basement membrane-specific heparan sulfate proteoglycan core protein, maker-scaffold48_size466083-snap-gene-1.19 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000003993 (supercontig:LSalAtl2s:LSalAtl2s213:441137:447252:-1 gene:EMLSAG00000003993 transcript:EMLSAT00000003993 description:"maker-LSalAtl2s213-snap-gene-4.21") HSP 1 Score: 167.162 bits (422), Expect = 9.637e-43 Identity = 179/740 (24.19%), Postives = 294/740 (39.73%), Query Frame = 0
Query: 38 PHFNQAPLSYMELVTLPDSYLN----LDLELSFKPELANGLILYNAHDERG----GKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQW-HTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTP-LYLGGTPSQVQAL--EDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKS-----QGVDTC--DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTG---ACNGGRCQNTAK-DFECFCPFGQEGSFCEDTTTIERPAFQGN-SYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQG-DFASVTIQDGHLEFRFNTGS------GTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKV--IVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQH---TCQCPEDFRGDNCERRATQCDISMPCQNGGRCE-GGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGS-AELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSR---LTHGRYVYGFRGCLHRLRIQGREIDFQRDPVSTG 736
P F + Y + P N L+ + F + + L +RG G DFI+ G + ++LG+ + ++P+ + H V+ R+ + L VD+ I G +PG+ E L++ + Y+GG + +L D ++ GF G + + +V G + P+ + S + V+ C D CS SPC NGG C G + C C G G C + C TG +C T K +EC CPFG+ P F G+ SY+ I + R T + +++ QS +G+ DF S+ + +G + F F+ G G + + KWY V R + G + GQ G + L++ ++ GG+ V + GCI ++ K+ L+ NI+DC T C CQNGG C ++ H C CP+++ G++CE+ + C+N G C G E C CP G+ G C + F GD F + F +E + ++ +++ + + G +NLG S ELT +ND K R+ + R ++ + +DG+ + + G L+T + IGG + +T G Y GF GC+ + IQ +D S+G
Sbjct: 790 PSFRSSVGGYSSYLAYPVHTQNVLKSLEWQFHFSTPNTDQVALLFFLGQRGIHDFGSDFIALSYIKGHILMTWDLGAGPRRIFTKRPVDERYFVHAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVTSMYYYIGGHENYNFSLLPHDFPVHRGFSGCMFDMAY---RSYVTGQLTPLALPSKIGSSPIRGRNVEQCHYDICSSSPCSNGGTCIGXG--ASFLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGSYECICPFGK------------NPLFTGHGSYMQTHLKSAIRFNTHIHIEIRP-QTLEGHIIHLGQSRNGRHQDFLSLLLVNGSVVFSFSLGGPIGGNVGNTVTIRTCCVEIDKWYSVEAGRYGRKGYLRXDGQ-FTTGSTESGLNTLDVDQELFLGGIPNSHMLFIDFVSKHY-YEGCIRNLHINEKSYPLTSTGNHWKGWNIKDCDGT--ACGGELCQNGGKCSLSENIHNHRGYECGCPDNYIGEHCEQHRLCIN---ACENDGICSIGDDEIIHCDCPLGYHGSFCSDEEEIRVPR---FYGDGF--------------------ISFVLSETK-------SIRHDQTSDDYLGVGLKDGMVHLVWNLGWFSRTELTVPSKVNDDKWHRVLIDRIRQELKLDVDGK-IYQSRVTGSYHELNTVNVVLIGGSSEKDNVQDMTRGHYSMGFNGCMRNVTIQSSSMDKALKQKSSG 1473 HSP 2 Score: 152.14 bits (383), Expect = 6.425e-38 Identity = 177/742 (23.85%), Postives = 307/742 (41.37%), Query Frame = 0
Query: 59 NLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPIT---FGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGL-DLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDS--CSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACN-GGRC--QNTAKDFECFCPFGQEGSFCEDTTTIE---------------------RPAFQG-----NSYLAIP-RPKHILRQLKMAFRFRAVDTRDSVVLYSAQS---EDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKW-YEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYF-GGVNKEKYTLAPG-VGTDLGFIGCITDIRF-----GRKT-LDLSKVI----VDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISM-PCQNGGRCEGGTE-FYKCFCPWGFGGRNCEKVLPMNASTSVGFRGD-SFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLG------EGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV--YGFRGCLHRLRIQGREIDFQRDPVSTGN 737
N ++LSF+ GLIL+ D G +S +K+G + + G + + + + + Q ++ I S + + S + + L + LY+G P + + L + +N GF+G C+ + + E+ L + + + C S C++ PC NG IC + + C C PGF+G C+R +C T C GG C T F C C G+ GS CE+ I+ +P+F+ +SYLA P +++L+ L+ F F +T +L+ D DF +++ GH+ ++ G+G + + P+ + + + V R + G + + + G SPG LN+ + Y+ GG ++L P GF GC+ D+ + G+ T L L I + N+ C C +PC NGGTC + C+C + G C + C M C +C + Y+C CP+ G+N P+ F G S+M+ K F+ + +E +G I + G + + ++F++L + +G+ F F+LG G+ + + K ++ R RKG ++ DG+ GS++ L D +F+ GGI S + +V + + GC+ L I + STGN
Sbjct: 531 NFHVQLSFRTSQEEGLILFTGEDV--GYLLVSGYIKDGILTFKISCGHQIILFSDPRHRVDXGYLQXLSINITIFDTICSTSIQLNETRTMSGDQQIDSLPQIPDMLYMGLVPHK-KELGNGIINVGFQG--------CMKHLQLNSNELNLYRDAVDGRDIVECKSAICAIQPCRNGAICAQHEDSGSWFCDCPPGFTGALCER--PVCQTNPCQYGGTCLGSKTGSGFLCLCQAGRRGSLCEEGNQIQVYIKRLNYKAYEYFLPEVELTQPSFRSSVGGYSSYLAYPVHTQNVLKSLEWQFHFSTPNTDQVALLFFLGQRGIHDFGSDFIALSYIKGHILMTWDLGAGPRRIFTKRPVDERYFVHAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVTSMYYYIGGHENYNFSLLPHDFPVHRGFSGCMFDMAYRSYVTGQLTPLALPSKIGSSPIRGRNVEQCHY--DICSSSPCSNGGTCIGXGAS--FLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGSYECICPF---GKN-----PL-------FTGHGSYMQTHLKSAIRFNT----HIHIEIRPQTLEGHIIHL-GQSRNGRHQDFLSLLLVNGSVVFSFSLGGPIGGNVGNTVTIRTCCVEIDKWYSVEAGRYGRKGYLRXDGQ-FTTGSTESGLNTLDVDQELFL-GGIPNSHMLFIDFVSKHYYEGCIRNLHINEKSYPL----TSTGN 1229 HSP 3 Score: 115.546 bits (288), Expect = 1.819e-26 Identity = 130/521 (24.95%), Postives = 206/521 (39.54%), Query Frame = 0
Query: 261 MCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHIL---RQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYE-----VALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEG---GTEFYKCFCPWGFGGRNCEK------------------VLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQ----GLIWY--QDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLR-LKLIRKARKGSIQIDGRDLAKGSSKGFTGRLS-TDGNIFIGG--GIKPSRLTHGRYVY-GFRGCLHRLRIQGREIDFQRDPVSTGNLVP 740
+C + C NGG C T +C CP G CE+ IE P F G+S L K+ + R + FR + ++L++ ED S I+DG L F+ + G + P + + + + + + ++ ++ + G+ L M + G+ K L G+ ++GF GC+ ++ L+L + VD +I +C S + C PC+NG C + C CP F G CER Q + PCQ GG C G G+ F C C G G CE+ LP T FR + P ++++E +F + P L+++ Q G+ D F+ALS G ++LG G + + +++ + ++ R R+G +Q+D G S G L+ T +IGG S L H V+ GF GC+ D TG L P
Sbjct: 468 ICTSNVCSNGGTCLTTLSGVKCICPLQYHGVQCEEEVFIETPGFVGHSGLVHKLSKNNIVKDRNFHVQLSFRT-SQEEGLILFTG--EDVGYLLVSGYIKDGILTFKISCGHQIILFSDPRHRVDXGYLQXLSINITIFDTICSTSIQLNETRTMSGDQ--QIDSLPQIPDMLYMGLVPHKKELGNGI-INVGFQGCMKHLQLNSNELNLYRDAVDGRDIVECKS--AICAIQPCRNGAICAQHEDSGSWFCDCPPGFTGALCERPVCQTN---PCQYGGTCLGSKTGSGFL-CLCQAGRRGSLCEEGNQIQVYIKRLNYKAYEYFLPEVELTQPSFRSSVGGYSSYLAYPVHTQNVLKSLEWQFHFSTPNTDQVALLFFLGQRGIHDFGSD--FIALSYIKGHILMTWDLGAGPRRIFTKRPVDERYFVHAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVTSMYYYIGGHENYNFSLLPHDFPVHRGFSGCM---------FDMAYRSYVTGQLTP 965 HSP 4 Score: 58.9214 bits (141), Expect = 4.473e-9 Identity = 42/132 (31.82%), Postives = 63/132 (47.73%), Query Frame = 0
Query: 474 CSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQ------NGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTE 599
C++T ++ C NGG C G ++H C C + GD C+ +C IS PC NGG C G + + C CP + GR+CE P++ + +S + W + P + TK T +E ST E
Sbjct: 4 CNTTAAR----KCANGGHCIDGLG-EKHFCYCSSGWTGDECKENVDEC-ISNPCLHEGMCFNGGTCLDGVDDFTCSCPGDYMGRHCE--CPLDNPDNCINVNES---VSWVVDPSYLTTKYYTFNIETSTPE 124
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000004167 (supercontig:LSalAtl2s:LSalAtl2s220:203389:278765:1 gene:EMLSAG00000004167 transcript:EMLSAT00000004167 description:"maker-LSalAtl2s220-augustus-gene-2.5") HSP 1 Score: 125.946 bits (315), Expect = 6.819e-30 Identity = 109/427 (25.53%), Postives = 179/427 (41.92%), Query Frame = 0
Query: 243 RCIC----LPGFSGEHCK--REGEMCFTGACNGG-RCQNTAKDFECFCPFGQEGSFCE---DTTTIERPAFQG-NSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSE-----------------DGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGV----GTDLGFIGCITDIRFGRKTLDLSK----VIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRAT-QCDISMPCQNGGRCE----------GGTEFYKCFCPW----------GFGGRNCEK-----VLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQ 607
RCIC P + C+ + G +C C GG C F C+C G GS CE T + +F G +S++ I P ++++++ FR + ++ ++LY + V H+ F ++ GSG L+S I KW + R + G + + + V G + GS R LN++ ++ GGV+ + L V GT +GF+GCI +++ +KT+ L +++ + +C E+ C PC+N G C C C DF G +C+RR QCD + PCQN G C G+E+Y +C +G K L + S+G ++ MEL+ L DP + +++ + T GL+ Q
Sbjct: 506 RCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECE--ENSCSGNPCKNEGLCI--SQVQGFLCHCKRDFTGKSCQRRRNDQCDPN-PCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKK-DPGSLGRLKISSTKT---GLLKLQ 923 HSP 2 Score: 74.3294 bits (181), Expect = 8.955e-14 Identity = 74/264 (28.03%), Postives = 116/264 (43.94%), Query Frame = 0
Query: 503 CQCPE----DFRGDNCE--RRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRG-DSFMELDWKLLPHFDPTKIETVELEFSTTEPQ-GLIWYQDGVQKQLKDRN------FMALSISSGTPS---------FDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPS--------RLTHGRYVYGFRGCLHRLRIQGREIDFQ--RDPV 733
C CPE + +CE + + CD + PCQ GG C+ + C+C G G CE L + + F G DS +E+ +P D K +E+ F T Q GL+ Y V + F+ LS S+ F ++LG G+ L + I GK L R + G ++++ D GS++G L+ G+++I GG+ P+ R G + GF GC+ L+I + I Q R+P+
Sbjct: 507 CICPEGTSPNSANTSCEIMKIGSLCDPN-PCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIK---IP-TDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWI-GGVDPNDFPLMYKVRKNAGTSI-GFVGCIKELKINKKTISLQSMREPL 763 HSP 3 Score: 73.9442 bits (180), Expect = 1.001e-13 Identity = 82/329 (24.92%), Postives = 138/329 (41.95%), Query Frame = 0
Query: 16 CKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKP-ELANGLILYNAHDERGGKD--------FISFGLKNGKPEL---------RYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGT-----PSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCD--SCSLSPCENGGICQGSANDQGYRCICLPGFSGEHC-KREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQE--GSFCEDTTTIERPAFQGNSYLAIPRPK 315
C++ + + E ++L + Q S++E+ DS +++E+ F+ + GL+LY + FI N K + Y+LGS L + I G+W + R ++ L ++ + GS G L+L L++GG P + ++ + GF G L I + + Q M P ++ +G+ C+ SCS +PC+N G+C QG+ C C F+G+ C +R + C C N G C + F C C G E G++C D T + P Q +Y + PK
Sbjct: 553 CRSGLTGSRCEHDLSLTDMNTASFTGQD--SHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELK---INKKTISLQSM-REPLVIRRRGLVECEENSCSGNPCKNEGLCISQV--QGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCS-GSEYYGTYC-DLKTADTPNDQP-AYGSAKAPK 870
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000007498 (supercontig:LSalAtl2s:LSalAtl2s430:288057:377591:1 gene:EMLSAG00000007498 transcript:EMLSAT00000007498 description:"maker-LSalAtl2s430-augustus-gene-4.9") HSP 1 Score: 105.916 bits (263), Expect = 8.157e-24 Identity = 105/405 (25.93%), Postives = 163/405 (40.25%), Query Frame = 0
Query: 32 IVTHVIPHFNQAPLSYMELVTLPDSY-LNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFV-----DDDLPIRGS---APGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTA-ILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFG 426
I++ +P FN SY+ + DS L ++EL F+ +GLI YN G DFI+ L E Y+LG ++ + PI+ W+TV I+R+ + A + V +D L + S + G F L L LYLGG P+ + F F CI + + E+ L + + V +CD PC S+N+ F C G C+ F+C CP G G+ C+ T G S +PR ++ K A F A + R+ + GD+ ++ I +G L+ ++N GSG A I + I KW+ + L R ++ + SPG + LN +Y G
