BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000004404 (supercontig:LSalAtl2s:LSalAtl2s231:1078605:1080545:-1 gene:EMLSAG00000004404 transcript:EMLSAT00000004404 description:"snap_masked-LSalAtl2s231-processed-gene-10.3")

HSP 1 Score: 311.227 bits (796), Expect = 5.509e-97
Identity = 213/564 (37.77%), Postives = 298/564 (52.84%), Query Frame = 0

Query:   28 FSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSG--DIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDA--RELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTG-----HEDQKRTNKIRTSKQAWLRESWFTFNVSQ--TAESV----KDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSPS 576
             SS  ++ +L+++E  F   + S    L      F K ++  YP+  +     I  E Y  +P+NA  +  R G  +Q L LK  +++ +         N +  FPT+ ++ E  S + LL EAY LN ++         GK  +   ++      L +IGI + N+GW D G++ ++ + +   G+   + +  K+++    I HDKLL  RG  GK HR F  PF  +LRKKKK+KKI               +    +    AL H+ CR   +   S E     RC  +H+ NPF +L PFKMEL++E PF+  F DFM + E +HF   A   L RS TG        +++   IRTSKQ WL ES  TFN+SQ  T E +    K    P  P N   ++ +++  GF   KRI  AT L  +  +SAEA+Q+ANYGLGG Y +H D++G+F  E   V    +F +    GDRLATFM YL DV  GG TVFP + I +   +G A+FWINL S G  D+ T HGGCPVL+GSKWITNKWI+SN Q    PCPL  S
Sbjct:   51 LSSTHQIAQLFELETKFVESLKS----LPLQEPEFHKTLNSIYPDTFLKKLKSIKGEDYASHPINALNLARRTG--WQSLQLKPWISNYTY------FLNYTNYFPTVDDYYEACSGLSLLIEAYQLNLTEFCK-----LGKIGEHMSLIPPGWSILTDIGISSINRGWXDTGITMMQHSLQAYSGKKEEY-QIYKKELDKSIIMHDKLLQLRGPIGKRHRTFRVPFSKELRKKKKYKKILKTGEDIRYRPTIEDNYESLKYKDSALFHKHCRKKEKVLESHE-----RCYLIHNDNPFYKLLPFKMELLSESPFIVIFRDFMSDVETKHFTDIAGRRLRRSQTGSLTEVEGKEEKGELIRTSKQIWLXES--TFNISQFLTEEEIQMITKGTGRPHFPGNYKDHLVILNEIGFRITKRIGRATKLNVEDAWSAEAYQIANYGLGGQYDVHFDARGHF--EYPPVDEITRFGYENY-GDRLATFMVYLRDVPMGGATVFPKINIYSNAQRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKWITNKWIASNVQMHLYPCPLKLS 586          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000012413 (supercontig:LSalAtl2s:LSalAtl2s909:41472:46417:1 gene:EMLSAG00000012413 transcript:EMLSAT00000012413 description:"augustus_masked-LSalAtl2s909-processed-gene-0.4")

HSP 1 Score: 248.054 bits (632), Expect = 6.546e-74
Identity = 180/572 (31.47%), Postives = 280/572 (48.95%), Query Frame = 0

Query:    8 LIVIVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKS-FQSDHILDARELVEIGIGACNQGWWDNGLSWLKIA-QKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQ-FGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHT-LFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKI-RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLS 574
            L +I+ S+   +   ++ +LFSS   +E LY+ E+  ++DI  +   LE    + +  +    P      D+  E +  NP++A  ++ R   ++  + + + L   +      +L  +   FP        ++ I LLQE Y L+  D +NGIIQ +  +    D  L   ++  +G  + N+G++D  + W+  A  K +K +D +    L + + +    HD+ L++RG   G D R +  PF+ +L KKKK+K+           K   +    ++   +    + +CR      RSP  + QL+  +LHH++P+L+L PFK+E+ N +P +    DF+ + E E ++ YA+  L RS +   D  R   + RTSKQ WL E              K++ I  I                   KRI++ATGL  +  +  E +QVANYGLGG Y  H+DS G    E               +GDR+AT MAYLSDV AGG T FP LG    P KG+A+FW N+   G +D    HGGCPVL GSKWITNKWI     ++   C +S
Sbjct:    9 LCIIIDSVVTLSENSEDGELFSSARAIESLYKKEIQINKDIELYINNLEEVLLSLKSTMKSITP------DVRKEIHPNNPISATMVLKRCALDWIKITVLTKLIFEN-----ERLLEELSQFPKEKEMYGAMNGIFLLQETYDLDPLDFSNGIIQNKYMTPITVDIQLKIDDMEYLGKLSYNRGYYDRAVDWIMAALMKNNKNKDSSNYIRLNKTLSTLMRAHDEFLEKRGSPAGSDWRTYVTPFNERLAKKKKYKRALKKNNGNRRYKMVPILGRRLNPREKWEQFNRLCRG--ENLRSPSDEVQLKSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAEDKLSRSFSLSNDGNRQVTLTRTSKQTWLFE--------------KNLTISKI-----------------ITKRIELATGLVAETLYGNEPYQVANYGLGGVYNSHMDSGGTIMPEFNE------------LGDRVATVMAYLSDVGAGGGTTFPSLGXKVTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSKWITNKWIRQLSNWEKHSCTMS 524          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000012326 (supercontig:LSalAtl2s:LSalAtl2s8:1477583:1479313:1 gene:EMLSAG00000012326 transcript:EMLSAT00000012326 description:"augustus_masked-LSalAtl2s8-processed-gene-15.1")

HSP 1 Score: 183.726 bits (465), Expect = 6.116e-51
Identity = 134/506 (26.48%), Postives = 227/506 (44.86%), Query Frame = 0

Query:   76 SGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSL--LFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQK-PFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRH-----NTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPL 573
            S D   E    NP++A+ +V R   +++ L  + +  D   P+L+ K+    L  + P   +      ++  + + Y L+  +L+ GII+ R  S +    L A++ + +G  + N G     L W + A               K  + +  +  D++ +      ++H          LR+ +    + +   + ++ K                   +CR      R P++   ++C     ++P+  L P  +E VN  P +  ++D + +NEM+  +  A+P L RS    E     +K RTSK  WL++S                      F+P     +V +  F    ++   TGL +      AE  Q+ANY  GGHYQ H D        + +   P++  +     +  +GDR+AT+M Y+++V +GG TVFP LG+  +P  G+A+FW NL+ +G+ D  T HG CPVLLGSKW++NKWI    Q  T PC L
Sbjct:   49 SDDDYFERISENPIHAYKLVKRFSVDWKKLEPEVIEDD--WPELELKIHKKKLSTIIPKEQDLHGSAQALLRISDVYDLDVRNLSEGIIENRETSAK----LSAKDCLFMGKHSFNSGELSRSLEWFEQAW---------LLAGXKPNLXATTLSRDEVRNFLEYASQEH---------DLRRDQLRMNVPSPRSEEDMFK------------------SLCRG--EEHREPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYLMRSQVQSETYSEVSKTRTSKTGWLQDS----------------------FHP-----IVSKMSF----KVNSMTGLNSDTWKEDAELLQIANYINGGHYQPHHDY-------VIKGVQPDKMIYGNKGIDYYIGDRIATWMVYMNNVISGGRTVFPRLGLGVEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSKWVSNKWIREGGQMFTRPCGL 472          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000004330 (supercontig:LSalAtl2s:LSalAtl2s227:607006:610705:1 gene:EMLSAG00000004330 transcript:EMLSAT00000004330 description:"maker-LSalAtl2s227-augustus-gene-6.6")

HSP 1 Score: 165.622 bits (418), Expect = 3.559e-44
Identity = 131/455 (28.79%), Postives = 212/455 (46.59%), Query Frame = 0

Query:  145 LLQEAYALNCSDLANGIIQVRGKSFQ--SDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPK-LRKKKKFKKI-----------SANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISA--QPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSPSQGNYPVF 583
            +L + Y  +   +  GI++ +  S +      L  ++L+ +   A +  W+D+ ++ L++  K     DP+ T++ + K+   +I++  L     + GK+ + +    D + L+KKKK  K+           S  P  A+L    L +  ++      L+ E  R   R   SP   ++ +C  LHH++P+LRL PFK E+++E P     H      +++  Q Y    L         +   NK   S   W +E        +  E +   +I     +P+ Y           + +I+ AT L+    +S+ A+QV NYGLGG  +LHID  G   G +        F+     GD + T M +L DV+AGG T F   G     +P KGSA  W NL   GK+   T+HGGCP++ G+KWI NKW+   DQF   PC L+  + +Y VF
Sbjct:   99 VLLDTYKFDLRGMLQGIVRDQSLSIELLGKERLTLQDLLHLSKKAFDYNWYDSSIALLRVXLKAH--SDPS-TDSYREKLV--QIHNIHLKKNLKRVGKNFKVYPYMIDSQTLQKKKKQPKVIGKTTFYNRNVSHIPPPAQLDVQKLKESELAMIEFTDLAEEEFRRICREEISPPESSERKCILLHHNDPYLRLGPFKQEIISEDPPRMILHWL---KDIKFNQEYNLQIL---------RGELNKFDPSFPDWYKE--------KEPEDIYGFQI----VHPILY---------RLSLKIEHATRLKVTDRWSSSAYQVTNYGLGGLCELHIDPHGMLRGTMVNDDRVSLFK----TGDMILTLMGWLDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKWILNKWMFYFDQFHGYPCGLNEFE-DYKVF 510          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000011700 (supercontig:LSalAtl2s:LSalAtl2s812:79332:86643:-1 gene:EMLSAG00000011700 transcript:EMLSAT00000011700 description:"maker-LSalAtl2s812-augustus-gene-0.6")

