPLVPVELVIDHSVMVDKSGSNDALDLNAKIEFDRNKERYAFLRWGQTAFDNFKVVPPDTGIVHQVNIEYLARVVFGKETG---VAYPDTVVGTDSHTTMVNGIGVLGWGVGGIEAEAAMLGQPVTMLIPQVVGYRLTGKLSEGVTATDLVLTIVERLRELGVVGKFVEFFGEGLADLPLADRATIANMAPEYGATCGIFPVDEATINYLKLTGRDEDELALVETYAKANGMWRHDDDPDAEYSHVLELDLSTVQPSLAGPKRPQDRILLSDMKSSYQSHLKPMVAERSEKSQAEARLESEGGPVVKSGVAQLAIDGESFELSDGAVVIAAITSCTNTSNPVVLLGAGLVAQKAAARGLKVKPWVKTSLAPGSQVVTDYLNKAGLMDDLEALGFHVVGYGCTTCIGNSGPLKAAISQAIQDNDLVATSVLSGNRNFEGRVHSDVRMNFLASPPLVVAYALAGTVDIDLVNEPLGQDQDGNDVYLKDIWPSNAEIQETLAASLNSDMFKTRYADVYAGDARWQGLEIPTGKIYEWDNTSTYVQNPPYFEGMSLDTDDIGEIKGARCLAKLGDSITTDHISPAGVIKPDQDAGEYLMSKGVQQADFNSLGSRRGNHEVMMRGTFANVRLRNQIAPGTEGGWTRHLPDGEQMSIYEAAMKYKAENTPLVVLGGKEYGTGSSRDWAAKGTILLGVKAVICESFERIHRSNLVGMGVLPLQFADGDSHESLGLTGLETYDIEGLQRGAKSVSVTATTDDGK-VTTFQAKVRIDTPKEWDYYEHGGILQYVIRQLA 900          

BLAST of aconitate hydratase vs. nr
Match: gi|1085188777|gb|OGT76241.1| (aconitate hydratase 1 [Gammaproteobacteria bacterium RIFCSPLOWO2_12_47_11])

HSP 1 Score: 1220.3 bits (3156), Expect = 0.000e+0
Identity = 603/867 (69.55%), Postives = 699/867 (80.62%), Query Frame = 0

