BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000006113 (supercontig:LSalAtl2s:LSalAtl2s332:452120:457604:1 gene:EMLSAG00000006113 transcript:EMLSAT00000006113 description:"augustus_masked-LSalAtl2s332-processed-gene-4.0")

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 286/609 (46.96%), Postives = 396/609 (65.02%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE-------------STLKHAHRLGVDP--EVSSDSALRKESPLSA----RMQSMHQHYPNLVDCYFETR-RSVASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRLGNKP 629
            ++ CP+CF++ KE  +T+CGHSFC  CL  +L+    +CPKC   ++   ++ PNF LN +  ++   QK                     + TP                 +  +NLS  D+  L G L + K+ + +    ++  LL EFL+ LK EK   L + +++ +++ +DL+             S         V P  EVS+ +   KE P  +    R + MH+H  +L   Y+ +R + ++  P   S   +  GL  FS  L+KFTKY+ ++ +A L+Y +DM++N SIVSSI+FDKD EFFAI GVTKRIK+YDY+ V+ D+VDIHYP  EM+S SKISC+SW+SFHK  L+SSDY+G+V +WDA +  K +++ EHEKRCWSVDFN +DTKL AS SDDARVKLWST+++HS+ASLEAKANVC VKFNP SC+H+AFGSADHCVHY+DLRN K+ L +F GHKKAVSYV+FLN+  ++SASTDSQLKLW+V +  C++S+ GH NEKNFVGL +D +Y   GSENN   ++YKGL K LFSFKFD+ +T   RN++  D +NEFVSA+CWR+GT+ ++ ANSQG +K+  L + P
Sbjct:    9 DFSCPVCFDILKEPHMTRCGHSFCYSCLNKSLK-IKPQCPKCSFDLHPDKDIYPNFTLNQIISKL---QKD--------------------NDTPQ----------------IPTSNLSMSDINRLLGTLHRRKKELEKESFLTQNELLAEFLSHLKVEKDAELLRIRRESEVIQADLDHVNQVLQDYRTNSSNFASGDENNVVPSLEVSNPTINIKEPPTESSFPMRRRRMHKHVLDLSSAYYSSRAKEMSFTPEECSSSLDYSGLKDFSLCLNKFTKYNTVKPLATLSYMTDMFNNTSIVSSIDFDKDNEFFAIGGVTKRIKIYDYSVVLNDLVDIHYPSMEMVSSSKISCISWSSFHKGTLVSSDYEGSVVVWDANSNSKIRTFQEHEKRCWSVDFNRIDTKLVASGSDDARVKLWSTNIHHSVASLEAKANVCCVKFNPESCYHLAFGSADHCVHYYDLRNTKNHLNLFKGHKKAVSYVKFLNSESIVSASTDSQLKLWNVNRSDCMRSFTGHTNEKNFVGLATDGEYITCGSENNDLHVYYKGLPKSLFSFKFDSVKTFLDRNNRE-DNANEFVSAVCWRQGTNVVLCANSQGVIKVLELTSGP 576          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000002459 (supercontig:LSalAtl2s:LSalAtl2s1472:24513:26207:-1 gene:EMLSAG00000002459 transcript:EMLSAT00000002459 description:"maker-LSalAtl2s1472-snap-gene-0.16")

HSP 1 Score: 81.2629 bits (199), Expect = 4.843e-16
Identity = 58/167 (34.73%), Postives = 81/167 (48.50%), Query Frame = 0

Query:  374 VSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVE-------HEKRCWSVDFNSVDTKLFASASDDARVKLWST-SVNHSIASLEAKANVCSVK---FNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRF--LNATELLSASTDSQLKLWDVEK 527
            +SWN      LLS+  D T+ LWD     K    ++       H      V ++ +   LF S +DD ++ +W T S  H+  S    A+   V    FNP S F +A GSAD  V  +DLRNLK  L  F  HK  +  V++   N T L S+ TD +L +WD+ K
Sbjct:  279 LSWNPNLNGYLLSASDDHTICLWDINAQPKDNKVIDAKTIFTGHTAVVEDVAWHLLHEXLFGSVADDQKLMIWDTRSATHNKPSHTVDAHAAEVNCLSFNPYSEFILASGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLHVWDLSK 445          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000001111 (supercontig:LSalAtl2s:LSalAtl2s1186:117586:119588:-1 gene:EMLSAG00000001111 transcript:EMLSAT00000001111 description:"maker-LSalAtl2s1186-snap-gene-1.40")

HSP 1 Score: 76.6406 bits (187), Expect = 9.207e-15
Identity = 68/252 (26.98%), Postives = 118/252 (46.83%), Query Frame = 0

Query:  322 VSSIEFDKDAEFFAIAGVTKRIKVY-DYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL-NATELLSASTDSQLKLWDVEKKTCIQSYQGHINEK-----NFVGLTSDEDYFAGGSENNSFFLF 565
            VS+++F  + E+ A +   K IKV+  Y+      +  H           IS V+W+S     L+S+  D T+ +W+  + +  K+   H    +  +FN   + L  S S D  V++W       + +L A ++ V +V FN      V+  S D     +D  + +   T+       VS+V+F  N   +L+A+ D+ LKLW+  K  C+++Y GH NEK     NF    +   +   GSE+N  +++
Sbjct:   33 VSAVKFSPNGEWLASSSADKLIKVWGSYDGKFEKTISGH--------KLGISDVAWSS-DSRLLVSASDDKTLKIWELSSGKCLKTLKGHSNYVFCCNFNP-QSNLVVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVS-SSYDGXCRIWDTASGQCLKTLIDNDNPPVSFVKFSPNGKYILAATLDNTLKLWEYSKGKCLKTYSGHRNEKYCVFANFS--VTGGKWIVSGSEDNMVYIW 271          