HSP 1 Score: 167.933 bits (424), Expect = 3.274e-43
Identity = 157/577 (27.21%), Postives = 260/577 (45.06%), Query Frame = 0

Query:    6 VLLIVIVGSLGDWA--VKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYV----DLTYPEG--PMSGDID---IESYVGNPLNAFGMVGRLGTNFQDL--NLKSLLTDSSLPDLQR-KLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQP-FVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEA-------FQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWI 560
            +L+ V V +L  +   V  +N  L S    + R+++IE    + +    + L+ S +  ++Y      L   EG  P+   I+    E  VGNP+  + ++ RL  N+++   +LK +     + D++R K  N  L  P   + +    ++  + + Y +N ++ +NG I       Q+   L  R+ + +G  A N  +    L+WL  AQ +      +  EALK+               +    + +R  SK   PK    K    + A P + E       +L +S T R      +CR G +   S E +++L C + +     L+L+P K+E+ + +P  +  FHD    +E        + +L  +  G +  K  + +R S   W+                           P   + +VD+     +++I+   GL +      EA        QVANYGLGGHY  H DS   +        S E+ +   + GDR++TFM YLSDVQ GG+T FP LG++  P KGSA+FW N++ +G+ D    HG CPV++GSKW+ NKWI
Sbjct:   12 ILITVFVSTLISFPNPVISENSILNSE--SLYRVFKIERELFKVLQERQKQLQDSLELIKEYTRDVESLYRNEGCWPLESCIESKIFEQIVGNPIYNYQVLRRLIVNWKNTEDSLKKIDIKQIMTDIKRLKKQNGGL--PDGKDLQTAAKALNRIHKIYDINTTEFSNGNIL----GHQTSANLGLRDTLYLGRFAANTDYPGVALNWLNTAQSQANLHPNSSEEALKQ--------------VKLALRQVNRKLSK--KPKSMGDKDPYILGAIPDKTEDP-----NLVISNTDRRNF-EALCR-GEKLISSFE-ESKLYCYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSETHDLIESVRSDLTSAGVGTD--KTISDMRISSHVWI---------------------------PDGMLSIVDK----ISEKIENVIGLRSMAQLDPEAKKEEYEMLQVANYGLGGHYDCHHDSMFIYKEPQFIPKSVEETQSPYITGDRMSTFMIYLSDVQKGGNTAFPRLGVATSPRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSKWVANKWI 523          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000009563 (supercontig:LSalAtl2s:LSalAtl2s615:626046:628492:-1 gene:EMLSAG00000009563 transcript:EMLSAT00000009563 description:"maker-LSalAtl2s615-augustus-gene-5.47")

HSP 1 Score: 158.688 bits (400), Expect = 1.270e-41
Identity = 152/601 (25.29%), Postives = 254/601 (42.26%), Query Frame = 0

Query:   11 IVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLAN--DSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQD---------------PAFTEA-LKRKIK----------------SGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVT--GHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            +V + G   +   N+DL   I     + Q    + ++  S  + L+   + + KY+         S D   E +V NPLN+F ++ +L ++F D+    +   S+L      + +  +S  +PT  + +   S++  LQE Y +N  D+ NG  ++ G  +     L A +  E+G    N+G       W+  A  R   +D                A+ +  L+R +K                  ++Y++  + + G  G       +  D  L + K+      +    E       +L   +T R      +CR         + D  L+C      +PF+++AP K E +  +P +  FH+ + +NE+E  +  A P   R+        +  T   R SK AW++        S+  + VK +           Y +V D  G N          +ET     +E  QV+NYG+GGHY  H D          R    + F  +   G+R+AT++ Y S+V+ GG TVFP+L ++  P KGSA+FW NL   G+ +  T H   PVL G+K ++N WI    Q  T PC L+P
Sbjct:    1 MVATSGSDGIFTSNEDLRQXIYTKSVVLQXVEKYIKEEESRIQRLKGYVEQYNKYLG--------SADGHEEDFVSNPLNSFILIKKLTSDFNDV-YDLIKETSNLNKFSENITDSRESNKWPTDEDLKGAASALIRLQEVYKINTRDMRNG--RLNGVDYGPQ--LTAHDCFELGRQTYNEGDHYRTEIWMTEALSRYDEEDEKTVSKGDIYEYLGFSAYVQGHLRRALKFTNLLLKELPNHPRAIGNKLYYENAIMESGGSGPSLDGSQRKGDDGLDELKE------DAVLKEGTGSNAEELLYPETVRY---QRLCRGESN--EKKKFDRDLKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANPRFKRATVQNAKTGELETANYRISKTAWIK--------SEENDVVKGI-----------YQRVHDVTGLN----------MET-----SEELQVSNYGIGGHYDPHFD--------FARREEKDAF-SSLGTGNRIATWLFYESEVEQGGATVFPVLDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTKXVSNFWIHERGQEFTRPCALTP 534          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000005013 (supercontig:LSalAtl2s:LSalAtl2s260:584077:587279:-1 gene:EMLSAG00000005013 transcript:EMLSAT00000005013 description:"maker-LSalAtl2s260-snap-gene-6.18")

HSP 1 Score: 156.762 bits (395), Expect = 2.010e-41
Identity = 122/453 (26.93%), Postives = 214/453 (47.24%), Query Frame = 0

Query:   26 DLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEAL---KRKIKSGEIYHDKLLDQRGQ-FGKDHRCFSKPFDPKLRKKKKFK---KISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKI---RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQG 468
            +LFSS   + RL  +    +  I  + E L +       ++   Y E  ++ +   ++Y+ NP+NAF ++ R  T+ + L +   + + +  ++  +++      P   + E   + + LLQE Y L+    A           +++  L+  ++  +G  A N+  +D    WL I+  +   +D    E +   K+ IK+   YHD++L+ RG   G   R +  P +PKL  K K+K   K +      ++    L +L   + T     + +CR      R P  D  L C +LH ++P+L+L PFK+E +N  PF+  +HDF+++NE+E F+++++P L RS   H  +K +N++   RTSKQ WL +          AE+  +                V+      + RI  AT L  +     E +Q+ANYG+GG Y  H+DS G
Sbjct:   20 ELFSSSDSIGRLANVNSRLNNKIQIYLEELNQQISILDNFLSTYYDEYNLT-ESQADTYISNPINAFTLIKR--TSHEWLKIHDSIYNETSENMFTEISELLSGLPNSGDIEGAANGLFLLQETYNLDIDLFAK----------ENNAALNGNDMSLMGKLAFNKQVYDRAYEWL-ISSLKKAIEDNDSVETISQIKKDIKTVIKYHDQILETRGSPRGSTWRTYVNPLEPKLASKNKYKNALKANNGSMSYKMEYKLLTELKDYEITDQF--NALCRG--EDIRDPIDDKDLSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERS--QHSGKKNSNEVSFKRTSKQTWLVD----------AETQNN--------------NTVNTLANKISNRISYATKLAVKSKKGGEDYQIANYGMGGVYTPHMDSFG 428          

HSP 2 Score: 76.2554 bits (186), Expect = 8.995e-15
Identity = 38/89 (42.70%), Postives = 55/89 (61.80%), Query Frame = 0

Query:  637 LEAKMASHASAVRRQIKVLDNFLNTYYSDYNYTKEDAHEYVSNPINTYMLIKRTSLEWPQIKKVLFNDTLDRDYEEIVALSESINASND 725
            L  K+  +   + +QI +LDNFL+TYY +YN T+  A  Y+SNPIN + LIKRTS EW +I   ++N+T +  + EI  L   +  S D
Sbjct:   38 LNNKIQIYLEELNQQISILDNFLSTYYDEYNLTESQADTYISNPINAFTLIKRTSHEWLKIHDSIYNETSENMFTEISELLSGLPNSGD 126          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000004188 (supercontig:LSalAtl2s:LSalAtl2s221:321106:325044:-1 gene:EMLSAG00000004188 transcript:EMLSAT00000004188 description:"maker-LSalAtl2s221-augustus-gene-3.12")

HSP 1 Score: 160.614 bits (405), Expect = 2.014e-41
Identity = 151/599 (25.21%), Postives = 259/599 (43.24%), Query Frame = 0