Sbjct: 346 IISKNVPSFNGT--SYLVYTGIGDSSPLWTEIELVFQSSAEDGLIFYNGQRNDGTDDFIAILLSRNYVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTFDLVSAYLPIRRSF--------FGCIQKLTINDVEINLVNSAISGVDVGSCD----HPCNTPS--SSSSNE----------FPQHPCGVH-----------GNCKPVLDGFKCDCPHGLTGNSCQKIT--------GESNNPLPR----FKKSKNALIFWAGNGRNF-------KKGIVGDYIALGIYNGRLQLKYNLGSGEASITYNWTRINDGKWHRIRLTRNELTASLRMDNGPIYTTMSPGKLKQLNANGEVYLG 694 HSP 2 Score: 91.6633 bits (226), Expect = 2.992e-19 Identity = 95/426 (22.30%), Postives = 161/426 (37.79%), Query Frame = 0
Query: 301 PAFQGNSYLA---IPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTM-FISG--QEPVKGE-----SPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDC--------SSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGR 707
P+F G SYL I + ++++ F+ A D ++ Y+ Q DG DF ++ + ++EF ++ G G ++RS PI WY V ++R + M I G ++P+K S G+ L + +Y GGV A + F GCI + ++L + ++ C SS+ ++ + PC G C+P D C CP G++C++ + + +P + W GRN +K G GD L Y +Q + ++ SG S +N INDGK R++L R S+++D + S G +L+ +G +++G S L H R
Sbjct: 352 PSFNGTSYLVYTGIGDSSPLWTEIELVFQSSA---EDGLIFYNGQRNDGTDDFIAILLSRNYVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTFDLVSAY-LPIRRSFFGCIQKLTINDVEINLVNSAISGVDVGSCDHPCNTPSSSSSNEFPQHPCGVHGNCKP--VLDGFKCDCPHGLTGNSCQKITGESNNPLPRFKKSK--------NALIFWAGNGRNFKK----------GIVGDYI-----------------------------ALGIYNGRLQ--------LKYNLGSGEASITYNWTR----------INDGKWHRIRLTRNELTASLRMDNGPIYTTMSPGKLKQLNANGEVYLG---XYSFLYHNR 703 HSP 3 Score: 51.2174 bits (121), Expect = 8.463e-7 Identity = 77/328 (23.48%), Postives = 133/328 (40.55%), Query Frame = 0
Query: 439 VGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRC---EGGTEFYKCFCPWGFGGRNC--------EKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEG----------SAELTASKVINDGKRL-RLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV---YGFRGCLHRLRIQGREIDFQRDPVSTGNLVPC 741
+G + GF GCI + K D SK ++GG Q G +CPED NC + +PCQ+ G+C E G + W NC + ++ N + F G S+ L + + P E +EL F ++ GLI+Y Q+ +F+A+ +S F ++LG+G S + + VI+ RL +++IR R+ +++ ++ S G +L+ + N+++GG P+ Y+ F GC+ +L I EI+ +S ++ C
Sbjct: 264 LGVETGFSGCIKRLEINNKVYDF----------------RSK------EHGGDSQFG----MDITECPED----NC--------VLIPCQHNGKCVPLESGHNIW-----WAI---NCGTLSISLRKGIISKNVPS---FNGTSY--LVYTGIGDSSPLWTE-IELVFQSSAEDGLIFYNG--QRNDGTDDFIAILLSRNYVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKM---PVSSRVSNGAFSQLTLEQNLYLGG--VPTFDLVSAYLPIRRSFFGCIQKLTINDVEINLVNSAISGVDVGSC 532
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000006314 (supercontig:LSalAtl2s:LSalAtl2s343:642:15163:-1 gene:EMLSAG00000006314 transcript:EMLSAT00000006314 description:"maker-LSalAtl2s343-augustus-gene-0.12") HSP 1 Score: 105.916 bits (263), Expect = 2.309e-23 Identity = 135/543 (24.86%), Postives = 208/543 (38.31%), Query Frame = 0
Query: 219 CDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYS-AQSEDGQGDFASVTIQDGHLEFRFNTG-SGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISG------------QEPVK------GESPGSTRGLNIRTPMYFGGVNKE-KYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEA-PCQNGGTCQPGKSKDQHTCQC-PEDFRGDNCE--------RRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMEL--DWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQID----GRDLAKGSSKGFTGRLSTDGNI-FIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ 722
D CS +PC NGG CQ D + C C PG+ G+ C+R + C C NGG C + D+ C CP G+ CE +T F S+ A P + + F A +S+++Y+ + G+ DF ++ + G +F + + IL P I +WY+V R + ++ I Q+ + GE+ G N PMY GG+ E + PG + F+GCI + D K ++++ I + ++ C + C GTC P S+ H C C E+ + +C+ Q D +P Q R Y W N E L + ++ +F L + LL D T I + + G++ YQ SSG +G +T+ +NDG LKL + ++ +D G D+ S+ F R NI I G K S GFRGC+ I
Sbjct: 3762 TDVCSPNPCFNGGSCQKGXRD-SFFCRCRPGYQGDRCERAVDSCLRNPCLNGGVCISQKPDYRCRCPDNFYGNRCELSTF----GFDELSFAAFPPLDSNTNDISITF---ATTKANSLLVYNHGEVSGGRSDFLALELVRGRPKFSWGAARTAVTILELPRKINNGRWYKVTATRNNRVASLSIEDCTESAEYCKLCQQDDSRCFSKDLGEAGTLVFGNN---PMYLGGLQSEIPFLERPGQLSSDDFVGCIKTLTINGNEKDFQKDALNTSRISSTCNFDNPCHKGNECGETGTCLPLWSR--HQCSCGTEEIKSIDCDASFAPFTLMEENQIDF-IPTQKFRRSRSLHSLYSHLNKW-----NDEPSLQNHEEYTLSALSLTFRSLQDNAVLLTSKDITGISKILI------INGILVYQ-----------------SSG-------VGLPEINMTSGHQVNDGLWHSLKLDSNDKVLNLFLDNAKVGYDIEFASAHKFLDR-----NIEKISLGFKDSD-------KGFRGCMTNFTIN 4243 HSP 2 Score: 104.375 bits (259), Expect = 7.254e-23 Identity = 84/301 (27.91%), Postives = 131/301 (43.52%), Query Frame = 0
Query: 474 CSSTESK-CDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAE-LTASKVINDGKRLRLKLIRKARKGSIQIDG--------RDLAKGSSKGFTGRLSTDGNIFIG------GGI--------KPSRLTHGRYVYGFRGCLHRLRIQGREIDFQRDPVSTGNLV-------PCSK 743
C +T++ C PC NGG+CQ G +D C+C ++GD CER C + PC NGG C Y+C CP F G CE ++ GF EL + P D + + + F+TT+ L+ Y G + +F+AL + G P F + + L + IN+G+ ++ R R S+ I+ + + S+ F+ L G + G GG+ +P +L+ +V GC+ L I G E DFQ+D ++T + PC K
Sbjct: 3757 CETTQTDVCSPNPCFNGGSCQKG-XRDSFFCRCRPGYQGDRCERAVDSC-LRNPCLNGGVCISQKPDYRCRCPDNFYGNRCEL-------STFGFD-----ELSFAAFPPLD-SNTNDISITFATTKANSLLVYNHG-EVSGGRSDFLALELVRGRPKFSWGAARTAVTILELPRKINNGRWYKVTATRNNRVASLSIEDCTESAEYCKLCQQDDSRCFSKDLGEAGTLVFGNNPMYLGGLQSEIPFLERPGQLSSDDFV----GCIKTLTINGNEKDFQKDALNTSRISSTCNFDNPCHK 4037 HSP 3 Score: 53.1434 bits (126), Expect = 3.469e-7 Identity = 94/437 (21.51%), Postives = 167/437 (38.22%), Query Frame = 0
Query: 61 DLELSFKPELANGLILYNAHDERGGK-DFISFGLKNGKPELRYNLGSETAILTAEKP--ITFGQWHTVKIARSRKNASLFVDD---------------------DLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSL--NNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSL-SPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRC-----QNTAKDFECFCPFGQEGSFCEDTTTIER----PAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAI----LRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRK 457
D+ ++F AN L++YN + GG+ DF++ L G+P+ + + TA+ E P I G+W+ V R+ + ASL ++D DL G A G P+YLGG S++ LE ++ F G CI + E L + + + +C+ +PC G C G CLP +S C E + C+ + DF F + S + + + P+ Q + ++ L L + FR +D+ VL +++ G + + I +G L ++ SG + + S + W+ + L+ K +F+ ++ + + F N EK +L +D GF GC+T+ ++
Sbjct: 3859 DISITFATTKANSLLVYNHGEVSGGRSDFLALELVRGRPKFSWG-AARTAVTILELPRKINNGRWYKVTATRNNRVASLSIEDCTESAEYCKLCQQDDSRCFSKDL---GEAGTLVFG---NNPMYLGGLQSEIPFLERPGQLSSDDFVG--------CIKTLTINGNEKDFQKDALNTSRISS--TCNFDNPCHKGNEC-------GETGTCLPLWSRHQCSCGTEEIKSIDCDASFAPFTLMEENQIDFIPTQKFRRSRSLHSLYSHLNKWNDEPSLQNHE-------EYTLSALSLTFR----SLQDNAVLLTSKDITG---ISKILIINGILVYQ---SSGVGLPEINMTSGHQVNDGLWHSLKLDSNDKVLNLFL--------DNAKVGYDIEFASAHKFLDRNIEKISLGFK-DSDKGFRGCMTNFTINQE 4245
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000003115 (supercontig:LSalAtl2s:LSalAtl2s174:638829:676917:-1 gene:EMLSAG00000003115 transcript:EMLSAT00000003115 description:"maker-LSalAtl2s174-augustus-gene-6.13") HSP 1 Score: 90.1225 bits (222), Expect = 9.194e-19 Identity = 129/624 (20.67%), Postives = 230/624 (36.86%), Query Frame = 0
Query: 59 NLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGS---ETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILR-SPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVN-KEKYTLAP----GVGTDLGFIGCITDIRFGRKTLDLSKVI--VDSANIRD-CSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQID 670
N D+ L FK ++GL+ Y + G D+++ G+K+G L LG+ +T I + +WH VK++R +L VDD R G F L T +++GG+ + L T N G C+ + ++L L EN D Y C + K + + + N + F + SF T+ + G+ ++ +FR + + ++L++ + D+ + I DGHL + GSG +R S P+ W+ V L ++G + I ++ ++PG L++ ++ GGV+ + Y P V GF+GC+ D+ ++D+ D +IR C +C + PC +GG C G ++ CD S G C G+ A+ +V F G + L +K T+ + + L F + E G+++ KD+ + +++ SG F +G + A + +ND + L R+ K + +D
Sbjct: 292 NDDISLFFKTRKSSGLLFYTGN----GNDYMNLGIKDGGVSLTVQLGTGKLDTQIKPSTVRFNDNKWHHVKVSRMSSEVTLTVDDLYNERWVTSGSFSVL-ASTKVFVGGSEA-TYTLPGTKAQNNLVG--------CLRSVIYTADSLILDLLGL---------------AENNNQLISVIGDVEYECQDIAAVPPLPFKSQED----SSSNMASIYMMQNNLGGIISFTTQESFLVSFTS-WKSTISGS----------------ISLKFRT-NELNGILLFNGGNP--HKDYFAFEILDGHLYVHLDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKID-EDSESFKTPGEANQLDLVGNLFVGGVDYMDPYMKFPPTLWTVTLKYGFVGCLRDLTINGASIDVVSYAHQQDVGSIRSSCHKMPDQCHDRPCNHGGKCSEGWNR--------------------FLCDCSRTSFTGATC-------------GY------------AAATVIFEGSHSISLSYK---DGTTTEADDIILRFRSMEQHGILFTTH--HDTSKDK--LDVTMESGRVRLSFQVGHSEKTIFAGQSLNDDIYHTILLQRRGTKIRVVVD 809 HSP 2 Score: 84.7297 bits (208), Expect = 4.384e-17 Identity = 171/720 (23.75%), Postives = 258/720 (35.83%), Query Frame = 0
Query: 58 LNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNG-KPELRYNL-----GSETAILT----AEKPITFGQWHTVKIARSRKNASLFVDDDL-------------------PIRGSAPGRFEGLDLMTPLYLGGTPS------QVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQG-DFASVTIQDGHLEFRFNTGSG---TAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQN-GGRCEGGTE--FYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKV-INDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGG------IK-PSRLTHGRYVYGFRGCLHRLRIQGREID 727
+N LEL F+ + L+LY + E +DF L +G K LRYN G+ I+T A P G H V++ ++ SL VDD P P +F + LY+GG P+ + L L F+G+ + F V QE VL+ +++ V+ CD PC N GIC LP SG C C G C+ + E G + +E P N + + F+ R + S +L+ G G D+ ++ I+DG + G+G T I S KW+ V ++R T+ + + + GS L T ++ GG ++ YTL PG +GC+ + + +L L + + N + S E CQ+ P K Q + + I M N GG T+ F F W ST G ++ L+F T E G++ + G +++ A I G +LG G ++ AS+ +NDG ++L R G I+ID D + G +L GN+F+GG +K P L YGF GCL L I G ID
Sbjct: 35 VNSTLELKFRTNQKDALLLYTDNIEH--RDFAQITLVSGSKVRLRYNWNGAQNGNRFNIITVNLGASSPP--GSVHRVRVLKNGAETSLIVDDRKESLLTPLVLTSTQSSASLPPSTIPVPYQFGDPVSNSFLYIGGLPTWYREKFDILTLPTVILEPRFRGEISDIKFKDARTKVFKIQE-VLAYKGVRASTVNACDHQX--PCLNDGIC-------------LPTDSGPICD-----CSKVDFIGLXCETKKQPLEATFRHGDYIXYDXQQRKVE-PILSTND------------DISLFFKTR----KSSGLLFYT----GNGNDYMNLGIKDGGVSLTVQLGTGKLDTQIKPSTVRFNDNKWHHVKVSRMSSEVTLTVDDLYNERWVTSGSFSVL-ASTKVFVGG-SEATYTL-PGTKAQNNLVGCLRSVIYTADSLILDLLGLAENNNQLISVIGDV--EYECQDIAAVPPLPFKSQE-------------DSSSNMASIYMMQNNLGGIISFTTQESFLVSFTSW--------------KSTISG-----------------------SISLKFRTNELNGILLFNGGNPH----KDYFAFEILDGHLYVHLDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKID-EDSESFKTPGEANQLDLVGNLFVGGVDYMDPYMKFPPTLWTVTLKYGFVGCLRDLTINGASID 648 HSP 3 Score: 64.6994 bits (156), Expect = 7.619e-11 Identity = 80/372 (21.51%), Postives = 143/372 (38.44%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGS-ETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQ------VQALEDTSLNNGFKG--KYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKG 408
S++ T S ++ + L F+ NG++L+N + KD+ +F + +G + +LGS I + P+ G WH V++ S+++ + +D+D PG LDL+ L++GG L +L GF G + L + I Q+ V +++S D C PC +GG C N R +C C R FTGA C + E + +I G + A + RFR+++ +L++ D D VT++ G + F G + + + + ++ + L R+ + + EP+ G