HSP 2 Score: 59.6918 bits (143), Expect = 2.231e-9
Identity = 47/176 (26.70%), Postives = 84/176 (47.73%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN--VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLN-----ATELLSASTDSQLKLWDVEKKTCIQSYQGHIN 539
            + C ++N    N ++S  +D +V +WD RT +  K+   H     +V FN  D  L  S+S D   ++W T+    + +L    N  V  VKF+P   + +A  + D+ +  ++    K  L  + GH+    Y  F N        ++S S D+ + +W+++ K  +Q  QGH +
Sbjct:  117 VFCCNFNP-QSNLVVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNR-DGSLIVSSSYDGXCRIWDTASGQCLKTLIDNDNPPVSFVKFSPNGKYILA-ATLDNTLKLWEYSKGKC-LKTYSGHRNE-KYCVFANFSVTGGKWIVSGSEDNMVYIWNLQTKEIVQKLQGHTD 287          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000006156 (supercontig:LSalAtl2s:LSalAtl2s336:420428:421924:-1 gene:EMLSAG00000006156 transcript:EMLSAT00000006156 description:"augustus_masked-LSalAtl2s336-processed-gene-4.9")

HSP 1 Score: 66.2402 bits (160), Expect = 2.304e-11
Identity = 57/206 (27.67%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  374 VSWNSFHKNQ--LLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFN--------SVDTKLFASASDDARVKLWSTSVNHSIASLEAK-ANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFH--GHKKAVSYVRFLNATEL-LSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            +SW +F  N   LL+  + G   LW     ++ ++   H     S+ FN        S      AS S D  VKLW+   +  IA +E   + V    ++P+  F +A    D+    +DL  L+    V H  GH K V  + F N   L L+   DS  ++WD+    CI   +GH+     V  + D  + A GS +N+  ++
Sbjct:  207 ISWCTFSPNSQYLLTGSWSGLCKLWSVPDCKEIRTLRGHSSNIGSIVFNPHATLEGHSNKDPCMASCSQDGSVKLWNLESDEPIADIEGHDSRVSRCAYHPSGRF-LATCVYDNSWRLWDLEQLQE---VLHQEGHSKPVHCIAFQNDGSLALTGGLDSFGRIWDLRTGQCIMFLEGHLKGIIGVDWSRDGYHCATGSSDNACKIW 408          

HSP 2 Score: 54.6842 bits (130), Expect = 9.171e-8
Identity = 50/186 (26.88%), Postives = 78/186 (41.94%), Query Frame = 0

Query:  389 YDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAK-ANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPL 573
            YD +  LWD    Q+      H K    + F + D  L  +   D+  ++W       I  LE     +  V ++    +H A GS+D+    +DLR  K   T+   H   VS V F +   LL+AS D   KLW  +    + S +GH        ++ D  Y A  S + +F L+   + + L
Sbjct:  317 YDNSWRLWDLEQLQEVLHQEGHSKPVHCIAFQN-DGSLALTGGLDSFGRIWDLRTGQCIMFLEGHLKGIIGVDWSRDG-YHCATGSSDNACKIWDLRKRKIEYTI-PAHTNVVSNVVF-DEDYLLTASYDGTAKLWARKTWQPLASLKGHDLRLMDCAISPDRSYIATCSYDRTFKLWSXDIKQRL 498          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000009105 (supercontig:LSalAtl2s:LSalAtl2s573:267932:269786:1 gene:EMLSAG00000009105 transcript:EMLSAT00000009105 description:"maker-LSalAtl2s573-augustus-gene-2.14")

HSP 1 Score: 65.0846 bits (157), Expect = 2.960e-11
Identity = 56/203 (27.59%), Postives = 97/203 (47.78%), Query Frame = 0

Query:  374 VSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL---------NAT-ELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            V WN    N +LS+  D T  +WD++T    + +  H      VD+ + ++  FAS S D  + +    V   I S     N V S+K++P   F +A GS D  +  + + + +S +     H KA+  +++          NA   L+SAS DS ++LWDVE+ +C+ +   H    + V  + D  + A GS +   +++
Sbjct:  121 VKWNK-SGNYILSASEDRTTIIWDSQTGNHNQQFAFHSAPILDVDWQTDES--FASCSKDKCIHVCKLGVEKPIKSFYGHTNEVHSIKWDPQGKF-LASGSNDTTLKVWCM-DKESHVHDLKAHSKAIYTIKWSCTGPGTNNPNADLVLVSASYDSTVRLWDVERGSCLHTLTRHQETVSSVAFSPDGKFLASGSYDKFVYIW 318          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000005738 (supercontig:LSalAtl2s:LSalAtl2s309:736085:739351:-1 gene:EMLSAG00000005738 transcript:EMLSAT00000005738 description:"maker-LSalAtl2s309-augustus-gene-7.97")

HSP 1 Score: 65.855 bits (159), Expect = 4.070e-11
Identity = 59/238 (24.79%), Postives = 111/238 (46.64%), Query Frame = 0

Query:  303 IRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLS-SDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSV-NHSIASL-EAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSA----STDSQLKLWDVEKKTCIQS 533
            ++V+   N ++D Y     +  +++  D     I   + R+ +++ ND   D +  H P       S+ +CV+W    +N+ L+ +DY G +TLWDA   +  +    H  R  ++ ++  +    AS S    V +    + NH I +L E    VC + +N      +A G  D+ V  +D+R+L  P  V   H+ AV  +++   + +  A    ++D  LK+W+     C++S
Sbjct:  534 VKVLDAPNLSNDFY-----IRVMDWSSDNILSVI--FSSRVLLWNANDTSVDNLTEH-PEG-----SEYTCVAW----ENKYLAIADYSGHITLWDASVKKCLRRLSGHFNRVGTLGWSEHN---LASGSRSGEVLIHDVRIKNHIICTLNEHTQEVCGIDWNSGGTL-LATGGNDNTVFIWDIRSLDKPTKVLTQHQXAVKGIKWCPWSNITLATGGGTSDRCLKIWNASNGNCVKS 750          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000008384 (supercontig:LSalAtl2s:LSalAtl2s512:319189:321924:1 gene:EMLSAG00000008384 transcript:EMLSAT00000008384 description:"maker-LSalAtl2s512-augustus-gene-3.19")