Query:   26 DLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQD-----LNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLA-NGIIQVRGKSFQS-----DHI-LDARELVEIGIGACNQGWWDNGLSWLKIAQKR---DKGQDP----AFT--------EALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQT--------TRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAW-LRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPL---SPSQGNYPVFQR 585
            DLF+S++ ++ L+  +    +D     + LE  A+  R+YV  ++ +  +  +++I S++G+P+N++ ++  +   ++D     LN++ ++ +       +         P   + +     I  L   Y LN + L   G +      +Q+      H+ L+A ++  I   A +   ++ G   L +A K+   +K   P     F         + ++  +K     H+  L +RG     HR    P   K  KKK  KKIS N F   ++ + + DL   +          +  +   +  +GV+ +R+P+ D  LRC +    NP+L+L P K E++N  P+V  F + + + E ++   Y  P L         + ++N +   K  W ++ +W   + ++  E +            ++   V+D               L   K   ++ F   NYG+GGHY  H D   Y         S E+F+      +R++T M  L    AGG TVFP  G  + P KG+ I W NL+  G  D LT H  CPVL G KWI NKWI    Q    PC L   +   G  P F+R
Sbjct:  162 DLFTSLSNLKDLWINDQEVVQDFEDVAQNLESMAKHLRQYVK-SHKKNGLDSEVNI-SFLGHPINSYHLIKHVSMGWRDIVDNVLNVEKIVREK----FDKFHERGEKQLPDYYDIQGASFGIARLISLYELNATSLVREGRVVGELGDWQTIYSEPSHVSLNAWDVYSIAAEAFSNRLFNAGYDMLDLALKKLMEEKINPPNDQVVFNVTATHLEMDVVRSSLKKVIEMHNVFLKKRGNISSSHRTHDHPVGLKKPKKKSKKKISKNRFA--IMDNLIKDLETYENALKSEETYVKTPIQTNLLCSGVK-FRTPKEDADLRCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKL----DFPPGRMKSNSL---KNDWTMKNTWPVEDDNKVLEKLT-----------LRVEAVMDL--------------LANSKNNESDKFMCGNYGIGGHYGTHPDFSAY---------SHEEFKVPGSKVNRISTVMTILQSPSAGGATVFPYTGAYSYPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQKWIGNKWIGYTPQIYKKPCSLKMGTKLSGPLPKFKR 710          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000012938 (supercontig:LSalAtl2s:LSalAtl2s988:173248:175333:-1 gene:EMLSAG00000012938 transcript:EMLSAT00000012938 description:"snap_masked-LSalAtl2s988-processed-gene-1.22")

HSP 1 Score: 141.354 bits (355), Expect = 5.301e-36
Identity = 138/530 (26.04%), Postives = 232/530 (43.77%), Query Frame = 0

Query:   12 VGSLGDWAVKGDNDDL----FSSIAEVERLYQIEVGFSRDILSFTE--LLERSAQ-AFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRL--GTNFQDLNLKSLLTD--SSLPDLQRKLANDSLL--FPTLSNFEEVVSSIGLLQEAYALNCSDLAN-GIIQVR-GKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLK--IAQKRDKG--QDPAFTEA-----LKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRS-VTGHEDQKRTNKI---RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRH----NTVVGDRLATFMAYLSDVQAGGHT 509
            V   G   VK D + L    ++S+ E+ + ++ E  +  DI +  +  L++   Q +F  Y+  +Y +     D   E+++ NPLNA+ ++  +  G N  + +++         LP   RKL + S     P   + +   +++  L E Y  N +     G+I+ + G+ F++   L   ++ +IG+   N     +G+  +   +A+ +  G    P F ++     LK  +K+ +  HD+ LD+ G     H     P+D +L KKKKF K+ +        K  L + S+  T +    +    NG    RSP++   L CR+ H   P+ R  P K+E V+ +P +   HDF+ +   + F   A PNL RS + G ++   TN +   R S+Q+W+ E                 E+P                      +I     L       +E +QVANYG  GHY  H D       ++   +SP         N   GDR+AT M YLSDV+ GG+T
Sbjct:   20 VDGSGYTVVKEDTERLQKTIYTSMYEMSKFFEREKQYIEDIQTVIDKKLVDMETQGSFGAYL-ASYEDIVGLQDDSDEAFLNNPLNAYNLIRHVAVGWNVVESSIQRYKEKHPGQLPKRVRKLLSRSKREHLPGAEDLDGASTALVRLHEYYNFNITSFVEEGVIETKDGERFETTGELSVWDIFKIGVKGTNMMILGSGIEIMSHGLAKAKRDGLVTTPPFVDSHDFKTLKNIVKTAKTVHDQKLDRWGPLTPTHSTNYVPYDKRLAKKKKFNKVVSE-------KMILNNPSIIDTIQETKQYRKLCNG-EELRSPDVLKDLTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFISDEITKEFINSASPNLKRSAMRGFKNG--TNAVDDRRVSEQSWINE-----------------EVPA---------------ARKLTDQINDYLNLLVDSSIHSELYQVANYGTAGHYSSHYD-------QVLMGSSPNDLERRNIFNIKTGDRMATIMGYLSDVEEGGYT 499          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. L. salmonis genes
Match: EMLSAG00000010988 (supercontig:LSalAtl2s:LSalAtl2s73:416831:418075:1 gene:EMLSAG00000010988 transcript:EMLSAT00000010988 description:"maker-LSalAtl2s73-augustus-gene-4.34")

HSP 1 Score: 81.2629 bits (199), Expect = 6.194e-19
Identity = 37/69 (53.62%), Postives = 44/69 (63.77%), Query Frame = 0

Query:  497 MAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQ 565
            M YLSDV  GG T F  +     P KGS++FW NL S GK D+LT HG CP + G KW++NKWI S  Q
Sbjct:    1 MLYLSDVDGGG-TAFTKINKLVYPKKGSSVFWFNLYSDGKEDTLTIHGACPTMYGIKWVSNKWIRSFAQ 68          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|32171589|sp|Q20065.1|P4HA2_CAEEL (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor)

HSP 1 Score: 188.734 bits (478), Expect = 9.898e-51
Identity = 166/591 (28.09%), Postives = 265/591 (44.84%), Query Frame = 0

Query:    6 VLLIVIVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSS--LPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEI--YHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQA--------ELL--KHTLFDLSVSQTTRVALS--------HEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRH-NTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPL 573
            VLL+ ++  L        + DLF++IA+++ +   E   +  I  + E         R+Y          +  +  E +V NP+NA+ ++ RL T ++ +    L   +S  L ++        + FP   +     +++  LQ+ Y+L+  DL+NGII     S +    L   +  E+G  A NQ  + + L W+++A  + + ++P         I+  EI  Y    L Q+G   +            L   K+  KI+ N  +A        ++L  K  + DL      RV             +CR  + P   P+   +LRC +L    PFL+LAP K+E++   P    F + +H++E+E  +  A P L R+            ++ SK   L  +  T+ +S++A    D++             V+DR      +RI+  T L      ++E  QVANYGLGGHY  H D          R      F+  NT  G+R+AT + Y+S  + GG TVF  LG +  P+K  A+FW NL+  G+ D  T H  CPVLLG KW++NKWI    Q  T PC L
Sbjct:    4 VLLVCLLAGLA-------HADLFTAIADLQHMLGAEKDVTTIIDQYIEAERARLDDLRRYAHEYVHRNAHAESVGPE-FVTNPINAYLLIKRLTTEWKKVENIMLNNKASTFLKNITDNRVRSEVKFPGEEDLSGAATALLRLQDTYSLDTLDLSNGIIGGEKVSNK----LSGHDTFEVGRSAYNQKDYYHCLMWMQVALVKIENENPP-------TIEEWEILEYLAYSLYQQGNVRR-----------ALSLTKRLAKIAPNHPRAKGNVKWYEDMLQGKDMVGDLPPIVNKRVEYDGIVERDAYEALCRGEIPP-VEPKWKNKLRC-YLKRDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKRAT-----------VQNSKTGELEHA--TYRISKSAWLKGDLD------------PVIDR----VNRRIEDFTNLNQA---TSEELQVANYGLGGHYDPHFD--------FARKEEKNAFKTLNT--GNRIATVLFYMSQPERGGATVFNHLGTAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKWVSNKWIHEKGQEFTRPCGL 520          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|20455169|sp|O15460.1|P4HA2_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor)

HSP 1 Score: 183.726 bits (465), Expect = 5.573e-49
Identity = 149/518 (28.76%), Postives = 238/518 (45.95%), Query Frame = 0