Query:  106 QDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNT-FTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLA 971
            Q  I+ LP+SLKILLENLLR E+G +V KADI A+  WD KA P+ EIAFTPARV+MQDFTGVPA+VDLAAMRDA+ +LGGD +KINPL+PAELVIDHSVMVDYF  P A EKNAE+EFERN ERY FLRWGQ AF NFKVVPP TGI HQVN+EYLARTVFT+  NG   AYPDT+VGTDSHTTM+NGLGVLGWGVGGIEAEAAMLGQ ITML+PQVVGFKL GK+PEGATATDLVLTVT MLR  GVVGKFVE++G GL +LPLADRAT+ANMAPEYGATCGIFPID ETL YLRL+GR ++QI LV+ YA AQGM+R + + EA Y+  LELDMG VVPSLAGPKRPQDR+ALTDM +     +    + R        TK       DG T + L DGAV IAAITSCTNTSNP V++ +GLLA+ A   GLT KPWVKTSLAPGS VV+DYL+KA L+  L  LGFD VGYGCTTCIGNSGPLPEA+   +  GDL V+SVLSGNRNFEGR+HA+IKMNFLASPPLVVAY++AG +  D++KDPLG  + G  V+LKDIWPS +EI + + + I+  MF+ SY++VFKGD RW +I +  SDRY+W   STYIQNPPYFVGMSK       I+ ARVLA LGDSITTDHISPAG+   D PAGKYL E GV  KDFNSYG+RRGNH+VMMRGTFAN+RLRN+LAPGTEGGWT +QP   +M IYDASMKYQ  G PLI++AGKEYGTGSSRDWAAKGT+LLG +AV+ ES+ERIHRSNLVGMGVLPL FK+GE+  S GLTGKE+Y I+GL+NG++ V ++   +  KEI R +  VR+DTP+EW+YY +GGILHYVLR+LA
Sbjct:   29 QHDISRLPYSLKILLENLLRHEDGENVRKADIAALAEWDPKAEPDQEIAFTPARVLMQDFTGVPAVVDLAAMRDAMSQLGGDPKKINPLSPAELVIDHSVMVDYFAQPDAAEKNAEIEFERNHERYVFLRWGQEAFSNFKVVPPDTGICHQVNLEYLARTVFTRTVNGVEQAYPDTVVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPITMLIPQVVGFKLIGKLPEGATATDLVLTVTNMLRKKGVVGKFVEYFGPGLDNLPLADRATLANMAPEYGATCGIFPIDVETLNYLRLSGRSEEQITLVEAYAKAQGMWR-DSKLEANYSDVLELDMGRVVPSLAGPKRPQDRVALTDMQQTFKTSLPPMVEKR--------TKPGKATVTDGKTSYELTDGAVVIAAITSCTNTSNPSVMIGAGLLAKKAHEMGLTRKPWVKTSLAPGSLVVSDYLKKAGLVPHLAALGFDTVGYGCTTCIGNSGPLPEAVSKGIAEGDLVVASVLSGNRNFEGRVHAEIKMNFLASPPLVVAYSIAGTVNTDLNKDPLGTGKDGKPVYLKDIWPSSQEISKAIASTIDSKMFQGSYAHVFKGDTRWGSIKVPASDRYHWEDSSTYIQNPPYFVGMSKQAQGFDQIQGARVLALLGDSITTDHISPAGAIKKDGPAGKYLIEHGVLHKDFNSYGARRGNHEVMMRGTFANIRLRNQLAPGTEGGWTIHQPSGEQMFIYDASMKYQAEGTPLIILAGKEYGTGSSRDWAAKGTMLLGCRAVIAESFERIHRSNLVGMGVLPLTFKQGENAESLGLTGKEVYDIDGLKNGAKEVTVIAKGD--KEI-RFKATVRVDTPKEWEYYNNGGILHYVLRQLA 883          

BLAST of aconitate hydratase vs. nr
Match: gi|1268755610|ref|WP_099019639.1| (aconitate hydratase AcnA [Marinicella litoralis])