HSP 1 Score: 63.1586 bits (152), Expect = 1.033e-10
Identity = 54/199 (27.14%), Postives = 95/199 (47.74%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLE--AKANVCSVKFNPT-SCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL-NATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            +  V +NS     L +SD D ++ LW     +  KS+  H     S  + S + K  AS SDD  +KL+  +    I S     +     + F+P+ SC  +A G++D  V  +D+R +K   + +  H   V+ V F  +   LLS S+D  +K++D+ +   +    GH  E   +  +   +YF+ G  +N+ F++
Sbjct:   33 VRSVDFNSDGSKXLSASD-DKSIKLWTVEKQRFIKSFNGHNNWVRSAKW-SXBQKXIASVSDDRSLKLFDENTGKEIHSFNEPGRGFGQCLSFHPSGSC--IAIGTSDKKVKMYDIRTMKLHQS-YTSHTGPVNQVAFHPSGCYLLSCSSDGFVKMFDIIEAQPVYGLTGHKEEVTAIEFSPKGEYFSSGGSDNAVFVW 226          

HSP 2 Score: 52.7582 bits (125), Expect = 2.407e-7
Identity = 32/95 (33.68%), Postives = 51/95 (53.68%), Query Frame = 0

Query:  483 LRNLKSPLTVFHGHKKAVSYVRF-LNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSF 576
            ++++K   T F GH+  V  V F  + ++ LSAS D  +KLW VEK+  I+S+ GH N       + ++   A  S++ S  LF +   K + SF
Sbjct:   16 VKSVKGQXTDFKGHQGPVRSVDFNSDGSKXLSASDDKSIKLWTVEKQRFIKSFNGHNNWVRSAKWSXBQKXIASVSDDRSLKLFDENTGKEIHSF 110          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000003862 (supercontig:LSalAtl2s:LSalAtl2s209:625539:633615:-1 gene:EMLSAG00000003862 transcript:EMLSAT00000003862 description:"maker-LSalAtl2s209-snap-gene-5.15")

HSP 1 Score: 62.7734 bits (151), Expect = 3.095e-10
Identity = 54/181 (29.83%), Postives = 81/181 (44.75%), Query Frame = 0

Query:  381 KNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAK-ANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRF-LNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSEN 559
            K+ LLS   DGT+ LW          Y  H    W V F S     FASA  D  V LWST  +HS+       ++V  + F+P +C ++A GS+D  V  +D  +  + + +  GHK AVS + F  +   L S   DS++  WD+     +     H      +  + D +  A  S +
Sbjct:  413 KSMLLSCSEDGTIRLW--------ICYRGHMYAVWHVKF-SPQGYYFASAGHDKTVMLWSTEQHHSLRIFAGHFSDVDCIAFHP-NCNYIASGSSDRSVRVWDCVS-GNCVRLMTGHKTAVSTLAFSPDGRFLASGGRDSRVLFWDIAHGHLLADLSNHSQTVTALAFSRDGNILAXSSTD 582          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000001730 (supercontig:LSalAtl2s:LSalAtl2s129:321159:324031:-1 gene:EMLSAG00000001730 transcript:EMLSAT00000001730 description:"maker-LSalAtl2s129-snap-gene-3.7")

HSP 1 Score: 62.3882 bits (150), Expect = 4.119e-10
Identity = 45/167 (26.95%), Postives = 76/167 (45.51%), Query Frame = 0

Query:  379 FHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFV 544
             H+NQ++S   D T+ +WD  T +       H      V +   D +L  S + D  VK+W+      + +L    N V S++F+     HV  GS D  +  +D+        +  GH+   S +   N   L+S + DS +K+WD+    C+Q+  GH   ++ V
Sbjct:  413 LHENQVVSGSRDSTLRIWDIETGECLHILAGHLAAVRCVQY---DGRLVVSGAYDYMVKVWNPEREECLHTLSGHTNRVYSLQFDG---IHVVSGSLDTSIRVWDVETGACKHALM-GHQSLTSGMELRNNI-LVSGNADSTVKVWDITNGQCLQTLSGHNKHQSAV 571          

HSP 2 Score: 58.9214 bits (141), Expect = 6.019e-9
Identity = 47/200 (23.50%), Postives = 86/200 (43.00%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFH-----VAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            I+C+    F  N+++S   D T+ +W A T +  ++ V H    WS         +  S S D  +K+W+      + +L    +          C H     V  GS D  +  +D+   +  L +  GH  AV  V++ +   ++S + D  +K+W+ E++ C+ +  GH N      L  D  +   GS + S  ++
Sbjct:  328 ITCLQ---FSNNRIVSGSDDNTLKVWSATTGKCLRTLVGHTGGVWSXQMRD---NIIVSGSTDRSLKVWNADAGTCVHTLYGHTST-------VRCMHLHENQVVSGSRDSTLRIWDIETGEC-LHILAGHLAAVRCVQY-DGRLVVSGAYDYMVKVWNPEREECLHTLSGHTNR--VYSLQFDGIHVVSGSLDTSIRVW 510          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000011537 (supercontig:LSalAtl2s:LSalAtl2s799:381305:384324:1 gene:EMLSAG00000011537 transcript:EMLSAT00000011537 description:"maker-LSalAtl2s799-snap-gene-3.77")

HSP 1 Score: 60.4622 bits (145), Expect = 1.280e-9
Identity = 63/266 (23.68%), Postives = 110/266 (41.35%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL---------NATELL-SASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            I  + WN    N +LS+  D T  +WD++T    + +  H      VD+ + ++  FAS S D  + +    V   I S     N V ++K++P   F +A  S D  +  + + + +S +     H K +  +++          NA  +L SAS DS ++LWDVE+ +C+     H      V  + D  + A GS +    ++     K + S+K                     +  +CW    D +  + S G V +  L
Sbjct:  236 IFALKWNK-SGNYILSAGVDRTTIIWDSQTGNHKQQFAFHSAPALDVDWQTDES--FASCSTDKCIHVCKLGVEKPIKSFYGHTNEVNAIKWDPQGKF-LASCSDDMTLKVWCM-DKESHVHDLQAHSKEIYTIKWSCTGPGTNNPNADLVLASASFDSTVRLWDVERGSCLHXLXRHKEPVYSVAFSPDGKFLASGSFDKCVHIWSTVTGKLVHSYKGTGG-----------------IFEVCWNSRGDKVGASASDGTVFVLDL 479          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|55976539|sp|Q8NHY2.1|RFWD2_HUMAN (RecName: Full=E3 ubiquitin-protein ligase RFWD2; AltName: Full=Constitutive photomorphogenesis protein 1 homolog; Short=hCOP1; AltName: Full=RING finger and WD repeat domain protein 2; AltName: Full=RING finger protein 200; AltName: Full=RING-type E3 ubiquitin transferase RFWD2)