Query:   80 DIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWL-KIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSK-----PFDPKLRK------------KKKFKKISANPFQAELLK-HTLFDLSVSQTTRVALSHEMCRN-GVRPWRSPEMDTQLRCRWLHHSN--PFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKR----TNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPC 571
            D E Y+ +P+NA+ +V RL T++    L+ L+   S       L+     FPT  +      ++  LQ+ Y L+   ++ G  ++ G  +Q+  +L   +   +G  A N+G + + + W+ ++ ++ D G++   T++      S  ++         Q G  HR           DP   +            +++ +K   N  +AEL     +++  V       +   +CR  GV+   +P    +L CR+ HH N  P L +APFK E   + P + +++D M + E+E  +  AKP L R+     D K         R SK +WL E                 +  P+       V  V+R       R++  TGL  +   +AE  QVANYG+GG Y+ H D          R    + F+H    G+R+ATF+ Y+SDV+AGG TVFP LG +  P KG+A+FW NL  +G+ D  T H  CPVL+G KW++NKW     Q    PC
Sbjct:   75 DAEGYLAHPVNAYKLVKRLNTDWP--ALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRG--ELPGTKYQA--MLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVF---------QLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVK--LTPRRQKRLFCRY-HHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATV--RDPKTGVLTVASYRVSKSSWLEE-----------------DDDPV-------VARVNR-------RMQHITGLTVK---TAELLQVANYGVGGQYEPHFD--------FSRNDERDTFKH-LGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPC 529          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|2498741|sp|Q60716.1|P4HA2_MOUSE (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor)

HSP 1 Score: 182.956 bits (463), Expect = 1.032e-48
Identity = 146/518 (28.19%), Postives = 235/518 (45.37%), Query Frame = 0

Query:   80 DIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWL-KIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSK-----PFDP-------------KLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRN-GVRPWRSPEMDTQLRCRWLHHSN--PFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKR----TNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPC 571
            D E Y+ +P+NA+ +V RL T++  L    L+   +       L+     FPT  +      ++  LQ+ Y L+   ++ G  ++ G  +Q+  +L   +   +G  A N+G + + + W+ ++ ++ D G++   T++L     S  ++         Q G  HR           DP             +L ++++ K +S         +  L++          +   +CR  GV+   +P    +L CR+ HH N  P L +APFK E   + P + +++D M + E+E  +  AKP L R+     D K         R SK +WL E                 +  P+       V  V+R       R++  TGL  +   +AE  QVANYG+GG Y+ H D          R    + F+     G+R+ATF+ Y+SDV+AGG TVFP LG +  P KG+A+FW NL  +G+ D  T H  CPVL+G KW++NKW     Q    PC
Sbjct:   77 DPEGYLAHPVNAYKLVKRLNTDWPALG--DLVLQDASAGFVANLSVQRQFFPTDEDESGAARALMRLQDTYKLDPDTISRG--ELPGTKYQA--MLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSYAVF---------QLGDLHRAVELTRRLLSLDPSHERAGGNLRYFERLLEEERGKSLSNQTDAGLATQENLYERPTDYLPERDVYESLCRGEGVK--LTPRRQKKLFCRY-HHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATV--RDPKTGVLTVASYRVSKSSWLEE-----------------DDDPV-------VARVNR-------RMQHITGLTVK---TAELLQVANYGMGGQYEPHFD--------FSRSDDEDAFKR-LGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPC 531          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|81870817|sp|Q6W3F0.1|P4HA3_MOUSE (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor)

HSP 1 Score: 181.8 bits (460), Expect = 3.317e-48
Identity = 161/581 (27.71%), Postives = 255/581 (43.89%), Query Frame = 0

Query:   17 DWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLT-YPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDL--NLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQ-VRGKSFQSDHI------LDARELVEIGIGACNQGWWDNGLSWLKIAQ---KRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRG------QFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRN-GVRP--WRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            D A     +D FS++  V R    E    R +L       R  +A  +  DLT + +  +S   D++  V NPL AF ++ RL ++++++  +L++     +L D   K+  D    P   + E    ++  LQ+ Y LN   LA G+ Q V G S    +       L A +  ++G  A + G + + + WL+ A    +R  G+     EA    ++    Y      Q G         ++   +S       R   K++++ A        +  +   +V           +C+  G +P  ++ P     L C +  +S+P+L L P + E+V+ +P +  +HDF+ + E +  +  A+P L RSV    +++   + R SK AWL+++                               VD        RI   TGL+ Q P+ AE  QV NYG+GGHY+ H D            TSP    +    G+R+ATFM YLS V+AGG T F     S    K +A+FW NL  +G+ D  T H GCPVL+G KW+ NKWI    Q    PC  +P
Sbjct:   18 DPAAATGREDTFSALTSVARALAPE----RRLLGTLRRYLRGEEA--RLRDLTRFYDKVLSLHEDLKIPVVNPLLAFTVIKRLQSDWRNVVHSLEATENIRALKDGYEKVEQD---LPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSSITDLYSPRQLFSLTADDCFQVGKVAYDTGDYYHAIPWLEEAVSLFRRAHGEWKTEDEA---SLEDALDYLAFACFQVGNVSCALSLSREFLVYSPDNKRMARNVLKYERLLAENGHQMAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIP----SLYCSYETNSSPYLLLQPARKEVVHLRPLIALYHDFVSDEEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDT-------------------------------VDPMLVTLDHRIAALTGLDIQPPY-AEYLQVVNYGIGGHYEPHFD----------HATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYGNFSVPVVKNAALFWWNLHRSGEGDGDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSTNP 540          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|75053350|sp|Q75UG4.1|P4HA3_BOVIN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor)

HSP 1 Score: 181.8 bits (460), Expect = 3.825e-48
Identity = 150/521 (28.79%), Postives = 232/521 (44.53%), Query Frame = 0

Query:   80 DIESYVGNPLNAFGMVGRLGTNFQDL--NLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSD-------HILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRC---FSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTT--RVALSHEMCRN---------GVRP--WRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            D  + V NPL AF ++ RL ++++++  +L++     +L D   ++  D    P   + E    ++  LQ+ Y LN   LA G+ Q    S  +D         L   +  ++G  A + G + + + WL+ A    +G   ++ E       S E   D L     Q G +  C    S+ F   L      K+++ N  + E L     + +V++T   R  + H   R+         G +P  +R P     L C +   S+P+L L P + E+++ +P+V  +HDF+ + E +  +  A+P L RSV    +++   + R SK AWL+++                               VD        RI   TGL+ Q P+ AE  QV NYG+GGHY+ H D            TSP    +    G+R+ATFM YLS V+AGG T F     S    K +A+FW NL  +G+ D  T H  CPVL+G KW+ NKWI    Q    PC   P
Sbjct:   78 DSATPVSNPLLAFTLIKRLQSDWKNVVHSLEASENIRALKDGYERVEQD---LPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAVTDLYSPRRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRG---SYGEWKTEDEASLEDALDHLAFAYFQAG-NVLCALNLSREF---LLYSPDNKRVARNVLKYEKLLAESPNQAVAETVMQRPNVPHLQTRDTYEGLCQTLGSQPTHYRIP----SLYCSYETSSSPYLLLQPVRKEVIHLEPYVVLYHDFVSDAEAQTIRGLAEPWLQRSVVASGEKQLPVEYRISKSAWLKDT-------------------------------VDPVLVTLDHRIAALTGLDVQPPY-AEYLQVVNYGIGGHYEPHFD----------HATSPSSPLYRMNSGNRVATFMIYLSSVEAGGATAFIYGNFSVPVVKNAALFWWNLHRSGEGDGDTLHAACPVLVGDKWVANKWIHEYGQEFRRPCSSRP 542          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|74738714|sp|Q7Z4N8.1|P4HA3_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor)

HSP 1 Score: 181.03 bits (458), Expect = 5.551e-48
Identity = 148/521 (28.41%), Postives = 235/521 (45.11%), Query Frame = 0

Query:   80 DIESYVGNPLNAFGMVGRLGTNFQDL--NLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSD-------HILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRC---FSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTT--RVALSHEMCRN---------GVRP--WRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            D  + V NPL AF ++ RL ++++++  +L++     +L D   K+  D    P   + E    ++  LQ+ Y LN   LA G+ Q    S  +D         L   +  ++G  A + G + + + WL+ A    +G   ++ E       S E   D L     + G +  C    S+ F   L      K+++ N  + E L     +  V++    R  + H   R+         G +P  ++ P     L C +  +SN +L L P + E+++ +P++  +HDF+ ++E +  +  A+P L RSV    +++   + R SK AWL+++                               VD        RI   TGL+ + P+ AE  QV NYG+GGHY+ H D            TSP    +    G+R+ATFM YLS V+AGG T F    +S    + +A+FW NL  +G+ DS T H GCPVL+G KW+ NKWI    Q    PC  SP
Sbjct:   78 DSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQD---LPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRG---SYGEWKTEDEASLEDALDHLAFAYFRAG-NVSCALSLSREF---LLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIP----SLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDT-------------------------------VDPKLVTLNHRIAALTGLDVRPPY-AEYLQVVNYGIGGHYEPHFD----------HATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSP 542          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|81870816|sp|Q6W3E9.1|P4HA3_RAT (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor)

HSP 1 Score: 180.644 bits (457), Expect = 9.238e-48
Identity = 170/588 (28.91%), Postives = 263/588 (44.73%), Query Frame = 0