HSP 1 Score: 1218.76 bits (3152), Expect = 0.000e+0
Identity = 576/865 (66.59%), Postives = 689/865 (79.65%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENG-SRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLAN 972
            I+ LPFSLKILLENLLR ENG+DV+ +DI+A+ NWD  A P  EIAFTP+RVV+QDFTGVPA+VDLAAMRDA+  LGGDVQKINPL+PAELVIDHS+ VD +GT  + E N ++EF+RN ERY FL+WGQ+AFD FKVVPP  GIVHQVN+EYLAR +F  E+NGE+ AYPDT+VGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQAI+ML+PQVVGFKL GKM EG TATDLVLTV  MLR  GVVGKFVEF+G GL HLPLADRATIANMAPEYGATCGIFPID E+L YL L+GR ++Q+ LV+ YA AQGMF  E+  +A YTA LELDM TVVPS++GPKRPQDRI LT   E    + + +   R  K+        + ++ D   +   DG + +AAITSCTNTSNP V++ +GLLARNA AKGL VKPWVKTSLAPGSKVV+ YL  A +M DL+ LGF+VVG+GCTTCIGNSGPL  A+  A+ + DL  +SVLSGNRNFEGRIHADI+MNFLASPPLVVAYA+AG +  D+  D LG+DQ+GN V+L+DIWPS  EI + +  N+++  F + Y  +F+GD  W A+  TES+ ++W  DSTYI+NPPYF GM+ +   I  I  ARVLA LGDS+TTDHISPAG+   DSPAG YL E+GV + +FNSYGSRRGNH+VMMRGTFAN+RLRN+LAPGTEGGWTR+QP + EMSI+DASMKYQ  G PL+VI G EYGTGSSRDWAAKGT LLGVKAV+ +SYERIHRSNLVGMGV+PLQFK GES  ++ LTGKE+Y + GL+NG S+   + V+ E G  +   E+ VRIDTP+E +Y +HGGILHYVLR LA+
Sbjct:   32 ISRLPFSLKILLENLLRNENGIDVTVSDIEALCNWDPNAAPATEIAFTPSRVVLQDFTGVPAVVDLAAMRDAMKALGGDVQKINPLSPAELVIDHSIQVDKYGTMDSAEINTQIEFQRNMERYGFLKWGQTAFDTFKVVPPGNGIVHQVNLEYLARVIFGAEKNGELMAYPDTVVGTDSHTTMINGIGVLGWGVGGIEAEAAMLGQAISMLIPQVVGFKLTGKMQEGVTATDLVLTVVDMLREHGVVGKFVEFFGSGLQHLPLADRATIANMAPEYGATCGIFPIDNESLRYLTLSGRSEEQVNLVEAYAKAQGMFHDENSIDAEYTAVLELDMSTVVPSISGPKRPQDRIELTVAKETYNRHFKEFENGRAVKS--------VAVSSDRGNYDFTDGNIAVAAITSCTNTSNPAVMLGAGLLARNAAAKGLKVKPWVKTSLAPGSKVVSHYLNAAGVMDDLETLGFNVVGFGCTTCIGNSGPLDPALSKAINDNDLIATSVLSGNRNFEGRIHADIQMNFLASPPLVVAYAIAGTMDFDLQNDSLGEDQEGNPVYLRDIWPSNHEIADTIQNNVSKTSFSEGYEGIFEGDANWQAVKTTESELFDWQGDSTYIKNPPYFDGMTLEVGTIDNISGARVLAKLGDSVTTDHISPAGAIAEDSPAGSYLKEKGVPKVNFNSYGSRRGNHEVMMRGTFANIRLRNQLAPGTEGGWTRHQPSNEEMSIFDASMKYQAEGTPLLVIGGTEYGTGSSRDWAAKGTNLLGVKAVLVQSYERIHRSNLVGMGVIPLQFKAGESADTYDLTGKEVYDVSGLKNGESKTAQITVHREDGSTVS-FEVDVRIDTPKEVEYCRHGGILHYVLRNLAS 887          