Sbjct: 493 SFLVSFTSWKSTISGSISLKFRTNELNGILLFNGGNPH--KDYFAFEILDGHLYVHLDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKIDEDSE-SFKTPGEANQLDLVGNLFVGGVDYMDPYMKFPPTLWTVTLKYGFVGCLRDLTINGASIDVVSYAHQQDV---GSIRSSCHKMPDQCHDRPCNHGGKCSEGWN----RFLC-------DCSRTS---FTGAT---------------CGYAAATVIFEGSHSISLSYKDGTTTEAD----------DIILRFRSMEQHG--ILFTTH-HDTSKDKLDVTMESGRVRLSFQVGHSEKTIFAGQSLNDDIYHTILLQRRGTKIRVVVDDDEPIDG 816
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000000936 (supercontig:LSalAtl2s:LSalAtl2s1165:50208:68601:-1 gene:EMLSAG00000000936 transcript:EMLSAT00000000936 description:"maker-LSalAtl2s1165-augustus-gene-0.27") HSP 1 Score: 87.0409 bits (214), Expect = 1.254e-17 Identity = 150/568 (26.41%), Postives = 211/568 (37.15%), Query Frame = 0
Query: 56 SYLNLDLELSFKPE---LANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITF---GQWHTVKIARSRKNASLFVD-----------DDLP---------IRGSAPGRFEGLDLMTPLYLGGTPS-QVQALE----DTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIP---RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPE------PIYAK--KWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTP-----MYFGGVNKEKYTLAPGVGTDLGFI----GCITDIRFGRKTLDLSKVI--------VDSANI-RDCSSTESKCDEAPCQNGGTCQPGKSKD---QHTCQCPEDFR----GDNCERRATQCDISMPCQNGGRC--EGGTEFYKCFCPWGFGGRNCE 554
S+L+++ L+ KP+ L NG I+ +E+ DF+S L P L + GS T L E T G WH + I R+ + +D D P ++G P E L++ TPL +GG + Q L T L F G CI + ++ L+ L C P E C N G RC GEH G C + K C C G G FC T F SY+ P ++++ FR TR+ S+ ++ + IQ+ L FR+N S LR+ E IY +W+ V + R A + + G E + G + T +Y GG K +YT GV T + GC+ DIR K L L + + N+ R+C S PC N +P D ++ C CP R G +C R +C + PCQN G+C E Y C CP GF G NCE
Sbjct: 2288 SHLSIEF-LTRKPDGVLLYNGPIVPPEPEEQLISDFLSLELVKAHPRLLVDFGSGTLELRIEPTKTRLDDGSWHRIDIFWDREQVRMVLDFCRSSVVVEHEDGSPTEFDDSSCQVKGDVPPFNEYLNVNTPLQIGGMAAXQFDPLHYKWSHTPLGKPFDG--------CIRNIIHNSKLYDLAEPGLFRYSKPGC-----IPVEKA--CND--NYPGSRC-------GEH---------------GHCVGSLKHPTCKCNPGWTGQFCSHETMPS--TFDPQSYVKYALSFEPDSFATEIQLRFR-----TREEHGELFRVSDQHNREYGILEIQESKLRFRYNLNS----LRTEEKEIWLSSIYVDDGQWHTVKVARYGSAAILTLDGGESRRYNDSFIFDGHQLMTVDKQEGVYAGG--KAEYT---GVRTFEVYSDYAKGCMDDIRLEGKXLPLPPALNGTQWGQATMARNLQRNCPSNN------PCANVICPKPFVCVDLWMEYECSCPRGQRVTPDGKSCVDR-NEC-LDNPCQNNGQCVNRDTAERYICHCPSGFSGSNCE 2791 HSP 2 Score: 77.0258 bits (188), Expect = 1.599e-14 Identity = 122/534 (22.85%), Postives = 190/534 (35.58%), Query Frame = 0
Query: 244 CICLPGFSGEHCKREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSA------QSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAK----KWYE--------------------VALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKY--TLAPGVGTDLG--FIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIET-VELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAE----LTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGN----------IFIGGGIKPSRL-THGRYVYGFRGCLHRLRIQGREI 726
C+C G + E C T C NGGRC C CP G EG C+ TT F G + P P + Q ++ F D V+LY+ E DF S+ + H + GSGT LR EP + W+ V + + + T F VKG+ P LN+ TP+ GG+ ++ T LG F GCI +I K DL++ + + C E C++ PG C G C G + C C G+ G+ C + + F S++ K F+P T ++L F T E G ++ V Q +R + L I F +NL E +S ++DG+ +K+ R + +DG G S+ + DG+ ++ GG + + + T Y +GC+ +R++G+ +
Sbjct: 2220 CVC-----GARNFEKAESCLTHVCYNGGRCLEGRWGVRCQCPRGYEGPRCQQTTR----TFWGKGWAWYP-PLELCEQSHLSIEF-LTRKPDGVLLYNGPIVPPEPEEQLISDFLSLELVKAHPRLLVDFGSGTLELRI-EPTKTRLDDGSWHRIDIFWDREQVRMVLDFCRSSVVVEHEDGSPTEFDDSSCQVKGDVPPFNEYLNVNTPLQIGGMAAXQFDPLHYKWSHTPLGKPFDGCIRNIIHNSKLYDLAEPGLFRYSKPGCIPVEKACNDN--------YPGSR-----------------------------CGEHGHCVGSLKHPTCKCNPGWTGQFCS-----HETMPSTFDPQSYV----KYALSFEPDSFATEIQLRFRTREEHGELFR---VSDQ-HNREYGILEIQESKLRFRYNLNSLRTEEKEIWLSSIYVDDGQWHTVKVARYGSAAILTLDG-----GESRRYNDSFIFDGHQLMTVDKQEGVYAGGKAEYTGVRTFEVYSDYAKGCMDDIRLEGKXL 2686 HSP 3 Score: 55.8398 bits (133), Expect = 4.430e-8 Identity = 76/280 (27.14%), Postives = 112/280 (40.00%), Query Frame = 0
Query: 44 PLSYME--LVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGS----ETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTP-----LYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSP---------ATLKSQGVDTCDS---CSLSPCENGGICQGSANDQGYRCICLPGF----SGEHCKREGEMCFTGAC-NGGRCQN--TAKDFECFCPFGQEGSFCE 293
P SY++ L PDS+ +++L F+ +G L+ D+ +++ ++ K RYNL S E I + + GQWHTVK+AR A L +D R + F+G LMT +Y GG ++ KG C+ + + + L P AT+ C S C+ C +C + Y C C G G+ C E C C N G+C N TA+ + C CP G GS CE
Sbjct: 2526 PQSYVKYALSFEPDSFAT-EIQLRFRTREEHGE-LFRVSDQHN-REYGILEIQESKLRFRYNLNSLRTEEKEIWLSSIYVDDGQWHTVKVARYGSAAILTLDGGESRRYNDSFIFDGHQLMTVDKQEGVYAGGKAEYTGVRTFEVYSDYAKG--------CMDDIRLEGKXLPLPPALNGTQWGQATMARNLQRNCPSNNPCANVICPKPFVCVDLWME--YECSCPRGQRVTPDGKSCVDRNE-CLDNPCQNNGQCVNRDTAERYICHCPSGFSGSNCE 2791
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000005353 (supercontig:LSalAtl2s:LSalAtl2s289:184980:192687:-1 gene:EMLSAG00000005353 transcript:EMLSAT00000005353 description:"maker-LSalAtl2s289-snap-gene-2.32") HSP 1 Score: 87.0409 bits (214), Expect = 1.333e-17 Identity = 82/356 (23.03%), Postives = 138/356 (38.76%), Query Frame = 0
Query: 218 TCDS----CSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACN------------------GGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAIL---RSPEPIYAKKWYEVAL--------------------------NRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGV--NKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQ 520
TCD C SPC NG C ND Y CIC P + G++C+ T +C G CQN ++++ FC+ ++ +F +Y+A K L + F A +R ++ Y+ + +G+ DF S++ +D +L+F F+ G L R E +++V L + KLK I + ++ P+ GG+ K++ + F GCI ++ + LD++ + D+ ++ C + C PC+NGG C G Q C+C D+ G NC R ++
Sbjct: 1116 TCDVPINMCYSSPCLNGAKCVSKEND--YSCICAPNYVGKNCELS---LLTSSCTDNQDLCKLPSRCVDLQKGGVTCQNCSRNY-------YHDQFCQ----LKSRSFSPGTYIAFNSIK-ARNSLNLTLEF-ASQSRRGLLFYNGRL-NGEHDFMSISYEDAYLKFSFSLGDKNQELIVNRVKEGFSDGHFHQVELIIENEHITLSYDFCDKKLSLNHQSRLDSKLKCANRTIFQHSECNSYLGNCRKSFDLSGPLLLGGIPDGKDEKRIKTR-----SFSGCIKNVAIDQFELDMNSYVHDNGSVSGCPEKRNFCMSNPCKNGGICSEGWGSYQ--CKCHSDWDGKNCNHRVSK 1445
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000003184 (supercontig:LSalAtl2s:LSalAtl2s176:575372:647076:1 gene:EMLSAG00000003184 transcript:EMLSAT00000003184 description:"augustus_masked-LSalAtl2s176-processed-gene-6.1") HSP 1 Score: 83.1889 bits (204), Expect = 1.299e-16 Identity = 70/282 (24.82%), Postives = 118/282 (41.84%), Query Frame = 0
Query: 485 PCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNAST--------------SVGFRGDSFMELDWKLLPHFDPTKIE------------TVELEFSTTEPQGLIWY------QDGVQKQLKDRNFMALSISSGTPSFDFNLGE-GSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGG-----GIKPSRLTHGRYVYGFRGCLHRLRIQGREIDF 728
PC N G C + +TC+C F G NC + C ++ CQNGG CE G + Y C CP FGG+ CE + PM A + F+ K P + + E VE+E +P+ I Q+GV D +A+ + G +++G + + + ++++DGK R++L+ + ++++DG ++G L ++FI G G K + H R F+GC+ + + + DF
Sbjct: 415 PCMNAGKCVDHFT--HYTCECKAGFSGANCTKNIDDC-LNHMCQNGGTCEDGIDSYTCKCPQEFGGKFCE-IEPMVAQLYPQTSPCQQHDCKHGICFQPPGSNNYICKCAPGYSGKRCEYLTSLSFIHNTSYVEMEALNVKPKANITIIFATEQQNGVLLYTGDNQHLAVELFRGRIRVSYDVGNYPVSTMFSYELLSDGKYHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGVPEDVGHKSLKQWHLRNSTSFKGCIKEVYVNDKLSDF 692 HSP 2 Score: 62.003 bits (149), Expect = 5.022e-10 Identity = 56/230 (24.35%), Postives = 102/230 (44.35%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGS-ETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQV--QALEDTSLNNG--FKGKYLVLVFCCIGRFVVGTQ-EMVLSPATLKSQGVDTC---------DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGE 260
SY+E+ L + ++ + F E NG++LY G ++ L G+ + Y++G+ + + + + ++ G++H V++ +KN +L VD + G E L++ L++ G P V ++L+ L N FKG CI V + L A ++ + C D C C++ G C+ + Y CIC P F+G C+ G+
Sbjct: 541 SYVEMEAL-NVKPKANITIIFATEQQNGVLLYT-----GDNQHLAVELFRGRIRVSYDVGNYPVSTMFSYELLSDGKYHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGVPEDVGHKSLKQWHLRNSTSFKG--------CIKEVYVNDKLSDFLQAAKVRHKVTPGCSLYQDEQPTDPCVDHKCKHEGECRSKNDGTSYECICKPHFTGHFCQIRGK 756 HSP 3 Score: 57.7658 bits (138), Expect = 8.449e-9 Identity = 76/353 (21.53%), Postives = 127/353 (35.98%), Query Frame = 0
Query: 224 LSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIER--------------------------------PAFQGN--SYLAIPRPKHILRQLKM-AFRFR---------AVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGS-GTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKE---KYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRD-----CSSTESK-----CDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERR 517
+PC N G C Y C C GFSG +C + + C C NGG C++ + C CP G FCE + + P + G YL H ++M A + A + ++ V+LY+ G +V + G + ++ G+ + + S E + K++ V L K T+ + G + G+ L IR ++ GV ++ K + F GCI ++ K D + +A +R CS + + C + C++ G C+ + C C F G C+ R
Sbjct: 413 FNPCMNAGKCVDHFTH--YTCECKAGFSGANCTKNIDDCLNHMCQNGGTCEDGIDSYTCKCPQEFGGKFCEIEPMVAQLYPQTSPCQQHDCKHGICFQPPGSNNYICKCAPGYSGKRCEYLTSLSFIHNTSYVEMEALNVKPKANITIIFATEQQNGVLLYT-----GDNQHLAVELFRGRIRVSYDVGNYPVSTMFSYELLSDGKYHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGVPEDVGHKSLKQWHLRNSTSFKGCIKEVYVNDKLSDF----LQAAKVRHKVTPGCSLYQDEQPTDPCVDHKCKHEGECRSKNDGTSYECICKPHFTGHFCQIR 754 HSP 4 Score: 51.6026 bits (122), Expect = 7.440e-7 Identity = 32/115 (27.83%), Postives = 43/115 (37.39%), Query Frame = 0
Query: 211 LKSQGVDTCDSCSLSPCENGGICQGSAN--------------------DQGYRCICLPGFSGEHCKREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQ 304
+ S + CD C SPC+NG +C N D RCIC GF G C+ + C C N C + ++EC C G G +CE + F
Sbjct: 300 IPSNILAKCDLCHTSPCQNGAMCNXLPNRDYQCICAPGXYGKNCDSVIDAXLRCICPSGFEGVRCETNTDDCSKHICENNSTCVDKIGEYECTCSLGFSGKYCEKKIPFCKKEFN 414
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000011824 (supercontig:LSalAtl2s:LSalAtl2s829:38411:43233:1 gene:EMLSAG00000011824 transcript:EMLSAT00000011824 description:"maker-LSalAtl2s829-augustus-gene-0.4") HSP 1 Score: 79.7221 bits (195), Expect = 1.565e-15 Identity = 42/79 (53.16%), Postives = 48/79 (60.76%), Query Frame = 0
Query: 479 SKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVL 557
++C PC NGGTCQ GK +TC CP F GD+CE+RA C S PCQN G C G + Y C CP GF G NCE L
Sbjct: 314 NECSHQPCLNGGTCQ-GKGSSNYTCVCPVGFHGDHCEKRAQXCHDS-PCQNNGACTNGPQGYICGCPHGFSGINCEIKL 390 HSP 2 Score: 68.9366 bits (167), Expect = 2.931e-12 Identity = 35/82 (42.68%), Postives = 42/82 (51.22%), Query Frame = 0
Query: 474 CSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEK 555
C C ++PCQN G C G + C CP F G NCE + C PC N G+C EFY+C CP GF G NCE+
Sbjct: 348 CEKRAQXCHDSPCQNNGACTNGPQG--YICGCPHGFSGINCEIKLDACRADAPCLNNGKCIPVGEFYRCECPLGFTGPNCEE 427 HSP 3 Score: 64.6994 bits (156), Expect = 7.081e-11 Identity = 35/75 (46.67%), Postives = 42/75 (56.00%), Query Frame = 0
Query: 482 DEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEG-GTEFYKCFCPWGFGGRNCEK 555
+ PC+NG TC + +TC CP F G NCE R T PC NGG C+G G+ Y C CP GF G +CEK