HSP 1 Score: 541.576 bits (1394), Expect = 9.629e-179
Identity = 287/626 (45.85%), Postives = 405/626 (64.70%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNAL---AEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLS--QANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDL---------------ESTLKH------AHRLGVD-------PEVSSDSALRKE----SPLSARMQSMHQHYPNLVDCYFETRRSVASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWR---KGTDNI-IGANSQGCVKIYRL 625
            +++CPICF++ +EA +TKCGHSFC +C+  +LE  +++CPKC  V+++   + PNF +N L    +Q    ++  L   +SS                        H      D+L   Q NL   ++  +   L Q K+ +      +++ +L EFL V ++ K+E L + +++L ++  D+               +ST+        +H   +D       P  S  S  +K+    S L++R + +  H+ +L  CYF TR S  S    D  R     L++F   LSKFT+Y+ +R +A L+Y SD+Y+ +SIVSSIEFD+D ++FAIAGVTK+IKVY+Y+ VI+D VDIHYP  EM  +SKISC+SW+S+HKN L SSDY+GTV LWD  T Q++K Y EHEKRCWSVDFN +D KL AS SDDA+VKLWST++++S+AS+EAKANVC VKF+P+S +H+AFG ADHCVHY+DLRN K P+ VF GH+KAVSY +F++  E++SASTDSQLKLW+V K  C++S++GHINEKNFVGL S+ DY A GSENNS +L+YKGLSK L +FKFD  +++  + D+  D++NEFVSA+CWR    G  N+ I ANSQG +K+  L
Sbjct:  133 DFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLE-DNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNG---------------------HRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRIS----DDSR-TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDK-DRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLEL 730          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|55976616|sp|Q9R1A8.2|RFWD2_MOUSE (RecName: Full=E3 ubiquitin-protein ligase RFWD2; AltName: Full=Constitutive photomorphogenesis protein 1 homolog; Short=mCOP1; AltName: Full=RING finger and WD repeat domain protein 2; AltName: Full=RING-type E3 ubiquitin transferase RFWD2)

HSP 1 Score: 540.036 bits (1390), Expect = 4.083e-178
Identity = 285/622 (45.82%), Postives = 404/622 (64.95%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDL---------------ESTLKH------AHRLGVD-------PEVSSDSALRKE----SPLSARMQSMHQHYPNLVDCYFETRRSVASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDN----IIGANSQGCVKIYRL 625
             +++CPICF++ +EA +TKCGHSFC +C+  +LE  +++CPKC  V+++   + PNF +N L  +     +     L  S  + + H            +   + G  Q  D L  AN++      +   L Q K+ +      +++ +L EFL V ++ K+E L + +++L ++  D+               +ST+        +H   +D       P  S  S  +K+    S L++R + +  H+ +L  CYF TR S  S    D  R     L++F   LSKFT+Y+ +R +A L+Y SD+Y+ +SIVSSIEFD+D ++FAIAGVTK+IKVY+Y  VI+D VDIHYP  EM  +SKISC+SW+S+HKN L SSDY+GTV LWD  T Q++K Y EHEKRCWSVDFN +D KL AS SDDA+VKLWST++++S+AS+EAKANVC VKF+P+S +H+AFG ADHCVHY+DLRN K P+ VF GH+KAVSY +F++  E++SASTDSQLKLW+V K  C++S++GHINEKNFVGL S+ DY A GSENNS +L+YKGLSK L +FKFD  +++  + D+  D++NEFVSA+CWR  +D     +I ANSQG +K+  L
Sbjct:  134 NDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLE-DNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHR---------WQIFQDLLGTDQ--DNLDLANVNL-----MLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGTSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRIS----DDSR-TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDK-DRKEDDTNEFVSAVCWRALSDGESNVLIAANSQGTIKVLEL 732          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|13124294|sp|Q9UJM8.1|HAOX1_HUMAN (RecName: Full=Hydroxyacid oxidase 1; Short=HAOX1; AltName: Full=Glycolate oxidase; Short=GOX)

HSP 1 Score: 406.371 bits (1043), Expect = 7.636e-132
Identity = 196/356 (55.06%), Postives = 256/356 (71.91%), Query Frame = 0

Query:  669 MACLDDIEAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRDLV 1024
            + C++D E HA  VLP++  DYYRSGA+  +TL DN  AF R+++ P+ML +V+  DLS SVLG+ V+ PI +  TAMQRMAH +GE A+ RA Q +GT  +LS+ ATSSIEEVA+A P A +W QLYIYKDREVTK LV +AEK GY+A+ +TVD P+ G RL D+RN+F+LP  L M NF       +     G  SG+  YVA   D ++SW+D+ WL+  + LPI+ KGILR DDA   +K+G + I+VSNHGARQLDGV ATIDVL  I   V  + E++LDGGVR GTDVLKALALGAK VF+GRP++WGLA+ G+KGV+  L ++++E   AMALSGC +V  +D+ LV
Sbjct:    5 LICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLV 360          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|13124296|sp|Q9WU19.1|HAOX1_MOUSE (RecName: Full=Hydroxyacid oxidase 1; Short=HAOX1; AltName: Full=Glycolate oxidase; Short=GOX)

HSP 1 Score: 404.06 bits (1037), Expect = 6.251e-131
Identity = 196/357 (54.90%), Postives = 256/357 (71.71%), Query Frame = 0