BLAST of aconitate hydratase vs. nr
Match: gi|1148670134|ref|WP_077410279.1| (aconitate hydratase AcnA [Marinicella sp. F2])

HSP 1 Score: 1215.68 bits (3144), Expect = 0.000e+0
Identity = 581/865 (67.17%), Postives = 689/865 (79.65%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENG-SRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLAN 972
            I+ LPFSLKILLENLLR E+G+DV+  DI+A+ NWD KA P+ EIAFTP+RVV+QDFTGVPA+VDLAAMRDA+  LGGDVQKINPL+PAELVIDHS+ VD +GT  A + N ++EF+RN ERY FL+WGQ+AFD+FKVVPP  GIVHQVN+EYLAR +F  E+NGE+ AYPDT+VGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQAI+ML+PQVVGFKL GK+ EG TATDLVLTV  MLR  GVVGKFVEF+G GL HLPLADRATIANMAPEYGATCGIFP+D E+L YLRLTGR ++Q+ LV+ YA AQGMF  E   +A YTA LELDM TVVPS++GPKRPQDRI LT       ++   +   R  K+        I +      +   DG + +AAITSCTNTSNP V++ +GLLARNA AKGL VKPWVKTSLAPGSKVV+ YL  A LM DL+ LGF+VVG+GCTTCIGNSGPL  A+  A+ + DL  +SVLSGNRNFEGRIHADI+MNFLASPPLVVAYA+AG +  D++ D LG+DQ G+ V+LKDIWPS  EI + +  N+NQ  F + Y  +F+GD  W A+  TES+ ++W  DSTYI+NPPYF GM+ D   I  IK ARVLA LGDS+TTDHISPAG+  ADSPAG YL E+GV + +FNSYGSRRGNH+VMMRGTFAN+RLRN+LAPGTEGGWTR+QP D EMSI+DASMKYQ    PL+VI G EYGTGSSRDWAAKGT LLGVKAV+ +SYERIHRSNLVGMGV+PLQFK GES  ++ LTGKE Y I+GL+NG S+   + V+ + G  +   E+ VRIDTP+E +Y +HGGILHYVLR LA+
Sbjct:   32 ISRLPFSLKILLENLLRHEDGIDVTVDDIEALCNWDPKAEPSTEIAFTPSRVVLQDFTGVPAVVDLAAMRDAMKALGGDVQKINPLSPAELVIDHSIQVDKYGTNDAADLNTQIEFQRNMERYGFLKWGQTAFDSFKVVPPGNGIVHQVNLEYLARVIFGAEQNGELMAYPDTVVGTDSHTTMINGIGVLGWGVGGIEAEAAMLGQAISMLIPQVVGFKLTGKLQEGVTATDLVLTVVDMLREHGVVGKFVEFFGAGLQHLPLADRATIANMAPEYGATCGIFPVDNESLRYLRLTGRSEEQVNLVEAYAKAQGMFHDEHSLDAEYTAVLELDMSTVVPSISGPKRPQDRIELTVAKATYNQHFTEFENGRPVKS--------IPVKSAKGDYEFTDGNIAVAAITSCTNTSNPAVMLGAGLLARNAAAKGLKVKPWVKTSLAPGSKVVSHYLNSAGLMDDLESLGFNVVGFGCTTCIGNSGPLDPALSKAINDNDLIATSVLSGNRNFEGRIHADIQMNFLASPPLVVAYAIAGTMDFDLYNDALGKDQDGHPVYLKDIWPSDHEIADTIGNNVNQNSFAQGYEGIFEGDANWEAVKTTESELFDWQDDSTYIKNPPYFAGMTLDVGTIDNIKGARVLAKLGDSVTTDHISPAGAIAADSPAGLYLQEKGVEKANFNSYGSRRGNHEVMMRGTFANIRLRNQLAPGTEGGWTRHQPSDEEMSIFDASMKYQAEDTPLLVIGGTEYGTGSSRDWAAKGTNLLGVKAVLVQSYERIHRSNLVGMGVIPLQFKAGESADTYDLTGKETYDIDGLKNGASKTAQVTVHRDDGSTVT-FEVDVRIDTPKEVEYCRHGGILHYVLRDLAS 887          

BLAST of aconitate hydratase vs. nr
Match: gi|493969552|ref|WP_006912778.1| (aconitate hydratase AcnA [Salinisphaera shabanensis] >gi|543109851|gb|ERJ18122.1| Aconitase protein [Salinisphaera shabanensis E1L3A])

HSP 1 Score: 1214.13 bits (3140), Expect = 0.000e+0
Identity = 606/886 (68.40%), Postives = 700/886 (79.01%), Query Frame = 0