Query:   17 DWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLT-YPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDL--NLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQ-VRGKSFQSDHI------LDARELVEIGIGACNQGWWDNGLSWLKIAQ---KRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRC---FSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTT--RVALSHEMCRN---------GVRP--WRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            D A     +D FS++  V R    E    R +L       R  +A  +  DLT + +  +S   D++  V NPL  F ++ RL ++++++  +L++     +  D   K+  D    P   + E    ++  LQ+AY LN   LA G+ Q V G S    +       L A +  ++G  A + G + + + WL+ A    +R  G+     EA      S E   D L     Q G +  C    S+ F   L      K+++ N  + E L      L  ++T   R  + H   R+         G +P  ++ P     L C +  +S+P+L L P + E+++ +P V  +HDF+ + E +  +  A+P L RSV    +++   + R SK AWL+++                 + P+       +  +DR       RI   TGL+ Q P+ AE  QV NYG+GGHY+ H D            TSP    +    G+R AT M YLS V+AGG T F     S    K +A+FW NL  +G+ D  T H GCPVL+G KW+ NKWI    Q    PC  +P
Sbjct:   20 DPAAAAGREDTFSALTSVARALAPE----RRLLGTLRRYLRGEEA--RLRDLTRFYDKVLSLHEDLKIPVVNPLLVFTLIKRLPSDWRNVVHSLEATENIRAPKDGYEKVEQD---LPAFEDLEGAARALMRLQDAYMLNVKGLAQGVFQRVTGSSITDLYSPRQLFSLTADDCFQVGKVAYDTGDYYHAIPWLEEAVSLFRRSYGEWKTEDEA------SLEDALDYLAFACYQVG-NVSCALSLSREF---LVYSPDNKRMARNVLKYERLLAENGHLMAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIP----SLYCSYETNSSPYLLLQPARKEVIHLRPLVALYHDFVSDEEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDT-----------------VDPV-------LVTLDR-------RIAALTGLDIQPPY-AEYLQVVNYGIGGHYEPHFD----------HATSPSSPLYKMKSGNRAATLMIYLSSVEAGGATAFIYGNFSVPVVKNAALFWWNLHRSGEGDDDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCDTNP 542          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|82082587|sp|Q5ZLK5.1|P4HA2_CHICK (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor)

HSP 1 Score: 176.022 bits (445), Expect = 3.661e-46
Identity = 145/521 (27.83%), Postives = 234/521 (44.91%), Query Frame = 0

Query:   80 DIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKR-DKGQDPAFTEA---------------------LKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRN-GVRPWRSPEMDTQLRCRWLH-HSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKR----TNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHID-SQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPC 571
            D E Y+ +P+NA+ +V RL T++  L L++L+   +       L      FPT  +      ++  LQ+ Y L+   L+ G   + G  ++S   L   +   +G  A N G + + + W++ A K+ D+G+D   ++                      L R++ S +  H++        G + R F K  + K R+K   K ++       +++   ++  +       +   +CR  GV+   +P    +L CR+   + NP L +APFK E   + P + +++D M + E+E  +  AKP L R+     D K         R SK +WL E                 + P +                   +R++  TGL  +   +AE  QVANYG+GG Y+ H D S+  FD  +               G+RLATF+ Y+SDV+AGG TVFP  G +  P KG+A+FW NL  +G+ D  T H  CPVL+G KW++NKW          PC
Sbjct:   75 DPEGYLAHPVNAYKLVKRLNTDW--LELENLVLQDTTNGFITNLTIQRQFFPTEEDETGAAKALMRLQDTYKLDPETLSRG--NLPGTKYRSS--LTVSDCFGMGKTAYNDGDYYHTVLWMEQALKQHDEGEDTTVSKVEILDYLSYAVFQFGDLHRAMELTRRLISLDSTHERA-------GSNLRYFEKLLE-KEREKPSNKTVATT---EPVVQSGAYERPLDYLPERDIYEALCRGEGVK--MTPRRQKRLFCRYHDGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLARATV--RDPKTGVLTVASYRVSKSSWLEED----------------DDPVVA---------------KVNQRMQQITGLTVK---TAELLQVANYGMGGQYEPHFDFSRRPFDSTLKSE------------GNRLATFLNYMSDVEAGGATVFPDFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGNEFLRPC 528          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|129365|sp|P16924.1|P4HA1_CHICK (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1)

HSP 1 Score: 168.703 bits (426), Expect = 7.836e-44
Identity = 151/513 (29.43%), Postives = 229/513 (44.64%), Query Frame = 0

Query:   80 DIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANG-IIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKR-DKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSK--PFDPKLRKK----KKFKKISANPFQAELLKHTLFDLSVSQTTRVA-------LSHEMCRNGVRPWRSPEMDTQLRCRWLH-HSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKR--TNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLS 574
            D E ++G+P NAF ++ RL T + +L  +SL+           +      FP   +      ++  LQ+ Y L+   L+ G +  V+ KSF     L A +  E+G     +  + +   W++ A K+ D+G+  +  +       S  +Y      Q+G   K      +    DP+ ++     K F+ I A   +A        D +  +T             +EM   G     +P    +L CR+   + NP   L P K E   ++P + +F D + + E+E  +  AKP L R+     +  +  T   R SK AWL                   E P           VV R   NT  RI+  TGL+     +AE  QVANYG+GG Y+ H D         GR   P+ F+     G+R+AT++ Y+SDV AGG TVFP +G S  P KG+A+FW NL  +G+ D  T H  CPVL+G+KW++NKW+    Q    PC LS
Sbjct:   57 DPEGFLGHPANAFKLMKRLNTEWGEL--ESLVLKDMSDGFISNMTIQRQFFPNDEDQTGARKALLRLQDTYNLDTDTLSRGNLPGVKHKSF-----LTAEDCFELGKIRYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVY------QQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTDKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWL----------------SGYESP-----------VVSR--INT--RIQDLTGLDVS---TAEELQVANYGVGGQYEPHFD--------FGRKDEPDAFKE-LGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLS 513          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. SwissProt
Match: gi|2507090|sp|P13674.2|P4HA1_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor >gi|75061858|sp|Q5RAG8.1|P4HA1_PONAB RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor)

HSP 1 Score: 168.318 bits (425), Expect = 1.448e-43
Identity = 167/577 (28.94%), Postives = 261/577 (45.23%), Query Frame = 0

Query:   27 LFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSL--LTDSSLPDL--QRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANG-IIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKR-DKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPF--DPKLRKK----KKFKKISANPFQAELLKHTLFDLSVSQTT----RVALSH-------EMCRNGVRPWRSPEMDTQLRCRWLH-HSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRS-----VTGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLS 574
             F+SI ++  L   E      +  + +  E   +  +K+ +        +   D E +VG+P+NAF ++ RL T + +L    L  ++D  + +L  QR+       FP   +      ++  LQ+ Y L+   ++ G +  V+ KSF     L A +  E+G  A  +  + +   W++ A ++ D+G+     +       S  +Y      Q+G   K      K    DP+ ++     K F+ I A   + ++ K    D S  +TT     VA+ +       EM   G     +P    +L CR+   + NP   LAP K E   ++P + +FHD + + E+E  +  AKP L R+     +TG  +   T   R SK AWL                   E P           VV R       RI+  TGL+     +AE  QVANYG+GG Y+ H D          R   P+ F+     G+R+AT++ Y+SDV AGG TVFP +G S  P KG+A+FW NL ++G+ D  T H  CPVL+G+KW++NKW+    Q    PC LS
Sbjct:   21 FFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAE-KLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQY------FPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSF-----LTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVY------QQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAK--EKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLE---TVHYRISKSAWL----------------SGYENP-----------VVSRINM----RIQDLTGLDVS---TAEELQVANYGVGGQYEPHFD--------FARKDEPDAFKE-LGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLS 531          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1228404351|ref|XP_021961276.1| (prolyl 4-hydroxylase subunit alpha-2-like [Folsomia candida] >gi|1215259448|gb|OXA45773.1| Prolyl 4-hydroxylase subunit alpha-2 [Folsomia candida])

HSP 1 Score: 284.648 bits (727), Expect = 1.520e-83
Identity = 193/591 (32.66%), Postives = 303/591 (51.27%), Query Frame = 0