Query:  669 MACLDDIEAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLA-TKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRDLV 1024
            + C+ D E H   VL ++  DYYRSGA+  +TL DN +AF R+++ P+ML +V+  DLS SVLG+ V+ PI +  TAMQ MAH +GE A+ RA Q MGT  +LS+ ATSSIEEVA+A P A +W QLYIYKDRE+++ +V RAEK GY+A+ +TVD P+ G R+ D+RN+F+LP  L M NF    DLA +  G  G  SG+  YVA   D +LSW D+ WL+  + LPI++KGILR DDA   +K+G   I+VSNHGARQLDGV ATIDVL  I   V  + E++LDGGVR GTDVLKALALGAK VF+GRP+IWGLA+ G+KGV+  L ++++E   AMALSGC +V  +D+ LV
Sbjct:    5 LVCISDYEQHVRSVLQKSVYDYYRSGANDQETLADNIQAFSRWKLYPRMLRNVADIDLSTSVLGQRVSMPICVGATAMQCMAHVDGELATVRACQTMGTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNF-ETNDLAFSPKGNFGDNSGLAEYVAQAIDPSLSWDDITWLRRLTSLPIVVKGILRGDDAKEAVKHGVDGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIIWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLV 360          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|20141387|sp|P43254.2|COP1_ARATH (RecName: Full=E3 ubiquitin-protein ligase COP1; AltName: Full=Constitutive photomorphogenesis protein 1; AltName: Full=RING-type E3 ubiquitin transferase COP1)

HSP 1 Score: 404.445 bits (1038), Expect = 6.159e-127
Identity = 235/662 (35.50%), Postives = 358/662 (54.08%), Query Frame = 0

Query:   43 LCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQA-NLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLESTLKHA--------------HRLGVDPEV---------------------------------------SSDSALRKESPLS-ARMQSMHQ-------------HYPNLVDCYFETRRSVASEPLGDSPRPN----------PGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRLG 626
            LCPIC ++ K+A +T CGHSFC  C++  L + S  CP C Q + +  ++ PNF L+ L               L    AR  H +  AS             L Q  + L +  ++S K++++L   L + KR + + EA   + +L +FL+ L+K+K + L + + DL  +  D+ +  +H                 LG DP                                          S   L K+  LS +  QS++Q              + +L +CY + RR +A +P  +S + N            GL  F + L+ FT+YS +RV+A + +  D++ +A+IVSSIEFD+D E FA AGV++ IKV+D++ V+ +  D+  P  EM + SK+SC+SWN   KN + SSDY+G VT+WD  T Q    Y EHEKR WSVDF+  +  +  S SDD +VK+W T    S+ +++ KAN+C VK+NP S  ++A GSADH +HY+DLRN+  PL VF GHKKAVSYV+FL+  EL SASTDS L+LWDV+    +++++GH NEKNFVGLT + +Y A GSE N  ++++K +++P+ S +F +       +D   +  + F+SA+CW+  +  ++ ANSQG +K+  L 
Sbjct:   51 LCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSD-CPCCSQHLTN-NQLYPNFLLDKL---------------LKKTSAR--HVSKTASP------------LDQFREALQRGCDVSIKEVDNLLTLLAERKRKMEQEEAERNMQILLDFLHCLRKQKVDELNEVQTDLQYIKEDINAVERHRIDLYRARDRYSVKLRMLGDDPSTRNAWPHEKNQIGFNSNSLSIRGGNFVGNYQNKKVEGKAQGSSHGLPKKDALSGSDSQSLNQSTVSMARKKRIHAQFNDLQECYLQKRRQLADQP--NSKQENDKSVVRREGYSNGLADFQSVLTTFTRYSRLRVIAEIRH-GDIFHSANIVSSIEFDRDDELFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLSCLSWNKHEKNHIASSDYEGIVTVWDVTTRQSLMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQEASVINIDMKANICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLHVFSGHKKAVSYVKFLSNNELASASTDSTLRLWDVKDNLPVRTFRGHTNEKNFVGLTVNSEYLACGSETNEVYVYHKEITRPVTSHRFGSP----DMDDAEEEAGSYFISAVCWKSDSPTMLTANSQGTIKVLVLA 674          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|75318383|sp|O49506.1|GLO5_ARATH (RecName: Full=Peroxisomal (S)-2-hydroxy-acid oxidase GLO5; AltName: Full=Glycolate oxidase 3; Short=AtGLO5; Short=GOX 3; AltName: Full=Short chain alpha-hydroxy acid oxidase GLO5)

HSP 1 Score: 360.533 bits (924), Expect = 2.083e-114
Identity = 183/346 (52.89%), Postives = 240/346 (69.36%), Query Frame = 0

Query:  676 EAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDR 1021
            E  A + LP+   DYY SGA+   TL +N+ AF R    P++L+DVS+ D+S +VLG +++ PI IAPTAMQ+MAHP+GE A+ARA    GTI  LS+ AT S+EEVA   P  R +FQLY+YKDR V   LV RAE+AG++A+ LTVD P  GRR +DI+N+F LP  L + NF GL DL     T+   SG+ +YVA   DQ+LSWKD+ WL+S + LPI++KG++  +DA   ++YGA+ IIVSNHGARQLD V ATI  L  +   V  R  ++LDGGVR GTDV KALALGA  VF+GRP ++ LA DG+ GV+K L ++RDE +  MALSGC S+  + R
Sbjct:   10 EKIAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDVSTTVLGFNISMPIMIAPTAMQKMAHPDGELATARATSAAGTIMTLSSWATCSVEEVASTGPGIR-FFQLYVYKDRNVVIQLVKRAEEAGFKAIALTVDTPRLGRRESDIKNRFALPRGLTLKNFEGL-DLGKIDKTND--SGLASYVAGQVDQSLSWKDIKWLQSITSLPILVKGVITAEDARIAVEYGAAGIIVSNHGARQLDYVPATIVALEEVVKAVEGRIPVFLDGGVRRGTDVFKALALGASGVFVGRPSLFSLAADGEAGVRKMLQMLRDEFELTMALSGCRSLREISR 351          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|3121867|sp|P93471.1|COP1_PEA (RecName: Full=E3 ubiquitin-protein ligase COP1; AltName: Full=Constitutive photomorphogenesis protein 1; AltName: Full=RING-type E3 ubiquitin transferase COP1)

HSP 1 Score: 369.777 bits (948), Expect = 7.261e-114
Identity = 182/405 (44.94%), Postives = 261/405 (64.44%), Query Frame = 0