Query:  109 IATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQDGNT---------------------FTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIKRIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            IA LP++ KILLENLLR E+G +V   DI+A+ NWDAKA P+ EIAFTPARVV+QDFTGVPA+VDLAAMRDA+  LGG   KINPL+PAELVIDHSVMVD +GT +A + NA+LEF RN ERY FLRWGQ AFDNFKVVPP TGIVHQVN+EYLAR VF  E+     AYPDTLVGTDSHTTM+NG+GVLGWGVGGIEAEAAMLGQ ITML+PQVVGFKL GK+ EG TATDLVLTVT+MLR  GVVGKFVEF+G+GLA LPLADRATIANMAPEYGATCGIFP+D ET+ Y+ LTGR  +Q+ELV+ YA AQG++R E +P+A YT  LELDM TV PSLAGPKRPQDR+ L DM +     V  + K R  KA D   KS  E  Q                         F L DG+V IAAITSCTNTSNP V++ +GLLARNA+ +GL VKPWVKTSLAPGSKVVT+YLEKA L  DLDKLGF +VGYGCTTCIGNSGPLPE IG AVR  +L V+SVLSGNRNFEGR+H D++MNFLASPPLVVAYAL+G+I +DM+ DPLGQD  GNDV+L+DIWPSQKEI + +  ++N  MFK SY +VF GD RW  + + E + ++W+  STY+QNPPYF GMS D +DI  I+ AR LA LGDSITTDHISPAG+   DSPAG+YL E+GV+  DFNSYGSRRGNH+VMMRGTFANVRLRN LAPGTEGGWTR+QP   EM IYDA+MKY ++  PL+V+AGKEYGTGSSRDWAAKGTLLLGVK V+ +S+ERIHRSNLVGMGVLPLQFKEGE+  S GL G E + IEGLE+G+  V +    ESG +       VRIDTP+EWDYYQ+GGILHYVLR+LA +
Sbjct:   33 IAKLPYAQKILLENLLRHEDGSNVDADDIKALANWDAKAEPDTEIAFTPARVVLQDFTGVPAVVDLAAMRDAMANLGGSPDKINPLSPAELVIDHSVMVDEYGTDKAFDLNAKLEFNRNKERYAFLRWGQGAFDNFKVVPPDTGIVHQVNLEYLARVVFGNEDTN--LAYPDTLVGTDSHTTMINGVGVLGWGVGGIEAEAAMLGQPITMLIPQVVGFKLTGKLAEGCTATDLVLTVTEMLRAKGVVGKFVEFFGDGLADLPLADRATIANMAPEYGATCGIFPVDGETIRYMELTGRPAEQLELVEAYAKAQGLWREEGEPDADYTDVLELDMSTVQPSLAGPKRPQDRVLLADMQKTYRREVEPFIKARAEKA-DPEDKSMAEAKQQSEAGLTSDDIGGPVHAPVSYKETEFDLHDGSVVIAAITSCTNTSNPAVMIGAGLLARNAIQRGLQVKPWVKTSLAPGSKVVTEYLEKAGLNVDLDKLGFQLVGYGCTTCIGNSGPLPEPIGEAVREHNLNVASVLSGNRNFEGRVHGDVRMNFLASPPLVVAYALSGSIDIDMNNDPLGQDADGNDVYLRDIWPSQKEIYDTIGTSLNSEMFKDSYGDVFAGDSRWKGLDVPEGEIFDWDETSTYVQNPPYFEGMSVDVADIPTIQGARCLALLGDSITTDHISPAGAITKDSPAGQYLQEKGVSPSDFNSYGSRRGNHEVMMRGTFANVRLRNLLAPGTEGGWTRHQPSGDEMFIYDAAMKYADDKTPLVVLAGKEYGTGSSRDWAAKGTLLLGVKTVIAQSFERIHRSNLVGMGVLPLQFKEGENAESLGLDGTETFDIEGLESGATEVTVKATKESG-DTSEFTAKVRIDTPKEWDYYQNGGILHYVLRQLAKA 914          

BLAST of aconitate hydratase vs. nr
Match: gi|1356006784|ref|WP_105189370.1| (aconitate hydratase AcnA [Pseudoalteromonas sp. T1lg48])

HSP 1 Score: 1209.51 bits (3128), Expect = 0.000e+0
Identity = 579/892 (64.91%), Postives = 703/892 (78.81%), Query Frame = 0