Sbjct: 277 NHEPCRNGATCY-NTGQGSYTCNCPPGFTGINCETRITNECSHQPCLNGGTCQGKGSSNYTCVCPVGFHGDHCEK 350 HSP 4 Score: 60.4622 bits (145), Expect = 1.296e-9 Identity = 31/75 (41.33%), Postives = 40/75 (53.33%), Query Frame = 0
Query: 220 DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCE 293
+ CS PC NGG CQG + Y C+C GF G+HC++ + C C N G C N + + C CP G G CE
Sbjct: 314 NECSHQPCLNGGTCQGKGSSN-YTCVCPVGFHGDHCEKRAQXCHDSPCQNNGACTNGPQGYICGCPHGFSGINCE 387 HSP 5 Score: 55.4546 bits (132), Expect = 4.812e-8 Identity = 31/72 (43.06%), Postives = 38/72 (52.78%), Query Frame = 0
Query: 483 EAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCE 554
+APC N G C P + + C+CP F G NCE C S PC+N G C +KC+CP GF G CE
Sbjct: 396 DAPCLNNGKCIP--VGEFYRCECPLGFTGPNCEENVDDCS-SSPCRNSGTCIDLISDFKCYCPPGFTGLQCE 464 HSP 6 Score: 53.5286 bits (127), Expect = 1.845e-7 Identity = 30/85 (35.29%), Postives = 42/85 (49.41%), Query Frame = 0
Query: 474 CSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLP 558
C + C PC NGGTC+ G + C CP F G +C +C + PC N G C + ++C CP G+ G+ C +LP
Sbjct: 463 CEINVNDCRRHPCFNGGTCRDGX--NDFICTCPHGFTGRDCSIEINEC-LPNPCMNDGFCVDRMDDFRCHCPRGYSGKTC-NILP 543 HSP 7 Score: 53.5286 bits (127), Expect = 2.097e-7 Identity = 40/128 (31.25%), Postives = 53/128 (41.41%), Query Frame = 0
Query: 220 DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTR-DSVVLYSAQSED 345
D CS SPC N G C +D ++C C PGF+G C+ C C NGG C++ DF C CP G G C P N + R + + + D VL S +S+D
Sbjct: 430 DDCSSSPCRNSGTCIDLISD--FKCYCPPGFTGLQCEINVNDCRRHPCFNGGTCRDGXNDFICTCPHGFTGRDCSIEINECLPNPCMNDGFCVDRMDDFRCHCPRGYSGKTCNILPDGTVLASPRSQD 555 HSP 8 Score: 53.1434 bits (126), Expect = 2.133e-7 Identity = 32/81 (39.51%), Postives = 37/81 (45.68%), Query Frame = 0
Query: 473 DCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNC 553
+C C +PC+N GTC S C CP F G CE C PC NGG C G + C CP GF GR+C
Sbjct: 424 NCEENVDDCSSSPCRNSGTCIDLIS--DFKCYCPPGFTGLQCEINVNDCR-RHPCFNGGTCRDGXNDFICTCPHGFTGRDC 501
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Match: EMLSAG00000012352 (supercontig:LSalAtl2s:LSalAtl2s903:176857:182009:1 gene:EMLSAG00000012352 transcript:EMLSAT00000012352 description:"maker-LSalAtl2s903-augustus-gene-1.45") HSP 1 Score: 73.1738 bits (178), Expect = 6.737e-15 Identity = 53/155 (34.19%), Postives = 81/155 (52.26%), Query Frame = 0
Query: 593 LEFSTTEPQGL-IWYQDGVQKQLKDRNFMALSISSGTPSFDFNLG--EGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIK-PSRLTHGRYVYGFRGCLHRLRIQGREIDFQRDPVSTGNLVPCSK 743
+ T E GL IW G LK +F+A++I G P + LG + + + + K IND K RLK+IR+ R G IQ+D +G S L+T+G +++GG P L H +Y GF+GC+ +++I + +D R NL CS+
Sbjct: 54 VHLRTIEKNGLLIWINQGTS--LKG-DFLAVAIVDGYPELSYKLGKKQNTLRIKSKKRINDDKWHRLKVIRRRRTGLIQVDKLKPIRGKSGTGASILNTNGKVWLGGKEPLPPGLPH-QYYKGFKGCIRKVKIFRKRLDLLRHG-DNSNLKLCSR 203 HSP 2 Score: 67.781 bits (164), Expect = 4.255e-13 Identity = 46/160 (28.75%), Postives = 80/160 (50.00%), Query Frame = 0
Query: 322 KMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILR--SPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDL--GFIGCITDIRFGRKTLDLSKVIVDSANIRDCSST 477
K R ++ ++ ++++ Q +GDF +V I DG+ E + G LR S + I KW+ + + R+ + G + + +P++G+S LN ++ GG K L PG+ GF GCI ++ RK LDL + D++N++ CS T
Sbjct: 51 KFTVHLRTIE-KNGLLIWINQGTSLKGDFLAVAIVDGYPELSYKLGKKQNTLRIKSKKRINDDKWHRLKVIRRRRTGLIQVDKLKPIRGKSGTGASILNTNGKVWLGG----KEPLPPGLPHQYYKGFKGCIRKVKIFRKRLDLLRH-GDNSNLKLCSRT 204 HSP 3 Score: 51.6026 bits (122), Expect = 1.491e-7 Identity = 30/96 (31.25%), Postives = 50/96 (52.08%), Query Frame = 0
Query: 72 NGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSE--TAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGG 165
NGL+++ DF++ + +G PEL Y LG + T + ++K I +WH +K+ R R+ + VD PIRG + L+ ++LGG
Sbjct: 62 NGLLIWINQGTSLKGDFLAVAIVDGYPELSYKLGKKQNTLRIKSKKRINDDKWHRLKVIRRRRTGLIQVDKLKPIRGKSGTGASILNTNGKVWLGG 157
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|317373536|sp|P98160.4|PGBM_HUMAN (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; AltName: Full=Perlecan; Short=PLC; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor) HSP 1 Score: 431.024 bits (1107), Expect = 7.652e-130 Identity = 258/783 (32.95%), Postives = 393/783 (50.19%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTH-VIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGK---------DFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEM-CFTGACN-GGRCQNT--AKDFECFCPFGQEGSFCEDTTTIERPAFQG-NSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTS-----------------VGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQ--KQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQG--------REIDFQRDPVSTGNLVPC 741
L +P V DAG Y+C A N +V+ L V V+P+F Q P S++ L T+ D+Y +++++F+P+ A+G++LYN G DFISFGL G+PE R++ GS A + P+ G +HTV + RS SL V D P+ G++ G+F+GLDL LYLGG P A+ L++GF G C+ + +E+V L + G+ C +C PC+NGG C S + Y C+C GF+G C+ + C AC C N + + C C G+ G CE+ T+ P+ G SYLA+P + +L++ F+ + D V+L+S DF S+ + GHLEFR+ GSG A+LRS EP+ +W+ V+ R K G++ ++G PV SPG ++GLN+ T +Y GGV + L+P F GC+ ++ K LDL+ + S I C + S C+ PCQ+G TC P + + C C + F+GD CE C + PC +GG C+G +C C GF G C++ + S F D F+ + P ET+ELE T+ GL+ +Q GV+ + + ++F++L + G F + LG G A L + INDG+ R+ +R+ R+GSIQ+DG +L G S G ++ G+++IGG + LT GR+ G GC+ L + + +D Q + N PC
Sbjct: 3626 LMLPSVRPQDAGTYVCTATNRQGKVKAFAHLQVPERVVPYFTQTPYSFLPLPTIKDAYRKFEIKITFRPDSADGMLLYNGQKRVPGSPTNLANRQPDFISFGLVGGRPEFRFDAGSGMATIRHPTPLALGHFHTVTLLRSLTQGSLIVGDLAPVNGTSQGKFQGLDLNEELYLGGYP-DYGAIPKAGLSSGFIG--------CVRELRIQGEEIVFHDLNLTAHGISHCPTCRDRPCQNGGQCHDSESSS-YVCVCPAGFTGSRCEHSQALHCHPEACGPDATCVNRPDGRGYTCRCHLGRSGLRCEEGVTVTTPSLSGAGSYLALPALTNTHHELRLDVEFKPL-APDGVLLFSGGKSGPVEDFVSLAMVGGHLEFRYELGSGLAVLRSAEPLALGRWHRVSAERLNKDGSLRVNGGRPVLRSSPGKSQGLNLHTLLYLGGV-EPSVPLSPATNMSAHFRGCVGEVSVNGKRLDLTYSFLGSQGIGQCYDS-SPCERQPCQHGATCMPA-GEYEFQCLCRDGFKGDLCEHEENPCQLREPCLHGGTCQG----TRCLCLPGFSGPRCQQGSGHGIAESDWHLEGSGGNDAPGQYGAYFHDDGFLAFPGHVFSRSLPEVPETIELEVRTSTASGLLLWQ-GVEVGEAGQGKDFISLGLQDGHLVFRYQLGSGEARLVSEDPINDGEWHRVTALREGRRGSIQVDGEELVSGRSPGPNVAVNAKGSVYIGGAPDVATLTGGRFSSGITGCVKNLVLHSARPGAPPPQPLDLQHRAQAGANTRPC 4389
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|1172451|sp|Q05793.1|PGBM_MOUSE (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor) HSP 1 Score: 422.55 bits (1085), Expect = 7.232e-127 Identity = 250/780 (32.05%), Postives = 386/780 (49.49%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTH-VIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGK------DFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSG------EHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQG-NSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTS-----------------VGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQG--------REIDFQRDPVSTGNLVPC 741
L +P V +DAG Y+C A N +V+ L V VIP+F Q P S++ L T+ D+Y +++++F+P+ A+G++LYN DFISFGL G+PE R++ GS A + P+ GQ+HTV + RS SL V + P+ G++ G+F+GLDL LYLGG P A+ L++GF G C+ + +E+V L + G+ C +C PC+NGG CQ S + Y C+C GF+ + C + C + + + C C G+ G CE+ T+ P+ G SYLA+P + +L++ F+ ++ + ++L+S DF S+ + GHLEFR+ GSG A+LRS EP+ +W+ V+ R K G++ + G PV SPG ++GLN+ T +Y GGV + L+P F GC+ ++ K LDL+ + S + C + S C+ PC+NG TC P + + C C + F+GD CE C + PC NGG C G +C C GF G C++ S F + F+ L P ET+E E T+ GL+ +Q V++ + ++F++L + G F + LG G A L + INDG+ R+ +R+ ++GSIQ+DG DL G S G ++T I+IGG + LT G++ G GC+ L + + +D Q + N PC
Sbjct: 2947 LMLPSVRPEDAGTYVCTATNRQGKVKAFAYLQVPERVIPYFTQTPYSFLPLPTIKDAYRKFEIKITFRPDSADGMLLYNGQKRSPTNLANRQPDFISFGLVGGRPEFRFDAGSGMATIRHPTPLALGQFHTVTLLRSLTQGSLIVGNLAPVNGTSQGKFQGLDLNEELYLGGYPD-YGAIPKAGLSSGFVG--------CVRELRIQGEEIVFHDVNLTTHGISHCPTCQDRPCQNGGQCQDSESSS-YTCVCPAGFTAAAVNIRKPCTATPSLWADATCVN---RPDGRGYTCRCHLGRSGVRCEEGVTVTTPSMSGAGSYLALPALTNTHHELRLDVEFKPLEP-NGILLFSGGKSGPVEDFVSLAMVGGHLEFRYELGSGLAVLRSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNLHTLLYLGGV-EPSVQLSPATNMSAHFHGCVGEVSVNGKRLDLTYSFLGSQGVGQCYDS-SPCERQPCRNGATCMPA-GEYEFQCLCQDGFKGDLCEHEENPCQLHEPCLNGGTCRGA----RCLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGLPGNSFSRSLPEVPETIEFEVRTSTADGLLLWQGVVREASRSKDFISLGLQDGHLVFSYQLGSGEARLVSGDPINDGEWHRITALREGQRGSIQVDGEDLVTGRSPGPNVAVNTKDIIYIGGAPDVATLTRGKFSSGITGCIKNLVLHTARPGAPPPQPLDLQHRAQAGANTRPC 3705 HSP 2 Score: 195.667 bits (496), Expect = 2.559e-50 Identity = 152/505 (30.10%), Postives = 227/505 (44.95%), Query Frame = 0
Query: 6 VTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTC---DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKRE------GEMCFTGAC------------NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIP--RPKHILRQLKMAFRFRA-VDTRDSVVLYSAQSEDG--QGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGR--------KTLDLSKVIVDSANIRDCSS 476
V D Y C+ + + V + VT P + A SY+ L L +++ L L++ FKP NG++L++ +DF+S + G E RY LGS A+L + +P+ G+WH V R K+ SL VD P+ S+PG+ +GL+L T LYLGG VQ T+++ F G C+G V + + L+ + L SQGV C C PC NG C A + ++C+C GF G+ C+ E E C G +G RCQ A + EGS D F N +L +P L ++ F T D ++L+ + DF S+ +QDGHL F + GSG A L S +PI +W+ + R+ + G++ + G++ V G SPG +N + +Y GG + TL G + G GCI ++ + LDL AN R C S
Sbjct: 3217 VNRPDGRGYTCRCHLGRSGVRCEEGVTVT--TPSMSGA-GSYLALPALTNTHHELRLDVEFKPLEPNGILLFSGGKSGPVEDFVSLAMVGGHLEFRYELGSGLAVLRSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNLHTLLYLGGVEPSVQLSPATNMSAHFHG--------CVGEVSVNGKRLDLTYSFLGSQGVGQCYDSSPCERQPCRNGATCM-PAGEYEFQCLCQDGFKGDLCEHEENPCQLHEPCLNGGTCRGARCLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGLPGNSFSRSLPEVPETIEFEVRTSTADGLLLWQGVVREASRSKDFISLGLQDGHLVFSYQLGSGEARLVSGDPINDGEWHRITALREGQRGSIQVDGEDLVTGRSPGPNVAVNTKDIIYIGGA-PDVATLTRGKFSS-GITGCIKNLVLHTARPGAPPPQPLDLQHRAQAGANTRPCPS 3707
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|218563482|sp|A2ASQ1.1|AGRIN_MOUSE (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22) HSP 1 Score: 277.715 bits (709), Expect = 1.157e-77 Identity = 227/725 (31.31%), Postives = 343/725 (47.31%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQA--LEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSP---ATLKSQGVDTC--DSCSLSPCENGGICQGSANDQG-YRCICLPGFSGEHCKREGEMCFTGACNG-GRCQNTAK-DFECFCPFGQEGSFCEDTTTIE----RP---AFQGNSYLAIPRPKHILRQL--KMAFRFRAVDTRDS-VVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTR----GLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRF-GRKTLDLSKVI--VDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGG--GIKPSRLTHGRYVYGFRGCLHRLRIQGREIDFQRDPVSTGNLVPC 741
S++ TL +Y L L L F+ GL+LYN + GKDF++ L +G + R++ GS A+LT+ P+ G+WH ++++R + +L VD + P+ G +P +GL+L T LY+GG P + A L+ TS+ G KG CI + Q++ LS A ++S GV C CS +PC G +CQ A + G + C C PG G C E C C+G C ++ +C CP G+ GSFCE T +E RP F G SYL + R L KMA + S ++LY+ Q DG+GDF S+ + + HLEFR++ G G AI+RS EPI W V L R + G + + V GESP S + LN++ P+Y GG + LA G GF G I + G + L V+ VD A T++ + PC NGG+C P ++ + C CP F G +CE+ + + G E F GR + L + + + LD + L + EL T QGL+ + + K + ++MAL+I G ++LG L ++ +N + LR++ R+ R+GS+Q+ GSS +L TDG +++GG + + Y GF GCL + + R++ D V+ L PC