Query:    7 LLIVIVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPA---FTEALKRK-----IKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLR--LAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSV-TGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLET-QKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSPSQGNYPVFQR 585
            LL+  V    ++ V     ++FSSIAE+ERLY+ E    + +  +  +L R+    +KY++  + + P   +      V NP  AF ++ R   +++ +   ++   +   D+  ++       PT  +F     ++  LQ+ Y LN S++  G +       QS + L AR+L+ +G  A N+G +D  +SWL++A  R + +  +   F+    R+     +++ +  HD +LD+RG  G++ + F KP D KL K+KK+  +S   ++ +L  +T  + +       AL    CR G+R  RSP++D  LRCR++  + P  R  + PF++E    +P++   +DFM+ +EM+ F+  A   L RS+  G + Q     IRTSKQAWL +   +F                           VD      ++RI  ATGLE   +  +AE FQVANYG+ G Y  H D   Y  GE   + +P + R     G+R+ATFM YL +V AGG TVFP +G+  +P KG+A+FW NL+  G  D LT H GC VL GSKWITNKW+  N QFQ   C   P       F++
Sbjct:   11 LLVTFVVVSANYVVYAKESEIFSSIAELERLYRNEAAVVKQLDEYASVLARNLHVIKKYIE-DFNKIPSFDEETAAEVVANPFTAFHILKRTVVDWE-VVADTIKNHTKAADVLDEVMQKGDKMPTFDDFIGGSMALVGLQDTYGLNLSEVIYGTLGSESVP-QSPYELSARDLLFLGKAAFNKGLYDRSISWLEMALSRSEEESQSVSFFSPTATREEILPFLETTKRVHDDVLDKRGPTGENWQTFLKPVDQKLAKQKKY--LSVKKYKPKL--YTPLNGAEENENYKAL----CR-GIR-LRSPKLDAPLRCRYV--ARPLTRSFIGPFQIEEHAIEPYIVTINDFMNEDEMKSFRDTASSRLKRSLHQGRDGQYVATDIRTSKQAWLTQEDGSF---------------------------VD----TISRRISDATGLEIFPENLAAEIFQVANYGMAGRYTAHTD---YLTGE-DHIRNPLEIRR----GNRIATFMVYLENVGAGGSTVFPYIGVQVKPKKGTAVFWWNLKRDGSGDPLTRHAGCSVLYGSKWITNKWVRYNAQFQRALCIPEPEDNRSASFRQ 547          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|321458081|gb|EFX69155.1| (hypothetical protein DAPPUDRAFT_228756 [Daphnia pulex])

HSP 1 Score: 266.929 bits (681), Expect = 1.506e-76
Identity = 191/597 (31.99%), Postives = 295/597 (49.41%), Query Frame = 0

Query:    9 IVIVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYV-DLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGK-SFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQK--RDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRK--------------KKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYA-KPNLFRSVTGHEDQKR---TNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKP-FSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP-SQGNYP 581
            +VI   +GD A+     D++SS+AE+ERLY  E      + S  +L+++   A  +Y+ +LT      S ++D    + NP+NAF M+ RL   ++++   +L T  ++ D+ + +   S  FP   +      S+  LQ AY LN S LA+G +Q+  + SF S   L+AR+ + IG  A N+G +   + W+  A +  R +    A    +K  + +    HD +L+++G  G D R   +PF+ + +                ++ + I+  P  A     T      S+   + +   +CR G    R   +  +L+CR + H++P+  L P K+E  +  P++  FHDFM + E E F++ A    L RS  G +   +   T+  RTSKQ+W+ +                               VVD+     +KRI  + GL +Q     +E +QVANYG+GG Y  H D  G     +G  +  + FR     GDR+ TFM YL DV+AGG TVF   G+  +P KG A+FW NL+S    D+LT HGGCPVL GSKWI NKW  S +QF   PC   P S+ +YP
Sbjct:    8 LVIFLLIGDMALA---SDMYSSVAELERLYLKEQQAGLKLESLLDLIKKQTTAIDQYLSELTEIR---SANLDSRGVISNPINAFHMMRRLTIGWREVK-TTLETHGNVTDIVQDVFKISKSFPNDEDLSGAAFSLAQLQGAYRLNVSQLASGNVQLSDRISFPSVRGLNARDCLFIGKHAFNKGLYGQAVDWISTAAEVARSENNRSASVSEIKPFLSTAIRVHDDVLEKKGPIGADWRTNPEPFERRAKSLRRARRSQEQQQQQTQRMRDITYTPLGAGHGLATNKSPEWSEWEELEVFFRLCREGEEKSRPTGLKGRLKCRQISHTHPYFILRPLKLEEHSLVPYIAVFHDFMSDAETEIFKSLAMAERLERSAHGSKRPGQGGVTSDKRTSKQSWVEDGSH---------------------------HVVDQ----ISKRISDSVGLNSQPSNVGSEHYQVANYGIGGRYTPHTD-HGVLSKSMGGPSEFDLFR-----GDRILTFMTYLDDVEAGGATVFTHAGVVVRPKKGMAVFWWNLKSDSNGDTLTRHGGCPVLHGSKWICNKWFHSGEQFLKRPCAKKPRSRISYP 560          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1022769696|gb|KZS14171.1| (Uncharacterized protein APZ42_020808 [Daphnia magna])

HSP 1 Score: 262.307 bits (669), Expect = 4.896e-75
Identity = 179/566 (31.63%), Postives = 282/566 (49.82%), Query Frame = 0

Query:   26 DLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGK-SFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQK--RDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLR--------KKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRN-GVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKR---TNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKP-FSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            D++SS+AE+ERLY  E    + +  F  L+++  +A   Y  LT      S +++  + + NP+NAF M+ RL  ++Q +   +L T  ++ ++   + N S  FP   +      S+  LQ AY LN S LA+G +Q+  + SF S   L+AR+ + IG  A N+G++D  + W+  A +  + +    A    +K  + +    HD +L+++G  G++ R   +PF  +++        K+++      N  +    K  +++          +   +CR    RP R   + ++L+CR + H+ P+  + P K+E  +  P++  FHDFM + E E F++ A  NL RS  G +   R   T+  RTSKQ+W+++                               V+D+      KRI  + GL +Q     +E +QVANYG+GG Y  H D    F    G+ T  E  R     GDR+ TFM YL DV+ GG TVF   G++ +P KG ++FW NL S    D+ T HGGCPVL GSKWI NKW  S +QF   PC   P
Sbjct:   22 DMYSSVAELERLYVKEQQVGQRLEHFLLLIKQQTEAIDHY--LTELREIRSANLESRAVISNPINAFHMMRRLTISWQTVK-TALETQVNVTEIVEDVLNISKSFPNDEDLSGAAFSLAQLQGAYRLNVSQLASGNVQMSDRISFASLRGLNARDCLFIGKHAFNKGFYDQAVDWISTASEVAQSESNQSATVAEIKPFLSTAIRVHDDVLEKKGPLGENWRTNPQPFKRRVKTVSGGNQSKQQRIADAKNNGRRLATDKSPVWE-------ETEIFFRLCRGEQSRPTR---LTSRLKCRHISHTQPYFIVRPLKLEEHSLVPYIAVFHDFMSDAETELFKSLAIENLERSAHGSKRSARQGVTSDKRTSKQSWVQDGSH---------------------------HVIDK----ITKRISDSVGLNSQPSNVGSEHYQVANYGIGGRYTPHCDHGVLFQSR-GQPTEFELMR-----GDRILTFMTYLDDVEEGGATVFTQAGVAVKPKKGMSVFWWNLMSDLSGDTSTRHGGCPVLHGSKWICNKWFHSGEQFLKRPCGNKP 537          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|321461762|gb|EFX72791.1| (hypothetical protein DAPPUDRAFT_308081 [Daphnia pulex])

HSP 1 Score: 249.98 bits (637), Expect = 2.102e-70
Identity = 181/571 (31.70%), Postives = 276/571 (48.34%), Query Frame = 0

Query:   21 KGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLN-LKSLLTDSSLPDLQRKLA-NDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLA----NGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIA--QKRDKGQD-----PAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRK--KKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSV-TGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            K + DD+FSS++++E L   E      + SF    ++     + YV  TY +  MSGD+     V NP++A+ ++ RL  ++  +  +    ++SS   L   L   ++ +FP   +      ++  LQ+ Y LN +DLA    NG+   +G+ FQS   L  R+ + +   A   G++D  L W + A  Q   KG +       F   +K  ++     HD +L+++G  G + +  S P D + R    +K+  ++   F  +L +H   D       R      +CR      RS  ++  LRCR +   +P L L P K+E  +  P +   HD +   + E  +   +P L  S+  G E +   + IRTSK AWL+E              ++  +P I            RH      R+++ATGL      ++E FQ+ANYG+GG Y+ H D+  + D     V   +Q   N  VGDR+AT M YLSDV+AGG TVFP  G++  P KGSA FW NL  +G+ D  T HG CPVL GSKW++NKWI   DQ     C L P
Sbjct:   28 KPETDDIFSSVSKMEALVSQENAIVNMLDSFLIDAKQRISIIQNYV-TTYRQ-VMSGDLSSSKLVANPVDAYHLLKRLTIDWDVVERVLEAESNSSRNALNHVLVLRETSVFPNSEDLHGAAIALVRLQDTYQLNVTDLADGHYNGVDHSKGQVFQSRRDLSGRDCLFMAQHAFADGYYDTALQWAEAALGQFARKGDNYGSELETFRNEVKHFVEFASRVHDDVLERQGPRGVNWQTNSHPVDRQFRSGTSEKYDSLAEQQFTPKLYQHQSEDEENEHYER------LCRG--EKLRSANIEAGLRCRLVTRGHPALLLQPIKVEEQSLDPMIVVLHDLITERQTEILRQLGEPKLATSLHRGGEGKFVRSMIRTSKNAWLQEH-------------ENASLPAI------------RH------RMELATGLIYGPETASEYFQIANYGIGGLYKTHTDNVIHPD-----VRPEDQDPWNLYVGDRIATLMVYLSDVEAGGATVFPRAGVTCWPRKGSAAFWWNLYKSGEPDLTTRHGACPVLHGSKWVSNKWIRQYDQMFVAKCGLHP 552          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1215266811|gb|OXA53125.1| (Prolyl 4-hydroxylase subunit alpha-2 [Folsomia candida])