Query:  104 PDQDKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSA---------------------IEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIK-RIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            P  DK   LPFSLKILLENLLR E+G  ++K DIQA+++W  +A P+ E+AFTPARVVMQDFTGVPAIVDLAAMRDA+ +LGGD  KINPL+PAELVIDHSV VD +G   A + NA+LE++RN ERYEFLRWGQ+AFDN  VVPP TGIVHQVN+EYLAR VF K+E G   AYPD+LVGTDSHTTM+NGLG+LGWGVGGIEAEAAMLGQ I++L+PQVVGFKLKG +PEG TATDLVLTVTQ+LR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCGIFPID+ET+ YL+LT R D+Q+ L++ YA  QGM+R E   E  Y+  +ELD+GTVVPSLAGPKRPQDRI L    +   E++RA+  +R  K    + + A                      ++  +G  F L DGA  IAAITSCTNTSNP V++A+GL+A+ A   GL VKPWVKTSLAPGS+VVTDYL+KA LM DL+ LGF++VGYGCTTCIGNSGPLP+ I  A+    L VSS+LSGNRNFEGRIH D+KMNFLASPPLVVAYALAG   +D++ +PL QD QG DV+LKD+WPS  E+ E+V + + + MF KSY++V+KGDERW  I + ++ RY W+  STYI+  P+F GMSK+P  +  I  AR LA LGDS+TTDHISPAG+  AD+PAG+YL + GV++ DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGG TR QPED   SIYDA+M+YQ+N  PL+V+AG EYGTGSSRDWAAKG+LLLG+KAV+ +SYERIHRSNL+GMGVLPLQFK+G+     GLTGKE + I GL + ++ +++V  +E G++ K R++  VRIDTP+EW+YY+HGGIL YVLR++  S
Sbjct:   25 PLGDKAKRLPFSLKILLENLLRHEDGESINKDDIQALLDWQPQAKPSKEVAFTPARVVMQDFTGVPAIVDLAAMRDAMEQLGGDPSKINPLSPAELVIDHSVQVDSYGNNGAFDLNAKLEYQRNKERYEFLRWGQTAFDNLTVVPPATGIVHQVNLEYLARVVFEKDEQGHTFAYPDSLVGTDSHTTMINGLGILGWGVGGIEAEAAMLGQPISLLIPQVVGFKLKGALPEGTTATDLVLTVTQILRQKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGIFPIDQETIEYLKLTNRSDEQVALIEAYAKHQGMWR-EAGDEPLYSDIVELDLGTVVPSLAGPKRPQDRIPLDTAGDTITEHLRAFQDERAEKKALEMEEEARIVSEGGQPKQLQTEPEVTGIAKVKYNGEEFELSDGACVIAAITSCTNTSNPSVILAAGLVAKKAKELGLNVKPWVKTSLAPGSQVVTDYLKKAELMDDLEALGFNLVGYGCTTCIGNSGPLPDEIQQAITEHKLVVSSILSGNRNFEGRIHQDVKMNFLASPPLVVAYALAGRTDIDVYNEPLTQDVQGRDVYLKDLWPSVSEVAELVKSTVTKEMFAKSYADVYKGDERWRNIAVPQAKRYEWDEASTYIRKAPFFDGMSKEPPGLPSITGARCLAKLGDSVTTDHISPAGAIKADAPAGQYLIKHGVSQGDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGITRTQPEDDLASIYDAAMQYQKNNTPLVVLAGTEYGTGSSRDWAAKGSLLLGIKAVIAKSYERIHRSNLIGMGVLPLQFKDGQGHEELGLTGKETFDINGLCDKAKELEIVATNEDGQQTKFRVD--VRIDTPKEWEYYKHGGILQYVLRQMLRS 913          

BLAST of aconitate hydratase vs. nr
Match: gi|1356563504|ref|WP_105265156.1| (aconitate hydratase AcnA [Pseudoalteromonas sp. T1lg76])

HSP 1 Score: 1205.28 bits (3117), Expect = 0.000e+0
Identity = 579/889 (65.13%), Postives = 701/889 (78.85%), Query Frame = 0