Query:  231 DPEVSSDSALRKESPLS-ARMQSMHQHYPNLVDCYFETRRSVASEPLGDSPRPN--------PGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRLG 626
            DP   SDS    +S L+  R + +H  + +L +CY + RR  A +P G   R            GL+ F + L+ FT+YS +RV+A + +  D++ +A+IVSSIEFD+D + FA AGV++RIKV+D++ V+ +  D H P  EM + SK+SC+SWN + KNQ+ SSDY+G VT+W   T +    Y EHEKR WSVDF+  D  +  S SDD +VK+W T+   S+ +++ KAN+C VK+NP S  ++A GSADH +HY+DLRN+  P+ VF GHKKAVSYV+FL+  EL SASTDS L+LWDV++   +++++GH NEKNFVGLT   +Y A GSE N  F+++K +SKPL   +F          D   +  + F+SA+CW+     I+ ANSQG +K+  L 
Sbjct:  272 DPITGSDSQYINQSGLALVRKKRVHTQFNDLQECYLQKRRQAADKPHGQQERDTNFISREGYSCGLDDFQSVLTTFTRYSRLRVIAEIRH-GDIFHSANIVSSIEFDRDDDLFATAGVSRRIKVFDFSAVVNEPTDAHCPVVEMTTRSKLSCLSWNKYAKNQIASSDYEGIVTVWTMTTRKSLMEYEEHEKRAWSVDFSRTDPSMLVSGSDDCKVKVWCTNQEASVLNIDMKANICCVKYNPGSGNYIAVGSADHHIHYYDLRNISRPVHVFTGHKKAVSYVKFLSNDELASASTDSTLRLWDVKQNLPVRTFRGHANEKNFVGLTVRSEYIACGSETNEVFVYHKEISKPLTWHRFGT----LDMEDAEDEAGSYFISAVCWKSDRPTILTANSQGTIKVLVLA 671          

HSP 2 Score: 72.4034 bits (176), Expect = 1.273e-11
Identity = 121/576 (21.01%), Postives = 224/576 (38.89%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLESTLKH-------AHRLGVDPEVSSDSALRKESPLSARMQSMHQHYPNLVDCYFETRRSVASEPLGDSPRPNPG---------GLNQ---FSTSLSKFTKYSGI------RVMANLNYTSDMY----SNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWS--------TSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRF--LNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF-YKGLSKPLFSF 576
            ++LCPIC ++ K+A +T CGHSFC  C++  L + S  CP C   + ++  + PNF L+ L ++  + Q S  A+                          PV    Q V    +  +   D   L   L + KR + + EA   + +L +FL+ L+K+K + L + + DL  +  D+ +  KH         R  V   +  DS  RK     +R  SM  +   L       R  ++S   G   + N G         G+ +    + S S++   SG+      RV    N   + Y      A+     + ++D  F +  G +    + D+  V+       Y R  +I+  +      + FH   ++S                             S++F+  D  LFA+A    R+K++         T  +  +  +  ++ +  + +N  +   +A    +  V  + +   KS L  +  H+K    V F   + + L+S S D ++K+W   ++  + +     N           +Y A GS ++    +  + +S+P+  F
Sbjct:   44 DFLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSD-CPCCGHYLTNSN-LFPNFLLDKLLKKTSDRQISKTAS--------------------------PVEHFRQAVQKGCEVTMKELDTLLLL--LTEKKRKMEQEEAERNMQILLDFLHCLRKQKVDELKEVQTDLQFIKEDIGAVEKHRMDLYRARDRYSVKLRMLDDSGGRK-----SRHSSMDLNSSGLASSPLNLRGGLSS---GSHTKKNDGKSQISSHGHGIQRRDPITGSDSQYINQSGLALVRKKRVHTQFNDLQECYLQKRRQAADKPHGQQERDTNFISREGYS--CGLDDFQSVLTTFT--RYSRLRVIAEIR----HGDIFHSANIVS-----------------------------SIEFDR-DDDLFATAGVSRRIKVFDFSAVVNEPTDAHCPVVEMTTRSKLSCLSWNKYAKNQIASSDYEGIVTVWTMTTRKS-LMEYEEHEKRAWSVDFSRTDPSMLVSGSDDCKVKVWCTNQEASVLNIDMKANICCVKYNPGSGNYIAVGSADHHIHYYDLRNISRPVHVF 542          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|121530|sp|P05414.1|GOX_SPIOL (RecName: Full=Peroxisomal (S)-2-hydroxy-acid oxidase; AltName: Full=Glycolate oxidase; Short=GOX; AltName: Full=Short chain alpha-hydroxy acid oxidase)

HSP 1 Score: 353.984 bits (907), Expect = 7.523e-112
Identity = 182/350 (52.00%), Postives = 244/350 (69.71%), Query Frame = 0

Query:  672 LDDIEAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDR 1021
            +++ EA A + LP+   DYY SGA+   TL +N+ AF R    P++L+DV+  D++ ++LG  ++ PI IAPTAMQ+MAHPEGE A+ARAA   GTI  LS+ ATSS+EEVA   P  R +FQLY+YKDR V   LV RAE+AG++A+ LTVD P  GRR ADI+N+F LP  L + NF G+ DL          SG+++YVA   D++LSWKDVAWL++ + LPI++KG++  +DA   +++GA+ IIVSNHGARQLD V ATI  L  +      R  ++LDGGVR GTDV KALALGA  VF+GRPV++ LA +G+ GVKK L ++RDE +  MALSGC S+  + R
Sbjct:    6 VNEYEAIAKQKLPKMVYDYYASGAEDQWTLAENRNAFSRILFRPRILIDVTNIDMTTTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIR-FFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGI-DLGKM--DKANDSGLSSYVAGQIDRSLSWKDVAWLQTITSLPILVKGVITAEDARLAVQHGAAGIIVSNHGARQLDYVPATIMALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAAGVFIGRPVVFSLAAEGEAGVKKVLQMMRDEFELTMALSGCRSLKEISR 351          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|75326731|sp|Q7FAS1.1|GLO3_ORYSJ (RecName: Full=Peroxisomal (S)-2-hydroxy-acid oxidase GLO3; AltName: Full=Glycolate oxidase 3; Short=GOX 3; Short=OsGLO3; AltName: Full=Short chain alpha-hydroxy acid oxidase GLO3 >gi|317376201|sp|B8AUI3.1|GLO3_ORYSI RecName: Full=Peroxisomal (S)-2-hydroxy-acid oxidase GLO3; AltName: Full=Glycolate oxidase 3; Short=GOX 3; Short=OsGLO3; AltName: Full=Short chain alpha-hydroxy acid oxidase GLO3)