Sbjct: 1263 SFLAFPTL-RAYHTLRLALEFRALETEGLLLYNGNAR--GKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKG--------CIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQ--ALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCE--TVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGA-PDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAV--DNPCLNGGSCIPREAT--YECLCPGGFSGLHCEKGIVEKSV-----------GDLETL------AFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLW---IGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPC 1947 HSP 2 Score: 214.157 bits (544), Expect = 1.990e-56 Identity = 158/505 (31.29%), Postives = 250/505 (49.50%), Query Frame = 0
Query: 256 KREGEMCFTGAC-NGGRCQN--TAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIP--RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKY-TLAPGVGTDLGFIGCITDIRFGRKTLDLS---KVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCE----GGTEFYKCFCPWGFGGRNCEKVLPMNASTS--VGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQI-DGRDL---AKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVY-GFRGCLHRLRIQGREIDFQRDPVSTGNLVP 740
+R + C + C +GG CQ+ + K F C C G+ G+ CE PAF+G+S+LA P R H LR +A FRA++T + ++LY+ + DF ++ + DGH++FRF+TGSG A+L S P+ +W+ + L+R + GT+ + G+ PV GESP T GLN+ T +Y GG+ +E+ T+ +G GCI + + L+LS + +V S+ + +C + C PC G CQ CQCP G C C + PC C GG KC CP G G CE VL S F G S++EL D + +E+ F P GL+ Y QK +F++L++ + F ++LG+G+A + + + I G +R+ L R RKG++Q+ DG + + S K L+ +++GG S+L G V GF G + + ++G ++ Q + ++ P
Sbjct: 1209 QRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLR---LALEFRALET-EGLLLYNGNARGK--DFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECG--DHPCSPNPCHGGALCQ-ALEAGVFLCQCPPGRFGPTCADEKNPCQPN-PCHGSAPCHVLSRGGA---KCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNG--QKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAP 1698
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|399021|sp|P25304.2|AGRIN_RAT (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22) HSP 1 Score: 273.092 bits (697), Expect = 3.651e-76 Identity = 221/723 (30.57%), Postives = 342/723 (47.30%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQA--LEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSP---ATLKSQGVDTC--DSCSLSPCENGGICQGSANDQG-YRCICLPGFSGEHCKREGEMCFTGACNGGR-CQN-TAKDFECFCPFGQEGSFCEDT--TTIERP---AFQGNSYLAIPRPKHILRQL--KMAFRFRAVDTRDS-VVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTR----GLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRF-GRKTLDLSKVI--VDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGG--GIKPSRLTHGRYVYGFRGCLHRLRIQGREIDFQRDPVSTGNLVPC 741
S++ TL +Y L L L F+ GL+LYN + GKDF++ L +G+ + R++ GS A+LT+ P+ G+WH ++++R + +L VD + P+ G +P +GL+L T LY+GG P + A L+ TS+ G KG CI + Q++ LS A ++S GV C C +PC G +CQ A + G + C C PG G C E C C+G C+ ++ +C CP G+ G+FC+ T RP F G SYL + R L KMA + S ++LY+ Q DG+GDF S+ + + HLEF ++ G G A++RS EPI W V L R + G + + V GESP S + LN++ P+Y GG + LA G GF G I + G + L V+ VD + D T++ + PC NGG+C P ++ + C CP F G +CE+ + + G E F GR + L + + + LD + L + EL T QGL+ + + K + ++MAL+I G ++LG L ++ +N + LR++ R+ R+GS+Q+ GSS +L TDG +++GG + + Y GF GCL + + R++ D V+ L PC
Sbjct: 1272 SFLAFPTL-RAYHTLRLALEFRALETEGLLLYNGNAR--GKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKG--------CIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQ--ALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGA-PDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGN--PCLNGGSCVPREAT--YECLCPGGFSGLHCEKGLVEKSV-----------GDLETL------AFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLW---IGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPC 1956 HSP 2 Score: 206.838 bits (525), Expect = 4.107e-54 Identity = 154/492 (31.30%), Postives = 242/492 (49.19%), Query Frame = 0
Query: 268 NGGRCQN--TAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIP--RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLA-PGVGTDLGFIGCITDIRFGRKTLDLS---KVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRC----EGGTEFYKCFCPWGFGGRNCEKVLPMNASTS--VGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQI-DGRDL---AKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQGREIDFQRDPVSTGNLVP 740
+GG CQ+ + K F C C G+ GS CE PAF+G+S+LA P R H LR +A FRA++T + ++LY+ + DF ++ + DG ++FRF+TGSG A+L S P+ +W+ + L+R + GT+ + G+ PV GESP T GLN+ T +Y GG+ +E+ + +G GCI + + L+LS + V S+ + +C + C PC G CQ CQCP G C + C + PC C GG KC CP G G C+ VL S F G S++EL D + +E+ F P GL+ Y QK +F++L++ + F ++LG+G+A + + + I G +R+ L R RKG++Q+ DG + + S K L+ ++IGG S+L G V GF G + + ++G ++ Q + ++ P
Sbjct: 1231 HGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLR---LALEFRALET-EGLLLYNGNARGK--DFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECG--DHPCLPNPCHGGALCQ-ALEAGMFLCQCPPGRFGPTCADEKSPCQPN-PCHGAAPCRVLSSGGA---KCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNG--QKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSP 1707
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|158705944|sp|Q63HQ2.2|EGFLA_HUMAN (RecName: Full=Pikachurin; AltName: Full=Agrin-like protein; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor) HSP 1 Score: 264.233 bits (674), Expect = 3.457e-74 Identity = 196/711 (27.57%), Postives = 310/711 (43.60%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKS--QGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKD-FECFCPFGQEGSFCEDTTTIERPAFQGN--SYLAIP---RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGG------VNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ-GREIDFQRDPVSTGNLVPC 741
SY+ L +SY + L F+ E +GL+LY +E G DF+S + + R+N G+ AI+ +E I G WHTV + R N L +++ P+ G + G++ + TPLYLGG PS + T N GF+G C+ V + + + P L G D + S GIC ++ CI +GG C D + C CP G +G CED T+ P F+ + SY A P P+H L ++ FR D+ D V+LYS + G DF S+ + GH+EFRF+ GSGT +LRS +P+ W+E+ ++R K G + + Q+ V+G + G + T ++ GG V K L P F G I I +T+ + N+ + + C APC +GG+C+P K+ + C CP F G +C++ +C G C + + A F G S++ D + V + F TT GL+ ++ G + +F++L + G F +NLG G A + + NDG+ R+K +R + G I +D G S G +L+ +G +++GG + + T+ +Y+ G GC+ + I D V N+ C
Sbjct: 395 SYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQG--------CVQSLAVNGRRIDMRPWPLGKALSGADVGECSS-------GICDEAS------CI----------------------HGGTCTAIKADSYICLCPLGFKGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFMEFEITFRP-DSGDGVLLYSYDT--GSKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGVLKP-------FSGSIQKIILNDRTIHVKHDFTSGVNVENAA---HPCVRAPCAHGGSCRP--RKEGYDCDCPLGFEGLHCQK---EC----------------------------GNYCLNTI-IEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWR-GDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTC 1014 HSP 2 Score: 170.244 bits (430), Expect = 1.934e-42 Identity = 124/463 (26.78%), Postives = 209/463 (45.14%), Query Frame = 0
Query: 281 CFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKV----IVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYG-FRGCLHRLRIQGREIDFQRDPVSTGNL 738
C C G+ G C + I+ P F G+SY+ K+ + ++ FRA + D ++LY ++E G+GDF S+ I L+FRFN G+G AI+ S I W+ V L R G + ++ PV G+S G + RTP+Y GG Y L GT+ GF GC+ + + +D+ + A++ +CSS CDEA C +GGTC K+ D + C CP F+G +CE T +++P +F + S+ W L P + +E E+ F G++ Y ++F++++++ G F F+ G G+ L + + G L++ R A+ G +Q+D + + +G ++G ++ + +IFIGG + V F G + ++ + R I + D S N+
Sbjct: 369 CQCTLGKGGESCSEDIVIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRA-EAEDGLLLYCGENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA-PSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMRPWPLGKALSGADVGECSS--GICDEASCIHGGTCTAIKA-DSYICLCPLGFKGRHCEDAFT---LTIP-----------QFRESL--------------------------RSYAATPWPLEPQHYLSFME-FEITFRPDSGDGVLLY----SYDTGSKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGVLKPFSGSIQKIILNDRTIHVKHDFTSGVNV 781 HSP 3 Score: 166.777 bits (421), Expect = 2.532e-41 Identity = 131/460 (28.48%), Postives = 210/460 (45.65%), Query Frame = 0
Query: 34 THVIPHFNQAPLSYME--LVTLPDSYLN-LDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLK-----SQGVDTCDS---CSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILR----QLKMAFRFRAVDTRDSVVLYSAQSE-DGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRK-TLDLSKVIVDSANIRDCSS 476
T IP F ++ SY P YL+ ++ E++F+P+ +G++LY+ + G KDF+S L G E R++ GS T +L +E P+T G WH ++++R+ KN L VD + G A G F + T +++GG P+ +D N+G + F Q+++L+ T+ + GV+ ++ C +PC +GG C+ +GY C C GF G HC++E C G C NT + IE P F G SYL P + R + + RF+ +D ++L+ S DF S+ ++DG L F +N GSG A + +W+ V R ++G + + G+SPG R LNI +Y GG+ ++ L G +GCI+ + L + VD NI C +
Sbjct: 606 TLTIPQFRESLRSYAATPWPLEPQHYLSFMEFEITFRPDSGDGVLLYSY--DTGSKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPN----YDDVKKNSGV-----------LKPFSGSIQKIILNDRTIHVKHDFTSGVNVENAAHPCVRAPCAHGGSCR--PRKEGYDCDCPLGFEGLHCQKE---C------GNYCLNTIIE------------------AIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTT-AKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGM--KEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGA 1016 HSP 4 Score: 59.3066 bits (142), Expect = 9.989e-8 Identity = 48/194 (24.74%), Postives = 84/194 (43.30%), Query Frame = 0
Query: 541 KCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQGREIDFQRDPV 733
+C C G GG +C + + + F G S++ + P + + + LEF GL+ Y + + +FM+L+I + F FN G G A + + I G + L R G +Q++ G S+G +++ +++GG L GF+GC+ L + GR ID + P+
Sbjct: 368 RCQCTLGKGGESCSEDIVIQYPQ---FFGHSYVTFE----PLKNSYQAFQITLEFRAEAEDGLLLYCG--ENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMRPWPL 552
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|158705693|sp|A3KN33.1|EGFLA_BOVIN (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor) HSP 1 Score: 263.848 bits (673), Expect = 4.127e-74 Identity = 197/718 (27.44%), Postives = 311/718 (43.31%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKS--QGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKD-FECFCPFGQEGSFCEDTTTIERPAFQGN--SYLAIP---RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGG------VNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVE-------LEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ-GREIDFQRDPVSTGNLVPC 741
SY+ L +SY + L F+ E +GL+LY +E G DF+S + + R+N G+ AI+ +E I G WHTV + R N L +++ P+ G + G++ + TPLYLGG PS + T N GF+G C+ V + + L P L G D + S GIC ++ CI NGG C + D + C CP G G CED T+ P F+ + SY A P P+H L ++ FR D+ D V+LYS + G DF S+ + GH+EFRF+ GSGT +LRS EP+ W+E+ ++R K G + + Q+ V+G + G + + ++ GG V K L P F G I I +T+ + N+ + + C +PC +GG+C+P K+ + C CP F G +C++ T+ I +P F G S++ D +P ++ V + F TT GL+ ++ G + +F++L + G F +NLG G A + + NDG+ R+K +R + G I +D G S G +L+ +G +++GG + + T+ +Y+ G GC+ + I D V N+ C