Query:  107 DKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVLTKSAIEITQ---------------------DGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIK-RIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            DK   LPFSLKILLENLLR E+G ++ K DIQA+++W  +A P+ E+AFTPARVVMQDFTGVPAIVDLAAMRDA+ +LGGD  KINPL+PAELVIDHSV VD +G   A + NA+LE++RN ERYEFLRWGQ+AFDN  VVPP TGIVHQVN+EYLAR VF  EE G   AYPD+LVGTDSHTTM+NGLG+LGWGVGGIEAEAAMLGQ I++L+PQVVGFKLKG +PEG TATDLVLTVTQMLR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCGIFPID+ET+ YL+LT R D+QI L++ YA  QGM+R E   E  Y+ T+ELD+GTVVPSLAGPKRPQDRI L +  +  + ++R +  +R  K    +   A  +++                     +G  F L DGA  IAAITSCTNTSNP V++A+GL+A+ A   GL VKPWVKTSLAPGS+VVTDYL+KA LM DL+ LGF++VGYGCTTCIGNSGPL + I  A+ +  L VSS+LSGNRNFEGRIH D+KMNFLASPPLVVAYA+AG   +D++ +PL QD+QG DV+LKD+WPS  E+ E+V + + + MF KSY++V+KGDERW  I + E+ RY W+  STYI+  P+F GMSK+P  +  I  AR LA LGDS+TTDHISPAG+  AD+PAG+YL E GV++ DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGG TR QPED   SIYDA++ YQ+N  PL+V+AG EYGTGSSRDWAAKG+LLLG+KAV+ +SYERIHRSNL+GMGVLPLQFK GE     GLTGKE + I GL + ++ +++V  +E G++ K R++  VRIDTP+EW+YY+HGGIL YVLR++  S
Sbjct:   28 DKAKRLPFSLKILLENLLRHEDGENIHKDDIQALLDWQPQAEPSKEVAFTPARVVMQDFTGVPAIVDLAAMRDAMEQLGGDPSKINPLSPAELVIDHSVQVDSYGNNGAFDINAKLEYQRNKERYEFLRWGQTAFDNLTVVPPATGIVHQVNLEYLARVVFENEEQGHTFAYPDSLVGTDSHTTMINGLGILGWGVGGIEAEAAMLGQPISLLIPQVVGFKLKGALPEGTTATDLVLTVTQMLREKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGIFPIDQETIEYLKLTNRSDEQIALIEAYAKHQGMWR-EAGDEFLYSDTVELDLGTVVPSLAGPKRPQDRIPLDEAGKIISAHLRHFEDERAEKTALGMEDEARIVSEGGQPRQSKTAANTCGIAKVKFNGEEFELSDGACVIAAITSCTNTSNPSVILAAGLVAKKAKELGLNVKPWVKTSLAPGSQVVTDYLKKAGLMDDLEALGFNLVGYGCTTCIGNSGPLADEIHQAIIDHKLIVSSILSGNRNFEGRIHQDVKMNFLASPPLVVAYAIAGRTDIDVYNEPLTQDEQGRDVYLKDLWPSVSEVAELVKSTVTKEMFAKSYADVYKGDERWRTIAVPEAKRYQWDEASTYIRKAPFFDGMSKEPPGLPSIAGARCLAKLGDSVTTDHISPAGAIKADAPAGEYLLEHGVSQGDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGITRTQPEDKLTSIYDAAIHYQKNDTPLVVLAGAEYGTGSSRDWAAKGSLLLGIKAVIAKSYERIHRSNLIGMGVLPLQFKAGEGHEELGLTGKETFDINGLCDKAKELEIVATNEDGQQTKFRVD--VRIDTPKEWEYYKHGGILQYVLRQMLRS 913          

BLAST of aconitate hydratase vs. nr
Match: gi|1356021809|ref|WP_105199946.1| (aconitate hydratase AcnA [Pseudoalteromonas sp. T1lg10])

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 580/891 (65.10%), Postives = 702/891 (78.79%), Query Frame = 0