HSP 1 Score: 229.565 bits (584), Expect = 1.746e-62
Identity = 169/602 (28.07%), Postives = 271/602 (45.02%), Query Frame = 0

Query:    1 MRTIRVLLI---------VIVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLAN--DSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSF-QSDHI-------LDARELVEIGIGACNQGWWDNGLSWLK----IAQKRDKGQDPAFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKI---RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDS-QGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSP 575
            +R + ++LI         ++ G      ++   +D+FSS +++E L   E      + +FTE     A   + YVD       +      +  V +P++A+ +  RL  +++D+ L      +    +   + +  +   FP   +      ++  LQ+ Y LN + L  G++    KS   SD I       L AR+L+ +G  A + G +   + WL+    +A K   G        +   ++S    HD +L+++G  G   +    P +P L +  K+     N FQ +L +    +       R      +CR      R   M+ QL+CR  +  +P+L ++P K+E  +  P++   HDF+   + +      KP L+RS   H  +   + +   R SK AW                +KD E               D+      +R+++ +GL+  +P  AE +Q+ANYGLGG Y  H D      D  I   T  E+F     VGDR+AT M YL++V AGG TVFP  G++ +P KGSA FW NL  +   D  T HG C VL G KW++NKWI SN+QF   PC L P
Sbjct:   36 IRLVSIVLIALFQSHFVTIVSGVRESNELRNAREDVFSSTSKLEELVHNEFQVLDLLTTFTEYTIEKATMIKGYVD--NQRRLLGNRKSAKDIVAHPVDAYVLTRRLTADWEDVQLALHAVSNYSTGVYSWIGSFREGSNFPQGDDLNGAAQALVRLQDTYRLNMASLTQGVVNPLQKSVVNSDSIKFEGVEGLTARDLLFLGKHAYSNGLYHRAIEWLQGAESVAAKESNGT--MLLSEIAPSLQSAIDTHDDILERQGPQGTGWKTNPVPINPILSRSAKYNLTVPNTFQTKLFQRQTEEEETEHYVR------LCRG--EKLRPAMMEAQLKCRMKNDGSPYLIVSPLKIEEESHDPYIVIVHDFITEKQADALVELGKPKLYRSRHRHHKEGNLDVVSQKRISKNAW----------------IKDWE---------------DKLMIKITRRVELVSGLKAFQPKEAEDYQIANYGLGGLYVPHTDHLMNNPDKSI--YTQWERF-----VGDRIATLMIYLTNVGAGGGTVFPRAGVTLRPRKGSAAFWWNLDKSLVGDENTRHGACAVLYGDKWVSNKWIRSNEQFLKAPCGLHP 587          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|952514029|gb|KRT80516.1| (hypothetical protein AMK59_8778 [Oryctes borbonicus])

HSP 1 Score: 226.868 bits (577), Expect = 8.042e-62
Identity = 178/606 (29.37%), Postives = 279/606 (46.04%), Query Frame = 0

Query:    2 RTIRVLLIVIVGSLGDWAVKGDNDDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLA--NDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDPAFTEALKRKIKSGEIYH-------------------DKLLD----QRGQFGKDHRCFSKPFDPKLRKKKKFKKISANPFQA--ELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPE-MDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKI-----RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLS 574
            + + +LLI ++ S  ++A      ++F+++A++E L Q E      + S+    ER     RKY    Y E       DI+SYV NP+NA+ +V +L T+++ +    L+T +   D+   L+   D L FP+  +      ++  LQ+ Y L+ + LANG IQ  G  + S+  L A +  E+G  + N G   +   W+  A  R K +       + R +   +I                     +KLL+         G       +    + RK+K   ++   P +   E+   T+   +  +    AL   +CR        P+ +  QL+CR++ + NPFL++APFK+E    QP +  FHD M++ E+   +  A+P   R+        RT K+     R SK AWL+E                                  RH  N A+R++  TGL  +   +AE  QV NYG+GGHY+ H D          R      F+ +   G+R+AT + Y+SDV  GG TVFP L ++  P KG+A FW NL S+G+ D  T H  CPVL GSKW++NKWI  ++Q    PC L+
Sbjct:   13 KKLGILLITLLCS--NYAFS----EVFTALADLEELLQTENFLISAMNSYITSQERKLWQLRKYFQ-EYTEQYQKASEDIQSYVANPVNAYLLVKKLTTDWKQVT--ELITTNVGEDVISNLSEYKDVLTFPSDEDLNGAAIALMRLQDTYRLDTASLANGEIQ--GVKYASE--LTAEDCFELGRQSYNVGDHYHTQLWMTEADDRLKRE-------VNRTVDRSDILEYLAFSTFKLGNTVLALDLTNKLLELIPSHSRALGNKIYYEDEIAKTERRKRKGEDEVPEAPPEEYDEVSSMTMSPSTAPEKEARALYEALCRGDTT---IPDTISIQLKCRYVDNGNPFLKIAPFKIEEAYTQPRIWIFHDVMYDTEISTIKQLAQPRFQRATV---QNSRTGKLETAHYRISKSAWLKER-------------------------------EHRHIANVAQRVEDMTGLNMK---TAEELQVVNYGIGGHYEPHFD--------FARKEEKNAFK-DLGTGNRIATVLFYMSDVAQGGATVFPYLNVALWPKKGTAAFWFNLHSSGEGDKTTRHAACPVLAGSKWVSNKWIHEHEQEFRRPCGLT 549          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1022775144|gb|KZS19060.1| (Uncharacterized protein APZ42_014553 [Daphnia magna])

HSP 1 Score: 224.172 bits (570), Expect = 8.174e-61
Identity = 168/568 (29.58%), Postives = 266/568 (46.83%), Query Frame = 0

Query:   25 DDLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQR----KLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANG----IIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQK---RDKGQDPAFTEALKRKIKS----GEIYHDKLLDQRGQFGKDHRCFSKPFDPKLRKK---KKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSV-TGHEDQKRTNKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPL 573
            DD+FSS++++E L   E      + SF    ++       YV  TY +  + G+++    V NP++A+ ++ R   +++    + +L D S+          +  D  +F    +      ++  LQ+ Y LN + LA+G    + Q + + FQS   L +R+   I   A   G++D  L W + A +   R++      TE L  +IK       I HD +L+++G  G   +  S P + KL +    +K++ +S   F  +L +    D       R      +CR      R+ ++D  LRC  +   +P L L P K+E  +  P +   HD + +++ +  +  A+P L  S   G +     + IRTSK AWL E+             ++  +P I                   +R+++ TGL      ++E FQVANYG+GG Y+ H DS       + R  +     H   +GDR+AT M YL+DV+AGG TVFP  G++  P KGSA FW NL   G+ D  T HG CPVL GSKW++NKWI   DQ     C L
Sbjct:   29 DDVFSSVSKMEGLVHQEKKLVDTVDSFLIEAKQRLAIVEDYV-TTYRQA-IKGELNSAWLVANPVDAYHLIKRFTVDWR--TTEQILRDVSITSNDACNHVMMLRDKSVFAESHDLHGAAIALIRLQDTYKLNVTALADGRYTGVDQSKEQVFQSLQDLSSRDCFFIARHAFADGYYDTALEWGEAALRQLAREEDNVGPTTEILGDEIKHFMNYASIVHDDVLEKKGPLGMHWQTNSNPVNKKLFRSGSSEKYESLSEQKFTPKLYQQQSEDEQNESYER------LCRG--EKLRTVKIDASLRCHLVTRGHPALLLQPAKVEQQSLDPMIVVLHDLLTDHQTDILRQLAEPKLATSSHLGPDGTFIRSMIRTSKNAWLEEN-------------ENASLPAI------------------RRRMEIVTGLIYTPETASEHFQVANYGIGGLYKTHTDST------VNREDTDPWMLH---IGDRIATLMVYLTDVEAGGATVFPNAGVTCWPRKGSAAFWWNLYKNGEPDLTTIHGACPVLHGSKWVSNKWIRQYDQMFVAKCGL 544          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1133428132|ref|XP_019869955.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1 [Aethina tumida])

HSP 1 Score: 223.402 bits (568), Expect = 1.088e-60
Identity = 169/567 (29.81%), Postives = 269/567 (47.44%), Query Frame = 0