Sbjct: 404 SYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGENEHGRGDFMSLAVIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQG--------CVQALTVNGKRLDLRPWPLGKALSGADVGECSS-------GICDEAS------CI----------------------NGGTCMASKADSYICLCPLGFRGRHCEDAFTLTIPQFKESLRSYAATPWPLEPRHYLSFMEFEVTFRP-DSEDGVLLYSYDT--GSKDFLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNYDDVKKNSGILKP-------FSGSIQKIILNDRTIHVKHDFTWGVNVENAA---HPCVGSPCAHGGSCRP--RKEGYECDCPLGFEGLHCQKAITEA-IEIP---------------------------------------QFIGRSYLTYD-------NPDILKRVSGSRSNAFMRFKTTAKDGLLLWR-GDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTC 1015 HSP 2 Score: 172.17 bits (435), Expect = 4.157e-43 Identity = 125/456 (27.41%), Postives = 205/456 (44.96%), Query Frame = 0
Query: 281 CFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKV----IVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYG-FRGCLHRLRIQGREIDFQRD 731
C C G+ G C + I+ P F G+SY+ K+ + ++ FRA + D ++LY ++E G+GDF S+ + L+FRFN G+G AI+ S I W+ V L R G + ++ PV G+S G + RTP+Y GG Y L GT+ GF GC+ + K LDL + A++ +CSS CDEA C NGGTC K+ D + C CP FRG +CE T +++P +F + S+ W L P + +E E+ F G++ Y ++F++++++ G F F+ G G+ L + + + G L + R A+ G +Q+D + +G ++G ++ + +IFIGG + + F G + ++ + R I + D
Sbjct: 378 CHCNLGKGGESCSEDIVIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRA-EAEDGLLLYCGENEHGRGDFMSLAVIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA-PSAYWLVRATGTNRGFQGCVQALTVNGKRLDLRPWPLGKALSGADVGECSS--GICDEASCINGGTCMASKA-DSYICLCPLGFRGRHCEDAFT---LTIP-----------QFKESL--------------------------RSYAATPWPLEPRHYLSFME-FEVTFRPDSEDGVLLY----SYDTGSKDFLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNYDDVKKNSGILKPFSGSIQKIILNDRTIHVKHD 783 HSP 3 Score: 155.992 bits (393), Expect = 6.291e-38 Identity = 125/459 (27.23%), Postives = 201/459 (43.79%), Query Frame = 0
Query: 34 THVIPHFNQAPLSYME--LVTLPDSYLN-LDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLK-----SQGVDTCDS---CSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRP---KHILRQLKMAFRFRAVDTRDSVVLYSAQSE-DGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRK-TLDLSKVIVDSANIRDCSS 476
T IP F ++ SY P YL+ ++ E++F+P+ +G++LY+ + G KDF+S + G E R++ GS T +L +E+P+T G WH + ++R+ KN L VD + G A G F + + +++GG P+ +D N+G + F Q+++L+ T+ + GV+ ++ C SPC +GG C+ +GY C C GF G HC++ T IE P F G SYL P K + AF +D ++L+ S DF S+ ++DG L F +N GSG A + +W+ V R ++G + + G+SPG R LNI +Y GG+ ++ L G +GCI+ + L + VD NI C +
Sbjct: 615 TLTIPQFKESLRSYAATPWPLEPRHYLSFMEFEVTFRPDSEDGVLLYSY--DTGSKDFLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPN----YDDVKKNSGI-----------LKPFSGSIQKIILNDRTIHVKHDFTWGVNVENAAHPCVGSPCAHGGSCR--PRKEGYECDCPLGFEGLHCQK-----------------------------------AITEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGM--KEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGA 1017 HSP 4 Score: 56.9954 bits (136), Expect = 5.311e-7 Identity = 45/194 (23.20%), Postives = 84/194 (43.30%), Query Frame = 0
Query: 541 KCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQGREIDFQRDPV 733
+C C G GG +C + + + F G S++ + P + + + LEF GL+ Y + + +FM+L++ + F FN G G A + + I G + L R G +Q++ G S+G +++ +++GG L GF+GC+ L + G+ +D + P+
Sbjct: 377 RCHCNLGKGGESCSEDIVIQYPQ---FFGHSYVTFE----PLKNSYQAFQITLEFRAEAEDGLLLYCG--ENEHGRGDFMSLAVIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQALTVNGKRLDLRPWPL 561
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|81908066|sp|Q4VBE4.1|EGFLA_MOUSE (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; AltName: Full=Nectican; Flags: Precursor) HSP 1 Score: 258.84 bits (660), Expect = 2.838e-72 Identity = 196/718 (27.30%), Postives = 310/718 (43.18%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKS--QGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKD-FECFCPFGQEGSFCEDTTTIERPAFQGN--SYLAIP---RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGG------VNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVE-------LEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ-GREIDFQRDPVSTGNLVPC 741
SY+ L +SY + L F+ E +GL+LY E G DF+S L R+N G+ AI+ +E I G WHTV + R N L +++ P+ G + G++ + TPLYLGG PS + T N GF+G C+ V +++ + P L G D + S GIC ++ CI +GG C D + C CP G G CED + P F+ + SY A P P+H L + FR D+ D V+LYS + G DF S+ + GH+EFRF+ GSGT +LRS P+ +W+++ ++R K G + + Q+ V+G + G + T ++ GG V K L P F G I I +T+ + N+ + + C APC +GG+C+P K+ + C CP F G NC++ +C G +C + + A F G S++ D +P ++ V + F TT GL+ ++ G + +F++L + G F +NLG G A + + +DG+ R+K +R + G I +D G S G +L+ +G +++GG + + T+ +Y+ G GC+ + I D V N+ C
Sbjct: 395 SYVTFEPLKNSYQAFQVTLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQG--------CVQSLSVNGKKIDMRPWPLGKALNGADVGECSS-------GICDEAS------CI----------------------HGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRP-DSGDGVLLYSYDT--GSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHP-------FSGSIQKIILNDRTIHVKHDFTSGVNVENAA---HPCVGAPCAHGGSCRP--RKEGYECDCPLGFEGLNCQK---EC----------------------------GNHCLNTI-IEAIEIPQFIGRSYLTYD-------NPNILKRVSGSRSNAFMRFKTTAKDGLLLWR-GDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQYLRGLVGCISHFTLSTDYHISLVEDAVDGKNINTC 1014 HSP 2 Score: 172.94 bits (437), Expect = 2.490e-43 Identity = 124/463 (26.78%), Postives = 210/463 (45.36%), Query Frame = 0
Query: 281 CFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKV----IVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGR-YVYGFRGCLHRLRIQGREIDFQRDPVSTGNL 738
C C G+ G C + I+ P F G+SY+ K+ + ++ FRA + D ++LY +SE G+GDF S+ + L FRFN G+G AI+ S I W+ V L R G + ++ PV G+S G + RTP+Y GG Y L GT+ GF GC+ + K +D+ ++ A++ +CSS CDEA C +GGTC K+ D + C CP FRG +CE +++P +F + S+ W L P + E E+ F G++ Y ++F+++++++G F F+ G G+ L + + G+ L++ R A+ G +Q+D + + +G ++G ++ + +IFIGG + ++ F G + ++ + R I + D S N+
Sbjct: 369 CHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRA-EAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA-PSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGADVGECSS--GICDEASCIHGGTCAAIKA-DSYICLCPLGFRGRHCEDAFA---LTIP-----------QFRESL--------------------------RSYAATPWPLEPQHYLSFTE-FEITFRPDSGDGVLLY----SYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVKHDFTSGVNV 781 HSP 3 Score: 166.777 bits (421), Expect = 2.671e-41 Identity = 134/484 (27.69%), Postives = 219/484 (45.25%), Query Frame = 0
Query: 10 DAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYME--LVTLPDSYLNL-DLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLK-----SQGVDTCDS---CSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQL----KMAFRFRAVDTRDSVVLYSAQSE-DGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRK-TLDLSKVIVDSANIRDCSS 476
D+ I +C E + AL IP F ++ SY P YL+ + E++F+P+ +G++LY+ + G KDF+S + G E R++ GS T +L +E P+T GQWH ++++R+ KN L VD + G A G F + T +++GG P+ +++ + + F G Q+++L+ T+ + GV+ ++ C +PC +GG C+ +GY C C GF G +C++E C G C NT + IE P F G SYL P +IL+++ AF +D ++L+ S DF S+ ++DG L F +N GSG A + +W+ V R ++G + + G+SPG R LNI +Y GG+ ++ L G +GCI+ + L + VD NI C +
Sbjct: 586 DSYICLCPLGFRGRHCEDAFAL----TIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSY--DTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGS---------------IQKIILNDRTIHVKHDFTSGVNVENAAHPCVGAPCAHGGSCR--PRKEGYECDCPLGFEGLNCQKE---C------GNHCLNTIIE------------------AIEIPQFIGRSYLTYDNP-NILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGM--KEIALHTNRQYLRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGA 1016 HSP 4 Score: 58.151 bits (139), Expect = 2.151e-7 Identity = 48/194 (24.74%), Postives = 83/194 (42.78%), Query Frame = 0
Query: 541 KCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQGREIDFQRDPV 733
+C C G GG C + + + F G S++ + P + + V LEF GL+ Y + D FM+L++ + F FN G G A + + I G + L R G +Q++ G S+G +++ +++GG L GF+GC+ L + G++ID + P+
Sbjct: 368 RCHCNLGKGGEACSEDIFIQYPQ---FFGHSYVTFE----PLKNSYQAFQVTLEFRAEAEDGLLLYCGESEHGRGD--FMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPL 552
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|460018313|sp|P31696.3|AGRIN_CHICK (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor) HSP 1 Score: 261.151 bits (666), Expect = 4.208e-72 Identity = 212/734 (28.88%), Postives = 324/734 (44.14%), Query Frame = 0
Query: 33 VTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQAL--EDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPA---TLKSQGVDTC--DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACN-GGRCQNTAKD-FECFCPFGQEGSFCEDTTTIER-----PAFQGNSYLAIPRPKHIL---RQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRG----LNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTH---GRYVYGFRGCLHRLRIQGREIDFQRDPVSTGNLVPCS 742
+ + IP F SY+ + +Y + + + F+ +GL+LYN + GKDFIS L G ELR+N GS T ++T++ + G+WH + + R+R++ L VD + + G +P +GL+L T L++GG P A+ E T+ G KG +L V Q L L GV C D C +PC +G C + + C CL ++G C E C C+ C + C CP G+EG FCE T + P F G SYL + + + RQ+ M F A + ++ Y+ Q DG+GDF S+ + DG+LE+R++ G G A+LRS EP+ W V L R + G M I+ E V GESP S + LN++ P Y GG + LA F G + I L + I + I + PCQNGGTC P + + C C F G +CE+ + + G E F GR + + + + LD+ P + EL T QGLI + K L+ +++AL+I G ++LG L ++ IN +K R R+GS+Q+ GSS +L TDG +++GG + S + H Y GF GC+ + + +E+ D ++ ++ CS
Sbjct: 1386 IRYFIPSFGGK--SYLAFKMM-KAYHTVRIAMEFRATELSGLLLYNGQNR--GKDFISLALVGGFVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDGEH-VSGESPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLD--------VNNQMYDLREKGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEM-FHCECLHSYTGPTCADERNPCDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFLTCRQMSMEVVFLA-KSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGA-PDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQKPNPCQNGGTCSP--RLESYECACQRGFSGAHCEKVIIE-----------KAAGDAEAI------AFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSG---KGLERSDYIALAIVDGFVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGMERLS-VAHKLPKAYSTGFIGCIRDVIVDRQELHLVEDALNNPTILHCS 2079 HSP 2 Score: 179.489 bits (454), Expect = 3.553e-45 Identity = 137/484 (28.31%), Postives = 233/484 (48.14%), Query Frame = 0
Query: 256 KREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKY-TLAPGVGTDLGFIGCITDIRFGRKTLDLSKV---IVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRC----EGGTEFYKCFCPWGFGGRNCEKVLPMNASTSV--GFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSS----KGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQG 723
K+ C + C +GG C++ ++F C CP G+ G+ CE P+F G SYLA K +++A FRA + ++LY+ Q+ DF S+ + G +E RFNTGSGT ++ S + KW+++ +NR ++G + + G E V GESP T GLN+ T ++ GG +++ +A +G G I + + DL + ++ + + +C + C PC +G +C +++ H C+C + G C CD + PC C EGG C CP G G CE+V + + F G S++EL+ L F + ++E+ F P G+I+Y QK +F++L++ G + ++LG+G+A L + + + + + L R RKG ++I+ + G S K L+ ++GG S+L + F G + R+ I+G