Query:  107 DKIATLPFSLKILLENLLRTENGVDVSKADIQAIINWDAKATPNDEIAFTPARVVMQDFTGVPAIVDLAAMRDAVVKLGGDVQKINPLAPAELVIDHSVMVDYFGTPQALEKNAELEFERNAERYEFLRWGQSAFDNFKVVPPRTGIVHQVNIEYLARTVFTKEENGEVTAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQAITMLVPQVVGFKLKGKMPEGATATDLVLTVTQMLRNLGVVGKFVEFYGEGLAHLPLADRATIANMAPEYGATCGIFPIDEETLTYLRLTGREDDQIELVKEYAMAQGMFRTEDQPEARYTATLELDMGTVVPSLAGPKRPQDRIALTDMSEKTAEYVRAYAKDRDAKAGDVL-----------------------TKSAIEITQDGNTFTLEDGAVTIAAITSCTNTSNPYVVVASGLLARNAVAKGLTVKPWVKTSLAPGSKVVTDYLEKANLMGDLDKLGFDVVGYGCTTCIGNSGPLPEAIGNAVRNGDLTVSSVLSGNRNFEGRIHADIKMNFLASPPLVVAYALAGNIQVDMHKDPLGQDQQGNDVFLKDIWPSQKEIQEVVTANINQAMFKKSYSNVFKGDERWAAIPITESDRYNWNSDSTYIQNPPYFVGMSKDPSDIKPIKNARVLAFLGDSITTDHISPAGSFNADSPAGKYLTEQGVARKDFNSYGSRRGNHQVMMRGTFANVRLRNELAPGTEGGWTRYQPEDSEMSIYDASMKYQENGVPLIVIAGKEYGTGSSRDWAAKGTLLLGVKAVVTESYERIHRSNLVGMGVLPLQFKEGESKVSHGLTGKEIYSIEGLENGSRHVDLVVNDESGKEIK-RIELLVRIDTPREWDYYQHGGILHYVLRRLANS 973
            DK   LPFSLKILLENLLR E+G ++ K DIQA+++W  +A P+ E+AFTPARVVMQDFTGVPAIVDLAAMRDA+ +LGGD  KINPL+PAELVIDHSV VD +G   A + NA+LE++RN ERYEFLRWGQ+AFDN  VVPP TGIVHQVN+EYLAR VF  EE G   AYPD+LVGTDSHTTM+NGLG+LGWGVGGIEAEAAMLGQ I++L+PQVVGFKLKG +PEG TATDLVLTVTQMLR  GVVGKFVEFYG+GLA LPLADRATIANMAPEYGATCGIFPID+ET+ YL+LT R D+QI L++ YA  QGM+R E   E  Y+ T+ELD+GTVVPSLAGPKRPQDRI L +  +  + ++R +  +R  +  +VL                       T    ++  +G  F L DGA  IAAITSCTNTSNP V++A+GL+A+ A   GL VKPWVKTSLAPGS+VVTDYL+KA LM DL+ LGF++VGYGCTTCIGNSGPL + I  A+ +  L VSS+LSGNRNFEGRIH D+KMNFLASPPLVVAYA+AG   +D++ +PL QD+QG DV+LKD+WPS  E+ E+V + + + MF KSY++V+KGDERW  I + E+ RY W+  STYI+  P+F GMSK+P  +  I  AR LA LGDS+TTDHISPAG+  AD+PAG+YL E GV++ DFNSYGSRRGNH+VMMRGTFANVRLRN+LAPGTEGG TR QPED   SIYDA+M+YQ+   PL+V+AG EYGTGSSRDWAAKG+LLLG+KAV+ +SYERIHRSNL+GMGVLPLQFK GE     GLTGKE + I GL + ++ +++V  +E G++ K R++  VRIDTP+EW+YY+HGGIL YVLR++  S
Sbjct:   28 DKAKRLPFSLKILLENLLRHEDGENIHKDDIQALLDWQPQAEPSKEVAFTPARVVMQDFTGVPAIVDLAAMRDAMEQLGGDPSKINPLSPAELVIDHSVQVDSYGNNGAFDINAKLEYQRNKERYEFLRWGQTAFDNLTVVPPATGIVHQVNLEYLARVVFENEEQGHTFAYPDSLVGTDSHTTMINGLGILGWGVGGIEAEAAMLGQPISLLIPQVVGFKLKGALPEGTTATDLVLTVTQMLREKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGIFPIDQETIEYLKLTNRSDEQIALIEAYAKHQGMWR-EAGDEFLYSDTVELDLGTVVPSLAGPKRPQDRIPLDEAGKIISAHLRHFEDERAER--NVLGMEDEARIVSEGGQPRQSKTAANTCGIAKVKFNGEEFELSDGACVIAAITSCTNTSNPSVILAAGLVAKKAKELGLNVKPWVKTSLAPGSQVVTDYLKKAGLMDDLEALGFNLVGYGCTTCIGNSGPLADEIHQAIIDHKLIVSSILSGNRNFEGRIHQDVKMNFLASPPLVVAYAIAGRTDIDVYNEPLTQDEQGRDVYLKDLWPSVSEVAELVKSTVTKEMFAKSYADVYKGDERWRTIAVPEAKRYQWDEASTYIRKAPFFDGMSKEPPGLPSIVGARCLAKLGDSVTTDHISPAGAIKADAPAGEYLLEHGVSQGDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGITRTQPEDKLASIYDAAMQYQQTDTPLVVLAGAEYGTGSSRDWAAKGSLLLGIKAVIAKSYERIHRSNLIGMGVLPLQFKAGEGHEELGLTGKETFDINGLCDKAKELEIVATNEDGQQTKFRVD--VRIDTPKEWEYYKHGGILQYVLRQMLRS 913