Query:   26 DLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRK--LANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKRDKGQDP------------AFTEALKRKIKSGEIYHDKLLDQRGQFGKDHRCFSKP--FDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKI---RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPL 573
            ++F+++A++E L   E      + ++ +  E+     ++YV L Y E     + D++ Y+ NP+NA+ +V RL T++Q   ++SL+T     D      L  +++ FPT  +      ++  LQ+ Y L+ S LA G  ++ G  + ++  L A +  E+G  + N G + +   W++ A  R K +              AF+   +  I       +KLL+         R       ++ +L+K  ++K+   +  ++E +      + V  T       ++CR  V     PE   +L+CR++HH+ PFLR+APFK+E     P +  FHD M +NE+E  +  A P  FR  T    Q    +I   R SK AWL+E                        +  KY+        + A+R++  TGL  +   +AE  QV NYG+GGHY+ H D          R      F+ +   G+R+AT + Y+SDV  GG TVFP +G +  P KG+A FW NL  +G  D LT H  CPVL GSKW++NKWI    Q    PC L
Sbjct:   19 EVFTALADLEDLLHTEGLLINTLQNYIQAEEQKVDLLKRYVKL-YQEQHNRAEEDVQLYISNPINAYTLVKRLTTDWQ--QVESLMTTDISKDFLNNITLYKETMKFPTDEDLNGAAVALTRLQDTYRLDTSSLARG--ELNGVKYSAE--LSAADCFELGRQSYNNGDYYHTQLWMREADARLKREANVTVDKSDILEYLAFSTYKQGNIHLALDLTNKLLEI---LPSHSRALGNKVYYEEELQKSSEYKRKGDD--ESEAVPAVEVPI-VPDTYSREHYEQLCRGEVS--LPPETVAKLKCRYVHHNKPFLRIAPFKVEEAFLGPNLYIFHDVMSDNEIETVKRLAHPR-FRRATVQNSQTGELEIAQYRISKSAWLKE------------------------HEHKYIA-------DIAQRVEDMTGLTME---TAEELQVVNYGIGGHYEPHFD--------FARKDEKNAFK-SLGTGNRIATVLFYMSDVAQGGATVFPQIGAALWPKKGTAAFWYNLFPSGDGDRLTRHAACPVLAGSKWVSNKWIHERGQEFLRPCTL 526          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1022772651|gb|KZS16808.1| (Uncharacterized protein APZ42_017385 [Daphnia magna])

HSP 1 Score: 219.55 bits (558), Expect = 2.084e-59
Identity = 171/582 (29.38%), Postives = 271/582 (46.56%), Query Frame = 0

Query:   26 DLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDID-----IESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTD---SSLPDLQRKLANDSLLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLK-------IAQKRDKGQDPAFTEALKRKI-KSGEIYHDKLLDQRGQFGKDHRCFSKPFDPK--------LRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALSHEMCRNGVRPWRSPEMDTQLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRS-VTGHEDQKRT--NKIRTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPF-SAEAFQVANYGLGGHYQLHID--SQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPLSPSQ 577
            D+ S+ A++  ++Q+E   +  +       E   +  R+Y+   Y +  ++  +D     +E   GNP++A+ ++ RL  ++Q L  K + TD   S+L  L+    N+   FP   +F    S++  LQ+ Y LN ++L  G   +RG+   ++  L A + + +G  A ++G +   + WL+       +   +   QD    E +++ I K  E  H+    QR     D + F  P   K        + + +K+ K  A  F A                       +CR       S E+  QL+C +  H  P+L L P K+E  + +P +  FHD +++ E+E  +  A P L R+ V   +   +T  + +RTSK AWL +                        +PM +           ++RI + TGL+    F  AE  QVANYG+GGHY  H D   +G  D E+G++ + E        GDR+ATFM YL+DV+ GG T FP  G++ +P KG A FW NL+  G+ D  T HG CPVLLG KW++NKWI    Q    PC L P +
Sbjct:   22 DMHSASADLASIFQMEQQVNNVLSELVSQTETKLEIIRQYLK-EYEQSVINETMDDKNEFMERLAGNPIHAYRLMKRLYFDWQ-LIEKEIKTDNWQSTLIKLEEVRNNEK--FPKEEDFLGATSALVRLQDTYELNITELVRG--NLRGRQTHAE--LTALDCLFMGKHAFDEGAYARAVEWLEESYILAGLENNQTIRQD-HIMEVIQQAIDKHDEALHEA--QQRMAVLSDTKSFRLPLKEKPAVLQRNEMIRSRKYDKDDAAKFNA-----------------------LCRG--ESLLSEEILGQLKCWYETHKEPYLFLMPIKIEQNSIRPAIYTFHDVLNDLEIETIKELATPLLARAKVLAKQAGSKTTVSNVRTSKTAWLEDE----------------------LHPMLH---------RISQRIALVTGLKVNSSFDEAEILQVANYGIGGHYSPHHDYLMKGKTDEELGKMNARE-----LATGDRIATFMFYLNDVERGGSTAFPRAGVAVKPVKGGAAFWYNLKRNGQGDPDTLHGACPVLLGHKWVSNKWIRETAQSFNRPCLLDPDE 531          

BLAST of af495541_1prolyl 4-hydroxylase alpha-related protein ph4 vs. nr
Match: gi|1344766136|ref|XP_023936563.1| (prolyl 4-hydroxylase subunit alpha-1 isoform X3 [Bicyclus anynana])

HSP 1 Score: 217.238 bits (552), Expect = 2.603e-58
Identity = 172/577 (29.81%), Postives = 278/577 (48.18%), Query Frame = 0

Query:   26 DLFSSIAEVERLYQIEVGFSRDILSFTELLERSAQAFRKYVDLTYPEGPMSGDIDIESYVGNPLNAFGMVGRLGTNFQDLNLKSLLTDSSLPDLQRKLANDS-LLFPTLSNFEEVVSSIGLLQEAYALNCSDLANGIIQVRGKSFQSDHILDARELVEIGIGACNQGWWDNGLSWLKIAQKR--DKGQDPAFTEA-----------LKRKIKSGEIYHDKLLDQRGQFGK---DHRCFSKPFDPKLRKKKKFKKISANPFQAELLKHTLFDLSVSQTTRVALS-HEMCRNGVRPWRSPEMDT------QLRCRWLHHSNPFLRLAPFKMELVNEQPFVCQFHDFMHNNEMEHFQAYAKPNLFRSVTGHEDQKRTNKI-----RTSKQAWLRESWFTFNVSQTAESVKDMEIPPIPFNPMKYVQVVDRHGFNTAKRIKMATGLETQKPFSAEAFQVANYGLGGHYQLHIDSQGYFDGEIGRVTSPEQFRHNTVVGDRLATFMAYLSDVQAGGHTVFPLLGISAQPTKGSAIFWINLQSTGKRDSLTTHGGCPVLLGSKWITNKWISSNDQFQTLPCPL 573
            +LF++I++VE L +       D+  +    E+     ++++     E   + D DI +Y+GNP+NAF ++ RL T+  DL  K++++ ++       + N S + +PTL +      ++  LQE Y L  +DL+ G  ++ G  + +   + A +  ++G    N+  + N L+W+  A ++  +  ++  FTE            L   +K   ++  KLLD   +  +   +   + K  + K  K K+  ++  +  + +L ++ L D    +    ALS +E  R         EM+       +L CR+L+ +NPFL LAP KME +   P +  FH+ + ++E+EH +  AKP  FR  T H+   +T ++     R SK  WL                KD + P I                  ++R+   TGL      SAE  QV NYG+GGHY+ H      FD    +  + E+F      G+R+AT + Y+SDV  GG TVF  LG+S  P KG+A+FW+NL  +G+ D  T H  CPVL GSKW+ NKWI    Q   LPC L
Sbjct:   25 ELFTAISDVEPLLETHRKIIDDLDDYINKEEKRLSILKRHLVKYKNEHEKAMD-DIPNYLGNPINAFTLIKRLTTDL-DLIEKNIISGTAYIKNITIMNNHSDVKYPTLEDLVGAAQALTRLQETYHLEVNDLSEG--RLNGVIYSTS--MSAGDCYQLGKALYNEKDYTNALAWMMQALRKYSEDEEEKNFTEVDILEYISFSHYLLDDVKKAMLWTKKLLDLDPEHSRALGNLPHYKKALEEKEAKNKR--RLRGDTGEVDLDEY-LTDADQPEIKPEALSKYEKERQSYEALCRGEMEMPSKITKKLYCRYLNENNPFLILAPVKMEYMYLNPDIVVFHEVLSDSEIEHIKELAKPR-FRRATVHD--PKTGELVPAHYRISKSGWL----------------KDEDSPIIA---------------RVSRRVADFTGLSM---MSAEELQVVNYGIGGHYEPH------FDFARKQEHAFEKFN-----GNRIATVLFYMSDVAQGGATVFTELGLSVFPVKGAAVFWLNLHPSGEGDLATRHAACPVLRGSKWVCNKWIHQGGQELILPCDL 544