Sbjct: 1345 KKPSRPCDSHPCLHGGTCEDDGREFTCRCPAGKGGAVCEKPIRYFIPSFGGKSYLAFKMMK-AYHTVRIAMEFRATEL-SGLLLYNGQNRG--KDFISLALVGGFVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDG-EHVSGESPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLDVNNQMYDLREKGSDVLYGSGVGECGN--DPCHPNPCHHGASCHVKEAEMFH-CECLHSYTGPTCADERNPCDPT-PCHISATCLVLPEGGA---MCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELN-GLQTLFLTCRQMSMEVVFLAKSPSGMIFYNG--QKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGAPDFSKLARAAAISTSFYGAVQRISIKG 1813 HSP 3 Score: 151.369 bits (381), Expect = 3.510e-36 Identity = 111/448 (24.78%), Postives = 200/448 (44.64%), Query Frame = 0
Query: 37 IPHFNQAPLSYMELVTLPDSYLN---LDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGR----FEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHIL-RQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS 476
+P FN SY+EL L +L + +E+ F + +G+I YN G DF+S L +G E RY+LG A+L +++P+ W +V + RS + + +++ + G +P L+L P Y+GG P + +++ F G + I + ++ + S + + C +PC+NGG C S + Y C C GFSG HC++ + A G + A D + + + + + E PA Y A P K + +++ + A ++L+S + + + D+ ++ I DG ++ ++ GS +LRS PI W + R + G++ + + P+ G SP L+ ++ GG+ + GFIGCI D+ R+ L L + +++ I CS+
Sbjct: 1652 LPEFNG--FSYLELNGLQTLFLTCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGAPDFSKLARAAAISTSFYGAVQRIS---IKGVPLLKEQHIRSAVEISTFRAHPCTQ-KPNPCQNGGTC--SPRLESYECACQRGFSGAHCEK---VIIEKA--AGDAEAIAFDGRTYMEY--HNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQ---GLILWSGKGLE-RSDYIALAIVDGFVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGMERLSVAHKLPKAYSTGFIGCIRDVIVDRQELHLVEDALNNPTILHCSA 2080
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|221271989|sp|B4F785.1|EGFLA_RAT (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor) HSP 1 Score: 257.299 bits (656), Expect = 8.464e-72 Identity = 197/712 (27.67%), Postives = 307/712 (43.12%), Query Frame = 0
Query: 46 SYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKS--QGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKD-FECFCPFGQEGSFCEDTTTIERPAFQGN--SYLAIP---RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGG------VNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSV-GFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ-GREIDFQRDPVSTGNLVPC 741
SY+ L +SY + L F+ E +GL+LY E G DF+S L R+N G+ AI+ +E I G WH+V + R N L +++ P+ G + G++ + TPLYLGG PS + T N GF+G C+ V +++ + P L G D + S GIC ++ CI NGG C D + C CP G G CED T+ P F+ + SY A P P+H L + FR D+ D V+LYS + DF S+ + GH+EFRF+ GSGT +LRS + + +W+++ ++R K G + + Q+ V+G + G + T ++ GG V K L P F G I I +T+ + N+ + + C APC +GG+C+P K+ + C CP F G NC++ T+ I +P F GR+ N V G R ++FM F TT GL+ ++ G + +F++L + G F +NLG G A + + +DG+ R+K +R + G I +D G S G +L+ +G +++GG + + T+ +Y+ G GC+ + I D V N+ C
Sbjct: 391 SYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQG--------CVQSLAVNGKKIDMRPWPLGKALNGADVGECSS-------GICDEAS------CI----------------------NGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRP-DSGDGVLLYSYDTS--SKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHP-------FSGSIQKIILNDRTIHVRHDFTSGVNVENAA---HPCVGAPCAHGGSCRP--RKEGYECDCPLGFEGLNCQKAITEA-IEIP--------------------QFIGRSYLTYDNPNILKRVSGSRSNAFM--------------------RFKTTAKDGLLLWR-GDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALRTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTC 1002 HSP 2 Score: 176.407 bits (446), Expect = 1.675e-44 Identity = 126/463 (27.21%), Postives = 210/463 (45.36%), Query Frame = 0
Query: 281 CFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKV----IVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGR-YVYGFRGCLHRLRIQGREIDFQRDPVSTGNL 738
C C G+ G C + I+ P F G+SY+ K+ + ++ FRA + D ++LY +SE G+GDF S+ + L FRFN G+G AI+ S I W+ V L R G + ++ PV G+S G + RTP+Y GG Y L GT+ GF GC+ + K +D+ ++ A++ +CSS CDEA C NGGTC K+ D + C CP FRG +CE T +++P +F + S+ W L P + E E+ F G++ Y ++F+++ +++G F F+ G G+ L + + G+ L++ R A+ G +Q+D + + +G ++G ++ + +IFIGG + ++ F G + ++ + R I + D S N+
Sbjct: 365 CHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRA-EAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA-PSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSS--GICDEASCINGGTCAAIKA-DSYICLCPLGFRGRHCEDAFT---LTIP-----------QFRESL--------------------------RSYAATPWPLEPQHYLSFTE-FEITFRPDSGDGVLLY----SYDTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNV 777 HSP 3 Score: 152.14 bits (383), Expect = 1.303e-36 Identity = 122/460 (26.52%), Postives = 203/460 (44.13%), Query Frame = 0
Query: 34 THVIPHFNQAPLSYME--LVTLPDSYLNL-DLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLK-----SQGVDTCDS---CSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQL----KMAFRFRAVDTRDSVVLYSAQSE-DGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRK-TLDLSKVIVDSANIRDCSS 476
T IP F ++ SY P YL+ + E++F+P+ +G++LY+ + KDF+S + G E R++ GS T +L +E +T GQWH ++++R+ KN L VD + G A G F + T +++GG P+ +++ + + F G Q+++L+ T+ + GV+ ++ C +PC +GG C+ +GY C C GF G +C++ T IE P F G SYL P +IL+++ AF +D ++L+ S DF S+ ++DG L F +N GSG A + +W+ V R ++G + + G+SPG R LNI +Y GG+ ++ L G +GCI+ + L + VD NI C +
Sbjct: 602 TLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSY--DTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGS---------------IQKIILNDRTIHVRHDFTSGVNVENAAHPCVGAPCAHGGSCR--PRKEGYECDCPLGFEGLNCQK-----------------------------------AITEAIEIPQFIGRSYLTYDNP-NILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGM--KEIALRTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGA 1004 HSP 4 Score: 57.3806 bits (137), Expect = 3.795e-7 Identity = 47/194 (24.23%), Postives = 83/194 (42.78%), Query Frame = 0
Query: 541 KCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQGREIDFQRDPV 733
+C C G GG C + + + F G S++ + P + + + LEF GL+ Y + D FM+L++ + F FN G G A + + I G + L R G +Q++ G S+G +++ +++GG L GF+GC+ L + G++ID + P+
Sbjct: 364 RCHCNLGKGGEACSEDIFIQYPQ---FFGHSYVTFE----PLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGD--FMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPL 548
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Match: gi|460018339|sp|O00468.5|AGRIN_HUMAN (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor) HSP 1 Score: 259.61 bits (662), Expect = 1.469e-71 Identity = 218/754 (28.91%), Postives = 339/754 (44.96%), Query Frame = 0
Query: 16 CKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTP--SQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQ--EMVLSP-ATLKSQGVDTC--DSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGR-CQNTAK-DFECFCPFGQEGSFCEDTTTIERPA-----FQGNSYLAIPRPKHILRQL--KMAFRFRAVDTRDS-VVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTR----GLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEA---PCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGG----GIKPSRLTHGRYVYGFRGCLHRLRIQGREIDFQRDPVSTGNLVPC 741
C A A E+ ++ +P F S++ TL +Y L L L F+ GL+LYN + GKDF++ L +G+ +LR++ GS A+LT+ P+ GQWH ++++R + +L VD + P+ G +P +GL+L T L++GG P ALE T + G +G CI V Q E+ + P A + GV C C +PC G CQ + + C C PG G C E C C+G C+ + +C CP G+EG+FC+ + + F G S+L + R L KMA + S ++LY+ Q DG+GDF S+ ++D LEFR++ G G A++RS EP+ W V+L R + G + + V GESP S + LN++ P+Y GG + LA GF G I + G + L + ++ ++ S C A PC NG +C P ++ + C CP F G +CE+ + + G + F GR + L + + LD L H + EL T QGL+ + K + +++AL+I G +NLG L ++ +N + LR+ R+ R+GS+Q+ GSS +L TDG +++GG + P+ Y GF GCL + + + D V+ L PC
Sbjct: 1357 CPAGRGGAVCEK----VLGAPVPAFEG--RSFLAFPTL-RAYHTLRLALEFRALEPQGLLLYNGNAR--GKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRG--------CIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGR-FHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGA-PDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVT--SFAGHPCTRASGHPCLNGASCVPREAA--YVCLCPGGFSGPHCEKGLVE-----------KSAGDVDTL------AFDGRTFVEYLNAVTESELANEIPVPETLDSGAL-HEKALQSNHFELSLRTEATQGLVLWSG---KATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPA--LPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPC 2064 HSP 2 Score: 181.8 bits (460), Expect = 6.640e-46 Identity = 142/478 (29.71%), Postives = 230/478 (48.12%), Query Frame = 0
Query: 268 NGGRCQNTA--KDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIP--RPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLA-PGVGTDLGFIGCITDIRFGRKTLDL---SKVIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRC----EGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHF--DPTKIETVELEFSTTEPQGLIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQI-DGRDL---AKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYV-YGFRGCLHRLRIQGREI 726
+GG CQ+ A F C CP G+ G+ CE PAF+G S+LA P R H LR +A FRA++ + ++LY+ + DF ++ + DG ++ RF+TGSG A+L S P+ +W+ + L+R + GT+ + G+ PV GESP T GLN+ T ++ GGV +++ +A G GCI + + L+L + + +C + C PC G CQ ++ H CQCP G C + C + PC C EGG + C CP G G C+ + S + F L+ + L F D + +E+ F P GL+ Y QK +F++L++ F ++LG+G+A + + + + G R+ L R RKG++++ DG + + S K L+ +++GG S+L V GF G + + + GR++
Sbjct: 1340 HGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLR---LALEFRALEPQ-GLLLYNGNARGK--DFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECG--DHPCLPNPCHGGAPCQNLEAGRFH-CQCPPGRVGPTCADEKSPCQPN-PCHGAAPCRVLPEGGAQ---CECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNG--QKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQL 1802
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1330874662|gb|PNF14541.1| (hypothetical protein B7P43_G15032 [Cryptotermes secundus]) HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0 Identity = 340/776 (43.81%), Postives = 481/776 (61.98%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQ------DGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELT--ASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSKDDQLDNGDQTDLEE 757
LTIP V + DAG Y+C A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE ++GLILYN + GG DF+S GL N E R+++GS A++ ++ PI G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P + N GF G CI R ++G+ L +QGV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG++G+ CE+ I PAF ++Y++ P PK LR+LKMA +F D +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS + +W V++ R + G + ++ PV G+SPG TRGLN++TP+Y GG +K++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DC S T S CD PC NGGTC+ S + C C + F G +CE A C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH PT+ E + LEFSTTEP GLI++ DG +Q +++AL++ G F F LG G A++ A ++DGKR + L R+A GSI++D G S+G L+T GNI+IGG R+T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+ D + + LE+