HSP 1 Score: 353.214 bits (905), Expect = 1.106e-111
Identity = 184/354 (51.98%), Postives = 249/354 (70.34%), Query Frame = 0

Query:  672 LDDIEAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRDLVI 1025
            + + E  A + LP+   DYY SGA+   TL +N++AF R    P++L+DVSR +++ +VLG +++ PI IAP+AMQ+MAHPEGE A+ARAA   GTI  LS+ +TSS+EEV  AAP  R +FQLY+YKDR + + LV RAE AG++A+ LTVD P  GRR ADI+N+F LP HL + NF  L DL     T+   SG+ +YVAS  D++LSW DV WL++ + LPI++KG++  +D    ++ GA+ IIVSNHGARQLD V ATI  L  +      R  ++LDGGVR GTDV KALALGA  VF+GRPV++ LA DG+ GV+K L ++RDEL+  MALSGC S++ + R+ VI
Sbjct:    7 VSEYEQLAKQKLPKMIYDYYASGAEDQWTLKENREAFSRILFRPRILIDVSRINMATNVLGFNISMPIMIAPSAMQKMAHPEGELATARAASAAGTIMTLSSWSTSSVEEVNSAAPGIR-FFQLYVYKDRNIVRQLVRRAELAGFKAIALTVDTPRLGRREADIKNRFNLPPHLVLKNFEAL-DLGKMDKTND--SGLASYVASQVDRSLSWTDVKWLQTITSLPILVKGVMTAEDTRLAVESGAAGIIVSNHGARQLDYVPATISCLEEVVREAKGRLPVFLDGGVRRGTDVFKALALGASGVFIGRPVLFSLAVDGEAGVRKVLQMLRDELELTMALSGCTSLAEITRNHVI 356          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|13124262|sp|Q9LRR9.1|GLO1_ARATH (RecName: Full=(S)-2-hydroxy-acid oxidase GLO1; AltName: Full=Glycolate oxidase 1; Short=AtGLO1; Short=GOX 1; AltName: Full=Short chain alpha-hydroxy acid oxidase GLO1)

HSP 1 Score: 353.214 bits (905), Expect = 1.141e-111
Identity = 180/349 (51.58%), Postives = 244/349 (69.91%), Query Frame = 0

Query:  674 DIEAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRD 1022
            + +A A + LP+   DYY SGA+   TL +N+ AF R    P++L+DVS+ D++ +VLG  ++ PI +APTAMQ+MAHP+GE A+ARAA   GTI  LS+ ATSS+EEVA   P  R +FQLY+YK+R V + LV RAE+AG++A+ LTVD P  GRR +DI+N+F LP +L + NF GL DL          SG+ +YVA   D+TLSWKDV WL++ +KLPI++KG+L  +DA   ++ GA+ IIVSNHGARQLD V ATI  L  +      R  ++LDGGVR GTDV KALALGA  +F+GRPV++ LA +G+ GV+K L ++RDE +  MALSGC S+  + R+
Sbjct:    8 EYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMTTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIR-FFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGL-DLGKM--DEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIAIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRN 352          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1026572726|gb|OAD59875.1| (E3 ubiquitin-protein ligase RFWD2 [Eufriesea mexicana])

HSP 1 Score: 587.03 bits (1512), Expect = 0.000e+0
Identity = 303/622 (48.71%), Postives = 408/622 (65.59%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGAS-STPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAHRLGVDPE---------------------VSSDSALRKE-------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+ AG   S P      P  GL   +   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + L+      +  E                     V SD+ +          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  + ++A LNY+SD+Y+N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFD  R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:  119 DYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCGYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYTADGRHKVAGTDLSVP------PYDGLRDIIAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMEEVENILRDVQNKSIRKEMIGLIDIIDSNMVKPNEKASVVGSDTFINPTGSKNAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELHLGHKSQSQSDSWQGTNSGLDVFRENLVKFSRYKSLNLLATLNYSSDIYNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHHKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDTVRSILEMQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 730          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1101362169|ref|XP_018906743.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like isoform X1 [Bemisia tabaci])

HSP 1 Score: 583.948 bits (1504), Expect = 0.000e+0
Identity = 305/635 (48.03%), Postives = 413/635 (65.04%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACS-KVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDL---ESTLKHAHRLGVDPE--------------VSSDSALRKES-----------------PLSARMQSMHQHYPNLVDCYFETR----------RSVASEPLGDSPRPNPG---GLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRLG 626
             +YLCPICFE+ +EA +TKCGH+FC +C+  +LE  +++CPKC  +I     + PNF LN L  +                + R+  +  G S   S   L P+    +        NL+  D+  +   L Q ++++ E E+C+ +  LL EFL  L K K+  L +  +++ ++  D+   ES L      G+D E                SD+ +  +S                  LS R + MH H+ + V CYF  R          +S   E   D P  + G   GL+QF  +L KF+++SG+R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKVYDY  VIRD+VDIHYP  EM+S+SKISC+SWNS+HKN L SSDY+GTVT+WD  T Q+T+++ EHEKRCWSVDFN V+ +L AS SDDARVKLWS  V+HS+ASLEAKANVC V+FNP S  H+AFGSADHCVHY+DLRN+K+PL +F GH+KAVSYV+FLN  +++SASTDSQLKLW++    C++S  GHINEKNFVGL ++EDY A GSENN+ +++YKGL+K LFSFKFDA R++  + D+  DE+NEFVSA+CWR+ ++ ++ ANSQG +KI  L 
Sbjct:   38 NDYLCPICFELIEEAHITKCGHTFCFQCISKSLE-GNNRCPKCNSMIGGREHIFPNFLLNELITKY-------------KTKVRNLKDLVGGSDKVSDDPLPPLSDNLRGFVASESQNLALADVNVILEVLNQ-RKSILEAESCAVQNKLLHEFLKHLLKHKEAQLNQLTKEVSLIKRDINEVESILSTIDVKGIDLEQGSFDQEDFPPSDKAKSDAGMSCDSVESFNGSQKNSNNLTIGSLSMRRKRMHAHFNDFVRCYFNLRAKELLFSKNDKSPVPEKDKDKPLGSSGPSSGLDQFRDNLVKFSRFSGLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVYDYGAVIRDIVDIHYPCIEMLSNSKISCLSWNSYHKNTLASSDYEGTVTVWDVNTGQRTRTFQEHEKRCWSVDFNKVNIRLIASGSDDARVKLWSLDVDHSVASLEAKANVCCVQFNPGSSNHLAFGSADHCVHYYDLRNMKAPLALFEGHRKAVSYVKFLNNEDIVSASTDSQLKLWNINTSYCLRSLVGHINEKNFVGLAANEDYIACGSENNALYVYYKGLTKQLFSFKFDAVRSVLEK-DKKDDEANEFVSAVCWRQLSNVVVAANSQGIIKILELA 656          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|915669485|gb|KOC71003.1| (E3 ubiquitin-protein ligase RFWD2 [Habropoda laboriosa])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 304/627 (48.48%), Postives = 411/627 (65.55%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGAS-STPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAHRLGVDPE---------------------VSSDSAL-----RKE------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFE+  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H A G   S P      P  GL   V   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + L+      +  E                     V SD+ +     +K+      S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNFVGL +D DY A GSENN+ +++YKGL+K LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   50 NDYLCPICFEIIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGRHRAVGTDLSVP------PYDGLRDIVAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMEEVENILRDVQNKSIRKEMIGLIDIIDSNMVKPNEKASAVGSDTFVNPTGSKKQNDYAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATGQRTKAFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNLHCLRSFVGHVNEKNFVGLATDGDYVACGSENNALYIYYKGLTKQLFSYKFDAVRSILEIPERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 666          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1059211601|ref|XP_017752844.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Eufriesea mexicana])

HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 304/642 (47.35%), Postives = 411/642 (64.02%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGAS-STPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAH---------RLGVDPEVSSDSALRKE--------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+ AG   S P      P  GL   +   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + L+            +   + + +  SA+RKE                                      S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  + ++A LNY+SD+Y+N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFD  R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   68 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCGYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYTADGRHKVAGTDLSVP------PYDGLRDIIAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMEEVENILRDVQNKCPKVEDLKKSSENDTAQVSAIRKEMIGLIDIIDSNMVKPNEKASVVGSDTFINPTGSKNAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELHLGHKSQSQSDSWQGTNSGLDVFRENLVKFSRYKSLNLLATLNYSSDIYNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHHKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDTVRSILEMQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 699          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|925675420|gb|KOX72753.1| (E3 ubiquitin-protein ligase RFWD2 [Melipona quadrifasciata])

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 300/625 (48.00%), Postives = 409/625 (65.44%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAHRLGVDPE--------------------VSSDSAL-----RKE------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFE+  EA +T+CGH+FC  C+V +LE  + +CPKC  ++ +  ++ PNF L+ L  +     K GL  L SS  A   H+       P      P  GL   +   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + LK      +  E                    V SD+ +     +K+      S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+G VT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:  118 NDYLCPICFEIIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYML-TQQDIFPNFLLHELISKYKTRIK-GLTELGSSYAADGRHKIGIDLPVP------PHDGLRDIIAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKKDMEEVENILKDVQNKSIRKEMIGLIDIIDSNMVKPNEKANVGSDTFINPTGSKKQNDYAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKALRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGIVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 732          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|970889350|ref|XP_015109882.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Diachasma alloeum])

HSP 1 Score: 579.326 bits (1492), Expect = 0.000e+0
Identity = 302/630 (47.94%), Postives = 407/630 (64.60%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAH---------RLGVDPEVSSDSALRKE---------------------------SPLSARMQSMHQHYPNLVDCYFETRRS-----VASEPLG-DSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            +YLCPICFEV  EA +T+CGH+FC +C+  TLE +  +CPKC   +    ++ PNF LN L  +     K G+     +       E+   SS P      P  GL   V   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   S LK            +   + E +  SA+RKE                           S L+ R + MH H+ + V CYF+ R       V   P+  D+      GL+ F  +L KF++Y+ +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY  VIRD VDIHYP  EM+S SKISCVSWNS+HK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDT+L AS SDDARVKLWS +++HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNFVGL +D DY A GSENN+ +++YKGL+K LFS+KFDA RT+   +++  ++ NEFVSA+CWR+ ++ ++ ANSQG +KI  L
Sbjct:   42 DYLCPICFEVIDEAHITRCGHTFCYKCITKTLE-SLGRCPKCNFTLGQ-QDIFPNFLLNELVSKYKTRIK-GIGEFGCAHGDGRRRESGSESSVP------PCDGLRDFVAAES-ANLTLPDVNVMLEVLTQRKLLLEAESCAAQNKLLHEFLKHLLQQKEEQRNQLQKEVALIKRDMEEVESILKDVQSKCPRIEDLQRTRENETAQMSAIRKEMIDLIDIIDSNMVKHDENLQKPEHTTVSTLAVRRKRMHAHFEDFVQCYFDARAKDLLLGVPKNPVNIDTVHGMSSGLDVFRDNLVKFSRYNSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYGAVIRDTVDIHYPCIEMVSSSKISCVSWNSYHKGMLASSDYEGTVTVWDAATGQRTKAFQEHEKRCWSVDFNDVDTRLIASGSDDARVKLWSLNIDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKVWNINNPHCLRSFVGHVNEKNFVGLATDGDYVACGSENNALYVYYKGLTKQLFSYKFDAVRTVLELHERREEDPNEFVSAVCWRQMSNVVVAANSQGIIKILEL 661          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1241833515|gb|PBC29833.1| (E3 ubiquitin-protein ligase [Apis cerana cerana])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 305/645 (47.29%), Postives = 409/645 (63.41%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE-----------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K G A L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                         S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:  125 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GFAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANISSDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 759          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|820855890|ref|XP_012345148.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Apis florea])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 306/646 (47.37%), Postives = 410/646 (63.47%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE------------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   69 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANVISSDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 704          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|572262111|ref|XP_006609321.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Apis dorsata])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 306/646 (47.37%), Postives = 410/646 (63.47%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE------------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   69 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANIISSDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 704          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1032012470|ref|XP_016772257.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Apis mellifera])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 306/646 (47.37%), Postives = 410/646 (63.47%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE------------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   69 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANIISNDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 704