Sbjct: 2 LTIPDVRAVDAGTYVCTARNRNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESSDGLILYNGQQKEGGGDFVSLGLNNSYVEFRFDVGSGPAVIRSDHPIRIGEWHTVKLSRTRKQGTMYVDGNGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLSGFNRGFVG--------CISRLLIGSVHHELVRDATLNQGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKQGAHCENDIDISEPAFADDAYVSYPTPK-ALRRLKMALKFNPADAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSEREVTPGEWTTVSVGRNFREGQIIVNDGAPVVGQSPGQTRGLNLKTPLYVGGYDKQRIRLADGVGVKKGFSGCVREIQMSGEKLDIVGSVVDAANVQDCGSLYRRNHYTTASPCDRNPCLNGGTCRLIDSIGHYNCTCQDGFSGQHCELEADLCVALYPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTPTENEVITLEFSTTEPNGLIFWHGQTPDTDGRTQQ----DYVALAVVDGILEFSFELGSGPAQIQTRADTRVDDGKRHHVILRRQASDGSIELDSI-TEFGESQGILKMLNTRGNIYIGGLPDFDRMTGGKYLRGFVGCIHSLLIQESSGPGLNFREEALYSVNALPCTNDPDVADNSLGGLED 761
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1022773707|gb|KZS17754.1| (Uncharacterized protein APZ42_016204 [Daphnia magna]) HSP 1 Score: 662.529 bits (1708), Expect = 0.000e+0 Identity = 331/749 (44.19%), Postives = 468/749 (62.48%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDL-PIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRP-KHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSSTESKCDEAPCQNGGTC-QPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQG-LIWYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSS-KGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ-GREIDFQRDPVSTGNLVPCSK 743
L+IP V ++DAG Y+C A N++ ++ L+VT V+P+F QAPLSYM++ TLP++Y+ D+E+SFKPE +GLILYN G DFISFGL NG PE R+++G+ AI+ ++K I G+WHTVK++R+RKN S+ VD+D GSA GRF GLDL+ PLY+GG P+ + + GF G CIGR VVG+ L S+G+ TC++C+L+PCENGG+CQ + QGY CIC GFSG +C + GE C+ GAC GRC N A FEC CP G+ G CE I PAF S++A P P K+ LR+++M+ R + +DS++ Y AQ+E+GQGDF+S+ I+D +EFRF++GSG AILRS I A +W ++ +R + G +F++ V+G+SPGSTRGLN+ T ++ GG +++ L+PGV F GC++ LDL ++DSAN+ DC S C+ PC NGG C + G S + CQC E F G +CE A C + PCQNGG C G YKC CP GFGG NCE+ ++ + F+GDS++E+D L PH ET+ +EFST EP G L+W+ + + ++F++L++ G F + LG G ++ ++DGKR R R AR GS+++DG L G S +G L+T GNI++GG +T GRY++G +GC+H L IQ I+FQR +S N+VPCS+
Sbjct: 160 LSIPEVRAEDAGTYVCTARNAEETMDLPTVLVVTGVVPYFAQAPLSYMQMPTLPNAYMEFDIEISFKPESPDGLILYNGQQAGGSGDFISFGLNNGYPEFRFDVGAGPAIMKSDKAIDMGKWHTVKLSRNRKNGSMTVDEDGGTFHGSATGRFLGLDLIEPLYIGGVPNIRSIHKLAGFSRGFVG--------CIGRLVVGSSAHELVRDATNSRGITTCETCALNPCENGGVCQEAYTQQGYTCICPSGFSGLNCDKIGETCYPGACGMGRCVNMAGGFECQCPLGKTGMNCEKEIIIYEPAFTEGSFIAYPTPPKNALRKMRMSMRIKPRQIKDSLLAYCAQTEEGQGDFSSLAIKDKRVEFRFDSGSGPAILRSDREIRAGEWVQITADRSFRDGVLFVNDGSEVRGKSPGSTRGLNLNTRLFVGGWDRQHVRLSPGVNVTSSFDGCLSQFEVQGMELDLVNSVIDSANVEDCGGA-SACERKPCANGGLCTETGSSLTDYMCQCEEGFSGKSCEIEADLCQVIRPCQNGGSCIGTYNAYKCNCPMGFGGSNCER--NVDVAEEAFFQGDSYVEMDKALFPHASSNTAETITVEFSTLEPNGILMWHGQKPETISRGQDFVSLTVVDGRLEFSYELGSGPVQIITPVRVDDGKRHRAVARRTARDGSLELDGVILENGRSPEGPHQILNTKGNIYVGGLPNAEAMTTGRYIHGLKGCIHHLEIQESGVINFQRAALSAINVVPCSR 897
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1339077218|ref|XP_023725986.1| (basement membrane-specific heparan sulfate proteoglycan core protein-like [Cryptotermes secundus]) HSP 1 Score: 662.144 bits (1707), Expect = 0.000e+0 Identity = 338/762 (44.36%), Postives = 476/762 (62.47%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQ------DGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAELT--ASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
LTIP V + DAG Y+C A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE ++GLILYN + GG DF+S GL N E R+++GS A++ ++ PI G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P + N GF G CI R ++G+ L +QGV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG++G+ CE+ I PAF ++Y++ P PK LR+LKMA +F D +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS + +W V++ R + G + ++ PV G+SPG TRGLN++TP+Y GG +K++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DC S T S CD PC NGGTC+ S + C C + F G +CE A C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH PT+ E + LEFSTTEP GLI++ DG +Q +++AL++ G F F LG G A++ A ++DGKR + L R+A GSI++D G S+G L+T GNI+IGG R+T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3493 LTIPDVRAVDAGTYVCTARNRNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESSDGLILYNGQQKEGGGDFVSLGLNNSYVEFRFDVGSGPAVIRSDHPIRIGEWHTVKLSRTRKQGTMYVDGNGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLSGFNRGFVG--------CISRLLIGSVHHELVRDATLNQGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKQGAHCENDIDISEPAFADDAYVSYPTPK-ALRRLKMALKFNPADAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSEREVTPGEWTTVSVGRNFREGQIIVNDGAPVVGQSPGQTRGLNLKTPLYVGGYDKQRIRLADGVGVKKGFSGCVREIQMSGEKLDIVGSVVDAANVQDCGSLYRRNHYTTASPCDRNPCLNGGTCRLIDSIGHYNCTCQDGFSGQHCELEADLCVALYPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTPTENEVITLEFSTTEPNGLIFWHGQTPDTDGRTQQ----DYVALAVVDGILEFSFELGSGPAQIQTRADTRVDDGKRHHVILRRQASDGSIELDSI-TEFGESQGILKMLNTRGNIYIGGLPDFDRMTGGKYLRGFVGCIHSLLIQESSGPGLNFREEALYSVNALPCTK 4238
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|646722981|gb|KDR23766.1| (Basement membrane-specific heparan sulfate proteoglycan core protein [Zootermopsis nevadensis]) HSP 1 Score: 660.988 bits (1704), Expect = 0.000e+0 Identity = 336/771 (43.58%), Postives = 474/771 (61.48%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSKD-DQLDNGDQTDLE 756
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+ D D DN LE
Sbjct: 2714 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTNDPDFADNNILEGLE 3472
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1227958575|ref|XP_021927474.1| (basement membrane-specific heparan sulfate proteoglycan core protein isoform X22 [Zootermopsis nevadensis]) HSP 1 Score: 659.062 bits (1699), Expect = 0.000e+0 Identity = 331/757 (43.73%), Postives = 469/757 (61.96%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3118 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTK 3862
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1227958557|ref|XP_021927394.1| (basement membrane-specific heparan sulfate proteoglycan core protein isoform X13 [Zootermopsis nevadensis]) HSP 1 Score: 658.677 bits (1698), Expect = 0.000e+0 Identity = 331/757 (43.73%), Postives = 469/757 (61.96%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3247 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTK 3991
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1227958569|ref|XP_021927448.1| (basement membrane-specific heparan sulfate proteoglycan core protein isoform X19 [Zootermopsis nevadensis]) HSP 1 Score: 658.677 bits (1698), Expect = 0.000e+0 Identity = 331/757 (43.73%), Postives = 469/757 (61.96%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3206 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTK 3950
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1227958573|ref|XP_021927465.1| (basement membrane-specific heparan sulfate proteoglycan core protein isoform X21 [Zootermopsis nevadensis]) HSP 1 Score: 658.677 bits (1698), Expect = 0.000e+0 Identity = 331/757 (43.73%), Postives = 469/757 (61.96%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3168 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTK 3912
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1227958541|ref|XP_021927326.1| (basement membrane-specific heparan sulfate proteoglycan core protein isoform X5 [Zootermopsis nevadensis]) HSP 1 Score: 658.677 bits (1698), Expect = 0.000e+0 Identity = 331/757 (43.73%), Postives = 469/757 (61.96%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3284 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTK 4028
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Match: gi|1227958571|ref|XP_021927458.1| (basement membrane-specific heparan sulfate proteoglycan core protein isoform X20 [Zootermopsis nevadensis]) HSP 1 Score: 658.677 bits (1698), Expect = 0.000e+0 Identity = 331/757 (43.73%), Postives = 469/757 (61.96%), Query Frame = 0
Query: 1 LTIPRVTSDDAGIYICKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKPELANGLILYNAHDERGGKDFISFGLKNGKPELRYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGTPSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCDSCSLSPCENGGICQGSANDQGYRCICLPGFSGEHCKREGEMCFTGACNGGRCQNTAKDFECFCPFGQEGSFCEDTTTIERPAFQGNSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSEDGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGVGTDLGFIGCITDIRFGRKTLDLSKVIVDSANIRDCSS--------TESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLI-WYQDGVQKQLKDRNFMALSISSGTPSFDFNLGEGSAEL--TASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPSRLTHGRYVYGFRGCLHRLRIQ---GREIDFQRDPVSTGNLVPCSK 743
L+IP V + DAG YIC A N + +++ L+VT V+P+F QAP S+M L TLPD+YL ++E+SFKPE +GLILYN + GG DF+S GL N E R+++GS AI+ +++PI+ G+WHTVK++R+RK +++VD + P G+A GRF+GLDL+ PLY+GG P GF G CI R ++G+ L S GV TC++C+++PC N G+CQ + QGY CIC PGFSG +C R GE CF GAC GRC N + FEC CPFG+ G+ CE+ I PAF +Y++ P PK + R+LKMA +F VD +D +++Y AQSEDGQGDF S+ I+D LEFRF+TGSG A++RS I +W V++ R + G + ++ P+ G+SPG TRGLN++TP+Y GG ++++ LA GVG GF GC+ +I+ + LD+ +VD+AN++DCSS T S CD PC NGGTC+ S + C C E F G +CE C PC NGG+C G YKC CP G+GG NCE+ S+ V F+GD ++EL LLPH T+ E + +EFSTTEP GLI W+ + ++++AL++ +G F + LG G A++ A ++DGKR + L R+ GSI++D G S+G L+T GNI+IGG +T G+Y+ GF GC+H L IQ G ++F+ + + + N +PC+K
Sbjct: 3195 LSIPDVQAQDAGTYICTARNKNVKMDIPTVLVVTGVVPYFAQAPTSFMALPTLPDAYLQFNIEVSFKPESTDGLILYNGQQKGGGGDFVSLGLNNSHVEFRFDVGSGPAIIKSDRPISLGEWHTVKLSRTRKQGTMYVDGEGPYSGTALGRFQGLDLVEPLYVGGVPDFRNIHRLNGFTRGFVG--------CISRLLIGSVHHELVRDATLSHGVTTCETCAVNPCLNQGVCQEATELQGYSCICPPGFSGLNCDRVGEACFPGACGVGRCINRDEGFECLCPFGKHGAHCENDIDISEPAFADGAYVSYPTPKAV-RRLKMALKFNPVDAQDGLLMYCAQSEDGQGDFTSLAIRDKRLEFRFDTGSGPAVIRSERDITPGEWTSVSVGRSFREGQITVNDDSPIVGQSPGHTRGLNLKTPLYVGGYDEQRIRLADGVGVKKGFSGCVREIQLSGEKLDIISSVVDAANVQDCSSLYSRNHYVTSSPCDRNPCLNGGTCRLTDSSGHYNCTCQEGFSGQHCEMETDLCAALHPCHNGGQCVGTPNSYKCRCPTGYGGTNCEQAAEF--SSEVSFQGDGYIELSNSLLPHKTATENEVITIEFSTTEPNGLIFWHGQTSDTDGRTQDYVALAVVNGLLEFSYELGSGPAQIQTQADARVDDGKRHHVVLKRQGSDGSIELDSI-TEFGESQGVLKMLNTRGNIYIGGLPDFDHMTGGKYLRGFVGCIHSLLIQDTSGSGLNFRENALYSVNALPCTK 3939 The following BLAST results are available for this feature:
BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 21
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BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of basement membrane-specific heparan sulfate proteoglycan core protein vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold48_size466083:89031..99393+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold48_size466083-snap-gene-1.19 ID=maker-scaffold48_size466083-snap-gene-1.19|Name=basement membrane-specific heparan sulfate proteoglycan core protein|organism=Tigriopus kingsejongensis|type=gene|length=10363bp|location=Sequence derived from alignment at scaffold48_size466083:89031..99393+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'basement membrane-specific heparan sulfate proteoglycan core protein' has the following synonyms
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